LOCUS       BGC0000764             16963 bp    DNA     linear   BCT 01-MAY-2007
DEFINITION  Lactococcus lactis exopolysaccharide biosynthesis gene cluster,
            complete sequence; and unknown genes.
ACCESSION   BGC0000764
VERSION     BGC0000764
KEYWORDS    .
SOURCE      Lactococcus lactis
  ORGANISM  Lactococcus lactis
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
            Lactococcus.
COMMENT     On May 1, 2007 this sequence version replaced AY741550.1.
            ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-07 20:30:53
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     misc_feature    1..455
                     /note="possible transposase fragment"
     source          1..16963
                     /db_xref="taxon:1358"
                     /mol_type="genomic DNA"
                     /organism="Lactococcus lactis"
     subregion       1..16963
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000764"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..16963
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     misc_feature    complement(724..1218)
                     /note="similar to transposase of IS1077E from Lactococcus
                     lactis IL1403"
     misc_feature    complement(1143..1517)
                     /note="truncated transposase; similar to transposase of
                     IS1077 from Lactococcus lactis IL1403"
     CDS             complement(1685..2143)
                     /codon_start=1
                     /note="Orf1; similar to IS1077-like transposase"
                     /product="unknown"
                     /protein_id="AAX19714.1"
                     /transl_table=11
                     /translation="MTKYSFELKLKVVQDYDNGVGGCDYSAKKYHVTNEAIVRRWVKAY
                     KELGAVGIQRKRQNTVYSTQFKLNAVNLYLTSEKSYGELVHEMGMNNPPLLTRWVSNYR
                     KKGEFAFSNVQGRPRKEPELLEISIKKAKDVVNETEQNLLGFKTTILI"
     regulatory      2439..2444
                     /regulatory_class="minus_35_signal"
     regulatory      2462..2467
                     /regulatory_class="minus_10_signal"
     gene            2590..2907
                     /gene="epsR"
     CDS             2590..2907
                     /codon_start=1
                     /gene="epsR"
                     /note="similar to transcription regulator"
                     /product="EpsR"
                     /protein_id="AAX19698.1"
                     /transl_table=11
                     /translation="MNDLFYHRLKELVEASGKSANQIERELGYPRNSLNNYKLGGEPSG
                     TRLIGLSEYFNVSPKYLMGIIDEPNDSSAINLFKTLTQEEKKEMFIICQKWLFLEYQIE
                     L"
     gene            2941..3708
                     /gene="epsX"
     CDS             2941..3708
                     /codon_start=1
                     /gene="epsX"
                     /product="EpsX"
                     /protein_id="AAX19699.1"
                     /transl_table=11
                     /translation="MMKKGIFVITIVISIAFIIGGFYSYNSRISNLSKADKGKEVVKNS
                     SEKNQIDLTYKKYYKNLPKSVQNKIDDISSKNKEVTLTCIWQSDSVISEQFQQNLQKYY
                     GNKFWNIKNITYNGETSEQLLAEKVENQVLATNPDVVLYEAPLFNDNQNIEATASLTSN
                     EQLITNLASAGAEVIVQPSPPIYGGVVYPVQEEQFKQSLSTKYPYIDYWASYPDKNSDE
                     MKGLFSDDGVYRTLNASGNKVWLDYITKYFTAN"
     gene            3748..4527
                     /gene="epsA"
     CDS             3748..4527
                     /codon_start=1
                     /gene="epsA"
                     /note="putative chain length regulator"
                     /product="EpsA"
                     /protein_id="AAX19700.1"
                     /transl_table=11
                     /translation="MQETQEQTIDLRGIFKIIRKRLGLILFSALIVTILGSIYTFFIAS
                     PVYTASTQLVVKLPNSDNSAAYAGQVTGNIQMANTINQVIVSPVILDKVQSNLNLSDDS
                     FQKQVTAANQTNSQVITLTVKYSNPYIAQKIADETAKIFSSDAAKLLNVTNVNILSKAK
                     AQTTPISPKPKLYLAISVIAGLVLGLAIALLKELFDNKINKEEDIEALGLTVLGVTTYA
                     QMSDFNKNTNKNGTQSGTKSSPPSDHEVNRSSKRNKR"
     gene            4537..5232
                     /gene="epsB"
     CDS             4537..5232
                     /codon_start=1
                     /gene="epsB"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1216:exopolysaccharide biosynthesis domain protein
                     (Score: 233.2; E-value: 9.2e-71)"
                     /gene_kind="biosynthetic-additional"
                     /note="possible chain length determinant"
                     /product="EpsB"
