LOCUS       BGC0000786             12215 bp    DNA     linear   BCT 03-JUN-2003
DEFINITION  Edwardsiella ictaluri isolate 93-146 O antigen biosynthesis gene
            cluster, partial sequence; and IS1-like insertion sequence, complete
            sequence.
ACCESSION   BGC0000786
VERSION     BGC0000786
KEYWORDS    .
SOURCE      Edwardsiella ictaluri 93-146
  ORGANISM  Edwardsiella ictaluri 93-146
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Hafniaceae; Edwardsiella.
COMMENT     On Mar 5, 2002 this sequence version replaced AY057452.1.
            ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2024-10-17 14:12:21
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 12215
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     gene            <1..1204
                     /gene="dcuC"
     source          1..12215
                     /db_xref="taxon:634503"
                     /mol_type="genomic DNA"
                     /organism="Edwardsiella ictaluri 93-146"
                     /strain="93-146"
     subregion       1..12215
                     /aStool="mibig"
                     /contig_edge="True"
                     /label="saccharide"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..12215
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     CDS             <1..1204
                     /codon_start=2
                     /gene="dcuC"
                     /product="C4-dicarboxylate anaerobic carrier"
                     /protein_id="AAL25626.1"
                     /transl_table=11
                     /translation="MTGWRATDIVEYVKVLLMSRGGDLGMMIMMLCGFAAYMTHIGAND
                     MVVKLASRPLRYINSPYILMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISR
                     GAAAAICASPAAIILAPTSGDVVLAAKASEMPLIDFAFKTTLPISIIAIVCMAIAHYFW
                     QRYLDNKEQAQHEILDVNEITTTAPSFYAILPFTPIIGVLVFDGKWGPHLHIISVLVIC
                     MLLASVTEFLRSFDAKKVYSGLEVAYRGMADAFASVVMLLVAAGVFAQGLSTVGFISSL
                     IELAQSFGTGGIIMMLVLVVITMLAAMTTGSGNAPFYAFVELIPKLSGQMGINPAYLTI
                     PMLQASNLGRTLSPVSGVVVAVSGMAKISPFEVVKRTSVPVLVGLVVVIVATEFMVPLH
                     R"
     gene            2079..2312
                     /gene="insA"
                     /note="similar to InsA protein"
     mobile_element  2079..2746
                     /mobile_element_type="insertion sequence:IS1-like"
     gene            2354..2746
                     /gene="insB"
                     /note="similar to InsB protein"
     gene            2984..4231
                     /gene="wzx"
     CDS             2984..4231
                     /codon_start=1
                     /gene="wzx"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt"
                     /gene_kind="biosynthetic-additional"
                     /product="O antigen transporter"
                     /protein_id="AAL25627.1"
                     /sec_met_domain="Polysacc_synt (E-value: 2.9e-41, bitscore:
                     131.1, seeds: 31, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSSLLKNSISLLAMQGINYILPLVTLPYLTRVLHVYPFGIYSATM
                     SVGAYVILFINFGFDLSATRKIAKNKGDNYAISAIFFNVILAKFLLFFLSLVIVFLLLY
                     TIDSFYPIRSLFFLLIPQFIGCVFFPAWLYQGVERLYLISAITSATKLLIIPLFFLFVR
                     DAGDIDKAIIISGFPLFFSGIIGFYLAYKLNIINFSDVRVRVNDAILLIKESTSVFIGA
                     VAISIYTACTPIILSIVSNYTEVGYFSAVDKLRSAILGMFIVLGQVFYPRATVLFESNF
                     SKYKLFIRRIIFSQIIFGGVGTILFYFFMPIIAPFILGRGFHGLTELIEIMAPMILLIP
                     LSVIFSNCILLPMGKSKLFYIAPIITAIIHLPYSIYLSEKYGAFGGGVSILITEVLSLM
                     LLVFFSYKYANLKSIF"
     gene            4791..5954
                     /gene="wbeiA"
