LOCUS       BGC0000751              6810 bp    DNA     linear   BCT 26-JUL-2016
DEFINITION  Actinobacillus pleuropneumoniae serotype 12 capsular polysaccharide
            biosynthesis gene cluster, partial sequence; and unknown gene.
ACCESSION   BGC0000751
VERSION     BGC0000751
KEYWORDS    .
SOURCE      Actinobacillus pleuropneumoniae
  ORGANISM  Actinobacillus pleuropneumoniae
            Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Actinobacillus.
COMMENT     ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2022-11-07 20:28:39
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6810
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     gene            complement(<1..416)
                     /gene="cps12D"
     CDS             complement(<1..416)
                     /codon_start=1
                     /gene="cps12D"
                     /gene_functions="transport (smcogs)
                     SMCOG1186:polysaccharide export protein (Score: 56.8;
                     E-value: 2.5e-17)"
                     /gene_kind="transport"
                     /note="involved in export of capsular polysaccharide to
                     outer membrane of the cell"
                     /product="Cpx12D"
                     /protein_id="AAS77489.1"
                     /transl_table=11
                     /translation="MKLIKLRLLLSLGLVASLAACSSLPTSGPSHTAVLEANSRNSDKP
                     LPEVNLVELDNGLVQQLYQTQQSQQFSGFLGTVGSAGYAGAVNVGDVLEISIWEAPPAV
                     LFGGTFSSEGQGSGHLTQLPAQMVNQNGTVTVPFV"
     source          1..6810
                     /db_xref="taxon:715"
                     /mol_type="genomic DNA"
                     /organism="Actinobacillus pleuropneumoniae"
                     /serotype="12"
                     /strain="8329"
     subregion       1..6810
                     /aStool="mibig"
                     /contig_edge="True"
                     /label="Saccharide"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..6810
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            650..1765
                     /gene="cps12A"
     CDS             650..1765
                     /codon_start=1
                     /gene="cps12A"
                     /note="involved in synthesis of capsular polysaccharide"
                     /product="Cps12A"
                     /protein_id="AAS77490.1"
                     /transl_table=11
                     /translation="MNKMNRKFSKLLKNPHIFFRDFLNKKYPIKNTELPFSESEEANLI
                     EANQKLDKIIQKNTLQQANIDVVFTWVDGSDPSWQAKYSQYAPNYQAKSALYATDIARF
                     EDHNELYYSVHAVLKYMPWVRHIFIITDNQKPKWLDETRQEKITLIDHQDIIDKEYLPT
                     FNSHVIEAFLHKIPNLSENFIYFNDDVFIARELQAEHFFQANGIASIFVSEKSLSKMRD
                     KGIITPTLSASEYSIRLLNKYYDTNIDSPLVHTYIPLKKSMYELAWLRYEKAILGFLPN
                     KLRTNNDLNFANFLIPWLMYFEGKAMPKIDICYYFNIRSPNAISLYKKLLLKQQMGEEP
                     NSFCANDFNSNYSIENYRNNLISTLNNYYKF"
     gene            1777..4401
                     /gene="cps12B"
     CDS             1777..4401
                     /codon_start=1
                     /gene="cps12B"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /note="involved in synthesis of capsular polysaccharide"
                     /product="Cps12B"
                     /protein_id="AAS77491.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 2.2e-27,
                     bitscore: 84.1, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNKISKRKFRKLKNTPGLFFKDFYTNRLLQLRNFIYLNSRKKKLN
