LOCUS       BGC0000790             11950 bp    DNA     linear   BCT 01-NOV-2006
DEFINITION  Escherichia coli strain H515b O-antigen biosynthesis gene cluster,
            complete sequence.
ACCESSION   BGC0000790
VERSION     BGC0000790
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
COMMENT     ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2022-11-07 20:35:25
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 11950
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..11950
                     /db_xref="taxon:562"
                     /mol_type="genomic DNA"
                     /organism="Escherichia coli"
                     /serotype="O103:H8"
                     /strain="H515b"
     misc_feature    1..11950
                     /note="O-antigen biosynthesis gene cluster"
     subregion       1..11950
                     /aStool="mibig"
                     /contig_edge="True"
                     /label="Saccharide"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..11950
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            269..1153
                     /gene="rfbB"
     CDS             269..1153
                     /NRPS_PKS="Domain: NAD_binding_4 (7-132). E-value: 3.4e-13.
                     Score: 41.1. Matches aSDomain:
                     nrpspksdomains_rfbB_NAD_binding_4.1"
                     /codon_start=1
                     /gene="rfbB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) NAD_binding_4"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010:NAD-dependent epimerase/dehydratase (Score:
                     224.6; E-value: 3.3e-68)"
                     /gene_kind="biosynthetic-additional"
                     /note="putative dTDP-D-glucose 4,6-dehydratase"
                     /product="RfbB"
                     /protein_id="ABK27313.1"
                     /sec_met_domain="NAD_binding_4 (E-value: 7.5e-14, bitscore:
                     41.1, seeds: 102, tool: rule-based-clusters)"
                     /sec_met_domain="Polysacc_synt_2 (E-value: 6.4e-09,
                     bitscore: 24.9, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="RmlD_sub_bind (E-value: 8.9e-13, bitscore:
                     37.6, seeds: 37, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIVGTYVLLE
                     AARNYWSALDGDKKNSFRFHHISTDEVYGDLPHPDEVNNTEELPLFTETTAYAPSSPYS
                     ASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKGLPIYGKG
                     DQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDLLDEIVPKEKS
                     YREQITYVADRPGHDRRYAIDAEKIGRELGWKPQETFESGIRKTINWYLNNNDWCRRVQ
                     DGSYQRERLGVIR"
     aSDomain        290..664
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_rfbB_NAD_binding_4.1"
                     /evalue="3.40E-13"
                     /label="rfbB_NAD_binding_4.1"
                     /locus_tag="rfbB"
                     /protein_end="132"
                     /protein_start="7"
                     /score="41.1"
                     /tool="antismash"
                     /translation="HQPDAVMHLAAESHVDRSITGPAAFIETNIVGTYVLLEAARNYWS
                     ALDGDKKNSFRFHHISTDEVYGDLPHPDEVNNTEELPLFTETTAYAPSSPYSASKASSD
                     HLVRAWKRTYGLPTIVTNCSN"
     gene            1150..2022
                     /gene="rfbA"
     CDS             1150..2022
                     /codon_start=1
                     /gene="rfbA"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1064:glucose-1-phosphate
                     adenylyl/thymidylyltransferase (Score: 266.5; E-value:
                     4.7e-81)"
                     /gene_kind="biosynthetic-additional"
                     /note="putative dTDP-glucose pyrophosphorylase"
                     /product="RfbA"
                     /protein_id="ABK27314.1"
                     /transl_table=11
                     /translation="MKGIILAGGSGTRLYPITMGVSKQLLPIYDKPMIYYPLSVLMLAG
                     IQDILIITTPEDQQGFMRLLGDGSQFGINLTYAIQPSPDGLAQAFIIGENFIGNDTVCL
                     MLGDNIFFGQGFTPKLRDAAERTSGATVFGYQVMDPERFGVVEFDRNFKALSIEEKPAN
                     PKSNWAVTGLYFYDNNVIDIAKCIKPSKRGELEITAVNEVYLRNNNLTVELLGRGFAWL
                     DTGTHDSLIEAGSFVETVQKRQGMMVACPEEIAWRNGWLNHDELQAKGEFLKKNHYGQY
                     LLRLIKSHE"
     gene            2015..2422
                     /gene="wbtA"
     CDS             2015..2422
                     /codon_start=1
                     /gene="wbtA"
                     /note="putative dTDP-6-deoxy-3,4-keto-hexulose isomerase"
                     /product="WbtA"
                     /protein_id="ABK27315.1"
                     /transl_table=11
                     /translation="MNNNVKLIHFDKKGDNRGFLVVVEQNKDIPIEIKRVYYMFDTGSD
                     VRRGYHAHKKLTQIAIPVKGSCKFLLDDGKSVEHVTLDSPSVGLIIEPMIWHEMYDYSS
                     DCVLMVLADDFYDESDYIRNYADFIREMTNA"
     gene            2415..2948
                     /gene="wbtB"
     CDS             2415..2948
                     /codon_start=1
                     /gene="wbtB"
                     /note="putative butyryltransferase"
                     /product="WbtB"
                     /protein_id="ABK27316.1"
                     /transl_table=11
                     /translation="MRNLSLFTKNTKLIPVEINDAEFIYSLRVDSRLNKHISTINGTVI
                     EQAEWIEDYKKREENNEEFYFIIKRTDNDEKIGTIRLYNITKDDGFCWGSWILNDDKMK
                     SSAIESAYLIYKFGFLHKNYKYSYFQVDKKNKEVIAFHKKTGATIIKEDDVNFHFEFYV
                     ENFIRLREKYSKYF"
     gene            2960..4066
                     /gene="wbtC"
     CDS             2960..4066
                     /NRPS_PKS="Domain: Aminotran_5 (34-167). E-value: 7.1e-07.
