LOCUS       BGC0000815             25344 bp    DNA     linear   PLN 28-OCT-2008
DEFINITION  Neotyphodium uncinatum isolate e167 LOL-1 loline alkaloid production
            gene cluster, complete sequence.
ACCESSION   BGC0000815
VERSION     BGC0000815
KEYWORDS    .
SOURCE      Epichloe uncinata
  ORGANISM  Epichloe uncinata
            Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina;
            Sordariomycetes; Hypocreomycetidae; Hypocreales; Clavicipitaceae;
            Epichloe.
COMMENT     On or before Dec 14, 2004 this sequence version replaced
            AF461176.1, AF439394.1.
            ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2022-11-07 20:42:23
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 25344
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..25344
                     /db_xref="taxon:5050"
                     /isolate="e167"
                     /mol_type="genomic DNA"
                     /organism="Epichloe uncinata"
                     /strain="CBS 102646"
     subregion       1..25344
                     /aStool="mibig"
                     /contig_edge="True"
                     /label="Alkaloid"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..25344
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     mRNA            complement(join(3316..3448,3509..4960,5028..5135))
                     /allele="1"
                     /gene="lolF"
                     /product="LolF-1"
     gene            complement(3316..5135)
                     /allele="1"
                     /gene="lolF"
     CDS             complement(join(3346..3448,3509..4960,5028..5095))
                     /allele="1"
                     /codon_start=1
                     /gene="lolF"
                     /gene_functions="other (smcogs) SMCOG1092:hypothetical
                     protein (Score: 345.8; E-value: 7.8e-105)"
                     /gene_kind="other"
                     /note="putative FAD-containing monooxygenase"
                     /product="LolF-1"
                     /protein_id="AAV68702.1"
                     /transl_table=1
                     /translation="MTLTNLDVIVVGAGFSGILAVYRLRKLGFRVQGFERQERLGGVWR
                     ENAYPGAAVDSLFPFYQFYDAELLQDWEWVEQFPTRAEMLRYFDHVDKRWEISASFEFG
                     VSVSAARYSETTQRWTVSLEDGRRAEARWFIPAVGFSSVLNIPRIPGMSRFRGPIYHTA
                     KWPHDAVSMRGKRVAVIGTGPSGVQIIQSVGKIAKAMTIFQQSPCLTLRKYGSPSQTAT
                     ALCMRPDDHREALRLGLQTSNGFGYVPRDQDTLDVPIEERNHFYQQRYLAGGWAFWMAG
                     FRDLCQNIQANRDAYDFWARRTRARISDVAKRELLVPQIPSFAFGIKRPCLEEDLYEVM
                     DQPHVKVIDISNQQIELITETGIRVHGQTVECEAIILATGFGDEASGLRSLHIRGRNGI
                     RLEDAWSDGVESHLGMAIHSFPNMVILYGPQCPTLLVNSPAVITVQVEWLCEIIARCQQ
                     AGICQLEATSKSHCQWERKMSLLWDKTLYHTHARKSKKTAEANKEEKTWVGGLILYRRE
                     LENCLANNLEGFQAWHVEETGLL"
     misc_feature    complement(5033..5074)
                     /allele="1"
                     /gene="lolF"
                     /note="similar to FAD-binding signature sites;
                     pfam00743.11; Region: putative FAD-binding domain"
     mRNA            join(6788..7000,7063..7114,7199..7282,7364..7723,
                     7810..8363,8434..8842)
                     /allele="1"
                     /gene="lolC"
                     /product="LolC-1"
     gene            6788..8842
                     /allele="1"
                     /gene="lolC"
     CDS             join(6903..7000,7063..7114,7199..7282,7364..7723,
                     7810..8363,8434..8707)
                     /NRPS_PKS="Domain: Aminotran_1_2 (83-241). E-value:
                     6.2e-12. Score: 37.1. Matches aSDomain:
                     nrpspksdomains_lolC_Aminotran_1_2.1"
                     /allele="1"
                     /codon_start=1
                     /gene="lolC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DegT_DnrJ_EryC1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1168:O-succinylhomoserine sulfhydrylase (Score: 385.5;
                     E-value: 3.6e-117)"
                     /gene_kind="biosynthetic-additional"
                     /note="putative PLP-containing enzyme; similar to
                     gamma-class PLP-enzymes such as homocysteine
                     synthase/O-acetyl-homoserine thiol lyase"
                     /product="LolC-1"
                     /protein_id="AAV68703.1"
                     /sec_met_domain="DegT_DnrJ_EryC1 (E-value: 8e-09, bitscore:
                     24.4, seeds: 38, tool: rule-based-clusters)"
                     /transl_table=1
                     /translation="MTVDTITSTSNGNQDVPKEFFPKEFETQLLHVGRFPDILGSCAVP
                     VYSSAAFEFNSVAHGARLLNLTQFGNIYSRFTNPTVNVLQNRLAGLEGGVAACAVASGS
                     AAVVVTVMALAGVGDNFVSSFHVHAGTFHQFESLAKQMGIECRFVKSRDPADFAAAIDD
                     KTKFVWLETISNPGNVILDLEAVSMVCHTKGIPLICDNTFGCAGYFCRPINHGVDIVVH
                     SATKWIGGHGTTVGGVIVDGGTFDWGQHPDRFPQFHDPRTRLWERFSRRAFAVRCQFEI
                     LRDTGSTLSAPAAQQLLVGLESLAVRCERHAQNAAKIADWLREHPLVAWVSYVGHPNHP
                     DHQGALKYLKRGFGSVICFGLRGGFEAGALFCDALKMVITTTNLGDAKTLILHPASTTH
                     EHFSSEHRAEAGVTDDMIRLSVGIEQIKDIKADFEQAFEQVLRGKKSLRKPCIGKILLQ
                     DEINEDLFGPSACRT"
     aSDomain        join(7379..7723,7810..7938)
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_lolC_Aminotran_1_2.1"
                     /evalue="6.20E-12"
                     /label="lolC_Aminotran_1_2.1"
                     /locus_tag="lolC"
                     /protein_end="241"
                     /protein_start="83"
                     /score="37.1"
                     /tool="antismash"
                     /translation="LQNRLAGLEGGVAACAVASGSAAVVVTVMALAGVGDNFVSSFHVH
                     AGTFHQFESLAKQMGIECRFVKSRDPADFAAAIDDKTKFVWLETISNPGNVILDLEAVS
                     MVCHTKGIPLICDNTFGCAGYFCRPINHGVDIVVHSATKWIGGHGTTVGGVIVD"
     misc_feature    7867..7911
                     /allele="1"
                     /gene="lolC"
                     /note="Prosite pattern PS00868; Region: putative
                     PLP-binding site"
     mRNA            join(10407..10637,10722..11117,11196..11759,11853..12260)
                     /allele="1"
                     /gene="lolD"
                     /product="LolD-1"
     gene            10407..12260
                     /allele="1"
                     /gene="lolD"
     CDS             join(10460..10637,10722..11117,11196..11759,11853..11977)
                     /allele="1"
                     /codon_start=1
                     /gene="lolD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Orn_DAP_Arg_deC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Orn_Arg_deC_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1264:diaminopimelate decarboxylase (Score: 199.5;
                     E-value: 9.9e-61)"
                     /gene_kind="biosynthetic-additional"
                     /note="putative PLP-containing enzyme; similar to ornithine
                     decarboxylase"
                     /product="LolD-1"
                     /protein_id="AAV68704.1"
                     /sec_met_domain="Orn_DAP_Arg_deC (E-value: 7.2e-19,
                     bitscore: 57.3, seeds: 500, tool: rule-based-clusters)"
                     /sec_met_domain="Orn_Arg_deC_N (E-value: 1.9e-59, bitscore:
                     190.2, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=1
