LOCUS       BGC0000789             18772 bp    DNA     linear   BCT 30-NOV-2006
DEFINITION  Escherichia coli O24 O antigen gene cluster, complete sequence.
ACCESSION   BGC0000789
VERSION     BGC0000789
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 14:12:45
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..18772
                     /db_xref="taxon:562"
                     /mol_type="genomic DNA"
                     /note="serogroup: O24"
                     /organism="Escherichia coli"
     subregion       1..18772
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000789"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..18772
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            1096..1875
                     /gene="wfaM"
     CDS             1096..1875
                     /codon_start=1
                     /gene="wfaM"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /note="glycosyl transferase"
                     /product="WfaM"
                     /protein_id="ABB29900.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 2.7e-38,
                     bitscore: 120.8, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNKETVSVIMPVYNGARTIISSVESIINQSYQDFVLYIIDDCSTD
                     DTFSLIFNRYKNNQKIRMLRNDSNLGVAESRNVGIKAATGKYISFCDADDLWHQNKLER
                     QIELFNNECVDVVCSNYYVVNINGDVIGEVKAPHIITYRKMLMKNHIGNLTGIYNANKL
                     GKFYQKNIGHEDYLMWMEIINKTNVAICIQDNLAYYMRTSNSLSANKVKTAKWMWNIYR
                     QCLHLSFTKSLYCFFMYAFNGVIKEIMHLPLKALRKK"
     misc_feature    1096..17325
                     /note="O antigen gene cluster"
     gene            3584..4204
                     /gene="nnaD"
     CDS             3584..4204
                     /codon_start=1
                     /gene="nnaD"
                     /note="N-acetylneuraminic acid synthetase"
                     /product="NnaD"
                     /protein_id="ABB29901.1"
                     /transl_table=11
                     /translation="MKKKLIIIGAGGFAKAVIDSLDHACYEIHGFIDSFKCGEHQGYKI
                     ISRTIDSLDEPHKYCYFIAIGEPYYRAIWLETINAMKLETINVIDRTAIISKGAILGSC
                     IYVGKMAIVNCDSHLEDGVVINTRALVEHGNYISYCSNISTNVVLNGDVFVGRKCFIGS
                     CTVVNGQLKIGDSSIVGSGSVVIRNVDENVVVAGSPTRLIRKR"
     gene            4209..5246
                     /gene="nnaB"
     CDS             4209..5246
                     /codon_start=1
                     /gene="nnaB"
                     /note="N-acetylneuraminic acid synthetase"
                     /product="NnaB"
                     /protein_id="ABB29902.1"
                     /transl_table=11
                     /translation="MSSVYIVAEIGCNHNGDYNLAKLMIDEAYSAGVDAVKFQTFKADS
                     LISKYAPKAEYQIKTTGNSESQLEMTRKLELSCEEYIKLEEYSKSLGLDVFSTPFDLES
                     IEFLYTRKQTTWKIPSGELLNLPYLEKIALLPGDKKIILSTGMATLAEIKQALKIFTDN
                     GVKAEDITILHCNTEYPTPYEDVNLNVLAGLKKIFQEYNIGFSDHSPGYFAGIAAVPYG
                     ITFIEKHFTLNKDFEGPDHKASITPDELRLLCNGIRAIERALGSFDKRVTNSERKNKLV
                     ARKSIVAKRDIKKGEIFTTDNITTKRPGNGISPMYWYEVLGKKAEQDFLEDQLIKVSGF
                     EEQEI"
     gene            5249..6508
                     /gene="nnaC"
     CDS             5249..6508
                     /codon_start=1
                     /gene="nnaC"
                     /note="CMP-N-acetylneuraminic acid synthetase"
                     /product="NnaC"
                     /protein_id="ABB29903.1"
                     /transl_table=11
                     /translation="MRKKIAIIPARSGSKGLPNKNILMLFDKPLIAYTIEAAIKSELFE
                     KVIVSTDSCEYKDIALRHGAEVIMRDKSLASDTATSYMVVKDVLEKNISCDYFVLLQPT
                     SPFRNYKHIREAVEKFENTDDIDFLVSMVKTNKSADLINQLAPNDNLKYFNADFANYRR
                     QNQENYCPNGAIFIGNVDAYLRQKHFFGERSIAYVMDRIDSLDIDDKLDFEFAISLMLK
                     KNRMKDLQKEILKRIKEKEFFFDKIKPVTLVGHSIIDYWNVTHLNGREVNNLGIAGINS
                     KEYYDLIIKNNIIKFGDTVILMLGTNDIVVDDWSVDFTILWIEKIVKHIKKINHNASIY
                     ILSIPPVLGRIDRNNETIRKLNSELSKFFENYQNVIFITLSKVFYDQYGNLKENFTYDG
                     LHFSQLAYNQLTKEIEEKIK"
