LOCUS       BGC0000719             12866 bp    DNA     linear   BCT 14-JUL-2016
DEFINITION  Streptoalloteichus hindustanus tobramycin biosynthetic gene cluster
            (alloA, alloB, alloC, alloD,alloE, alloF, alloG, alloH, alloI),
            partial and complete cds.
ACCESSION   BGC0000719
VERSION     BGC0000719
KEYWORDS    .
SOURCE      Streptoalloteichus hindustanus
  ORGANISM  Streptoalloteichus hindustanus
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Streptoalloteichus.
COMMENT     ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2024-10-17 14:05:54
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 12866
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     gene            <1..1687
                     /gene="alloA"
     source          1..12866
                     /db_xref="taxon:2017"
                     /mol_type="genomic DNA"
                     /organism="Streptoalloteichus hindustanus"
                     /strain="JCM 3268"
                     /type_material="type strain of Streptoalloteichus
                     hindustanus"
     subregion       1..12866
                     /aStool="mibig"
                     /contig_edge="True"
                     /label="saccharide"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..12866
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     CDS             <1..1687
                     /codon_start=2
                     /function="tobramycin biosynthesis (maybe outside of gene
                     cluster)"
                     /gene="alloA"
                     /product="putative polyvinylalcohol dehydrogenase"
                     /protein_id="BAE98075.1"
                     /transl_table=11
                     /translation="MRRHRDQGDFRACHRTKAYSTPRVSRRAAGRAAADQRREPAADPA
                     ADAPEAFSGASWAADWPTWQKDLSGSRSNRAERRITPDNVENLRLKWAFTHPQPKGLPD
                     GVRLTARSQPAVVNGVAYFGSTDGKFYAVNARTGKTKWELDLNEVNSGSGFTAVRNSPA
                     VSGGKIFFGDYAGHLYAVDQKSGELVWSTKIDNHPQGLTTSSPIVHGGRVYVGVSSAEN
                     TGGIETACCTFRGHVDALDANTGALVWRHYTVPEAKAVGTWPNGATRYEPSGGGVWSSP
                     VIDPSTRTLYVGTGQNYTGKEGETDTVLALDIANGEVRWKRQMTFPDVWRQVCADPSAP
                     PGYCPGAKEGAALDYDFGAMPNIFSANGRKLVGIGQKNGMYHVFDAHTGEVVWQRQLGK
                     PWPNGGVGGIQWGASYDGKRLYVATWFADPGSLFALDPATGRTLWETKHPANGCSTGGA
                     AKHPEVCALANTPAVSTSPGLVYEGSADGKMRAYSAENGKVLWEFDTIREFQGVNGLTG
                     HGQGISGGAVIADGVLYVNSGYFGQYGRFYPTDKGSVLLAFSLR"
     gene            2604..3623
                     /gene="alloB"
     CDS             2604..3623
                     /NRPS_PKS="Domain: PKS_ER (111-254). E-value: 5.6e-11.
                     Score: 33.9. Matches aSDomain:
                     nrpspksdomains_alloB_PKS_ER.1"
                     /codon_start=1
                     /function="tobramycin biosynthesis"
                     /gene="alloB"
                     /gene_functions="biosynthetic (rule-based-clusters) 2dos:
                     ADH_N"
                     /gene_functions="biosynthetic (rule-based-clusters) 2dos:
                     ADSI-DH"
                     /gene_functions="biosynthetic (rule-based-clusters) 2dos:
                     ADH_zinc_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1040:alcohol dehydrogenase (Score: 313.3; E-value:
                     2.8e-95)"
                     /gene_kind="biosynthetic"
                     /product="putative dehydrogenase"
                     /protein_id="BAE98076.1"
                     /sec_met_domain="ADSI-DH (E-value: 1e-161, bitscore: 526.6,
                     seeds: 6, tool: rule-based-clusters)"
                     /sec_met_domain="ADH_N (E-value: 3.3e-28, bitscore: 87.1,
                     seeds: 66, tool: rule-based-clusters)"
                     /sec_met_domain="ADH_zinc_N (E-value: 5e-23, bitscore:
                     70.9, seeds: 86, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKALTFVAPGEAVFGTRAVPVPAPGEALVRLGYNSICGSDLSLYR
                     GVWHGFGYPVVPGHEWSGTVVEVNGPGDELVGRDVVGDLTCPCGACAACQRGTPVLCEN
                     LQELGFTRDGACAEYMTIPTRNLHRLPAELSLRVACQVEPVAVALHALSTVDVSPGEKV
                     AVLGAGGIGLLLSQVARHRGAVITTVGEPVPERRAVAAQLGAATVTTGRPGELADLVAK
                     RPELTPDVVLEASGYPVALQEAIEVVRPGGRIGVVGYRVEEISPMATHHVAVKALTIRG
                     SLGPGNRFPDAIDLLARREINVDPLLSHEFALADHARALELALRRTEGNIRSFFNLRA"
     aSDomain        2937..3365
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_alloB_PKS_ER.1"
                     /evalue="5.60E-11"
                     /label="alloB_PKS_ER.1"
                     /locus_tag="alloB"
                     /protein_end="254"
                     /protein_start="111"
                     /score="33.9"
                     /tool="antismash"
                     /translation="RDGACAEYMTIPTRNLHRLPAELSLRVACQVEPVAVALHALSTVD
                     VSPGEKVAVLGAGGIGLLLSQVARHRGAVITTVGEPVPERRAVAAQLGAATVTTGRPGE
                     LADLVAKRPELTPDVVLEASGYPVALQEAIEVVRPGGRI"
     gene            3733..5031
                     /gene="alloC"
     CDS             3733..5031
                     /codon_start=1
                     /function="tobramycin biosynthesis"
                     /gene="alloC"
                     /product="putative transporter"
                     /protein_id="BAE98077.1"
                     /transl_table=11
                     /translation="MSQHAASADETSAGAPAADPSPESRARARATGRRLRLFFGLHELV
                     PLFPLYPLLFADHGLSTAGISGLLALWSVTAFVLEVPSGVLGDMFSRRWLLAVGTAVRA
                     CGFGLWVAFPSPWAFAAGFLCWGLCDALTSGTLESLIYDELRAAGASHRYAAVMGSGRA
                     VSLLSTLTAMTLAGPVFALGGYPLVGMISLGSGLACAVAALRFSEPPRDQVGREDGGLR
                     WYVATFRAGVSEVRRDRVVLRAVLIAASLHSMTAIDEYFPMLARANGASTTMVPVLVAV
                     TVVCAAAGSGLVGRTAHAPPAVLGLALLASAGLMVAGALSGLQAGMLAVGVSFGLVQWA
                     LVLADTRLQDAITGPARATVTSVVGFGAEVASVGFYLGAGALSTVTSTSELLAWFAVPL
                     VLIAVAAPRWLRREPQPPGEVPRAPGAEPPRPA"
     gene            5208..6395
                     /gene="alloD"
     CDS             5208..6395
                     /NRPS_PKS="Domain: Aminotran_3 (42-343). E-value: 3.7e-41.
                     Score: 133.1. Matches aSDomain:
                     nrpspksdomains_alloD_Aminotran_3.1"
                     /codon_start=1
                     /function="tobramycin biosynthesis"
                     /gene="alloD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013:aminotransferase class-III (Score: 269.6;
                     E-value: 7.4e-82)"
                     /gene_kind="biosynthetic-additional"
                     /product="putative aminotransferase"
                     /protein_id="BAE98078.1"
                     /sec_met_domain="Aminotran_3 (E-value: 2.6e-43, bitscore:
                     137.4, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTQTNEISATTGDWSRSAEHLRRSHEVTTNPRPDQDGQFPCVLSR
                     GAGARVYDLDDNAYFDLTCSFGTVLIGHAEPAVTDAIRASLDDGNLFYTGPSPRRLALA
                     ERLLDWFPWADQALFYRTGSCAVSAVARLTQHVTGRTTVLTSGYHGWHDWHLEAVPEAK
                     LFPSYATEFHEDLDVYRAYLERHADEIAAVVVTPEPTRKPVGHYRVLRELAAEHGCLFV
                     VDEVKTGMRAGKGGLSALAGLEPDAVTVSKGLANGHSISAVVGSRRITDGLDEAHVWST
                     YQNEQVGFAAALATVDFLESQDVAAVVGRTGRVVEQAFRSAFAERGLPVEVRGWGPMFD
                     LDFSAAEEGLADRLQLAFLRHGVFCDIGDDFNVMYRTADHLDELTGRVTAAIRSV"
     aSDomain        5334..6236
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_alloD_Aminotran_3.1"
                     /evalue="3.70E-41"
                     /label="alloD_Aminotran_3.1"
                     /locus_tag="alloD"
                     /protein_end="343"
                     /protein_start="42"
