LOCUS       BGC0000787             13663 bp    DNA     linear   BCT 01-JAN-2005
DEFINITION  Escherichia coli serotype O127:K63(B8) O-antigen biosynthesis gene
            cluster, complete sequence.
ACCESSION   BGC0000787
VERSION     BGC0000787
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
COMMENT     ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2022-11-07 20:34:55
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 13663
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     gene            1..894
                     /gene="galF"
     CDS             1..894
                     /codon_start=1
                     /gene="galF"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1064:glucose-1-phosphate
                     adenylyl/thymidylyltransferase (Score: 185.6; E-value:
                     1.9e-56)"
                     /gene_kind="biosynthetic-additional"
                     /product="UDP-glucose pyrophosphorylase"
                     /protein_id="AAR90882.1"
                     /transl_table=11
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEIV
                     AAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNVRQ
                     GEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRSQVL
                     AKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLSADIW
                     PELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKYGLRNL
                     KEGAKFRKGIEKLLSE"
     source          1..13663
                     /db_xref="taxon:562"
                     /mol_type="genomic DNA"
                     /organism="Escherichia coli"
                     /serotype="O127:K63(B8)"
     subregion       1..13663
                     /aStool="mibig"
                     /contig_edge="True"
                     /label="Saccharide"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..13663
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            1204..2223
                     /gene="galE"
     CDS             1204..2223
                     /NRPS_PKS="Domain: NAD_binding_4 (4-173). E-value: 1.9e-14.
                     Score: 45.2. Matches aSDomain:
                     nrpspksdomains_galE_NAD_binding_4.1"
                     /codon_start=1
                     /gene="galE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) NAD_binding_4"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010:NAD-dependent epimerase/dehydratase (Score:
                     232.8; E-value: 1e-70)"
                     /gene_kind="biosynthetic-additional"
                     /product="UDP-glucose C4-epimerase"
                     /protein_id="AAR90883.1"
                     /sec_met_domain="NAD_binding_4 (E-value: 4.4e-15, bitscore:
                     45.2, seeds: 102, tool: rule-based-clusters)"
                     /sec_met_domain="Polysacc_synt_2 (E-value: 2.5e-20,
                     bitscore: 62.5, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="RmlD_sub_bind (E-value: 2.6e-12, bitscore:
                     36.1, seeds: 37, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVIFVTGGAGYIGSHTILELLNNGHDVVSIDNFVNSSIESLKRVE
                     QITNKKIISYQGDIRDKNLLDEIFSRHHIDAVIHFASLKSVGESKLKPLEYYSNNVGGT
                     LVLLECMKRYNINKMIFSSSATVYGNNSIPPHTEDRRIGETTNPYGTSKFIIEIILSDY
                     CDSDNNKSVIALRYFNPIGAHKSGMIGENPNGIPNNLVPYISKVAQNQLPVLNIYGNDY
                     PTKDGTGVRDYIHVCDLAKGHVKALEYMFLNDVNYEAFNLGTGQGYSVLEIVKMFEIVT
                     KKSIPVAICNRREGDVAESWASADLAHKKLSWKAEKKLKEMIEDVWRWQTNNPNGYKK"
     aSDomain        1216..1722
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_galE_NAD_binding_4.1"
                     /evalue="1.90E-14"
                     /label="galE_NAD_binding_4.1"
                     /locus_tag="galE"
                     /protein_end="173"
                     /protein_start="4"
                     /score="45.2"
                     /tool="antismash"