     CDS             4791..5954
                     /codon_start=1
                     /gene="wbeiA"
                     /inference="non-experimental evidence, no additional
                     details recorded"
                     /product="WbeiA"
                     /protein_id="AAL25628.1"
                     /transl_table=11
                     /translation="MFPTKEFKYFDFFCVIEGLFKVLLSKLSVNRSVNLLFFYLFFSVF
                     VLIFIFKPPFHFFLLNALFIHVYSLVALIFSFFYRVKRKYIIIFFIFLFPLSYLTIMDR
                     VTAAYPLVVALFSLLIISDKFQFHNYSCRNLTHFILISTVLFSFLFLYESHIDRYSLVN
                     GDPNYTSLILLTVLLISFVFSVSNKLKVLVLLLMLFVVYLTKSRTAILALAAFFLCCKI
                     KRKLLLFYFILFVSIASQYFFSYIFPSILSDLDFGHSRFFDLNDASNMERITIYKEAVD
                     FILSHFTHFIINGVTNYLEINKLATNIPHNWFIQFILGFGFLFSIYYLAVVVFVAKIVS
                     GKSYYLLAFMCFFFVYASFLSYYSLATPLLFLVLSLFVCHSIERVAR"
     gene            6029..6814
                     /gene="wbeiB"
     CDS             6029..6814
                     /codon_start=1
                     /gene="wbeiB"
                     /note="includes previous wbeiC gene, whose presence was
                     annotated because of an error in the sequence"
                     /product="putative glycosyltransferase"
                     /protein_id="AAL25629.2"
                     /transl_table=11
                     /translation="MKTFIISLKDEVARRNSISDRLHHHDFEFFDAVDLRVASDDVLSA
                     VKCKNINYKHPAIREHMTKGEIGCALSHMQLYKKIVDDNLAFARVIEDDAVILNHDEKI
                     LNDFILALEMKNIDWDIMLLGYSKLKACDSFGFYLKEPIKNIVKSGAYSLGIPFRNWTC
                     GTVGYLVSQSGAKKMINNAAFGKVCTVADDWLFFEKNYNLKILHIRPLIVLEDFESYES
                     SIETDRKTYVNHESHISFFLRVMRGVVRKVYLSISWKSL"
     gene            6811..7911
                     /gene="wbeiM"
     CDS             6811..7911
                     /codon_start=1
                     /gene="wbeiM"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1045:glycosyl transferase group 1 (Score: 153.7;
                     E-value: 1.4e-46)"
                     /gene_kind="biosynthetic-additional"
                     /product="putative glycosyltransferase"
                     /protein_id="AAL25631.1"
                     /sec_met_domain="Glycos_transf_1 (E-value: 1.2e-41,
                     bitscore: 131.7, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKILYVITGLGLGGAEKQLSLLADNFTARGEQVSIVYLTGEVLVK
                     PKNKNIKIYNLGIDKSFSSLIKGIWKLKSIISDVRPDVIHSHMYHANILARISCCLSLF
                     SSRLVCSAHNKNEGGRVRMIIYRMTDFLCAKTTNVSQEALDEFITKKAFRKRKSSLVYN
                     GIDLSIFKKKSTNIQNIKNKLGINFDEKVIFCAGRLTEAKDYPNLILAISKMHQKKCKI
                     IIAGDGPMRSDIERLIDRCHLSHRILLIGIIDNISDYYNLSDLFVLPSRWEGFGLVVAE
                     AMACECPVIATDAGGVAEVLSNADWLVPIADSSKLAEKIDEFFLLDSSEVKDIKAKNKD
                     HCENQFSIGAIINNWYAIYNGIRSKL"
     gene            7913..8950
                     /gene="wbeiH"
     CDS             7913..8950
                     /codon_start=1
                     /gene="wbeiH"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1045:glycosyl transferase group 1 (Score: 95.4;
                     E-value: 6.9e-29)"
                     /gene_kind="biosynthetic-additional"
                     /product="putative glycosyltransferase"
                     /protein_id="AAL25632.1"
                     /sec_met_domain="Glycos_transf_1 (E-value: 7.5e-22,
                     bitscore: 67.2, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKIFFVITKSEIGGAQKWIVELSNILKEQYEIFIITSGDGWLTS
                     KFDSSHVILYPGLLNLKSIFCFFKLANIFRKYKADIVISSSANAGVYARLSKFLYSHKS