     gene            6505..7692
                     /gene="nnaA"
     CDS             6505..7692
                     /codon_start=1
                     /gene="nnaA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Epimerase_2"
                     /gene_kind="biosynthetic-additional"
                     /note="UDP-N-acetylglucosamine-2-epimerase"
                     /product="NnaA"
                     /protein_id="ABB29904.1"
                     /sec_met_domain="Epimerase_2 (E-value: 1.9e-85, bitscore:
                     276.1, seeds: 187, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKILYITGSRAEYGIMKRLLRKLKDNEHIELSIIATGMHCDAKY
                     GYTYQNIIDDGFEVKELFDINIESSTNSGIISTMSRAQNLFGAYFEEYKYDAIIILGDR
                     YEMLSVAIAASIHGIPIIHLHGGEQTLGNYDEFIRHCITKMSHLHLVATEKYRERVIQL
                     GELPSWVINIGAMGAENTVSLSIPEHKELQSKYENSLSNYFMILFHPETLTKNKSVHDQ
                     IKELIKALDIAHQKYHCNFIFIGTNADTNSDIITKEILTFCESYNFPYLLSLPTQDYLG
                     LCKYSLGLIGNSSSGLIEIPSLGVPTINIGSRQAGRERGKSVIDVECCYTDILEKIDDV
                     ISNRIDRTYYNPYYKENSAEIAMSSIISFLDWISDNKNRIKKFYDIPKVTTLINS"
     gene            9380..10387
                     /gene="wfaN"
     CDS             9380..10387
                     /codon_start=1
                     /gene="wfaN"
                     /note="CMP-N-acetylneuraminic acid transferase"
                     /product="WfaN"
                     /protein_id="ABB29905.1"
                     /transl_table=11
                     /translation="MKSNVYICKTTWEIFVSILLASDIYYDKKIKSVFVIEVAEENINY
                     LSKLSDFFFVSKVIPVRFSSNKYVKALCFHYRVRYYLPKMLGEYLKSDVRVNLFIDQGV
                     FGQYFIKNNKDVYLYEHGNGNYCVGPYPNYKMIKKFLGITEGYGRHSRVKRIYLQHPEK
                     APKDIIHKVTGFSIKNLYNKLFLKEQQNLLSFFGIHKINKTSDVIILTQPISEDGFYSE
                     REKIEIYREIIDSRFPSDKSRVVIKPHPRDKTNYGKYIPDVMLLPRSFPMEILNFTNIR
                     FIKAITICSSAVYNFEYDISVDYIGTLRYPKLIKALGRLPAQYVHKDNYKNIQY"
     gene            10411..11463
                     /gene="wzy"
     CDS             10411..11463
                     /codon_start=1
                     /gene="wzy"
                     /note="O-antigen polymerase"
                     /product="Wzy"
                     /protein_id="ABB29906.1"
                     /transl_table=11
                     /translation="MIPYVMFYCSSLVLVVLSLVLKKYDWFWLLFLLFFSAVFVGLRVD
                     VGADYTEYAQIYNQSGNITNFELGFDIIFNYGKRLGYDYVFVSLFFFLLTTLFFIYSIK
                     ELNYKTLIYFCFLLFMFVPLTSTIRQGLAIPFFVMCILNSDRPKVYFTSIALGCLFHYS
                     ILFMFFFFWVRHIKQSYCRAFLIVLLFSLLSIFNIVEYIIMLLNYMPGLGAISTKLLMY
                     TQRYQVEFSMNSFVYKLAGLLLITMFMPFIKVNEKLRCSYNLYFITVCIFILLKDNAVL
                     TNRLTYATNISLVTFFSLLLSMIRGFYKKAIVSILFVFYFSFSYFKFTATDLRHSVESA
                     YMPYKSLLIN"
     gene            11479..12672
                     /gene="wzx"
     CDS             11479..12672
                     /codon_start=1
                     /gene="wzx"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt"
                     /gene_kind="biosynthetic-additional"
                     /note="O-antigen flippase"
                     /product="Wzx"
                     /protein_id="ABB29907.1"
                     /sec_met_domain="Polysacc_synt (E-value: 1.7e-11, bitscore:
                     33.3, seeds: 31, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLLKSRFSYFATELINKCVPFFLLPYVSSAVGVEVYGKIELFTT
                     NYIILSFLFALGVEGWMNAHYFKMAESDFITVLSSFYLLSFLIFVICMAISLPYSYYCS
                     ALLFVGYVQSLLNITTLHLRLKGKYLNAGFLLLLSSIVNAVMVYASFDWFGKTYESRIN
                     SFLFSSIIILVFIVFLSIRDKNIKIKIDFSFLNRDILLFCLPLCLTMIINWVKGNIDKY
                     FIANLLDLKSLGIYAVAYQLASVINVVGIILNRTLQADLLKSMADGFYQTKKIIFTFLF
                     ILCVFFFLYIVAVYCGFSYVFSIDYSSSRNIALLLMIGFLLLSFSSFLINFLLFYRRPR
                     LILFQTFLITMIHVMLSFILIKKYALLGVLLTQFVTSLLTFVSTLLVCHILTKSSQK"
     gene            16255..17325
                     /gene="wfaO"
     CDS             16255..17325
                     /codon_start=1