                     /translation="VTGGAGYIGSHTILELLNNGHDVVSIDNFVNSSIESLKRVEQITN
                     KKIISYQGDIRDKNLLDEIFSRHHIDAVIHFASLKSVGESKLKPLEYYSNNVGGTLVLL
                     ECMKRYNINKMIFSSSATVYGNNSIPPHTEDRRIGETTNPYGTSKFIIEIILSDYCDSD
                     NNKSVI"
     gene            2242..3258
                     /gene="wbiN"
     CDS             2242..3258
                     /codon_start=1
                     /gene="wbiN"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1045:glycosyl transferase group 1 (Score: 75.2;
                     E-value: 9.8e-23)"
                     /gene_kind="biosynthetic-additional"
                     /product="putative glycosyltransferase"
                     /protein_id="AAR90884.1"
                     /sec_met_domain="Glycos_transf_1 (E-value: 1.3e-22,
                     bitscore: 70.0, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKNVGFIVTKSEIGGAQTWVNEISNLIKEECNIFLITSEEGWLTH
                     KDVFAGVFVIPGIKKYFDFLTLFKLRKILKENNISTLIASSANAGVYARLVRLLVDFKC
                     IYVSHGWSCLYNGGRLKSIFCIVEKYLSLLTDVIWCVSKSDEKKAIENIGIKEPKIITV
                     SNSVPQMPRCNNKQLQYKVLFVGRLTHPKRPELLANVISKKPQYSLHIVGGGERLESLK
                     KQFSECENIHFLGEVNNFYNYHEYDLFSLISDSEGLPMSGLEAHTAAIPLLLSDVGGCF
                     ELIEGNGLLVENTEDDIGYKLDKIFDDYENYREQAIRASGKFVIENYASAYKSIILG"
     gene            3285..4406
                     /gene="gmd"
     CDS             3285..4406
                     /codon_start=1
                     /gene="gmd"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010:NAD-dependent epimerase/dehydratase (Score:
                     288.9; E-value: 8.4e-88)"
                     /gene_kind="biosynthetic-additional"
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="AAR90885.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 1.2e-10, bitscore:
                     30.7, seeds: 37, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAKVALITGITGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERVD
                     HIYQDPHTCNPKFHLHYGDLTDTSNLTRILCEVQPDEVYNLGAMSHVAVSFESPEYIAD
                     VDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVAKLY
                     AYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYLGNMD
                     SLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAERVGIKLRFEGTGV
                     DEKGIVVSVNGEDAPAVKPGDVIVKVDPRYFRPAEVETLLGXPTKAHEKLGWKPEITLR
                     EMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            4409..5266
                     /gene="fcl"
     CDS             4409..5266
                     /codon_start=1
                     /gene="fcl"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010:NAD-dependent epimerase/dehydratase (Score:
                     177.3; E-value: 8.2e-54)"
                     /gene_kind="biosynthetic-additional"
                     /product="GDP-fucose synthetase"
                     /protein_id="AAR90886.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 7.5e-11, bitscore:
                     31.4, seeds: 37, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSRQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDSR
                     AVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAYQNDVNKLLF
                     LGSSCIYPKLAKQPMAESELLKGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRSVMP
                     TNLYGSHDNFHPSNSHVIPALLRRFHEATAQNVPDVVVWGSGTPMREFLHVDDMAAASI
                     HVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQIIAKVVGYKGRVVFDASKPDGTP
                     QQTA"
     gene            5382..5882
                     /gene="gmm"
     CDS             5382..5882
                     /codon_start=1
                     /gene="gmm"
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="AAR90887.1"
                     /transl_table=11
                     /translation="MFLRQEDFATVVHSTPLVSLDFIVENSRGEFLLGKRTNRPAQGYW