                     /translation="MATVVREAFENHVKLVESRNSPGHVLASSEASFFVADLNDIVRKW
                     AAWKKALPDVTPFFAVKSSYDRRLIQTLATCGAGFDCASVEEIELILSLGIGAERIVFT
                     HPCKPVSSLGLCRKLGITLITFDNECELRKLHHHYPEAQTVLRVFADDPTNADPLGTKF
                     GAAREDIDGLVRLVKELNMKLAGASFHAAPSVAVDAAAYVRGIRDAAEVFARARRVGLN
                     PTVLDIGGGYTDSTFQQIAGAVRPAIAECFKSQVVEGRLRILAEPGTLFSCSPFYLAVK
                     VVARRRNAAAFGNEPATRLYINDGIYSNFMMRFIVNMTFSPVAVIRKGVWYDQTEQTMR
                     REACSLWGRSCDSNDCINRDCRLDPEVGVGDWLVFKDMGAYTTVCNTTFNGFTSSNHTI
                     YIEPTQVDKAQSTFEQLELAI"
     misc_feature    join(10634..10637,10722..10774)
                     /allele="1"
                     /gene="lolD"
                     /note="similar to Orn/Arg decarboxylases; Prosite pattern
                     PS00878; Region: putative PLP attachment site"
     misc_feature    11267..11320
                     /allele="1"
                     /gene="lolD"
                     /note="Prosite pattern PS00879; Region: putative Orn/Arg
                     decarboxylase signature 2 site"
     mRNA            complement(join(12667..13678,13771..14038))
                     /allele="1"
                     /gene="lolO"
                     /product="LolO-1"
     gene            complement(12667..14038)
                     /allele="1"
                     /gene="lolO"
     CDS             complement(join(12782..13678,13771..13962))
                     /allele="1"
                     /codon_start=1
                     /gene="lolO"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) 2OG-FeII_Oxy"
                     /gene_kind="biosynthetic-additional"
                     /note="putative iron-containing oxidoreductase/dioxygenase;
                     contains three conserved amino acid residues (H222 D224
                     H280) implicated in iron binding in isopenicillin N
                     synthetase in SwissProt Accession Number P05326"
                     /product="LolO-1"
                     /protein_id="AAV68705.1"
                     /sec_met_domain="2OG-FeII_Oxy (E-value: 5.6e-15, bitscore:
                     45.1, seeds: 135, tool: rule-based-clusters)"
                     /transl_table=1
                     /translation="MTVTNKPVKPANVPVMDFEAIHASVGNERKKYLRQLDEAWSHHGA
                     IYVINHSIGTRTLEEAFAWCKKFFDLPLAVKNSVHIPPDVSKHFQGWTGTGEAISSQGV
                     WDPDEIERLRKETPTELKEAMELQDPCGTYPPGAPDLNLVEQHLPGFLDFLKKWFAACY
                     KQSLQNMRLVCEILGMEDLDYIGKKFEPRHMSTHSTWNYFLGQPVSQLASGSANRLNAH
                     TDYCQFTMLFQDMVGGLELHDYEEDIYRPVPPIKGAMIVQVGDLLEKQTNGRWRSALHR
                     VTAPSRYMYEGSAGDDDELVQRYSLVFFGHLNLDEMIEPLPGCEKQGKWSTLEWKDRMT
                     AGQWLARRVALEYERKTAATVM"
     mRNA            join(14889..15477,15546..15731)
                     /allele="1"
                     /gene="lolA"
                     /product="LolA-1"
     gene            14889..15731
                     /allele="1"
                     /gene="lolA"
     CDS             join(14952..15477,15546..15649)
                     /allele="1"
                     /codon_start=1
                     /gene="lolA"
                     /note="similar to C-terminal domains of aspartate kinase"
                     /product="LolA-1"
                     /protein_id="AAV68706.1"
                     /transl_table=1
                     /translation="MLDESPMRKGDSVSNDQSNPESNASVSIHQQNQIITCVSPGPVCP
                     NAIEIKRDIVIVRLRPVESCPGYRFFRRVFETLEKWQLQVDMFSTSLGRITLALGAAAL
                     QAGISDSCSARNDMMSRDLMHGMQKLLPDDHIELFPHMAIISVVGHPSRRMAGHIFATM
                     DANDIPTVMISHDAARLGIACAISEQYTAKALCVFEQCLFRYSLTH"
     mRNA            complement(15759..17493)
                     /allele="1"
                     /gene="lolU"
                     /product="LolU-1"
     gene            complement(15759..17493)
                     /allele="1"
                     /gene="lolU"
     CDS             complement(15890..17377)
                     /allele="1"
                     /codon_start=1
                     /gene="lolU"
                     /product="LolU-1"
                     /protein_id="AAV68707.1"
                     /transl_table=1
                     /translation="MASPGNHAIVAPGWTRSEDGSLTRPLDLVENWLLARIQRANTPPG
                     REAEGLTYKLKLRLPQDIDDPIPYLRRAWLVFRYVQPLIGAIYPPYSERDETGRYLVTV
                     PLMDPEEWLRLSFHVNQGTQAVFRDVDDAGKIFQPRPTAMAYWFPPSSTLIIRSTHLRF
                     DAVGIYKATNTFMLGLESVFRLGLDANLDCYTTDVKQPSLPPGIDYILGFPPQETPVPH
                     RVERAVDELMRHWHHGLYSLSLPVREGSEDAAPANTQHLVTLFDEPTLEAIVAGCKKLG
                     VSVSAAVHASIVRVWASFPQQQHTGARNMLIPLVANLRPLLDPKWVVPDYALGLCIFVV
                     PFCLTGGFEDLTQRLGAVYSRDLSALPSDPAGDPVSFLELLPLYESREAAFLGSLPVAG
                     CPPFRVPNLSSLGVLERYLARAYGKKGAQAPVCEIEDVALVNATTDPTIEFQLFTFRGT
                     MRLYLYYNDAYYTEDFLAPVMEMVRDSLLQELGLGGS"
     misc_feature    complement(17312..17338)
                     /allele="1"
                     /gene="lolU"
                     /note="Prosite pattern PS00037; Region: putative Myb
                     DNA-binding domain"
     mRNA            join(<19241..19557,19631..20694,20818..20876,20990..21230)
                     /allele="1"
                     /gene="lolP"
                     /product="LolP-1"
     gene            <19241..21230
                     /allele="1"
                     /gene="lolP"
     CDS             join(19245..19557,19631..20694,20818..20876,20990..21044)
                     /allele="1"
                     /codon_start=1
                     /gene="lolP"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1034:cytochrome P450 (Score: 206; E-value: 1.7e-62)"
                     /gene_kind="biosynthetic-additional"
                     /note="cytochrome P450 monooxygenase"
                     /product="LolP-1"
                     /protein_id="AAV68708.1"
                     /sec_met_domain="p450 (E-value: 1e-46, bitscore: 148.8,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=1
                     /translation="MDLTQFNTAGIVWPTVAAIAISYILLSSFLSWYRLRHIPGPFLAS
                     ISSLWNVLNIVTGRTSPVLEKLPGKYGPLVRTGPNYVLTDDAEILRHVNGARSTYPRNG
                     WYEGFKVDEHDHMGSHIDTSVHDAIKSKVIGGYNGKDGIDLEGAIGSQVKTLVSEIRRR
                     HLGQPVDFSRLMRQMALDAITAVAFGEALGFLTAEDGDVFGYVSAVDKMLTYLTLASDL
                     PVVRSVVRSRRMAPAVRCVLAYTGIGRMLNHTRRVVAERYAADDPGKGDMTASFIRKGL
                     TQIECEGESHLQLIAGADTAVTVLRSTLLYIMTTPRVYTRLKAEIKAAVDAGEVVEVIT
                     MAQAQRLPYLQAVVLEGFRMRPAVVYGHFKSVPAGGDTLPNGVRLPAGTAIAPNYIALT
                     RRADVYGADVDLFRPERFLDAEPAKRHEMERAMDLNFGLGRWQCAGRNIALMEMNKVFF
                     ELLRHFDLQILYPGKAWDEYTGVVYSQHNMWVQITESS"
     misc_feature    20623..20652
                     /allele="1"
                     /gene="lolP"
                     /note="Prosite pattern PS00086; Region: putative cytochrome
                     P450 cysteine heme-iron ligand signature site"
     mRNA            complement(join(<21485..22169,22245..22836,22914..>23001))
                     /allele="1"
                     /gene="lolT"
                     /product="LolT-1"
     gene            complement(<21485..>23001)
                     /allele="1"
                     /gene="lolT"
     CDS             complement(join(21485..22169,22245..22836,22914..23001))
                     /NRPS_PKS="Domain: Aminotran_5 (25-251). E-value: 2.6e-19.