                     /score="133.1"
                     /tool="antismash"
                     /translation="LSRGAGARVYDLDDNAYFDLTCSFGTVLIGHAEPAVTDAIRASLD
                     DGNLFYTGPSPRRLALAERLLDWFPWADQALFYRTGSCAVSAVARLTQHVTGRTTVLTS
                     GYHGWHDWHLEAVPEAKLFPSYATEFHEDLDVYRAYLERHADEIAAVVVTPEPTRKPVG
                     HYRVLRELAAEHGCLFVVDEVKTGMRAGKGGLSALAGLEPDAVTVSKGLANGHSISAVV
                     GSRRITDGLDEAHVWSTYQNEQVGFAAALATVDFLESQDVAAVVGRTGRVVEQAFRSAF
                     AERGLPVEVRGWGPMFDLDF"
     gene            6448..7974
                     /gene="alloE"
     CDS             6448..7974
                     /codon_start=1
                     /function="tobramycin biosynthesis"
                     /gene="alloE"
                     /product="putative dehydrogenase"
                     /protein_id="BAE98079.1"
                     /transl_table=11
                     /translation="MLLISPDEAARRTYDACVIGSGASGSVAAAVLAERGLSVLVVEQG
                     GPIPPGADGEDHEDPDGWAFARRDGRWTRDGYPWTAMALGGGTLFYAGMSYRYQQTDLA
                     PPSSFLGAADYDHWQVGLADLEPHYEWIEYQLAIAGPSRKQVGDYVFPVYHRPSLPLTP
                     PGAALAEGARSLHLTPLPTPVAISGVRDRFGPGCAQTTPCTSFSCPTKAKADVVSRVLA
                     PAEGDVSVVVHTRVVRLVASSPHRVDAVEVLDRENGTRLTLRARRFLLAANAIQSAALL
                     LRSTTRTEPDGIGNRHGLVGRHLAMKNSVYVRGTIPRRLPGYTPLRHRYSSVCVLDFLT
                     GEAFPDGIGGLIHEANPWEPAAEREAARGTLLQVECLVGDRPQAENRVRLSRTRDRDGL
                     PRIVMDYRPHPADLRRLAVLSERAHDILTKAGAVDVREVDTAYELGSAHLHGTLRAGRD
                     PRTSVTDPFGRLHDHDNVWSVDGATFPFAGNPNPTLTIQANAHRIALAID"
     gene            8025..9737
                     /gene="alloF"
     CDS             8025..9737
                     /codon_start=1
                     /function="tobramycin biosynthesis"
                     /gene="alloF"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Carbam_trans_N"
                     /gene_kind="biosynthetic-additional"
                     /product="putative carbamoyl transferase"
                     /protein_id="BAE98080.1"
                     /sec_met_domain="Carbam_trans_N (E-value: 1.8e-29,
                     bitscore: 92.4, seeds: 4, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRVLGLNGWPRDFHDASAALLVDGRITAFAEEERFTRKKHGYDTA
                     PAQAAAFCLAQAGLTVDDLDAVAFGWDLPRMYRERLGGWPHSDSEALDILLPRHLFPRR
                     TDPPLHFVQHHVAHAASAYHFSGVDHGAVLIVDGEGEEECVTLARAEGGKITVLDTVPG
                     AWSLGFFYESVSEYTGLGGENPGKLMGLAAHGTAADETMSTFAFEPDGYRLGLLDPDAR
                     DPEDWDEYYLTQRAWFAHLERTYRLPPNEFVHRYDPAKGRVVRDTRRDPYEYRDLAATA
                     QAALERAVFGLADSVLSRTGERSLFVAGGVGLNATLNGKLLTRSTVDHVFVPPVASDIG
                     VSLGAAAVVAVELGDRIAPMGDTAAWGPEFSPDHVRAALDRTGLSYREPANLEREVATL
                     VADGKVVGWAQGRAEVGPRALGQRSLLGSAHSPTMRDHINLRVKDREWWRPFAPSMLRS
                     VSDQVLEVDADFPYMIMTTKVRAAYAERLPSVVHEDWSTRPQTVTEAGNPRYHRMLTEL
                     GDLVGDPVCLNTSFNDRGEPIVSSPADALLTFARLPIDALAVGPYLVTRDLRH"
     gene            9848..11122
                     /gene="alloG"
     CDS             9848..11122
                     /codon_start=1
                     /function="tobramycin biosynthesis"
                     /gene="alloG"
                     /gene_functions="biosynthetic (rule-based-clusters) 2dos:
                     DegT_DnrJ_EryC1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1056:DegT/DnrJ/EryC1/StrS aminotransferase (Score:
                     385.1; E-value: 6.1e-117)"
                     /gene_kind="biosynthetic"
                     /product="L-glutamine:2-deoxy-scyllo-inosose
                     aminotransferase"
                     /protein_id="BAE98081.1"
                     /sec_met_domain="DegT_DnrJ_EryC1 (E-value: 1.8e-106,
                     bitscore: 345.6, seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPVHLAINNGTPVRTRPWPVWPQPARGALGALERVLHSGRWAISG
