LOCUS       BGC0000777             11312 bp    DNA     linear   BCT 15-APR-2005
DEFINITION  Escherichia coli wbb gene cluster encoding O6 LPS side chain
            biosynthesis region.
ACCESSION   BGC0000777
VERSION     BGC0000777
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-07 20:33:10
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 11312
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..11312
                     /db_xref="taxon:562"
                     /isolation_source="faecal"
                     /mol_type="genomic DNA"
                     /organism="Escherichia coli"
                     /serotype="O6:K5:H1"
                     /strain="DSM 6601"
     subregion       1..11312
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000777"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..11312
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            357..1613
                     /gene="wzx"
     CDS             357..1613
                     /codon_start=1
                     /db_xref="GOA:Q8KMW2"
                     /db_xref="InterPro:IPR002797"
                     /db_xref="UniProtKB/TrEMBL:Q8KMW2"
                     /gene="wzx"
                     /product="putative O antigen flippase"
                     /protein_id="CAD19791.1"
                     /transl_table=11
                     /translation="MNRIQRVHNYLRLKDKIKNVFLGLYILQLFNYIAPLVIIPILIKY
                     IGLGEYGELVYITSIYQIVALIIDFGFTYTGPVVAARHRCETQNLQRYYSIVVLLKSLL
                     FIIALTCVFLLCRLNIVHLSFFGFLSIFLCTIGNILSPNWFLQGIGDFKKLSYSQVIVR
                     ITLFIILLVYVCSGGDNVFILSFLQNATLLICCIYLWPNIHISHVVHLKPNECIVEFKK
                     AGNVFIGVIGTIGYNGLIPVLIGNLCGNTSLGVFSIVQKMTTACQSLINPISQYMLSQV
                     SEIKPQDKLFYYRIKKSFFVHLTISIIACLCYMGLGQYVATFIGKVDVSFVIILFASII
                     TIFSSLNNVLGIQFLIPTDNVKILRSINVMAGIIVVSLSWLLISRFDILGGVLLNLIGE
                     FLVFSMLAFIAHRKWGARV"
     gene            1613..2956
                     /gene="wzy"
                     /pseudo=""
     CDS             1613..2956
                     /codon_start=1
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /gene="wzy"
                     /note="non-functional due to internal stop codon; homology
                     to O antigen polymerase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MKVKAVPAITFYLSLMLTILVLLFGNEPNKSQYILVIATITVFYI
                     AYITNKITSPASLLVISSFVFLGCRPLLSLFANYDYRIADWFIEGYMDDDVILANYAIT
                     LMYYGYTLGLILCKNTEKFYPHGPYPEKQLLKIKFLLTLFFLGSIGMVVKGIFFFNFIE
                     SNSYVDIYQSNITTPIGYDFLSYLFYCSFFLICAFHIQFRTNKKFLFIAICIAAFSTLK
                     GSRSEAITFLLTVTCIYFNEVKTRNLRLLITMIFVFSVIFVISEFISMWRTGGSFFQLM
                     QGNNPVINFVYGMGVSYLSIYQSVKLQLLSGGYNVTYLFSQLIITCS"
     CDS             2962..3822
                     /codon_start=1
                     /db_xref="GOA:Q8KMW1"
                     /db_xref="InterPro:IPR001173"
                     /db_xref="UniProtKB/TrEMBL:Q8KMW1"
                     /product="putative glycosyltransferase"
                     /protein_id="CAD19793.1"
                     /transl_table=11
                     /translation="MSEKNVSIIIPSYNRAHILKEVIPSYFQDETLEVIVINDGSTDNT
                     NSVLAELKEKYSQLVILENETNKKQMYSKNRGIEIAKGKYIFFGDDDSYLLPGVISRLL