                     Score: 61.2. Matches aSDomain:
                     nrpspksdomains_lolT_Aminotran_5.1"
                     /allele="1"
                     /codon_start=1
                     /gene="lolT"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139:aminotransferase class V (Score: 99.9; E-value:
                     2.4e-30)"
                     /gene_kind="biosynthetic-additional"
                     /note="putative class 5-aminotransferase; coding region,
                     intron and exon positions deduced from lolT-2, encoded by
                     GenBank Accession Number AY723750"
                     /product="LolT-1"
                     /protein_id="AAV68709.1"
                     /transl_table=1
                     /translation="MTVNSKRIPFGKPMLEAFCMDPEYTNLNSSSCGSWPKVVSKQIRD
                     YWSLLEAQPDLFSEFSQGLVLQEARIGLAHLVHAAVSECVLVSNVTTGIFTVLYNQAFE
                     ERDVVVTLSTTYGAIDHGITSLAETRPFKTRRVEFELPTTGQKIVSQFETAMAQIRADG
                     LRPRLAILETIVSIPAVRMPFEDLLRVCQKEGIMTLVDGAHSVGQFEVNLQELQPDFFV
                     SDCHKWLFVPRPCAVLYVAERNQHMMRSVIPTSFGFIPKNGNSRLPLWSQMVSASETAS
                     SFETLFAYTATSDYMPHLCIPAALRFRRDVCGGEAAIYEYIKWLAKEGGDKIADILQTE
                     VLEEPGLGAGVDGQMRNCGIVTVRLPLAIATGPSTAPAHVPGGALTEKEVGPAVRYLTK
                     ALAERYKTWIPIIDYRGWIWARLCAQVYLEVSDFEMAGNSLKVICEEILNREMGQ"
     aSDomain        complement(join(22097..22169,22245..22836,22914..22926))
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_lolT_Aminotran_5.1"
                     /evalue="2.60E-19"
                     /label="lolT_Aminotran_5.1"
                     /locus_tag="lolT"
                     /protein_end="251"
                     /protein_start="25"
                     /score="61.2"
                     /tool="antismash"
                     /translation="NLNSSSCGSWPKVVSKQIRDYWSLLEAQPDLFSEFSQGLVLQEAR
                     IGLAHLVHAAVSECVLVSNVTTGIFTVLYNQAFEERDVVVTLSTTYGAIDHGITSLAET
                     RPFKTRRVEFELPTTGQKIVSQFETAMAQIRADGLRPRLAILETIVSIPAVRMPFEDLL
                     RVCQKEGIMTLVDGAHSVGQFEVNLQELQPDFFVSDCHKWLFVPRPCAVLYVAERNQHM
                     MRSV"
     misc_feature    complement(join(22139..22169,22245..22273))
                     /allele="1"
                     /gene="lolT"
                     /note="Prosite pattern PS00595; Region: putative
                     aminotransferase class V pyridoxal-phosphate attachment
                     site"
     mRNA            join(<23453..24193,24273..>24304)
                     /allele="1"
                     /gene="lolE"
                     /product="LolE-1"
     gene            <23453..>24304
                     /allele="1"
                     /gene="lolE"
     CDS             join(23455..24193,24273..24304)
                     /allele="1"
                     /codon_start=1
                     /gene="lolE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /note="putative epoxidase/hydroxylase"
                     /product="LolE-1"
                     /protein_id="AAV68710.1"
                     /sec_met_domain="PhyH (E-value: 9.8e-26, bitscore: 80.5,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=1
                     /translation="MTAASSPHPGVSAEDIEFYQANGYLRLPQEAHGLFDDLAKLQAWV
                     AEISQWGLETGKWRHYYETTNGKHLLWGTEKLMEYHAPMRDLIAGEAPLTLLKSLTGKD
                     MVVFKDEIGWKLPGGKGAVPHLDRPAYSMFAPEFIEIMIAVDAHTVENGCLQFVPGSHK
                     EAVPISADGRIASAWLEGKEFIPMVLDPGDVLIFNESMAHRLDPNKTDQRRAAVFGTYH
                     FDRSQPDLRDKFYAHRLIHSPPENAWVETVEAQT"
ORIGIN
        1 atatttatat taaatagtgc ttattatcta gattaaataa tcctatacta tttatctaat
       61 agttttatta ctaatattag ggtatatagc tatacttttt cttaaggagt tttactatat
      121 taatttcctt tcttacttag gctataacta taatttttag tttagtaagt tactttatta
      181 gattatttat ttaattatag tattttacta aattaggagc tatctactac cctagactag
      241 tgttaaataa aatatttttt ctattctaac taggtaaatt actaaatatt atctaatttc
      301 tctaagtagc ttagtaacta ttaaaggtag cttaattaaa tagtgcctat atcttaaagc
      361 taaattctac cttatctaaa gtaatttttc ctaagttaaa gaagttagga tccctattct
      421 taagctctaa ctatatataa atatatatgt taagtgctat ctaaagctat ttatattata
      481 atactagaat tttctcttaa gtaaaccctt tttaatttat aaagcttaag attaaatact
      541 taggaagtgc tttataaata taattagact aggttatagt agttctctat tctattaggt
      601 tagccttaga taataaagta taatacttct atagctagta ataggtttcc ttaattattt
      661 ttagttatat aaaattaagg tctttaagtt atcccctagt gttttctatt taaccctaat
      721 tagtaatatt aaactaaagg agtatcttat ccttagactc attttaagtt tataatatag
      781 ttagtatttt aactacttat taaagcttta agttaatatc ttacttaagt atcttacact
      841 agtttaactt aggtatacct attaccttta tctttacttt aaaatcttta ataaagttag
      901 taatattatc ccctataaac tactaaatat tgatttttta aattttttta ggggttatta
      961 gttatagcca atgcatctaa taaaataaga taataataat tactagcaat acctagctag
     1021 tataataatt aacttataac taatcttaac tatagtaata aataaaaccc taatagaaac
     1081 aagctttaag gttataaata tagggggggc tataaggtta gattaaagtt tgtaataaac
     1141 tttatagcta cgttagcgca agatatagtc atagttcgcg accgcagtca aactcaaatt
     1201 cgttcgataa actactaaat attataccta ttttatatag taataaggga tatctaatac
     1261 actattattt ctctaagaag ctttataaag gtctttatat taaggcccct aaggggttta
     1321 ggtatagtct ttctattatt aggatctttt cctaagtagt ttttaaatta ggtttatact
     1381 aaactagtaa ctattttaga attaattaat ttctaactag gaactagagt actaggtaat
     1441 agaaattctt agagatcctt taggtaactt agctacttac taggccttac tttacttagt
     1501 tatttaaaat aaaataaggt aaggttaaat atagaattag ctaagagtgc ttttaattaa
     1561 tctattagaa aagtaatagc ttaactaaat atctaaatac ttataagact ttactaaaaa
     1621 agggttttta gtagtgtaat atttatagtt ataagtaagc tataagtaaa ggctattgat
     1681 tttttaaaat tttttggggg ttattagtta tagctaatgc atctaataaa ataagataac
     1741 tataattact agtaatacct agctagtata ataattaact tataactaat cttaactata
     1801 gtaataaata aaaccctaat agaaataagc tttaaggtta taaatatagg gggggctata