                     Score: 20.2. Matches aSDomain:
                     nrpspksdomains_wbtC_Aminotran_5.1"
                     /codon_start=1
                     /gene="wbtC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DegT_DnrJ_EryC1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1056:DegT/DnrJ/EryC1/StrS aminotransferase (Score:
                     324.2; E-value: 1.9e-98)"
                     /gene_kind="biosynthetic-additional"
                     /note="putative aminotransferase"
                     /product="WbtC"
                     /protein_id="ABK27317.1"
                     /sec_met_domain="DegT_DnrJ_EryC1 (E-value: 2.3e-113,
                     bitscore: 368.7, seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIDFLNLKRINAQYEMELKDACSRVIDSGWYIMGTELKTFEEKFA
                     NWCGVKHVIGVANGLDALILVLRAWKEMGKLNDGDEIIVPANTYIASVLAITENNLVPV
                     LTEPDPLTFNLSAGGIRKVITGKTKAILPVHLYGQISPMAEIMEIAAEFGLLVLEDCAQ
                     AHGSSIDGRKAGSWGHAAGFSFYPGKNLGALGDAGAITTSDDQLADILTAIRNYGSHEK
                     YKNKYQGLNSRLDEMQAAFLNVKLKYLDRENKLRRAIAKKYIEEIKNPLISLPVIAGNE
                     MSHVWHLFVIRTSERDSLQKHLKTEGITTLVHYPIAPHNQEAYKEYSFLSLPLTERIHR
                     EVLSLPMDPTMSIDDINMVIEAINRWKS"
     aSDomain        3062..3460
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_wbtC_Aminotran_5.1"
                     /evalue="7.10E-07"
                     /label="wbtC_Aminotran_5.1"
                     /locus_tag="wbtC"
                     /protein_end="167"
                     /protein_start="34"
                     /score="20.2"
                     /tool="antismash"
                     /translation="TELKTFEEKFANWCGVKHVIGVANGLDALILVLRAWKEMGKLNDG
                     DEIIVPANTYIASVLAITENNLVPVLTEPDPLTFNLSAGGIRKVITGKTKAILPVHLYG
                     QISPMAEIMEIAAEFGLLVLEDCAQAHGS"
     gene            4054..5319
                     /gene="wzx"
     CDS             4054..5319
                     /codon_start=1
                     /gene="wzx"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt"
                     /gene_kind="biosynthetic-additional"
                     /note="putative O-antigen flippase"
                     /product="Wzx"
                     /protein_id="ABK27318.1"
                     /sec_met_domain="Polysacc_synt (E-value: 8.2e-15, bitscore:
                     44.6, seeds: 31, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEKLNFAKVTILSGVVTCFRLICGLLISKVVAIYTGPIGIANLGQ
                     LQNFVAFINGFISSQVSQGVNRYSAENQGDYENACFYWRASIKLSFIACCFIISTGVLF
                     SSKISEYLFYQSDLYWLIIIALCALPLNIVNNIFLGVLNGLNEYNRFFLANVLAISSSL
                     LSMVGLVYFFGLKGALVSASLNNAVAGVWLITIIIKRPWFKFKYWVGHTPRHNITQMKN
                     YFYMGVIGALTGPISMIVVRTILTNNFSLEDAGYWQAVNRISEAYLAVLTTALTVYYFP
                     KTAAARRYSEYITLLKTGACIVVPLALSMALTIYGLKDFIISILFTADFIRARELFLFQ
                     NIGDFLRICSWLFATILLAKGYFKINAILEVMFSIIFPVLVKFLIINFGLNAVSIAYSI
                     TYALYFIVVCIIYCWHLTKKAL"
     gene            5323..6309
                     /gene="wbtD"
     CDS             5323..6309
                     /codon_start=1
                     /gene="wbtD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /note="putative glycosyltransferase"
                     /product="WbtD"
                     /protein_id="ABK27319.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 1.9e-29,
                     bitscore: 92.3, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIISSDNLSVIIPVYNEEKNVINILQSLECQSLTGFNVIVIDDGS
                     KDMTASLVKEYKPSSYSLSLIQQSNMGAARARENAINFTHSEYIAFIDSDDSLSSDALE
                     KALAPMLDNKDIDISLFELVHIKNLNHDTNNIFVPYSTTKLICGEEAFANCISYWGLHG
                     FGIYKRKLIQKSYGIYYKYNKNKENYINNDEVISRISFGLSKNIYLSSGRYFFVQNMDS
                     TTRRINESYYKVINNAVYLREYIDEEIKYNDFDCLGEANKLLVSTIWGVFVRYQKWKKK
                     FSDETNGKWREAIKKGMQYIKKINNNKNINLHIKSKMQLYIISKLVS"
     gene            6312..7202
                     /gene="wbtE"
     CDS             6312..7202
                     /codon_start=1
