LOCUS       BGC0000772             13835 bp    DNA     linear   BCT 05-JUN-2000
DEFINITION  Brucella melitensis strain 16M lipopolysaccharide O side chain
            biosynthesis gene cluster, complete sequence.
ACCESSION   BGC0000772
VERSION     BGC0000772
KEYWORDS    .
SOURCE      Brucella melitensis
  ORGANISM  Brucella melitensis
            Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
            Brucellaceae; Brucella/Ochrobactrum group; Brucella.
COMMENT     On Dec 30, 1998 this sequence version replaced gi:3170022.
            ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-07 20:32:11
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 13835
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..13835
                     /db_xref="taxon:29459"
                     /mol_type="genomic DNA"
                     /organism="Brucella melitensis"
                     /strain="16M"
                     /type_material="type strain of Brucella melitensis"
     subregion       1..13835
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000772"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..13835
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            complement(961..2091)
                     /gene="wbkA"
     CDS             complement(961..2091)
                     /codon_start=1
                     /gene="wbkA"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1045:glycosyl transferase group 1 (Score: 192.2;
                     E-value: 2.7e-58)"
                     /gene_kind="biosynthetic-additional"
                     /note="WbkA"
                     /product="mannosyltransferase"
                     /protein_id="AAC98618.1"
                     /transl_table=11
                     /translation="MCKVMRIGVDARNLVNNITGISRYVMENCRELYERGHELVLYTPE
                     KPRVQLSSSEGFDFRVSNFQGPAGRLFWSQTVLPLQLRKDRIDVFWGPAHRLPALKGAI
                     PSVVSIHDLVWYYASSTMRLQGWLADRFLMKGALRNADQIVAVSYATSSAISSVFPQYT
                     SKIQMVYPGVSHFNRIGTSNILKEHRINKSYGLFIGTLEPRKNLIRLLEAYANLPECVR
                     NNFLLVVAGGKGWNLGDIGKTINRLGIAEYTRLTGYVTDSDLNDLYRNARTLLMPSLYE
                     GFGLPIIEAQAYGVPVITSNCSSMPEVAGDAAILVDPLNVAEISNALYRIATDNKLWQR
                     KSLLALQNIERFNWHNSIEKLESVFIKARESKLMTY"
     repeat_region   complement(2197..4850)
                     /note="disrupted"
                     /rpt_family="ISBm1"
     CDS             complement(join(2210..2416,4268..4534))
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1097:transposase
                     (Score: 117.7; E-value: 9.4e-36)"
                     /gene_kind="other"
                     /note="disrupted by IS711 (IS6501)"
                     /product="P2 ISBm1"
                     /protein_id="AAC98623.1"
                     /transl_table=11
                     /translation="MCPRSPARGHRKKGDGDDGGMGRSRGGLTSKIHALVDAEGRPVNL
                     RLTGGQIADCAEADALTNEFGEGDILLADKGYDTNAIRARCLVPAWANIPPKSNRKDSF
                     VFSRWVCRQRSLVERFFNRIKQFRDIATRYDKRPENYLAAVKLVATRIWCQSL"
     misc_feature    complement(2210..4820)
                     /note="contains putative ISBm1 transposase coding region;
                     -1 frameshifting may result in the fusion of two
                     overlapping ORFs (P1 and P2 ISBm1)"
     repeat_region   complement(2436..3277)
                     /rpt_family="IS711"
     CDS             complement(join(4393..4652,4652..4820))
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1097:transposase
                     (Score: 111.2; E-value: 9.1e-34)"
                     /gene_kind="other"
                     /product="P1 ISBm1"
                     /protein_id="AAC98620.1"
                     /transl_table=11