     1861 aggttagatt aaagtttgta ataaagtttg tggctgcgtt agcgcaagat atagtcatag
     1921 tttgcgaccg tagttaaact caaattcgtt cgagttttta agtaaaggct ataagtataa
     1981 ttatatagct ctttataata agctataaga ttctaatact tagctaaata actataaaat
     2041 taataataag tcttaagatt aaatagttta taacactagg tagcgcttcc tatcttaggt
     2101 tcttatattt agagatctct agtaaggggt taatagctta gctagtgttt atagaatata
     2161 ccttaggatt agttactatc ttcctctata gttttatata atataatttc ctcttcttaa
     2221 tctatcttaa ctctctaagc ctagctagcc ttatattaat actatagcct cctttaggta
     2281 taagcctagg ctattaattt atattatact atagggaaaa taaaataaag gggttttata
     2341 taaaatagct tagtaatagc taaagaattt agctagaaat acccttctaa ttaatcttag
     2401 ctagaagtaa gttaattaga gagtctaagg tccttagggt tttaaaacct atttctctac
     2461 tagttttatt aagtaaaaaa taatacccta aaagacataa tactagccta tagttatagg
     2521 tagtaatagc atttacttac actaaagggg ttaattaact taatagtagc aattataata
     2581 atagctaaat ctatctctat atatagggat agattagatt agattaaata atctattaga
     2641 ttagaaatgc ctttttaatt aaatcagggg aaaatagtct tattaattta tcttattaaa
     2701 gtaaatataa tagtagctat agtaacacta ggccttaaag tcaaaaatct agagttagtt
     2761 atatataatt tatactaaag aaatctagct ataggtagtc cctttataga ttaaataatc
     2821 cccttatcta gataactaat atcgttatcc cttaatgtgt tatccattcc tttaatccct
     2881 agtgcttatc atcacgatcg cgtgttttgt gggcgcgcgt atcatagcac acagtacttc
     2941 ttctagattt ggactaacgt ggtatgcgca tttagtggcc ctaggacgta tggtgccgga
     3001 ctgttgtagg ctatccgtcc tagcctaacc tatctaggca ttggtctagc aacaggctta
     3061 cgggggctag ctaagatgta gatatgtaca agatgctatc aaactgctat tgtgtttgcc
     3121 gagaaggtta ctagcagatg ccaccgacgg aaacgatgcc cattttcttc tggcacagaa
     3181 tcagagacgt attgggtgtt tgtcaatacc agagtctgat cagtggaaca tggaaaatcg
     3241 gggggacaaa atctgcctag ccgtcatcca aaaaaaaaaa aaccaattgc tacttgcgtc
     3301 tagacctgaa gagtgtatcg actcgatata cgacagaaag tggcttcaca gaagacccgt
     3361 ctcctctaca tgccacgctt gaaacccctc caggttgttg gccagacagt tttccagctc
     3421 ccgacgatac agaatcaacc ccccaaccct gatcagtttg ttagagcagt ccacagaaca
     3481 aagcgacaag agcccttgtg caacctacca agttttctct tccttgttag cctcagctgt
     3541 tttcttgctt ttgcgtgcat gtgtatggta gagtgtcttg tcccaaagta ggctcatctt
     3601 cctctcccac tggcagtggg atttggaagt cgcctcgagt tgacaaatgc ccgcctgttg
     3661 gcaccttgcg atgatttcgc acaaccactc tacctgaacg gtgatgaccg cgggggagtt
     3721 gaccagaagt gtagggcact ggggtccata gaggatgacc atgttgggga atgaatgaat
     3781 ggccattccg agatgcgact cgacaccatc ggaccaggca tcttctaaac ggatgccatt
     3841 ccggcctctg atatgaagac tcctgagccc gcttgcctcg tcgccaaacc cggtggcaag
     3901 aattatggct tcgcattcaa ctgtctgccc atgaacgcgg atacctgtct ctgtaattaa
     3961 ctcgatttgc tggttgctga tgtcaataac cttcacatgg ggttggtcca taacctcgta
     4021 aagatcctct tccaaacagg gtcgcttgat accaaaggca aaagatggaa tttgaggcac
     4081 caggagctcc cgtttggcca catcgctgat tctagctcgc gtccgccgag cccagaaatc
     4141 atacgcatcc cggttggctt ggatgttctg gcacagatcc cggaacccag ccatccagaa
     4201 agcccagcct cccgccagat agcgttgttg ataaaaatgg tttcgctcct ctattgggac
     4261 atccaacgtg tcctggtcac ggggcacgta gccgaaaccg tttgaagtct gcagtccaag
     4321 tcgtagggct tctctgtggt cgtcgggtct catgcaaagt gccgttgccg tctggctcgg
     4381 gctgccgtat ttgcgtagag tgaggcatgg agactgctgg aatatcgtca tggccttggc
     4441 tatcttaccc acagattgga tgatctgaac tccgcttggc cccgttccaa tgacagccac
     4501 cctcttaccg cgcatactga cagcgtcatg aggccatttt gcggtgtggt agatgggacc
     4561 gcggaatcga gacattcctg gaatcctggg gatgttgaga acggacgaaa accctacagc
     4621 tggaatgaac catcgcgcct cggctcttct gccatcttct aaagagacgg tccatctctg
     4681 ggtagtttct gagtaccggg ccgcggaaac cgaaacgcca aactcaaaac tagcagagat
     4741 ttcccatcgc ttgtccacgt ggtcaaaata tctcagcatc tctgcacggg tgggaaattg
     4801 ctctacccat tcccaatctt ggagaagctc cgcatcatag aactggtaga agggaaacag
     4861 gctgtcgact gctgctcccg gataagcgtt ctcgcgccag acacctccaa gacgttcctg
     4921 gcgctcaaac ccttggactc gaaatccgag ctttcgtagt ctgtagtcat agttcagtcc
     4981 tacctctcta ctctattcgt gtcttggatg gcaacgtaga ctctcacctg tagacagcga
     5041 gaatacctga aaaaccagcg ccaacgacga ttacgtccaa attggtcaat gtcatcttag
     5101 tgaaatgaag aagaattaaa taatcatatg cagaatagaa gcaaattttg gtgtatcaga
     5161 aatgtcatca aaggctaaca gctccgggga cagaaaagct tgatcgaggc tgcaatttac
     5221 tgagaggcgt aagggactga tactaagacg gtcgattatc tgtgtgcgag tggtcagaat
     5281 gggtaactgc gcatttggat agcccagttt gggaggggga gtatagtcgt acacatggat
     5341 tcgcaatttg ggaatgaagc gttaagtctg ttatggtata atagggtcac taaatgcaca
     5401 taacgcgtta gtcctaatcc ggattaacga tccatgcacc ttatgcctat atgtgtcata
     5461 tttaaaggta ttgatattat taaagctatt acaattacca ctatactagt attaacttaa
     5521 gtaattaact ctctattata tacgccttaa ataattacta ctaaataaat tcaaccacta
     5581 taaatccgtt acttactaag tattaatagc actgattata tcttttatag ttatatacaa
     5641 tatctttatc tttaataatg acataataaa gggcacttaa aaagagcctt attatattat
     5701 attgcgttgc attgcattgc gttacattat attagaaggc tttttacaat aagcattata
     5761 gtaagccact tgcattttat actagctctc taatatactt agcaagggat taagtaaaca
     5821 tgtacttttt ttgttttagg actaacgcgg tatgcgcatt tagtgacccg gtataatagg
     5881 gcataggaga ggcgtgagtg gggtgttcac tcactccgtg tcacgtcact tctcacaaag
     5941 gagagggagc gtggctattc ggacctgcgt tttcttgctt actagagctg tgcctaccac
     6001 gtaatagggc cacatcccac atcccacgcc ccgccactaa attatttgta tagcactccc
     6061 tagcaataaa aatagtcaga attacagtac acccctgagt tattttacag atctacataa
     6121 caggggggat tgagcgaatt aagtgctagc aaagaaataa gggagatagg ggctaggatt
     6181 agcaagtacc tagattcctt tatactttat atagaaaatt atatatcttt tagtagtgct
     6241 atatatgcta tatattttcc ttatagctct ctaaaataat ataggggttc tagtgcactt
     6301 atagctaatt atagctatta gagaggaacg aaaaactagg gcatgtagat ttgtaaaatg
     6361 aatgtgtaca ggcctgtcgg ccccgcccgc cgcaataggt aatagctgta ctacgcccgt
     6421 agtggccacc cacttatcat gccacgcccc acttcttttg gttcgcgtgc cacttcagtg
     6481 gttattactc cgtattcata agcttgacaa acacacatcc gcgacaacgg tatcctcatc