                     /protein_id="AAX19701.1"
                     /transl_table=11
                     /translation="MAKNKRSIDNNRYIITSVNPQSPISEQYRTIRTTIDFKMADQGIK
                     SFLVTSSEAAAGKSNGSANLAVAFAQQGKKVLLIDGDLRKPTVNITFKVQNRVGLTNIL
                     MHQSSIEDAIQGTRLSENLTIITSGPIPPNPSELLASSAMKNLIDSVSDSFDVVLIDAP
                     PLYAVTDAQILSVYVGGVVLVVRAYETKKESLAKAKKILEQVNANILGVVLHGVDSSES
                     PSYYYYGVE"
     gene            5287..6051
                     /gene="epsC"
     CDS             5287..6051
                     /codon_start=1
                     /gene="epsC"
                     /product="EpsC"
                     /protein_id="AAX19702.1"
                     /transl_table=11
                     /translation="MIDIHCHILPGIDDGAKTSGDTLTMLKSAIDEGITTITATPHHNP
                     QFNNESPLILKKVKEVQNIIDEHQLPIEVLPGQEVRIYGDLLKEFSEGKLLTAAGTSSY
                     ILIEFPSNHVPAYAKELFYNIQLEGLQPILVHPERNSGIIENPDILFDFIEQGVLSQIT
                     ASSVTGHFGKKIQKLSFKMIENHLTHFVASDAHNVTSRAFKMKEAFEMIEESCGSDVSQ
                     IFQNNARSVILNESFYQEKPTKIKTKKFLGLF"
     gene            6070..6759
                     /gene="epsD"
     CDS             6073..6759
                     /codon_start=1
                     /gene="epsD"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1146:sugar transferase (Score: 231.3; E-value:
                     3.8e-70)"
                     /gene_kind="biosynthetic-additional"
                     /note="priming glycosyltransferase; putative"
                     /product="EpsD"
                     /protein_id="AAX19703.1"
                     /transl_table=11
                     /translation="MEVFEDVSSHEPEEHKLVELKKFSHREIIIKRGIDILGGLAGSVL
                     FLIAAALLYVPYKMSSEKDQGPMFYKQKRYGKNGKIFYILKFRTMIINAEHYLELHPEI
                     KAAYHANGNKLENDPRVTKIGSFIRRHSIDELPQFINVLKGDMALVGPRPILLFEAKEY
                     GERLSYLLMCKPGITGYWTTHGRSKVLFPQRADLELYYLQYHSTKNDIKLLSLTIVQSI
                     NGSDAY"
     gene            6763..7212
                     /gene="epsE"
     CDS             6763..7212
                     /codon_start=1
                     /gene="epsE"
                     /note="glycosyltransferase; putative"
                     /product="EpsE"
                     /protein_id="AAX19704.1"
                     /transl_table=11
                     /translation="MKIALVGSSGGHLTHLYLLKKFWENEDRFWVTFDKTDAKSILKGE
                     RFYPCYYPTNRNVKNTIKNTILAFKILRKEKPDLIISSGAAVAVPFFWIGKLFGAKTIY
                     IEIFDRIDKPTLTGKLVYPVTDKFIVQWEELKKVYPKAINLGGIF"
     gene            7212..7697
                     /gene="epsF"
     CDS             7212..7697
                     /codon_start=1
                     /gene="epsF"
                     /note="glycosyltransferase; putative"
                     /product="EpsF"
                     /protein_id="AAX19705.1"
                     /transl_table=11
                     /translation="MIFVTVGTHEQPFNRLIQKIDELVRDGQIKDDVFMQIGYSTYEPK
                     YTKWASVIGYNDMTAYFNKADIVITHGGPSTYMQVLQHGKIPIVVPRQEKFGEHINDHQ
                     LRVSKQVIKKGYPLILCEDVSALKICIESSRIRTDEFIKSNNKNFISNFKKIIASEE"
     gene            7697..8260
                     /gene="epsG"
     CDS             7697..8260
                     /codon_start=1
                     /gene="epsG"
                     /note="galactosyltransferase; putative"
                     /product="EpsG"
                     /protein_id="AAX19706.1"
                     /transl_table=11
                     /translation="MKKFKISVILRTYNEVKHIGEVLKSLTEQTYLNHEIIIVDSGSVD
                     GTLDIIERYPVKLVSINKEDFNYSYASNVGVQNSSGDIVCFLSGHSVPVYKNYLEKINE
                     IFQETEIGACYGEVIALPDGSITEKIFNRIGYLKSKLSLNNKRFFLENKIHPGIFSVVV
                     MLAPERNYYLNTLLKSNSVMEVKM"
     gene            8402..9574
                     /gene="epsH"
     CDS             8402..9574
                     /codon_start=1
                     /gene="epsH"
                     /note="putative polymerase"
                     /product="EpsH"
                     /protein_id="AAX19707.1"
                     /transl_table=11
                     /translation="MLLKKIMTNINRKKFLINFQSLVFFILIIIYGLTTKNVMGGSGIF
                     SIDSILKYGILFICISVEGYIFLKNGNEKRETSENYNNFKFYFIIITFLSLFASFKQVH
                     FSFRTVQSFIFIFIPMLYSYLILNNWTFRQINFSMKIALFLSVIEYLFSIRMGFSQIIS
                     SLASINYNNTNASPLESSTFALLSLGFVAYFGYYKKNFLCKIVSLLFVIMTFKRVITLG
                     GCILVILGILKIKNLRVNRFFLIVSTITLVSFSLIYYYSIQPQNILEISEKIGFSIRDF