                     IYVSHGWSCLYNGGRLKKIQCLVERFLSLISDKILCVSENDMNNAIKFIGIKEKKLFVI
                     RNGVSPLKKKNVINVRKKVLFVGRMIHPKRPDLFVAVADSFPDVDFYLVGDGPLKQRLE
                     TSCQHNNVYFLGEINGFDSFQCYDIFVLCSDSEGLPMSALEAASSGVPILLSDVGGCSE
                     LITLMNNGCSNGELFSNDISSLRCKLGAVLLEYDKYFSSAQVVSGCFDINNLAEKYIEL
                     IEGDK"
     gene            8947..9918
                     /gene="wbeiT"
     CDS             8947..9918
                     /NRPS_PKS="Domain: NAD_binding_4 (49-174). E-value:
                     7.7e-15. Score: 46.5. Matches aSDomain:
                     nrpspksdomains_wbeiT_NAD_binding_4.1"
                     /codon_start=1
                     /gene="wbeiT"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) NAD_binding_4"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010:NAD-dependent epimerase/dehydratase (Score:
                     135.4; E-value: 4.8e-41)"
                     /gene_kind="biosynthetic-additional"
                     /product="UDP-glucose 4-epimerase"
                     /protein_id="AAL25633.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 5.6e-21, bitscore:
                     64.2, seeds: 37, tool: rule-based-clusters)"
                     /sec_met_domain="Polysacc_synt_2 (E-value: 1.2e-09,
                     bitscore: 27.1, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="NAD_binding_4 (E-value: 1.4e-15, bitscore:
                     46.5, seeds: 102, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLAIIGGSGFIGTRLATQLKNNNVEFTIVDKRNSETFPECWQYG
                     DVTKPESLEEPLKGADVIINLAAEHKDNVHPISLYYDVNVQGAKNVCDVAEKLNIQHIV
                     FTSSVAVYGFVEKETGEDGKYNPFNDYGKSKLEAEFAYDAWQAKVPERTLVTVRPTVVF
                     GENNRGNVYNLFRQIASGKFLMIGPGLNQKSMAYVENMAAFLRFSTTMGAGRHVFNYID
                     KPDFSMNDLTGVICTALHRHKNNIRIPYAVGLLGGYCFDILAKVTGREFPVSSIRVKKF
                     CARTQFKSNHIAQTGFKAPVTLEQGIANTVRYEFLEKHDDKS"
     aSDomain        9094..9468
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_wbeiT_NAD_binding_4.1"
                     /evalue="7.70E-15"
                     /label="wbeiT_NAD_binding_4.1"
                     /locus_tag="wbeiT"
                     /protein_end="174"
                     /protein_start="49"
                     /score="46.5"
                     /tool="antismash"
                     /translation="PESLEEPLKGADVIINLAAEHKDNVHPISLYYDVNVQGAKNVCDV
                     AEKLNIQHIVFTSSVAVYGFVEKETGEDGKYNPFNDYGKSKLEAEFAYDAWQAKVPERT
                     LVTVRPTVVFGENNRGNVYNL"
     gene            9905..10807
                     /gene="galF"
     CDS             9905..10807
                     /codon_start=1
                     /gene="galF"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1064:glucose-1-phosphate
                     adenylyl/thymidylyltransferase (Score: 177.3; E-value:
                     6.3e-54)"
                     /gene_kind="biosynthetic-additional"
                     /product="UTP-glucose-1-phosphate uridylyltransferase"
                     /protein_id="AAL25634.1"
                     /transl_table=11
                     /translation="MTSLKAVIPVAGLGMKMLPATKAIPKEMLPIVDKPLIQKVVHECL
                     SAGVKEIILVTHSSKNTIENHFDTSFELEALLEARVKRQLLDEVRSICPPGVTINSVRQ
                     HRQLGLGHAILCARPLVGEHPFIVALPDIWLDASTFDPETQNLAAMVRRFEESGRSQVL
                     VQPMPLEALPEYSVINCADAVLEPGQSACMTSIVEKPEDAEKYHSDLSAVGRYVLSAAI
                     WPILAATGFGAWERIQLSDAIATLLQHRPVEAYRQVGLSYDCGDKLGYAEAFVAGGLNH
                     SQQGAAFTAWLQDVQTRIE"
     gene            10823..11839
                     /gene="galE"
     CDS             10823..11839