                     /gene="wfaO"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1045:glycosyl transferase group 1 (Score: 117.4;
                     E-value: 1.5e-35)"
                     /gene_kind="biosynthetic-additional"
                     /note="glycosyl transferase"
                     /product="WfaO"
                     /protein_id="ABB29908.1"
                     /sec_met_domain="Glycos_transf_1 (E-value: 1.1e-39,
                     bitscore: 125.0, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKITFVIADITFVGGIERVNTLLANKFFSEGYNVEIISLYNTNK
                     KVNYELNNCIKVNFVKDNSYSGAPGSFGRLKQHISSQFALFKAIKNSNADFYIVNSFPM
                     AFLSFFNVFSNKKYVVIEHVHYDYYGVAIKFFRNLLYKFYYKVVCVNESDLSKFSKHLN
                     NAVKINNPLSFSSSEITCLSEKKIIAVGRLEYQKGFDILIDIFARVNKEHPGWELHIYG
                     VGTCEEFLRDKINQYKLNNIKLMGCVDNIQLYYPKYSVFVFSSRFEGFGMVLLEAMECG
                     LPCISFDCPTGPSEILGNGQYGILVENGNVTKFSAELADLMSDKEKKIKLSNISKTRAK
                     EFHIDKIFSEWRKLIN"
ORIGIN
        1 attgtggctg cagggatcaa agaaatcctc ctggtaactc acgcgtccaa gaacgcggtc
       61 gaaaaccact tcgacacctc ttatgaatta gaatctctcc ttgagcagcg cgtgaagcgt
      121 caactactgg cggaagtgca atctatctgc ccgccgggcg tgactattat gaacgtgcgt
      181 cagggcgaac ctttaggttt aggtcactcc attttgtgtg cacgacccgc cattggtgac
      241 aacccatttg tcgtggtact gccagatgtt gtgatcgacg acgccagtgc cgacccgctg
      301 cgctacaacc ttgctgccat gattgcgcgt ttcaatgaaa cgggccgtag ccaggtgctg
      361 gcaaaacgta tgccgggtga cctttctgaa tactccgtta ttcagaccaa agagccgctg
      421 gatcgtgaag gcaaagtcag ccgcattgtt gaatttatcg aaaaaccgga tcagccgcag
      481 acgctggact cagacatcat ggccgttggt cgctatgtgc tttctgccga tatttggccg
      541 gaacttgaat gcactcagcc tggtgcatgg gggcgtattc agctgactga tgccattgcc
      601 gggctggcga aaaaacagtc agttgacgcc atgctgatga ctggagacag ctacgactgc
      661 ggtaaaaaaa tgggctatat gcaggcgttt gtgaagtatg gattgcgcaa cctgaaagaa
      721 ggggcgaagt tccgcaaatg tattgagata attttaagtg aataaaaatc tgattgggcg
      781 taacggcagc cgagaaattt atagtagcag taaacttctt gttgtagaag tatagtataa
      841 tgaatatttc tgttagtgag ttttttaatt aatcatagag gtaatgggca taaaatcaat
      901 atgctagcga ttttttttca gaggtattgt ttctcctttt gaataatcag ataatgaata
      961 ctggatataa tgtttttaca tgagctgagt atgctctttg caacataact ttttaattag
     1021 tgcactggta gctgttaagc caggggcggt agcgtgctta agtaatgtta atgaaagcat
     1081 ataagaaatt ttcaaatgaa taaagaaact gtttcagtta ttatgcctgt ctataatggg
     1141 gctcgaacta taatttcatc agtagaatcg attataaatc aatcatatca agactttgtt
     1201 ttatatatca ttgacgattg tagcaccgat gatacatttt cattaatttt taatcgatat
     1261 aaaaacaatc aaaaaataag gatgttgcgg aatgactcta atttaggtgt ggcggaaagt
     1321 agaaatgttg gaataaaagc agcaacagga aaatatattt ctttttgtga tgcggatgat
     1381 ttatggcacc agaataaatt agaacgacaa atcgaattat tcaacaatga atgtgttgat
     1441 gtggtatgtt ctaattatta tgtggtgaat attaatgggg atgttattgg tgaggttaaa
     1501 gcacctcata tcataactta cagaaaaatg cttatgaaaa atcatatagg gaatttaact
     1561 ggtatttata atgctaacaa attgggtaaa ttttatcaaa aaaatattgg ccacgaggat
     1621 tatttaatgt ggatggagat aataaacaaa acaaacgttg ccatttgtat tcaagacaat
     1681 ctcgcttatt acatgcggac aagtaattca ctctctgcta ataaagtgaa gactgcaaaa
     1741 tggatgtgga atatatatag acaatgctta catttatctt ttactaaatc tttatattgt
     1801 ttttttatgt atgcttttaa tggggttatt aaggagataa tgcatttacc attgaaagcc
     1861 ttaagaaaaa agtaataaaa aggtgaggtt gatgttttga ttttgtttta cactttaccg
     1921 ccacctgcat atatttatat aaaatcaata actagctctt tgattgtatc tctgatgttt
     1981 ttaacttact actttatctg ccattattaa taaaaaagaa gtctttatcg gaaacctaga
     2041 acgaaaaatt agatgttttt tttgttacga taaagaacta ctgtaggtga ttttatttct
     2101 ttaggaatgg gtttttatat tctgattatg ctacaaaaag tgtttctatc agaggaaaat
     2161 aattaaaagt aggttcttat gctaagcgag gtacattata tattcaaggg tgaatatgcc
     2221 ctgattgttt gaaactgttt ttcgcacttt atatataaat aaaaccttct tatataaata
     2281 aggagtgcga aatgggattt gaaaaattgt aggtggagtt tccagagtgg cataggatat
     2341 atggtagtaa ctcttcccta aataactgtt cagaatagta gagcactcag gggggaactc
     2401 agtccggttt atgcgatcct atcaatcgct gaatatccca aatcatcaac tggattgagc