                     /translation="MTRRRYELTDHEWSIISPLLPNKPRGVARVDDRRVLNGILWRFRT
                     GSPWAEVPERYGPAAACYNRFVRWRKAGVWDRLFETVPKAYDGDIVMIDSTCVRVHQHA
                     ATGKKGMETMVAWDVPAAGLRAKSTRSLMPKVARSIYV"
     gene            5338..6426
                     /gene="gmd"
     CDS             5338..6426
                     /codon_start=1
                     /function="catalyzes the conversion of GDP-mannose to
                     GDP-4-keto-6-deoxymannose"
                     /gene="gmd"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010:NAD-dependent epimerase/dehydratase (Score:
                     283.1; E-value: 5e-86)"
                     /gene_kind="biosynthetic-additional"
                     /note="Gmd"
                     /product="GDP-mannose dehydratase"
                     /protein_id="AAC98612.1"
                     /transl_table=11
                     /translation="MKKVALITGITGQDGAYLAELLLQKGYAVHGIKRRTSQFNTARID
                     HLYHDPHEQGVDLTLHHGDLTDTSSLVRIMQLVRPDEVYNLGAQSHVAVSFEEPEYTAN
                     SDALGALRLLEAIRILGMEKQTRYYQASTSELYGLVQEIPQKETTPFYPRSPYAAAKLY
                     AYWITVNYREAYGIYACNGILFNHESPLRGETFVTRKITRALARIKIGNQNRLYLGNLD
                     SLRDWGHARDYVEMQWLMLQQDQPEDFVIATGKQYSVREFVTLAAKDIGIEMRWEGSGE
                     AEKGYDAKTGACIVEVDPRYFRPAEVETLLGDATKAHQKLGWKPKISFETLVSEMIAED
                     LAAAARENLLREHGHQFFAPKE"
     gene            6434..7537
                     /gene="perA"
     CDS             6434..7537
                     /codon_start=1
                     /function="catalyzes the conversion of
                     GDP-4-keto-6-deoxymannose to 4-NH2-4,6-dideoxymannose
                     (perosamine)"
                     /gene="perA"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1056:DegT/DnrJ/EryC1/StrS aminotransferase (Score:
                     347.2; E-value: 1.9e-105)"
                     /gene_kind="biosynthetic-additional"
                     /note="Per"
                     /product="perosamine synthetase"
                     /protein_id="AAC98613.1"
                     /transl_table=11
                     /translation="MDIPVYSPYLCGNFKKYVNECLDTGWISSRGEFISRFEDAFAQYV
                     DVPSAASVANGTVALHLALDALGIGAGDEVIVPTFTYIASVNTILQTGATPVYVDSLEN
                     TLQIDPEGVRLAITERTKAVMVVHLYGHPCDMDSIREICDEKSLLLVEDCAEGFGTKWK
                     NSHVGTFGDVATFSFFGNKTITTGEGGMVLARNPQVMEKCRHLKSQGTSPTREYWHDAL
                     AYNYRMTNIQAAIGLSQIEMADEILSLKARTAASYASKLAGLPLRMHTPVGDVKHSYWM
                     CSIVLDNSEHREPLRQHLRENGVDTRPFFPPAHRMPHSASTGSYPVADSLSARGLNLPS
                     FPHITDVEISFVCDLVRSYFSNHSNHI"
     gene            7552..8334
                     /gene="wzm"
     CDS             7552..8334
                     /codon_start=1
                     /function="predicted integral membrane domains of
                     ATP-binding cassette (ABC) transporters"
                     /gene="wzm"
                     /note="Wzm"
                     /product="ABC transporter"
                     /protein_id="AAC98614.1"
                     /transl_table=11
                     /translation="MISYMANVWKVRHFWWHLSMSDLRGRFRRSSLGILWAVIQPLALT
                     LLLSFVFSKLLNQSISAYAPYILSGIIIWEYISFTVVGGSTALVQADAYIKQTRNPLAI
                     YTLRNTVSGLVVLSVASISLFGWVLIMFPENFSLSWLAIPTLLPILALIVWPLATIVGY
                     IGARFRDLPNALALVLQAAWFVSPVYFKESMFRQGGLNAFVDYNPIYHVMQILRAPVLY
                     GEWPTATNYIWCLGVSLLLTCVAVAVGMRAEKRAIFYL"
     gene            8331..9089
                     /gene="wzt"
     CDS             8331..9089
                     /codon_start=1
                     /function="predicted ATPase domain of ABC transporters"
                     /gene="wzt"
