LOCUS       BGC0000791             12875 bp    DNA     linear   BCT 17-JUN-2008
DEFINITION  Enterobacter sakazakii strain NCTC 11467 O-antigen gene cluster,
            complete sequence.
ACCESSION   BGC0000791
VERSION     BGC0000791
KEYWORDS    .
SOURCE      Cronobacter sakazakii
  ORGANISM  Cronobacter sakazakii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Cronobacter.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 14:12:57
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 12875
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     gene            1..891
                     /gene="galF"
     CDS             1..891
                     /codon_start=1
                     /gene="galF"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) NTP_transf_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1064:glucose-1-phosphate
                     adenylyl/thymidylyltransferase (Score: 188.1; E-value:
                     3.3e-57)"
                     /gene_kind="biosynthetic-additional"
                     /product="UDP-glucose pyrophosphorylase"
                     /protein_id="ABX51871.1"
                     /sec_met_domain="NTP_transf_3 (E-value: 4.2e-10, bitscore:
                     30.0, seeds: 297, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEIV
                     AAGIKEIVLVTHSSKNAVENHFDTSYELEALLEQRVKRQLLAEVQSICPPGVTIMNVRQ
                     AQPLGLGHSILCARPIIGDNPFVVVLPDVIIDAASADPLRYNLAAMIARFQENGRSQVL
                     AKRMAGDLSEYSVITTQDPLDTEGKISRIVDFIEKPDQPQTLNSDLMAVGRYVLSADVW
                     EELAKTEPGAWGRIQLTDAIARLNENQPVEAMLMTGESYDCGKKMGYMKAFVNYGLRNL
                     KEGQKFRESIKKLLA"
     source          1..12875
                     /db_xref="taxon:28141"
                     /mol_type="genomic DNA"
                     /organism="Cronobacter sakazakii"
                     /strain="NCTC 11467"
                     /type_material="type strain of Cronobacter sakazakii"
     misc_feature    1..12875
                     /note="O-antigen gene cluster"
     subregion       1..12875
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000791"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..12875
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            1282..2361
                     /gene="rmlB"
     CDS             1282..2361
                     /NRPS_PKS="Domain: NAD_binding_4 (4-199). E-value: 1.7e-15.
                     Score: 48.6. Matches aSDomain:
                     nrpspksdomains_rmlB_NAD_binding_4.1"
                     /codon_start=1
                     /gene="rmlB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) NAD_binding_4"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010:NAD-dependent epimerase/dehydratase (Score:
                     288.9; E-value: 8.7e-88)"
                     /gene_kind="biosynthetic-additional"
                     /product="dTDP-D-glucose-4,6-dehydratase"
                     /protein_id="ABX51872.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 3.3e-20, bitscore:
                     62.0, seeds: 37, tool: rule-based-clusters)"
                     /sec_met_domain="Polysacc_synt_2 (E-value: 3.7e-18,
                     bitscore: 55.4, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="NAD_binding_4 (E-value: 4e-16, bitscore:
                     48.6, seeds: 102, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKILVTGGAGFIGSAVVRHIINHTQDRVVNLDKLTYAGNPESVAS
                     VANSERYAFEQVDICDRAALDRVFKEHQPDAVMHLAAESHVDRSISGPADFIETNIVGT
                     YTLLEAARAYWSSLDEERKAAFRFHHISTDEVYGDLPHPDEWHESAALPLFTETTAYAP
                     SSPYSASKASSDHLVRAWLRTYGLPTMVTNCSNNYGPYHFPEKLIPLVILNALEGKPLP
                     IYGKGDQIRDWLYVEDHARALYTVVTQGQIGETYNIGGHNEKKNLDVVLTICDLLDEIK
                     PKATSYREQITYVADRPGHDRRYAIDADKISRELGWRPQETFESGIRKTVHWYLNNEEW
                     VNSIKNRTYQCKHLGLSEK"
     CDS_motif       1291..1368
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_rmlB_0001"
                     /evalue="4.90E-05"