                     ATKYETGADVIGARILYMNNNEKTIEDCINRHKKEGRFVSDLNRLDFSYTCDLDHPIEC
                     FYAQPFVLAERELISKYRFDISYTGNCYREETDFMLSLFIKNKKFIYDSKALLINLPPR
                     KATGGARTANRLKYHYESCINNYRFLKKYNDNLNLLSGQKHAIFYRQCQFVLLKMKSFI
                     GKFLK"
     CDS             3819..4850
                     /codon_start=1
                     /db_xref="GOA:Q8KMW0"
                     /db_xref="InterPro:IPR001296"
                     /db_xref="UniProtKB/TrEMBL:Q8KMW0"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1045:glycosyl transferase group 1 (Score: 73.8;
                     E-value: 2.6e-22)"
                     /gene_kind="biosynthetic-additional"
                     /product="putative glycosyltransferase"
                     /protein_id="CAD19794.1"
                     /transl_table=11
                     /translation="MIIYIAAYNGSGGQGGVERVVAQQCNILKNLGVKVIILDKTYFKI
                     SNKIRNKKIQVALYPILVSLYLTLQKLRGVTFKVIAHGYCSPFYRNDILIAHGNMKCYF
                     QTVMNKKPNRLPGSGLLSFYERWAGAFSKNIWAVSNKVKSEWNELYNINSHKIKVVRNF
                     INLAQFDYTDVNEAEYVTFVGRLEKGKGIDDLYYICKNLPDTSFHLVSSIPAPQNFASL
                     NNVLTSIAVPYAKMPEIFKKSRVLILPSYYEGYELVTIEALCCGCPVIGYNVGAIRELY
                     AESFPGVFIANNKEDLAQVAYKLISLDNEKYYHLRQTIYSKRELFSEERYAEILTAAFN
                     EKK"
     CDS             4837..5982
                     /codon_start=1
                     /db_xref="GOA:Q8KMV9"
                     /db_xref="UniProtKB/TrEMBL:Q8KMV9"
                     /product="putative glycosyltransferase"
                     /protein_id="CAD19795.1"
                     /transl_table=11
                     /translation="MKKNKKLCLISINSYNELTGGGVYLRTLVSFLQKQNVNLTLIDKK
                     SSGKLFEDNTFQHISFIKGKRQDIISRLFFIPSFYVPYIFSIIKILRKQDILAFHNSRL
                     GLLCLLFRILMPHKKIILFTDNFEYDLIRQKDKNITTFIEKLIVYLNEFIGLKNSDLVG
                     YITRQDKNAMDKFYGIKKSRNLILPVIFSREKPTDVLSAHFINEYNRLNNDNRKKVVFT
                     ASFDFFPNIDAANYVLNAAKSNNDYCYILAGRKSTTLNLPDLDNLFFFDNLSNSEMSYL
                     LSACDVFYSPIVLGSGMKTKIAEALSYGLYIYATEHSLIGYDEIINNKECVKKISHLDE
                     EFPKDFKMKSINKQLIMSYQQKYYSHYRFNGHELDIINFDD"
     CDS             5999..7006
                     /NRPS_PKS="Domain: NAD_binding_4 (4-181). E-value: 1.4e-11.
                     Score: 35.8. Matches aSDomain:
                     nrpspksdomains_CAD19796.1_NAD_binding_4.1"
                     /codon_start=1
                     /db_xref="GOA:Q8KMV8"
                     /db_xref="HSSP:P04397"
                     /db_xref="InterPro:IPR001509"
                     /db_xref="InterPro:IPR005886"
                     /db_xref="InterPro:IPR008089"
                     /db_xref="UniProtKB/TrEMBL:Q8KMV8"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010:NAD-dependent epimerase/dehydratase (Score:
                     231.2; E-value: 3.1e-70)"
                     /gene_kind="biosynthetic-additional"
                     /product="putative epimerase"
                     /protein_id="CAD19796.1"
                     /transl_table=11
                     /translation="MNILVTGGAGYIGSHTAIELLNAGHEIIVLDNFSNASYKCIEKIK
                     EITRRDFITITGDAGCRKTLSAIFEKHAIDIVIHFAGFKSVSESKSEPLKYYQNNVGVT