     6541 tgcagggccg cataccataa tgcttgctgc agctcattga taatatacct ggcctaagat
     6601 agacacgacc gaccggctgg agtcatcggc tttgcctggc cctccgctat tttgagagac
     6661 aagatcggcc tggtgactac aatttgctac caagacagat tcgctgaata tccgcaagca
     6721 agcaagctgc atcatcaaaa cataaataac gaccaaagaa tacacggagt agaagaaagg
     6781 aaggaccaga acaagttggc gattttccaa cttggttact cacccaagcc gtcactctgt
     6841 tcatatcatt actcgccttc cagtgtcttg cccaccttgt ttcaatattg tcgtcgagca
     6901 aaatgacagt agatacgatt acttcgactt ctaacgggaa ccaagatgtt ccaaaggagt
     6961 tcttcccaaa agaattcgaa actcagcttc tccatgttgg gtaggttatc gcgcctcata
     7021 tgacggtcct ctatcccaga actaatcagt ttgcgattca agccggttcc cggacatttt
     7081 aggcagttgc gcggtgcctg tatacagttc ggcagtaaga tcaagccacc gcctcctcga
     7141 aacccaccaa ctatgccaca tgtactgaat tcttttccct ttttgcgcat cttgataggc
     7201 ctttgagttc aacagcgttg cccacggtgc gcgtcttcta aacttgacgc agttcggcaa
     7261 catctacagc cgcttcacca atgtttgtct ctttctcctt ctccctcatg actgcttttt
     7321 taacgaggac acaagctaat aaaacaaaac catccttcaa cagcccaccg tcaatgtatt
     7381 gcaaaatcga ctggccgggc tggaaggagg cgtcgctgct tgtgccgtcg catccggctc
     7441 tgcggcggta gtcgtgacgg taatggccct cgcaggcgtt ggcgacaact tcgtctcatc
     7501 ctttcacgtt catgctggca ctttccacca gttcgagagt ttagccaagc agatgggcat
     7561 cgagtgccgc tttgtgaagt ctcgagaccc tgcagacttt gcggcggcca tcgacgacaa
     7621 gaccaagttc gtctggcttg agaccatcag caaccctggc aacgtaatac tagaccttga
     7681 ggcagtctcg atggtctgcc acaccaaggg cattcctttg attgttagta tcccaatgaa
     7741 aactgtccgt cccatagggg gggttggggc taaaattcgg ggggatgtgg ttcccatcca
     7801 agattttagt gcgataacac ctttggctgt gccgggtact tttgtcgtcc catcaaccac
     7861 ggcgtcgata tcgtcgttca ctcggccacc aagtggatcg gcggccacgg cactacggta
     7921 ggcggtgtca tcgtcgacgg cggtaccttt gactggggcc agcacccgga tcggttcccc
     7981 cagttccatg atccacggac gcgactctgg gaacgctttt cccgtcgggc gtttgctgtc
     8041 cgctgccagt ttgagatcct gcgcgatacg gggagcaccc tcagcgctcc tgcggcccag
     8101 cagctgctgg ttggcctcga atcccttgcc gtgcgctgcg agcgccacgc gcagaatgcg
     8161 gccaagattg ccgactggct gcgtgagcat cccctcgtgg cctgggtcag ctatgttggt
     8221 cacccgaacc accccgatca ccagggagcg ctcaagtacc tcaagcgagg ctttggctcg
     8281 gtcatctgct ttggtctacg ggggggtttc gaagcaggtg ccctgttctg cgatgcgttg
     8341 aagatggtca tcaccactac caagtgcgta attaaactag ccccttcttc tcccgagaga
     8401 gcatcgttct gccattctaa cctcgctttg cagcctgggg gatgccaaga ccctgatcct
     8461 ccatcccgcc tcgactactc atgagcactt cagttccgag catcgagctg aggctggcgt
     8521 cacagatgat atgatcaggc tgtctgtggg tattgagcag atcaaggata tcaaggccga
     8581 cttcgagcag gcctttgagc aagtgcttcg gggtaagaag agtcttcgta agccttgcat
     8641 cggaaagatt ctcttgcagg atgagatcaa tgaagactta tttggacctt cagcttgtcg
     8701 tacgtaaata ggggtcattg cgaggcatgg taaatgttct accagaagtg gcatggaagt
     8761 attttccaat agacctgtaa ttcacggttg ctacgttttt tcatacttac catgcggatt
     8821 tattttgata atatttcttt aagtgtgcag aactcgtagt cacttacaaa gctaattgcc
     8881 gtaattaatc tttagtgcgc catgtcctct tgactatctt gtaaacgaag gggcattagg
     8941 cgtgctttgc tttgtctctc ctcgtgtttt taagttttgc tatattaact tgaatattat
     9001 tactaactag atgctaggct ttttactatt tacaaacacc tgtaacaatc agcgcggcgt
     9061 taggcaactg cagagattat tttactatag tatagactat aatgccatac tttaattaat
     9121 cttcagtacg ctatgttttt ttcactatct tgtacagatt aaacaaaggg ccattaggca
     9181 tgtgttggga gcataaaggc aaagaggtca gagacaggac aaccgtatag tgaatccttg
     9241 gatgactaag cgtagttgcc tagctagtag atctccttaa gcgtgctaca gcttgtcaag
     9301 tcacgttagc tagagtctat atctaattag ataaaagtac attaaaatag tatatatatt
     9361 tatatgtaaa ttagctaata atcgttatac tagtaatttt ctatagtata gattactata
     9421 ttacactcat taattatctt agataagtag tacgtactta cgcggttatt aaccttatag
     9481 cagggggggc tgacgtaagg tagcttagga gtataagggg gctgtaaggg ataatccttt
     9541 cttctctcct ccagtttctt aatactacat cgacgcccta tcgcagtcgc ctaggggcta
     9601 gtctataaca ggaggttaga gtttgagaca tttacgcaca aacctaatat aggtagcctt
     9661 gttattagta acaaagaatc ttagctatta ctcggtttta acaaaccagg ccattagcca
     9721 cggcagcatg ctccacttag tctctcccag tgttttagag ttatactatg ttattacata
     9781 aaatacatat actattattt taaagtaaac tttcacattt taaaacttat tatgttaaaa
     9841 gtggataatt catgcatata ttaacttgaa tactattact tactactcga tactaggctt
     9901 tttactattt acaaacaccc ataataggcg gcgtggcgtg aggcaattga ggagacagtc
     9961 tcacaatagc agggagcata tcacattcat ttatagtaca gatgccgagc caaatgccgt
    10021 gcgtgatcga aacggtgagc caaagcgcag cacaccatca agatgcggct gtaatctcgg
    10081 ttgtcacttg gaagcacttg gaagcactcg tgcataacgc aagtcgctct ctcatacatg
    10141 gctttcagag tttatgaggg actagaacag acaagtctgg ctttttgaca aggaagtagc
    10201 tggacaaggt tcgcaggcgc tctgaccgag cataaaccaa tattggagac tggagactct
    10261 gccatgtctt caataggccc ttatgtagcc ttgcttgtgt gtgtacttgc agggatgttt
    10321 ataaatatgt gtatgttcga gtatcttgtt cggttttgta aactcaaact cgccagctct
    10381 cagcctgtca gccgagtgaa ttgctattgc cattgtatta ctccagaaca cgtatcttgc
    10441 acaaactcca atctcagcaa tggccaccgt cgtacgagaa gcatttgaga accatgtcaa
    10501 gctggtcgag agtcggaact cgcccggcca tgtgcttgct tcttctgagg cctccttctt
    10561 tgttgccgac ttgaacgaca ttgttcgtaa gtgggcggcg tggaagaaag ctctcccgga
    10621 tgtcacccct ttttttggta cgtcctcaat tccccccatt tcatgtctca ctaagctggg
    10681 aagtcccgtc cgagggtccg aatcaattga cttggaggca gccgtgaaaa gcagctacga
    10741 tcgacggctg atccagactc tggccacctg tggagccgga tttgactgtg cctcggtgga
    10801 ggagattgag ttgatcctct ccttgggcat tggcgcagaa cgaatcgtct tcactcatcc
    10861 gtgcaagccc gtctcctccc tcgggctttg ccgcaagctt gggatcacgc tcatcacttt
    10921 tgacaacgaa tgcgagctcc gtaagctcca ccatcactat cccgaggctc agaccgtcct
    10981 ccgcgtcttc gccgacgacc caaccaatgc cgatcctttg ggtaccaagt ttggcgccgc
    11041 gcgggaggac attgatggac tcgtgcgtct ggtcaaggag ttgaacatga agctggccgg
    11101 cgccagcttt catgcaggtg cgtctcgctc tcggtacctc ggccggtgaa atgcatgcag
    11161 ctcgctaacg gcggcaattc cacgccctgt tctagcccct agcgtcgctg tcgacgcagc
    11221 tgcatacgtg cggggcatcc gggacgcagc cgaggtcttc gcgcgggccc gacgggtcgg
