LOCUS       BGC0000776             11821 bp    DNA     linear   BCT 24-JUL-2016
DEFINITION  Escherichia coli waa gene cluster encoding the LPS R1 core
            biosynthesis region.
ACCESSION   BGC0000776
VERSION     BGC0000776
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
COMMENT     ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2022-11-07 20:32:56
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 11821
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     gene            complement(1..1278)
                     /gene="waaA"
     CDS             complement(1..1278)
                     /codon_start=1
                     /db_xref="GOA:Q7AUZ4"
                     /db_xref="InterPro:IPR001296"
                     /db_xref="InterPro:IPR007507"
                     /db_xref="UniProtKB/TrEMBL:Q7AUZ4"
                     /gene="waaA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_1"
                     /gene_kind="biosynthetic-additional"
                     /product="KDO transferase"
                     /protein_id="CAD19780.2"
                     /sec_met_domain="Glycos_transf_1 (E-value: 5.4e-13,
                     bitscore: 38.5, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRHP
                     LKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQHV
                     YLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSAAGY
                     AKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLAAKAV
                     TLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPDAINLV
                     RQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGHNPLEAA
                     AHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRSFYGRHAV
                     EVLYQNQGALQRLLQLLEPYLPPKTH"
     source          1..11821
                     /db_xref="taxon:562"
                     /isolation_source="faecal"
                     /mol_type="genomic DNA"
                     /organism="Escherichia coli"
                     /serovar="O6:K5:H1"
                     /strain="DSM 6601"
     subregion       1..11821
                     /aStool="mibig"
                     /contig_edge="True"
                     /label="Saccharide"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..11821
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            1727..2749
                     /gene="waaQ"
     CDS             1727..2749
                     /codon_start=1
                     /db_xref="GOA:Q8KMX2"
                     /db_xref="InterPro:IPR002201"
                     /db_xref="InterPro:IPR011916"
                     /db_xref="UniProtKB/TrEMBL:Q8KMX2"
                     /gene="waaQ"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glyco_transf_9"
                     /gene_kind="biosynthetic-additional"
                     /product="heptosyl-III-transferase"
                     /protein_id="CAD19781.1"
                     /sec_met_domain="Glyco_transf_9 (E-value: 7.9e-66,
                     bitscore: 211.5, seeds: 18, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQDTIPILSENPEIN
                     ALYGIKNKKAKASEKIANFFYLIKVLRANKYDLIVNLTDQWMVAILVRLLNARVKISQD
                     YHHRQSAFWRNSFTHLVPLQGGNVVESNLSVLTPLGLESLVKQTTMSYPPASWKRMRRE
                     LDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEVVLTSGPDKDDLACVNE
                     IAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHIAAAVNTPLISLFGATDH
                     IFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAADVIAAVDKLLPSSTTGTSL
                     "
     gene            2746..3870
                     /gene="waaG"
     CDS             2746..3870
                     /codon_start=1
                     /db_xref="GOA:Q8KMX1"