                     ITLLQVMEEYRIKKFIFSSSATVYGEPEIIPIPETAKIGGTTNPYGTSKYFVEKILEDV
                     SSTGKLDIICLRYFNPVGAHSSGKIGEAPSGIPNNLVPYLLDVASGKRDKLFIYGNDYP
                     TNDGTGVRDFIHVVDLAKGHLAAMNYLSINSGYNIFNLGTGKGYSVLELITTFEKLTNI
                     KVNKSFIERRAGDVASCWADADKANSLLDWQAEQTLEQMLLDSWRWKKNYPDGF"
     CDS_motif       6008..6088
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CAD19796.1_0001"
                     /evalue="3.60E-04"
                     /label="PKSI-KR_m1"
                     /locus_tag="CAD19796.1"
                     /protein_end="30"
                     /protein_start="3"
                     /score="13.2"
                     /tool="antismash"
                     /translation="LVTGGAGYIGSHTAIELLNAGHEIIVL"
     aSDomain        6011..6541
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CAD19796.1_NAD_binding_4.1"
                     /evalue="1.40E-11"
                     /label="CAD19796.1_NAD_binding_4.1"
                     /locus_tag="CAD19796.1"
                     /protein_end="181"
                     /protein_start="4"
                     /score="35.8"
                     /tool="antismash"
                     /translation="VTGGAGYIGSHTAIELLNAGHEIIVLDNFSNASYKCIEKIKEITR
                     RDFITITGDAGCRKTLSAIFEKHAIDIVIHFAGFKSVSESKSEPLKYYQNNVGVTITLL
                     QVMEEYRIKKFIFSSSATVYGEPEIIPIPETAKIGGTTNPYGTSKYFVEKILEDVSSTG
                     KLDIICLRYFNPVG"
     CDS             7027..8232
                     /codon_start=1
                     /db_xref="GOA:Q8KMV7"
                     /db_xref="InterPro:IPR001296"
                     /db_xref="UniProtKB/TrEMBL:Q8KMV7"
                     /product="putative glycosyltransferase"
                     /protein_id="CAD19797.1"
                     /transl_table=11
                     /translation="MNQSEQRKKILVLTPRFPYPVIGGDRLRVYMLCKELSKKYDLILL
                     SLCDQPLELEININDSVFKEIHRVYLPKYKSYYNVLKALVTQKPLQIAYYQSDTFKNKY
                     NKLIKQCDAVFCHLIRVADYVKDTDKFKILDMTDAISLNYSRVKKLASKKSLRAIIYSL
                     EQKRLESYERSVANLFDLTTFISSVDRDYLYPNPGSNIHIVNNGVDTSALRYIKREIKI
                     DKPVELIFIGNMYSLQNMDAAKHFAKNILPCLYDEFNIIFKVIGKISETNKNILNSFKN
                     TIALGTVDDINSSASTGHIGICLVRLGAGVQNKILEYMALGLPCITSSIGYEGINAKSG
                     SEIFVADTVEQYKNVLREIIYDYNRYTEVAENARSFVENNFSWESKVANLMNTLDEKLY
                     EQ"
     gene            8210..9661
                     /gene="manC"
     CDS             8210..9661
                     /codon_start=1
                     /db_xref="GOA:Q8KMV6"
                     /db_xref="InterPro:IPR001538"
                     /db_xref="InterPro:IPR005835"
                     /db_xref="InterPro:IPR006375"
                     /db_xref="InterPro:IPR011051"
                     /db_xref="UniProtKB/TrEMBL:Q8KMV6"
                     /gene="manC"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1064:glucose-1-phosphate
                     adenylyl/thymidylyltransferase (Score: 55.7; E-value:
                     6.7e-17)"
                     /gene_kind="biosynthetic-additional"
                     /product="putative mannose-1-phosphate guanosyltransferase"
                     /protein_id="CAD19798.1"
                     /transl_table=11