                     /label="PKSI-KR_m1"
                     /locus_tag="rmlB"
                     /protein_end="29"
                     /protein_start="3"
                     /score="16.0"
                     /tool="antismash"
                     /translation="LVTGGAGFIGSAVVRHIINHTQDRVV"
     aSDomain        1294..1878
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_rmlB_NAD_binding_4.1"
                     /evalue="1.70E-15"
                     /label="rmlB_NAD_binding_4.1"
                     /locus_tag="rmlB"
                     /protein_end="199"
                     /protein_start="4"
                     /score="48.6"
                     /tool="antismash"
                     /translation="VTGGAGFIGSAVVRHIINHTQDRVVNLDKLTYAGNPESVASVANS
                     ERYAFEQVDICDRAALDRVFKEHQPDAVMHLAAESHVDRSISGPADFIETNIVGTYTLL
                     EAARAYWSSLDEERKAAFRFHHISTDEVYGDLPHPDEWHESAALPLFTETTAYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTMVTNCSNNYG"
     gene            2358..3230
                     /gene="rmlA"
     CDS             2358..3230
                     /codon_start=1
                     /gene="rmlA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) NTP_transf_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1064:glucose-1-phosphate
                     adenylyl/thymidylyltransferase (Score: 273.7; E-value:
                     3e-83)"
                     /gene_kind="biosynthetic-additional"
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="ABX51873.1"
                     /sec_met_domain="NTP_transf_3 (E-value: 1.3e-13, bitscore:
                     41.4, seeds: 297, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKGIILAGGSGTRLYPITIGISKQLLPVYDKPMIYYPLSVLMLAG
                     IKDILIITTSDDNASFQRLLGDGSQFGINIQYAVQPSPDGLAQAFIIGRHFVGNDSVCL
                     VLGDNIFFGQGFSPKLKSAAAQKSGATIFAYQVKDAERFGVVEFDEQFKAISIEEKPKN
                     PKSNWAVTGLYFYDNDVLEIAKSIRPSERGELEITSVNEVYLNKQSLSVELLGRGFAWL
                     DTGTHDSLIEAGSFVETVQKRQGMMVACPEEIAWRNGWLSNIELEDIGQRLCKNDYGHY
                     LLQLLKKGE"
     gene            3232..3633
                     /gene="fdtA"
     CDS             3232..3633
                     /codon_start=1
                     /gene="fdtA"
                     /product="dTDP-6-deoxy-3,4-keto-hexulose isomerase"
                     /protein_id="ABX51874.1"
                     /transl_table=11
                     /translation="MDIKLIPLQTHGDERGSLVSLEDEKNIPFAIKRVYYLFNTKENVS
                     RGFHAHQRLKQVAIAIKGSCRFKLDDGKERVEIVLDSPSQGLYIDSFMWREMSDFSPDC
                     VLLILADSHYDESDYIRDYSLFKKMVTNG"
     gene            3626..4078
                     /gene="fdtC"
     CDS             3626..4078
                     /codon_start=1
                     /gene="fdtC"
                     /product="acetyltransferase"
                     /protein_id="ABX51875.1"
                     /transl_table=11
                     /translation="MDRITLTSYDREFLDLSWIWLQDPEIKKLTLTSDFTREQQLQFFH
                     SLKERKDYLIFGIQLGTRKIGVAGLKNIKNDIAEYWGYIGEKDLWGKGIGKIVIEEIEC
                     IANKCGIKKLYLKVSELNLRAIKAYHKSGFIISKSESNVIFMEKVI"
     gene            4079..5014
                     /gene="wehA"
     CDS             4079..5014
                     /codon_start=1
                     /gene="wehA"
                     /product="hypothetical protein"
                     /protein_id="ABX51876.1"
                     /transl_table=11
                     /translation="MFSICELEKSKYNDWNQVVENAKNGLFLFEIDYFKYHEARFEDSS
                     LMIYKKSKAVALFPANRVNDTIYSHQGLTFGGLIMLPELHAADVLEIFELINKHYKNKG
                     IKKVVYKAIPHPFTKYPAQEDLYALFINSAKLIRRDVSSVIDLFNRPKLSDSRKNTIVK
                     AQKNNVEICESSDLKKFHQILSEVLAKFGACPVHSITELEQLKNSFPHNIKLYAAMQDE
                     QWLAGVLVYDYGHIIHTQYMANTQQGRQIGALDYILFTLINDIYADKKYLSFGISTENA
                     GQYLNTGLIAQKEGFGARAVVHDFYEMELL"
     gene            5011..6114
                     /gene="fdtB"
     CDS             5011..6114