                     STNRTDRLAWLSMTDFKSYGLGSTTDFMYKLWGVDLEMDIVQLILEVGAFGVIVFIYFY
                     LRFSKSNLYAFSFMALLLLNSILSSGMMSTFSWIIILIAMSTIMEYKEGM"
     misc_feature    9579..9728
                     /note="similar to glycosyltransferase WciV from
                     Streptococcus pneumoniae and to glycosyltransferase EpsG
                     from Lactococcus lactis B40"
     gene            9749..10591
                     /gene="epsI"
     CDS             9749..10591
                     /codon_start=1
                     /gene="epsI"
                     /note="glycosyltransferase; putative"
                     /product="EpsI"
                     /protein_id="AAX19708.1"
                     /transl_table=11
                     /translation="MKVKIVNTSNKGVSFARNLGINQPSGQYLLFVDSDDELSINALKY
                     LSIMLNKKDRDLILFGFSLTGDNNRKNDTSILKSIANQNTDCKMNILKSILSTKNNILG
                     YVWRAVYSLDFIKKNNIFFETHLKISEDYLFLLQSVEHSNNLFVITEEFYKYNLGETSM
                     SNKFVPTLLNDMVWVNNWIESNILTVYPQFFVGFNCLVANTYIRYEQNAIRNKEENFML
                     KYREIKLNKRKYNFQRSINQVISHLDKFDFKSKIGVILFRIHLDIVYELLFNIKERKN"
     gene            10594..11676
                     /gene="epsJ"
     CDS             10594..11676
                     /codon_start=1
                     /gene="epsJ"
                     /note="glycosyltransferase; putative"
                     /product="EpsJ"
                     /protein_id="AAX19709.1"
                     /transl_table=11
                     /translation="MNIMVDAYLDNNLGDDLMIRYFASYFYQHKIYLVESREHIRKTFY
                     DIPNIYFYSEEDYKMNEYDFQLHVTIGGSMFILDDFKKLIRFRHRIKNSRKIKKRNIPS
                     AIIGCNLGPFDKRNFGLKLAKFELKYKNLVTVRDKQSKELLLRGFKRKKINIKLFPDII
                     FSKVLYKSIPKYGLGISVYRSANSEVNNIQIYQELATLADKFIKRSGEKVALFAFDSEN
                     ENDLVAAHNIKEMIEEKNKVEIIPYLGNSNIFLDKFLSCKKILGIRFHSSILALISGIP
                     LYSLSYSNKTQNMMSNLHLSKYCSPLNNIKENSELIGKDIEDGNLAILKDRDRYELNRL
                     SLEHFIEIEKLINNIYEKGQ"
     gene            11680..12630
                     /gene="epsK"
     CDS             11680..12630
                     /codon_start=1
                     /gene="epsK"
                     /note="putative galactosyltransferase"
                     /product="EpsK"
                     /protein_id="AAX19710.1"
                     /transl_table=11
                     /translation="MDRKSNLPLVSIIITTFNRINYLKQMLESILIQSYKNVEIIIVDD
                     GSVDNTKNIIREYQLKYKQQIIFFEGKKGPGINRFLAYQKISGEFIVFCDDDDYYTDIN
                     FIEKSVNIMYNFQDVSLICFSSDILYVKDNLLETKQLNKKGIFESGEVANNFLTEFQKP
                     NSTFPSFYRRSTFEKNGFTEVKSFNDTNIYLRGILGGKVYYSQKNVGVYRVHSGSIGNS
                     LDPNFIIQVLNEKKILSKYLMTSPKKQEQWLYLQTLNTTDYYADRSSKVEYFTILKWFK
                     LNLSNNYYHRAKKMFIFARIKFLMKKFIKRKNPYI"
     gene            12779..14227
                     /gene="epsM"
     CDS             12779..14227
                     /codon_start=1
                     /gene="epsM"
                     /note="putative repeat unit transporter"
                     /product="EpsM"
                     /protein_id="AAX19711.1"
                     /transl_table=11
                     /translation="MKKVGHNYILNLLYQLLMVILPLITAPYISRTIGAEGIGINAFTA
                     TIAQYFVIVAVLGTATYGNREIAYHQNDKEARSQIFWGITFVSWISATIALVSYIIVIT
                     FLKSYHTIYLLQGLLILTSLFDISWYFMGRENFRVIVFRNFLIKIITLICILIFIKTPL
                     DLTKYVAISTIGGFLGSLSLWPYLRKEIFKPKFKALNLSVHFKYTLALFLPQIMMQVYT
                     ILNRNMIGAFDSIRNLGYYNQADTLIKAVLSVITAFSPVMLPHIAKMHAQNQKKRVKEL
                     IVKSFNIISGLSIALFFGLAGIALNFAPFFWGEDFIKVGILIFVQSPFLIFISWNYIIG
                     GQYLLATNKIKIYSTSIIIAAIVSLITNLILIPTIGVVGASISAILSEFSIFIYQRYNT
                     KEDFSNKELFNQIWKYLLSGLIMFIIIFEINQTFKMNVVQLILQVFLGAIIYIFMNFVL
                     KTKLWEYFYSLLFKIIFKNFKKNL"
     gene            14834..15736
                     /gene="epsL"
     CDS             14834..15736
                     /codon_start=1
                     /gene="epsL"
                     /note="protein potentially involved in polysaccharide
                     biosynthesis"
                     /product="EpsL"
                     /protein_id="AAX19712.1"
                     /transl_table=11
                     /translation="MERKKKKKENIWAIIVPILIIISLIGAWAYALRDSLIPTDYTKTN
                     SSDQPTKTSVSNGYVEQKGEEAAVGSIALVDDAGVPEWVKVPSKVNLDKFTDLSTNNIT
                     IYRINNPEVLKTVTNRTDQRMKMSEVIAKYPNALIMNASAFDMQTGQVAGFQINNGKLI