                     /gene="wbtE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /note="putative glycosyltransferase"
                     /product="WbtE"
                     /protein_id="ABK27320.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 5.5e-33,
                     bitscore: 103.9, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKNLLITIIVASYNSKDYILETLESCINQQYQNIEIIIVDDCSSD
                     NSVELIKQWCKEKKSTSPKIRCILVESERNKGIPANLNNALPFINGDWIKCIGSDDILL
                     PEAISEFVNRLEKCSNLDNIGAVFTYFQTFGYSVNVSTRYPSSWTRTICQMPPSWLKKA
                     AAMIHFNNVAPCAFINKNHFSGFDTSYKLLEDLPLWLKMIKNNKNTLFFDYTTVLYRLH
                     DSQITSLNNIKVNDVLINDLCRLNYYRKNNGYYLAYLHHKFNLYCAVKRMRFYRYLKII
                     NPLNLLIVLYERVKK"
     gene            7202..8350
                     /gene="wzy"
     CDS             7202..8350
                     /codon_start=1
                     /gene="wzy"
                     /note="putative O-antigen polymerase"
                     /product="Wzy"
                     /protein_id="ABK27321.1"
                     /transl_table=11
                     /translation="MYILSMAIPLFFISLCWHRFSHQTQVAFRLLFIVFFSLSFFSYYT
                     NGVDWSVYYLRFIEDEGLFTSFEFGFVIILKLLLIVSDDNFGLAILLYYFLCFVLLSLI
                     LKKYKVNEPLFLGCLLLLFGYNLFLEQLRQLLACIIVFYAMLLYNQNKNLIELCILVIT
                     ASTFHVSAVIILPTIFLISFRNVTTFIIITVSSITSIVVFMIALYSAISSLAAMSFVFA
                     KIDYYLHQYPVVLNFGWLNILDALFILFYMRYRNAIDRQININILTRIIFVGSVIHLFS
                     GSITFLARVCFYFYFVGVYIYCYSLNDKTPRVFAIKNFNTLVLAAFVNVVLLLNFASYF
                     RNEQAPVGFYNMNFQFFNTFNDNYLRTNAYDKYFKGMQGSVD"
     gene            8353..9444
                     /gene="wbtF"
     CDS             8353..9444
                     /codon_start=1
                     /gene="wbtF"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1045:glycosyl transferase group 1 (Score: 151.9;
                     E-value: 4.7e-46)"
                     /gene_kind="biosynthetic-additional"
                     /note="putative glycosyltransferase"
                     /product="WbtF"
                     /protein_id="ABK27322.1"
                     /sec_met_domain="Glycos_transf_1 (E-value: 3.7e-40,
                     bitscore: 127.0, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSKRIIFVITGLGLGGAEKQICLLADKLYLHNNEVNIVVLNGNVN
                     VTPLNENIRIHNLHMKKNPLGLLNAVLRLVKIISYFKPDIVHGHMFHANIVARLAKVFT
                     YNKYKLICTAHSKNEGGHIRMLVYRLTDFLCDATTNVSNEALDVFIKKKAFSKSKSIPV
                     YNGIDTDLFKYDPLQRDKIRHSLNIINDDSLILSVGRLTDAKDYPNLLKALLELPERYK
                     LVIIGEGEARNEVERTILNYGLEKRVKLLGSIANVATYYSACDIYVSSSKWEGFGLVVA
                     EAMSCQRLVVATNAGGVAEVVGDMNYIVPVSDPQLLANKINEVMEFDHETKMEVMYRNR
                     EFVLKMFSIDMIVNQWMKLYSSI"
     gene            9630..10751
                     /gene="rfpB"
     CDS             9630..10751
                     /codon_start=1
                     /gene="rfpB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1045:glycosyl transferase group 1 (Score: 120.1;
                     E-value: 2.2e-36)"
                     /gene_kind="biosynthetic-additional"
                     /note="putative galactosyltransferase"
                     /product="RfpB"
                     /protein_id="ABK27323.1"
                     /sec_met_domain="Glycos_transf_1 (E-value: 2.8e-31,
                     bitscore: 98.2, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKVLLIGNQSSTIILFRKAVIENLISKNIKVYTLTMDNNEGNFNK
                     IKKIGAIPLSYSFSRSGINPISDFFNTFFLARKIKEINPDSVLCFFPKPVIYGTLAAMI
                     AGVKKRYALLEGLGFCFTQNKDKDSFKKIVLRKIQGFLYKMTLPFATKVLFLNNDDYHD
                     LVVKNNIKIKDYKVIGGIGVNLNEFSYHPPICGEIHFGMVARLLREKGVCEFVEAAKII
                     KKKYPMVRFSIAGDFDDNPGGIDAAQIVNWKAEGYVEFLGPCSYIKEYLSDISVFVLPS
                     YREGVPKSTQEAMAIGRAIITTDVPGCRETVIDGYNGYLVPPWDTQKLALVMETFIHER
                     ELIVTMGLNSRRFALSKFNESESANSLVNIITA"
     gene            10830..11858
                     /gene="galE"
     CDS             10830..11858
                     /NRPS_PKS="Domain: NAD_binding_4 (4-182). E-value: 1.9e-11.