     2461 aatgccggtt atagcattaa ttcccagaac taaacgacgc ctgatttagt aaacgctcca
     2521 taaaacaagg ataaaaacca cgcccggcgg ctcacagcca tgctaatgct gcattgtggt
     2581 gacagcgtca gccatgttgc cagcacagct ttgctgtgtc cgctcatcga ttggccgctg
     2641 gattaactgg ttcacgctgt ctggcactga aggcctgaag tcattgccgt caggacgtgg
     2701 gagacgttgt ccgttcgcac atgcttgcgc attattgctt gagcttgttg agcattctcc
     2761 cggcgatttc ggttatcagc gttccctctg gagttgcgaa ctgctggcga taaaaatccg
     2821 tgaggtcatc ggttgtccgc tacatgctca accatccggc gatggttacc tgctgccgga
     2881 ctggtatggc gcagattagt gccaacactt cgtatccggg acacgaataa ggaagaaaaa
     2941 atggcagcga ttaatgtggc gttagtcagt tgtagcacag aaccctcggc gttttactaa
     3001 gatgaagagg atatccacct caatcccaaa attggcgctg actggcagtt acgtgggcag
     3061 aaatagcaaa ggttcatcat tatttatcag tatgttaaaa catctgaagg cgacatacct
     3121 gcgggcaaaa acgataacac tcattgtcga taactacatc atccacataa gccgtgaaac
     3181 gttgcgctgg ctgaaagcaa acccaaagtt caagggcatt tatcagccag tttactagcc
     3241 gtgggtcaat catgttgctg actttagtca gtttcgggcg catggcggct gaaacagata
     3301 accacctgct atgagggggg ggctgatttt gaacggttgt ggcaagcgtt tcatgaaata
     3361 ataactcaaa atcacctgtg cagttcaatg tggcagctgt tgaacaaggt tcgtcacatt
     3421 atggatgccg tcagctcatt tcccagtgga aagcataggc tggctaaagt gtagcggtat
     3481 taggagcagt tatttagcag aagatataat gaaaagctac cacatcagga gttgacgttc
     3541 actacttttg atctttggat aataaacatc gaatggcaat catatgaaga aaaaattgat
     3601 aattatcgga gctggcgggt ttgcaaaagc tgtaattgat agtttagatc atgcatgtta
     3661 tgaaattcat ggtttcatcg atagttttaa atgcggagaa catcaaggct ataaaataat
     3721 ttcgagaaca atagatagtt tagatgaacc tcataaatat tgctacttta ttgcgattgg
     3781 cgaaccctat tatagggcta tctggttgga aaccattaat gccatgaaac ttgaaactat
     3841 aaatgttatt gacagaacgg caataatttc taaaggcgca attcttgggt catgcatcta
     3901 tgttggtaag atggcaatag taaattgtga ttcgcatctt gaggatggtg ttgtcattaa
     3961 tacaagggct ctcgttgaac atggtaatta tatatcatat tgttccaata tatcgactaa
     4021 cgtcgtcttg aatggtgatg tttttgttgg tagaaaatgt tttataggaa gttgtacagt
     4081 cgttaatgga caactgaaaa taggcgattc gtccatagtc ggctctggtt cagttgttat
     4141 tcgaaatgtt gatgaaaacg ttgtagtcgc tggttctcca actagactta ttaggaagag
     4201 gtaaaagaat gtcatctgta tatatagttg cggaaattgg gtgtaatcat aatggagatt
     4261 ataatttagc taaattgatg atcgatgagg cttatagtgc tggtgtagat gctgttaaat
     4321 ttcagacatt taaagctgat agcttaatat caaaatatgc acctaaagca gagtatcaaa
     4381 ttaaaacgac aggaaatagt gagtcacagt tggaaatgac tcggaagtta gagctttcct
     4441 gtgaggaata tatcaagtta gaagagtatt ctaaaagttt aggtttggat gttttttcaa
     4501 ctccttttga tttggaatct atagaatttt tatacacaag aaaacaaact acatggaaaa
     4561 taccatctgg tgagttgctt aatcttccat atttagaaaa aattgcgtta ttgccaggtg
     4621 ataaaaaaat tattctatcg acaggtatgg caactctcgc cgaaataaag caagcattga
     4681 agatatttac tgataatgga gttaaagccg aggatattac tatcttacat tgtaatacag
     4741 agtatcctac accttatgaa gatgttaatc ttaatgttct tgctggatta aaaaaaatct
     4801 tccaagaata taacattggt ttttctgatc attctcctgg atactttgcc ggtatagcgg
     4861 ctgtacctta tggaataacg tttattgaaa aacattttac tttaaataaa gattttgaag
     4921 gtcctgatca taaagcatcg attactcctg atgagctgag gttattgtgt aatggcataa
     4981 gagcgattga aagagcatta ggtagttttg ataaaagagt tactaactct gagaggaaaa
     5041 ataaactagt ggcaagaaaa tctattgttg ctaagcgtga tataaaaaaa ggggaaattt
     5101 ttactaccga taacattact acaaaacgac ccggaaatgg aattagtcct atgtattggt
     5161 atgaggtttt aggtaaaaaa gcggagcagg attttttaga ggatcaatta ataaaagtat
     5221 ctggttttga agaacaagag atttaaatat gaggaaaaag attgcaatta tcccagctcg
     5281 ttctgggtca aaaggattac ccaataaaaa tattttaatg ctttttgata agccgttaat
     5341 tgcatataca attgaagctg ctatcaaaag tgaattattt gagaaagtta ttgtttcgac
     5401 agactcttgt gaatataagg atattgcttt acgccatggt gctgaagtta ttatgaggga
     5461 caaaagtcta gcttctgata cagcaacatc atacatggtt gtaaaagatg tgttagagaa
     5521 aaatattagc tgtgattatt ttgttttatt acagccaact tcgccattta ggaattataa
     5581 acatataagg gaagctgttg aaaagtttga aaatacagat gacattgatt ttttggtttc
     5641 aatggttaaa acaaataaaa gtgcagattt aattaatcag ttggctccca atgataattt
     5701 aaaatatttc aatgcggact ttgctaatta tagaaggcaa aaccaagaga actattgtcc