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 124; E-value:
                     1.3e-37)"
                     /gene_kind="transport"
                     /note="Wzt"
                     /product="ABC transporter"
                     /protein_id="AAC98615.1"
                     /transl_table=11
                     /translation="MIQPSITLSNVHLHYAASAFKERSVKTLVNALLSMRRSAGANIED
                     IHALKGISVDIARGERVALIGHNGAGKSTFLKTIAGLYPISSGTLKVTGTVRSLFDIGL
                     GFEPDATGRENILYRGLLLGLTPRFMREIEDEIIEFADLGDFIDYPIKTYSAGMQVRLA
                     FAISTAVDGDILLLDEVIGAGDAAFMTKAKARIMNMVEKAEIMVLASHDLANVRQLCTR
                     ALVFKAGTIAFDGRVEDAISFYNSGMGAIA"
     gene            9086..9940
                     /gene="wbkB"
     CDS             9086..9940
                     /codon_start=1
                     /gene="wbkB"
                     /product="WbkB"
                     /protein_id="AAC98616.1"
                     /transl_table=11
                     /translation="MIRDSGAPRRVLWLLNHTTLRECEVPLLQSMGFEIFTPKRFPRNS
                     DNRSASVSFETDESLTIPASVIDELNSYDSYQAPVNPRIDFLINYYFENAIVAYMFPMF
                     TQIISRFKGRILLRAFGLTSETWTYFDFANFVAGSFFKRTWMKASNQFWFAASYSSLIE
                     IEPNFIRERTVLLPVGLPERILAKQDTWRGGDKRIMFVCPDIETYPEAKAAYSESKHVF
                     GDLSHVICGNQTIPVVDDDNVVDRLSAEDGGRLPIAMSCTNCSANCTNVTQVGMQSRVS
                     IYA"
     gene            9967..10746
                     /gene="wbkC"
     CDS             9967..10746
                     /codon_start=1
                     /function="catalyzes the conversion of
                     GDP-4-NH2-4,6-dideoxymannose to
                     GDP-4-formamido-4,6-dideoxymannose"
                     /gene="wbkC"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1228:methionyl-tRNA formyltransferase (Score: 134.8;
                     E-value: 5.3e-41)"
                     /gene_kind="biosynthetic-additional"
                     /note="WbkC"
                     /product="formyl transferase"
                     /protein_id="AAC98617.1"
                     /transl_table=11
                     /translation="MAIAPNTRVLVAGYGLPAEFCVTTLIGMGVEIDKIAVATHREDNR
                     NCGLHSMLRLRNIQFTTAAANSEEFYEFGANFDPDMIISMHYRSLIPGRFLKLAKKGSV
                     NLHPSLLPAYRGTNSVAWVIINGESETGFSYHRMDENFDTGAILLQERISVEETDTAFS
                     LFHRQIARAMLRLEEVILKLDQGDPGFAQLGEASYYARELPFGGVIDPRWSEVQIDRFI
                     RAMFFPPFPPAVLKIDGKVYYVPSIDIYRSLMRGIPS"
     repeat_region   complement(10855..12130)
                     /rpt_family="ISBm2"
     CDS             complement(11082..11804)
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1026:transposase
                     (Score: 160.8; E-value: 6.7e-49)"
                     /gene_kind="other"
                     /product="P2 ISBm2"
                     /protein_id="AAC98621.1"
                     /transl_table=11
                     /translation="MVTPAASRKAVAHLMDHHQMSERRTCKAMGFCCMAIRYETRRSDD
                     HDLRERMKALAHERRCFGYRRLQVLLRREGHLVNHKWLFRLYREEKLTVRKRGGRKRAI
                     GTRAPMLIPPAANVRWLLDFVSDQLTDGRRFRVLAVVDDCTRECLPLVADTSLSGLRVA
                     RELDRIIERRGKPKMIVSDNGSEFTSNAILHWVDRTKMEWHYIAPGKPIQNAFIESFNG
                     QLRDELLNETFFSSLTHA"
     misc_feature    complement(11082..12022)
                     /note="contains putative ISBm2 transposase coding region;
                     -1 frameshifting may result in the fusion of two
                     overlapping ORFs (P1 and P2 ISBm2)"
     CDS             complement(11798..12022)
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1281:IS407A,
                     transposase OrfA (Score: 105.4; E-value: 2.1e-32)"
                     /gene_kind="other"