                     /db_xref="InterPro:IPR001296"
                     /db_xref="UniProtKB/TrEMBL:Q8KMX1"
                     /gene="waaG"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1045:glycosyl transferase group 1 (Score: 124.4;
                     E-value: 1e-37)"
                     /gene_kind="biosynthetic-additional"
                     /product="UDP-glucose:(heptosyl)LPS
                     alpha-1,3-glucosyltransferase"
                     /protein_id="CAD19782.1"
                     /sec_met_domain="Glycos_transf_1 (E-value: 1.6e-38,
                     bitscore: 121.6, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGEC
                     PDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDRVVGFNKMPGLDVYYAADVCYA
                     EKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAERFH
                     ILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEALASLP
                     DSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLHPAYQE
                     AAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKALTQSSL
                     RQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            3863..4660
                     /gene="waaP"
     CDS             3863..4660
                     /codon_start=1
                     /db_xref="GOA:Q8KMX0"
                     /db_xref="InterPro:IPR010440"
                     /db_xref="InterPro:IPR011009"
                     /db_xref="UniProtKB/TrEMBL:Q8KMX0"
                     /function="unknown"
                     /gene="waaP"
                     /product="heptosyl-I-kinase"
                     /protein_id="CAD19783.1"
                     /transl_table=11
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAGK
                     SYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGINP
                     LTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINHRDC
                     YICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGLTQRD
                     IWRFMKVYFSAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            4676..5692
                     /gene="waaO"
     CDS             4676..5692
                     /codon_start=1
                     /db_xref="GOA:Q8KMW9"
                     /db_xref="InterPro:IPR002495"
                     /db_xref="InterPro:IPR013645"
                     /db_xref="UniProtKB/TrEMBL:Q8KMW9"
                     /gene="waaO"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glyco_transf_8"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glyco_transf_8C"
                     /gene_kind="biosynthetic-additional"
                     /product="UDP-glucose:(glucosyl)LPS
                     alpha-1,3-glucosyltransferase"
                     /protein_id="CAD19784.1"
                     /sec_met_domain="Glyco_transf_8 (E-value: 1.7e-62,
                     bitscore: 200.7, seeds: 67, tool: rule-based-clusters)"
                     /sec_met_domain="Glyco_transf_8C (E-value: 1.3e-26,
                     bitscore: 82.2, seeds: 14, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSAHYFNPQEMINKTIIFDERPAVSVASSFHVAYGIDQNFLFGCG
                     VSITSVLLHNNDVSFVFHVFIDDIPEADIQRLSQLAKSYHTCIQIHLVNCERLKALPTT
                     KNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTERDAN
                     WWSLRAQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAVVSRLTYMDQD
                     ILNLILLGKVKFIDAKYNTQFSLNYELKKSFICPINDETVLIHYVGPTKPWHYWAGYPS
                     AQPFIKGKEASPWKNEPLMRPMNSNYARYCAKHNFKQNKPINGIMNYIYYFYLKIIK"
     gene            5709..6704
                     /gene="waaT"
     CDS             5709..6704
                     /codon_start=1
