LOCUS       BGC0000749              5120 bp    DNA     linear   BCT 06-MAY-2004
DEFINITION  Escherichia coli strain B44 K30 capsule biosynthesis gene cluster,
            partial sequence.
ACCESSION   BGC0000749
VERSION     BGC0000749
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
COMMENT     ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2024-10-17 14:08:51
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5120
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..5120
                     /db_xref="taxon:562"
                     /mol_type="genomic DNA"
                     /organism="Escherichia coli"
                     /strain="B44"
     subregion       1..5120
                     /aStool="mibig"
                     /contig_edge="True"
                     /label="saccharide"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..5120
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     misc_feature    213..226
                     /note="RcsAB box"
     gene            394..1290
                     /gene="galF"
     CDS             394..1290
                     /codon_start=1
                     /gene="galF"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) NTP_transf_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1064:glucose-1-phosphate
                     adenylyl/thymidylyltransferase (Score: 188.4; E-value:
                     2.8e-57)"
                     /gene_kind="biosynthetic-additional"
                     /note="GalF; regulatory subunit"
                     /product="UDP-glucose pyrophosphorylase"
                     /protein_id="AAN52283.1"
                     /sec_met_domain="NTP_transf_3 (E-value: 6.3e-11, bitscore:
                     30.6, seeds: 297, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNMANLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDE
                     IVAAGIKEIVLVTHSSKNAVENHFDTSYELEALLEQRVKRQLLAEVQAICPPGVTIMNV
                     RQAQPLGLGHSILCARPVVGDNPFVVVLPDIILDGGTADPLRYNLAAMVARFNETGRSQ
                     VLAKRMPGDLSEYSVIQTKEPMVAEGQVARIVEFIEKPDEPQTLDSDLMAVGRYVLSAD
                     IWAELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGESYDCGKKMGYMQAFVTYGMR
                     NLKEGTKFRESIKKLLA"
     CDS             1683..1799
                     /codon_start=1
                     /note="Orf2; similar to Klebsiella ORF2 encoded by GenBank
                     Accession Number D21242"
                     /product="unknown"
                     /protein_id="AAN52284.1"
                     /transl_table=11
                     /translation="MNWQLISFFGDSTVLLPNSAALFIVLMLCKTSRLLAWQ"
     misc_feature    3267..3305
                     /note="JUMPstart element"
     CDS             3671..5110
                     /codon_start=1
                     /function="involved in assembly of the K30 capsular
                     polysaccharide"
                     /note="OrfX"
                     /product="unknown"
                     /protein_id="AAN52285.1"
                     /transl_table=11
                     /translation="MIKIARIAVTLGLFSSLGAQAYAAGLVVNDNDLRNDLAWLSDRGV
                     IHLSLSTWPLSQEEIARALKKAKPSYSSEQVVLARINQRLSALKADFRVTGYTSTDQPG
                     TPQGFGQTQPADNSLGLAFNNSGEWWDVHLQGNVEGGERISNGSRFNANGAYGAVKFWN
                     QWLSFGQVPQWWGPGYEGSLIRGDAMRPMTGFLMQRAEQAAPETWWLRWVGPWQYQISA