                     /NRPS_PKS="Domain: Aminotran_5 (34-168). E-value: 4e-09.
                     Score: 27.7. Matches aSDomain:
                     nrpspksdomains_fdtB_Aminotran_5.1"
                     /codon_start=1
                     /gene="fdtB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DegT_DnrJ_EryC1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1056:DegT/DnrJ/EryC1/StrS aminotransferase (Score:
                     313.7; E-value: 2.9e-95)"
                     /gene_kind="biosynthetic-additional"
                     /product="aminotransferase"
                     /protein_id="ABX51877.1"
                     /sec_met_domain="DegT_DnrJ_EryC1 (E-value: 7.1e-110,
                     bitscore: 357.3, seeds: 38, tool: rule-based-clusters)"
                     /sec_met_domain="Aminotran_1_2 (E-value: 6.3e-08, bitscore:
                     21.8, seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="Aminotran_5 (E-value: 1.9e-08, bitscore:
                     23.4, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINFLNLKKINEKYKTELMQALENVIDSGWYINGQQVANFENEFA
                     TYCGTKHAIGVANGLDALVLTIKAWKLLGKLKDGDKVIVPANTYIASVLAITENNLTPV
                     LTEPDPYTFNLTLDQIERSYTQDVKLIIPVHLYGCINPMDEICRFANKKGILVLEDSAQ
                     AHGAMLGDKKAGAWGHASAFSFYPGKNLGALGDGGIITTDNDELKEALVALRNYGSHKK
                     YENIYQGCNSRLDEIQAAMLRVKLPHLDSEIDARRRIASQYLLGINNKEIILPTVNESV
                     NHVWHLFVVRCAQRDALKEYLHKKNIDTLIHYPVPPHKQKAYSEYNSLNLPVTEKIHRE
                     VLSLPMDPNMSQYEVDYVIQCMNEYKA"
     aSDomain        5113..5514
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_fdtB_Aminotran_5.1"
                     /evalue="4.00E-09"
                     /label="fdtB_Aminotran_5.1"
                     /locus_tag="fdtB"
                     /protein_end="168"
                     /protein_start="34"
                     /score="27.7"
                     /tool="antismash"
                     /translation="QQVANFENEFATYCGTKHAIGVANGLDALVLTIKAWKLLGKLKDG
                     DKVIVPANTYIASVLAITENNLTPVLTEPDPYTFNLTLDQIERSYTQDVKLIIPVHLYG
                     CINPMDEICRFANKKGILVLEDSAQAHGAM"
     gene            6111..7373
                     /gene="wzx"
     CDS             6111..7373
                     /codon_start=1
                     /gene="wzx"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt"
                     /gene_kind="biosynthetic-additional"
                     /product="O-antigen flippase"
                     /protein_id="ABX51878.1"
                     /sec_met_domain="Polysacc_synt (E-value: 2.2e-09, bitscore:
                     26.9, seeds: 31, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSKIISVTAFTALLTFFKMLSGFAIAKVVAIYTGPTGMAMLGQVQ
                     SVIAGLNGVVTAPVGNGVVRYTAEKHTEGYKRCSGWWQASLQWAFSIFLLVLSTVLLFS
                     KPISQFLFNTIDYYWVVLLGCLVLPLSIINTAFVSVINGQKHYKKYVSLGFISVTISTV
                     LMVMMIYFYHIKGAFVAASLNTSIAGLVVFFACIKEPWFKVKLWLGRSSSSYRKEIGGY
                     VVMAVTSAITTPIALILIRNILISEVGWSATGQWQAVWKISEVYLAIITMALSTYYLPQ
                     LASLKSGVEIRWEINKTARVILPVVIILALLVYLLRDVAIFLLFTSDFQPARDLFSVQL
                     VGDVIKILAWLYAFPMLARGATRWFVFSEIFFSTTFVLISWIMISHLGVNGANWGYLLN
                     YILYFIFVYGNLKRIINQDRQ"
     gene            7373..8317
                     /gene="wehB"
     CDS             7373..8317
                     /codon_start=1
                     /gene="wehB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /product="glycosyl transferase"
                     /protein_id="ABX51879.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 4.1e-31,
                     bitscore: 97.9, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKDVVSVVIVTYNSAKTIVETLDSILNQSYGPANIELIISDDAST
                     DDTSIVVENWITKNASKFSKVVFKKNIINQGVSKNCNIGWQSCTSEWIKTIGADDLLSP
                     DCIDKNMEYIQNNSGCSIVFSQMKWFGRIKKITPEPYNMPFFSLDAEQQYNYLRFKSFN
                     IAPASFIRRSALIDVGYADESFSTIEDLPLWLRFTKKGYKLYFLKAITVYYRVANSASK