                     FVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHYVV
                     LGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNNVHANSRAYFNHDPSAVIGLDKKDV
                     KNV"
     gene            5866..7323
                     /gene="manC"
     CDS             5875..7323
                     /codon_start=1
                     /gene="manC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) MannoseP_isomer"
                     /gene_kind="biosynthetic-additional"
                     /product="GDP-mannose pyrophosphorylase"
                     /protein_id="AAR90888.1"
                     /sec_met_domain="MannoseP_isomer (E-value: 9.9e-73,
                     bitscore: 232.8, seeds: 17, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDVPLIAVVMAGGTGSRLWPLSREHYPKQFLQLSGENTLLQSTL
                     LRLSPLSCETPLVITNEQHRFVVAEQLRQINQLSDNIILEPCGRNTAPAIALSAFTALK
                     RNEQQDPILLVLAADHVINKTDVFCNAIKNSISIVEQGNILTFGIIPDYAETGYGYIEK
                     GSIVKESQRGVGNTFYHVEQFVEKPNRSRAEEYISSGKYLWNSGMFMFKASVYLEELKK
                     YRPDIYDICEKTVSSSYHDLDFIRLSKDVFQNCPSESIDFAVMEKTKRCIVYPVDIGWN
                     DVGSWQSLWDVSDKTPTGDVCKGDILTYNTKNNYIHSESALVAAVGVEDIVIVQTKDAI
                     LVSKKSEVQDVKKIVQMLKMQERSEYLSHREEFRPWGKFDAIEQGDRYKVKKIVVKPGE
                     GLSLRMHHHRSEHWIVLSGTAKVTLNNKTFLVTANESVYIPLGATYSLENPGVIPLNLI
                     EVSSGDYLGEDDIVRQKERYKIDD"
     gene            7327..8700
                     /gene="manB"
     CDS             7327..8700
                     /codon_start=1
                     /gene="manB"
                     /product="phosphomannomutase"
                     /protein_id="AAR90889.1"
                     /transl_table=11
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLGG
                     DVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPMDY
                     NGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLREAYVDHLFGYI
                     NVKNLTPLKLVINSGNGAAGPVVDAIEARFKALRAPVELIQVHNTPDGNFPNGIPNPLL
                     PECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLEKNPGA
                     KIIHDPRLSWNTVDVVTAAGGTPIMSKTGHAFIKERMRKEDAIYGGEMSAHHYFRDFAY
                     CDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLTHPVEAINRVEQHFSR
                     EALAVDRTDGISMAFANWRFNLRSSNTEPVVRLNVESRGDQLVMENNTAIIINLLKEK"
     gene            8712..9983
                     /gene="wzx"
     CDS             8712..9983
                     /codon_start=1
                     /gene="wzx"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt"
                     /gene_kind="biosynthetic-additional"
                     /product="O-antigen flippase"
                     /protein_id="AAR90890.1"
                     /sec_met_domain="Polysacc_synt (E-value: 9.5e-17, bitscore:
                     51.1, seeds: 31, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSIISSFKNNIFLRNTMFNYIGMIWMGGGIIVLIPFYIKLLGPHG
                     WGIVSACLSIQAMLLILDAGCSQIMPRDIAKTNTPENIFFAYFWIYIAVAFVGAVFLTS
                     SAGWLAFRWFNAGHSSRELEFCIRLLAIQFLFQFANNLNIGFWNGTQKQFNTNLSQVCF
                     FSCKHCVALISLMVNPEPIVYIVSFAVVTCIEFIFNFLWITRNNIDLKGLIDNNILIQL
                     IDLVKENYIFSFGVIVGVFVSQLDKILLSHYVETQLYGVYVIVAQLGLSFLQLQYPLMK
                     SVLPALSSQGEKSIFNVLSLKKMIIMLLVLAFPIGLTIIFSSDILYLWTRNHYIANHGG
                     GALALICLSVLINIPYTFIYTNLVRLNAGGIILFSNIISALSMFILFHFMKENNSISIG
                     GMMWIAYASISFSIGGIFLLMRAR"
     gene            10232..10633
                     /gene="wbiO"
     CDS             10232..10633
                     /codon_start=1
                     /gene="wbiO"
                     /product="putative O-acetyltransferase"
                     /protein_id="AAR90891.1"
                     /transl_table=11
                     /translation="MGLGKHPVSEYISTHPFCYGKNSIHWLSKSCPPPDSHTFEETKRI
                     MIGHDVCLGANTVILDGVTVGNGAIIGAGSVVTKNIPPYAVAAGVPCKVLYYRFNDDTR