     5761 taatggtgcc atatttatag gtaatgtcga tgcatatctt agacagaaac atttttttgg
     5821 tgaaagaagt attgcttatg tgatggatag aattgattca ttagatattg atgacaaact
     5881 tgattttgag tttgcgatat ctctaatgct taaaaaaaat aggatgaaag accttcaaaa
     5941 ggaaatcttg aaaagaataa aggaaaagga attttttttt gataaaataa agccggtgac
     6001 attagttggt cattcaatta tcgattattg gaatgttact catttaaatg gacgtgaagt
     6061 gaataattta ggtattgctg gcatcaatag taaggagtat tatgatctta tcataaaaaa
     6121 taacattatt aaatttggtg atacagtcat acttatgctt ggtacaaatg atattgttgt
     6181 tgatgactgg agtgttgact ttacaattct ttggattgaa aaaatagtta aacatataaa
     6241 aaaaataaat cacaatgcta gtatatatat tttgtcgatc ccgccagtgt tgggacgtat
     6301 tgatagaaac aatgaaacaa taagaaaatt aaactccgaa ttgtcaaagt tttttgaaaa
     6361 ttatcaaaat gttattttta taactctttc aaaagttttt tatgatcaat atggaaatct
     6421 aaaggaaaac tttacatatg atggactgca ctttagtcag ttagcttata atcaattgac
     6481 gaaagagata gaggaaaaaa taaaatgaaa aaaatattat atataacagg ctcaagagca
     6541 gagtatggga taatgaagag gctgttaaga aaacttaagg ataacgaaca tattgaactt
     6601 tcaattattg caaccggaat gcattgtgat gcgaagtatg gctatacata tcagaatatt
     6661 attgacgatg gtttcgaagt taaagagtta tttgatataa atatagagtc atcaacaaac
     6721 agtggtatca tttctacaat gagtcgagct caaaatttgt ttggtgctta ttttgaggaa
     6781 tataaatatg atgcaataat aatacttggc gatagatatg aaatgctttc tgtagctata
     6841 gcggcatcta tacatggtat tccaataatc catttacatg gaggagaaca gacactaggc
     6901 aattatgatg aattcattcg ccattgtata acaaaaatga gtcacttgca tttagttgca
     6961 acagagaaat atagggaaag agtaatacaa ctaggcgagt tgccgtcttg ggtcataaat
     7021 ataggtgcta tgggagccga gaatacagta tctttgtcaa taccagaaca taaagagcta
     7081 caatcaaaat atgaaaattc attaagcaat tatttcatga ttctttttca ccccgaaacc
     7141 ttaactaaaa ataaatctgt tcatgatcaa ataaaagaat taattaaagc attggatatt
     7201 gcacatcaaa aatatcattg taattttata tttatcggta ctaacgcgga tacaaattca
     7261 gatattatta ctaaggaaat tctcacgttt tgtgagtcat ataactttcc ttatttacta
     7321 tcattaccaa ctcaagatta cttaggatta tgtaaatatt cgttaggact tattgggaat
     7381 tcatcttctg gactaataga gatcccatct cttggagtgc ctactattaa tattggttct
     7441 agacaggctg gacgagaacg aggaaagtcc gtgattgatg tggaatgttg ctatacagat
     7501 attcttgaga agatagatga cgttatttca aatcgaatag atagaactta ttataatcct
     7561 tattataaag aaaatagtgc cgagatagct atgtctagta taattagttt cttagattgg
     7621 attagtgata acaaaaatcg cattaaaaag ttttatgata ttcctaaagt cactactcta
     7681 attaattcat aataaccagg cagtcttagt ctatgtaaca ctagttcaca ttactaaaag
     7741 taacagttgt aaaagtagat atactaccta tttactctct aatcttcact atctctaaca
     7801 tatcataaat attaagataa ttatttgtgg tgattaatat acacgattat tgattctgat
     7861 ataaccgaaa atctttgaaa ttacagtccg agaacgaggt agttaagatg acataaacat
     7921 ataagtttct cttgaacccc atctgcgggc tgttcgtata tatttgggga gtcagagcga
     7981 atatgactca ctgtggataa ctatttgtta aaggctggct gttcatcata tgtatctgga
     8041 tacgccagca ttagcgggat attaggcgat gatggggact tactggtgct gaatattagt
     8101 ttcggaaaaa cttgaatgtc aaaggcggaa actgtgctac agataatgat atcttttgcc
     8161 aggcttcttc ctattttacg cacgcggagt ttattgcttc ctgaaaaaat atgggactac
     8221 tgaatacgtt gcgtgagcat tactgaggcg gacctgtatg gagtgagctg tatattgccc
     8281 caggaacaat tactgatgct gccttttggt gctaattaat acttactatg tgttcaataa
     8341 ttgagttatt cctttatcta aaaattttta tcgtcgctta tatctgatca aataccggtt
     8401 atataaataa ggaggacgaa atgaaattta aataatcagt ggaacatatc cgacagttaa
     8461 atatttgtgc cgtcacattt ctggtgcaga agttggcaga tatagaagat tttggttata
     8521 cacatccaag caatatggta gtttgaaaac ggtattccta cgcgctgtac cattgccaga
     8581 gttctatcct atatctctga gaaaaatgta tgaacgatga ccagtcggaa agatactgct
     8641 gcagatcgct gagttattcc tcagttcgtg gcagaatgtt tggagcaact atgtgtggct
     8701 gattttactt atggtacgcc ctgagtttat atgtgcacga aagcccaaat gaactaagag
     8761 tctcttggaa cattggacac aatcattttt cgcgcggata acgactcgga agttatctct
     8821 ctctcactga ctggcgagta gaaaaacatg gagtaaaact ggagtttatc ctacagggta
     8881 agccaatgca taacacttgc tttgagcgct ttaaccggac atatcgtacg gaaatactcg
     8941 atttttatct gttcagcacg ctgaatgaag tgcaggaaat cacggaaaaa aggttgtcag