    11281 gttgaaccct acggtgctgg atattggcgg cggctacaca gactcgacgt ttcaacagat
    11341 tgcaggggcg gtcaggccgg ccatcgccga gtgcttcaag tcgcaagtgg tcgagggacg
    11401 ccttcgcatc cttgcggagc cggggactct cttctcctgc agcccgttct atctagcagt
    11461 caaggttgtc gcgcggagga ggaacgccgc tgcgtttggg aatgagccag ccacgcgtct
    11521 ctacatcaac gacggcatct acagcaactt catgatgcgt ttcatcgtca acatgacctt
    11581 ctcgcccgtg gccgtcatcc ggaagggggt gtggtacgat cagacggagc aaacgatgcg
    11641 ccgcgaggcg tgctctcttt ggggccggag ctgtgactcc aacgactgca tcaacaggga
    11701 ttgccggctc gatccggaag tgggggtcgg ggactggctt gtcttcaaag acatgggtgg
    11761 tgagcgtctc aacttttccc cccttttcct tttcgttttt gggtccctgt ggagaatagc
    11821 atatgtagga gctaaccatg catggtgggc agcctacaca acggtatgta acaccacctt
    11881 caatggcttc accagttcca atcacacaat ctacattgaa cccacccaag tcgacaaagc
    11941 ccagtcgacc tttgaacagt tggagttggc catctgagtg ccagttcggg gagacccaca
    12001 cgcggcacgt gccgtcccgg gttcccgggc gtggctgcat gacgctagac gcgctagtct
    12061 agtacctact ccgttccgta ctgtccttgc agcagtccgt agtcacaaca gatggcttgg
    12121 atcaattgat gcacactccc tgattagctt gtttgacaca ttccatttgg cttcgtgcac
    12181 atcatgatac aaccagtaca tgtttcctcc agtccttttg tacatggcca cgccgagctc
    12241 ttgtaagtac ctcgtcgagc ttggtcttcc tgcaccaagt tatgtacagt gggggtgcag
    12301 atgtaaggtg cagatgtaag gataaccaca aaaattagtc ctgttaggca aaatgacctt
    12361 ataatttaaa taaatagcta aataaaatat ataatatata attatactaa gaagttattt
    12421 ctcttttacc tatattactt aaaactttat attaaaccct ttagcgtgtg aaataaatta
    12481 agttaaacct aagataaaat ccttcattat cctcacatct gcacccccac tctaggtgcc
    12541 gcgctcccgg agaaggggtc caccccaagt caccccgaga acggtaaaaa tgacgacata
    12601 tggcggaacc cccttctctt agcgacaatc gcaatgagac ggacaaggta caacgacctg
    12661 agtttctgac cgactggcat aagtgctcat gattgaatac taggtagcag actgtacgga
    12721 ctagttcgtt gtttcagtgc aaatcacttc actactacaa gcctcaggcc tccgtcgcct
    12781 cctacatgac cgttgctgct gtcttgcgct catactcaag agcaactcgg cgggccagcc
    12841 actgccctgc cgtcatccgg tccttccact cgagcgtgct ccactttcct tgcttctcgc
    12901 agccaggcag aggttcgatc atctcgtcca gattcaagtg tccgaagaag acgagcgagt
    12961 agcgctgcac cagctcgtca tcgtcaccgg cacttccttc gtacatgtac cggctcggcg
    13021 ccgtgacgcg gtggagggcg ctccgccatc tgccgttggt ctgcttctca agcaggtccc
    13081 caacttgaac aatcatagcc cccttgatcg gaggcacagg tcggtaaatg tcttcctcgt
    13141 agtcgtgcag ctcaagcccc ccgaccatgt cctggaagag catggtgaac tggcagtagt
    13201 ccgtatgcgc gttgagccgg ttcgcggatc cgctggccag ctgtgaaacg ggctgcccca
    13261 ggaagtagtt ccaagttgag tgggtgctca tgtggcgcgg ctcaaacttt tttccaatgt
    13321 aatccagatc ctccatcccg aggatttcgc acacgagccg catgttctgg agagattgct
    13381 tgtagcaggc cgcgaaccac ttcttcaaga agtcgagaaa gcccgggaga tgctgctcca
    13441 ccaagtttag atcgggggcg cccggggggt acgttccgca agggtcctgc agctccatgg
    13501 cctctttgag ctccgtcggc gtctccttgc ggagcctctc gatctcgtcg ggatcccaga
    13561 cgccctgcga ggagatggcc tcgcctgtgc ccgtccagcc ctggaaatgc tttgatacgt
    13621 caggtgggat gtgaaccgag ttcttgaccg ccagaggcag gtcaaaaaac ttcttgcact
    13681 gcatttcaaa aggcacacaa gatcaattag cgatcctaac ttgcatgggg aatgccagcc
    13741 cattcttgag taaccaggcc agctacttac ccaggcgaat gcttcctcga gcgtcctagt
    13801 tccaatactg tggttaataa cgtagatggc tccgtgatgg ctccatgcct cgtcgagctg
    13861 tcgcaagtat ttcttgcgct cattcccgac actggcatgg attgcctcaa agtccatcac
    13921 tggcacatta gcaggcttaa caggcttgtt tgttaccgtc atcttgggag ttgaacagat
    13981 agaatcttga tgttgaattt gggattagtg cctcggaaag agtgaggaat cgtgatatgt
    14041 ggagcggatc gttaaataaa ggctttgagg aggtcattta taatagtgtc aacaagctga
    14101 tttatcacac tgcaatcctg catctatatg acacggtctc tattattgga ggggtctctt
    14161 aatgcgcata ccgcattagt cctggaacaa aaggagtacc tttccactta atcccttact
    14221 aaatatacta gccatcttta gtagtgaata taagtggctt aatataatgc ttatagcctc
    14281 ttctatagaa aattgctttc tttttactat tatcaaagcc ttataatata acgtaatata
    14341 acgcaataac gtaacgtaac gcaatgtaac gcaatataac gtaatgcaat aaggctcttt
    14401 ttaaatactc cttattatat tattctttaa gatagctatt gtatataact atagaaaatt
    14461 gaattcatat tattaatact tagtaagtca gtgaggggtt aatagcaaaa taaattcata
    14521 ataattcttc aaggtatata taataaagac ttaattattt aatttaatac tagtatagta
    14581 gtaattataa tagctttaat aatattaata ccttttaata tcttatatgt aagcatcttc
    14641 gttaatagtc aaaatcaata gacgcaagcc acactaaagt taggtgtgta ggactaacgc
    14701 tatatgcgca tttagtgacc ttaatcaacg gtttcaaagt ggtatgatct cattagattc
    14761 tcggggatcc tcttgaggga cacgatacaa atcttcttga gctacgtttg ccatcgttat
    14821 gagaagtggc tgacaagtac ttcgtaattt aagagcatcg cgtctggcga attctacaga
    14881 cacggaacgt cagtgacacc aaacccattc agcccaccca caaaatctca attcgactgt
    14941 attttctaac gatgctcgat gaaagcccaa tgaggaaggg tgattctgtg agcaacgacc
    15001 aaagcaaccc ggagagcaat gcatctgttt caatccatca gcagaatcaa atcatcacct
    15061 gtgtctcccc aggcccagtc tgccccaatg cgatagaaat caagcgtgac attgtgatcg
    15121 tgagactacg gcccgtcgaa agttgtccag gctatcgctt ctttcgccga gtctttgaga
    15181 cactagagaa atggcagctg caagtcgaca tgttctcaac cagtctgggg cgaataacct
    15241 tggcgctggg ggccgcagcg ctgcaagccg gcattagtga ctcatgcagt gccagaaatg
    15301 acatgatgag ccgggatctc atgcacggca tgcagaagct actcccggac gatcacatag
    15361 agctctttcc tcacatggcc atcatctcgg tcgttggaca cccgagccga cgaatggccg
    15421 gccacatctt cgccaccatg gatgccaatg atattcccac ggtcatgatt tcgcacggta
    15481 tggcctcaat cctttcccgt ttccatagca aatcatcgtc ccattcttct gaccagcatg
    15541 ttcagacgcc gccaggctcg gtatagcctg tgccatctcg gagcagtaca ctgctaaagc
    15601 cttgtgcgtc ttcgagcaat gcttattccg gtactccttg acacattgaa ccgttatctc
    15661 agtctcttca tcaaaactct tctcgttgca gaattagata gattagatgc acaagacact
    15721 cgttgtgggc tatggttttt ttgccgtgat agacagctaa aactaaaacc tcattgtagt
    15781 tacaagcagc tttagtactt cccgtgatac tgacttttct ttagcagctg atcgaccact
    15841 ctagtgacaa caacgttcaa gattccttcg gatctagtgc ttctgaagac taacttccac
    15901 cgagtcctag ctcctggaga agactgtcgc gcaccatctc catgacggga gccagaaagt