                     NKLEKMKWWEKDYEELELILSEFKYPDSE"
     gene            10641..11807
                     /gene="wzy"
     CDS             10641..11807
                     /codon_start=1
                     /gene="wzy"
                     /product="O-antigen polymerase"
                     /protein_id="AAR90892.1"
                     /transl_table=11
                     /translation="MMQITIPKSNDFRRFFNPVILILMSIVLNTFFQLYTSEEEWNSFF
                     IFNQKPDYTVFFISLVFYLFFLFGAFFAYLFNYQKHSNHRSHYLYNFSFYNGLLKFVFY
                     SNVISLIVTQYYMHEANIYNIYISGGVSAQTIESQIASSPLGIHGISVMLGYFGIIMYG
                     ISRLIKDNRAIVFISILVIIIKFISYAKLQSLLYVLLALMLFSRRRISINKGVIIGGVV
                     IFLFSFTRIIRNPEQNLSLSFDFLFRFVGGFYFGSPVVNFNYVIQNNIQDVFYFFNWFL
                     PQKIIPASPISLEFPDVTSPIGFVGSAYVSLGIFSVIYAYLVGFIIQLIYLQRNTSPFA
                     FVFQPFVIMACIFAMMYNNFVNMIFFILPLIFSYLVVIKTICARKEDV"
     gene            11808..12545
                     /gene="wbiP"
     CDS             11808..12545
                     /codon_start=1
                     /gene="wbiP"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /product="putative glycosyltransferase"
                     /protein_id="AAR90893.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 2.5e-24,
                     bitscore: 75.8, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MISVIMCINKYDSYVMPAINSILNQTYKKYELIIVANGNEASDIA
                     RVLKNNIKQTENVNIIETNIGQLAFSLNLAISKAKYDIIARMDSDDIALPNRLQKQIKY
                     LIDNELDLIGTSINLIDENDGFLGVRLYPRQKLINRYIMFRNCFAHPSIMFRKDLFLKT
                     RGYNGGFNSEDYDLWLRMRSLKPRWDNMEEPLLNYRVHSNSTQKSKLAYYECAGYSLRE
                     FLKKKSIMSFLSCTYHFIKALVK"
     gene            12553..13452
                     /gene="wbiQ"
     CDS             12553..13452
                     /codon_start=1
                     /gene="wbiQ"
                     /product="putative fucosyltransferase"
                     /protein_id="AAR90894.1"
                     /transl_table=11
                     /translation="MMYCCLSGGLGNQMFQYAAAYILKQHFPDTILVLDDSYYFNQPQK
                     DTIRHLELDQFKIIFDRFSSKDEKVKINRLRKHKKIPLLNSFLQFTAIKLCNKYSLNDA
                     SYYNPESIKNIDVACLFSFYQDSKLLNEHRDLILPLFEIRDDLRVLCHNLQIYSLITDS
                     KNITSIHVRRGDYVNNKHAAKFHGTLSMDYYISAMEYIESECGSQTFIIFTDDVIWAKE
                     KFSKYSNCLVADADENKFSVIDMYLMSLCNNNIIANSTYSWWGAWLNRSEDKLVIAPKQ
                     WYISGNECSLKNENWIAM"
ORIGIN
        1 atgacgaatt taaaagcagt tataccggta gcaggtcttg ggatgcatat gttgcctgcc
       61 actaaggcga ttcccaaaga gatgctaccg atcgtcgaca agccaatgat tcagtacatt
      121 gttgacgaga ttgtggctgc agggatcaaa gaaatcctcc tggtaactca cgcgtccaag
      181 aacgcagtcg aaaaccactt cgacacctct tatgaattag aatctctcct tgaacagcgc
      241 gtgaagcgtc aactactggc ggaagtacaa tctatctgtc cgccgggcgt gaccattatg
      301 aacgtgcgtc agggtgaacc tttaggttta ggccactcca ttttatgtgc gcgacctgcc
      361 attggtgaca atccatttgt cgtggtgctg ccagacgttg tgatcgacga cgccagcgcc
      421 gacccgctac gctacaacct tgctgccatg attgcgcgct tcaatgaaac gggacgcagc
      481 caggtgctgg caaaacgtat gccgggcgac ctctctgaat actccgtcat ccagaccaaa
      541 gaaccgctgg atcgtgaagg caaagtcagc cgcattgttg aatttatcga aaagccggat
      601 cagccgcaga cgctggactc agacatcatg gccgttggtc gatatgtgct ttctgccgat
      661 atttggccgg aacttgaacg tactcagcct ggtgcatggg gacgtattca gctgactgat
      721 gccattgccg agctggcgaa aaaacagtcc gttgatgcca tgctgatgac cggcgacagc
      781 tacgactgcg gtaaaaaaat gggttatatg caggcgtttg tgaagtatgg actacgcaac
      841 ctgaaagaag gggcgaagtt ccgtaaaggg attgagaagc tgttaagcga ataaaaatta
      901 atcatccata tatggtggat tgaataaaaa tagaacggca gtgaacattc aaaagcagag
      961 atgcttacat tataagaatg ttcctgtctt ttatttaaag tatttaataa caataagtta
     1021 ccgcaagtat cgttcggaca tagattttcc ttgtatctga cttcgtttgg ttagacaatt
     1081 ggaaaatagt gaaaagtatt aaatgcttag tacctatgat tcattttcag tgagttggta