                     Score: 35.4. Matches aSDomain:
                     nrpspksdomains_galE_NAD_binding_4.1"
                     /codon_start=1
                     /gene="galE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010:NAD-dependent epimerase/dehydratase (Score:
                     232.2; E-value: 1.6e-70)"
                     /gene_kind="biosynthetic-additional"
                     /note="putative UDP-galactose-4-epimerase"
                     /product="GalE"
                     /protein_id="ABK27324.1"
                     /sec_met_domain="Polysacc_synt_2 (E-value: 3.8e-18,
                     bitscore: 55.2, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="RmlD_sub_bind (E-value: 1.4e-10, bitscore:
                     30.3, seeds: 37, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAILVTGGAGYIGSHTVLMLLKEQYEVIVLDNFHNSSVESLHRVK
                     EITGSDICIINGDIRDRVILKSIFTENSITDVIHFAGLKSVSDSIQFPTEYYDNNVYGT
                     LVLIEEMISNNIKNLIFSSSATVYGTPEKIPVKETFPVGGTTNPYGTSKLMVEKILHDV
                     SLAYPFFRTTILRYFNPVGAHPSGKIGEDPNGIPNNLMPYICSVASGKYKQLSIYGNDY
                     ETKDGTGVRDFIHVMDLANGHIAALKHRDEGSNYKVYNLGTGHGYSVLELLAAFQRITS
                     VNIPYVLVERRAGDIAECWSDPSKAYLELGWKACLGLDEMVRDAWNWQQSNPDGYKFSE
                     KN"
     aSDomain        10842..11375
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_galE_NAD_binding_4.1"
                     /evalue="1.90E-11"
                     /label="galE_NAD_binding_4.1"
                     /locus_tag="galE"
                     /protein_end="182"
                     /protein_start="4"
                     /score="35.4"
                     /tool="antismash"
                     /translation="VTGGAGYIGSHTVLMLLKEQYEVIVLDNFHNSSVESLHRVKEITG
                     SDICIINGDIRDRVILKSIFTENSITDVIHFAGLKSVSDSIQFPTEYYDNNVYGTLVLI
                     EEMISNNIKNLIFSSSATVYGTPEKIPVKETFPVGGTTNPYGTSKLMVEKILHDVSLAY
                     PFFRTTILRYFNPVG"
ORIGIN
        1 gcccttaata cctctattaa tcaaactgag agccgcttat ttcacagcat gctctgaagt
       61 aatatggaat aataaagtga agatacttgt tactggtggc gcaggattta ttggttctgc
      121 tgtagttcgt cacattataa ataatacgca ggatagtgtt gttaatgtcg ataaattgac
      181 gtacgccgga aacctggagt cacttgctga tgtttctgat tctgaacgct atgtttttga
      241 acatgcggat atttgcgatt ctgttgcaat ggcgcggatt tttgctcagc atcagccgga
      301 tgcagtgatg cacctggctg ctgaaagcca tgtggatcgt tcaattacag gccctgcggc
      361 atttattgaa accaatattg ttggtactta tgtccttttg gaagcggctc gcaattactg
      421 gtctgctctt gatggcgaca agaaaaatag cttccgtttt catcatattt ctactgacga
      481 agtctatggc gatctgcctc atcctgacga agtaaataat acagaagaat tacccctctt
      541 tactgagacg acagcttacg cgccaagcag tccttattcc gcatcaaaag catccagcga
      601 tcatttagtc cgtgcttgga aacgtaccta tggtttaccg actattgtga ctaactgttc
      661 gaataactac ggtccgtatc atttcccgga aaaattgatt cccttggtta ttctcaatgc
      721 tctggaaggt aaaggattac ctatttatgg taaaggggat caaattcgcg actggctgta
      781 tgttgaagat catgcgcgtg cgttatatac cgtcgtaacc gaaggtaaag cgggtgaaac
      841 ttataacatt ggtggacaca acgaaaagaa aaacatcgat gtagtgctca ctatttgtga
      901 tttgttggat gagattgtcc cgaaagagaa atcttaccgt gagcaaatta cttatgttgc
      961 cgatcgcccg ggacacgatc gccgttatgc gattgatgct gagaagattg gtcgcgaatt
     1021 gggatggaaa ccacaggaaa cgtttgagag cgggattcgg aagacaatta actggtattt
     1081 gaataataat gattggtgcc gacgagttca agatggctct tatcaacgtg agcgtttggg