                     /db_xref="GOA:Q8KMW8"
                     /db_xref="InterPro:IPR002495"
                     /db_xref="InterPro:IPR013645"
                     /db_xref="UniProtKB/TrEMBL:Q8KMW8"
                     /gene="waaT"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glyco_transf_8"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glyco_transf_8C"
                     /gene_kind="biosynthetic-additional"
                     /product="UDP-galactose:(glucosyl)LPS
                     alpha-1,2-galactosyltransferase"
                     /protein_id="CAD19785.1"
                     /sec_met_domain="Glyco_transf_8 (E-value: 1.9e-53,
                     bitscore: 171.0, seeds: 67, tool: rule-based-clusters)"
                     /sec_met_domain="Glyco_transf_8C (E-value: 2.7e-27,
                     bitscore: 84.5, seeds: 14, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNEFIKERFSYLADNKKENVPELNVSYGIDKNFLYGAGVSISSVL
                     INNSDINFVFHVFTDYVDDDYLKSFNETAKQFNTSIIVYLIDPKYFADLPTSQFWSYAT
                     YFRVLSFEYLSESISTLLYLDADVVCKGSLKPLTKIIFKDEFAAVIPDNDSTQAACAKR
                     LNIPGMNGRYFNAGVIYVNLKKWHEANLTPYLLTLLRGETKYGSLKYLDQDALNIAFNM
                     NNIYLAKDFDTIYTLKNELHDRSHRKYQQTITDKTVLIHYTGITKPWHSWAGYPSASYF
                     NIAREQSPWKKYPLKEARTVAEMQKQYKHLFAHGEYIKGITSLIKYKLKK"
     gene            6714..7406
                     /gene="waaY"
     CDS             6714..7406
                     /codon_start=1
                     /db_xref="GOA:Q8KMW7"
                     /db_xref="InterPro:IPR009330"
                     /db_xref="InterPro:IPR011009"
                     /db_xref="UniProtKB/TrEMBL:Q8KMW7"
                     /gene="waaY"
                     /product="heptosyl-II-kinase"
                     /protein_id="CAD19786.1"
                     /transl_table=11
                     /translation="MITSIRYRGFSFYYKDNDNKYKEIFDEILAYNFKTVKVLRNIDDT
                     KVSLIDTKYGRYVFKVFAPKTKRNERFLKSFVKGDYYQNLIVETDRVRSAGLTFPNDFY
                     FLAERKIFNYASVFIMLIEYVEGVELNDMPIIPEDVKAEIKASMEKLHALNMLSGDPHR
                     GNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEIKDYGYYSVIYRTKLRKF
                     IKKLKGKA"
     gene            7432..8460
                     /gene="waaW"
     CDS             7432..8460
                     /codon_start=1
                     /db_xref="GOA:Q8KMW6"
                     /db_xref="InterPro:IPR002495"
                     /db_xref="InterPro:IPR013645"
                     /db_xref="UniProtKB/TrEMBL:Q8KMW6"
                     /gene="waaW"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glyco_transf_8"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glyco_transf_8C"
                     /gene_kind="biosynthetic-additional"
                     /product="UDP-galactose:(galactosyl)LPS
                     alpha-1,2-galactosyltransferase"
                     /protein_id="CAD19787.1"
                     /sec_met_domain="Glyco_transf_8 (E-value: 3e-57, bitscore:
                     183.5, seeds: 67, tool: rule-based-clusters)"
                     /sec_met_domain="Glyco_transf_8C (E-value: 1.2e-25,
                     bitscore: 79.2, seeds: 14, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQSV
                     VMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVRIYKVSSAFIDIFPSLKQWSY
                     ATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDGDKFAAVIKDVPFMQEKP
                     AKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDILNIL
                     FFSHCIFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWTNYPCQ
                     KYFNEAYQVSCWNDVAFIPATNEKQYQVKYQHAKKNGDTFNAFIYFIKFKLNKYKRKLF
                     GQ"
     gene            8542..9525