     1141 actgttaagc caagggcggt agcgtgtgtt tttctctcaa tcctcatggt aaaggaggtt
     1201 tatatggtga ttttcgtaac aggcggtgca ggatatattg gatcccatac catacttgag
     1261 ttacttaata atggtcatga tgtcgtttcg atagataatt ttgtcaattc ctctatagaa
     1321 tcattaaaaa gagtagagca aataactaat aagaaaatta tttcttatca aggtgatatc
     1381 cgtgataaaa atctacttga tgagattttt tcaagacacc atatcgatgc tgtaattcac
     1441 tttgcatcgt taaaatctgt aggtgagtct aagttaaagc ccttagagta ttattctaat
     1501 aatgttggtg gaactttagt attacttgaa tgcatgaaga gatataacat taataaaatg
     1561 atatttagct cttctgctac tgtttatggg aataacagta tccctcccca tacggaagat
     1621 agacgaattg gtgaaactac aaacccatat gggacatcga aatttataat agaaataatt
     1681 ttgagtgatt attgtgatag tgataataat aaatcagtaa ttgcactgcg ttactttaat
     1741 ccaatcggag cacataagtc cgggatgatt ggtgaaaatc ctaacgggat tcctaataat
     1801 ctggttcctt atatatctaa agttgcacaa aatcaacttc ctgtattaaa tatttatggc
     1861 aacgattatc caactaaaga tggtacagga gtaagagact atatacatgt ctgtgatttg
     1921 gctaaagggc atgttaaagc attagaatat atgtttttaa atgatgtcaa ttatgaagct
     1981 tttaatttag gcactggtca aggttattct gttttagaga ttgtaaaaat gtttgagata
     2041 gtcactaaaa agagtatacc tgttgctatt tgtaatagac gtgaggggga tgttgcggag
     2101 tcatgggcgt ctgctgattt agcacataaa aagctttcct ggaaagcgga aaaaaaattg
     2161 aaagaaatga tcgaagatgt atggcgttgg caaacaaaca atccaaatgg atataaaaaa
     2221 taatttttgg aattttgata tatgaaaaat gttggtttta ttgttacaaa atcagaaatt
     2281 ggtggtgcac aaacatgggt aaatgaaata tctaacctta ttaaagagga atgtaatata
     2341 tttcttatta catctgaaga aggatggctc acacataaag atgtctttgc cggagttttt
     2401 gtcataccag gtattaaaaa atattttgac ttccttacat tgtttaaatt gagaaaaatt
     2461 ttaaaagaaa ataacatttc aacgttaata gcaagttctg ctaatgccgg agtttatgcc
     2521 aggttagttc gattactagt cgactttaaa tgtatttatg tttcgcatgg atggtcttgt
     2581 ttatataatg gtggtcgcct aaaatcaatt ttttgcattg tggaaaaata cctttcttta
     2641 ttaactgatg ttatatggtg tgtttccaaa agtgatgaaa aaaaggcaat tgagaatatt
     2701 ggtataaaag aaccaaagat aatcacagta tcgaattcag tgcctcagat gccgagatgt
     2761 aataataaac aactccagta taaggttctg tttgttggta ggttaacaca ccctaagcgc
     2821 cccgagttgt tagcgaatgt aatatcgaaa aagccccagt atagcctcca tatcgtagga
     2881 gggggggaaa ggttagaatc attgaagaaa caattcagtg aatgtgaaaa tattcatttt
     2941 ttgggtgagg tcaataattt ttataactat catgagtatg atttattttc actgatatcc
     3001 gatagtgaag gtttgcctat gtcaggcctt gaggctcaca cagctgcaat accactcctg
     3061 ttaagtgatg tgggcggatg ttttgaatta attgagggta atgggttact tgtggaaaat
     3121 actgaagacg acattggata taaattggat aaaatattcg atgactatga aaattatcgg
     3181 gaacaggcaa ttcgtgcttc cgggaaattt gttatcgaga actatgcttc agcatataaa
     3241 agcattattt taggttgagt atttttaaga acaggaaatg taaaatggct aaagtcgctc
     3301 tcattactgg cataaccgga caagacggtt cttacctggc agagtttctg ctggaaaaag
     3361 gttacgaggt gcatggtatt aagcgtcgtg catcgtcatt caacaccgag cgcgtggatc
     3421 acatttatca ggatccgcac acctgcaacc cgaaattcca tctgcattat ggcgatctga
     3481 cagatacctc caacctgacg cgcattttgt gtgaagtgca gccggatgaa gtgtacaacc
     3541 tgggcgcaat gagtcacgtt gcggtttctt ttgagtcacc ggaatatatc gctgacgtcg
     3601 atgcgatggg tacgctgcgc ctgctggagg cgatccgctt cctcggtctg gaaaagaaaa
     3661 cccgttttta tcaggcttcc acctctgaac tgtacggtct ggtgcaggaa attccgcaga
     3721 aagaaaccac gccgttctac ccacgctctc cgtatgcggt cgccaaactg tatgcctact
     3781 ggatcaccgt taactaccgc gaatcctacg gcatgtacgc ctgtaacggt attctcttca
     3841 accatgaatc cccgcgccgc ggcgaaacct tcgttacccg caaaatcacc cgcgcaatcg
     3901 caaacatcgc ccaggggctg gaatcgtgcc tgtacctcgg caatatggat tccctgcgtg
     3961 actggggcca tgccaaagac tacgtaaaaa tgcagtggat gatgctgcaa caggaacagc
     4021 cggaagattt cgttatcgcg accggcgttc agtactccgt gcgtcagttc gtggaaatgg
     4081 cggcggagcg agtcggaatc aaactgcgtt ttgaaggcac aggtgtagat gagaagggga
     4141 tagtggtttc tgttaatggt gaggatgctc cagcagttaa acctggagat gtaatcgtta
     4201 aggttgatcc gcgctacttc cgtccggctg aagttgaaac gctgctcggc gnaccgacca