                     SKKFTIISAVYNVSEYLDDYLESLVNQRLDFETSIDVILVNDGSPDDSEIIIKKWIKKY
                     PNNIHYIKKKNGGQSSARNLGLKFVKTEWVTFIDPDDFLDLNYFYLLNDTLEKYDHIGA
                     FVTKFKLFKEKFGTYHDGFQTDFCFTKPIRVLKANDMEDCVQFSSSSSVYRTDVIHKNK
                     ILFDEKLTASFEDTKFFYDYLYNIKESNILYIKDAIYNYRLRSNESSSSNSQWTKKAKY
                     QEFFQFGLLSVIKKYNENGTIPTFIQRLVLFSIIPYLQVAMINKKRIEDVLNESEINNL
                     LQSIKECLSYVETNTLEKFYNSPGNYFWINAINNYFYNKLPIDKRVYINKVNLDENKVY
                     FRFYGIKGKTKFSLKVNNKHLKTSSERVIEYKLFSDNLINEYNICYHIEPNKKIEFLLD
                     GEKAKIYTDFKILSDKDTDFYKGYISKNNSLKNIALFIDSGYKADDNAEHLYEKLLKNK
                     NLDNFIDDHYYLLDKESEHWNRLILKGFNLVDIKSMKGVWLMKNAKYIFCSYLPGHLNE
                     WATHHSFKFQKFIFLQHGIITSNLSKPFNASYSQIYKMVISSKFEKSEILDDKFNYIFH
                     SNDLILSTIPRLDKLVNHKRNQSNKVKKILVCPTWRTSLGNINFNKKDAISSFKETSYI
                     KNWLGLLYSDKLRNYLEEGKIEISFLPHQNFHQLLEENSLNEKLFFDINENIRILNPKK
                     SSYQELFIDHDILITDFSSLHFDFATLQKDILYFQFDKDEFYGISHAYQKGLFNFEKDG
                     FGQVTYTLEELLDQLVILINSQNEKVVNGYKKRISNVFLPSLGDSCNYILKNVFTNPKR
                     NIN"
     CDS             5356..5961
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Orn_Arg_deC_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1264:diaminopimelate decarboxylase (Score: 167.9;
                     E-value: 4e-51)"
                     /gene_kind="biosynthetic-additional"
                     /product="unknown"
                     /protein_id="AAS77492.1"
                     /sec_met_domain="Orn_Arg_deC_N (E-value: 4.1e-48, bitscore:
                     151.9, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNHFNYKNQQLFAEDVSVSDIINQYGTPAYIYSRATLERHWHAFD
                     KAFGAHPHLICFAVKSNSNIALLNVMARLGSGFDIVSQGELERVLAAGGKPSKVVFSGV
                     AKSHSEIQRALEVGIRCFNIESIAELHRINEVAGQLGKIAPISLRVNPDVDAHTHPYIS
                     TGLKENKFGVSVTNEGNKYWEKRYEKKHRRKTYCVKVR"
ORIGIN
        1 acgaacggca cggtaaccgt accgttttga ttaaccattt gtgccggtaa ttgcgttaaa
       61 tgcccgctac cttgtccctc agaactaaaa gtaccgccaa acagcaccgc cggcggagct
      121 tcccaaattg atatttcaag tacatcaccc acattgaccg caccggcata gcccgcgctg
      181 cctactgtgc ctaaaaatcc ggaaaattgt tggctttgct gagtttgata caactgttga
      241 actaaaccgt tatctaattc cactaaattg acttccggta acggtttatc cgaatttcgg
      301 gagttagctt ctaaaaccgc agtatggcta gggcctgagg tcggaagact tgagcaggca
      361 gccaaactag caaccagccc caaagaaagg agtaatctaa gtttgatgag tttcatctaa
      421 tttctcttca atatattaag gaataacaac tatataggta tgtcttaaaa tctatataaa
      481 gattgatttt aataagttac ctaatcaaga gaaattaaat ataaaaaatt tacaaaaaag
      541 caataatgcg tataaaaaaa catcatttgc aaagaaagta aatagagagg agagttcaat
      601 agataagcat tataatccaa gatttatata aaatataatt gataatataa tgaacaaaat
      661 gaatagaaaa ttttctaagt tactaaaaaa tccacatatt ttttttaggg attttctaaa
      721 taaaaagtac cctataaaaa atacggaact tcccttctca gaatctgaag aagctaactt
      781 aatagaagca aaccaaaaat tagataagat tatccaaaag aatacgttgc aacaagctaa
      841 tattgatgtg gtatttactt gggtagatgg ttctgatcct tcatggcaag ctaaatattc
      901 ccaatatgca ccaaattatc aagcgaaatc cgctctatat gcaacggata tcgcccgatt
      961 tgaagatcat aatgaattat attattcagt acatgctgta cttaaatata tgccttgggt
     1021 taggcatata tttattataa cagataatca aaagccaaag tggttggatg agacgagaca
     1081 agaaaaaatt acactaatcg atcatcaaga tattatagat aaagaatatc ttccaacgtt
     1141 taattcccat gttattgaag catttttaca taaaattcct aatttaagcg agaattttat
     1201 ctattttaat gatgatgttt ttattgctcg agaattacaa gctgaacact tttttcaagc