                     /translation="MRNYMNNNKIITPIIMAGGSGSRLWPLSRILYPKQFLSLIGSHTM
                     LQTTANRLDGLDCTNPYVICNEQYRFIVAEQLRKIDRLTSKNIILEPVGRNTAPAIALA
                     ALLMSKSDKSADDLMLVLAADHVIHDEEKFCNAVRSAIPYAADGKLVTFGIIPDKAETG
                     YGYIHRGQYINQEDSDAFIVSSFVEKPNHETATKYLASGEYYWNSGMFLFSANRYIEEL
                     KQFRPDILSACEKAIASANFDLDFVRLDESSFSKCPEESIDYAVMEKTKDAIVIPMDAG
                     WSDVGSWSSLWEINDKDSDGNVIVGDIFSHETKNSFIYAESGIVATVGVENLVVVQTKD
                     AVLVSERNKVQDVKKIVEQIKNSGRSEHYVHREVYRPWGKYDSIDTGERYQVKRITVNP
                     GEGLSLQMHHHRAEHWIIVSGTARVTIGSETKILSENESVYIPLGVIHCLENPGKIPLD
                     LIEVRSGSYLEEDDVIRFQDRYGRS"
     gene            9744..11114
                     /gene="manB"
     CDS             9744..11114
                     /codon_start=1
                     /db_xref="GOA:Q8KMV5"
                     /db_xref="HSSP:P26276"
                     /db_xref="InterPro:IPR005841"
                     /db_xref="InterPro:IPR005843"
                     /db_xref="InterPro:IPR005844"
                     /db_xref="InterPro:IPR005845"
                     /db_xref="InterPro:IPR005846"
                     /db_xref="UniProtKB/TrEMBL:Q8KMV5"
                     /gene="manB"
                     /product="putative phosphomannomutase"
                     /protein_id="CAD19799.1"
                     /transl_table=11
                     /translation="MSSLPCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLGG
                     DVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIDFATFHLGVDGGIEATASHNPMDY
                     NGMKLVREGARPISGDTGLRDIQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLFGYI
                     NVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIPNPLL
                     PECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEASLEKNPGA
                     KIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHYFRDFAY
                     CDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINRVEQHFSR
                     EALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEEKTKLILELLNK"
ORIGIN
        1 tgaaaatctg accgaatgta acggttgata agaaaattat aacggcagtg aagattcgtg
       61 gcgaaagtaa tttgttgcga attttcctgc cgttgtttta tataaacaat cagaataaca
      121 acgagttagc aataggattt tagtcaaagt tttccaggat tttccttgtt tccagagcgg
      181 attggtaaga caattagcgt ttgaattttt cgggtttagc gcgagtgggt aacgctcgct
      241 acatcgtagg catgcatgca gtgctctggt agctgtaaag ccaggggcgg tagcgtgctg
      301 aaattataaa gtcattctta tagaacatcg catttcaata atataattac acctaaatga
      361 ataggataca acgtgtgcac aattatttaa ggcttaaaga taaaataaaa aacgtatttt
      421 tagggttgta tatattgcag ttatttaatt atatcgcgcc attggtaatt atccctatcc
      481 tgataaaata tattgggttg ggggaatatg gggaattggt ctatattaca tctatttatc
      541 aaatagtggc tttgattatt gattttggct ttacttacac aggacctgtg gttgctgcga
      601 gacatagatg tgagacccaa aatttacagc gctattactc aatagttgtt cttttaaaat
      661 cattgctttt tataattgca ttaacatgtg tatttttatt gtgcagatta aatatagtcc
      721 acttgtcatt ttttgggttt ttgtcaattt ttctatgcac tattggtaat atattatcgc
      781 ccaattggtt tttgcagggg attggtgatt ttaaaaaact ttcatactca caagtaatag
      841 tgagaataac attgtttatc atacttcttg tttatgtctg tagtggcgga gataatgttt