     4261 aagcgcacga aaaactgggc tggaaaccag aaatcaccct cagagagatg gtgtctgaaa
     4321 tggtggctaa tgacctcgaa gcggcgaaaa aacactctct gctgaaatct cacggctacg
     4381 acgtggcgat cgcgctggag tcataagcat gagtagacaa cgcattttta tcgctggcca
     4441 tcgcggaatg gtcggttctg ccatcaggcg gcagctcgaa cagcgcggtg atgtggaact
     4501 ggtattacgc acccgcgacg agctgaacct gctggacagc cgcgcggtgc atgatttctt
     4561 tgccagcgaa cgcattgacc aggtctatct ggcggcggcg aaagtgggcg gcattgttgc
     4621 caacaacacc tatccggcgg atttcatcta ccagaacatg atgattgaga gcaacatcat
     4681 tcacgccgcg tatcagaacg acgtgaacaa actgctgttt cttggatcgt cctgtatcta
     4741 cccgaaactg gcaaaacagc cgatggcaga aagcgagctg ttgaagggca cgctggagcc
     4801 gactaacgag ccttatgcta ttgccaaaat cgccgggatc aaactgtgcg aatcttacaa
     4861 tcgccagtac ggacgcgatt accgctcagt catgccgacc aacctgtacg ggtcgcacga
     4921 caacttccac ccgagtaact cccatgtgat acctgcattg ctgcgtcgct tccacgaggc
     4981 gacggcacag aatgtaccgg acgtggtggt gtggggcagc ggtacaccta tgcgcgaatt
     5041 tctgcacgtc gatgatatgg cggcggcgag cattcatgtc atggagctgg cgcatgaagt
     5101 ctggctggag aacacccagc cgatgttgtc gcacattaac gtcggcacgg gcgttgactg
     5161 cactatccgc gagctggcgc aaatcatcgc caaagtggtg ggttacaaag gtcgggtggt
     5221 ttttgatgcc agcaaaccgg atggtacgcc gcagcaaact gcttgatgtg acgcagcctg
     5281 catcagcttg gctggtatca cgaaatctca ctggaagcag gggccttgcc agcacttacc
     5341 agtggttcct tgagaatcaa gaccgctttc gggggtaata gatgttttta cgtcaggaag
     5401 actttgccac ggtagtgcac tccaccccgc ttgtctctct cgactttatt gtcgagaaca
     5461 gtcgcggcga gtttctgctt ggcaaaagaa ccaaccgccc ggcgcagggt tactggtttg
     5521 tgccgggagg gcgcgtgcag aaagacgaaa cgctggaagc cgcatttgag cggctgacga
     5581 tggcggaact ggggctgcgt ctgccgataa cagcaggcca gttttacggt gtctggcagc
     5641 acttttatga cgataacttc tccggcacgg atttcaccac tcactatgtg gtgctcggtt
     5701 ttcgcttcag agtagcggaa gaagagctgt tactgccgga tgagcagcat gacgattacc
     5761 gctggctgac gccggacgcg ctgctcgcca gtaacaatgt tcacgctaac agccgagctt
     5821 attttaatca tgatccaagt gctgttattg gtttagataa aaaggatgtc aaaaatgtct
     5881 gatgtaccgt tgattgctgt tgtaatggct ggtggtacgg gtagccgtct ttggcccctc
     5941 tcaagagagc actatccgaa gcaatttctg caattatcag gtgagaatac tttattacag
     6001 tcaactttat tacgactatc cccgctttct tgcgaaactc cattagtaat aactaacgag
     6061 cagcatcgtt ttgtggtcgc agaacaattg cgtcaaataa accaattaag cgataacatt
     6121 attcttgaac catgtggaag aaatactgca ccagctatag cactttcagc atttacagcc
     6181 ttaaagcgta atgaacaaca agatcctata cttttagttc ttgcagctga ccatgttata
     6241 aacaaaacag atgttttttg taatgctatt aaaaattcca tttcaattgt tgaacaagga
     6301 aatattttaa catttggtat tattcctgat tatgcagaaa ctggttacgg ttatatagaa
     6361 aaagggagta tagttaaaga atcacaacgt ggagttggta atacattcta tcacgtggaa
     6421 caatttgttg aaaaacctaa tcgctcaagg gctgaagagt atatctcctc aggtaaatat
     6481 ctctggaata gtgggatgtt tatgttcaaa gcatcagtat atttagagga gttaaaaaaa
     6541 tacagacctg atatatacga tatatgtgaa aaaacagttt cttcgtccta ccatgatctt
     6601 gattttatac gactttcgaa agatgttttt caaaattgtc catcggaatc aattgatttt
     6661 gcagtaatgg aaaaaacaaa gcgttgtata gtctatcccg ttgatattgg ttggaacgat
     6721 gttggatctt ggcaatcatt gtgggacgtt agtgataaaa ctcctacagg ggacgtttgc
     6781 aaaggtgata tcctgactta taatacaaaa aataattata ttcattcgga gtcagcctta
     6841 gtagccgctg taggtgtgga ggatattgtt attgttcaga cgaaagatgc aattctagtt
     6901 tcaaaaaaat ccgaagttca ggatgttaag aaaattgttc aaatgcttaa aatgcaagaa
     6961 cgttctgaat atctatcaca tcgcgaggaa tttcgtcctt ggggaaagtt tgatgctata
     7021 gaacagggag atcgatataa ggtcaaaaag attgtcgtta aaccagggga agggctatcc
     7081 ttacgtatgc atcatcatcg ttcagagcat tggattgtcc tttcaggtac agcaaaagta
     7141 acgttaaata ataaaacttt cttagttact gctaatgaat ccgtttatat tcctctgggt
     7201 gctacttata gccttgaaaa tccaggtgta attcctctca atcttattga agttagttca
     7261 ggagattatt taggtgagga tgatattgtt cgtcaaaaag aacgttacaa aatagatgac
     7321 taataaatga aaaaattaac ctgctttaaa gcctacgata ttcgcggaaa attaggcgaa
     7381 gaactgaatg aagatattgc ctggcgtatt gggcgtgcct atggcgaatt tctcaaaccg