                     PYRGAESAERRFARTFAAYNGVAHCVPAASGTASLMLALEACGVGVGDEVIAPGLSWVA
                     SASTIVGVNAVPVLVDIDPATLCLDPAAVEAAITPATRAIVVVHLYSAVADLDALRGVA
                     DRHGLPLIEDCAQAHGAEHRGRKVGSVGDIGTFSMQHSKVLTSGEGGAAITDSAALARR
                     MEHLRADGRCYPAAAPAPGHMELVETGELMGSNRCLSEFQAAVLVEQLRELDEQNALRR
                     RNALLLNELLAEQGLRPQDSSPGTTSRTYYVYAAALPDDALTDVPIAAVTGALTAELGF
                     PISPAYAPLHTNRLYAPASRRRFALGDEHEKRIDPGRFHLPVCERLTRRLITFHHAALL
                     GDESDVRDIATAVAKVLRHRGELRA"
     gene            11152..12312
                     /gene="alloH"
     CDS             11152..12312
                     /codon_start=1
                     /function="tobramycin biosynthesis"
                     /gene="alloH"
                     /gene_functions="biosynthetic (rule-based-clusters) 2dos:
                     DOIS"
                     /gene_functions="biosynthetic (rule-based-clusters) 2dos:
                     DHQ_synthase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1183:3-dehydroquinate synthase (Score: 364.3; E-value:
                     9.5e-111)"
                     /gene_kind="biosynthetic"
                     /product="2-deoxy-scyllo-inosose synthase"
                     /protein_id="BAE98082.1"
                     /sec_met_domain="DOIS (E-value: 8.1e-177, bitscore: 576.7,
                     seeds: 17, tool: rule-based-clusters)"
                     /sec_met_domain="DHQ_synthase (E-value: 1e-71, bitscore:
                     230.4, seeds: 76, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQTTTITMGDVHYPYRLGTGCVDAVVDRLGELGASHYLVLCDATV
                     AELYGNDLVARLNRAAGPATVLVHPAGEEHKGLGTLNTLADAALHAGVDRRGLVVALGG
                     GVTGNIAGLLAALLFRGIRLVHVPTTVVAMLDSVLSLKQAVNAQVGKNLVGTFYPPVEV
                     LADTAVLNTLPVREIRSGLCEVVKNALAIRPSMISFLAAELRPDGRYADDVLRWMIDES
                     IAAKAQVTEHDKYERREGLVLEYGHTVGHALEHASHGAVSHGAGVGLGMVAAARVSHRL
                     GHVDAGFAELHRELVSKVGVATTVPTSVPHQEITYRLGFDNKRGYQSLAADQYAMVLLE
                     GAGQPLRRDGLPLTPVPRALVDEVVRELAGVPSRVGASVATAGGAS"
     gene            12309..>12866
                     /gene="alloI"
     CDS             12309..>12866
                     /codon_start=1
                     /function="tobramycin biosynthesis"
                     /gene="alloI"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1079:oxidoreductase (Score: 183; E-value: 1.6e-55)"
                     /gene_kind="biosynthetic-additional"
                     /product="putative oxidoreductase"
                     /protein_id="BAE98083.1"
                     /transl_table=11
                     /translation="MSAPLGVAVVGAGFMGGVHAEVLAAHPRAQLVAVCDLDQRAAQEL
                     AGRFAVPTVTDDLDRVLADDRVGLVVVTTPNALHADQAARALRAGRHVLVEKPLALTPD
                     EIHGLLRLATERGLVLAHGSNFVQSPKFRRVRELVADAEAFGTPHLARVVVRNAGPDSG
                     WCLDPALAGGGVLLDLGCHAVEI"
ORIGIN
        1 gatccggcga catcgggatc agggggattt tcgtgcatgt catcggacga aggcgtactc
       61 gactcctcgc gtgtctcgtc gcgccgctgg ccgcgctgct gctgaccagc ggcgtgagcc
      121 ggcggccgac ccggccgcgg acgcgcccga ggcgttctcg ggcgccagct gggccgcgga
      181 ctggcccacc tggcagaagg acctgtccgg ctcccgctcc aaccgggcgg aacgccggat
      241 caccccggac aacgtggaga acctgcgact gaagtgggcg ttcacccacc cccagccgaa
      301 gggcctgccc gacggggtgc ggctgacggc caggagccag ccggcggtcg tgaacggggt
      361 cgcctacttc ggctcgaccg acgggaagtt ctacgcggtc aacgcccgga ccgggaagac
      421 gaagtgggag ctcgacctga acgaggtgaa ctccggctcc gggttcaccg cggtgcgcaa
      481 cagtcccgcg gtctccggcg ggaagatctt cttcggcgac tacgccggcc acctgtacgc
      541 ggtggaccag aagtccggcg agctggtgtg gtccacgaag atcgacaacc acccgcaggg
      601 cctcaccacc agctcgccga tcgtccacgg tggacgggtc tacgtcgggg tgtccagcgc
