LOCUS       BGC0000820              8547 bp    DNA     linear   BCT 15-MAR-2014
DEFINITION  Streptomyces griseofuscus strain NRRL 5324 physostigmine
            biosynthetic gene cluster, complete sequence.
ACCESSION   BGC0000820
VERSION     BGC0000820
KEYWORDS    .
SOURCE      Streptomyces griseofuscus
  ORGANISM  Streptomyces griseofuscus
            Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
            Streptomyces.
COMMENT     ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2022-11-07 20:43:34
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 8547
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     gene            1..1008
                     /gene="psmA"
     CDS             1..1008
                     /codon_start=1
                     /gene="psmA"
                     /product="methyltransferase"
                     /protein_id="AHL44339.1"
                     /transl_table=11
                     /translation="MAASATRRLYESFPFPSPGADRPLIDVVGNELPLVVEGGLRDGWS
                     VLDAGCGTGHTLIGLARAYPHVHFVGLEPSDASRDIARHLAERHGTTNVEIVDGAIPDT
                     VIERRFEFIYSYGVVHHLPDPQAGLNWLGDHLAEDGLLHVWLYNAIGEAQRMRDRELVR
                     LMAAHADGHGLDVVRALGFSLSLDAYGMPGAWAGTALDQAEQDVFDADAYLNPVVHTLR
                     FGDLPELLDGVTDWVAADRVYGPHGAPYLDLSGTGGFPAQGPELLRPEDLFDDPGLRTR
                     IGDLGNLDRLRAVELVLRPTGFRALAGRGGSLNRCSRRIAGNLLTGPAGRAQVP"
     source          1..8547
                     /culture_collection="NRRL:5324"
                     /db_xref="taxon:146922"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces griseofuscus"
                     /strain="NRRL 5324"
     misc_feature    1..8547
                     /note="physostigmine biosynthetic gene cluster"
     subregion       1..8547
                     /aStool="mibig"
                     /contig_edge="True"
                     /label="Alkaloid"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..8547
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            1097..2026
                     /gene="psmB"
     CDS             1097..2026
                     /codon_start=1
                     /gene="psmB"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1066:alpha/beta hydrolase domain-containing protein
                     (Score: 242.4; E-value: 9.8e-74)"
                     /gene_kind="biosynthetic-additional"
                     /product="hydrolase"
                     /protein_id="AHL44340.1"
                     /transl_table=11
                     /translation="MLEPRIKERFLDGLNTAPPLEGTTPDALRGAVEELLSFCAPPLPV
                     AQVMDGTTTEATGAVPFRVYHPRPGVPLPLVVYFHGGGWMAGSIAFMDPPLRALAVEAG
                     VVVLSAEYRLAPEHPFPAGLHDAQAVTSWAYENAAALGADPERFAVAGDSAGAALAVVV
                     ALEARGSAVSIGHQLLINPAVDPRMNTASYREFATGYQNSASMMNLCWRTYLNRPDGPL
                     DDAPWQAAPVFAPDLSGLPPTTVITVEYDPLRDEGEAYAARMAAAGVPTTLVRCPGLIH
                     CSLHLDGVAPHAASLRQHAVRALTTAFA"
     gene            2060..2845
                     /gene="psmC"
     CDS             2060..2845
                     /codon_start=1
                     /gene="psmC"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1248:methyltransferase (Score: 102.7; E-value:
                     3.8e-31)"
                     /gene_kind="biosynthetic-additional"
                     /product="methyltransferase"
                     /protein_id="AHL44341.1"
                     /transl_table=11
                     /translation="MADDAHAPMYGAIAEIYDRLDDWIVATWHEQPVPERVAFLRKRWE
                     QRDGEVRDVLDLCCGTGSVLHELKGAGYEVTGLDQSPQMLGLARQRLGDGTALIQAQLP
                     DIPVPDAAMDAVCSTGAALSYTPGEAALAQILLAVHRVLRPGGVFVFDVLSRHMITEHA
                     GRHVWAADQGDFAFIWEFTNPSEKYSEAFYTQFLREGGPDSARYVRTSEKHRLYVLDHS
                     PVRRAAERAGFTTAEVLDNYTDTPAHESTLYDTWVLTRA"
     gene            2861..3664
                     /gene="psmD"
     CDS             2861..3664
                     /codon_start=1
                     /gene="psmD"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1248:methyltransferase (Score: 102.8; E-value:
                     3.4e-31)"
                     /gene_kind="biosynthetic-additional"