                     SQMNQYNAVPHAKIIGGRFTFSPIQSLELGASRIMQWGGKGRPESLSNFWDGLTGKDNT
                     AANDPNEPGNQLAGFDFKFKLEPTLGWPVSFYGQMIGEDESGFLPSANMFLGGIEGHHG
                     WGKDAVNWYLEAHDTRTNMSRTNYSYTHHIYKDGYYQQGYPLGDAMGGDGQLVAGKVEL
                     ITEDNQRWSTRLVYAKVNPENQSINKAFPHADTLKGIQLGWSGDVYQSVRLNTSLWYTN
                     ANNSDSDDVGASAGIEIPFSL"
ORIGIN
        1 aatcgcagtt ccccaccgcg gattcaggct aatatgacgt catatccggc tgtttatact
       61 gaagtatatt gcctggcgaa atttattatc tatatatatt cttttcgggc atacgcgcgg
      121 cgggtccggc gtccaacagg ccccgataga cgggcatctc agccacatcg tgcggagcaa
      181 aggaacgagc cgctgaataa cctgaacgag aataagatta ttctcagttc taactgttgg
      241 taaaagggcg ttttgtgtat tcttttacgt gacagtgaat ttaagaattt ggcgggcata
      301 tgcccgggca gtatccgtgt attatcagtt tcaatggatt tgtgacatgt acaattttgc
      361 aaaaccgcgt gttttgcaac caaacaggtg aagatgaata tggcgaattt gaaagcggtt
      421 attccggtcg caggactagg catgcatatg ctgccggcca caaaggcaat tcccaaggag
      481 atgctgccga tcgttgataa gccaatgatt cagtacattg ttgacgaaat cgtcgccgcc
      541 gggatcaaag agattgtcct ggtgacgcac tcctcgaaga atgcagtgga aaaccacttc
      601 gacacctcct atgagctgga agccctgctg gaacagcgcg tgaagcgcca gctactggcg
      661 gaagtgcagg ccatttgccc gccgggcgta accatcatga acgtgcgtca ggcacagccg
      721 ctgggtctgg gccactccat cctctgcgcc cgcccggtag tcggggacaa cccgtttgtg
      781 gtggtgctgc cggatatcat tctcgatggc ggcactgcgg acccgctgcg ctataacctc
      841 gctgccatgg tcgcccgttt taacgagacc ggccgcagcc aggtgctggc gaaacgcatg
      901 ccgggcgatc tctccgaata ctccgttatc cagaccaaag agccaatggt ggccgaaggg
      961 caggtggcgc gtatcgtcga gtttattgaa aaaccggacg aaccgcagac cctggactcc
     1021 gacctgatgg cggtgggccg ctacgtgctg tcggcagata tctgggctga acttgaacga
     1081 acccagccgg gcgcctgggg ccgtatccag ctaaccgacg ccattgccga actggcgaag
     1141 aagcagtccg ttgacgcgat gctgatgacc ggcgaaagct atgactgcgg taaaaaaatg
     1201 ggctacatgc aggccttcgt cacctacggg atgcgtaacc tgaaagaagg taccaaattc
     1261 cgcgaaagca ttaaaaaact gctggcctga gcgccggcgt aagggtgagc ggcagggcag
     1321 gggtattttg ccacccgctc ataacgttat gcagaaatta ttcataacgt gaatgttata
     1381 acgattgtaa agaaacgtat ggcttcggag acttctggta gtttgtctct ggcatctgtg
     1441 gtgcaatagt cggagtagcg gcaataatgc tttatgaata gtcacttagt gcgacaattc
     1501 ctaaagtgca tggaaagcgg tcaacgtcag ccaaaaataa aacttgactg cgatcatgtt
     1561 tccgctttaa acatctcact tgcatgcata aaaatgtgaa atgaattggc atgcgacctg
     1621 gtatagacat tacggataat ccctgagcac ggcagtggac attatcgaag gatcacaggt
     1681 atatgaactg gcaactcatc tcattttttg gcgatagcac cgttttgctg cccaactcag
     1741 cggcgctgtt tattgtcctg atgctgtgta aaacgtcgcg gttgctggcg tggcagtgaa
     1801 gtttgctgtt cggcattgct ggcgttatcg tctgcgcccc caaactggcc tttatgggct
     1861 gggtcatttg tgagctggat tacaccggct tcagcggcct tctggcctat cttcctgtgg
     1921 tactcagctc ccgtttctcc gtcggtctgc ataaagcggc cattattacc gtctatgttc
     1981 tggccgctgt ggtggctatt cgcggctggt catccatgtg cattccgtct cggaagtggt
     2041 tgccggcccg ctgctggcaa cgctttgttc ctgctgctgc aaaaacgtac ctctgatccg
     2101 gaaagcgtga atatctcatg gggcggtgtc gcttgcctgg tgatggttcc gcttatcctg
     2161 ctacatagcg gcagcaaagc accaactcag tccctgcggg gacaaatcgc caccgcggtg
     2221 gggccgctgg ataacccttt acgcgtaccg atctgcacaa gcatgcttgg taattgccat
     2281 ttgttttaca ggggaaatta agttttcgcc ggttaattag acaaataaga aaataatcta
     2341 ctatatactc ggtaattgat aattcatatt tatgaaataa tgggtggtgg ctccaactta
     2401 ctgatagtgt ttgatgttca gataatgccc gatgaccttt tcatgcagtt ccaccgattt