                     HSHRYVNYNFLNDLINIDKMFKINDIEGIYYKALKLDALTLLYGKKLIAKLTKNKVSFL
                     SRSLDVIHLSLRPVYIVRKIRRHIINFTKKDAI"
     gene            8356..9636
                     /gene="wzy"
     CDS             8356..9636
                     /codon_start=1
                     /gene="wzy"
                     /product="O-antigen polymerase"
                     /protein_id="ABX51880.1"
                     /transl_table=11
                     /translation="MFYLFVVSLFFGSCLDYYIWRKITPSMISVIGIYVILFIHAAIGE
                     SLGFLPVESNVYFFITTFYVVGLFASLSCAFGMSFLSKGIVLNTRVTDKYYGLKIRKNI
                     INITVVVSLICCFFIYKGWSEVGSFVSDDFEGTLTYGIAGHFFAILVALIPFLLKAYYS
                     KRTKSVFLLLVFVLVLLFMKQVKYWVMIPLVWMFWYSIVAGYIKLSIRKFLFISLVLAF
                     LLVSLFFSVYFMKVFFREGSENVDYNTVLIEIMIHFFGYLYSGLLTFSVYISDGLYNAF
                     IPSDILGLFSGPVNIINVILNDNLINLTLPKYFVVINKITGTTGNVPTLWGTLLLAGGY
                     YAYVFYFFMFFCIYLLLWFAKRSQCFLIIYTFITSFLFFSWFDYYYYLLMPFEVTVLIF
                     IFYTIFERKIKFRNITLFSGESYDETR"
     gene            9623..10501
                     /gene="wehC"
     CDS             9623..10501
                     /codon_start=1
                     /gene="wehC"
                     /product="glycosyl transferase"
                     /protein_id="ABX51881.1"
                     /transl_table=11
                     /translation="MRLDKKIILLCKSIKKSIYYFFRAIFSQSSGVDKNSTFLVTLTSY
                     GLRKNFVFLTIESILHQHVRPAKIVMWLHKKDKPQGIARWILSRQQKRGLEIFFVDNDV
                     RSYKKLSFVPGYYPDYDFYITADDDVFYPTNWIDGFKSNISDNPDAVYCYRGRAILFND
                     AGSLVAYPVWPLARRKEITGNELLPTGVSGVCYPRDSLDARVADYEAINKLCPYADDIW
                     YKALTTARGYNAILINDDSTHFTPIITGFTKGLEKINVLNDQNTQQFLNAMNYFNLDSN
                     SFRGNYKRNDE"
     gene            10491..11255
                     /gene="wehD"
     CDS             10491..11255
                     /codon_start=1
                     /gene="wehD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /product="glycosyl transferase"
                     /protein_id="ABX51882.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 1e-40, bitscore:
                     129.2, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMSNLVSIIMPSWNSELTIADSINSIIRQTYTNWELIITDDCSSD
                     GTIRLLKEFANKEPRIKLFFNDKNSGAGISRNNCIKNAKGRFIAFLDSDDMWHPEKLSR
                     QVAFMLNNNYALTYTAYKKINQKGNVIGHINPPARVNYSELLKSNVIGCLTAIYDAQKL
                     GKVYMPEIRKRQDMALWLKILEKIDYAWCLPEELAFYREGHESLSSNKIKILSSQWSFY
                     RTYLKFNSIKSAWYFSFYVVRAVKKHGVNKSS"
     gene            11469..12875
                     /gene="gnd"
     CDS             11469..12875
                     /codon_start=1
                     /gene="gnd"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1100:3-hydroxyisobutyrate dehydrogenase (Score: 321.3;
                     E-value: 1e-97)"
                     /gene_kind="biosynthetic-additional"
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="ABX51883.1"
                     /transl_table=11
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSVFNRSRDKTEEVIAE
                     NPGKKLVPYYTVQEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLEKGDIIIDGGNTF
                     FQDTIRRNRELSEEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILKEIAAVA
                     EGEPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAEAYSLLKHGLNLSNEDLAKTFAEWN
                     EGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSSLDLGEPLSLITE
                     SVFARYISSLKEQRVAASKVLSGPQAKPFAGDKGEFIEKVRRALYLGKIVSYAQGFSQL
                     RAASEEYNWDLNYGQIAKIFRAGCIIRAQFLQKITDAYAENPSIANLLLAPYFKKAADD
                     YQQALRDVVAYAVQNGIPTPTFSAAIAYYDSYRSAVLPANLIQAQRDYFGAHTYKRTDK
                     EGVFHTEWME"
ORIGIN
        1 atggttaatt tgaaagcagt tattcccgtc gcagggctcg gcatgcatat gctgcctgcc
       61 accaaggcga ttccaaagga gatgctgccg attgtcgaca aaccgatgat tcaatacatt
      121 gtcgacgaaa tcgtagcggc agggatcaag gaaattgtgc tggttaccca ctcctccaag