                     /NRPS_PKS="Domain: NAD_binding_4 (4-176). E-value: 2.1e-12.
                     Score: 38.5. Matches aSDomain:
                     nrpspksdomains_galE_NAD_binding_4.1"
                     /codon_start=1
                     /gene="galE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010:NAD-dependent epimerase/dehydratase (Score:
                     261.5; E-value: 1.9e-79)"
                     /gene_kind="biosynthetic-additional"
                     /product="UDP-galactose 4-epimerase"
                     /protein_id="AAL25635.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 1.7e-12, bitscore:
                     36.4, seeds: 37, tool: rule-based-clusters)"
                     /sec_met_domain="Polysacc_synt_2 (E-value: 4.1e-19,
                     bitscore: 58.1, seeds: 77, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAILVTGGAGYIGSHTVLALLSRGDEVVVLDNFVNASVQALARVE
                     QICDRQPHLYRGDVRDRALLQQIFAQHVISDVIHFAGLKAVGESVEKPLEYYDNNVSGT
                     LVLLEEMRRAGVQGFIFSSSATVYGDPESVPLTESSRTGGTTNPYGTSKLMVEQILQDV
                     SRARPEMRITALRYFNPVGAHPSGLIGEDPNGIPNNLIPYVAQVAVGKLACLSIFGHDY
                     PTQDGTGVRDYIHVMDLATGHLAALDRRDEGASYKVYNLGTGVGYSVLDIVAAFERCSG
                     VTIPYRFAPRRAGDIAECWSNPALAAQELGWCAQYGLEAMMTDTWRWQQNNPNGYQD"
     CDS_motif       10829..10912
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_galE_0001"
                     /evalue="5.50E-04"
                     /label="PKSI-KR_m1"
                     /locus_tag="galE"
                     /protein_end="30"
                     /protein_start="2"
                     /score="12.6"
                     /tool="antismash"
                     /translation="ILVTGGAGYIGSHTVLALLSRGDEVVVL"
     aSDomain        10835..11350
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_galE_NAD_binding_4.1"
                     /evalue="2.10E-12"
                     /label="galE_NAD_binding_4.1"
                     /locus_tag="galE"
                     /protein_end="176"
                     /protein_start="4"
                     /score="38.5"
                     /tool="antismash"
                     /translation="VTGGAGYIGSHTVLALLSRGDEVVVLDNFVNASVQALARVEQICD
                     RQPHLYRGDVRDRALLQQIFAQHVISDVIHFAGLKAVGESVEKPLEYYDNNVSGTLVLL
                     EEMRRAGVQGFIFSSSATVYGDPESVPLTESSRTGGTTNPYGTSKLMVEQILQDVSRAR
                     PEMRITALR"
     gene            11863..>12215
                     /gene="ugd"
     CDS             11863..>12215
                     /codon_start=1
                     /gene="ugd"
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="AAL25636.1"
                     /transl_table=11
                     /translation="MKIAISGTGYVGLSNGILLAQHHEVVALDIVAEKVGMLNRRQSPI
                     VDKEIQEYLTNRSLNFRATLDKEEAYRGADFVIIATPTDYDPKSNYFNTASVEAVIRDV
                     LAINPAAVMIIKST"
ORIGIN
        1 gtcgaccggc tggcgcgcga ccgatatcgt tgagtatgta aaagttctgc tgatgagtcg
       61 cggcggcgac ctcggcatga tgatcatgat gctgtgcggt tttgccgcct atatgaccca
      121 tatcggtgcc aacgatatgg tggtcaagct ggcctcccgc ccgctgcgct atattaactc
      181 gccctatatt ttaatgattg cggcctactt tgtcgcctgc ctgatgtcgc tggccgtttc
      241 gtcggccacc gggctgggag tattgctgat ggcaaccctg ttcccggtga tggtcaacgt
      301 ggggatcagc cggggggcgg cggcggcgat ctgtgcctcg cctgcggcga tcatcctggc
      361 tccgacctcc ggcgatgtgg tgctggcagc caaggcgtcc gaaatgccgc tgatcgactt
      421 tgcctttaaa acaaccctgc ccatctccat catcgctatc gtctgtatgg cgattgccca
      481 ctacttctgg cagcgctacc tggacaacaa ggagcaggcg cagcacgaga tactggacgt
      541 caacgagatc accaccacgg caccctcgtt ttatgccatt ctgcccttta cgccgatcat