     1141 ggtgattaga tgaaaggaat tattttagcc ggtggttctg ggactaggct ttatccaatt
     1201 acaatgggag tttcaaagca gctattaccc atttatgata aaccgatgat ttattatcct
     1261 ttgtcggtgc tgatgttggc aggtatacaa gatattctta ttattacaac gccagaagat
     1321 cagcaagggt tcatgagatt acttggggat ggaagccaat ttggtattaa tctgacatat
     1381 gcaattcaac ctagtccaga tggtttagcc caagcattta ttattggtga aaactttatt
     1441 ggaaatgaca cagtatgtct tatgcttgga gataacattt tttttggaca aggttttaca
     1501 cccaaactcc gggatgcagc agaaagaaca tcaggtgcca cagtctttgg atatcaagta
     1561 atggatcctg agagatttgg agtagtggag tttgatagaa attttaaagc tttaagtatt
     1621 gaagaaaaac ctgccaatcc aaaatcaaat tgggctgtca caggattata tttttatgac
     1681 aataacgtta tcgatattgc aaagtgtatt aaaccttcta aacgtggtga attagaaatt
     1741 acggcagtta atgaagttta tctcagaaat aataatttaa ctgttgaatt actaggacgt
     1801 ggttttgcat ggctggatac aggaacccat gacagcttga ttgaagctgg gagttttgta
     1861 gagacagttc aaaaaaggca agggatgatg gttgcttgtc cggaggagat tgcatggcga
     1921 aatggctggt taaaccatga tgaattacag gcaaaaggtg aatttttgaa aaaaaatcac
     1981 tatggacagt atttactaag gttaattaaa tcgcatgaat aataacgtaa aacttataca
     2041 ctttgataag aaaggtgaca accgaggctt tcttgttgtt gttgagcaaa ataaagatat
     2101 cccaattgaa attaaacgag tttactacat gtttgatact ggttctgatg taagacgggg
     2161 atatcacgca cataaaaaac ttacacaaat agcaatccct gttaagggat cttgcaagtt
     2221 tttgcttgat gacggtaaat cggtggaaca tgttacatta gattcaccgt cagtgggatt
     2281 aattatagaa ccaatgattt ggcatgaaat gtacgattat tcatcagatt gtgttttaat
     2341 ggtgttggca gatgactttt atgatgaaag tgattacatt cgtaattatg ctgatttcat
     2401 ccgggagatg actaatgcgt aatttgtcgc tctttacaaa aaatacaaaa ttaattccag
     2461 ttgaaataaa tgatgcggaa tttatttatt cactgcgggt agattctcgt ctcaataagc
     2521 atatttctac aataaatggg acggtaattg aacaggccga atggattgaa gattataaaa
     2581 aaagagaaga aaataatgaa gagttttatt ttataataaa aagaactgat aatgatgaaa
     2641 aaattggcac gataagactt tataacatca cgaaagatga tggtttttgt tgggggagtt
     2701 ggattctaaa tgatgacaaa atgaaaagct ctgcaataga aagtgcctac ctgatataca
     2761 aatttggttt tttacataaa aactataagt attcatattt tcaagttgat aaaaaaaata
     2821 aagaagtaat cgcatttcat aaaaaaacag gagccaccat aataaaagag gatgacgtga
     2881 attttcactt tgaattttat gtggaaaatt ttattcgttt aagagaaaaa tatagtaaat
     2941 atttttgagg ggaaagaaaa tgattgattt tttgaattta aaaagaataa atgctcaata
     3001 tgaaatggaa ttaaaagatg cttgctccag agttattgat tctggttggt atattatggg
     3061 gacggagtta aagacatttg aggaaaaatt tgcaaattgg tgtggagtta agcatgttat
     3121 tggtgttgcc aatggcttag atgcactaat ccttgttctt cgggcttgga aagaaatggg
     3181 gaaattaaat gacggagatg aaattattgt acctgccaat acatatattg catctgtttt
     3241 ggcaataact gaaaataatc tagtccctgt attgactgaa ccagatcctt tgacattcaa
     3301 cttaagtgcg ggtgggattc gtaaagtaat tacagggaaa acaaaagcaa ttttgccagt
     3361 gcatttgtat ggacaaatat ctccaatggc agaaataatg gagattgcgg cagagtttgg
     3421 tttattggtt ttagaggatt gcgctcaagc tcatggttcc tctattgatg gacgcaaagc
     3481 tggcagttgg ggtcatgcag ctggattcag tttctatcca ggaaaaaatc ttggcgcttt
     3541 aggtgacgca ggtgcgataa caacgagtga tgatcaactt gcagatattt tgacagctat
     3601 tagaaattat ggttctcatg aaaaatataa aaacaaatat caaggcttaa atagtcgatt
     3661 ggatgaaatg caggcggcat ttttgaatgt caagttaaaa tatcttgata gggaaaataa
     3721 attaagacgt gcaatagcta aaaaatatat tgaagaaatc aagaatccac taatttcact
     3781 gcctgtaatt gctggcaatg aaatgtctca tgtatggcat ctttttgtta tcagaacttc