                     QDWSPGTTTQYAFVINKDGSCKIYDSSTPASTIIKNGGQQAYDFGTAIIRDGKIQPSDG
                     SVDWKIHIFIANDKDNNLYAILSDTNAGYDNIMKSVSNLKLQNMLLLDSGGSSQLSVNG
                     KTIVASQDDRAVPDYIVMK"
     stem_loop       15736..15767
                     /note="putative terminator"
     CDS             complement(15761..16663)
                     /codon_start=1
                     /note="OrfY"
                     /product="unknown"
                     /protein_id="AAX19713.1"
                     /transl_table=11
                     /translation="MNQKKRRHYRKKKHTVLKVISIIFVLVIIAVASIAYAAYRNVEST
                     FSTSYENFPKTTSIDLKKSKTFTTLIIATGKNNSKNTAYATVLASTNVKTNQTTFMNFP
                     VFATMPNQKTITEVYNTNGDDGIFQMVKDLLNVSINKVIQIDVNKMGSLVQASGGITMQ
                     NPKAFNAEGYEFKQGTVNLQTADQVQAYMTQIDDTDLDASITRIQNVSMELYGNIQKIA
                     HMKKLESFNYYREILYAFSNTVKTNISFNDAKTIVMSYNKALKNTSKLNLHTTDENGAK
                     VVSQTELDSVKILFEKSLK"
     misc_feature    16796..16909
                     /note="similar to transposase orf1 of IS981 from
                     Lactococcus lactis IL1403"
ORIGIN
        1 cctttcaaaa gcaaattcgt aaagtgatag aagagaaaat aaaaaacgga aacgaaattt
       61 ttcaaccaat tggtaaaaag aagtcaccaa aaaaagctaa aaatcctgca agagcctatc
      121 gtcaagaggt taaggactct attattgacg aagagctgtt tgactacttg cgggaacaat
      181 ctaagaaaga tacaattgaa cgttttaaga gccgctcaga ttacgatagc gaagctttgg
      241 ccatgaacgc tgactttcag gagtttgttg aaaaacagac gttcccagaa gaagtaggac
      301 gctgggaggg tttgactgaa gctcatgata gagtaaaaaa aaaagagcaa atatcatttc
      361 agaagcactg tttgcttact tgtcttctaa tcgattttct tattcaacaa aagaaatttc
      421 atctttctta cccatttttg accctgtgtt cataagggaa aaactatttt ttgaaaattt
      481 tgaattagat ctaaaagggt tagtgagtga catgaatcat tatgatagct tattcgaatg
      541 ccaaggagat ttatcgggga aggatcggca acttgaggat aaacttaagg attggaaaag
      601 ttatgcttgg ttaaaaaata aatgaggatt aagatggact aactttcagt caattaggat
      661 aaaaaagaga aaacaaaaaa gaagttcaca tcaaaatgat gtgaacccca aaagctagac
      721 tttttagaca agaataattt cttgtctttt ttttgttatt ttgaataagt taaacgattt
      781 ggattgactc atccagttca attttttctt aattcgtttc gtgttgtaat agtgaatcca
      841 tttagtaatc gcttgttcaa gttcttcaaa tgaattgaaa acatgaccat agtagatttc
      901 ttgtttaagc aagccaaaga agttctccat gactgaatta tcatgacagt tacctttgcg
      961 actcatagat tggattattt tttgtgattt gagcttaaag acatagtttt tcatctgata
     1021 tgcccaccct tggtcagagt ggaaagtacg acgatagaga caatcagaag tcaattctaa
     1081 agcctcctga agtgctgtcg caatagcttg ataggttggt tggttagaga tttcatagcc
     1141 aatcacttcg ttgttgaaaa ggtcaagata ggggttgaga taaagcttct tttgaacccc
     1201 tttatcgtaa tatttgaact cagtggtgtc tgtcgtatct tttgatgtgg tacagaagtg
     1261 ttaaatcgtc tgtggagctt gttcttagcc acagtcccaa ctttaccttt gtaagagtta
     1321 tatttacgag atttatgcca aaaagtagtc acacgaagcc caagtttttt catcaagccg
     1381 ctgaaccttc ttgtgattaa cataaattcc acgttgttta atagctcaac cattggacga
     1441 taccctgcat tagtgtgtnc ttgacgtatt tcaagcatga cttgctcaat catttcatct
     1501 ttattaggac gttcaaacga ttgacccagt agtaataagt gctttagaaa gttcagaaat
     1561 ggctaagagt tgcttaagtg ggaacttgaa ttctcgtcgg agttcacaga ccactcggga
     1621 ttttccctct tgtgttgttc ctgccgtaat ctcctcagcc cttttaaata ttcaacttcc
     1681 attcttaaat taagattgtc gttttgaagc ctagcaagtt ctgctctgtt tcattcacca
     1741 catctttcgc tttcttaata cttatttcaa gcaattcagg ctcttttcta ggtctcccct
     1801 gaacattaga aaaggcaaat tccccttttt tgcgataatt tgacacccat cgtgtaagaa
     1861 gtggcggatt attcattccc atttcatgaa ctagttctcc atatgacttt tcacttgtta
     1921 gatataagtt taccgcatta agtttgaatt gtgttgaata aactgtattt tgtcttttac
     1981 gctgaattcc aaccgcccct aattctttat aagctttgac ccaacgcctg acaatcgctt
     2041 cgttcgtgac atggtacttt ttggctgaat aatcacaccc ccctacaccg ttatcataat
     2101 cctgaaccac ttttagtttt agttcaaatg aatattttgt cataataaaa gacctttcat
     2161 ctatcttttg ggggtcacta cagatttggt aaacttcttt tttatcattt attttttatt
     2221 aaattgctct aatacttttt taatccataa tcttaatatg taggattttt tattcttgta
     2281 tttaatagaa ggtttaagat agagttaagg cgataacttt caggaaaatt ttaatttttc
     2341 tgaaagtaaa gttgaaaaac tatactaaat gattaaactt atatctttgc ttatacgtaa
     2401 aatttcttgc agtttgaagt ttgaaaccgt attgtcactt gcattaatag gtttcgtttt
     2461 atataattaa tacaatattt aaaattaaaa aaagcaccaa aatggtgtta acttaagtaa
     2521 gttttgattt aatttttctg aaaaattaaa atttttctta cagaagttaa taaaaagagg
     2581 attatgttta tgaatgattt attttaccat cgtctaaagg aactagttga agcaagtggt