     9001 aatctaactc ctcatgaata actgcacaat gtgactacgg agaaatattg ataacacatg
     9061 atttggccgg aagttcaaaa cagcatagat ctaaagcagg tatctttaca gaacagcccc
     9121 gcgaatgtag ggatgttggt ataataattt aagctgactg gaaggtctgg gcgagatgac
     9181 actagtagac gataaaaagt ttattgttat tcatttaaaa taggagataa agatttgacg
     9241 gctttagttt agagataaca cctctctagg aaaattgaag aagttctatt ttgctgtaat
     9301 gctttcttat ggatgtagta atgaatgaag acagcagcaa atttatatgt aagtggaaaa
     9361 gttattcaaa agggtatgta tgaaaagtaa tgtttatata tgtaaaacaa catgggaaat
     9421 tttcgtttct attttgttgg ctagcgatat atactacgac aagaaaataa aatctgtttt
     9481 tgttattgaa gtggctgaag agaacattaa ttatctttct aagttatctg attttttctt
     9541 tgtatcaaag gttattcctg ttaggttttc ttctaataaa tatgttaagg ctttatgttt
     9601 tcattataga gttagatatt atttgccaaa aatgctcggc gagtatctta aaagtgatgt
     9661 aagggttaat ctatttatag atcagggagt atttggacaa tattttatta agaataataa
     9721 agatgtatat ctatatgagc atgggaatgg gaattattgt gtaggcccat atcctaatta
     9781 taaaatgata aaaaaatttt tgggtataac tgaaggttat ggtaggcaca gtagagtaaa
     9841 gaggatatat ctacagcatc cagaaaaagc tcctaaggat attattcata aggtgacggg
     9901 tttttctata aagaatttat ataataaatt gttcttgaaa gaacagcaaa acttattgtc
     9961 tttttttggt attcataaaa ttaataaaac gagtgatgtt atcattttaa cacaaccaat
    10021 cagtgaggat ggtttttata gtgaaagaga aaagatcgaa atctataggg agattattga
    10081 tagccgcttt ccttctgata aatctcgcgt agttataaaa ccacatcctc gtgataaaac
    10141 aaattatggt aagtacattc ctgatgtaat gcttttgcca agaagttttc ctatggaaat
    10201 attaaacttc acgaatatta ggtttattaa agcaataaca atctgttcaa gcgctgttta
    10261 taattttgag tatgatattt ctgtggatta tataggaaca ttaagatacc ccaaattaat
    10321 taaagcattg ggaagattgc ctgcacagta tgtgcataag gataactata aaaacattca
    10381 atattaacag ggtgttaata gggagttaat atgatacctt atgttatgtt ttattgttcc
    10441 tctttagttt tagttgttct atctttggta ctgaaaaagt atgattggtt ttggttgttg
    10501 tttcttctat ttttttcagc tgtgtttgtt ggtcttaggg tagatgtcgg tgccgattat
    10561 actgaatatg cccaaattta taatcaatct ggtaatatta caaattttga attgggtttt
    10621 gatataatat ttaactatgg gaaaaggctt ggatatgatt atgtatttgt gtctttattt
    10681 ttctttttgc taactacatt attttttatc tactcaatca aagagttgaa ttataagaca
    10741 cttatttatt tttgtttttt gcttttcatg tttgtccctt taacatcaac aatccgacaa
    10801 ggcttagcta ttcctttttt tgtaatgtgc attttgaact ctgataggcc caaagtctac
    10861 tttacaagta tagccttagg gtgtcttttt cattactcaa ttttatttat gttttttttc
    10921 ttttgggtca gacatattaa gcagtcgtat tgtagggctt ttttaattgt tttgcttttt
    10981 tcattgttga gtatttttaa tatcgttgag tatataatta tgttattaaa ttatatgcct
    11041 ggtttagggg ctataagcac taaacttctt atgtatactc agcgatatca agttgagttt
    11101 tcaatgaatt cttttgttta taaattggct ggtttactat tgattacaat gtttatgcca
    11161 tttataaaag taaacgaaaa attacgttgt agttacaact tgtattttat tacagtatgt
    11221 atatttattt tgttgaaaga caatgctgtt ttaacaaacc gtttgacgta tgctaccaat
    11281 atatcattgg tgactttttt ttcattgtta ttatcgatga tacgtgggtt ctataaaaaa
    11341 gctattgttt ctattttgtt tgtgttttat ttttctttta gttattttaa gttcacagcg
    11401 acagatttaa gacactcagt tgaatctgct tatatgcctt ataaatcctt gcttataaac
    11461 tgaataagac attaagttat gaaattattg aaaagcagat tttcttattt tgccactgaa
    11521 ttaataaata agtgcgtccc attcttttta ttaccatatg tgtcttctgc ggtgggcgta
    11581 gaagtttatg gtaaaatcga gttatttacc actaattata taatattatc tttccttttt
    11641 gccttggggg ttgaggggtg gatgaatgcc cattacttta aaatggctga aagtgatttt
    11701 attactgtac tgtcatcatt ttatctatta agttttctga tttttgttat ttgtatggca
    11761 atatctctac catattcata ttattgttct gcgcttcttt ttgttggtta cgttcaatcg
    11821 cttcttaata ttacaacttt acatttgaga ctaaagggta aatatttaaa tgctggattt
    11881 ttattattgt tatcatcaat tgttaatgct gtgatggtat atgcgagttt tgattggttt
    11941 ggtaagactt atgagtcgcg tattaactct tttttgtttt cttccattat aatacttgtg
    12001 tttattgtgt ttttaagtat acgtgataag aatattaaaa taaaaattga ttttagcttt
    12061 ttaaatagag acattttatt gttttgcctg cctctgtgtt tgactatgat tattaattgg
    12121 gttaaaggta atattgataa atattttatt gctaatctac ttgatttgaa gtctcttggg
    12181 atttatgcgg ttgcttatca gttggcttca gttataaatg ttgtcgggat tattttgaac