     3841 agagcgtgat tctttgcaaa aacatttgaa aactgaaggt attactacat tagtgcatta
     3901 cccaatagcg cctcataatc aagaagcata taaagaatat agttttttat ctctcccttt
     3961 aactgagaga atacatagag aagtcttatc gctgccaatg gatccgacaa tgagcatcga
     4021 cgatattaat atggtaattg aagcgattaa tagatggaaa agctaaattt cgcaaaagtt
     4081 acgatactca gtggtgtagt aacatgcttt agactaattt gtggccttct tatttcaaaa
     4141 gttgttgcaa tttatactgg tccaattgga atagcaaatt tgggacaatt gcaaaatttt
     4201 gtggccttta taaatggatt catttcatca caagtttcac aaggagttaa tagatattca
     4261 gcagaaaatc aaggtgatta cgaaaatgca tgtttttatt ggcgtgcatc aattaaatta
     4321 tccttcatag cctgttgttt tattataagt acgggggtgc ttttttccag taaaatatca
     4381 gaatacttat tttatcaaag tgatttgtat tggttaatta taattgctct atgcgctctt
     4441 cctcttaata tagtaaacaa tatattttta ggggttttaa atggactaaa tgaatataat
     4501 agatttttcc ttgcgaatgt tttagccata tcctcatcgt tgttatctat ggtgggctta
     4561 gtttattttt ttggcctcaa aggcgcatta gtgtctgcat cattaaataa tgcggttgca
     4621 ggtgtctggc ttattactat tataattaaa agaccatggt ttaaatttaa atactgggtg
     4681 ggacatactc ctcggcataa catcactcag atgaaaaatt acttttatat gggggtgatt
     4741 ggagcgttaa ctggacctat ttcgatgata gttgttcgaa ctattctcac taataatttt
     4801 tctttagagg atgccggata ttggcaagct gtaaatagaa tttctgaggc ttatctggct
     4861 gttcttacta cggcattgac agtttattat tttccaaaaa ctgcagccgc aagaagatat
     4921 agcgaatata taactctttt aaagaccggg gcttgtattg ttgtaccgct cgcgttgagc
     4981 atggctttaa ccatctacgg cttgaaagat tttataatta gtatcttgtt cacggctgac
     5041 tttatacgtg ctcgggagct ttttttattt caaaatatcg gggattttct gcggatttgt
     5101 agttggctgt ttgccactat attgttagca aagggatatt ttaaaataaa tgctatattg
     5161 gaggttatgt tttcaataat ttttccagta ttggtaaagt ttttgataat taattttgga
     5221 ctaaatgccg ttagtattgc ctacagcatc acttatgcat tgtattttat agtggtgtgt
     5281 attatttatt gttggcatct gacaaaaaag gccctataac aaatgataat atcttcagat
     5341 aacctaagtg ttattatccc agtttataat gaagagaaga atgttattaa tattcttcaa
     5401 tctcttgaat gccagtcttt aacgggattt aatgttattg tcattgatga tggttcaaaa
     5461 gatatgactg cttctcttgt aaaagaatat aaaccatcat catatagttt aagcttaata
     5521 caacaaagta atatgggggc tgctcgggca agggagaatg ccatcaattt tactcatagt
     5581 gagtatattg catttataga tagtgatgac tctttaagtt cagatgctct tgaaaaagca
     5641 ttagctccga tgctggataa taaagatatc gacatctcac tatttgaact ggtacatatt
     5701 aaaaacttaa atcatgatac taataacata tttgtacctt attctacaac caaattaata
     5761 tgtggagaag aggcttttgc taattgtatt tcatattggg gattgcatgg gtttggtatc
     5821 tacaaaagaa aattgattca aaaatcctat ggtatttatt ataaatataa taaaaacaaa
     5881 gaaaattata taaataatga tgaggttatt tcaagaatca gttttggatt atctaaaaat
     5941 atctatttat catctggaag atatttcttt gtacagaaca tggattcaac aacaagaaga
     6001 ataaatgaaa gttattataa agttataaat aatgctgttt atttaagaga gtatatagat
     6061 gaagaaataa aatataatga ttttgattgt ctaggtgaag ctaataaact ccttgtaagt
     6121 actatatggg gagtgtttgt tcgttatcaa aaatggaaga aaaaattcag tgatgaaaca
     6181 aacggtaaat ggcgcgaagc aataaaaaaa ggaatgcaat atataaaaaa aatcaataac
     6241 aataagaata ttaacctgca tattaaatct aaaatgcaat tatatataat atctaaactt
     6301 gttagctaaa tatgaaaaat ctattaatta caattattgt tgcatcatat aactcaaaag
     6361 attacatact agagacatta gagtcatgca ttaaccaaca atatcaaaat attgagatta
     6421 ttatagtaga tgattgctct agtgataata gtgtagaact tataaaacaa tggtgtaaag
     6481 aaaagaaaag cacatctcct aaaattagat gtatattagt cgaaagcgaa agaaataaag