    15961 cttccgtata gtatgcatca ttgtagtaca agtacagccg catagtgccg cggaaggtaa
    16021 atagctggaa ctcgatggtt ggatcagtcg ttgcattgac gagggctacg tcttcgatct
    16081 cacaaacagg ggcttgtgcc ccctttttcc cgtacgcgcg cgctagatat ctctccaaca
    16141 cgccgaggct actgaggttg ggtacccgga agggcggaca gccggcaacg ggcaaggagc
    16201 caaggaaagc ggcctcccgg ctttcataca gcggcagcag ctcgagaaag ctcacagggt
    16261 cgcctgccgg gtctgagggc agcgccgaca gatctcgcga gtaaacagca cccagacgtt
    16321 gcgtgaggtc ctcgaagcca cccgtgagac agaacggcac gacaaagata cagaggccga
    16381 gtgcgtagtc cggaacaacc cacttggggt ccagaagtgg acgcaggttc gcgacgaggg
    16441 gaatgagcat gttgcgcgct ccggtgtgct gttgttgagg gaatgaggcc cagacgcgga
    16501 caatactcgc gtggacggcg gccgagacgc tcacgcccag cttcttgcaa cccgccacga
    16561 tggcctcgag cgtcggctcg tcgaacaaag tcaccaggtg ttgggtgttg gcaggcgcgg
    16621 cgtcctcgga cccctcacgc acggggagag acaagctgta caaaccatga tgccaatgcc
    16681 gcatcagctc atcgacggca cgctccacgc ggtgtgggac gggtgtctcc tgcggcggga
    16741 accccagaat atagtcgatg ccgggcggga gggacggttg tttgacatcg gtagtgtagc
    16801 aatctaggtt ggcgtcgagg ccgagacgga agactgactc gagaccgagc atgaaggtgt
    16861 ttgtcgcctt gtatattccc acagcgtcaa atcggaggtg agtgctgcga ataatcaagg
    16921 tcgacgaagg aggaaaccag taggccattg ccgttggacg aggttgaaag atctttccgg
    16981 catcgtccac gtccctgaaa acagcctggg tcccttggtt tacgtggaaa cttagccgca
    17041 gccactcctc aggatccatg aggggaaccg taaccaggta ccgccctgtc tcatctcgtt
    17101 ctgaataggg cggatatatt gcgcctatga gcggctggac atatcgaaaa acaagccaag
    17161 cgcggcggag gtaggggatg gggtcatcaa tgtcttgggg cagtcgtaat ttgagcttgt
    17221 aggtgagacc ctcggcttca cgaccaggag gcgtgttggc gcgttggata cgggcaagca
    17281 gccagttctc caccaagtcg aggggccgtg tgagagagcc gtcttctgat ctggtccatc
    17341 cgggagcgac aatggcatga ttccctgggc tggccatggc tgctctgcct gttaagaaca
    17401 gcggggatag tctgggagag taaagcagtg agggctattg gatattgacg acgaaaacct
    17461 tgcaatgtaa gaagtaagtt tcaaaccagc accataggca agcctcctat ttgggcgact
    17521 ttaccgaatt tactactata tcttagggac atctaaatgc gctaccgcgt tttttgaatg
    17581 cgctaccgcg ttagtcctaa aacagaagaa gtaccttttt acctaattac ttactaaata
    17641 tactagccct ttttagtaat aaatgctagt agcttactgc aatatgcaat gcttatctcc
    17701 ttaagcgtgc taaagcttgt gggtctataa gccctgcaaa ttgtatatat ctcttgttta
    17761 cttttatcta attagatata tatagaccct agttaacgtg actccacaag ctttagcacg
    17821 ctttaggaga taacctttcc ttttcttcct ttttattatt ataaagtctt ataacgtaac
    17881 gtagcgtaat gtaatgtaac gtaataaggc tttaaatatc gcataaatag gcatcttaat
    17941 taacagcgaa aatttacaga tgtaagctac actaaaatta ggtgtgggga ctgtctttcc
    18001 gttttaggac taacgtggta tgcgcattta aaaaacgcgg tatacgcatt tagaggtcta
    18061 atttcgaacg tttttcatta tctccttgga ctctgtccac cttgtgagtt agtggggccc
    18121 ccacaggccc cctgctataa ggttagtaac cacgtaagca cacgaacgta tagtgtagta
    18181 atttatatag ggaaaattag tagtatattt actattagct tatttatata taagcaaata
    18241 tacctttaat ccacttctac ctaattggat atagacccta gtaaacgtga ctccacaagg
    18301 tagacagagt ccaaggagat accaatctac ccctaccttg acaactataa atcccttact
    18361 tacttataag atattaaaag tattaatttt gttatttatg gttatataaa atagctatat
    18421 cttaaagaat aatataataa agggcattaa aaaagagtct tattgcataa tgttacattg
    18481 cattgcgtta tatttgtgat aatctaggct ttggctagac ttagtaatat agttgttgat
    18541 tgcgtaattt aaagggactt gtttcacgcg tatccttttg ctatggtgca attggattag
    18601 gcacatcacc cgtacacgtg ctcgcagaca tccgcgatag cccctcaggc tacaccgtaa
    18661 taaaattgca ttatattgcg ttacattgcg ttgtgttgcg ttgcgttata ttataaggct
    18721 ttttataata ataaaaagga aggaactctt ctattgaaaa attgataggt attatagtaa
    18781 gccacttata tttactactc ataagagcta gtatattcag taagggttta ggtaaaaata
    18841 tgtacttctt ttctcctagg actaacgcgg tatgcgcatt taataaccct aagccaatta
    18901 taaacacatg tccgacttcg cattcgtcat tttcggcggg gaatcatcag agcacacttg
    18961 cctcatcgtg gtgggtttga tggccaaaag gaattcggat atcagccttg cccgcagcgt
    19021 ccgtgccgcg cggggagtct tgctctctct ttgggtctgt ccaatgtccg gcgacctgat
    19081 catatgatca taatcacaca cggagcaccc ataacctggg gtcaatatat aagtcagccc
    19141 atgtgttctc aaaggggctt tctagtgtcc ccatcgccgg ggacttgatc tcgtagcaca
    19201 cgcaccatcc cacccattgc cgccgacggt tcatacacaa cttgatggat ctgacccagt
    19261 tcaacacagc gggcatcgtt tggccgacgg ttgctgccat cgccatctcc tatatcctgc
    19321 tgtcgagctt tctctcttgg tataggctac ggcacatccc cggccctttc ttggcctcga
    19381 tctcaagtct ttggaatgtt ctaaacatcg tgactgggcg cacgtcgcca gtgctcgaga
    19441 aactgccagg aaagtacggc ccgctggtgc gaaccggccc caactacgtt ctcacagatg
    19501 atgccgaaat tttacgtcac gtcaatggcg ctcgcagcac atacccccgt aatgggtgta
    19561 agtctgtcca tatcacatgt cttttgaaat gtagggagac tcagagaccc actcacactg
    19621 tggcttccag ggtatgaagg cttcaaggtc gatgaacacg accatatggg gtcccatatc
    19681 gacacgtcgg tacatgacgc catcaaaagc aaggtgattg gggggtacaa cggcaaggat
    19741 gggatagacc tcgagggggc catcggatcg caggtcaaga ccctggtcag tgagatccgg
    19801 cgccgtcacc ttgggcaacc tgtcgacttc tctcgtctga tgcgtcagat ggcgctcgac
    19861 gccatcaccg ccgtagcctt tggcgaggcc cttgggttcc tgacggccga agacggagac
    19921 gtgttcggct acgtcagcgc cgttgacaag atgctgacct acctgacact tgccagcgac
    19981 ctgcccgtag tgcgcagcgt tgtccggtca cgccgcatgg cgccggcggt gcgttgcgtc
    20041 ctggcctata ctggcattgg ccgcatgctc aaccatacac gccgggtggt ggcggagcgc
    20101 tacgcggccg acgaccccgg gaagggcgac atgacggcct cattcatccg caaggggctc
    20161 acgcagatcg agtgcgaggg cgagagccac ctgcagctca tcgccggcgc cgacactgcc
    20221 gtcacggtgc tgcgctccac gctgctgtac atcatgacga cgccgcgcgt gtacacgcgg
    20281 ctcaaggccg agatcaaggc cgcggtggat gccggcgagg tggtcgaggt catcaccatg
    20341 gcccaggccc agaggctgcc gtatctgcag gctgtcgtgc tcgagggctt ccgcatgcgc
    20401 ccggccgtcg tgtacgggca cttcaagtcg gtgccggccg gcggcgatac gctgccgaat
    20461 ggtgtacgcc tgcctgcagg caccgccatc gcccccaact acatagcact gacccggcgc
    20521 gccgacgtct atggcgccga tgtcgatttg tttcggcccg agcgtttcct cgacgccgag
    20581 ccggccaagc gccacgagat ggagcgcgcc atggacctga acttcgggct tggccgctgg