      181 aacgcggttg agaaccactt cgacacctct tacgaactcg aagcgctgct tgaacagcgt
      241 gtgaagcgcc agcttcttgc ggaagtgcag tctatctgcc cgccgggcgt gaccatcatg
      301 aatgtgcgtc aggcgcagcc gctgggcctt ggtcactcta ttctgtgtgc gcgtccgatt
      361 atcggcgata acccgtttgt tgtcgtgctg ccggatgtta ttattgatgc cgcttctgct
      421 gacccactgc gttataacct ggccgctatg attgcgcggt tccaggaaaa tggccgcagc
      481 caggtgctgg cgaagcgtat ggcaggcgat ctctctgaat attcagtgat aactactcag
      541 gatccgctgg atactgaagg caaaatcagc cgtatcgttg atttcatcga gaaaccggac
      601 cagccgcaga cgctgaattc tgatctgatg gcggtcgggc gctatgtgct ctctgccgat
      661 gtctgggaag agcttgcgaa aaccgaaccg ggcgcctggg gacgtatcca gctgaccgat
      721 gctatcgctc gtctgaacga gaatcagcct gttgaagcga tgctgatgac cggcgaaagc
      781 tacgattgcg gtaagaaaat gggttacatg aaagcgttcg tcaattacgg cctgcgtaac
      841 ctgaaagaag ggcagaagtt ccgcgagagc attaagaaac tgctggcata agccagccgt
      901 tctctgcaga acgctataca ggcaggatag cctttgataa aacggcagtt gagcattcgg
      961 gttgataagc atttatactc gacggactcg ctgccgtttt gttttttggt gcaagcgctg
     1021 tgacggggcc ataatccgaa atagttaccg agatcgacta tcccgtcaaa aaagctaatt
     1081 agctgatttc aaaagttttg tataaagtag tgcactggta gctgttgagc caggggcggt
     1141 agcattgttg aacaggctgc cgaaaaaatg cttaatattt atccatatag agcgttaaaa
     1201 cctcgataac gtcatgctca cagcgacaat gtgttaatta tcattcctct caaaatatcc
     1261 acatataaga gataactcac tgtgaagata ttagtgaccg gcggtgccgg atttattggc
     1321 tccgctgtag tacgccatat tattaaccat acccaggaca gggtagttaa tcttgataag
     1381 cttacctacg caggcaatcc tgaatctgtt gcttctgtgg caaacagtga acgctatgca
     1441 tttgagcagg tcgatatctg tgaccgcgct gcgctcgatc gtgtatttaa agagcaccag
     1501 cctgacgcgg tcatgcacct ggctgcagaa agtcatgttg atcgctcaat cagcggtccg
     1561 gcagatttca tcgaaacgaa catcgtcggc acgtataccc ttctcgaagc ggcgcgcgct
     1621 tactggtcat cactggatga ggagcgcaag gcagctttcc gctttcacca tatctctacc
     1681 gatgaggttt atggcgacct gccacatcct gatgaatggc atgagagcgc ggcgctgccg
     1741 ctctttactg aaacaactgc ctatgcgcca agcagcccgt attccgcctc aaaagcctca
     1801 agcgatcatc tggtgcgcgc gtggttacgc acctatggtt tgccgacgat ggtaaccaac
     1861 tgctctaaca actatggccc gtatcacttc cccgaaaaat tgattccgct ggttatcctt
     1921 aacgcgctgg aaggtaaacc gttgccgata tatggcaaag gcgaccaaat tcgcgactgg
     1981 ttatatgttg aagaccatgc gcgtgcgctc tataccgtag tcacgcaggg gcagatcggc
     2041 gaaacctata atatcggcgg acataacgag aaaaagaatc ttgatgtcgt actgactatt
     2101 tgtgacctgc tggatgagat taaacctaaa gcgacgtctt atcgtgaaca gatcacctat
     2161 gttgccgaca gaccagggca cgaccgtcgt tatgccattg atgcagataa aatcagccgg
     2221 gaattaggct ggcgaccgca ggaaaccttc gagtctggca tccgtaaaac ggtgcactgg
     2281 tatcttaata atgaagagtg ggtgaacagc attaagaacc gaacctatca atgtaagcat
     2341 ttagggttga gtgaaaaatg aaaggaatta tcctggcagg tgggtccgga acacgtcttt
     2401 atccaattac tataggtatc tctaaacagt tacttcctgt atacgataaa cctatgattt
     2461 attatcctct atcagtcctc atgctggccg gtattaagga tattttaata atcactacta
     2521 gtgatgataa cgcgagtttc cagcgtttac ttggagatgg cagccagttt gggatcaata
     2581 ttcaatatgc tgttcaacca tctcccgatg ggcttgcaca ggcttttata ataggtcgtc
     2641 atttcgttgg taacgactcg gtttgtttgg ttcttggcga taatattttc tttgggcaag
     2701 gttttagccc taaactcaag tcagcggctg ctcaaaaaag tggtgcgacg atttttgctt
     2761 accaggtaaa agacgcagag cgctttggtg ttgttgaatt tgatgagcaa tttaaagcga
     2821 tctctatcga agaaaaacca aagaatccta agtctaactg ggcggtcact ggcctttatt
     2881 tttatgataa tgacgttttg gagattgcta aatctattcg cccttccgag cgaggtgaat
     2941 tagaaattac ctcagtcaat gaagtttatt taaataagca gagccttagt gtagagctct
     3001 tagggcgtgg tttcgcctgg cttgatactg gaacacacga tagtctcatc gaagctggaa
     3061 gttttgttga gacggtacag aagcgtcagg gaatgatggt tgcttgtcct gaggaaatag
     3121 cctggcgaaa tggatggctg agtaatattg agttagaaga tattgggcaa cgtttatgta
     3181 aaaatgacta tggccattac ctactacaac tattaaaaaa aggagaataa tatggatatt
     3241 aaactaattc cacttcagac gcatggtgat gaacgaggca gtttggtatc gcttgaagat
     3301 gaaaagaaca tcccttttgc aataaaacgt gtttattatt tatttaatac taaagaaaac