     6541 gaattcctgc aaaccttaat aatgctcttc cctttattaa tggagactgg attaaatgta
     6601 ttggttcaga tgatattctt cttcccgaag caatctctga gttcgtaaat agattagaaa
     6661 aatgctcaaa tttggataat ataggtgcag tgtttactta tttccaaacc tttggatatt
     6721 ctgtaaatgt tagtactcga tatccatcct catggacaag aactatatgc caaatgccac
     6781 catcatggtt aaagaaggca gcggctatga ttcactttaa taatgtggca ccttgtgctt
     6841 ttataaataa aaatcatttt agtggatttg atacttcata taaactttta gaagatcttc
     6901 ctctatggct aaaaatgatt aaaaataata aaaacacgct attttttgat tatacaactg
     6961 tattatatcg tctacatgat tctcaaatca cctcgcttaa taacattaaa gtcaatgatg
     7021 ttcttattaa tgatctttgt cgtttaaatt attatcgaaa aaataatggt tattatttgg
     7081 cttaccttca tcataaattc aacctttact gcgcagtaaa aaggatgcga ttttacagat
     7141 acttaaaaat aataaatcct ttgaatttat taattgtact atatgaaaga gtaaaaaaat
     7201 aatgtatata ttatcaatgg ctataccatt atttttcata tcgttgtgtt ggcatcgatt
     7261 tagtcatcaa actcaagttg catttcgttt attattcata gtatttttca gtctaagttt
     7321 cttttcatat tatacaaatg gcgtggattg gagtgtttat tatctaagat tcatcgagga
     7381 tgaggggtta tttacctcgt ttgaatttgg gtttgttatt attttaaagc tattgcttat
     7441 tgtttcagat gataattttg gcttggcaat tttactctat tattttctct gttttgtatt
     7501 gctctcatta atattgaaaa aatacaaagt taatgaacca ttatttttgg gatgtctttt
     7561 attgttattt ggctacaacc tttttttaga gcagttacgt caattactgg catgtataat
     7621 agtattttat gcgatgctat tatacaatca aaataaaaat cttatagaat tatgtatatt
     7681 ggttataaca gcatcaactt ttcatgtgtc tgcagtaata attttaccga caatcttttt
     7741 aatctctttt agaaatgtga ctacttttat cataattacg gtatcctcaa ttacaagtat
     7801 tgtggtattt atgatagcac tttattcagc tatatcctct cttgctgcta tgagctttgt
     7861 ttttgctaaa attgattatt atttgcatca atatccagtt gtcctaaatt ttggttggct
     7921 aaatatactt gatgctttat ttattttatt ctacatgcgt tatagaaatg caattgatag
     7981 acagataaat ataaatatat taacgcgaat tattttcgtt ggttctgtta ttcatctgtt
     8041 cagtggatcg attacttttc tggctcgcgt gtgtttttat ttttactttg taggtgttta
     8101 tatatactgc tattcattaa atgacaagac cccgcgggta tttgctatta aaaatttcaa
     8161 tacgttagta ttggctgcct ttgttaatgt agtactttta ttgaactttg catcctattt
     8221 tagaaatgag caagcccccg ttgggtttta taatatgaac tttcaatttt ttaacacatt
     8281 taatgataat tatcttagaa cgaacgcata cgataaatat tttaaaggaa tgcagggcag
     8341 cgtggattaa ctatgagtaa aagaattatt tttgttatta ctgggctggg attaggtggg
     8401 gctgaaaaac aaatatgttt gttagctgat aaattgtatt tgcataataa tgaagttaat
     8461 attgtcgtgt tgaatggaaa tgtcaatgtt actccattga atgagaatat tcgaattcat
     8521 aatttgcata tgaaaaaaaa tccacttggg ttactaaatg cagtgctcag gctcgtgaaa
     8581 ataattagtt atttcaagcc ggacattgtt catggacata tgttccatgc aaatatcgtt
     8641 gccagacttg caaaagtttt tacctataat aaatacaaat taatttgtac agcacatagt
     8701 aaaaatgaag gtgggcatat taggatgctc gtttatagat taactgattt tttatgtgat
     8761 gctacgacta atgtaagtaa tgaagctctt gatgttttta ttaagaaaaa agcctttagc
     8821 aaatcgaagt caattcccgt ttataatggc attgacactg atctatttaa atatgatcct
     8881 ttacaaagag ataaaataag gcatagttta aatatcatta atgatgatag ccttatactt
     8941 tctgtaggcc gacttactga tgcaaaagat tatccaaact tattaaaagc attgttagaa
     9001 ttacctgaac gatataaatt agtcataata ggtgaaggtg aagccagaaa tgaagtcgag
     9061 cgtacgattt taaattacgg ccttgaaaaa agagtgaaat tgctaggtag tattgctaat
     9121 gtcgccactt attattctgc atgtgatatt tatgtatcgt cctctaagtg ggaaggcttt
     9181 ggtttggttg tagctgaggc tatgtcttgc cagcgattgg tcgttgcaac taatgccggt
