Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0000722.1:
Cluster 1Other145164

BGC0000722, validamycin biosynthetic gene cluster from Streptomyces hygroscopicus. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: validamycin A
SMILES string: Copy to clipboard
Molecular formula: C20H35NO13
Exact molecular mass: 498.21 Da ([M+H]+)
Molecular activity: Antifungal
Molecular target: Trehalose synthase

Class-specific details

Biosynthetic class(es):
Other
Other biosynthetic class:
Cyclitol

Gene cluster description

validamycin (BGC0000722). Gene Cluster 1. Biosynthetic class = Other. GenBank DQ164098. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Homologous known gene clusters

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Knock-out studies, Enzymatic assays, Heterologous expression, Gene expression correlated with compound production, Sequence-based prediction
MIxS-compliance:Unknown
Contact for this cluster:Linquan Bai (Shanghai Jiao Tong University)

Literature references

1. Bai L et al. (2006) Functional analysis of the validamycin biosynthetic gene cluster and engineered production of validoxylamine A. Chem Biol 13(4):387-97. doi: 10.1016/j.chembiol.2006.02.002.
2. Yu Y et al. (2005) Gene cluster responsible for validamycin biosynthesis in Streptomyces hygroscopicus subsp. jinggangensis 5008. Appl Environ Microbiol 71(9):5066-76. doi:
3. Minagawa K et al. (2007) ValC, a new type of C7-Cyclitol kinase involved in the biosynthesis of the antifungal agent validamycin A. Chembiochem 8(6):632-41. doi: 10.1002/cbic.200600528.
4. Xu H et al. (2008) Catalytic analysis of the validamycin glycosyltransferase (ValG) and enzymatic production of 4''-epi-validamycin A. J Nat Prod 71(7):1233-6. doi: 10.1021/np800185k. Epub 2008 Jun 19.
5. Xu H et al. (2009) Alternative epimerization in C(7)N-aminocyclitol biosynthesis is catalyzed by ValD, a large protein of the vicinal oxygen chelate superfamily. Chem Biol 16(5):567-76. doi: 10.1016/j.chembiol.2009.04.006.