     3361 gtaagccgcg gattccatgc gcaccaaagg ttaaagcaag tggctattgc aataaaagga
     3421 tcgtgtcgat ttaaacttga tgatggtaaa gaacgagtag aaatcgttct ggattcgcct
     3481 tctcaaggtc tttacattga ttcttttatg tggcgagaaa tgtcagattt ttctcctgat
     3541 tgcgttctac ttatacttgc tgatagtcat tatgatgaat ctgactatat tcgggattat
     3601 tcactattta aaaaaatggt aaccaatgga tagaataact ttaacaagtt atgaccgtga
     3661 gtttttagat cttagctgga tatggctgca ggatccggaa ataaaaaaat taactttaac
     3721 cagtgatttt actcgtgagc agcaattaca attttttcat tccctaaagg aaagaaaaga
     3781 ttatttgata tttggtattc aattagggac gagaaaaatt ggtgttgccg ggttaaaaaa
     3841 catcaaaaac gatatcgcag aatactgggg ctatataggc gagaaggatt tatggggtaa
     3901 aggaattggc aaaattgtta tagaagaaat cgaatgtatt gccaataaat gtgggataaa
     3961 aaagctatac ttgaaagtta gtgaattaaa tctcagagct atcaaagctt accataaatc
     4021 tggttttata attagtaaat ctgaaagtaa tgtgattttt atggaaaagg taatttagat
     4081 gttttcgata tgtgaacttg aaaaaagtaa atataatgat tggaatcaag tggtcgaaaa
     4141 tgctaaaaac ggccttttcc tttttgagat agattatttt aaatatcatg aagcgcgatt
     4201 tgaagatagt tctttaatga tatataaaaa aagtaaagct gtcgcacttt tccctgctaa
     4261 ccgtgtgaat gacaccattt atagccacca aggattaact tttggcggtt tgattatgct
     4321 gccagaatta catgcagcgg atgttttaga aatatttgaa cttataaata aacattataa
     4381 aaacaaaggt attaaaaagg ttgtttataa agctatacct catcctttta caaagtatcc
     4441 tgcccaggaa gatttatacg cattattcat aaattctgca aaattaatcc gcagagacgt
     4501 ttcaagcgta attgatctct ttaacagacc taagctttca gattcgcgta agaataccat
     4561 tgttaaagca caaaaaaata atgtggagat ctgtgaatcg tctgacttga aaaaattcca
     4621 tcaaatactt tcagaggttc tggcaaaatt tggtgcatgc cctgtacata gcatcacaga
     4681 acttgaacaa ttaaaaaact catttccgca taatattaaa ctctatgccg ccatgcagga
     4741 tgagcagtgg ctcgctgggg ttctggtcta cgattacgga catattattc atacacaata
     4801 tatggccaat acacagcaag gtcggcaaat aggcgcacta gattatatat tatttacgtt
     4861 aatcaatgac atttatgctg ataaaaaata tctaagtttt ggtatatcaa ctgaaaacgc
     4921 aggacaatat ctcaatactg gattaatcgc tcaaaaagaa ggtttcggcg ccagagcggt
     4981 tgtacatgat ttttatgaaa tggagttatt atgattaact ttttaaattt aaaaaaaata
     5041 aacgaaaaat ataaaaccga attgatgcag gcattggaaa acgtgatcga ttctggttgg
     5101 tatatcaatg gccaacaggt agctaatttt gaaaatgaat ttgctaccta ttgtggtaca
     5161 aaacatgcca tcggggttgc aaatggactg gatgcattag tccttactat aaaggcatgg
     5221 aagctattag gaaaacttaa ggacggtgac aaagttattg tccctgcaaa tacctatata
     5281 gcttcagtat tagcgatcac cgaaaataat ttaacgcctg ttcttacaga gccagatcct
     5341 tacactttta atttaacgct agatcaaatc gaaagaagtt atacgcagga tgtaaaatta
     5401 atcattcctg tacatctcta tggatgcatc aacccaatgg atgagatctg tagattcgca
     5461 aataaaaaag gaatattggt tttagaagat tcagcgcaag cgcatggggc aatgcttgga
     5521 gataaaaaag ctggtgcctg gggtcatgca tcagccttta gtttttatcc cggtaaaaat
     5581 ttaggtgctt taggcgatgg tgggatcatc acgactgaca acgatgaact aaaagaagca
     5641 ttagtggcac tcaggaatta tggatctcac aaaaaatatg aaaatattta tcagggatgc
     5701 aatagccgac tagatgaaat tcaggctgca atgcttcgag ttaaattacc tcatttagat
     5761 agcgaaatag atgctcgcag aagaattgcc tcccaatatc ttttaggcat aaataataaa
     5821 gaaataatac ttcccactgt aaatgaaagt gtgaatcatg tctggcatct atttgtcgtt
     5881 cgctgtgcgc aaagagatgc attgaaagaa tacttacata agaaaaatat tgatacgcta
     5941 atacattatc ctgttccccc tcataagcag aaggcttaca gtgaatataa tagtcttaat
     6001 cttcctgtta cagaaaaaat ccatcgtgaa gtactttctt tgccaatgga tccaaatatg
     6061 tctcagtacg aggtggatta tgttattcag tgcatgaatg agtataaggc atgagtaaaa
     6121 tcatctctgt tactgctttt acagccttac tgacattctt caaaatgtta agtggttttg
     6181 cgatagctaa ggtcgttgct atatatacgg ggcctactgg aatggctatg cttgggcagg
     6241 tacaaagtgt gattgctggg cttaatggtg ttgtgactgc acccgtaggg aatggggtgg
     6301 ttcgctatac ggctgaaaag catactgaag gttataaaag atgttcagga tggtggcagg
     6361 cgtccttaca gtgggcattt tctatatttt tgctagtgct gagcaccgta ttattattta
     6421 gcaaacctat ttctcaattt ctattcaata ctattgatta ttattgggtt gtactattag
     6481 gttgtttagt tcttccattg tcaataataa atacggcgtt tgtttctgtt attaacggac