     1261 aaatggaata gcctctattt ttgtgtcgga aaaaagtctc tctaaaatgc gtgataaagg
     1321 tattataacg ccgactcttt ctgcttcgga atatagtatt cgcttattaa acaaatatta
     1381 cgatacaaat attgactcac ctctcgtgca cacttatatc ccattgaaaa aaagtatgta
     1441 tgaattggca tggctgcgtt atgagaaagc aattcttgga tttttaccca ataaattaag
     1501 aacaaataac gatttaaatt ttgcaaactt tcttattcct tggttaatgt atttcgaagg
     1561 gaaagcaatg cctaaaatag atatttgtta ttattttaat attagatctc cgaatgcaat
     1621 ttcactatac aaaaaactac tacttaaaca acagatggga gaagaaccta actcattttg
     1681 tgcaaacgat tttaacagta attattctat tgaaaattat agaaataatc ttatttccac
     1741 tttaaataac tattataaat tttgagaaca tttaacatga ataaaattag taaaagaaaa
     1801 tttagaaaat taaaaaacac tccagggtta ttttttaagg atttttatac aaacaggtta
     1861 ttacaattaa gaaacttcat atatttaaat agtcgaaaga aaaaactaaa ttctaaaaaa
     1921 tttactatta tcagtgctgt ttataatgtg tcagaatatt tggatgatta cttagaatca
     1981 ttagtaaatc aaagactaga ttttgaaaca agtatagatg ttatcttagt aaatgatggt
     2041 tctccagatg actctgaaat aattataaaa aaatggatta aaaaatatcc aaataacatt
     2101 cactatatta agaaaaaaaa tggaggacaa tcttctgcaa ggaatctagg attaaagttt
     2161 gtgaaaactg aatgggtaac atttattgat ccagatgact tcctagactt aaattatttt
     2221 tatttattaa atgacacctt agaaaaatat gatcatatcg gggcatttgt tactaaattt
     2281 aaactattta aagaaaaatt cggaacatat catgatggat tccaaaccga tttttgcttt
     2341 acaaaaccaa taagagtgtt aaaagcaaat gatatggaag attgtgtaca attttcatcc
     2401 agctcatctg tttatagaac agacgttatt cacaaaaata aaatcttatt tgatgaaaaa
     2461 ttaactgctt cttttgaaga tacaaagttt ttctatgatt atttatataa tattaaagaa
     2521 tcaaacattc tgtatataaa agatgcaata tataattaca gattaagatc aaatgagtca
     2581 tcttcatcca atagccaatg gacaaaaaaa gctaaatacc aagagttttt ccaattcggt
     2641 ctactatctg taattaagaa atataatgaa aatggtacta tacctacatt tattcaaaga
     2701 ttagttttat tttctataat tccatattta caagttgcga tgataaataa aaaaagaatt
     2761 gaggatgtat taaatgaatc agaaataaac aatctattac aatcgattaa agaatgttta
     2821 tcttatgttg aaactaatac tttagaaaaa ttttataact ctccgggaaa ttatttctgg
     2881 atcaacgcta taaacaatta cttttataat aaacttccta ttgataagag agtttatatc
     2941 aataaagtaa atcttgatga aaacaaagtc tactttagat tctatggaat aaaaggtaaa
     3001 acaaaatttt ctttaaaggt taataataaa cacttaaaaa caagctcaga aagagttata
     3061 gaatataagt tattttcaga caatttaatt aatgaatata atatttgtta tcatatagaa
     3121 ccaaataaga aaatagaatt cttattagat ggagagaaag caaaaatata tacagacttt
     3181 aaaatattat ctgacaaaga tactgacttt tataaaggat atataagtaa gaataattct
     3241 cttaagaata tagcattatt tattgattct ggttataaag ctgatgataa tgctgagcat
     3301 ttatacgaaa aattattaaa aaataaaaac ttagataatt ttattgatga tcactactat
     3361 ttgttagata aagaaagtga acattggaat aggttaatat tgaaaggatt taatctagta
     3421 gatataaaat ctatgaaagg agtatggtta atgaagaatg caaaatacat attttgttca
     3481 tatttaccag ggcatctgaa tgaatgggca acacatcata gctttaagtt ccaaaaattc
     3541 atatttttac aacatggtat aattacatca aatttatcaa aaccatttaa tgcatcgtat
     3601 tcacaaatat ataagatggt aatatcaagt aaatttgaga aaagtgagat attggatgat
     3661 aaatttaatt atatttttca tagtaatgat ctaattttat ctacaatccc aagattagat
     3721 aaattagtta accataagcg aaatcagagt aataaggtga agaagattct tgtttgccca
     3781 acatggagaa catcattagg taatataaac tttaacaaaa aggatgccat atcatcattt
     3841 aaagaaacaa gttatataaa gaattggtta ggcttattat actcagacaa attgagaaac
     3901 tatctagaag aagggaaaat agaaatttct ttcctaccac atcagaattt tcatcaatta
     3961 cttgaggaaa atagtttaaa tgagaaacta ttctttgata taaatgaaaa tataagaata