     7441 aaaactattg ttttaggtgg tgatgtccgc ctcaccagcg aaaccttaaa actggcgctg
     7501 gcgaaaggtt tacaggatgc gggcgtcgat gtgctggata tcggcatgtc cggcaccgaa
     7561 gagatctatt tcgctacgtt ccatctcggc gtggatggcg gcatcgaagt tactgccagc
     7621 cataacccga tggattacaa cggcatgaag ctggtgcgcg agggggctcg cccgatcagc
     7681 ggtgataccg gactgcgcga tgtccagcgt ctggcagaag ccaacgactt ccctcccgtc
     7741 gatgaaacca aacgcggtcg ctatcagcaa atcaatctgc gtgaagctta cgttgatcac
     7801 ctgttcggtt atatcaacgt caaaaacctc acgccgctca agctggtgat taactccggg
     7861 aacggcgcgg cgggtccggt ggtggacgcc attgaagccc gctttaaagc cctccgcgca
     7921 ccggtggagt taatccaagt gcataacacg ccggacggca atttccccaa cggtattcct
     7981 aacccgctac tgccggaatg tcgcgacgac acccgcaatg cggtcattaa acacggcgcg
     8041 gatatgggca ttgcttttga tggcgatttt gaccgctgtt tcctgtttga cgaaaaaggg
     8101 cagtttattg agggttacta cattgtcggt ttgctggcag aggcgttcct cgaaaaaaat
     8161 cccggcgcga agatcatcca cgatccacgt ctctcctgga acaccgttga tgtggtgact
     8221 gccgcaggcg gtacgccaat aatgtcgaaa accggacacg cctttattaa agaacgtatg
     8281 cgtaaggaag acgctatcta cggtggcgaa atgagcgccc accattactt ccgtgatttc
     8341 gcttactgcg acagcggcat gatcccgtgg ctgctggtcg ccgaactggt gtgcctgaaa
     8401 gggaaaacgc tgggcgaact ggtgcgcgac cggatggcgg cttttccggc aagtggtgag
     8461 atcaacagca aactcacgca ccccgttgag gcgattaacc gcgtggaaca gcattttagc
     8521 cgtgaggcgc tggcggtgga tcgcaccgat ggcatcagta tggcctttgc caactggcgc
     8581 tttaacctgc gctcctccaa caccgaacct gttgtgcgtt tgaacgttga atcaagaggt
     8641 gatcagctcg tgatggaaaa taatacggca attattatta atttattaaa agaaaaataa
     8701 aaaccatcta tatgtcaata atatcctctt ttaaaaataa tatattctta cgaaacacaa
     8761 tgtttaatta cattggtatg atttggatgg ggggggggat aatagtcctt ataccctttt
     8821 atatcaagct actaggtccc catggatggg gtatcgtctc tgcttgttta agtatccagg
     8881 ctatgttact tattctggat gctgggtgtt cgcagattat gcctcgcgat attgctaaaa
     8941 cgaatacgcc agaaaacata ttttttgcat atttttggat atatattgct gttgcttttg
     9001 ttggggcggt ttttctaacc agttcggctg gttggttggc atttagatgg tttaatgcag
     9061 ggcatagcag tagggagtta gagttttgta taagattgct tgcaattcaa ttcctatttc
     9121 aatttgcaaa taatctcaat ataggttttt ggaatggtac tcaaaagcaa tttaatacta
     9181 atttaagcca agtgtgtttt ttttcatgta aacattgtgt cgcgctgatt tcattgatgg
     9241 taaatccaga acctatagta tatatagttt ccttcgcagt ggttacctgt atagaattta
     9301 tttttaattt tttatggatt actcgcaata atatagatct taaaggctta atagataata
     9361 atatattgat tcaattaatt gatttggtta aagagaacta tatattttca tttggtgtga
     9421 ttgttggtgt gtttgtttca cagttagata aaatattatt atcacactac gtggaaacac
     9481 agttatatgg agtatatgtt attgttgcac aactgggatt atctttttta cagctacaat
     9541 atcctttgat gaagtcagtg ttaccagctt tatcatcaca aggggaaaag agtatattta
     9601 atgttctatc gttaaaaaaa atgataatta tgctattggt attagctttc cctattggtc
     9661 tgacaataat attttcatct gatattttat atttatggac aagaaatcat tatatagcta
     9721 atcatggagg gggggcttta gctcttattt gcctctcagt tttaataaat ataccatata
     9781 catttatcta tacaaacctt gttcgattaa acgcaggggg aattatactg ttcagtaata
     9841 ttatttctgc attaagcatg tttattttat tccatttcat gaaagaaaat aactctattt
     9901 caattggcgg catgatgtgg atagcatatg caagtatatc tttttcaatt ggcggaatat
     9961 ttttattaat gagagcaaga tgaatataat aaaagaatat atactgttac ctatatgcta
    10021 tttaattaaa aacattattc tatgttttca aggcgttagt gtttatcccg gagcgagaat
    10081 aaggcatgta acatttgagg agaatatata agtgtataac aatacattgc tttataattc
    10141 aaatattgga cgtcatactt atattcagag gaattgtaca atattgaaca cgtcaatcgg
    10201 tcggtattgt tctattgcag aaagtgtgaa aatgggatta ggtaaacatc ctgtgtcgga
    10261 atatatatcg acgcatccat tctgttatgg gaaaaattca atacattggc tttctaaaag
    10321 ctgtccacct ccggattcgc atacatttga agaaaccaaa agaattatga ttggtcatga
    10381 tgtatgtttg ggtgcgaata cggttatatt ggatggagta actgtaggta atggcgctat
    10441 aattggggct ggctcagttg ttactaagaa tatacctcct tatgctgttg ctgcaggggt
    10501 tccttgtaaa gtactttatt atcgttttaa tgatgataca agaaataagt tggaaaaaat