                     /product="P1 ISBm2"
                     /protein_id="AAC98619.1"
                     /transl_table=11
                     /translation="MKEHEAGTPVAELCRKHGVSDASIYKWKAKFGGMDVSEAKRLKTL
                     EDENTKLKRLLADAMLDNAALKDLLGKKW"
     misc_feature    complement(12461..12835)
                     /note="partial P2 ISBm3 coding region"
     misc_feature    complement(12461..13404)
                     /note="contains putative ISBm3 transposase coding region;
                     -1 frameshifting may result in the fusion of two
                     overlapping ORFs (P1 and P2 ISBm3)"
     repeat_region   complement(12470..13493)
                     /rpt_family="ISBm3"
     CDS             complement(12817..13404)
                     /codon_start=1
                     /product="P1 ISBm3"
                     /protein_id="AAC98622.1"
                     /transl_table=11
                     /translation="MNIHKNARLTPLRREEMAVAVFGGRLTKAQAARLYGVSLKIVSRW
                     TERFRISGRAAMTDRSSRPTRSPRQMGQDIVERILHLRRQRLTGKHIAMETGVSPATVS
                     RILRRAKLSRMKDIDPVEPVIRYEYAEPGGLIHLDIKRLGRFERVGHRITGNRTRQSNT
                     RGVGWEYVHVCIDDVSRIAFTDIFPDEKAIMP"
ORIGIN
        1 gaattctcga tcgtggaagt caatattgtt cccttgacta ccaggcgaag ctgaaggcga
       61 gcagcatcac gatttccatg tctggaacgg gaaattgcta cgataacgct atggttgaaa
      121 cattcttcaa gacattgaaa tcagagttga tctggaggac ggtaacggca gcagcgatgc
      181 atatgcttga gaagaaggtg tgagcgcatc ggtcatatct ggtgtgaatt cttctccagt
      241 ccttaagcct gccgaacata ttttcgatct tatgacgccg tttatagagg accggatcat
      301 atgggatgac atccttacgg ttggctcgtg atggaatgca tgcggtgatt ttacgctctg
      361 ccaatgcatc tcgaaaccag tcggcgtcat atcccttgtc tgccaacaga tcttttgctt
      421 tgggaaaggc gtggagcatt ttagctgctc ctttgtaatc gctcatctgc ccttcgctca
      481 gaagcaggat gagtggccga ccgtgaccgt cgcatacggc atgtagcttg gaattcaagc
      541 cacctttggt gcccccaata cgtcggggaa catccccttt tttagcagac tggctgcggt
      601 acggtgcgct ttcaggtggg tagcgtcgat catcaattga tccggcttac cgcgtttggc
      661 agccagttcg gccaagatcc ggttaaacac gccgagcctg ctccagcgga tgaaacggtt
      721 gtagatcgtc gtgtggggac cgtattccct cggggcgtcg cgccaccgaa gcccggtgcg
      781 cagcacaaag atgataccgc tcaaaatcaa gcgatcatcg acgcgtggaa ccccgtgaga
      841 taacggaaaa tagggcttga tccggcacac ctgtgcctgc gacagcaaca gcaaatcact
      901 catagcagaa gcctccttgc tatccgattg aatcaaaggc gaccgatatt cgcaagtaat
      961 ttaataggtc atgagcttag attctctggc tttgatgaaa acactttcca gcttttctat
     1021 actattatgc cagttgaaac gctcaatgtt ttgtaaagca agtaatgact tccgctgcca
     1081 tagtttatta tcagtagcga ttctataaag tgcattcgat atttcggcta cgttcagagg
     1141 atcgaccaag attgctgcat ccccagccac ctcaggcatc gatgaacagt ttgacgtaat
     1201 cacaggaact ccgtaggctt gtgcctcaat tatgggtaag ccaaaacctt cgtagagaga
     1261 cggcatgaga agcgtccgtg catttctata taaatcgttt agatcgctgt ctgtaacata
     1321 cccagttaaa cgcgtatact cagcaatgcc aagccgattt atagtcttcc caatatctcc
     1381 taggttccag ccctttccac cggctacaac cagcagaaaa ttatttcgaa cacattcagg
     1441 taagtttgca taagcttcga ggagccgtat aaggtttttt ctaggctcta aggtaccgat
     1501 gaacaagccg tacgacttgt taattcgatg ctcttttaag atgttcgaag taccgatcct
     1561 attgaagtgc gagacacccg gatataccat ttgaatcttt gaagtatact gagggaatac
     1621 cgaagaaatt gctgatgaag ttgcatatga tacagcaaca atttgatcag cattcctaag
     1681 cgctcctttc ataaggaagc gatctgcaag ccaaccttgc agtctcattg ttgacgatgc
     1741 gtagtaccaa accaagtcgt gaatactcac aacgctcgga atcgctccct tcaacgcagg
     1801 gaggcggtgg gcaggtcccc agaaaacatc aattcgatct tttctgagct gtagtggcaa
     1861 aacagtttga gaccaaaaaa gccgccccgc tggcccttga aaattactaa ctcgaaagtc
     1921 gaatccttct gaagaggata actgcacccg tggcttttct ggtgtataca agacaagctc