      661 ggagaacacc ggcgggatcg agacggcctg ctgcacgttc cgcgggcacg tcgacgcgtt
      721 ggacgcgaac accggcgcac tggtgtggcg gcactacacc gtgcccgagg ccaaggccgt
      781 gggcacctgg cccaacggcg cgacccggta cgagccctcc ggcggtggcg tgtggagctc
      841 gccggtgatc gacccgagca cgaggacgct ctacgtcgga accggccaga actacaccgg
      901 caaggagggt gagacggaca cggtcctggc gttggacatc gcgaacggcg aggtgcggtg
      961 gaagcgccag atgacgttcc ccgacgtctg gcggcaggtg tgcgccgacc cgagcgcgcc
     1021 gcccgggtac tgcccggggg cgaaggaggg cgcggcgctg gactacgact tcggggccat
     1081 gccgaacatc ttctccgcca acggccgcaa gctcgtcggc atcggccaga agaacgggat
     1141 gtaccacgtg ttcgacgccc acaccggcga ggtcgtctgg cagcgccagc tcggcaagcc
     1201 gtggcccaac ggcggtgtcg gcggaatcca gtggggcgcc agctacgacg ggaagcggct
     1261 ctacgtggcg acctggttcg ccgacccggg ttcgctgttc gcactggacc cggccaccgg
     1321 caggacgttg tgggagacca agcacccggc caacgggtgc agcaccggcg gcgcggccaa
     1381 gcaccccgag gtctgcgcgt tggccaacac cccggccgtg tcgaccagcc cgggcctggt
     1441 ctacgagggc agcgccgacg gcaagatgcg cgcctactcg gcggagaacg gcaaggtgct
     1501 gtgggagttc gacaccatcc gggagttcca gggcgtgaac ggcctcaccg gccacggcca
     1561 gggcatctcc ggcggcgccg tgatcgctga cggcgtgctc tacgtcaact ccggctactt
     1621 cggacagtac ggcaggttct acccgaccga caagggaagc gtgctcctgg cgttcagcct
     1681 gcgctgatct ccactcgtga gaggctcccc gtccccaggg acggggagcc ttttcgcgtt
     1741 cccgggcgag gcgcggcgca tcggaccgac cacatcgcac tatccgaaaa ggacactcat
     1801 gagggtgggc gggacggtgt ccggaccgcg gccggagccg ttcggagctg gttcgctcgt
     1861 tgatcaccat gttcacggag gtgcgggagg aggcggatgc gctgtcggcg cggtcgtccc
     1921 ggccgtctgc cggcggttcc ggcccgtgcg agctccaccg ccgacggccg gcggggcggg
     1981 tggaaaatct gtcagctctt cccggccagc ccgaattgcc tcgcgggcga atgtgtttcc
     2041 tgcggtgttc gcccggtcgc gggtcggagg aaagaagtgg gtcggctttc tttcatcccc
     2101 cgaaattcgt cggggcttgg gaattccgct cggccgtgcc cggcgacgcc gtccggagca
     2161 agctgtcccg cgtgtcgccg gggaccggga gggccgcttc gcgttggcgg gctggcccgg
     2221 gtggccgtgg cgtggctact cccgccgcac acagggaaaa cagggtctgc gagtgctctg
     2281 cgctgccgtt ttcacggaag cgaacggttg tcgcggccca acgggggtgg gtgcgttcgg
     2341 gtcagcgggt tcgtgcggag agctggtgaa ggccgcgccc ccgttcggtg aacccgctgg
     2401 taaacacccg tcacccagtt ctgtcgccgt ggtcccgatg gcgatcactc ggacggctct
     2461 ggtgactcga actcccgcgc cggtagcgtc tgcgctggtc ggaaatacgg tggaaaaagc
     2521 ggatgtttgg cgacgcgacc ctgcccctcg ctcccgtcgc cacgcgtcct ttggtgtgca
     2581 cgccaggact tgggggtcgt cgcgtgaagg cactgacgtt cgtagcgccc ggcgaggcgg
     2641 tcttcggcac ccgcgcggtg cccgtccccg cccccggtga ggcgctggtc cgcctcgggt
     2701 acaactcgat ctgcggcagc gacctgtcgc tgtaccgggg cgtctggcac ggattcggct
     2761 acccggtcgt gccgggtcac gagtggagcg ggacggtcgt cgaggtcaac gggcccggcg
     2821 acgagttggt cggccgggac gtggtcggcg acctcacgtg cccgtgcggc gcgtgtgccg
     2881 cctgtcagcg cgggactccg gtgctctgcg agaacctcca ggaactcggg ttcacccggg
     2941 acggcgcgtg cgcggagtac atgaccatcc cgacgcgcaa cctgcaccgg ctgccggcgg
     3001 aactgtcgtt gcgggtcgcc tgccaggtgg agccggtcgc cgttgccctg cacgctctgt
     3061 ccactgtgga cgtctcgccc ggggagaagg tggccgtcct cggcgccggc gggatcggcc
     3121 tgctgttgtc acaggtggcg cgacaccggg gcgcggtgat caccaccgtc ggcgagcccg
     3181 tccccgagcg cagggcggtc gccgcccagc tcggggcggc gacggtgacg accggccgcc
     3241 ccggcgagct ggcggacctg gtggccaaac gcccggagct gacccccgac gtcgtgctgg
     3301 aggcctccgg ctacccggtc gccctgcagg aggcgatcga ggtcgtgcgc cccggcggcc
     3361 ggatcggcgt ggtgggctac cgggtcgagg agatcagccc gatggcgacc caccacgtcg
     3421 ccgtgaaggc gttgacgatc cggggctcgc tggggcccgg caaccggttc cccgacgcca
     3481 tcgacctgct cgctcggcgc gagatcaacg tcgatccgct gctcagccac gagttcgcgc
     3541 tggccgacca cgcccgcgcg ctggagctcg cgttgcgccg caccgagggc aacatccgct
     3601 cgttcttcaa cctccgcgcc tgagggcgtt cgccggtcgg caaccccccg cgtccgcggt
     3661 ggagcgtccc cgcgccgtcg acggcgctcc acccgcaccc gccgcactgg gcgtcatctc
