Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0000805.1:
Cluster 1Saccharide14726

BGC0000805, moenomycin biosynthetic gene cluster from Streptomyces ghanaensis. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: moenomycin
PubChem ID: 46926246
SMILES string: Copy to clipboard
Molecular formula: C69H104N4O35P-3
Average molecular mass: 1580.558 Da
Molecular activity: Antibacterial
Molecular target: peptidoglycan glycosyltransferases

Class-specific details

Biosynthetic class(es):
Saccharide
Saccharide subclass:
other
Glycosyltransferases:
ABJ90158.1:
Specificity: D-galacturonic acid
Evidence for specificity: Activity assay
Sugar biosynthesis subcluster: MOEE5
ABJ90161.1:
Specificity: N-acetyl-D-glucosamine
Evidence for specificity: Knock-out construct
ABJ90167.1:
Specificity: chinovosamine AND N-acetyl-D-glucosamine
Evidence for specificity: Knock-out construct
Sugar biosynthesis subcluster: MOER5, MOES5
ABJ90168.1:
Specificity: D-galacturonic acid
Evidence for specificity: Knock-out construct
ABJ90169.1:
Specificity: D-glucose
Evidence for specificity: Knock-out construct

Gene cluster description

moenomycin (BGC0000805). Gene Cluster 1. Biosynthetic class = Saccharide. GenBank DQ988993, positions 898-5623. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Homologous known gene clusters

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Knock-out studies, Enzymatic assays, Heterologous expression, Proven expression in natural host, Sequence-based prediction
MIxS-compliance:Unknown
Contact for this cluster:Bohdan Ostash (Ivan Franko National University of Lviv)

Literature references

1. Ostash B et al. (2007) A streamlined metabolic pathway for the biosynthesis of moenomycin A. Chem Biol 14(3):257-67. doi: 10.1016/j.chembiol.2007.01.008.
2. Ostash B et al. (2009) Complete characterization of the seventeen step moenomycin biosynthetic pathway. Biochemistry 48(37):8830-41. doi: 10.1021/bi901018q.
3. Ostash B et al. (2013) MoeH5: a natural glycorandomizer from the moenomycin biosynthetic pathway. Mol Microbiol 90(6):1324-38. doi: 10.1111/mmi.12437. Epub 2013 Nov 21.
4. Gampe CM et al. (2011) Modular synthesis of diphospholipid oligosaccharide fragments of the bacterial cell wall and their use to study the mechanism of moenomycin and other antibiotics. Tetrahedron 67(51):9771-9778. doi: 10.1016/j.tet.2011.09.114.