     6541 agaaacacta taaaaaatat gtctcgctag ggtttatttc cgtaaccata tcgactgtat
     6601 taatggttat gatgatttat ttttatcata ttaaaggtgc ttttgttgct gcatcactta
     6661 atacatcgat tgccggacta gtcgtttttt ttgcttgtat aaaggagcct tggtttaaag
     6721 ttaaattatg gcttggccgc tcatcttcgt cttatagaaa agaaattgga ggctatgtag
     6781 tgatggcagt tacatccgcc atcactactc cgattgcatt gattctgata agaaatatat
     6841 taatttccga agtgggttgg agtgcgacag gacaatggca agcagtatgg aaaatctctg
     6901 aagtttatct tgccattatt actatggcat tgagtacata ttatttgcct caacttgcaa
     6961 gcttgaagag tggtgttgag atacggtggg aaataaataa aacagcaaga gttattcttc
     7021 ctgtggttat tattttagcg ttgttagttt atttgcttcg cgatgtcgct atatttttac
     7081 tttttacaag cgattttcaa cctgcgagag acttattcag cgttcaactt gtgggggacg
     7141 tcatcaaaat cctggcgtgg ctatatgctt ttccgatgtt agccagaggc gctactcgct
     7201 ggttcgtgtt ttctgaaata tttttttcca caacattcgt gttaataagt tggattatga
     7261 tttcgcatct gggagttaat ggtgcaaatt ggggatactt attgaattac attttatact
     7321 ttatctttgt atatggtaat ttaaaaagaa taattaacca ggataggcaa taatgaaaga
     7381 tgttgtttca gtagtaattg ttacttataa ctcagcgaaa actatagtag aaactttaga
     7441 tagtatttta aatcaaagtt atgggcctgc taatatagag cttatcatat cagatgatgc
     7501 ttctactgat gacacgtcaa tcgttgttga aaactggatt actaaaaacg catcaaaatt
     7561 ttcaaaggtt gttttcaaaa aaaatattat caatcagggc gtttctaaaa attgtaacat
     7621 tggctggcaa tcctgcacat ctgaatggat aaaaacaata ggcgcagatg atcttctaag
     7681 tcctgattgc attgataaaa acatggaata tattcagaat aattccgggt gtagtattgt
     7741 atttagtcag atgaaatggt tcggacggat aaaaaaaata actcctgagc catataatat
     7801 gccttttttt agcctggatg ctgaacaaca atataattat ttaagattta aaagcttcaa
     7861 tattgcacct gcatcattta tacgtcgttc agctctaatt gatgttggtt atgctgacga
     7921 aagtttcagt acgattgaag acttaccatt atggcttcgc ttcactaaaa agggctacaa
     7981 actgtatttt ttgaaagcga ttactgtgta ctaccgtgtg gcaaattctg catcgaaaca
     8041 ttctcaccgt tatgttaact ataacttttt gaacgatctg attaatatcg acaagatgtt
     8101 taaaattaac gatatcgaag gtatttatta taaagcgcta aaactggatg ctctaacatt
     8161 attatatgga aaaaagttaa ttgcaaagtt aaccaagaat aaagtcagct ttttgtcgag
     8221 atcgctggat gtcatacatt taagcttaag acctgtttat attgttcgga agattaggcg
     8281 acatatcatt aactttacta aaaaagatgc aatctaaatt atattgcatc tacttttata
     8341 aaaattggtg taaatatgtt ttatttattt gttgtgtctt tgtttttcgg ttcgtgtctt
     8401 gattattata tatggcgaaa aattacgcct tcaatgattt ctgttatcgg tatatatgta
     8461 atattgttta tacatgctgc cataggcgag tctcttggtt ttttacctgt agagtcaaat
     8521 gtttactttt ttataacaac attttatgtt gtcgggcttt ttgcaagtct aagctgtgca
     8581 tttgggatgt catttctcag caaaggcatt gttcttaata ctcgtgtgac agataaatat
     8641 tacggactta aaattcggaa aaatataata aatataacag tagtggtttc tcttatatgt
     8701 tgttttttta tttataaagg atggagtgag gttggatctt ttgtaagcga tgattttgag
     8761 gggactctca cttatggcat cgcgggtcat ttttttgcta tcctggttgc tttaatacca
     8821 tttttgctaa aagcatatta ctctaaaaga accaaatccg ttttcttact gttagttttt
     8881 gtgcttgtgc ttttatttat gaagcaagtt aaatattggg taatgatccc gcttgtatgg
     8941 atgttttggt actctatagt tgctggatat ataaagctct ctattagaaa gttcttgttt
     9001 atttcgctgg ttttggcatt tttactcgtt tctttatttt tctcagtcta ttttatgaag
     9061 gtttttttta gagaaggttc tgaaaacgtt gattataata ctgtattaat agaaataatg
     9121 attcatttct ttggctatct ttattcggga cttctaacat tttctgtata catttctgat
     9181 ggtttatata atgcatttat accaagcgac attcttgggc ttttttccgg tccggtcaat
     9241 ataatcaatg taatactaaa tgacaacttg ataaacctaa cgctcccaaa gtattttgtc
     9301 gtaatcaata aaattactgg tacgactgga aatgttccaa cgctatgggg tactctgttg
     9361 ctggccggag ggtattatgc atatgttttc tatttcttca tgtttttttg catatattta
     9421 ttgctgtggt ttgcgaagcg tagtcaatgt tttttaatta tatatacgtt tattacgtcg
     9481 tttctttttt ttagttggtt tgattactac tactacctgt taatgccgtt tgaagttact
     9541 gttttaattt ttattttcta tactatcttt gaaaggaaaa taaagttcag aaatataact
     9601 ttattctctg gggaaagtta cgatgagact cgataaaaaa attatactgt tatgcaagtc
     9661 aataaaaaaa agcatatatt actttttcag agcgattttt tctcaatcat ctggggttga