      901 ttatcctaag ttttttgcaa aatgcaacat tactcatatg ctgtatatac ttatggccaa
      961 atattcatat tagccatgtt gttcatctta aacctaatga atgcattgtg gaatttaaga
     1021 aggcaggaaa tgtttttatt ggcgtaatag gtacgattgg ttacaatggt ctaattcctg
     1081 tgttaattgg aaacctttgc ggtaatacga gtcttggtgt tttttcaatc gttcaaaaaa
     1141 tgacaacagc atgtcaaagt ctaattaatc caatatcaca gtatatgtta tctcaagttt
     1201 cagaaattaa acctcaagat aaactgtttt attatagaat taaaaaaagt ttttttgtgc
     1261 atttaacaat tagcataatt gcatgtttat gttatatggg attagggcaa tatgtggcga
     1321 cttttatagg taaagttgac gtttcatttg ttattatttt atttgcgtca ataattacca
     1381 ttttttcatc tttaaataat gtccttggta tacagtttct tataccgaca gataatgtaa
     1441 aaatactacg aagtataaat gttatggcgg gaattattgt tgttagtttg tcctggctgt
     1501 taatatcacg ctttgacatt ctgggggggg ttttattaaa cctaattggt gagtttcttg
     1561 tattcagtat gctagctttt attgcccatc gaaagtgggg agcgagagta taatgaaagt
     1621 gaaggcggtt cctgctatta cattctattt aagtttaatg ctgacaattt tagtgttact
     1681 gtttggtaat gaaccaaata aatcacaata tatccttgtt atagcaacga taacagtttt
     1741 ttatatcgca tatatcacta ataaaataac ttctccggcc agccttctcg ttatatcatc
     1801 ttttgtgttt ttaggttgtc gccctttatt atctttgttt gcaaactatg attataggat
     1861 tgccgattgg tttattgaag gatatatgga tgacgatgtg attttggcta actatgctat
     1921 aacactaatg tattatggtt atacattggg actaattcta tgcaaaaata ctgaaaaatt
     1981 ttatccgcat ggtccttatc ctgaaaaaca attgctaaaa ataaagtttc ttttgacttt
     2041 attttttctg ggttcgatag gtatggttgt aaaagggata ttctttttta actttataga
     2101 atctaatagt tatgttgata tttatcaatc aaatataaca acgccaatag gttatgattt
     2161 tctatcttat ttattttatt gttctttttt ccttatatgt gcgtttcata tacagttcag
     2221 aacaaataaa aaatttcttt ttattgcgat atgcattgct gcatttagca ccttgaaggg
     2281 tagtcgtagt gaagctataa cgtttctttt aacggttaca tgtatatatt ttaatgaagt
     2341 aaagacaaga aacttacgtc tgctgattac aatgattttt gtttttagcg tcatttttgt
     2401 gattagtgaa tttatctcaa tgtggcgcac tggagggagt ttttttcaat taatgcaggg
     2461 taataatcct gttataaact ttgtatacgg catgggagta tcatatcttt ccatttatca
     2521 atcagtaaaa ctacaactat tgtcaggggg atataatgtt acctatctat tcagccagtt
     2581 aataataact tgctcgtaaa tatttaatgt taaattgagc ttgccggaaa taagctatag
     2641 ccatttggcc tcatacacag caaacccaga actatataat cttgggttcg gacttggggg
     2701 gagttattta gcagaatcgt ttttagcatt tggtctgatt ggatgtttca ttataccctt
     2761 tttactttta cttaatttaa atgtattgga aaaatataca aaaaacaaac caattatata
     2821 ttttgtttat tatagtgtgt tgccacctat attattcaca ccaagagaga ctttgttcta
     2881 tttcttcccc tatcttgtca aaagtatatt tgttgctttt ttagttacat tatacatcca
     2941 gtataaaaag gattgaccaa aatgtcagaa aaaaatgtca gcataataat cccaagttat
     3001 aacagggctc atattcttaa ggaggtcata ccaagttatt ttcaggatga gactttagag
     3061 gttatagtta tcaatgatgg atcaacagat aatacaaata gtgtattagc tgaactgaag
     3121 gaaaaatatt ctcagttagt tattttagaa aatgaaacga ataaaaaaca gatgtattct
     3181 aaaaaccgag ggattgaaat agccaaaggg aaatatattt tttttggtga tgatgactct
     3241 tacctcttac ccggtgttat atctcggtta ttggctacaa aatatgagac aggcgctgat
     3301 gtaatcggcg caagaatact ttatatgaat aataacgaga aaacaattga agattgcata
     3361 aatcgacata aaaaagaggg gcgttttgtt agtgatctaa atagattgga ttttagttat
     3421 acatgtgatt tggaccatcc gattgaatgt ttttatgcac agccttttgt tctagctgaa
     3481 agggaactaa tatcgaaata tcgatttgat atatcttata cgggaaactg ctatcgtgag