                     /gene="waaV"
     CDS             8542..9525
                     /codon_start=1
                     /db_xref="GOA:Q8KMW5"
                     /db_xref="HSSP:P39621"
                     /db_xref="InterPro:IPR001173"
                     /db_xref="UniProtKB/TrEMBL:Q8KMW5"
                     /gene="waaV"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1123:polyprenol-monophosphomannose synthase ppm1
                     (Score: 66; E-value: 6.5e-20)"
                     /gene_kind="biosynthetic-additional"
                     /product="beta-1,3-glucosyltransferase"
                     /protein_id="CAD19788.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 3.2e-43,
                     bitscore: 137.1, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSNDYPLVSIIIPTYNSSDYITETLTKLEKQTYPNFEIVIVNDGS
                     KDNTSSVLREYGLTHSRLIIINKENGGVSSARNTGIRKAQGQFICFMDDDDEIDPNYLL
                     KMYSRQHETGGDAIYCGLYGHHIKNGVTYSPINTDFNEGSLLFDFFYKKVRFHIGCLFI
                     RKQLLEDNNLFFDEDLRLGEDLDFIYRLLITCDMYAVPYYMYKHNYRENSLMNSCRTIT
                     HYRHESFAHERIYSSVMQLYKGNRKEEIHTLLSKNRTYHKTRYLWNVLLNGDFELLNQL
                     VESNEKELKDCNLLGKRDKRRAKILASKNYILWRMVRLVNRKKNKR"
     gene            9571..10824
                     /gene="waaL"
     CDS             9571..10824
                     /codon_start=1
                     /db_xref="GOA:Q8KMW4"
                     /db_xref="InterPro:IPR007016"
                     /db_xref="UniProtKB/TrEMBL:Q8KMW4"
                     /gene="waaL"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Wzy_C"
                     /gene_kind="biosynthetic-additional"
                     /product="putative lipid A-core surface polymer ligase"
                     /protein_id="CAD19789.1"
                     /sec_met_domain="Wzy_C (E-value: 2.7e-12, bitscore: 36.5,
                     seeds: 81, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSFCWNEINSGVKSLILILCMLSLMTLSLWDDVATKFLHAAGIIS
                     ALYFLATPKKTITNNPTLLIFISLCLLGIVNIIWYSHYKVSGSVYTNAYRGPMETGKIA
                     LCSAFIFLVLFAKDEMRTKIKFGKLILFASLATQLLFFAHAMWQHFYLNVDRVALSASH
                     ATTAGYIILFPSLLASILILKSDFRHKTTLYTINFMLSLCAVIVTETRAAILVFPFFAL
                     LLIVMDSYINKRINYKLYCFIAIALLAGVFSFKDTLLTRMNDLNRDLVNYSHDNTRTSV
                     GARLAMYEVGLKTYSPIGQSLEKRAEKIHELEEKEPRLSGALPFVDSHLHNDLIDTLST
                     RGIPGVALTILAFSAIFIYALRTAKEPYILILLFSLLVVGLSDVILFSKPVPTAVFVTI
                     ILLCAYFKVQSDQCLLDK"
     CDS             complement(10894..>11821)
                     /codon_start=2
                     /db_xref="GOA:Q8KMW3"
                     /db_xref="InterPro:IPR002201"
                     /db_xref="InterPro:IPR011908"
                     /db_xref="UniProtKB/TrEMBL:Q8KMW3"
                     /gene="waaC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glyco_transf_9"
                     /gene_kind="biosynthetic-additional"
                     /product="heptosyl-I-transferase"
                     /protein_id="CAD19790.2"
                     /sec_met_domain="Glyco_transf_9 (E-value: 1.5e-87,
                     bitscore: 282.6, seeds: 18, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="PALTDAQQAIPGIKFDWVVEEGFAQIPSWHAAVERVIPVAIRRWR
                     KAWFSAPIKAERKAFREALQAKNYDAVIDAQGLVKSAALVTRLAHGVKHGMDWQTAREP
                     LASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQGDYAIAQHFLTNLPTDAGEYA
                     VFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWGAPHEEERAKRLAEGFAYVEVLP
                     KMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNITVYGPTDPGLIGGYGKNQMVCRA
                     PGNELSQLTANAVKQFIEENAEKAAMI"
     gene            complement(10894..>11821)