     2641 aaatctgcaa atcaaataga aagggaattg ggttacccta gaaattcttt gaataattat
     2701 aagttgggag gagaaccctc tgggacaaga ttaataggac tatcagagta ttttaatgtg
     2761 tctccaaaat atctgatggg tataattgat gagcctaatg acagttctgc aattaatctt
     2821 tttaaaactc taactcaaga agagaaaaaa gaaatgttta taatttgtca aaaatggctt
     2881 tttttagaat atcaaataga gttataacaa taataaattt agggagtttt tcttattgat
     2941 atgatgaaaa aaggaatttt tgtaattact atagtgatat ctatagcatt tataattgga
     3001 ggtttttata gttataattc taggataagt aatctttcaa aagctgataa aggaaaagaa
     3061 gttgtaaaaa atagcagtga aaaaaatcag atagacctta cctataaaaa gtattataaa
     3121 aatttaccaa aatcagttca aaataaaata gatgatattt catccaaaaa taaagaagtt
     3181 actttaactt gtatttggca atctgattca gttatttctg aacaatttca acaaaactta
     3241 caaaaatatt atggaaataa gttttggaac atcaaaaata tcacttacaa tggcgaaact
     3301 agtgaacaat tattggctga aaaagttgaa aaccaagtat tagccactaa tcctgatgtt
     3361 gttttatatg aagctccact ttttaatgat aaccaaaaca ttgaagcaac agcctcactg
     3421 actagtaatg agcaacttat aacaaatttg gctagtgcag gagcggaggt aatagttcaa
     3481 ccctctccac cgatctatgg tggtgttgtg taccccgtac aagaagaaca atttaaacaa
     3541 tctttatcta caaagtatcc ctatatagac tactgggcta gttacccaga caaaaattct
     3601 gatgaaatga agggactgtt ttctgatgat ggagtatata gaacattaaa tgcttcgggg
     3661 aataaggttt ggctagatta tattactaaa tattttacag caaactaatt aagttataaa
     3721 taacaattat taaatattgg agaagaaatg caggaaacac aggaacaaac gattgattta
     3781 agagggattt ttaaaattat tcgcaaaagg ttaggtttaa tattatttag tgctttaata
     3841 gtcacaatat tagggagcat ctacacattt tttatagcct ccccagttta cacagcctca
     3901 actcaacttg tcgttaaact accaaattcg gataattcag cagcctacgc tggacaagtg
     3961 accgggaata ttcaaatggc gaacacaatt aaccaagtta ttgttagtcc agtcatttta
     4021 gataaagttc aaagtaattt aaatctatct gatgattctt tccaaaaaca agttacagca
     4081 gcaaatcaaa caaattcaca agttattacg cttactgtta aatattctaa tccttacatt
     4141 gcacaaaaga ttgcagacga gactgctaaa atatttagtt cagacgcagc gaaactattg
     4201 aatgttacta acgttaatat tctatccaaa gcaaaagctc aaacaacacc cattagtcct
     4261 aaacctaaat tgtatttagc aatatctgtt atagccggat tagttttagg tttagccatt
     4321 gctttattga aggaattgtt tgataacaaa attaataaag aagaagatat tgaagctctg
     4381 ggactcacgg ttcttggtgt aacaacctat gctcaaatga gtgattttaa taagaatacg
     4441 aataaaaatg gcacgcaatc gggaactaag tcaagtccgc ctagcgacca tgaagtaaat
     4501 agatcatcaa aaaggaataa aagataggag ttcaggatgg ctaaaaataa aagaagcata
     4561 gacaacaatc gttatattat taccagtgtc aatcctcaat cgcctatttc cgaacaatat
     4621 cgtacgattc gtacgaccat tgattttaaa atggcggatc aagggattaa aagttttcta
     4681 gtaacatctt cagaagcagc tgcaggtaaa tcaaacggga gtgctaatct agctgttgct
     4741 tttgcacaac aaggtaaaaa agtactttta attgatggcg atcttcgtaa accgactgtt
     4801 aacattactt ttaaagtaca aaatagagta ggattaacca atattttaat gcatcaatct
     4861 tcgattgaag atgccataca agggacaaga ctttctgaaa atcttacaat aattacctct
     4921 ggtccaattc cacctaatcc atcggaatta ttagcatcta gtgcaatgaa gaatttgatt
     4981 gactctgtgt ccgattcctt tgatgttgtt ttgattgatg ctccacctct ctatgcagtt
     5041 actgatgctc aaattttgag tgtttatgta ggaggagtgg ttcttgttgt acgtgcctat
     5101 gaaacaaaaa aagagagttt agcaaaagca aaaaaaatac tggaacaagt taatgcaaat
     5161 atattaggag ttgttttgca tggggtagac tcttctgagt caccgtcgta ttactactac
     5221 ggagtagagt aattggaata aattttaatc aaataaaagt cagaaatttg tagaagaggg
     5281 gagcaaatga ttgatattca ttgccatatt ttaccgggga tagatgatgg agctaaaact
     5341 tctggagata ctctgacaat gctgaaatca gcaattgatg aagggataac aactatcact
     5401 gcaactcctc atcataatcc tcaatttaat aatgaatcac cgcttatttt gaaaaaagtt
     5461 aaggaagttc aaaatatcat tgacgaacat caattaccaa ttgaagtttt acccggacaa
     5521 gaggtgagaa tatatggtga tttattaaaa gaattttctg aagggaagtt actgacagca
     5581 gcgggcactt caagttatat attgattgaa tttccatcaa atcatgtgcc agcttatgct
     5641 aaagaacttt tttataatat tcaattggag ggacttcaac ctattttggt ccaccctgag
     5701 cgtaatagtg gaatcattga gaacccggat atattatttg attttattga acaaggagta
     5761 ctaagtcaga taacagcttc gagtgtcact ggtcattttg gtaaaaaaat acaaaagctg
     5821 tcatttaaaa tgatagaaaa ccatctgacg cattttgttg catcagatgc gcataatgtg
     5881 acgtcacgtg catttaagat gaaggaggca tttgaaatga ttgaagaaag ttgtggttct
     5941 gatgtatcac aaatatttca aaataacgca aggtcagtga ttttaaacga aagtttttat
     6001 caagaaaaac caacaaagat caaaacaaaa aaatttttag gattatttta aagggattaa
     6061 atggagtaaa taatggaagt ttttgaggat gtctcatcac atgaaccgga agagcataag
     6121 ttagtagaat taaaaaaatt ttctcataga gagataatta taaaaagagg gattgatatt
     6181 ttagggggat tagcgggttc agttttattt cttattgcgg ctgcattgct ttatgtccct