     1981 atgtcctcgc tcataaagct ctcggcaatt ttccatgaca tacctagata tgccagttat
     2041 attattaact aaattgcgag cgtcgacacc aattcgcatc actttacaca ttccttctat
     2101 gaagctaatt gtttgatccc ggcatttgat ggtgcggatt gagtttaaat cgttctggct
     2161 ctgttgccca gattttgcaa atgtattcgt agggagttaa ggcgtcgact cataaacttt
     2221 ggcaccatat tcttgttgcg acgagcttta cggctgcgag gtagttttcc gggcgcttat
     2281 catatcgggt tgcgatgtct cggaactgct tgatcctgtt gaagaagcgc tccacaagac
     2341 tgcgttgccg acagacccag cgcgaaaaca cgaaagagtc cttgcgattg ctcttcggtg
     2401 ggatgttggc ccaggccttt cgctcggcgg ccttagggcg tgtctgcatt taacgtaacc
     2461 agatcatagc gcatgcgaga tggacgaaac ccacgaatgc ggtcaatgtt ttctcgcatc
     2521 gcagcgcaat acgacgatag cgtttcaact tgttaaaaaa gcattcaatc tgatggcgtt
     2581 ccttgtacag cctccagtcg attgttggga cactggaacg tgttggattg accttgatct
     2641 gagccgttgc cttgagattg ctggcaatga aggcccttaa gtgatcggca tcataggctg
     2701 catcagcaat gacatgcccc acacccttca agccggatag aaggcttgaa gcttgcggac
     2761 agtcaccata atggccgggt gttggcttta ttcgcagcgg taggccgata gcatcgacaa
     2821 cagcatgcag cttggtcgtc aatccaccgc gcgagcgacc gatgcaggca gcttcagccc
     2881 cctttttgcg cccgccgcat ctgcgtggac tttcgatatg gtgctgtcaa tgaggacata
     2941 ttcaaagtcc ggcgtatcag ccagggcatg gaaaagcttt tcccatacac cggcgtgcga
     3001 ccagcgccga aagcgggcat gaaccgctgt ccatttgccg aaggtcgcag gcagatcgcg
     3061 ccagtgcgct gcattggcag ccatccacaa gatggcgtcg acaaataatc ggttatcaac
     3121 gccactgcgg ccgggcgtac caactcgccc cggaagatat gcttcgatcc ggttccattg
     3181 ctcatctgta aggcttcgtc tgctcacggc tgttctcctt taacaacctt gaatcagaat
     3241 ttcgtacaaa agggaatcct tgaatgcaga caagccctag gtgtttggtc ccatcatatg
     3301 atggtgtggt ttgacgataa aggcctcagg ctttgatttc cagagttcgt gaatcgcttc
     3361 atagggtgtt ttaaacttca aggctttgag ttgtttggca aagttgtatg cagtcagcca
     3421 atcgcgcaca tggcggcgca tttcgttaat cgagctgtag tgaaaggatt tgaccgtcgc
     3481 ttccttgatc gtccgcacca tccgctcggc ctgaccattg gtccacggat gatatggttt
     3541 tgtcagccgg tgctcaatgc cgtgttcaag agctactcgt ccaaagatgt acgagaaagt
     3601 aaggccagta ccttgctccc gctggacaaa ttgcacgcca ttatccgtca gaattgtatg
     3661 gatcctataa gggacggttc tgatcaatga cttgaggaac ccggcggggc cagttttgtg
     3721 gccttgcgat agatacgtgc gaacaccagt ttgcttgtcc gatcaacagc gacgaagaga
     3781 aagcccttgc cgccctcata gcggagctcg gcaatgtcaa tgtggaagta gccgatctcg
     3841 tagtctttga acttcttggg cttttcccgg tcgcctttcg gcagacgcga tataccgtgg
     3901 cgttgcaggc accggtgcaa cgaagaacat gtcaaatgcg ggatgacatc cttgagcgcg
     3961 atgtagacgt cgtcgagcgg taaccttgcc tggacccgca atgccacgat tgcagcctct
     4021 tccaccggag ccagcaccgt actgcgccgc tctttcggtc ccatcggcat atcttcgacg
     4081 gaacgcctct tgcgccattt caggaccgtt ttctcattga tgccgtaccg ccgcgcaagc
     4141 tccgcggccg aagctttcga tcgctgtaac tctgctcgaa tggcgtgcgt ggtcttggcg
     4201 ctgccgtgaa gaatttgtcc cgtagctctc tccgctgtgt cggcgacaac tctacaccat
     4261 cacatgctgg gaccaaacac ctagcccgaa tggcattcgt gtcatacccc ttgtcggcca
     4321 gcaggatgtc cccctcacca aactcattcg tcagcgcgtc ggcttcggca caatcggcga
     4381 tctgtccacc agtcagacgt agattgaccg ggcgaccttc ggcatcaacg agcgcgtgga
     4441 ttttgctcgt aagcccgccg cgggaacgtc ccatgccacc atcgtctcca tccccttttt
     4501 tcctgtggcc gcgtgctggt gaacgcggac acaggtcgag tcgatcatga cgatgtcgcc
     4561 atcgtaggcc ttgggcaccg tttcaaaaag ccgatcccag accccggctt tgcgccagcg
     4621 cacgaagcgg ttgtaacagg ccgcggcggg ccatagcgtt cgggaacttc cgcccagggc
     4681 gagcccgttc gaaatcgcca caggatgcca ttcagcaccc gccgatcatc aacgcgagca
     4741 acgccgcgtg gtttgttggg caacaacggc gatataatcg accattcgtg gtcagttagt
     4801 tcgtagcgac gacgcgtcat caaaggctcc cctatttcga agccttgaat catagcggcc
     4861 gataaatgcc aaaaaatttt atgctgtaat atgtgcatgt caaggtggac ttggttcgga
     4921 aaagaggaaa ggttttcatg agttgaaagg aaacctttat gacgaagcaa gtccaggccc