     3721 agggagcgac ccgtgtctca gcatgccgcg tccgcagacg aaacatcggc cggggcgccc
     3781 gccgccgacc cgagccccga atcccgggcg cgggccaggg cgacgggccg gcgactgcgc
     3841 ctgttcttcg ggctgcacga gctggtcccg ctcttcccgc tctacccgct gctgttcgcc
     3901 gaccacgggc tgtccaccgc ggggatctcc gggttgctgg ccctctggtc ggtcacggcc
     3961 ttcgtcctgg aggtgccctc cggggtcctg ggcgacatgt tctcccgccg ttggctgctc
     4021 gccgtcggca ccgccgtgcg ggcctgcggt ttcggcctgt gggtggcttt cccgtcgccg
     4081 tgggccttcg ccgccgggtt cctgtgctgg ggcctgtgcg acgcgctgac ctcgggaacg
     4141 ctggagtcgc tgatctacga cgagctgcgc gcggccggcg cgtcccaccg gtacgcggcg
     4201 gtgatgggca gcggccgggc ggtgtccctg ctctccacgc tcaccgcgat gacgttggcc
     4261 gggccggtct tcgcgctcgg cggctacccg ctcgtcggga tgatcagcct gggcagcggg
     4321 ctggcctgcg ccgtcgccgc gctgcggttc tccgagccgc cccgggacca ggtcgggcgg
     4381 gaggacggcg ggttgcgttg gtacgtcgcg accttccggg ccggggtgtc ggaggtgcgg
     4441 cgggaccggg tggtgctgcg cgcggtgctc atcgccgcgt cgctgcacag catgacggcc
     4501 atcgacgagt acttccccat gctggcaagg gcgaacggcg cgtcgacgac gatggttccg
     4561 gtgctggtgg ccgtgacggt cgtctgcgcg gcggccggca gcggtctggt cggcaggacc
     4621 gcgcacgccc cgcccgccgt gctgggcctc gccctgctgg cctcggccgg gctgatggtg
     4681 gcgggcgcgc tgagcgggct ccaggcgggc atgctcgccg tcggggtgag cttcggcctg
     4741 gtgcagtggg cgctggtgct ggccgacacg cggttgcagg acgcgatcac cggtccggcg
     4801 cgcgcgaccg tcacctcggt cgtcgggttc ggcgccgagg tggcctcggt cggcttctac
     4861 ctgggcgccg gcgctctctc caccgtcacc tccacgtccg agctgttggc ctggttcgcg
     4921 gtgccgctcg tcctcatcgc ggtggccgcc ccgcgctggt tgagacggga accgcagccg
     4981 cccggcgagg ttccccgggc gccgggagcg gaaccaccgc ggcctgcctg agcaccgagc
     5041 ggcgcgagcg ggtccacgcc tggctcggcg ccgccggccg agtccgcgac caaccccttc
     5101 tgtcctctgt ggagagtccg ctgatcgcga ggcgcgccgc caccggcgcg acctcctgcc
     5161 ggaagtccag tgcacgtgac cgttcctgac gcgtgaggag cacgtcgatg acccagacca
     5221 acgagatcag cgcgacgacg ggggactgga gccggtccgc cgagcacctg cgccggtccc
     5281 acgaggtcac caccaacccc cgtcccgacc aggacggcca gttcccctgc gtgctctcca
     5341 ggggcgcggg ggcgcgcgtg tacgacctgg acgacaacgc ctacttcgac ctcacctgct
     5401 cgttcggcac cgtcctcatc ggacacgccg agccggccgt caccgacgcg atccgggcga
     5461 gcctggacga cgggaacctg ttctacaccg ggccgagccc gcgccggctc gcgctggccg
     5521 aacgcctgct cgactggttc ccctgggccg accaggcgtt gttctaccgg accgggtcct
     5581 gcgcggtctc cgcggtcgcc cggctcaccc agcacgtcac cggccggacg accgtgctca
     5641 cctcgggcta ccacggctgg catgactggc acctggaggc ggtgccggag gccaagctgt
     5701 tcccgtccta cgccaccgag ttccacgagg acctcgacgt ctaccgcgcc tacctggagc
     5761 ggcacgcgga cgagatcgcc gccgtggtcg tcacgccgga gcccacgcgg aaaccggtgg
     5821 gccactaccg cgttctgcgc gagctggccg ccgagcacgg ctgtctgttc gtggtggacg
     5881 aggtgaagac gggcatgcgc gcggggaagg gcgggctcag cgccctcgcc gggctggaac
     5941 cggacgcggt gaccgtctcc aagggactgg ccaacgggca cagcatctcc gccgtggtgg
     6001 gcagccgccg gatcaccgac ggcctcgacg aggcccacgt gtggtccacg taccagaacg
     6061 agcaggtggg cttcgccgcc gcactggcca ctgtggactt cctggagagc caggacgtgg
     6121 ccgcggtcgt cggacgcacg ggacgcgtgg tggagcaggc gttccggagc gcgttcgccg
     6181 aacggggcct cccggtggag gtgcgcggct ggggcccgat gttcgacctc gacttctccg
     6241 ctgccgagga gggactggcc gaccgcctgc aactcgcctt cctccgccac ggcgtcttct
     6301 gcgacatcgg cgacgacttc aacgtcatgt accgcaccgc cgaccacctc gacgagctga
     6361 ccgggcgggt gaccgcggcg atcaggtcgg tctgagccgc caccgggacg gcgcccggac
     6421 cagaacccgg gccagaaggg agcgcgcatg ctgctgatct caccggacga ggccgcgcgt
     6481 cggacctacg acgcctgtgt catcggcagc ggcgcctccg gctccgtggc cgcggccgtg
     6541 ctcgccgagc gcggtctgtc ggtgctcgtg gtggaacagg gcggcccgat cccgccgggc
     6601 gccgacgggg aggaccacga ggaccccgac gggtgggcgt tcgcgcgccg cgacgggcgc
     6661 tggacccggg acggctaccc gtggacggcc atggcgctcg gcgggggaac cctgttctac
     6721 gccgggatgt cctaccgcta ccagcagacc gacctggccc cgccctcgtc gttcctcggc