      601 cggcgtgctg gtgttcgacg gtaagtgggg gccacatctg cacatcatca gcgtcctggt
      661 tatctgtatg ctgctggcct cggtgactga gtttttgcgc agcttcgacg ctaaaaaggt
      721 ctatagcggg ctggaggtgg cctaccgcgg gatggcggac gcctttgcca gcgtggtgat
      781 gctgttggtg gccgccgggg tctttgccca ggggctgagc accgtcggct ttatcagcag
      841 cctgatcgag ctggcccagt cctttggcac cggtggcatc atcatgatgc tggtgctggt
      901 ggtgatcacc atgctggcgg cgatgaccac cggctccggc aacgcgccgt tctacgcctt
      961 tgtcgagttg atccccaagc tgtccggcca gatgggcatc aacccggcct acctgaccat
     1021 tccgatgctg caggcctcca atctgggccg caccctatcg ccggtttccg gtgtggtggt
     1081 ggctgtctcc gggatggcca agatctcccc ctttgaggtg gttaaacgca cctcggtgcc
     1141 ggtgctggtc ggcctggtgg tggtgatcgt cgccaccgag tttatggtac cgctgcaccg
     1201 ctgacaaatg caagcgatat acacggagag ggcaggccag aggcctgcct tttttggttt
     1261 gcggtggcaa gtgccagcca tgtggaaatg acatgtcgat aggctggttt tatgttactt
     1321 ttacgccttg gtggcggcag atgcagatgc agatattggt tatatacaca gggtgatata
     1381 caataatcgg aaagataaat tgtatgataa ttggccgtcg gagtttgaaa ataaaaattt
     1441 gagatgtttt accttttttc tattaagtat aaaaattagt gctgcttgtg tccactggtg
     1501 agagtgagcc gtggggaaat ctcacagccg tactgcgtta tgatgggctt gtttgcatgc
     1561 cgtgtcttgt ttttcaagga atatattgac agtgtggcat agatgtaaac acagtaaaaa
     1621 gttgctcagg gtagtcagtg cattggtagc tgtagagcca agggcggtag cgtaagaata
     1681 gcaatccttt tttaaccagt cgcctgtcac tatttgtttt tttagatgtt aacttctatt
     1741 tttatattta tgtaaaaaac ttctgataaa taaattaact aacaatttaa aatgactgtg
     1801 gtttaatatg caagatgatt gtaaggctgc ccattattta ttcaaaataa ataataatat
     1861 gtacagtggg taactccttc aattagatcc attccagtag gaatatatta ttgcacccag
     1921 tttagaccgg acagcgcgta acaaccggag cgtaaacgca gtacgtgagg attgtcagtt
     1981 cggcccagga ccaaaatggc aaatcaaata gcctaatggg atagagtctt agctgatatc
     2041 ggtgtataat tcccgctgtt gaggttgtct catggccagt gttaacgctc actgctccca
     2101 ttttcagtct gctcaggttt acaaccttta agggctgtga ccgggtccgc tgtcgtgatt
     2161 ggtaccctat attcctgctc tcttatacct atgaagcccg taagctcgga gttaaagagc
     2221 aaattactga aatggtattc aacggatccg atgtccggga tacaataaga acatccaaaa
     2281 tcggcattaa caccgtcatt attcgcactt aaaaaaacgc acgccgaggc gaataacatc
     2341 atccctgttg ctcatgaaga tgtggcgctt atctgcgaac tcgatgggca atagcgtttc
     2401 gtcggaatca aaacccgaca acactggttt tggtatgcat acaacaccac aacagatggc
     2461 gttctggtgt tataggtttg gaccacgtac ttatgatata ttccgtgagc tgctgattct
     2521 gcttacatcg tttaatctcg gcatgatagc cagcgatgac tgggtccgtt atgccagaga
     2581 attcccgaaa gagatgcctc tgatcggtaa aatatttact cagcggattg tgcataataa
     2641 tctgatactt tgcacccgca ttcagcggct gaatcgtaaa acaagctcag ctaagattaa
     2701 cgggaaaagc attggtacct tcatagaaaa atatatgttc tactaattgg atgcatcacc
     2761 gttggtgtgt tgattggtga agtggatcca atttttcatt aatatggaag agaattcata
     2821 tgttttttgt tctcaatagg tgggttgtta tttgttgatg ataaggccga ggtctatata
     2881 ttatttaatt taataaggaa gttgctttgt aaaataataa aaatattggt attgaggata
     2941 agttttgatg gctggaagca ttgttaaaat ataatggtat attatgagtt cacttctaaa
     3001 aaattccata tctttacttg caatgcaagg gattaactac atactaccac tagtaacgct
     3061 accttacttg actagagttc tgcatgtata tccatttggt atatatagtg caacgatgag
     3121 tgttggcgct tatgtaatat tgttcataaa ttttggtttt gatcttagtg cgacgaggaa
     3181 aatcgcaaaa aacaagggtg ataattatgc tattagcgcc atatttttta atgttatctt
     3241 ggctaagttt ttgttatttt ttctctcgct tgttatcgtg tttttattgc tttatacgat
     3301 tgattcattt tatcctatta ggtctttatt ctttttatta atacctcagt ttattggctg
     3361 tgtttttttt cctgcttggt tatatcaagg ggtggagcgg ttatatctaa tatcagcaat
     3421 aacatctgcc acaaagctat taattattcc tctgtttttt ttatttgtaa gagatgctgg
     3481 tgatattgat aaagctataa ttatctctgg tttcccgctg ttcttttctg gtatcattgg