     6241 tacaaaatga gctcggaaaa agatcaaggg ccaatgttct ataaacaaaa acggtatgga
     6301 aaaaacggta aaatttttta tattttgaaa tttagaacaa tgataattaa tgctgagcat
     6361 tatctagagc tccatccaga aattaaagct gcctaccatg ccaatggcaa caaattagaa
     6421 aatgatccac gggtaacgaa gattgggtca tttatcagac gacactcaat tgatgagtta
     6481 ccacaattta tcaatgttct taaaggggat atggcattgg ttggcccaag accaattttg
     6541 ctttttgaag cgaaagaata tggggagcgc ctctcttact tactcatgtg taaacctgga
     6601 attactggtt attggacaac acatggtcga agtaaagttc tttttcctca acgagcagat
     6661 ttagaactct attacctcca gtaccatagc accaaaaacg atatcaagct tctatcactc
     6721 acaattgtac aaagtattaa cggatcggac gcatattaaa aaatgaaaat agcattagta
     6781 ggttccagcg gtggccattt gacacacctg tatttgttaa aaaagttttg ggaaaacgaa
     6841 gatagatttt gggtcacatt tgataaaaca gatgcaaaat ctatattgaa aggagaaaga
     6901 ttttatcctt gttattatcc cacaaataga aatgtaaaaa acacgataaa aaatactatt
     6961 cttgcattta aaatacttag aaaagaaaaa ccagatttga ttatttcgag tggtgctgcg
     7021 gtagccgttc cttttttctg gataggtaaa ctattcggtg caaaaacaat ctatattgaa
     7081 atatttgacc ggattgataa accaacctta acaggaaaat tagtttatcc agttactgat
     7141 aagtttatag ttcaatggga agagttaaaa aaagtctatc ctaaagcgat taatctagga
     7201 gggattttct aatgattttt gtaactgttg gaactcacga acaaccattt aatcgactca
     7261 ttcaaaaaat tgatgaactt gtacgtgacg gtcaaataaa agatgatgta ttcatgcaaa
     7321 ttggttactc gacttatgag ccaaaatata caaaatgggc gtcagtgata gggtataatg
     7381 atatgactgc atattttaat aaagcagaca tcgtgattac acacggaggt ccttcaacct
     7441 atatgcaagt tttacaacat ggtaagattc caattgtagt accacggcaa gaaaagtttg
     7501 gtgaacatat taacgatcac caattacggg taagtaaaca agtgattaaa aagggatatc
     7561 ctcttatatt gtgcgaagat gtctcagctt tgaagatttg tattgaaagt tcaaggatta
     7621 gaacagatga atttattaaa agcaacaata agaattttat atcaaatttt aaaaaaataa
     7681 tagcttccga ggaatgatga aaaaatttaa aattagtgta atattaagaa cttataatga
     7741 agttaaacat attggtgaag ttcttaaaag tttaacagaa cagacatatt taaaccatga
     7801 aattattata gttgattctg gttcagtgga tggaacctta gatataatag agcggtatcc
     7861 agtaaaactt gtatcaatta ataaagaaga ctttaattat agttatgctt caaatgttgg
     7921 tgttcaaaac tcctcagggg atattgtatg ttttttgagt ggacattcgg tgccagttta
     7981 taaaaattac cttgaaaaaa taaatgagat ttttcaagaa actgagatag gtgcttgtta
     8041 tggagaagtg attgccttac cagatggaag cattacggaa aagatattta atagaatagg
     8101 ttatctaaaa agtaagttaa gtctcaataa taaaagattt tttttagaaa ataaaattca
     8161 tccaggtata ttttcagttg tagtaatgct tgcgccagaa agaaattatt acttaaatac
     8221 ccttttaaag tcgaactcgg tcatggaggt gaagatgtag aagtggctta tagaattatt
     8281 caagatggat attttgtagc aaaatcagtt gaattgctcg taatgcattc tcacggaagt
     8341 tcattaaaaa aatttataaa ggaatataaa gcttggggta aaatgtatga agatgtccta
     8401 aattttatta aaaaaaataa tgacaaatat caatagaaaa aaatttttga taaattttca
     8461 aagtttggtt tttttcatat taataattat ttacgggcta actacaaaaa atgtcatggg
     8521 aggatcaggt atcttttcaa ttgatagcat attaaaatat ggtatcttat ttatttgtat
     8581 tagtgtagaa gggtatatat tcctaaaaaa cggaaatgaa aaaagggaaa cttctgaaaa
     8641 ttacaataat tttaagtttt attttattat aatcactttt ttatctcttt ttgcttcttt
     8701 taaacaagta cattttagtt tcagaacggt ccaatctttt atatttattt ttattcctat
     8761 gttatattca tatctaatct taaataattg gacttttaga cagatcaatt tttcaatgaa
     8821 gatagcatta ttcttatcag ttatagagta tttattctca attaggatgg gtttttctca
     8881 gattatcagc agtctagctt caataaatta caataataca aatgcttcac cattagaatc
     8941 ctcaaccttt gcactcctat cattaggatt tgttgcttat tttggatatt ataagaaaaa
     9001 ttttttatgt aaaatagtta gtttgctatt tgttattatg acatttaaaa gagttattac
     9061 tctaggtggt tgtattttgg ttattttagg tatacttaaa attaaaaatc ttagggttaa
     9121 taggtttttt ttgatagttt caacaattac attggtgtca ttttcattaa tttattatta
     9181 ttcaattcaa ccccaaaata tattagaaat cagtgaaaaa ataggcttca gtattagaga
     9241 tttttcaaca aatcgtacag ataggttagc ttggttaagt atgactgatt ttaaaagtta
     9301 tggtcttggc tccactactg actttatgta taagttatgg ggtgttgatt tagaaatgga
     9361 tattgtacaa cttattttag aagttggtgc ctttggagta attgttttta tatattttta
     9421 tcttagattt tcaaaaagca acctatatgc gttctcattt atggccttat tgttactaaa
     9481 ctcaatattg tcatcgggta tgatgtcaac tttttcttgg ataataattt taattgcaat
     9541 gagtaccatt atggaatata aagaaggaat gtagtataat gaaaaataaa tttagtataa
     9601 ttgttcctgt atataatggg gaaagtcata taaaaaaatg tatagatact ctattaaagc
     9661 agacttataa tgattttgaa attattatta ttaatgatgg gtctacgatg acactaagtc
     9721 agttttaaca aagttttacg ccaaaaattt gaaagtaaaa atagtaaata cttcaaataa
     9781 aggtgtcagc tttgccagaa atttaggaat taatcaacca tcaggacagt atttgctatt