     3541 gaaactgatt tcatgctatc tctatttatt aaaaataaaa aatttatata tgattcaaag
     3601 gctttgttaa taaatttacc tccaagaaaa gcgacgggag gggcaagaac agctaatcga
     3661 ttaaaatatc attacgaaag ttgcataaat aattatagat ttttaaaaaa atataatgat
     3721 aatttgaatc ttctttcagg acaaaagcat gctatatttt accgacagtg tcaattcgtt
     3781 ctgctaaaaa tgaagtcgtt tatcgggaag tttttaaaat gattatatat atcgccgcgt
     3841 ataatggttc aggagggcaa ggtggggtgg aaagggttgt tgcccaacaa tgtaacattc
     3901 ttaaaaattt gggggttaaa gtcattatac ttgataaaac atacttcaaa atttctaaca
     3961 aaattcgtaa caaaaaaata caagtagcac tttatccaat attagtttct ctttatttaa
     4021 ccttacaaaa attacgtggc gtgacgttta aagttattgc acatggctat tgttctcctt
     4081 tttataggaa tgacatctta atagctcatg gcaatatgaa atgttatttt caaacagtca
     4141 tgaataaaaa acctaatcgg ttgcctggca gtggtctttt atctttctat gagcgttggg
     4201 ctggagcatt ttcaaaaaat atctgggctg tttcaaataa ggttaaaagt gaatggaatg
     4261 agctttacaa tattaattca cataaaatca aagttgttcg aaattttata aatcttgcac
     4321 aatttgatta cactgatgtt aatgaagcag aatatgtgac atttgtcggg cgattggaaa
     4381 aaggaaaagg aatagatgat ctgtattaca tatgtaaaaa tctgccagat acttccttcc
     4441 atttagtttc aagtattccc gccccacaaa attttgcttc gctaaataat gttctgacca
     4501 gcattgctgt cccctatgcg aaaatgccag aaatatttaa gaaatccaga gtacttattt
     4561 taccgtccta ttatgaagga tatgagctgg ttactattga agcgctatgc tgtggttgcc
     4621 ctgtgatagg ctataatgtt ggtgcaatta gagagttgta tgcagaaagt tttcctggcg
     4681 tatttattgc caataataaa gaagatttag cacaagtagc ctacaaatta attagtcttg
     4741 ataatgaaaa atattatcat ttgagacaaa ctatttatag caagcgtgag cttttttctg
     4801 aagagagata tgcggaaatt ttaacggcgg catttaatga aaaaaaataa gaaactctgt
     4861 ctcatttcaa ttaactcata taatgaactt accggaggag gagtatattt acgtacgctt
     4921 gttagttttc tacaaaaaca gaatgttaat ttaacactta ttgataaaaa atcctcaggt
     4981 aaactattcg aagacaatac ttttcaacat atatcattta ttaaaggtaa acgtcaggat
     5041 ataatatcca ggcttttttt tataccatca ttttatgtcc cttatatttt ctcaataatt
     5101 aaaattttac ggaagcaaga tattcttgct tttcacaact ctcggcttgg attgttatgt
     5161 ctgcttttta gaatactcat gccccacaaa aagatcatat tgtttacgga taacttcgaa
     5221 tatgacttaa taagacaaaa agataaaaac ataactactt ttattgaaaa attaattgtt
     5281 tatctcaatg aatttatcgg gcttaagaat tcagatttag ttggctatat tacccggcaa
     5341 gataaaaatg caatggataa attttatggg attaaaaaaa gcagaaattt aattctccct
     5401 gtgatattta gtagagaaaa accaactgat gtattgtcag ctcactttat taatgagtat
     5461 aatcgattga ataatgataa taggaaaaaa gtagtattta ctgcatcttt tgattttttt
     5521 ccaaatatag atgctgccaa ctatgtttta aatgcagcaa agtctaataa tgattattgc
     5581 tatattttgg caggtaggaa aagtactact ttgaatcttc ctgatttgga taatttattt
     5641 tttttcgata atctatctaa tagtgaaatg tcatatttat tatctgcttg tgatgttttt
     5701 tattctccta tagttttagg aagtggaatg aaaacaaaaa ttgcagaagc actatcatat
     5761 ggattatata tttatgcgac agagcattcc ttaatcggct atgatgaaat tataaacaat
     5821 aaggagtgtg ttaaaaaaat ctcacatttg gatgaggaat ttcctaaaga tttcaagatg
     5881 aaaagtatca ataaacagct aataatgtct tatcagcaaa aatattattc acattatcgg
     5941 tttaatggcc atgaacttga tataataaat tttgacgatt agttagtgga gatataatat
     6001 gaacatatta gtaactggtg gtgctggata tatcggatct catacggcta ttgaattact
     6061 gaatgcaggt catgagatta tcgttctgga caatttcagt aatgcttcat acaagtgtat