     9241 ggggtggctg aagttgttgg tgatatgaac tatattgtac ctgtttcaga ccctcagttg
     9301 ttggcaaata aaattaatga agttatggag tttgatcacg aaactaaaat ggaagtaatg
     9361 tatagaaata gagagtttgt tttaaaaatg ttctcaatag atatgatcgt taatcaatgg
     9421 atgaaattgt attcttcaat ctaatccata gcttaatgga aaaatataga atttgataaa
     9481 ttaatacact ctcatatgtg tattatccgg tgcgttatct atagttaaaa ataaaattcg
     9541 gtgttttttg tttcattgtt tttgaataaa tcataattta agttatgatt taaaaaataa
     9601 gatactgtta tattgtaagg tttttaaata tgaaagtgct tttaataggt aaccaatcta
     9661 gtactattat tttgttcaga aaggctgtta ttgaaaattt gatatcaaaa aatattaaag
     9721 tatatacact tacgatggat aataatgagg gtaattttaa taaaattaaa aaaataggtg
     9781 caatcccttt atcctattct ttttcacgat ctggtattaa tcctatatct gattttttta
     9841 atactttttt tttggcaagg aaaataaaag agattaatcc tgattctgtt ctttgctttt
     9901 tcccaaaacc agtcatatat ggtactcttg ccgctatgat tgctggcgta aaaaaacgtt
     9961 acgctttatt ggaagggttg ggtttttgtt tcacacaaaa taaagataaa gactcgttta
    10021 aaaaaatagt cttaagaaag attcaaggtt ttttatataa gatgacatta ccttttgcaa
    10081 ccaaagttct ttttctcaat aatgatgact atcatgactt ggttgttaaa aacaatatta
    10141 aaatcaaaga ttataaagtt attggtggca taggtgtaaa tctcaatgaa tttagttatc
    10201 atccaccaat ttgtggggag atccattttg gtatggtggc aagattattg agagagaagg
    10261 gcgtatgtga atttgttgaa gcggctaaaa taataaagaa aaaatatcca atggtccgat
    10321 ttagtatagc tggtgatttt gatgataatc cgggtggaat tgatgctgca caaattgtaa
    10381 attggaaagc ggaaggttat gttgaatttt taggaccgtg ctcttatata aaagaatatt
    10441 tgagtgatat cagcgtcttt gtattacctt cttatcgaga aggtgtcccc aaaagtactc
    10501 aggaagcaat ggcaataggt cgtgcgatta ttacgaccga tgtgccaggt tgtcgagaaa
    10561 cagtaattga tggatataat ggatatcttg tacctccatg ggatacacaa aaacttgctt
    10621 tagttatgga aacatttatt catgaacgtg agcttattgt cactatggga ttaaatagtc
    10681 gaaggttcgc attgtcaaaa tttaatgaaa gtgaatctgc aaattcttta gttaatatta
    10741 taactgcata agagttatta aattatccaa gctttaataa cattctacct gctagtcttg
    10801 ttgctttgat taaatgaggg tgctgttgta tggctatatt agtgacgggt ggtgctggat
    10861 atattggctc acatactgta ttaatgctgc tgaaagaaca atatgaagta atagtgcttg
    10921 ataattttca taattcttca gttgaatctt tgcatcgcgt taaagaaatt actggtagtg
    10981 atatctgtat cattaatggt gatattcgag atcgtgttat tttaaaatcc atctttactg
    11041 aaaacagcat aacagatgtt attcattttg ctggcctgaa atcagtaagc gattcaattc
    11101 aattcccaac agagtactac gataataatg tatacggtac acttgttctt attgaggaaa
    11161 tgattagtaa taatatcaag aatctaatat tcagctcctc agccactgta tatggtactc
    11221 cagagaagat acccgtaaaa gaaacattcc ctgtaggtgg cactactaat ccctatggga
    11281 cttcaaaact tatggttgag aaaatattac atgatgtttc tttggcatat ccttttttta
    11341 gaacaacaat tttgcgttat tttaatcctg ttggcgcaca tccttccgga aaaattggag
    11401 aagaccctaa cggtattcca aataatttga tgccttatat atgttcagtt gcaagtggta
    11461 aatataagca attatccatc tatggtaatg attatgaaac taaagatggt actggcgtta
    11521 gagattttat tcatgttatg gatctcgcaa acggtcatat agctgccttg aagcaccgag
    11581 atgaaggtag caattataag gtttacaatt taggcacagg gcatggttat tcggttcttg
    11641 aacttttggc tgcttttcaa cggataacct ctgttaatat accttatgtg ttggttgagc
    11701 gccgagcagg cgacattgct gaatgttggt ccgacccgtc taaagcatac cttgaacttg
    11761 gatggaaagc ctgtttggga ttagatgaga tggttcgcga tgcctggaat tggcaacaaa
    11821 gcaatcctga tggttataaa ttttctgaga aaaactgaat tgcttagcct cagtaaaaat
    11881 ataatacttt tctaacctga gttaccgtaa gcttcatata gacctgcgtt aaagttcgca
    11941 gtaacccccg
//