    12241 aggaccttac aggctgacct gcttaaaagc atggctgatg gtttttatca aacaaaaaaa
    12301 ataatattca cttttttatt tatattgtgt gtgttttttt ttctgtatat agtagctgtg
    12361 tattgtggct ttagttatgt tttttctatc gactactcct cttctcgcaa tatagcttta
    12421 ctgttgatga ttggtttttt gttattatct ttttcctcat ttctaattaa ttttttatta
    12481 ttctacagga ggccaaggtt gatattgttt caaacctttt taattacgat gatacatgtg
    12541 atgctatctt ttattttgat taaaaaatac gcgctgcttg gagttttact tacacagttc
    12601 gttacttcgt tattaacctt tgtatccact ttattagtat gccacatatt aacgaaatca
    12661 tctcaaaaat gacaggttct taaatcgaga gaagaaggca tttaacataa tctaaaatta
    12721 aagttgcata ttatctctaa taaacaatgt taaaataact gaacatcata gccggtatgg
    12781 caaaaatagg catggttttg ctctcaataa cggaaattat gggtatttcg tctctatttt
    12841 cgactgttta cgtgaaaagg tggaagcagt tattgctgac aatccagcgc agaaatttat
    12901 actgtgaaag ataatcgttt aattattgaa actcctcgtc gcaacctgtt aatggtgaaa
    12961 gtaggtgttg gcacgatact gcattgattc acagaaacca tatctagata atgtcagcgc
    13021 cagtgatata agacgttaat agggatgtgg acaatttcct ggactctcag gagatgaacc
    13081 atgtttactt aagacgaaaa aattcgcacc attgagcttt atttcaatta tggtaacgtg
    13141 ggttgagcta tctttcgaaa agaaatctgc agttttggat cagcaggggg tggcgcaaaa
    13201 gaatccattc gtaatagaca ccgttactca gatgtgcaaa aacaggttgc cgttgaacat
    13261 tatcttaacc acgactgctg tctggcattt accggcagaa ttctgggtta tccctgtacc
    13321 gatgtgctcg ctcgctgggt taatgaacgt taccccgaca gacgacgtat atttaccagt
    13381 aagaccagtc cggatacgtt ctttgaacca gaagtcaaac gtcaagctgt cacggcactc
    13441 tgtacatgac gagtatcaga cagtgaagtt gccagacgtg ttggtgccag ccgtactgta
    13501 ttgtataaat ggtaagatga aattatcggt aacagtgttt accaaatagc ataaacataa
    13561 cgaaccttct ctggaggcag aacgcgatac gttgcgggag gatgtcgccg actgaaacaa
    13621 gaaatacgtc gcagaagata gagctgcata ttctgaaaaa ggcggaggaa atcataaaaa
    13681 aagacccggg catcagtatc agtcacctga acaacaggga aaaaacaaag atcgctgatg
    13741 ccctgagaca aacatatccc ctgacagaat tactgcatgt tctgggcctt gcccgtagca
    13801 gttattttta tcaccgggct gcactgaaag ctggtgataa atacgccacg atacgtacga
    13861 tgctgacaga tatctttaac agtaattacc agtgttatag ctatcgtcgc ctgcatgcga
    13921 tgctcaggca tgaggcggtt tagctatcag aaaaggttgt acgcagactt atggtggagg
    13981 aacagcttgt cgtcagccgt aaccgtcgtc gctacagcac atattgcaga gaaatcggac
    14041 cggctgctga taattttatc gccagagatt ttaaggcgga gcaagctaaa cataaatggc
    14101 tgacagatat cacggagttc cagttcctca cagggaaaat caggctatca ctggtggtgg
    14161 acttcttcga tggaaaagtt gtgagcagtt ctctcagtgc acgtcccgag gctgaactgg
    14221 tcaacaccat agtggataaa gctgtcgaaa cgtaaaatgc aggtgaacga ccggtgatat
    14281 acagtgacaa aggaggcatt atcgctggcc aggctagctg gaaagagtga atgcagcagg
    14341 tgttattccc tcaatgtccc ataaagggtg tccacacgac aatgctgcgt gcgaaggctt
    14401 tttcggcaga ttgaaaacga aaatctatta tgggcgtaaa taatcgggca tcaagcctga
    14461 taaattcagg catcatgtgg atacttacat cagatggtat aacgagcggc gtataaaatt
    14521 attgctggtg cagtcagctc agaaatgtac cgtcaacaat gtggcgggca taataaagca
    14581 gttcaggaaa tcgtcctcat ctaaaaaatt gattgaacat atttctatta acacatcagc
    14641 cgggaaagtg gaacataagt tgtctaaaat tctactgttg acaattttcg ccgtcatttt
    14701 gggtgcagaa ggctgggaag atatagagta ttttggggaa acacctcttg attttttgaa
    14761 gcaatatggt gattttgaaa atggtattac tgttcacgat accattgcca gagtgtatcc
    14821 tgtatcagtc ctgcaaaatt tcacgagtgc tttattaact ggatgcgtga ctaccattct
    14881 tcagatgata aagacgtcat cgcaattgat ggaaaaacac tccggtattc ttatgacaag
    14941 agtcgccgca agggagcgat tcatgttatt agtgtgttct caacaatgca cagtctggtc
    15001 atcggacagg ttaaaacgga taagaaatcc aatgagatta ctgctatccc tgctcttctt
    15061 aatatgctgg ttattaaagg aaaaattatc acatcagata cgatgggttg ccagaaagat
    15121 attgcagaga agatacaaga atagggagat gattatttat tctttgcaaa cagaaatcag
    15181 ggttggccta ataaaaattt tgaggggaaa ttttcactga aagaattaaa tcatccagat
    15241 catgacagtt acgcaattag tgaaaagagt cacggtagag aagaaatcaa tcttcagatt
    15301 gtttgcgaag tccgtgatga acttattgat tttacgtttg aatggaaagg actgaaaaaa
    15361 ttatgtgtag cagttttctt tcggtcaata agtgcaaaac aaaataaaga gccagaaatg
    15421 acggtcagat attatattag ttctgctgat ttaaccgcag agaagttcgc catagcgctc
    15481 cgaaatcact agctcgtgga gaataagttg cactggtgtc tggacgtggt aatgaatgaa