     3541 tttttatcta gcatataagt taaatatcat caacttttca gatgtgaggg taagggttaa
     3601 tgatgctatt cttttgataa aagaatctac ttctgttttt attggggctg tggctataag
     3661 tatatatacg gcatgtactc caattatttt aagcattgtt agtaattata ctgaggtcgg
     3721 ctatttttca gcagtagata aattaagaag tgctatttta ggtatgttta ttgtgctagg
     3781 tcaggttttt tatccaagag ctactgtttt gtttgaaagt aatttttcaa aatataagct
     3841 atttataagg cgaattattt tttcgcagat tatttttggc ggggttggga cgatattatt
     3901 ttatttcttc atgccaatca ttgcgccatt tattttaggg cgtggttttc atgggttgac
     3961 agagctcatt gaaataatgg ccccaatgat attattaatt cctttgtctg taatattctc
     4021 taattgcatt ctactgccaa tggggaagag taaattgttt tatattgctc ccataataac
     4081 agctataata catttgccat actctattta tttgagtgaa aagtatggcg cttttggtgg
     4141 cggggtctcg atattaataa ctgaggtcct tagtttaatg ttgttagtgt ttttttctta
     4201 taaatacgca aatttgaaaa gcattttttg aagtgagagg attacataag gcacatgttg
     4261 ttttatggat tggagagtca attaggtgag ggctatatat aattataggc tttgttgaat
     4321 catgctgttt gcagtgttga ggggagggtt caagaagaca tatttctcta cacgctctaa
     4381 ccacatgatt tttcgtcact agggttggcc actatattga gataacagca taaaatagac
     4441 ttatacaaca acgcttaagc cacatgtaat aacaaacaaa tggtttgggg aatataacta
     4501 tgaacatccg tataaactcc tgaataacct gactctagag gagttttgga tgacgaatac
     4561 aagcgacgga agcctaaagg aattatgaaa ctaaagctgg cgtttttgca gtaggctatt
     4621 taataggaat gtatgcagct aaattaatga tagaaaatga taagccatca atctttagtg
     4681 attgtatact tcatgtgtat gatattaata gtaaaatatt acacatgtta tccatgtatg
     4741 attttagagg gttgttaaag gatgtgtaat tgtattaaag gtgatgggga atgtttccta
     4801 caaaagagtt taagtatttt gacttttttt gtgtaataga gggattgttc aaagtgttgc
     4861 tctcaaaatt gtccgttaat cgtagtgtga atcttttgtt tttttatttg tttttttctg
     4921 tctttgtgtt gatatttata ttcaagcctc cgtttcattt ttttttgcta aacgcattat
     4981 ttattcatgt atattcactg gttgctttga ttttctcttt tttctataga gtaaagcgaa
     5041 aatatatcat catctttttt atttttttat tcccattaag ttatttgact attatggaca
     5101 gagttactgc tgcatatccg cttgtggtgg ctcttttttc attattaatc atctctgata
     5161 agtttcagtt tcataattac tcctgtcgca atttaacgca ctttatttta atttctactg
     5221 ttcttttttc atttttattt ctgtatgaat cacacatcga tcgttattcg ttagttaatg
     5281 gcgatcctaa ctataccagt ttgatcttat taactgtgct acttatttct tttgtatttt
     5341 ctgtgagtaa taagcttaaa gtgttagttt tattattaat gctttttgtt gtttatttga
     5401 caaaatcaag aacggcaata ttggctttgg ctgcattttt tctatgttgt aagattaagc
     5461 ggaaattatt gttgttctat tttatattat ttgtgtctat agcctctcag tatttctttt
     5521 catacatatt cccttcaata ctgtctgatc tcgatttcgg ccatagccga ttttttgatt
     5581 taaatgatgc cagtaatatg gaaaggataa ctatttataa ggaggccgtg gattttatat
     5641 tatcccattt tactcacttt attattaatg gggtgactaa ttatttagaa ataaacaagt
     5701 tggctactaa tataccacat aattggttta tccagtttat tcttgggttt gggtttttat
     5761 tttctattta ttatttggcg gtggttgttt ttgttgcaaa aatagtttct ggtaagtctt
     5821 attatctatt ggcttttatg tgttttttct ttgtttatgc atcatttctc agttattatt
     5881 ctttagcaac tccattatta tttttggtgt tatctctatt cgtgtgccat agtattgaaa
     5941 gagtggcgag ataatgaatg ttgttttatg tcgattgcgt tataatattg agtgttttac
     6001 atccacatta taagaatcca ttgaacctat gaaaactttt attatatcct tgaaagatga
     6061 agtcgctcgt agaaatagca ttagcgatag gttacatcat catgacttcg agttttttga
     6121 tgcggttgat ttgagagtgg catcagatga tgtgctttca gcggtaaaat gtaaaaatat
     6181 taattataaa catccagcaa ttagagagca tatgacaaaa ggagagattg gatgtgcttt
     6241 aagtcatatg cagctatata aaaaaattgt tgatgataat ttagcatttg ctagagtaat
     6301 tgaagatgat gcggttattt taaatcatga tgaaaagatt cttaatgatt tcatattggc
     6361 tttggaaatg aagaatatag actgggatat catgttgctg ggatattcta agttaaaagc