     6121 cgaaaaaata aaagaaatta ctcgacgtga ttttataaca attactggag atgctgggtg
     6181 taggaagaca ctctccgcta ttttcgagaa acacgccata gatatagtta ttcattttgc
     6241 tggctttaaa tctgtttcag agtctaaaag tgaaccctta aagtattacc agaataatgt
     6301 tggagtgacc attactttat tacaggtaat ggaagagtac agaattaaaa aatttatctt
     6361 tagctcatct gcgacagtct atggtgaacc agagataatt ccaattccag aaacagctaa
     6421 aattggagga actacgaatc catatggcac atcgaagtat tttgttgaaa aaattctaga
     6481 ggatgttagt tccacgggaa aactggatat aatttgcttg agatatttta atcctgtcgg
     6541 tgctcattct agtggtaaaa taggtgaggc tccatctggt atccctaata atcttgttcc
     6601 ttatttattg gatgttgcga gtggtaaacg tgataaatta tttatttatg gcaatgatta
     6661 ccctactaat gatggaacag gtgtaaggga ttttattcat gttgttgact tagcgaaagg
     6721 tcatttggct gcaatgaatt atttaagtat caattcggga tataatatct ttaatcttgg
     6781 tacaggaaaa ggttattcgg tgcttgaatt aatcactaca tttgaaaaat taacaaacat
     6841 taaggtcaat aaatctttta tagagagaag ggcaggggat gttgcgtctt gttgggctga
     6901 tgcagataaa gctaattctt tattggactg gcaagccgaa caaactctag aacagatgtt
     6961 attggactcg tggcgttgga aaaaaaatta tccagacgga ttctgaatat aaaaggtttc
     7021 agttttatga atcaatcaga gcagagaaaa aaaatactgg ttcttacacc tcgctttccc
     7081 taccctgtca ttggagggga tagattaaga gtctatatgt tatgtaaaga actttccaaa
     7141 aaatatgatc ttattcttct gagcttatgt gatcaaccac tagaacttga aataaatata
     7201 aatgactcgg tcttcaaaga aattcatcgt gtctatctac caaaatataa atcatattat
     7261 aatgtattaa aagctttggt tacgcaaaaa ccgttgcaaa ttgcttatta tcaatcggac
     7321 acatttaaga ataaatacaa taaattaatt aaacaatgcg atgcagtatt ttgtcatctg
     7381 ataagagttg ctgattatgt taaggataca gacaagttca aaattcttga tatgacagat
     7441 gcaatatctt tgaattacag tcgcgttaaa aaattagcaa gtaaaaaaag tttgcgtgca
     7501 attatttatt ctctggaaca aaaaagatta gaatcatatg aacgttctgt ggcgaatctt
     7561 tttgatttga ccacttttat ttcatccgta gaccgtgact atctctaccc taatccgggc
     7621 agtaatatcc atatagtcaa taatggggtt gatacatcag ccttgagata tataaaaaga
     7681 gaaataaaaa tcgataagcc tgtggaactt atatttatcg gaaatatgta ttctttacaa
     7741 aatatggatg ctgcaaaaca ttttgctaag aatattttac cttgcttgta tgatgagttt
     7801 aatattattt ttaaagtgat tggtaagatc tcagaaacta ataaaaatat attaaattca
     7861 tttaaaaata caattgcttt aggtactgtt gatgatatca attcttccgc ttctacaggg
     7921 catataggta tatgtcttgt tcgtcttgga gcaggcgtac aaaataaaat tcttgaatac
     7981 atggctttag gtttaccatg tattacatct agcattggtt atgaaggtat taatgcaaaa
     8041 tcaggtagcg aaatttttgt tgcagataca gtagagcaat ataaaaacgt actaagagaa
     8101 ataatttacg attataatcg ttatactgaa gtggctgaaa atgcccgtag ttttgtagaa
     8161 aataattttt cttgggaatc aaaagttgcc aatttaatga atacattaga tgagaaatta
     8221 tatgaacaat aataaaatta ttacacctat cattatggct ggtggttcag gcagtcggtt
     8281 gtggccacta tcaagaattc tctatccgaa acaatttctt agcctaatcg gtagtcatac
     8341 catgcttcaa acaacggcta atcgtctgga tggtttggat tgtaccaacc cttatgtcat
     8401 ttgtaatgaa caataccgct ttatagttgc tgaacagctt agaaaaatcg atagattgac
     8461 ttcaaagaat atcatccttg agcctgttgg gcgtaacact gcccctgcaa ttgcattagc
     8521 ggcgttgctg atgtctaagt ctgataaaag tgcagatgat cttatgctcg tactggctgc
     8581 agatcacgtt atacacgatg aagaaaaatt ttgtaacgct gttagatcgg caattccata
     8641 cgctgctgat gggaaattgg taacatttgg tataattcca gacaaagcag aaactggtta