                     /gene="waaC"
ORIGIN
        1 tcaatgcgtt ttcggtggca ggtaaggttc cagcagttga agcagacgct gtagtgcgcc
       61 ctggttttga tacagcactt caacggcatg acggccatag aaactacggt aatcggcgtc
      121 ggtgagtaaa gaggaaacct cttttgcaag cgtagtggca tcggtaacgg taatcagccc
      181 gcttgcctgc tccagccgcg cgcaaatgtc tttaaagtta aacgtatgcg gccccatcaa
      241 taccggaata gcgtgtgcgg ccgcttccag cggattatgc ccaccacgtt caaccagtga
      301 accgccaaca aaggcgagat cggcaatgcc gtacagtaac atcaactcgc ccatcgtatc
      361 gccaaccaca acctgcgtgc tggtggaggg gacttcccct gaagatcgtg tgatatagct
      421 tagtccagcc tggcggacaa ggttaatcgc atccgggaag cgttctggat gacggggtac
      481 caggatgagc aataaattcg ggaattgctg taacaatgcc tgatgtgcgg cgatcaccac
      541 actctcttcg ccttcatgag tgctggtggc aatccatacc gggcggtgtg gtgcccattg
      601 gcggcgcagc gtcacggctt tagcagccaa ctgcggcgtt acagaaatat cgaatttcag
      661 gctaccggta acggtcacct gattattttt tgcgcccaac gccacaaaac gagcaccatc
      721 ttcttcattt tgggcggcaa tcagcgtaat acgacgcagc aagcgacgga cgaatttacc
      781 cagtttggca taacctgcgg ccgagcgggc agagagtcgc gcgttagcga tcaccagcgg
      841 aattttacgt ttatgtagcg ccgcaatcag gttaggccat agttcggttt ccataatcaa
      901 caccagttta gggtcgactt tattcaggaa acggttgagt gcatcgggca ggtcatacgg
      961 cagataaacg tgctgaacat ccttcccgaa agccgattgt acgcgctccg aaccggttgg
     1021 agtcatggtt gttacggtaa tcggtaaatc aggataacga tgacgcagtg cgcgcaccaa
     1081 cgggatcgcc gccagagttt caccgacgga gacggagtgc agcataatgc cgcctggttt
     1141 tagcggatgg cggtaaaaac cgtaacgttc accccagcgt tttcgatagg ccggagcctt
     1201 acgtccgcgc acccagagcc gtatccagat cagcggctga ataaggtaga gaagggcggt
     1261 gtaaagcaat tcgagcatag taaatagctg acttatggat gtgctgggga ttctatgtat
     1321 ttagctgtgg ctttaccatt acttttccca tttttgactt aaatagcaat ttacccgcca
     1381 attcttgctg aacatctata ctttttttgt atttttatgc gattcattga tttctgcagt
     1441 acgtgcagga aggaatcgca gagcaacagt aaaagaatat ggttgctaat gattttattt
     1501 gattgatttc tttattgaaa atcaacaatg tgcttaaaaa tcattcgagt cggattgccg
     1561 cgaaagaagt gatacactag cgcgtcaata tcagtgcggt atcagtattc aggtagctgt
     1621 tgagcctggg gcggtagcgt gctttttctg cttcacctta atcggataat ctcaacaaaa
     1681 agagttactt gtggataagc catttcgaag aattttgctc attaagatgc gttttcatgg
     1741 ggatatgtta ttaactactc ccgtcattag ttcgctgaaa aaaaattacc ctgacgcaaa
     1801 aatcgatgtg ctgctttatc aggacaccat cccgatcctg tctgaaaatc cagagattaa
     1861 cgcgctctac ggcataaaaa ataaaaaagc aaaagcctca gaaaaaattg ccaacttttt
     1921 ttatctcatc aaggtattac gtgccaataa gtatgacctt atcgttaatc ttaccgatca
     1981 atggatggtt gctatactgg ttcgcttatt aaatgcccgt gtgaaaattt cccaggatta
     2041 tcatcatcgg cagtctgctt tttggcgtaa tagtttcact catttggtgc cgttgcaggg
     2101 tggaaatgtg gtggaaagta acttatccgt gctgacgcca ttgggacttg aatcgttggt
     2161 gaagcagaca accatgagtt acccgcctgc aagctggaaa cgtatgcgtc gcgaacttga
     2221 tcacgctggt gttggacaaa attatgtggt tatccaacct acggcgcggc aaatcttcaa
     2281 atgttgggac aacgccaagt tttccgctgt gattgatgcc ttacatgctc gtggttatga
     2341 agtcgttctg acgtccggcc cagataaaga cgatctggcc tgcgtcaatg aaattgcgca
     2401 gggatgccag acgccaccag taacggcgct ggccggaaag gtgaccttcc cggaacttgg
     2461 tgcgttaatc gatcatgcgc agctgtttat tggcgttgat tccgcaccgg cgcatattgc
     2521 cgctgcagtt aatacgccgc tgatatcgct gtttggtgcg acagaccata ttttctggcg
     2581 tccctggtca aataacatga ttcaattctg ggcgggagat taccgggaaa tgccaacgcg
     2641 cgatcagcgt gaccgaaatg agatgtatct ttcggttatt ccggcggcag atgtcattgc
     2701 agctgtcgat aaattacttc cctcctccac gacaggtacg tcgttatgat cgttgctttt
     2761 tgtttatata aatattttcc ctttggcggt ttgcagcgcg attttatgcg tattgctcag
     2821 acagttgccg cccgaggtca tcatgttcgg gtttataccc agtcgtggga aggcgaatgc
     2881 cctgatgtat ttgaactgat caaagtgccg gttaaatcgc ataccaatca cgggcgcaat