     6781 gccgccgact acgaccactg gcaggtcggc ctcgccgacc tcgaaccgca ctacgagtgg
     6841 atcgagtacc agctcgcgat cgccggcccc tccaggaaac aggtgggcga ctacgtcttc
     6901 cccgtctacc accggccgag cctgccgctc acgccgcccg gcgcggcgct ggccgagggc
     6961 gctcgctcgc tccacctcac gcccctgccc accccggtgg cgatcagcgg ggtgcgggac
     7021 cggttcggcc ccggctgcgc ccagaccacc ccgtgcacct cgttctcctg tcccacgaag
     7081 gccaaggccg acgtcgtcag ccgggttctc gctcccgccg agggagacgt ctcggtggtc
     7141 gtccacacca gagtcgtccg gctcgtggcg tcgagtccgc accgggttga cgcggtcgag
     7201 gtgctcgacc gcgagaacgg cacgcgcctg accctgcgcg cccgtcgatt cctgttggcc
     7261 gccaacgcga tccagtccgc cgcgctgctg ctccggtcga caacccgcac cgagccggac
     7321 gggatcggca accggcacgg gctcgtcggg cggcacctcg cgatgaagaa cagcgtgtac
     7381 gtgcgcggga ccatcccgcg cagactgccc gggtacaccc cgttgcgaca ccggtactcc
     7441 agcgtctgcg tgttggactt cctcaccgga gaagcgtttc ccgacggaat cggcggactg
     7501 atccacgagg ccaacccgtg ggagcccgcc gccgagcggg aggcggcccg cggcaccctg
     7561 ttgcaggtcg agtgcctggt gggcgaccgg ccccaggccg agaaccgggt tcggctgtcc
     7621 cggacccggg accgggacgg ccttccccgc atcgtcatgg actaccggcc gcaccccgcc
     7681 gacctccggc gcttggccgt gttgtccgag cgcgcccacg acatcctcac caaggccggc
     7741 gccgtcgacg tgcgcgaggt ggacaccgcc tacgagttgg gcagcgcgca cctgcacgga
     7801 accctgcgcg ccggccgaga cccccgcacg tcggtgaccg acccgttcgg ccgcctgcac
     7861 gaccacgaca acgtctggtc ggtcgacggc gccacgttcc cgttcgccgg caaccccaat
     7921 cccaccctca ccatccaggc caacgcccac cggatcgcgc tcgcgatcga ctgaccgggc
     7981 gagggccgca cccaagatcc ccctgtgcca ccggaggtgg agacatgcgt gtcctcggac
     8041 tgaacggctg gccccgcgac ttccacgacg cgtcggcggc cctgctcgtc gacggccgga
     8101 tcacggcgtt cgccgaggag gagcggttca ccaggaagaa gcacgggtac gacacggctc
     8161 ccgcgcaggc ggcggcgttc tgcctggccc aggcgggcct gaccgtggac gacctggacg
     8221 ccgtggcctt cggctgggac ctgcccagga tgtaccgcga gcggctgggc ggctggccgc
     8281 actcggacag cgaggcgctg gacatcctgt tgccccggca cctcttcccc cggcgcaccg
     8341 acccgccgct gcacttcgtc cagcaccacg tggcgcacgc ggccagcgcc taccacttct
     8401 ccggcgtcga ccacggcgcg gtgctgatcg tggacggcga gggcgaggag gagtgcgtca
     8461 ccctggcccg ggccgagggc gggaagatca ccgtgctgga caccgtcccc ggtgcgtggt
     8521 ccctgggctt cttctacgag tcggtcagcg agtacaccgg cctgggcggc gagaaccccg
     8581 gcaagctcat gggactggcc gcgcacggga ccgcggcgga cgagacgatg tccaccttcg
     8641 ccttcgagcc ggacggttac cggctcggcc tgctcgaccc ggacgcgcgg gacccggagg
     8701 actgggacga gtactacctg acgcagcggg cgtggttcgc gcacctggag cggacgtatc
     8761 gcttgccccc caacgagttc gtccaccgct acgaccccgc caaggggcgg gtcgtgcggg
     8821 acacgcgacg ggacccctac gagtaccggg atctggcggc caccgcccag gcggcgttgg
     8881 agcgcgcggt gttcgggctg gccgactcgg tgctctcccg cacgggagag cgcagtctgt
     8941 tcgtcgcagg cggtgtcggt ctcaacgcga ccctgaacgg caagctgctg acgcggtcca
     9001 ctgtggacca cgtgttcgtt cccccggtgg ccagcgacat cggcgtgagc ctcggcgcgg
     9061 cggcggtggt cgccgtcgag ctcggcgacc gcatcgcgcc catgggcgac accgccgcct
     9121 ggggaccgga gttctccccg gaccacgtgc gggccgcgct cgaccgcacc ggcctgtcct
     9181 accgggagcc ggccaacctg gagcgggagg tcgccacgct cgtcgccgac ggcaaggtgg
     9241 tgggctgggc gcagggcagg gccgaggtcg gcccgagggc gttgggccag cgcagcctgc
     9301 tgggctccgc gcactccccg accatgcgcg accacatcaa cctgcgggtc aaggaccggg
     9361 agtggtggcg tccgttcgcc cccagcatgc tgcgctcggt cagcgaccag gtgctggagg
     9421 tcgacgccga cttcccctac atgatcatga cgacgaaggt gcgcgcggcc tacgccgagc
     9481 gcctgccgtc ggtggtgcac gaggactggt cgacccgccc gcagaccgtg accgaggccg
     9541 ggaacccgcg ctaccaccgg atgctcaccg agctcggcga cctggtcggc gacccggtct
     9601 gcctcaacac ctccttcaac gaccggggcg aacccatcgt ctcgtcgccg gccgacgccc
     9661 tgctcacctt cgcccgcctg ccgatcgacg ccctggccgt cggcccgtac ctggtgacca
     9721 gggacctccg ccactagcca gggcccgtcc gtccacagtg gacatcccga cgccggtcac
     9781 cgaagtgccc ctccggtcga ccaccgcggc cgcccccgca cgcccgcaga ggaagggatc