     6421 atgtgattct tttggttttt atcttaagga gccaattaaa aatatcgtaa agagcggtgc
     6481 atactcttta ggaataccat ttaggaactg gacttgtgga acggtaggat atctggtttc
     6541 tcaaagtggt gcaaagaaaa tgattaataa tgctgccttt ggtaaggtat gcactgttgc
     6601 tgatgattgg cttttttttg aaaaaaatta taatttaaaa atacttcata taagacctct
     6661 aattgtattg gaggactttg agtcgtatga aagttcaata gagacagata gaaaaactta
     6721 tgttaatcat gaatcacata tctcattctt tttaagagtt atgagagggg ttgtaaggaa
     6781 agtatattta tctatctctt ggaaaagttt atgaaaatac tatatgttat tacagggtta
     6841 gggttgggtg gggcagagaa acaactttct ttattagcag ataattttac tgctagaggt
     6901 gagcaagttt caatcgttta tttaaccgga gaggtactgg ttaagccgaa aaacaaaaat
     6961 ataaaaatat ataatctagg tatagataaa agtttttctt cactaatcaa ggggatctgg
     7021 aaactaaaat caataatatc tgacgttagg cctgatgtga tccattcgca tatgtatcat
     7081 gctaatatct tagctcgtat ttcatgttgc ctttccctat tttcatctag gttagtgtgc
     7141 tcggctcata ataaaaacga gggggggcga gttaggatga ttatttatcg aatgactgat
     7201 tttctatgtg ctaaaacaac aaatgttagt caggaagctc tggatgagtt tataactaag
     7261 aaagcattta gaaaaagaaa gtctagttta gtctataatg gaatagatct ttctatcttt
     7321 aaaaagaaaa gcactaatat tcaaaatatt aaaaataaat taggaataaa ttttgatgag
     7381 aaggtgatat tttgtgcagg tagattaaca gaagcaaaag actatcctaa tttgatcctc
     7441 gcaatatcta agatgcacca gaaaaaatgt aagattatca ttgctggtga tggtcccatg
     7501 cgtagtgata tagagagact aattgatcgt tgccatctat cgcatcggat tttacttatt
     7561 gggataattg ataatatttc tgattactat aatttatctg atctatttgt attgccatct
     7621 cgctgggagg gttttgggtt agttgtcgca gaggctatgg catgtgaatg cccagtgata
     7681 gcaactgatg cagggggtgt tgctgaggtt ctttctaatg ctgattggct ggttccaatt
     7741 gctgatagca gtaaattagc tgagaaaatt gatgagtttt ttttgcttga ctcttcagaa
     7801 gttaaagata tcaaagcgaa aaacaaagac cattgtgaga accaattttc cattggggct
     7861 ataattaata attggtatgc catttataat gggataagat cgaaattata atatgaaaaa
     7921 aatatttttt gtaattacaa aatcagagat tggcggtgcg caaaagtgga ttgttgagct
     7981 atccaatatt ttaaaagaac aatatgaaat tttcattatt acatctggag atggatggct
     8041 aacatcaaaa tttgattcat ctcacgtcat tctttaccca gggttattga atcttaagag
     8101 tattttttgt ttttttaaat tggctaatat atttcggaaa tataaagctg atatcgttat
     8161 ttcaagctct gcaaatgctg gggtttatgc gaggttatct aaattcctat attctcataa
     8221 aagcatttat gtttcccatg gatggtcttg tctttataat ggcggaagat taaaaaagat
     8281 acagtgcttg gttgaaagat ttctatcatt aatttcagat aaaatactat gtgtttctga
     8341 gaatgatatg aataatgcta taaaatttat agggataaaa gaaaaaaaat tatttgtcat
     8401 tagaaatggt gttagccccc tgaaaaaaaa gaatgttatt aatgtgagaa agaaagtcct
     8461 ctttgttgga agaatgattc atcccaaaag acccgatttg ttcgtcgctg tagcagacag
     8521 ttttcctgat gtggattttt atctggtagg tgatggtcct ctaaaacaaa ggttagagac
     8581 atcctgtcaa cataataatg tttattttct tggcgagatt aatgggtttg acagttttca
     8641 gtgctatgat atttttgtac tttgttctga tagtgaaggt ttacctatgt cagcattgga
     8701 agccgcttct tctggggtgc ctattttatt aagtgatgta ggtggttgtt ctgaactaat
     8761 tactttaatg aataatggat gtagtaatgg cgagttattt tctaatgata tatcttcgtt
     8821 aagatgtaag ttaggtgctg ttttgcttga atatgataaa tatttttcat ctgctcaggt
     8881 tgtaagcggt tgctttgata taaacaatct tgctgaaaaa tatattgaat taattgaggg
     8941 tgataaatga aattagcaat cattggcggt tctggtttta tcggtacgcg tctggctacg
     9001 cagttaaaaa ataataatgt tgaatttact attgttgata aacgaaacag cgaaacgttt
     9061 ccggagtgtt ggcaatatgg agatgttact aagcctgaat cacttgaaga accattgaaa
     9121 ggcgcagatg tcatcattaa tctcgccgca gagcacaaag ataatgtcca tccaatcagc
     9181 ctttactatg atgtaaatgt ccaaggtgct aaaaatgttt gtgatgtcgc tgaaaagcta
     9241 aacatacaac atattgtatt tacttcatca gttgcagttt atggttttgt ggaaaaagaa