     2941 gcggaatatt ttgcctgggt gcaaaaacat ttacgtgaac accccgtcga tagagtcgtt
     3001 ggatttaata aaatgccggg gctggacgtt tattatgccg ctgatgtttg ttatgccgag
     3061 aaagtagcgc aggagaaagg ctttttctat cgcctgacgt cacgttatcg ccattatgcc
     3121 gcctttgagc gggcaacctt cgaacagggc aagccgacac agctgctgat gctgacagat
     3181 aagcaaatcg ccgatttcca gaaacattat cagactgaag cggagcgttt tcatattctg
     3241 cctccgggga tttatcctga tcgtaaatat agccagcagc cagccaatag ccgtgaaatc
     3301 ttccgtaaga agaatggaat aaccgaacaa caatatttat tgttgcaggt tggttcagac
     3361 ttcacgcgta aaggtgtcga tcgttccatt gaagcacttg cttcgttacc ggattcgctg
     3421 cgccacaaca cattactata tgttgttggg caggataaac cgcgaaaatt tgaggcgctg
     3481 gcagaaaaac gcggcgtgcg cagtaatgtt cacttcttct cggggcgcaa cgatgtctcg
     3541 gaattaatgg cggcggcgga tttattactt catcctgcct accaggaagc cgcgggaatt
     3601 gtactgctgg aagcgataac tgcaggatta ccggtactaa caacagccgt ttgtggctat
     3661 gcgcattata ttgtcgacgc caattgcggc gaggctattg ctgagccatt ccgccaggaa
     3721 acattgaatg agattttacg caaagcgtta acgcaatctt cattgcgcca ggcctgggcg
     3781 gaaaatgcgc gacattatgc tgatacacaa gatttataca gtctgccaga gaaagcggcg
     3841 gacatcataa cgggtggtct ggatggttga acttaaagag ccgtttgcca cgttatggcg
     3901 cggtaaagat ccttttgagg aagttaaaac cttgcagggt gaggtatttc gtgaactgga
     3961 aactcgccgc actctgcgct ttgaaatggc gggcaaaagc tattttctca aatggcatcg
     4021 cggcacgacc ctgaaagaga taatcaaaaa tttactctca ttgcggatgc cagtattagg
     4081 cgcagaccgc gaatggaatg cgattcatcg actgcgggat gtcggcgttg atactatgta
     4141 tggggtggca ttcggcgaaa aaggcattaa tccgctgacc agaacctcgt ttattattac
     4201 cgaagatctg acaccaacca tcagtctgga agattactgt gctgactggg cgaccaaccc
     4261 accagatgtt cgcgtaaagc gtatgcttat taagcgtgtc gcgacgatgg tgcgcgatat
     4321 gcatgctgcg ggcattaacc accgtgactg ttatatctgt catttcctgc tgcacttgcc
     4381 tttttccggt aaggaagagg agttaaaaat ttcagtaatt gacctgcacc gggcgcagct
     4441 tcgcacgcgc gttccacgtc gttggcgcga taaagatctt attggacttt atttttcttc
     4501 gatgaatatt ggcctgactc aacgggatat ctggcggttc atgaaggtgt atttttctgc
     4561 cccgcttaaa gacattctca agcaggaaca agggctgctg tcgcaagcag aagcaaaagc
     4621 cacaaaaatc agggaaagaa cgattcgaaa atcgttgtaa tttccggaga gaaatatgag
     4681 tgcccactat tttaatccac aagagatgat caataagaca atcatcttcg atgaaaggcc
     4741 agcggtgtca gtggcatcat cattccatgt tgcttatggc attgatcaga actttctttt
     4801 tggttgtggt gtttcaatca cgtcagtttt gttacataac aacgacgtga gttttgtttt
     4861 ccacgttttt attgatgata tccctgaagc cgatatccag cgtttatccc aattagcgaa
     4921 aagctatcat acctgtatcc agatccatct ggtgaattgt gaacggctta aggcattacc
     4981 gacgaccaaa aattggtcta ttgccatgta tttccgtttt gtaattgcag attactttat
     5041 tgatcaacaa gataagatcc tgtacctgga tgctgatatc gcctgtcagg gaaacttaaa
     5101 gccgctgata acaatggatc ttgccaataa cgttgctgct gttgttactg aacgcgatgc
     5161 taactggtgg tcattacgag ctcaaagtct gcagtgtaat gaacttgaaa agggttactt
     5221 taattcaggt gtcctgttaa ttaatacact agcgtgggcg caggagtccg tttctgctaa
     5281 agcgatgtcg atgcttgctg ataaagccgt cgtttcccgt ttaacctata tggatcaaga
     5341 tatacttaat cttatcctgt tagggaaagt taaattcatt gatgctaaat acaatacgca
     5401 atttagttta aattatgaat taaaaaaatc atttatttgt ccaattaatg atgaaaccgt
     5461 attaattcat tatgtcggcc cgacaaaacc ctggcattac tgggccggtt atccaagcgc
     5521 gcagccattt atcaaaggca aagaagcatc gccctggaaa aatgaaccgt taatgcggcc
     5581 aatgaactca aattatgctc gttattgcgc taagcataat tttaaacaaa ataaaccaat
     5641 taacgggata atgaattata tttattattt ttatttaaag ataataaaat gacattgaga
     5701 aaagttaaat gaatgaattt ataaaagaac ggttttcgta tttagcagat aataaaaaag
     5761 aaaacgtccc agagctaaat gtttcctacg gtatcgataa gaattttttg tatggtgctg
     5821 gcgtttcaat ttcttccgtt ttgattaata attcagatat taattttgtc tttcatgttt