     9841 gtcgaccatg cccgtccacc tcgcgatcaa caacggaacc ccggtgcgca cccggccctg
     9901 gccggtctgg ccccagccgg cccggggcgc gctcggcgcg ctggaacgcg tgctccactc
     9961 cggacgctgg gcgatcagcg gcccctaccg gggcgccgag tcggccgagc gccgcttcgc
    10021 caggaccttc gccgcctaca acggggtcgc gcactgcgtc cccgcggcca gcggcaccgc
    10081 cagcctcatg ctcgcgctgg aagcctgcgg cgttggcgtc ggcgacgagg tcatcgcccc
    10141 cggactgtcc tgggtggcct cggcctccac catcgtcggc gtcaacgccg tcccggtcct
    10201 cgtcgacatc gaccccgcga ccctgtgcct ggacccggcc gcggtcgagg cggcgatcac
    10261 gcctgccacc agggcgatcg tcgtcgtgca cctgtactcc gcggtggccg acctggacgc
    10321 gctgcgcggc gtcgccgacc ggcacggcct gccgctgatc gaggactgcg cccaggccca
    10381 cggcgccgaa caccgcggcc gtaaggtcgg ctcggtcggc gacatcggga cgttcagcat
    10441 gcagcacagc aaggtgctca ccagcgggga gggcggagcc gcgatcaccg actccgccgc
    10501 gctggcccgc aggatggaac acctgcgcgc cgacggccgc tgctatcccg ccgccgcgcc
    10561 cgcgcccgga cacatggaac tggtcgagac cggggagctg atgggcagca accggtgcct
    10621 gtcggagttc caggccgccg tgctcgtcga gcagctcagg gaactcgacg agcagaacgc
    10681 cctgcgccgt cgcaacgccc tgctgctcaa cgagcttctc gcagagcagg ggctgcggcc
    10741 gcaggacagc tcgccaggca ccaccagccg cacgtactac gtctacgccg ccgccctgcc
    10801 cgacgacgcg ctgaccgacg tcccgatcgc cgccgtgacc ggggcgctca ccgccgagct
    10861 gggcttcccg atctcaccgg cctacgcgcc actgcacacc aaccggctct acgcgccggc
    10921 ctcccgccgc cggttcgcgc tcggcgacga gcacgagaag cggatcgacc ccggccggtt
    10981 ccacctgccg gtgtgcgagc ggctcacccg ccgcctgatc accttccacc acgccgcgtt
    11041 gctcggcgac gagtccgacg tgcgcgacat cgccaccgcc gtcgccaagg tgctgcggca
    11101 ccgcggggag ctgcgggctt gagcgcccgc accggacctt ggggagtgca gatgcagacc
    11161 accaccatca ccatgggaga cgtccactac ccgtaccggc tcggcaccgg ctgcgtcgac
    11221 gcggtcgtcg accggctggg cgagctgggc gccagccact acctcgtgct gtgcgacgcg
    11281 accgtcgccg agctgtacgg gaatgacctc gtcgcacgcc tgaaccgcgc ggccgggccg
    11341 gccaccgtcc tggtgcaccc ggcgggggag gagcacaagg ggctcggcac gctgaacacc
    11401 ctcgccgacg ccgctctcca cgccggagtc gaccggcggg gactggtcgt cgcgctgggc
    11461 ggcggtgtca ccgggaacat cgccggtctg ctcgccgccc tgctgttccg cgggattcgc
    11521 ctggtgcacg tgccgaccac ggtcgtggcg atgctcgact cggtgctgtc gttgaaacag
    11581 gccgtcaacg cccaggtggg caagaacctc gtcggcacct tctacccgcc ggtcgaggtg
    11641 ctcgccgaca ccgcggtgct gaacaccctg ccggtcaggg agatccggtc ggggctgtgc
    11701 gaggtcgtga agaacgcgtt ggcgatccgg cccagcatga tctccttcct ggccgccgag
    11761 ctgcgacccg acggccgcta cgccgacgac gtcctgcgct ggatgatcga cgagagcatc
    11821 gcggcgaagg cccaggtcac cgagcacgac aagtacgagc ggcgggaggg cctggtcctg
    11881 gagtacggcc acaccgtcgg ccacgccctg gaacacgcct cgcacggcgc ggtgtcgcac
    11941 ggcgccggcg tcgggctggg aatggtcgcc gccgcccggg tttcgcaccg cctcgggcac
    12001 gtcgacgccg gtttcgcgga gctgcaccgg gagttggtga gcaaggtcgg cgtcgcgacg
    12061 accgtgccga ccagcgttcc gcaccaggag atcacctacc ggctcggctt cgacaacaag
    12121 cgggggtacc agtccctcgc cgccgaccag tacgcgatgg tcctgctgga gggcgctggc
    12181 caaccgctgc gccgcgacgg cctgccactg acaccggtgc ccagagccct ggtcgacgag
    12241 gtcgtgcggg aactggccgg tgtcccgtcg cgcgtcggcg cgtccgtcgc caccgcgggg
    12301 ggtgcgtcgt gagcgcgccg ctgggagtcg ccgtggtggg cgccgggttc atgggcggtg
    12361 tccacgccga agtgctcgcc gcgcacccgc gtgcccagct cgtcgcggtc tgcgacctcg
    12421 accaacgggc cgcccaggag ttggccgggc ggttcgccgt tccgacggtc accgacgacc
    12481 tcgaccgcgt cctggccgac gaccgggtcg gcctggtggt ggtgacgacc ccgaacgcgc
    12541 tgcacgccga ccaggcggcc cgggcgttgc gggccggccg acacgtgttg gtcgagaaac
    12601 ccctcgccct caccccggac gagatccacg ggctgctgcg gctggccacc gagcgcggcc
    12661 tggtgctggc gcacggcagc aacttcgtgc agagccccaa gttccgccgg gttcgggaac
    12721 tggtcgccga cgcggaggcg ttcggcacgc cccacctggc ccgggtcgtc gtgcgcaacg
    12781 cgggaccgga ctccgggtgg tgcctggacc cggcgctcgc cggtggggga gtgctgctcg
    12841 atctcggttg ccacgccgtg gagatc
//