     8701 tggttatata catcgaggac aatatattaa tcaggaagat tcggatgcat ttatagtgtc
     8761 atcatttgtt gaaaagccaa atcatgagac agccactaaa tatcttgctt ccggtgagta
     8821 ttattggaat agcggtatgt ttttgtttag tgcaaatcgt tatatagagg aacttaaaca
     8881 atttcggcct gatattttat ccgcttgtga aaaagcaatt gcttcagcga actttgacct
     8941 tgattttgtg cgtttagatg aaagttcttt ctctaagtgt cctgaagagt caattgatta
     9001 cgctgtaatg gaaaaaacaa aagacgcaat tgttattcca atggatgctg gctggagtga
     9061 tgtcggttca tggtcttctc tttgggaaat taatgataaa gactcagacg gcaacgtaat
     9121 agttggggat attttctctc atgaaacaaa gaattctttc atatatgccg aatcgggaat
     9181 tgttgctaca gttggagtgg aaaatttagt tgttgtccaa acaaaggatg ctgttcttgt
     9241 ctcagagaga aataaagttc aggatgtaaa gaaaatagtc gaacaaatta aaaattcagg
     9301 tcgtagcgag cattatgttc atcgcgaagt atatcgtcct tggggtaaat atgattccat
     9361 tgacacaggg gagcgttatc aggtcaaacg tataacagta aatcctggtg aaggactttc
     9421 tttacaaatg caccatcata gggcagaaca ttggatcata gtttctggaa ctgcaagggt
     9481 gactataggt tctgaaacta agattcttag cgaaaatgaa tctgtttaca tacctcttgg
     9541 tgtaatacac tgcttggaaa atccagggaa aattcctctt gatttaattg aagttcgttc
     9601 tggatcttat ttagaagaag acgatgttat ccgttttcag gaccgatatg gtcgtagcta
     9661 aatttttgat aatgtaacgt tagtagaaga gcgctaatat ttttagttaa tctgtaataa
     9721 gtattatttg tttaaggtat atcatgtcga gtttaccctg ctttaaagcc tatgatattc
     9781 gcgggaaatt aggcgaagaa ctgaatgaag atattgcctg gcgcattggt cgcgcttatg
     9841 gcgaatttct caaaccgaaa accattgtgt taggcggtga cgtccgactc accagcgaaa
     9901 ccttaaaact ggcgctggcg aaggggttac aggatgcggg cgtcgatgtg ctggatattg
     9961 gcatgtccgg caccgaagaa atcgatttcg ccacgttcca tctcggcgtg gatggcggca
    10021 tcgaagccac cgccagccat aacccgatgg attacaacgg catgaaactg gtgcgcgaag
    10081 gggctcgccc gatcagcggt gataccggac tgcgcgacat ccagcgtctg gcagaagcca
    10141 acgactttcc tcccgttgat gaaaccaaac gcggtcgcta tcagcaaatc aatctgcgtg
    10201 acgcttacgt tgatcacctg ttcggttata tcaacgtcaa aaacctcacg ccgctcaagc
    10261 tggtgattaa ctccgggaac ggcgcggcgg gtccggtggt ggacgccatt gaagcccgct
    10321 ttaaagccct cggcgcaccc gtggaattaa tcaaagtgca caacacgccg gacggcaatt
    10381 tccccaacgg tattcctaac ccgctactgc cggaatgtcg cgacgacacc cgcaatgcgg
    10441 tcatcaaaca cggcgcggat atgggcattg cctttgatgg cgattttgac cgctgtttcc
    10501 tgtttgacga aaaagggcag tttattgagg gctactacat tgtcggcctg ctggcagaag
    10561 cgtccctcga aaaaaatccc ggcgcgaaga tcatccacga tccacgtctc tcctggaaca
    10621 ccgttgatgt ggtgactgcc gcaggcggca ccccggtaat gtcgaaaacc ggacacgcct
    10681 ttattaaaga acgtatgcgc aaggaagacg ctatctacgg tggcgaaatg agcgcccacc
    10741 attacttccg tgatttcgct tactgcgaca gcggcatgat cccgtggctg ctggtcgccg
    10801 aactggtgtg cctgaaagga aaaacgctgg gcgaactggt gcgcgaccgg atggcagcgt
    10861 ttccggcaag cggtgagatc aacagcaaac tggcgcaacc cgttgaggcg attaaccgcg
    10921 tggaacagca ttttagccgt gaggcgctgg cggtggatcg taccgatggc atcagcatga
    10981 cctttgccga ctggcgcttt aacctgcgct cctccaacac cgaaccggtg gtgcggttga
    11041 atgtggaatc gcgcggcgat gtaccgctga tggaagaaaa gacaaaactt atccttgagt
    11101 tactgaacaa gtaattcagt aatttcatat aaatgggttt taaaaaacgg aaaagatgag
    11161 atatccggtg tggtatatcc aaggtaatgc tattcagtat ctctatgggt gagttaacat
    11221 ctataccaca tttaagccgc acacttcgcg gtgaccaccc ctgacaggag taaacaatgt
    11281 caaagcaaca gatcggcgtc gtcggtatgg ca
//