    20641 cagtgcgctg gcaggaacat tgctctcatg gagatgaaca aggttttctt cgaggtcggt
    20701 ggatgtgcat ctccgctctt tgcttttgtt tcttttcctg ctattactct cgccctcctt
    20761 tgctatcctg acgcgggcga ggtatgagac gagatgagag actgattcaa tgcgcagtta
    20821 ttacgccact tcgacctcca gatcttgtat ccgggcaaag catgggatga atacacgtaa
    20881 ggccttctga aacctttttt aatacctttc gcgcataggc gtctcgggtg gcgtgagcag
    20941 cgtgccatgg atattggagt gctaaccagg ttacctctta cctctgcagg ggcgtggtat
    21001 attcgcagca taacatgtgg gtacaaatca ccgagagctc gtgagagagc gcaaaggtga
    21061 gtatagtgta cagggataca catggcaggg gtggctacga acgtccatga ccacgtacga
    21121 ggcggtaccg atgggcggga aaggacacga ctgagacggc tggagagaac gatgaagatg
    21181 gggtaaggaa ttatagcaat cagaataacg atcgtgtttg tgcgcgtcgg gctttgcctc
    21241 gcactgccta cctaaacaag tcccgaagtg atgaataaaa ttcggcctcg gtgccttccc
    21301 atttgctctg agccaccaat gaagaatcaa cttggttaga aaccctcctc gccaagacca
    21361 tattactagc tgtgataagc accacctgca aatgctgtag gcagcttgtt atttaaaagg
    21421 ccgacaaacc gcagcatctt tggttagctg ggatttagtg gtgccaccta tatgagtccg
    21481 caatctattg tcccatctcc ctgttgagta tctcttcgca tattaccttg agagaattgc
    21541 cggccatctc aaaatcactg acctccaagt atacttgcgc acagagtcta gcccatatcc
    21601 atccgcggta atcgatgatg ggtatccagg tcttgtatct ttccgccaga gcctttgtca
    21661 agtaacgaac tgccgggccg acctctttct ccgtcagagc gccgcccggc acatgagctg
    21721 gggcagtgga cgggcccgtg gcaatggcca agggaagtcg cactgtcacg atgccgcagt
    21781 ttctcatctg gccatctacc ccggccccaa gaccaggctc ctccaagacc tctgtctgaa
    21841 gaatatcggc gatcttgtca ccaccctctt tcgcgagcca ctttatgtac tcgtaaattg
    21901 ccgcctcgcc accacagacg tctcgcctga agcggagggc ggccgggatg cacagatggg
    21961 gcatgtaatc gctcgtggca gtgtaggcga acagtgtctc aaacgaagac gccgtttcgc
    22021 tggcactgac catctgcgac cacaggggaa gtcgagagtt gccattcttg ggaataaatc
    22081 cgaaagaggt cgggatgacg gagcgcatca tgtgctggtt gcgctcggca acgtatagga
    22141 cagcacaagg tcgaggaaca aataaccacc tagtaagatc aatgcgtaga gtatcagcat
    22201 ggaaccgccg tattaagggc gtgtgtgacg ttggatctgc atacttgtgg caatcagaga
    22261 cgaagaagtc aggctgcagc tcctggagat tgacctcgaa ctggcccacg ctatgcgccc
    22321 cgtcgaccaa cgtcatgatg ccttccttct ggcatacgcg cagcaagtct tcgaacggca
    22381 tcctaacagc agggatgctc actatcgtct ctagaattgc taggcgcggg cgtagaccgt
    22441 cagccctgat ttgggccatg gcagtctcaa actgggacac aatcttttgg cccgtcgtgg
    22501 ggagctcaaa ctccacccta cgtgtcttga agggccgagt ctcggccaag gaagtgatgc
    22561 catggtcgat ggcaccgtaa gtagttgata gagtcaccac gacgtcccgt tcctcaaatg
    22621 cctggttgta aaggacggtg aagataccag tagtgacgtt ggagacgagg acgcactccg
    22681 agacggcggc atgaactaga tgagcgaggc cgatacgtgc ctcctgcagc accaagcctt
    22741 gggaaaactc ggagaacaag tcgggctggg cctccagcag ggaccagtaa tctctgatct
    22801 gcttgctcac caccttgggc catgacccac aagatgctac gaccacgtct tgttagttca
    22861 gatacagtca acatggaaat tgaaacgggc acatcgtgtg acgggcaact gacaagaatt
    22921 gaggttggtg tattccggat ccatgcagaa agcctccagc ataggcttgc cgaatgggat
    22981 acgcttgctg ttcactgtca tcttgacaag gcgatgtcac cgaacaagta aatgggaatc
    23041 agtgagagta gagatgccag cagcacttgg tgaaaaggga cacgtcaagt taaagcacac
    23101 ggctggcatt cttatgattt acccaatggg actaaaatca ccccatccac atcccgtggt
    23161 tgattagtag ctgagcagac cacatgcatt gtgtgggcgg ggtgataata tgcaaggaca
    23221 cgtctattcg catctcattc ttttatttac atatgtcgtt tgatcattgg atgcgtctgg
    23281 tcaagcgtgc atatgtatgc ttgcctctgg aaaagttcat ctttctgaaa gggcagtcag
    23341 atatttaata aacttcatta ctccaaccca tacccccctt cgtcgaagct ctctaaataa
    23401 cagtccaacc tacgagacaa tcacggtgaa gccctctgtt tcacttaatc catcatgacc
    23461 gctgcttctt cccctcaccc aggcgtctct gcagaggaca tcgaattcta ccaagccaac
    23521 ggatatcttc gcctgcccca agaggctcac ggcctgttcg acgacttggc aaagttgcag
    23581 gcatgggtgg cagaaatctc ccagtggggc ctggaaacgg ggaaatggcg acattactac
    23641 gagacgacga atggcaagca tcttctctgg gggacggaga agctcatgga ataccacgcg
    23701 cccatgcgag acctgattgc tggcgaggca cctctcacac tgctcaagtc gctgacgggc
    23761 aaagacatgg tggtcttcaa ggacgagata gggtggaaac tcccaggcgg gaagggggcg
    23821 gtccctcacc tcgaccggcc cgcgtactcc atgtttgccc ccgagttcat cgagatcatg
    23881 atcgccgtcg atgcccatac ggtcgagaat ggttgtttac agtttgtacc aggctctcac
    23941 aaggaggcag tcccgatttc ggccgacggc cgcattgcat cggcgtggct agagggcaag
    24001 gagttcatcc ccatggtcct cgatcccggc gacgtcttga tcttcaacga gagcatggcc
    24061 catcggttgg atcctaacaa gacggaccaa agacgtgcag ctgtctttgg cacctaccac
    24121 tttgaccggt cccagcccga cctgcgggac aaattctacg cccaccggct catccacagc
    24181 cccccagaaa acggtaaggc ttttccttgg ccagatgatg tttgcatgtt tggaggccaa
    24241 tgctaacatg atgcgtgacc aatctcacgt agcctgggtt gaaacagtgg aagcgcagac
    24301 ttgacaagaa cgatacgtat aacgtagtct agatagcacg cacaggaagt tagttacgag
    24361 gtagggggtc gaaatggaac ttggcattca ccatgtcaaa tattgtcttc gctataggca
    24421 agcacatttg cacagttgtt gcgtcttatt gttcgtggcc ggtgtaaaat cacacgcccg
    24481 tctttcaata cctagagcac gtttgcacaa tcccgtatct cctgtccttg gcaactttac
    24541 attctgttag atatagcagc ctcacatctt aggcatcact cctataatac cttccctctt
    24601 cgtggctagt tatactgcct tagcacttag ggcaaagacc ctcttttagg cccttatata
    24661 tagccttatt aattaatttt taattttttt tggggttatt agttatagcc aatgcatcta
    24721 atataagaaa aacataatta ctagcgatac ctagctagta taacaattaa catataatta
    24781 atattaacta tagtaataaa taaaacccta tataagaata agctttaaga ttataaatat
    24841 agggggctat aaggttagat taaagtttat aataaacttt atagctatat tagcataaga
    24901 tatagttata gtttataact atagttaaac ttaaatttat ttaagtttta cttatagtat
    24961 aaattaatat aattccccct ttctttataa ctatatttat tcttgtgaga acaaaggata
    25021 gctaggtatg agacggtgtg gaaagtgata atgatgtatt cagctatcta ggctattcac
    25081 acatgtggca taacaaggct ataggtataa gggtaaagag ggtctttact ctaagtccta
    25141 aggtactata atctatagta acctataacc ttaaataagg aagttattat aagtaatacc
    25201 taagatataa ggctactata tttaacacta cccctagtaa aatatctagc ctaaagataa
    25261 agctataaat ataacttatt tactaagata cttactttac taaagtattt ttatttaata
    25321 taaagtctta tacctttatt attt
//