     9841 tgttgattct gatgacgaat tatcaataaa tgcactcaag tatttatcaa taatgttgaa
     9901 caaaaaagat agagatttaa ttttatttgg tttttcacta acaggtgaca ataatagaaa
     9961 aaatgataca agcattttaa agtctatagc aaatcaaaat acagactgta agatgaatat
    10021 tctaaaatca attctgtcaa caaaaaataa tattctaggg tatgtgtgga gagcagttta
    10081 ttctttagac tttattaaga aaaataatat attttttgag acacatttaa aaatatcaga
    10141 agattatcta tttttgctac aaagtgttga acactcaaat aatttatttg ttataacaga
    10201 agaattttac aaatataatt tgggagagac ctctatgtca aataaatttg ttccaacttt
    10261 attaaatgat atggtctggg taaataattg gattgaatca aacatactaa ctgtatatcc
    10321 tcagtttttt gttggtttca attgtcttgt tgctaatacc tatattcgtt atgaacaaaa
    10381 cgcaattcgt aacaaagaag aaaattttat gttgaaatac cgagaaataa aacttaataa
    10441 aaggaaatat aattttcaga gatcaataaa tcaagttatt tctcatttgg ataagtttga
    10501 ttttaaatca aaaattggag ttattttatt taggatccat cttgatatcg tttatgaatt
    10561 gttatttaat attaaagaaa gaaaaaatta aatatgaata taatggtgga tgcctactta
    10621 gataataatt taggtgacga tttgatgatt agatattttg cgtcttattt ttatcaacat
    10681 aaaatatatt tagttgaatc tagggaacat atccggaaaa cattttatga tatacccaat
    10741 atttattttt atagcgagga agattataaa atgaatgaat atgattttca gctacacgtg
    10801 acaataggtg gttctatgtt tattttagac gattttaaaa agctaattag atttagacat
    10861 cgaataaaaa attctagaaa aataaaaaaa agaaatattc cctcagctat tataggatgc
    10921 aatttgggtc catttgataa aagaaatttt ggattgaaac ttgcaaaatt tgaattaaag
    10981 tataaaaatt tggtaaccgt tagagataaa caatccaaag aattgttgct tagaggattt
    11041 aaaagaaaaa aaattaatat aaaacttttt cctgatataa tatttagtaa agtcttgtat
    11101 aaaagtattc cgaagtatgg gttaggtatt tcagtatatc gttcagctaa ttcagaagta
    11161 aataatattc aaatttatca agaattagca acgcttgctg ataagtttat aaaaagaagt
    11221 ggagaaaaag ttgctctttt tgcttttgat tcagaaaatg aaaatgactt agtagctgca
    11281 cacaatataa aagagatgat agaagaaaaa aataaagttg aaattattcc gtatttaggg
    11341 aattcaaata tttttttgga taaattttta agctgtaaga aaatactggg tattagattt
    11401 catagttcca tactagcttt gatatctggt attcctttat attcactatc atattctaat
    11461 aaaactcaga atatgatgtc aaatctccac ctatcaaagt attgttcacc actaaataat
    11521 attaaagaaa atagtgaatt aataggcaaa gatatcgaag atgggaatct tgctatttta
    11581 aaagatagag atagatatga gttaaataga ttaagtttag aacattttat tgaaatcgag
    11641 aaattaatta ataatattta tgaaaagggt cagtaaaaaa tggatcgcaa atcaaatctc
    11701 ccacttgtat cgattataat aacaacattt aataggatta attatcttaa acaaatgctt
    11761 gagagcatac taattcaatc ttataaaaat gtagaaatta ttattgttga tgatggatca
    11821 gtagataata ctaaaaatat tataagagaa tatcaactaa agtataaaca acaaataatt
    11881 ttttttgaag gtaagaaagg gccaggaata aatagatttt tagcatatca aaagatttca
    11941 ggagagttta ttgtattttg tgacgatgat gattactata ccgacattaa ttttattgaa
    12001 aaaagtgtaa atatcatgta taacttccaa gatgtttctc taatttgctt cagttccgac
    12061 attttatatg ttaaagataa tttacttgaa acaaaacaat taaataaaaa aggtattttt
    12121 gagagcggag aagttgcaaa caatttttta actgagtttc aaaaacctaa tagtactttt
    12181 ccttcctttt acagaagaag tacatttgag aaaaatggtt ttactgaagt taaaagcttt
    12241 aatgatacaa acatttatct aagaggaatt ttaggtggga aggtatatta ttcccaaaaa
    12301 aatgttggtg tttatcgagt tcattcaggg tctataggta attcattgga tcctaatttt
    12361 attattcaag tactgaatga aaaaaaaatt ttaagcaagt atttaatgac ttcccccaaa
    12421 aagcaagaac aatggcttta tcttcaaaca cttaatacaa cagattacta tgctgataga
    12481 agtagcaaag tggaatactt taccatacta aaatggttta aattaaattt aagcaacaac
    12541 tattaccaca gagcaaaaaa gatgttcatt tttgcaagaa tcaaatttct aatgaagaaa
    12601 tttataaaga gaaaaaatcc atatatatag gtatatttca cgtaaaaatt agtataaaat
    12661 taaaaaatta tatttaagaa aaccaaaggc ataaagtatt gtaatataca tcttggtata
    12721 tgtatgcttt ttttaattaa aatcttcggt tattttattt tattttatag gagtagggat
    12781 gaaaaaagtt ggccataatt atatcctaaa tttactttat cagttattaa tggttattct
    12841 cccgttaata actgcaccat atatttctag aactatagga gcggaaggga tcgggataaa
    12901 tgcatttaca gctacaattg cacagtattt tgttattgta gctgttcttg gcactgcgac
    12961 ttatggaaat agagaaattg catatcatca gaatgataag gaagcgagaa gtcaaatttt
    13021 ttggggtatt acatttgtta gttggatatc tgctacgatt gctcttgtgt cttatataat
    13081 agttattact tttttgaaga gttaccatac aatatattta ttgcagggtt tattaatttt
    13141 gacctcttta tttgatattt cttggtattt tatgggaaga gaaaactttc gagttatagt
    13201 gtttagaaat ttcttgataa aaattatcac tctcatttgt atacttattt ttattaaaac
    13261 tcctttagat ttaactaaat atgtagctat aagtacaatt ggcgggtttt tgggtagttt
    13321 atcattatgg ccctatttaa gaaaagaaat atttaaacca aaattcaagg cacttaattt