    15541 gacgattgca aaataagaag aggaaacgca gcagaattat tttcagggat acggcacatc
    15601 gctattaata ttttgacgaa tgataaggta ttcaaggcag ggttaagacg taagatgcga
    15661 aaagcaacca tggacagaaa ctacctagcg tcagtccttg cggggaaagg gctttcgtta
    15721 tcttgccctg attatatccc ctctctgaaa gatccacgtg tcgtgaccat ccaaagtgaa
    15781 ggttagtctg caagtaaaac tagggtatga taaagctagg atcaatgagt aagtttgtcg
    15841 tgcgctgtcg tatctgattt taaatatttt ttacacctaa attttttctc agttacgtgc
    15901 tggtttttag gagtgcaact ggatcctgaa ccacggtgaa atcgtaaaga aataattttg
    15961 atttttttat agagactatt acttagttca gttttatttg ataaaattaa ttaattgaaa
    16021 agtagtgaga tgtttttgtg agcggggcta ttttttagat atttgtttac tgtgacaatg
    16081 aactgatttg acacagtgca tgttgctatt agccattgcc tgctcctcac tagcccattt
    16141 agtatcgtga atacaaatga agtagtgggg aatccattaa cacatatatg ccatttatca
    16201 agttgagttg ctatatacat aaaaaaatca cttttactat aagagcgcta taaaatgaaa
    16261 aaaatcactt ttgttattgc tgatattact tttgttggag gtattgaaag agtaaataca
    16321 ttacttgcga ataaattttt tagcgaagga tataatgtag aaatcatatc cctctacaat
    16381 accaataaaa aggttaatta tgagcttaat aattgcatta aagttaattt tgtgaaagat
    16441 aattcctact caggtgcacc aggaagtttt gggcggctaa agcaacacat cagttcacag
    16501 tttgcgttat ttaaggccat taaaaattca aatgctgatt tttatatcgt taattcattt
    16561 ccaatggctt ttttgtcatt tttcaatgtc ttttcaaata aaaaatatgt tgttattgaa
    16621 catgtgcact atgactatta cggggtagcc atcaagtttt ttagaaactt attatataag
    16681 ttttattaca aagtggtttg tgttaatgaa agtgacttga gtaaattttc caaacattta
    16741 aacaatgctg ttaaaatcaa taacccgctg agtttttcat cttcagaaat aacctgtttg
    16801 agcgaaaaga aaattatagc ggtaggccgg cttgagtatc aaaagggatt tgatatatta
    16861 atagatattt ttgcgagagt taataaggaa catcctggct gggaacttca tatatatggc
    16921 gtcggaactt gtgaagagtt tcttagagat aaaataaatc agtataaact aaataatata
    16981 aagttaatgg gctgtgttga taacatacag ttatattatc ctaaatattc tgttttcgta
    17041 ttttcatcaa ggttcgaagg cttcggaatg gttttattag aagctatgga gtgtggtttg
    17101 ccatgtattt catttgattg tcctactggt ccatctgaaa tactagggaa tggacagtat
    17161 ggcatcttgg ttgagaatgg gaatgttact aaatttagtg cggaattagc tgatctaatg
    17221 tccgataaag aaaagaaaat taaactttca aatatatcaa aaactcgtgc aaaagaattt
    17281 catatagaca agatttttag tgaatggaga aaattaatta attagtacgc atagtatgta
    17341 actgaaaata tcgtacaaat gatttttatc agaagattac atgctcaatt aatataatta
    17401 agtaagttac aaatctctga caggagtaaa caatgtcaaa gcaacagatc ggcgtagttg
    17461 gtatggcagt gatggggcgt aaccttgcgc tcaacatcga aagccgtggt tataccgtct
    17521 ctattttcaa ccgttcccgc gaaaagacgg aagaagtgat tgccgaaaat ccaggcaaga
    17581 aactggttcc ttattatacg gtgaaagagt ttgttgaatc tctggaaatg cctcgtcgta
    17641 tcctgttaat ggtgaaagca ggtgcaggca ctgattctgc tattgattcc ctcaagccat
    17701 acctcgataa aggtgacatc atcattgatg gtggtaatac cttcttccag gacaccattc
    17761 gtcgtaaccg tgagctttct gccgaaggct ttaactttat cggtaccggt gtctccggtg
    17821 gtgaagaagg tgcgctgaaa ggtccttcca ttatgcctgg tgggcagaaa gaagcctatg
    17881 aacttgttgc gccgatcctg actaaaattg ccgcagtggc tgaagacggt gagccatgcg
    17941 ttacctatat tggtgccgat ggcgcaggtc actatgtgaa gatggttcat aacggtattg
    18001 aatacggcga tatgcagctg attgctgaag cctattctct gcttaaaggt ggtctgaacc
    18061 tcaccaacga agaacttgcg cagaccttta ccgagtggaa taacggtgaa ctaagcagct
    18121 acctgatcga cattaccaaa gacatattca ctaaaaaaga tgaaaacggt aactacctgg
    18181 ttgatgtgat cctggatgaa gcggctaaca aaggtaccgg taaatggacc agccagagcg
    18241 cgctggatct cggcgaaccg ctgtcgctga ttactgagtc tgtgtttgca cgttatatct
    18301 cttctctgaa aaatcagcgc gttgccgcgt ctaaagttct ctctggtccg caagcgcagc
    18361 cagcgggcga taaagctgag tttatcgaga aagttcgtcg agctctgtat ttgggcaaaa
    18421 tcgtttctta cgctcagggc ttctctcagc tgcgtgcggc gtctgaagag tacaactggg
    18481 agctgaacta cggcgaaatc gcgaagattt tccgtgctgg ctgcattatc cgtgcgcagt
    18541 tcctgcagaa aattaccgat gcctatgccg aaaatccgca gatcactaac ctgctgctgg
    18601 ctccgtactt caagcaaatt gccgatgact atcagcaggc gctgcgcgat gtcgtcgctt
    18661 atgcagtaca gaacggtatc ccggttccta cattcgctgc tgcggttgcc tattatgaca
    18721 gctaccgtgc cgctgttctg cctgcgaact taatccaggc ccagcgtgac ta
//