     9721 caaaaattcg acatttttag taacgctaac tagctatggt ttacgtaaga attttgtttt
     9781 cttgacaatt gagagtatct tgcatcaaca cgttcgccct gcaaaaatag taatgtggtt
     9841 acataaaaag gataaacctc agggaatagc gagatggatt ttatcccgac agcaaaagcg
     9901 agggctggaa atattttttg tagataatga tgttagatct tataaaaaac tttcttttgt
     9961 acctggttat tatcctgatt acgatttcta tattaccgct gacgacgacg tattttaccc
    10021 aacaaattgg atcgatggtt ttaaatctaa tataagtgat aatcctgatg ctgtttactg
    10081 ttacagaggc cgtgctattc tgtttaacga tgcaggtagt ctcgtcgctt atccagtctg
    10141 gccgcttgcg cgaagaaaag aaataacagg caatgagtta cttcccacag gtgtatcagg
    10201 cgtgtgttat cctagagatt cgctcgacgc acgtgtagcc gattacgagg cgataaataa
    10261 actgtgtccc tatgccgatg acatttggta taaagcgcta actacagctc gcggatataa
    10321 cgctattttg atcaatgatg acagtacgca ttttacaccg attatcactg gcttcacaaa
    10381 aggcctcgag aagataaacg tactaaatga tcagaatacg caacaatttt taaacgctat
    10441 gaattatttt aatcttgatt cgaattcttt tagaggcaat tataaaagaa atgatgagta
    10501 atcttgtatc aattattatg cctagctgga attctgagtt aactatcgct gatagtataa
    10561 attcaatcat taggcaaacc tataccaact gggaattaat cataactgat gattgttcga
    10621 gtgatggcac tatccgtttg ttaaaagagt ttgctaataa agaaccacga ataaaactat
    10681 ttttcaacga taagaattca ggcgctggta tatcccgtaa taattgtatt aaaaatgcaa
    10741 aagggcgctt tattgctttt ttggatagtg atgatatgtg gcatccggaa aaactgtcca
    10801 gacaagtagc cttcatgctt aacaacaact atgcattgac ttacactgca tataaaaaaa
    10861 tcaatcagaa aggtaacgta ataggccata tcaatcctcc agcacgagtt aactattctg
    10921 agcttttaaa atccaatgtt ataggatgcc taactgcgat atatgatgct caaaagcttg
    10981 gtaaggttta tatgcctgaa attcgcaaac gccaggatat ggctttatgg ctaaaaatac
    11041 tagaaaaaat tgattatgcc tggtgtttac cagaagaact tgcgttttat agagagggac
    11101 atgaaagtct ttcttcaaat aagataaaaa ttctttcatc acaatggtcg ttttacagaa
    11161 cttaccttaa atttaattct ataaagtcag catggtattt ttcattctat gttgttcgag
    11221 cagtaaaaaa acatggcgtc aataaaagta gctaaagcac tttttaatta taaatgtttt
    11281 gactttatta actttgaaat cctattcgga atgccctgga acgataaggt ttaccaatga
    11341 gcattcttcg ctcagttata actttgctgt tatcaatttc tattcaaatt cgctatctat
    11401 gagttaacat cgtagttaca taacacccgc gcacaagtcg cggtgaccac acctgacagg
    11461 agtatgtaat gtccaagcaa cagatcggcg tcgtcggtat ggcagtcatg gggcgcaacc
    11521 tggcgctcaa cattgaaagc cgtggttata ccgtctccgt attcaaccgc tctcgtgaca
    11581 aaactgaaga agttattgcc gaaaatccgg gcaaaaaact ggttccttat tataccgttc
    11641 aggagtttgt cgagtcgctg gaaaccccgc gccgtatcct gctgatggtg aaagcaggtg
    11701 ccggtactga tgcggctatc gactccctga agccttatct ggaaaaaggc gacatcatta
    11761 ttgatggcgg taacaccttc ttccaggaca ccatccgtcg taaccgtgaa ctctccgaag
    11821 aaggctttaa ctttatcggc accggcgtct ccggtggcga agagggcgcc ctgaaaggcc
    11881 cgtccatcat gcctggcggc cagaaagaag cttatgagct ggttgcgccg atcctcaaag
    11941 agatcgcggc cgtagcggaa ggtgaaccgt gcgtgactta tatcgggcca gacggcgcgg
    12001 gtcactacgt gaaaatggtg cacaacggta tcgaatacgg cgatatgcag cttattgcag
    12061 aagcctattc cctgctgaag cacggtctga acctgtctaa cgaagatctg gcgaaaacct
    12121 tcgctgagtg gaacgaaggc gagctcagca gctaccttat tgatatcacc aaagatatct
    12181 tcaccaaaaa agatgaagac ggtaactacc tggttgacgt cattctggac gaagccgcca
    12241 acaaaggtac tggtaagtgg accagccaga gctcgctgga tctcggcgaa ccgctgtccc
    12301 tgattactga atcggtattc gcgcgttata tctcctctct gaaagagcag cgcgtcgcgg
    12361 catccaaagt gctgagcggc ccgcaggcga aaccgttcgc aggcgataaa ggcgaattta
    12421 tcgagaaagt ccgccgcgcg ctgtacctcg gtaaaatcgt ctcttacgcg cagggcttct
    12481 ctcagctgcg cgctgcgtct gaagagtaca actgggatct gaactacggc caaatcgcga
    12541 agatcttccg cgcgggctgc atcattcgtg ctcagttcct gcagaaaatt actgacgcct
    12601 acgcagagaa tccgtccatc gcgaacctgc tgctggcgcc gtacttcaag aaagccgctg
    12661 atgactatca gcaggcgctg cgcgatgttg tggcgtatgc cgtacagaac ggtatcccga
    12721 ctccgacctt ctccgccgct atcgcttact acgacagcta tcgttctgcg gtgctgccag
    12781 cgaacctgat tcaggcacag cgtgactact ttggcgcgca tacctacaag cgcaccgata
    12841 aagaaggcgt attccacacc gagtggatgg aataa
//