     5881 tcactgatta tgtggatgat gattatttaa agtcatttaa tgaaacagca aaacaattta
     5941 atacctcaat tattgtatat ttaattgacc caaaatactt tgctgatctg ccgacgtcac
     6001 agttttggtc gtacgcgaca tacttcaggg tattgtcctt tgaatatctg agtgaaagta
     6061 tttccacact gctgtatctg gatgccgatg tcgtttgtaa aggaagtctg aaacctctca
     6121 caaaaattat atttaaagat gagtttgctg cggttattcc tgacaatgat agtactcagg
     6181 cggcatgtgc aaaacgtctt aacattcccg gaatgaatgg acgttatttc aatgcaggcg
     6241 ttatctatgt caatcttaaa aaatggcatg aagcaaattt gacaccgtat ttactcacgc
     6301 ttttacgagg ggaaactaaa tatggctctc ttaaatattt agatcaggat gcgttgaata
     6361 tcgcatttaa tatgaataat atctacctcg cgaaggattt tgatactatt tataccctga
     6421 aaaacgaact tcatgatcgt agtcatcgaa agtatcagca aaccattacc gataaaacag
     6481 tattgattca ctatacaggg ataactaaac catggcatag ctgggctgga tatccgtctg
     6541 catcatactt taatatcgcg cgtgaacaat ctccctggaa gaaatatcct cttaaagagg
     6601 cgcggactgt tgcagaaatg cagaaacaat ataagcatct gtttgcccat ggtgagtata
     6661 ttaaaggtat aacttcatta attaagtaca agcttaagaa ataaattact cttatgatta
     6721 caagtatacg ctatcgcggc ttctcatttt attacaaaga taacgataat aaatataaag
     6781 aaatctttga tgaaatactg gcctataatt ttaaaacagt aaaagtatta cgtaatattg
     6841 atgataccaa agtatcgttg attgatacga aatatggtcg ctatgttttt aaagtctttg
     6901 ccccgaaaac gaaaaggaat gaacgttttt taaaatcttt tgtaaaaggt gactactatc
     6961 agaatttgat cgttgaaacc gatcgtgtca gaagtgcagg tcttactttt cctaatgatt
     7021 tttattttct ggctgagcgt aaaattttca actatgccag tgtttttatc atgcttatcg
     7081 agtatgtcga gggcgtagag ctcaatgata tgccgattat tccagaagat gttaaagcag
     7141 aaattaaggc ttcaatggaa aaattgcatg ccttaaatat gctatcgggc gacccacacc
     7201 ggggtaactt tatcgttagt aaagatggtg tcaggataat tgacctatcc ggtaaaagct
     7261 gtacggcaga gcggaaagcc cgtgatcgtc tggccatgga acgtcacctg ggtattgcca
     7321 acgaaataaa agattatggc tactattcag taatctacag aaccaaacta cgcaaattta
     7381 ttaaaaaatt aaaaggcaaa gcgtaaacca cacagtcaaa acggaaccaa catggattta
     7441 ttagctgaga gtattactga agtcgctgtc tctggggaaa ttgctaacac cgatcgtgtg
     7501 ttaaatatcg cttacggtat tgaccgcaac tttttatttg gtgcggcagt atctatgcaa
     7561 tcagttgtta tgcataaccc ggaccttgcg gttaagtttc atctctttac tgactacatt
     7621 gatgaagatt atctacaacg tgttaatgct tttaccagca aaaatgctaa cgttgaagta
     7681 agaatttata aagtatccag tgcctttatt gatatcttcc ccagcctgaa acagtggtct
     7741 tatgcaacat tcttccgttt agttgcgttc cagtatctga gtgaaactat tgaaaatctg
     7801 ttatatatcg atgctgatgt catctgtaaa ggctcattag ctggattgct tgatattaat
     7861 tttgatggcg ataagttcgc ggctgttatt aaagatgtgc cttttatgca ggaaaaacca
     7921 gcgaagcgtc tggctataga gggacttcca gggaattatt tcaacgccgg tgtagtatat
     7981 ctgcagcttg aagcatgggc gaaaaatgat tttatgaata aagccattgc tatgctggca
     8041 agtgacccgc agcacacgaa atataaatgc cttgatcagg atattttaaa tattctgttc
     8101 tttagtcatt gtatttttat tagcggcgat tatgattgct tttatggcat tgactatgag
     8161 ttaaaaaata aaagcgatga agattataaa aagaccatta ccgatgatac taagctgatt
     8221 cattatgttg gcgtaacgaa gccctggaac gactggacga attatccctg ccagaagtat
     8281 tttaatgagg cttatcaggt ttcttgctgg aatgatgtgg cgtttattcc agccacgaat
     8341 gaaaagcagt atcaagtgaa ataccaacat gcaaagaaaa atggtgatac gtttaacgct
     8401 tttatttact tcattaaatt caaattaaat aagtataaaa gaaaactatt tgggcaataa
     8461 taaaaatcat ggatgtctga gttacaataa taattaagga tgagtactat aatattttat
     8521 tagttcaggc aagagaacac gatgagcaat gattaccctt tagtatccat aataataccg
     8581 acgtataatt catctgatta cattactgaa actctaacga aattagaaaa acaaacttac
     8641 ccaaattttg aaattgttat tgttaatgat ggttctaaag ataacacatc aagtgttttg
     8701 agagagtatg ggttaaccca ctctcgatta attattatca ataaagaaaa tggcggtgtt
     8761 tcgtctgcca ggaatacagg tatccgcaag gcgcaaggac agtttatatg ttttatggat
     8821 gatgatgatg agatagatcc taactatctg ctgaagatgt attccagaca acatgagacg