     4981 gaggaataat ccatgcgtgg catagccgac tcacttatcc aaccccttcc gtttcggatg
     5041 aggaatcaca agaacgcgat ggcagaagcc ctaaatgcgc cataattttt tcgcgttggt
     5101 atcaatgcca acactaatgc ttgactctaa tagctgaaca ttgcctaaaa ggtctcgtca
     5161 gcaaaatgcg tttaccggag gctcatgtta gatgacctcg tagctgaaac aagtagttca
     5221 gttttaaagc tatttcaatc gcgttcaaat acgtgtaatg ctccacgagc aagttgagat
     5281 cataggaagt tggccatgcg tttacagttg gcatggcaaa aacgggaatg aacaatcgtg
     5341 aagaaagtcg cacttatcac aggtatcacc ggccaagacg gcgcctacct tgcggaattg
     5401 cttctacaaa aaggttatgc agtacacggg attaagcgtc gcacctcgca atttaacaca
     5461 gcacgtatcg atcatctgta ccatgatccg cacgagcagg gtgtcgatct aacacttcat
     5521 catggcgatc taacagatac gtcaagcttg gttcgaatca tgcagctcgt tcggccggat
     5581 gaagtttata accttggcgc acaaagccat gttgctgttt catttgagga gccggagtat
     5641 actgcgaact cagacgcact aggtgccctt cgtctgctag aggcgatccg aattcttgga
     5701 atggagaagc agacccggta ctaccaagca tccacctctg agctttatgg tctggttcaa
     5761 gaaatcccgc aaaaagaaac gacgcctttt taccctcggt cgccctatgc cgcagcgaag
     5821 ctttatgcct attggattac ggtaaactac cgagaagcgt atggtatata tgcttgtaat
     5881 ggcatacttt ttaaccatga atctccactt agaggagaga cttttgtcac gagaaaaatt
     5941 actcgcgctt tagctagaat taagatcggt aatcagaatc gcttatactt agggaatttg
     6001 gactcattac gcgactgggg gcatgctcgg gactacgtcg agatgcagtg gttaatgctt
     6061 caacaggatc aaccagaaga ttttgtaata gcaactggta aacaatattc agttcgagaa
     6121 tttgtcacgc tcgctgctaa agacataggc attgagatgc gatgggaagg tagcggtgaa
     6181 gccgagaagg gttatgatgc caagaccggt gcatgtattg tcgaggtcga tcctagatac
     6241 tttcgaccag ccgaggtgga aactcttctg ggagatgcga ccaaggcgca tcaaaaactg
     6301 ggctggaagc cgaaaatctc ttttgagacg cttgtatctg agatgatagc agaagactta
     6361 gcggcggcgg ctcgtgagaa tttgctgcgc gaacatggtc accaattttt cgcccctaag
     6421 gaatgatcca tacatggata taccagttta ctctccctat ctctgtggga atttcaaaaa
     6481 gtatgtgaac gaatgccttg atacagggtg gatctcgtcg aggggtgaat tcatatctcg
     6541 ctttgaagat gcatttgcac aatatgtcga tgttccgtct gcggcttcag ttgcaaacgg
     6601 aacggtggca ctacatctcg ctctagatgc tctgggcatc ggggcgggcg atgaagtcat
     6661 tgtaccgacc tttacctata tcgcttcagt taacactatc ttgcaaaccg gggcaacacc
     6721 ggtttatgtg gactctctcg aaaatacatt gcagatagat ccagaggggg tgcgactggc
     6781 gattacagag cgcactaagg ctgtaatggt tgttcatctc tatgggcatc catgtgacat
     6841 ggattcgatc cgagagattt gtgacgaaaa atcgctactg ctcgtcgaag actgtgctga
     6901 aggattcgga actaaatgga aaaacagtca cgtcggcacg tttggcgacg tggcgacgtt
     6961 tagtttcttt gggaacaaga caattacgac cggtgaaggc gggatggtgc tagcgcgcaa
     7021 tcctcaagtc atggaaaaat gccgacatct caaaagtcaa ggtacttcgc ctacacgaga
     7081 atactggcat gatgcgcttg cgtataatta cagaatgaca aatattcaag cagcaatcgg
     7141 cctgtcgcaa attgaaatgg cagatgaaat actctccctt aaagccagga cagctgcctc
     7201 ttatgccagc aagttagctg gattgccgct tcgtatgcac acccctgtgg gagacgttaa
     7261 acattcatat tggatgtgct ctattgtact tgataactcg gaacacagag agccgctgcg
     7321 ccaacattta agggagaatg gtgtagatac acgaccgttt ttcccgccag cccatcgtat
     7381 gcctcacagc gcttccacag gatcttaccc tgttgctgat agcttatccg ctcgtgggtt
     7441 gaacctgcca agcttcccac acattactga tgtagaaatc agttttgttt gtgatttggt
     7501 caggagttat ttttctaatc attccaacca catttagtga gacgatttcg tatgatatcg
     7561 tatatggcta atgtctggaa ggtacgccac ttctggtggc acctttcaat gtctgattta
     7621 cgtgggcgct tcaggcggtc ctccttggga atattatggg cagttataca gccactagcg
     7681 ctcacgctgc tactgtcttt cgtgttttct aaattgttga atcaaagtat atctgcatat
     7741 gccccctata ttctatctgg gattattatc tgggaataca tatcatttac agtggttggt
     7801 ggctcaacag cgcttgtgca agccgatgca tatataaagc aaaccagaaa tcctcttgca
     7861 atttacacgc ttaggaacac tgtttctggc ttggtcgtat tatccgtagc aagtatctcc