    10561 gaaatggtgg gaaaaagatt atgaagaact agaactgatt ctttcagaat ttaaatatcc
    10621 agactcggaa taagattaag atgatgcaga taacaattcc aaaaagtaat gattttagac
    10681 gtttctttaa tcccgttata ttaattttaa tgtcaatcgt tttaaacact ttttttcaat
    10741 tatacaccag tgaagaagaa tggaattcat tctttatatt taatcaaaaa cctgattata
    10801 cggtgttttt tatatcttta gttttttatt tgttttttct ttttggggct ttttttgcgt
    10861 atttatttaa ttatcaaaaa catagcaacc atagaagcca ctacttatac aacttttctt
    10921 tttataatgg gttattaaag tttgtttttt attcaaatgt aataagttta attgtaaccc
    10981 agtattacat gcacgaagct aatatatata atatatatat tagcggtggt gtctctgctc
    11041 aaacaataga gtctcagatt gcttcatctc cgctgggaat acatggaata tctgtaatgt
    11101 tgggctattt tggtattatt atgtatggta tatctcgttt gataaaagat aatcgagcta
    11161 ttgtttttat aagtatattg gttataataa tcaaatttat tagttatgcg aaattgcaat
    11221 ctttattata tgttttatta gctttgatgc tattttccag acggagaata tctattaata
    11281 aaggagttat aataggaggt gtagtcattt ttttattttc atttacgcgc ataattcgaa
    11341 atcctgaaca aaatttatca ttgagttttg attttttatt tagatttgtt ggcggttttt
    11401 atttcggttc acctgttgtt aattttaact atgttattca aaataatata caggatgtgt
    11461 tttatttctt caattggttt ttacctcaaa aaatcattcc agcgtcacca attagcttgg
    11521 agtttccaga tgttacctca cctataggtt ttgtaggaag tgcttatgtt tcattgggta
    11581 tattcagcgt aatatatgcc tatttggttg ggttcataat tcaattaata tatttacagc
    11641 gaaatacgtc tccttttgca tttgtttttc aaccatttgt cattatggcc tgcatatttg
    11701 cgatgatgta taataatttt gtaaatatga tatttttcat attgccgctt atattttcat
    11761 atttagtggt tataaaaact atatgcgcta gaaaggaaga tgtttaaatg atttctgtaa
    11821 ttatgtgtat aaataaatat gattcatatg taatgcctgc gataaatagt atattgaatc
    11881 aaacttataa aaaatatgag ttaataattg ttgctaatgg caatgaagcc agtgacattg
    11941 cccgtgtctt aaaaaataac ataaaacaaa cagagaatgt taatattatt gaaaccaata
    12001 tagggcaatt ggctttttct ttaaatttgg caatatccaa agctaaatat gatatcatcg
    12061 cgaggatgga ttctgatgac atagccttac ctaaccgact tcaaaagcaa attaaatatc
    12121 ttatagataa tgaacttgat ttaattggaa cctcaataaa tctaattgat gaaaatgatg
    12181 gttttttagg tgtaaggctt taccccagac aaaaattaat aaatagatat ataatgtttc
    12241 gaaactgttt cgcacatcct tcaataatgt tcagaaaaga tttgtttctt aaaacaagag
    12301 ggtataatgg tggttttaac tctgaagatt atgatttgtg gttaagaatg cgaagcttaa
    12361 aaccacgttg ggataatatg gaagagcctt tgttaaatta ccgtgttcac tcaaattcaa
    12421 cacagaagtc taaactggca tattatgaat gtgctgggta ttctttgcga gaatttctta
    12481 aaaagaaaag tataatgagt tttctttctt gtacatatca ttttattaaa gcattagtta
    12541 aataaggact taatgatgta ttgctgtcta tccggtgggt taggtaatca aatgttccaa
    12601 tatgctgcag cctatatcct taagcaacat tttcctgata ctatattagt gcttgatgat
    12661 agttattatt tcaaccagcc tcaaaaagat actatacgac atcttgagct tgatcaattt
    12721 aaaattattt ttgataggtt tagttctaaa gatgaaaaag taaaaataaa tcgtttgagg
    12781 aaacataaaa aaataccact gcttaacagt tttcttcagt tcactgcaat taaactgtgt
    12841 aataaatatt cattaaatga tgcttcttat tacaatccgg aatcaattaa aaacattgat
    12901 gttgcgtgtc tattctcatt ttaccaagat tctaaattat taaatgaaca tagggattta
    12961 attttgcccc tttttgaaat acgtgatgat cttcgcgtgt tatgtcataa cctacaaata
    13021 tattccttaa ttactgactc taagaatata acgtcaattc atgtgcgacg tggagattat
    13081 gtaaataata aacatgctgc aaaatttcac ggcactctaa gtatggatta ttatattagt
    13141 gcaatggaat acattgaaag tgaatgtggc tctcagacat ttataatctt tacagatgat
    13201 gtcatatggg caaaggaaaa attctcaaaa tatagcaatt gcttggttgc tgatgctgat
    13261 gaaaacaaat ttagtgtaat tgatatgtat ttaatgtcat tgtgtaataa taatattatt
    13321 gcaaacagca catatagttg gtggggggca tggctaaaca ggtcagaaga taagctagtc
    13381 atcgcgccta aacaatggta catatccgga aatgaatgct ctcttaaaaa tgaaaactgg
    13441 atagcaatgt agagttttca ttgtttcaat tctcattgac attacatgtg agatttacct
    13501 gtatttatat taacatctgc atcatatcca agcctgcaca cttggcggta accacccctg
    13561 acaggagtaa acaatgtcaa agcaacagat cggcgtagtc tggtatggca gtgatggggc
    13621 tgcaaccttg ctgctcaaca tcgaaagccg tggttatacc gtc
//