     8881 ggaggggatg cgatttattg tgggctttat ggccatcata taaaaaatgg tgttacttac
     8941 tcacctataa atacagattt taatgaaggg tctttacttt tcgatttttt ttataaaaag
     9001 gtcagattcc atataggatg cttgtttata agaaaacaac tcctagaaga taataatctt
     9061 ttttttgatg aagatttacg actaggagaa gatctggatt ttatctatcg actgctaatt
     9121 acatgcgata tgtatgcggt tccatattat atgtataagc ataactatag agaaaattcc
     9181 ttaatgaact catgtagaac catcactcat tatcgacatg agtcatttgc gcacgaaaga
     9241 atctactctt ctgtgatgca gttatacaaa ggtaaccgga aagaagaaat tcatacatta
     9301 ttgagtaaaa atagaactta tcataaaact cgttatttat ggaatgttct acttaatggc
     9361 gattttgaat tattaaatca attagttgaa agcaatgaaa aagaattgaa agattgtaat
     9421 cttcttggca agagagataa gaggcgagca aagatattag catcaaaaaa ctatattctc
     9481 tggaggatgg taagactggt aaatagaaaa aagaataaac gttaggtctt gcattaaata
     9541 acccatcctc gtagcatagg ttgaaattat atgtcgtttt gttggaatga aattaactct
     9601 ggtgtcaagt ctttaattct catattatgt atgttgtctt taatgacttt gtctttgtgg
     9661 gatgatgttg caacaaagtt tcttcatgca gctggaatta tatctgcatt atattttcta
     9721 gcgacaccaa aaaaaacaat aactaataat cctactttgt taattttcat ctcattatgt
     9781 cttttgggta tcgttaatat catctggtat tcacattata aagtttcagg ctcggtttat
     9841 accaatgcat atcgtggccc aatggaaact ggaaaaattg ccttgtgtag tgcttttatt
     9901 ttcttagttc tttttgctaa agatgagatg agaaccaaaa taaaatttgg gaaactaatt
     9961 ctgttcgcat ccttggcaac gcagttactt ttttttgcgc atgccatgtg gcaacatttc
    10021 tatttaaacg tcgaccgtgt tgcattatca gcttcccacg ctacaacagc aggctacatc
    10081 atccttttcc cttctttact ggcatcaatt ctcattttaa aatccgactt tagacataaa
    10141 acaacattat atacaattaa cttcatgctt agcttatgtg ctgttatagt aactgaaacg
    10201 cgtgcagcca tattagtgtt tccattcttt gcgttactat taatcgtaat ggatagttat
    10261 attaataagc gaattaatta taagttatat tgttttattg cgattgcatt attagcaggt
    10321 gtattttctt ttaaagatac attacttacg agaatgaatg acttaaatag ggatttagtt
    10381 aattattcgc atgataacac cagaacttca gtcggtgccc gtctggcaat gtatgaagtt
    10441 ggtttaaaaa catattctcc aataggacaa tcactggaaa aacgtgcgga aaaaatacat
    10501 gaactagaag agaaagagcc tagattaagt ggtgctttac cctttgtaga ttctcatttg
    10561 cataacgatc tcatagatac gttatcaacg cgtggtattc ctggagtagc attaacaatt
    10621 ttagcctttt cagcaatatt catatatgcc ttaagaactg ctaaagaacc ttatatttta
    10681 atcttgcttt tttcactact ggtagtaggc ctaagtgatg taatactctt ttctaaaccg
    10741 gttccgactg ctgtgtttgt caccataata ttgctttgtg cttattttaa agtacaatcg
    10801 gaccaatgtt tattagataa gtaatgaata tgtgaaataa aatcagcaag gaagctgatt
    10861 ttatttcaaa attatctatt aaagagttgt aatttaaatc atggcagctt tttcagcgtt
    10921 ttcttcaatg aattgcttaa cagcatttgc tgtcaattga gacaactcat tccccggagc
    10981 cctacaaacc atctgattct tcccataccc accaattaat cccggatcgg taggtccata
    11041 aaccgtgata ttgggtctat ccagtgccgc cgttaaatgg cttaaccccg tatccaccga
    11101 cactacaaat ttagccccgg ccagtacgcg ggcaacgcct tccagactca tcttcggcaa
    11161 tacttcaaca taagcaaatc cttccgccag tcgtttcgcc cgttcttcct catgcggcgc
    11221 gccccacgga agtttaatcc gtattcctga atcagccagt aaaccaatca attctcgcca
    11281 gtgttcttcc ggccagtgtt tatcatctcg ggttgttgca tgaagaaata cggcatattc
    11341 gccagcatct gtaggcaggt tcgtcaggaa atgctgtgcg atagcataat cgccctgggt
    11401 ttgtggtttg ctatagccca gacttttggc aaacagttcg cgggtgcgtt ctacggcgtg
    11461 ctgctgtttt gcaatatgat gtttacgatt gtaaaacagg ctggctaacg gttcgcgagc
    11521 ggtttgccag tccattccat gctttacgcc atgcgccaga cgcgtcacca gcgccgcgct
    11581 ttttaccagc ccctgggcgt cgataactgc gtcatagttc tttgcttgta gcgcttcacg
    11641 aaacgctttg cgttcagctt ttatgggagc cgagaaccag gctttacgcc agcgacgtat
    11701 tgccacagga ataactcgct caacggcagc gtgccaggaa ggaatctgtg cgaacccttc
    11761 ttccaccacc cagtcaaact taatccctgg gattgcctgc tgggcatcag tgagtgcggg
    11821 c
//