     7921 ctattcgggt gggtacttat catgtttcct gaaaacttct cgctttcatg gttagcaata
     7981 ccaactttgc tacccatcct tgctttgata gtttggccgc ttgccacaat cgtcggctac
     8041 atcggcgcaa gatttcgaga tctgccgaat gctctggcgc tcgtgttaca ggcagcttgg
     8101 tttgtttcgc cggtctattt taaagaatcg atgttcaggc agggtggatt gaatgcattc
     8161 gttgattata accctattta ccacgtgatg cagattctaa gagcccctgt cctttatggg
     8221 gaatggccta cggctaccaa ttacatttgg tgcttaggtg tgagcctcct cctaacctgc
     8281 gtggcagtag ctgtggggat gcgtgcggag aagagagcca ttttttacct atgatccagc
     8341 catcgattac cctgtcaaat gttcatctgc actacgctgc atcagcgttc aaagaacgct
     8401 cagtcaaaac tctagtaaac gccttattga gtatgcggcg cagcgcagga gcaaacattg
     8461 aagacatcca tgccctaaag ggtatttctg tagatatagc gcggggcgaa cgcgttgccc
     8521 tgataggtca caacggggct ggcaaaagta cgttcttgaa aactatagcc ggtctctacc
     8581 ctatatcatc tgggacatta aaagtgacgg gtaccgtaag atccctgttc gatattggtc
     8641 ttgggtttga gcctgatgca actggccgtg agaatattct ttaccgtggg ttgcttctcg
     8701 gactaacgcc acgtttcatg cgagagatcg aggatgagat catcgagttc gcggatctcg
     8761 gcgattttat cgattatcca atcaaaactt attctgccgg catgcaagtt cggctcgcct
     8821 tcgcgatttc gacagcagtc gacggcgaca tactccttct agacgaagtt ataggtgcag
     8881 gtgatgcggc attcatgact aaggcgaagg cccgcataat gaatatggtc gagaaggctg
     8941 agataatggt tctagcaagc catgaccttg cgaacgtccg tcagctttgc acacgagcat
     9001 tggttttcaa agccggcaca attgcatttg atggcagggt agaagacgcg atttccttct
     9061 ataactcggg aatgggagct atagcatgat aagagattca ggcgctccaa gacgtgtttt
     9121 atggttgtta aatcatacta ctttacgaga atgtgaggtc cctcttcttc agagtatggg
     9181 gtttgagata ttcacgccta aacgttttcc tcggaattcc gataacagaa gcgcttcagt
     9241 tagtttcgaa acggatgaga gtctgacgat tccggcttct gtaattgacg aactaaactc
     9301 ttacgattcc taccaggcac cagttaaccc acgcattgat tttctgatta actattactt
     9361 tgagaacgcc attgttgcct atatgtttcc aatgttcacg cagataatat ctcgtttcaa
     9421 agggcgaata ttgttgagag cgtttggttt aacgtccgag acctggacct atttcgattt
     9481 tgctaatttt gtcgctggtt cattttttaa gcgtacatgg atgaaagctt ccaaccaatt
     9541 ttggtttgcg gcaagctatt ctagtcttat agagatagag ccaaatttca tacgagagcg
     9601 aactgtcctc ttgccggtag gcttaccgga acgtattctc gctaagcaag acacttggcg
     9661 aggtggcgac aaacgaataa tgtttgtctg ccccgatata gagacctatc ctgaagcaaa
     9721 agcggcctac tctgagtcta agcatgtatt cggcgacttg tcgcatgtta tttgtggcaa
     9781 tcaaacgatc ccggttgttg atgacgacaa tgtcgtagat cgactaagcg ccgaagatgg
     9841 tgggcgctta ccaatcgcga tgagctgtac gaattgcagc gcgaactgta cgaacgttac
     9901 ccaagttgga atgcagtcgc gggtgagtat ttacgcatag taactgacga aaaagaggca
     9961 ttttaaatgg cgattgcacc aaatacacgc gttttggtag caggttacgg attgccggcg
    10021 gaattttgcg ttacaacctt gattggcatg ggcgtagaga ttgataagat tgcggttgcg
    10081 acgcatagag aagacaatcg taattgtggt ttacattcca tgttaaggct taggaacatt
    10141 caattcacta ccgcagctgc aaattcggaa gaattctacg aatttggagc taatttcgat
    10201 ccggacatga ttataagcat gcactatcga tcgttaatcc cgggtcgctt cctaaaattg
    10261 gcaaaaaagg ggagcgtaaa tcttcatccg tcgcttcttc ctgcttatcg cggaacaaac
    10321 tcggttgcgt gggtgattat aaatggcgag agcgaaaccg gtttcagcta tcatcgtatg
    10381 gacgaaaatt tcgatacggg tgcaatattg ttgcaagagc gaatttcagt tgaagaaacc
    10441 gatacagcct tttctttatt tcaccgacaa attgcgcgcg cgatgctgcg cttagaggaa
    10501 gtcatcctta aactagatca aggagatccg ggatttgctc aattgggtga agctagctat
    10561 tacgcccgcg aacttccttt cggtggcgtt atcgacccaa gatggtcgga agtccagatt
    10621 gatcgtttta tccgagcaat gttctttcct ccgttcccac cggcagtatt gaagattgat
    10681 ggcaaggtat attatgttcc ttctatagat atttaccgct ctcttatgag gggtattccc
    10741 tcgtaaaatg gcttgttgct tgtttaggtt tttggtccca gcaggtgatg gtgtagtttg
    10801 atgataaagg cttctggctt tgatttccaa agttcctgaa tggcttcata gggtgtgacg