     2461 tgagaacgac agtgacttcc ttcccagccg ggctagatgc tgcctcagat tcaggttatg
     2521 ccgctcaatg cgctgcgtgt aacgcttgct gataacgtgc agcctctcct taagccgtga
     2581 ctcatacagc gaccatccgt ccgtcatcca tatcacgact tcaaagactg acaacaggct
     2641 cagaagacgc tccagcgtgg tcatagtgcg ttctctgaat acatgggcca caactgtcct
     2701 gcgcatcctg tcatacgcgt aaaacagcca gctctggggt gatttagcgc cgacgtagcc
     2761 ccactgttcg tccatttccg cgcagacaat gacgtcactg tccggttgta tgcgcgaggt
     2821 taccgactgc ggcctgagtt ttttaagtgc cgtaaaatcg tgttgaggcc aacgcccata
     2881 atgcatgcac tggcgcgaca tccgacgcca ttcatggcca tatcaataat tttctggtgt
     2941 gtaccgggca gagaggcggt gtaagtgaac tgcagttgcc atgttttacg gcaatgagag
     3001 cagcgatagc gctgatgtcg ggcagtgctt ttgccgttac gcaccacgcc ttcagtagct
     3061 aaacaggaag gacagctgat ggaaatggaa gccactggag cacctcaaaa aaaccatcat
     3121 acactaaatc agtaagttgg gagcaccgat attcggtaac aacactccca attgtgaccg
     3181 aaattctgta aacttagcgc gccaaaaata atggcctgac taattattca tccacaggtc
     3241 gataaaaatt aagtcgttca ggtagtcagt gcgctggtag ctgttaagcc aggagcggta
     3301 gcgtcgctga agccgcttgc tgctgcacag aggttctctt caacggctgt aaatatcgac
     3361 ccggtcagga tgatcatcat caatagatat ctatcaattt tctacggata tcctcagaag
     3421 ttggccagga gcggctgctg atatcgcctg gacattaact ttcctgtctc atcgatagcc
     3481 tgtagggtaa aattcgccta cattgtgagc agagtggatg ttataaggct aacaagcagc
     3541 ttagggtaaa tgtacttgcc tcgccggtgt tgcacagcaa agtcagctgg tgccgcgagc
     3601 gctttctatc ttggtattcc cctcattctc attgagatac gacagcgggg ctgaaaatgg
     3661 atcttgtaca atgataaaaa ttgcgcgcat tgccgtgacg ttgggtttgt tttcctccct
     3721 gggagcccag gcttacgcgg ccgggttagt ggtaaatgac aacgacttgc gtaacgacct
     3781 ggcctggctt tccgatcgcg gggtcatcca tctgagcctg tcgacgtggc cgctgagcca
     3841 ggaagagatc gcccgggcgc tgaaaaaggc caaaccttcc tattcttctg agcaagtggt
     3901 gctggcgcgt attaaccagc gactgtctgc cttaaaagcg gatttccggg tcaccggcta
     3961 cacttcaacc gatcagccag gcactccgca ggggtttggt cagacgcagc cggcggataa
     4021 ttcgttaggc ctggcgttca acaatagcgg cgagtggtgg gatgttcacc tgcagggcaa
     4081 cgttgaaggg ggagagcgga tcagcaacgg atcgcgcttc aacgctaacg gcgcctacgg
     4141 cgcggtgaag ttctggaacc agtggctctc ttttggtcag gttccgcagt ggtggggacc
     4201 tggctatgaa ggtagcctga tccgcgggga tgcgatgcgg ccgatgaccg gtttcctgat
     4261 gcagcgagcc gagcaggcgg cgccggaaac ctggtggtta cgctgggtgg ggccatggca
     4321 gtaccagata tccgccagcc agatgaatca atataacgcg gtaccccatg ccaaaattat
     4381 cggcggtcgt ttcactttct cacctatcca gtcactggaa ttaggtgcgt cgcgtattat
     4441 gcagtggggt ggaaaagggc ggcctgaatc actcagtaac ttctgggatg gtttaacagg
     4501 caaggataat accgccgcaa acgatcccaa tgaaccaggg aaccaattgg ccggttttga
     4561 ctttaagttc aaattggagc cgaccctggg ctggccggtg agcttctacg ggcagatgat
     4621 cggcgaagat gagtctggtt tcctgccttc agcaaatatg ttcctcggcg ggattgaagg
     4681 gcaccacggc tggggtaagg atgcggttaa ctggtacctg gaagcgcatg atactcgtac
     4741 caatatgagc cggaccaatt acagttatac gcatcatatc tataaggatg gctactatca
     4801 gcagggttat cctctgggtg acgcaatggg cggggatggt caactcgttg ccggtaaggt
     4861 tgagcttatt acggaggata accagcgctg gagtacacgc ttagtatacg cgaaggtcaa
     4921 cccggagaat cagtcgatca acaaagcatt ccctcatgcg gatactctta aaggtataca
     4981 gcttggctgg agcggggatg tttatcagtc ggttcgtttg aatacttcac tgtggtacac
     5041 caacgctaat aacagcgaca gcgacgacgt tggggccagc gcagggattg aaataccgtt
     5101 tagtttataa ggctgaatgc
//