     9301 accggagaag atgggaagta taatcctttc aatgattatg gtaagtcaaa attagaggct
     9361 gagtttgcgt atgatgcctg gcaagcgaaa gtaccagaga gaacattggt aacagtcaga
     9421 ccaaccgttg tcttcggtga aaataatcgc gggaacgtat ataacttatt ccgccagata
     9481 gcatcaggta agtttttgat gattggccca gggctgaatc aaaaatcaat ggcgtatgtt
     9541 gaaaatatgg ccgccttctt gaggttttcg accacgatgg gagcggggcg tcatgttttt
     9601 aactatatag ataaaccaga ctttagtatg aatgatttaa ctggggtgat ttgtacagca
     9661 ttacatcgcc ataaaaacaa tattcgtatt ccttatgcgg ttggcctgtt aggtggctat
     9721 tgcttcgata tcctggcaaa ggttaccggt cgagagttcc ccgtaagcag cattcgtgtg
     9781 aagaagtttt gtgctcgtac tcagtttaag tcaaaccata tcgcgcaaac gggatttaaa
     9841 gcgccggtaa cgttagagca gggtatcgcc aatacggttc gttacgagtt tttggagaag
     9901 catgatgaca agtcttaaag ccgttatccc cgtcgccgga ctggggatga agatgttgcc
     9961 ggccaccaag gccatcccga aagagatgct gcccattgtt gataagccgc tgattcagaa
    10021 agttgtccat gagtgtttgt ccgccggggt gaaagagatc atcctggtca cccattcatc
    10081 caagaatacc attgagaacc acttcgatac ttcctttgag ctggaagcgc tgctcgaggc
    10141 gcgcgttaag cgccagttgc tggatgaggt acgttccatc tgccctcccg gggtgacgat
    10201 taacagcgtg cgtcagcata ggcagctggg tctgggacat gccattcttt gtgcccgtcc
    10261 tctggtggga gaacatcctt ttatcgtcgc gctgccggat atctggttag atgccagtac
    10321 cttcgatcct gaaacccaga atctggcagc gatggtcagg cgctttgagg agagcgggcg
    10381 cagccaggtg ctggtgcagc cgatgccgct ggaggctttg ccggagtatt cggtgattaa
    10441 ctgtgccgat gcggtgctgg aaccggggca gagtgcgtgt atgacctcga ttgtcgaaaa
    10501 acctgaggat gcggaaaagt accactccga tctgtcggcg gtcgggcgct acgtcttgtc
    10561 ggccgccatc tggccgattt tagctgccac cggctttggc gcctgggagc gcatccagct
    10621 ctccgacgct attgcaaccc tgttgcagca ccgtccggtc gaggcctacc gtcaggtggg
    10681 gctatcctat gactgtggcg ataaattggg gtatgctgag gcctttgtcg ccggtgggtt
    10741 aaatcactct cagcagggag cggcctttac cgcctggctg caggatgtgc agacgcgaat
    10801 tgaatgattc agggtattta gcatggcaat tttagtgacc ggcggcgccg gctatatcgg
    10861 ttctcatacg gtgctggcgc tgctgtcccg tggggatgag gtggtagtgc tggataactt
    10921 tgtcaacgcc tctgtgcagg cgctggcccg ggtggagcag atttgtgacc gccaacccca
    10981 cctataccgc ggtgatgtgc gcgatcgggc gctgctgcag cagatttttg cacagcacgt
    11041 cattagcgat gtgattcact tcgccggtct aaaggccgtg ggtgagtcgg tggaaaaacc
    11101 gcttgagtac tacgacaaca atgtcagcgg tacgctggtg ttgctggaag agatgcgccg
    11161 cgccggcgtg cagggcttta tctttagctc ctcggcgacg gtgtatggcg atccggagtc
    11221 agtgccgttg acggaaagca gccgtaccgg cgggacgacc aacccctatg gcacctccaa
    11281 gctgatggtc gagcagatcc tgcaggatgt atcccgcgct agacctgaga tgcggattac
    11341 cgcgctgcgc tactttaatc cggtgggagc tcatccatcg gggcttatcg gtgaagatcc
    11401 caacgggata cccaataatc tgatccctta tgttgctcag gtggcggtgg gaaaactggc
    11461 ctgcctgtcc atctttggcc atgattatcc cacacaggac ggcaccgggg tacgcgacta
    11521 tattcacgtg atggatctgg ccaccggcca ccttgccgca ctcgatcgtc gcgatgaggg
    11581 ggccagctat aaagtgtata acctggggac gggcgtcggg tattctgttt tggatatcgt
    11641 tgccgcattt gagaggtgct ccggggttac tataccttat cgctttgcac cgcgccgcgc
    11701 gggtgatatc gccgagtgtt ggtccaatcc cgccttggcg gcgcaagagt tgggttggtg
    11761 tgcgcagtat ggtctggagg cgatgatgac cgatacctgg cgctggcagc agaacaaccc
    11821 gaatggatac caggattaat tttattaaca caaaggtatg tgatgaagat agcgattagc
    11881 ggtacgggct acgtcggcct gtccaatgga attttgctgg ctcagcatca tgaggtggtg
    11941 gcgctggata ttgtggcgga aaaggtcggg atgcttaacc ggcgtcagtc accgatcgtg
    12001 gataaggaga tccaggagta cctcactaac cgctccctga atttccgcgc gacgctggac
    12061 aaggaagagg cgtaccgcgg cgctgatttt gtcattatcg cgacgccgac cgactatgat
    12121 cccaagagta attactttaa caccgccagc gtagaggcgg tgatccgcga tgtgctggcg
    12181 attaacccgg cggcggtgat gatcatcaag tcgac
//