    10861 ttgcccccca agttttatcc agttttgagt tcgcgccagc ggttttgggt tgttgtattc
    10921 ggggcggtag cggcgggttg cggtgcggag tcgtccgggc tgcgcagcac cgcatcacgt
    10981 cgcgggttga ttgtctgagc gaactcggca ggcgtcatcc agccgagttc agagtgtggt
    11041 cgatgatcgt tgtaatcgct gcgccagttt aaaagcgctg atcatgcatg ggttagtgac
    11101 gagaagaacg tttcattcag gagctcgtct cgtagctgtc cattgaagct ttcgatgaag
    11161 gcgttctgga tcggtttgcc aggcgctata tagtgccatt ccattttggt ccgatccacc
    11221 caatgcagga ttgcgttact ggtgaactca ctgccgttgt cactgacgat catcttcggc
    11281 ttgccacgtc tttcgatgat ccggtccaac tcacgggcaa cccgcaagcc ggaaagggac
    11341 gtatcggcga caagaggcag gcattctctt gtgcaatcat cgacgaccgc aagcacccgg
    11401 aacctgcggc catcggtgag ctgatctgac acgaagtcca gcaaccagcg aacattggca
    11461 gccggcggga tcaacatcgg cgctcgggtg cctatcgccc gcttacgccc gccacgcttg
    11521 cgtaccgtca acttctcctc ccgatagagc cggaagagcc acttgtggtt cacaaggtga
    11581 ccctcacgcc tgagcagcac ttgaagacgt ctatatccaa agcagcgacg ttcatgcgct
    11641 agcgccttca tccgctcgcg aaggtcatgg tcatcgctgc gcctggtttc gtaacggatc
    11701 gccatacagc aaaagcccat ggctttacac gtccgccgtt cgctcatctg gtgatgatcc
    11761 atcagatgag cgacagcttt ccggcttgct gcgggtgtca ccacttcttt cccaaaaggt
    11821 ctttcaaagc ggcgttgtcg agcatcgcat ctgccaggag ccgtttcagc tttgtgtttt
    11881 cgtcttccag cgtcttcaac cgcttggcct cggacacgtc catgccgccg aacttggctt
    11941 tccatttata gatgctggca tcgctgacgc cgtgcttgcg acaaagctcc gcgacaggcg
    12001 tgcccgcctc gtgctccttc agaatgccga tgatctgttc cgtctgtaaa acggttgcac
    12061 ttcattctct ggtcctctca atgggccaga gcttacttca aaatggatta tttcaacggg
    12121 gcaaggtcaa aagtcaaaaa caccaatcag tcgtctcgct ctaggctcca gactcaattg
    12181 atccaccatg ttacgatcgc ggcgatgtag caggttgcgg cgaagttaat catgagcttg
    12241 tcatatcgtg tggcgatacg cctccaatcc ttgaggcggc agaaggtgcg ttcgatgata
    12301 tttctgcgtc ggtaggcgat cgggtcgaac ggtctgatcc gctttcgggt tggattgttg
    12361 ggaatgaccc gttgcgtgcc acgcgccgta aggaagtctc gcaaagcgtc gctgtcgtaa
    12421 gcggcatctg ccgcacaaag ccgtgtggga ggtaaaggct ctagctgtgg agcgtcaaca
    12481 ggttgtctcg gttgaggccg aggcgactga ttggtgtttt tgattttagc ccgctatgag
    12541 gtcgatgcca attgtacata tgtgtccagt ttggcagatg cgctttgcgg atctgtgacg
    12601 atgggtatgc acgtgcatag gcccactccc gtaatgcggt ctgaatgaag cgctcggcct
    12661 tgccgttggt ttttggcgta tagggcttgg ttcgaatgtg cttgagatcg agcgccttgc
    12721 aggccttggc gatatagcag gccccgttgt ccgtcatcac ccgcgtgacg gtgacgccaa
    12781 ggcttgcata gtaggcgacg gcagctttga gaacggctat ggcatgatgg ctttttcatc
    12841 cgggaatatg tcagtgaagg cgatgcggga aacatcatcg atgcagacat ggacatattc
    12901 ccagccaacc ccgcgcgtgt tgctttgtct cgtcctgttg cctgtgatcc gatggccgac
    12961 gcgctcgaaa cgcccaagcc gcttgatgtc gagatggatc aggcctcccg gctcggcata
    13021 ttcgtagcgg atgacgggtt cgaccgggtc gatgtctttc atccgcgaca gctttgcgcg
    13081 cctgagaatg cggctgacgg tggcgggaga aacgccggtt tccatggcga tatgcttacc
    13141 ggtcaggcgt tgtctgcgaa gatggagtat ccgttcgaca atgtcctggc ccatttgacg
    13201 ggggctgcgg gtggggcgcg acgagcgatc cgtcatcgcc gcccggccag atatcctgaa
    13261 ccgttcggtc caacgagaaa caattttcag cgagacccca taaaggcgtg cagcctgcgc
    13321 cttcgtcagc ctgccgccga aaactgcgac cgccatttct tctcgacgca atggcgtcag
    13381 tcgggcattc ttgtggatgt tcattcggag caatcctgga aaactgaggt gtggtaactc
    13441 cagtctcctc gatccgctcc gaatggacaa cctcctgaaa gctcacatct agcaggagaa
    13501 tgacaatggg ggcgtcgccg cgttgcccag gcgttattcg caacgcgact ggacggccgc
    13561 aactatcgac aacagcatgg atttttgtcg atctgccgcc gcgcgagcga ccgattgctt
    13621 cggcatcgcc cccttttccg ccagcggcag ggcggtgtgc ttttgcggtg gtgctatcga
    13681 tcatatggat gtcccgatcg gtcggttgca ccaaagcttc aaacagccgc cgccatattc
    13741 ctttggctga ccagcgatgg aagcgattgt agacagttgt tggaggtccg tagcaggctg
    13801 gacaatcctg ccatcggcaa ccggatcgag aattc
//