    13381 aagtgtacat tttaaatata cactagcact attcttacca caaattatga tgcaagtata
    13441 tacaattcta aatagaaata tgatcggtgc ctttgactct attagaaact taggatacta
    13501 caaccaagca gatactttga ttaaagcagt attatcagtt ataacagctt ttagtccagt
    13561 aatgcttcca catattgcaa aaatgcatgc tcaaaatcaa aaaaaaagag tcaaggaact
    13621 tatagtaaaa tcatttaata tcatttcagg cttaagtatt gcactatttt ttggattagc
    13681 agggattgca ttaaactttg ctcctttttt ttggggggaa gacttcatca aggtaggtat
    13741 attaatattt gtacaatcac cctttctaat attcattagt tggaattata taataggcgg
    13801 ccaataccta ttggcgacaa ataaaatcaa aatttattca acatcaatta ttattgcagc
    13861 aatagtgagc ttaattacaa atttgatact aattcctact attggagtgg ttggtgcatc
    13921 aatatcggca attctttcgg agttttcaat atttatatat cagagatata acacaaaaga
    13981 agatttttca aataaagaat tatttaatca aatatggaag tatttactat ctggattaat
    14041 tatgttcatt attatatttg agataaatca aacatttaaa atgaatgttg tacaactaat
    14101 attgcaagta tttttaggag caataatata tatttttatg aattttgttt tgaaaacaaa
    14161 attatgggaa tatttttata gtttactttt taaaattata tttaaaaatt ttaaaaagaa
    14221 tttataattg tggttgtcat tgattttttt cttaaaaata tgttccaaaa atgaaataac
    14281 tagtatcatt ttttgtttta tttagttaaa agagaaccgc actacaataa ttcacaattt
    14341 ataattatat ttgagaaacc atttacgaaa atgatttcaa actcttttat aaattgtacc
    14401 gtagcataag ctactaagct catgcaaaat atatatttag cttattagtt tataatattt
    14461 tgatatagat taataattat taatgtgcta taatacttta ttagctgatc tagtgtttat
    14521 taaaagattc ataattatat tgggaaaaaa tattagtata aaattatcag atatgctgct
    14581 tgacgatgaa atgaattatg aaaatttcaa actaattaat ttaaaacact ggttgaacta
    14641 attgggagca tagttatcca tacaaatatt ataatttcta taattctcgt gtaaatagta
    14701 atctggataa gtttaaaaat atttcttatg taattgtttc aaatcattcc actaaagatc
    14761 ttaaagattt taaaactaaa gcatacagga gagatttatt tggatgtgac caaacaacaa
    14821 ttttggagga taaatggaac gaaaaaagaa gaagaaagag aatatttggg caataattgt
    14881 acctatctta ataataattt cccttatagg agcatgggct tatgccttaa gagattcact
    14941 tattcctact gattatacaa aaacaaacag ctcggatcaa ccgaccaaaa cttcggtttc
    15001 taacggttat gtagaacaaa aaggtgaaga agctgctgtg ggtagtatag cacttgtaga
    15061 tgatgctggt gtaccagaat gggttaaagt tccctcaaag gtaaatctag ataaatttac
    15121 tgatttatct acgaataata tcactattta tcgaattaac aatccggaag tcttaaaaac
    15181 agttaccaat cgtacggatc aacggatgaa aatgtcagaa gttatagcta agtatcctaa
    15241 tgctttgatt atgaatgctt ccgcatttga tatgcagaca ggacaagtag ctggatttca
    15301 aattaataat ggaaagttga ttcaagactg gagtccaggt acaacgactc aatatgcttt
    15361 tgttattaac aaagatggtt cgtgcaaaat ttatgattca agtacacctg cttcaactat
    15421 tattaaaaac ggagggcaac aagcctatga ttttggtact gcaattatcc gtgatggtaa
    15481 aattcaacca agtgatggct cagtagattg gaagatccat atttttattg cgaatgataa
    15541 agataataat ctctatgcta ttttgagtga tacaaatgca ggttatgata atataatgaa
    15601 atcagtgtca aatttgaagc tccaaaatat gttattactt gatagtggtg gttcaagtca
    15661 actatctgtc aatggtaaaa cgattgttgc tagtcaagat gatcgagccg taccggatta
    15721 tattgtgatg aaataaaaat aaaagaacct cttggttctt ttattttaga gatttttcaa
    15781 aaaggatttt gactgagtct aattctgttt gagaaactac cttagctcca ttttcatctg
    15841 ttgtatgtag attgagcttg ctggtattct ttagagcctt attgtagctc ataacgatcg
    15901 ttttagcatc attgaaactt atattggttt taacagtgtt tgaaaaagca tagagaattt
    15961 ctcgatagta attgaaactt tcaagttttt tcatatgagc aattttttga atatttccgt
    16021 agagttccat tgagacattt tgaatccgag tgattgaagc atccaaatca gtatcatcaa
    16081 tttgtgtcat ataggcttgg acttgatcag cagtttgtaa attaacagtt ccttgtttaa
    16141 actcataacc ttcagcattg aatgcctttg gattttgcat ggtgattcca ccagaggcct
    16201 gtacaagtga tcccatttta ttaacatcga tctgaattac tttgttaatg gacacattca
    16261 ataggtcttt aaccatctgg aaaattccat catctccatt cgtattgtaa acttcagtga
    16321 ttgttttttg attaggcatt gtcgcaaaaa ctgggaagtt catgaaagta gtttgatttg
    16381 tctttacatt cgttgaagct aaaacagtag cataagctgt atttttagaa ttatttttac
    16441 cagttgcaat gataagtgtg gtgaatgttt tagacttttt taagtcaata cttgttgttt
    16501 tagggaaatt ttcatatgat gttgaaaatg ttgattcaac atttctataa gcggcgtagg
    16561 ctatagaagc aacagcgata attactaata caaaaataat tgaaataact tttagtactg
    16621 tgtgtttttt cttacgataa tgacgcctct ttttttgatt catggtatct ccatatatat
    16681 attatatacc ttaaattata ccatatttaa taacaaattt aaatgtaaga atagtgatgt
    16741 tatcttaaag cttagaggtg tattgtataa tttagacaaa ggacaaaatc atgcaaaaga
    16801 atttaaagaa acctttatcg acttctacca ttctgggcaa ttcatcactc agctttctaa
    16861 aaaaaacaac gtgactcatt caacgattta taaatcgatt gacctctaat gtccaatgaa
    16921 gaattactct caaaggctga tgctctagaa tttaaaatat gat
//
