Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0000808.1:
Cluster 1Saccharide114771
The following clusters are from record BGC0000808.2:
Cluster 2Saccharide114064

BGC0000808, cepacian biosynthetic gene cluster from Burkholderia multivorans. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: Cepacian
Molecular activity: Other

Class-specific details

Biosynthetic class(es):
Saccharide
Saccharide subclass:
exopolysaccharide
Glycosyltransferases:
BMUL_4914:
Specificity: Unknown
Evidence for specificity: Unknown
BMUL_4913:
Specificity: Unknown
Evidence for specificity: Unknown
BMUL_4911:
Specificity: Unknown
Evidence for specificity: Unknown
BMUL_4910:
Specificity: Unknown
Evidence for specificity: Unknown
BMUL_4608:
Specificity: Unknown
Evidence for specificity: Unknown
Unknown gene ID
Specificity: None
Evidence for specificity: Unknown

Gene cluster description

cepacian (BGC0000808). Gene Cluster 1. Biosynthetic class = Saccharide. GenBank CP000869, positions 2031936-2046706. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Homologous known gene clusters

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Knock-out studies, Enzymatic assays, Sequence-based prediction
MIxS-compliance:Unknown
Contact for this cluster:Leonilde Moreira (Institute for Bioengineering and Biosciences, Instituto Superior Tecnico, Universidade de Lisboa)

Literature references

1. Moreira LM et al. (2003) Identification and physical organization of the gene cluster involved in the biosynthesis of Burkholderia cepacia complex exopolysaccharide. Biochem Biophys Res Commun 312(2):323-33.
2. Ferreira AS et al. (2010) Distribution of cepacian biosynthesis genes among environmental and clinical Burkholderia strains and role of cepacian exopolysaccharide in resistance to stress conditions. Appl Environ Microbiol 76(2):441-50. doi: 10.1128/AEM.01828-09. Epub
3. Sousa SA et al. (2007) The Burkholderia cepacia bceA gene encodes a protein with phosphomannose isomerase and GDP-D-mannose pyrophosphorylase activities. Biochem Biophys Res Commun 353(1):200-6. doi:
4. Videira PA et al. (2005) Functional and topological analysis of the Burkholderia cenocepacia priming glucosyltransferase BceB, involved in the biosynthesis of the cepacian exopolysaccharide. J Bacteriol 187(14):5013-8. doi: 10.1128/JB.187.14.5013-5018.2005.
5. Ferreira AS et al. (2007) Functional analysis of Burkholderia cepacia genes bceD and bceF, encoding a phosphotyrosine phosphatase and a tyrosine autokinase, respectively: role in exopolysaccharide biosynthesis and biofilm formation. Appl Environ Microbiol 73(2):524-34. doi: 10.1128/AEM.01450-06. Epub
6. Sousa SA et al. (2013 TI) Biochemical and functional studies on the Burkholderia cepacia complex bceN gene, encoding a GDP-D-mannose 4,6-dehydratase. PLoS One 8(2):e56902. doi: 10.1371/journal.pone.0056902. Epub 2013 Feb 27.
7. Ferreira AS et al. (2011 TI) Insights into the role of extracellular polysaccharides in Burkholderia adaptation to different environments. Front Cell Infect Microbiol 1:16. doi: 10.3389/fcimb.2011.00016.

BGC0000808, cepacian biosynthetic gene cluster from Burkholderia multivorans. Locus 2. Full MIBiG entry.

Chemical compounds

Compound: Cepacian
Molecular activity: Other

Class-specific details

Biosynthetic class(es):
Saccharide
Saccharide subclass:
exopolysaccharide
Glycosyltransferases:
BMUL_4914:
Specificity: Unknown
Evidence for specificity: Unknown
BMUL_4913:
Specificity: Unknown
Evidence for specificity: Unknown
BMUL_4911:
Specificity: Unknown
Evidence for specificity: Unknown
BMUL_4910:
Specificity: Unknown
Evidence for specificity: Unknown
BMUL_4608:
Specificity: Unknown
Evidence for specificity: Unknown
Unknown gene ID
Specificity: None
Evidence for specificity: Unknown

Gene cluster description

cepacian (BGC0000808). Gene Cluster 2. Biosynthetic class = Saccharide. GenBank CP000869, positions 2031936-2046706. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Homologous known gene clusters

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Knock-out studies, Enzymatic assays, Sequence-based prediction
MIxS-compliance:Unknown
Contact for this cluster:Leonilde Moreira (Institute for Bioengineering and Biosciences, Instituto Superior Tecnico, Universidade de Lisboa)

Literature references

1. Moreira LM et al. (2003) Identification and physical organization of the gene cluster involved in the biosynthesis of Burkholderia cepacia complex exopolysaccharide. Biochem Biophys Res Commun 312(2):323-33.
2. Ferreira AS et al. (2010) Distribution of cepacian biosynthesis genes among environmental and clinical Burkholderia strains and role of cepacian exopolysaccharide in resistance to stress conditions. Appl Environ Microbiol 76(2):441-50. doi: 10.1128/AEM.01828-09. Epub
3. Sousa SA et al. (2007) The Burkholderia cepacia bceA gene encodes a protein with phosphomannose isomerase and GDP-D-mannose pyrophosphorylase activities. Biochem Biophys Res Commun 353(1):200-6. doi:
4. Videira PA et al. (2005) Functional and topological analysis of the Burkholderia cenocepacia priming glucosyltransferase BceB, involved in the biosynthesis of the cepacian exopolysaccharide. J Bacteriol 187(14):5013-8. doi: 10.1128/JB.187.14.5013-5018.2005.
5. Ferreira AS et al. (2007) Functional analysis of Burkholderia cepacia genes bceD and bceF, encoding a phosphotyrosine phosphatase and a tyrosine autokinase, respectively: role in exopolysaccharide biosynthesis and biofilm formation. Appl Environ Microbiol 73(2):524-34. doi: 10.1128/AEM.01450-06. Epub
6. Sousa SA et al. (2013 TI) Biochemical and functional studies on the Burkholderia cepacia complex bceN gene, encoding a GDP-D-mannose 4,6-dehydratase. PLoS One 8(2):e56902. doi: 10.1371/journal.pone.0056902. Epub 2013 Feb 27.
7. Ferreira AS et al. (2011 TI) Insights into the role of extracellular polysaccharides in Burkholderia adaptation to different environments. Front Cell Infect Microbiol 1:16. doi: 10.3389/fcimb.2011.00016.