     4021 cttaacccga agaaatcttc ttatcaagaa ttatttattg atcatgatat attaattaca
     4081 gatttctcat cattacattt tgactttgca accttacaga aagatatttt atattttcaa
     4141 ttcgataaag atgaattcta tggaatttca catgcttacc aaaaaggatt atttaatttt
     4201 gaaaaagacg gatttggtca agtaacatac actttagagg aactattaga tcaattagtt
     4261 atactaatta attctcaaaa tgaaaaggta gttaatgggt ataaaaaaag aatatcgaat
     4321 gtatttttgc ctagcttagg tgattcttgt aactatattc ttaagaatgt atttactaat
     4381 ccaaaaagaa atataaacta aaacattaag tagtcaaagt taatagccta taaagacttt
     4441 atcattttga ctactgtttg tatctgacat tatactaaat aattttaaat accttataca
     4501 aatctttctg ttataagtaa ttttataaca tactataaaa tatactgata ctaataactt
     4561 taaccaagcg gtcaaatttt tcttaaaact tgcaaagttt aggaagaata agaccgctta
     4621 ttttattcat attcttatat aaaattagag tttattatga gcgtagccac atataattga
     4681 taaagatatg cggtatagca atattaatac aaccctatca atttcatatt atcttaatca
     4741 aatttatttt ttcgtttttt aaatattaaa ctattattta cttctgcaaa tctcccctat
     4801 cccctcttta ctaaagaggg ggatttcctt tagatattaa caagctatga tttttcgaat
     4861 agctttggtc tctcaataga ttcaggtaaa tggtcgatat ataaggaaaa taaaaacatt
     4921 cgttaaagac gagcgccatc ttagagaaat ttggagccac agcatggctc actcagccgt
     4981 aggctgatcg taaccacgta cggcttgccg tgcgtggcta ttgggtgatt atttaaaaac
     5041 aggcgaaacg gcttttttac tttttcatta ccgcaatttg ttttagaatc aacggacgat
     5101 ttactctcaa ggatttatga tgaaaaaatt atttttagcg gcgttaatcg catcgttcgg
     5161 gttggctgcc tgcggtgtaa aaggcccgct ttattttccc gagcagcaac cggctcaaca
     5221 acaaacaaaa taattgctaa ccacggtaat tcaaaatcta cgtataacaa gcggtcgaat
     5281 ttattcgatt ttttgtaaat gcgagtgtag tgagtttgcc gtggttttat tttttcaaac
     5341 acaacggaca aattaatgaa tcatttcaat tataaaaacc aacaactttt tgcggaagac
     5401 gtttctgtct ccgacatcat taatcaatac ggcacaccgg cttatatcta ttctcgcgct
     5461 acgcttgagc gtcattggca cgcttttgat aaagcattcg gcgcacaccc gcacttgatt
     5521 tgttttgcgg tgaaatctaa ctccaatatc gcgctattaa atgtaatggc acgcctcggt
     5581 tcgggttttg atattgtgtc gcaaggtgag cttgaacgtg tacttgctgc aggcggtaag
     5641 ccgagcaaag tggtattttc cggtgtggca aagtcacata gtgaaattca acgtgcattg
     5701 gaagtcggca ttcgttgttt taatatcgaa tccatcgccg agttacaccg cattaatgaa
     5761 gtcgccggtc aattaggtaa aatcgcaccg atttcattgc gtgtaaatcc ggatgtggat
     5821 gcgcacactc acccttatat ttccaccggt ttaaaagaaa ataaattcgg ggtaagcgta
     5881 acgaacgagg gaaacaagta ctgggagaag cgttacgaga aaaaacacag gcggaaaacc
     5941 tactgcgtga aagtgcgcta aattggacga ttctccgccc gtgcgggctg aatacaacag
     6001 aaggcgaaac cttccgttta attgaaaatg cggctgaact gcccggcagt tatatgagcc
     6061 gtaaagcatt agccaatgcg gtcttgtccg tacttaacag tgaaaacaca aaccataaaa
     6121 tcttctcagt ctgtgcctaa cttcacaatc cctttcactt tggcacaagc catccgcttg
     6181 tgcctatttc aaaatttgtt ttaacagttc caataaaacc ggtaattccg ttactccctc
     6241 tcgatcgaca aaatgcccgc ccgttgccaa gcgaatataa tccgcttgta agtattgcgc
     6301 taatcgatcg ctgaacgaat ggggaacgac aacatcattt aatgcagata tgacgtaaga
     6361 cttttgcggt aaacaagcgg tctgatttgc ataaaaatct gcaaagctat ctaattccgg
     6421 caaagttggt aatttctcat aaaagccgga aacaaaaatt gccgttttta ctttttgctg
     6481 cgttaccgcc agataattca gtaacgcaat gcagcccaaa ctatgtccga tgagtaaggt
     6541 attttcatct aattgaagtg tattttggtg atgttccagc catgcttgcg gattcggctg
     6601 atcggaattc ggcatcgcta aacattcaca ttcccatcct aatttttcca attcgttttt
     6661 aagccacgga aaccaatttc ttgtcgggtt cgccgtataa ccgtgcgtta catatacttt
     6721 tttcattctt tcctctgctt tatttttatt tcgaaaacgt ttgcttcacg gttaatttag
     6781 cctaaaaaat cagccttgaa aagtgagatc
//