                     /product="methyltransferase"
                     /protein_id="AHL44342.1"
                     /transl_table=11
                     /translation="MMQGQPHQDAGMPEPYAATADVYDRLVDYAIAEWGECPRPQMADF
                     VEQAWAARGHRVRRVLELCCGTGLMTEQLVRRGYEVTAVDRSETMLALAKQRVGGAADF
                     HQIELPAPLPDGADAVVCTAAAFNYQASARSLGETLRAVATVLPAGATFVFDIETAALL
                     KGHWGNRVWAADEGDLAFIWDFTSEPDTTYCDVHYTQFTRHEAGADAYTGVREVHRLYA
                     FDHDTVRAQARAAGFAQAEVFDNYTERPATDTTRYETWVLTRDER"
     gene            3668..5335
                     /gene="psmE"
     CDS             3668..5335
                     /codon_start=1
                     /gene="psmE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Carbam_trans_N"
                     /gene_kind="biosynthetic-additional"
                     /product="carbamoyltransferase"
                     /protein_id="AHL44343.1"
                     /sec_met_domain="Carbam_trans_N (E-value: 8.1e-51,
                     bitscore: 162.4, seeds: 4, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLICGLKLTHDGGLAVVEDGRLLFSVEMEKLDNNRRFQPLDRLEL
                     AEDVLAREGVTIGDIDRFVVDGWFAPLGQNQQHPALHVTHHGRPVTLPVAPYQDQPGTD
                     VLTRYTFDGLPLGSATYPYSSYHHSTQHLMGAYATSPFAQRGQDALVLVWDGGMTPVLY
                     RVQPEPLHVTRLGELFPLYGSVFADFCAALPPFRSATTPATPDPHLMPRNLDVAGKAMA
                     YAALGTDDPGFDGVFAAHLDGRDLSFTMGDDLARALAEGDSPPWPDATDADLVASFQGY
                     LGRRLHTALSVALRDGGLPRRLCLAGGCALNIKWNNRLRSSGQFEQVWVPPFPNDSGAA
                     LGAAAVEWSRTGGPAVLDWSVYRGPRLRPAEGDPPGWTSTPCDIEGLARFLHDSERPVV
                     VLTGRAELGPRALGHRSIFASATDPGMKEHLNRIKDRENYRPVAPVCLEHRAPELFSPG
                     TPDPHMLFDHDTRVGWEAKVPAVVHLDGTARLQTVNDREAPFVHTLLIAYEKLSGIPLL
                     CNTSANHKGRGFFPDVASAAAWGRVPAIWSEGRLYRRA"
     gene            5332..5880
                     /gene="psmF"
     CDS             5332..5880
                     /codon_start=1
                     /gene="psmF"
                     /product="N-acetyltransferase"
                     /protein_id="AHL44344.1"
                     /transl_table=11
                     /translation="MNTFRTATARDIPDVAATLTEAFATDPPTQWVFPDGTAAVSRFFT
                     HVADRVHTAGGIVELLPDRAAMIALPPHVRLPGEAADGRQAEIQRRLADRHPLTPHYYL
                     LFYGVRTAHQGSGLGGRMLARLTSRADRDRVGTYTEASTWRGARLMLRHGFHATRPLRL
                     PDGPSMFPLWRDPIHDHSD"
     gene            5864..7069
                     /gene="psmG"
     CDS             5864..7069
                     /codon_start=1
                     /gene="psmG"
                     /product="putative tryptophan 2,3-dioxygenase"
                     /protein_id="AHL44345.1"
                     /transl_table=11
                     /translation="MTTPTERAERAVDTWLQRIRKGPPGPFPHRLVTRAVQAHGSTALP
                     ERLRGQLIDAWDWSRSCSLEPGSEEWATAAWLECLTSSLRNAPSYGAYTMTSLFGRWSR
                     LGDFPRRNAQLSALLTADLIRYELRRATHEDHLGERRSRSTAATLLARHLDEDRGWAET
                     TCRPKPEGGSQAIQRCNHLLSAGGRGLPAPLALLVESSLPVATTEPDEPLFIRSVQIME
                     LLAEQAAFHLGEAHRRARAATPEPSSLAMLLHATADSLDRAQHVFRLVSTIDPTQFAAI
                     RAATAGTGALQSTGFARLERCCRGEAGLRPETVAAVGLVTAPTPAPSLSTTLARVTTRL
                     ETHDPTAAGHLRLAIQAVNAQWQRWKRTHLGVAKKIIGDVPGTGGTDGVRYLARHLEEP
                     LL"
     gene            7066..8547
                     /gene="psmH"
     CDS             7066..8547
                     /codon_start=1
                     /gene="psmH"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1180:Decarboxylase, pyridoxal-dependent (Score: 361.7;
                     E-value: 8.4e-110)"
                     /gene_kind="biosynthetic-additional"
                     /product="PLP-dependent decarboxylase"
                     /protein_id="AHL44346.1"
                     /transl_table=11
                     /translation="MKPAEAKPPHMDHDTFRSLGHQAIDWIADYWQHLADRPVAPLVEP
                     GTVRAQLPTTPPEYGEDFPALLSDLERIVLPGLLHWQHPRFFGYFPANASGPAVLAELL
                     SAGLGVQGMNWNTSPACTEVEQQMLDWFVHLLGLPEHFRGGGVIQDTASSAVLVALLTA
                     LHRASAGRTRDHGTGECGYRVYLTAETHSAARKAAVITGLGLRAVCEVATDADGAMDAA
                     DLAARIQADLAAGLTPLMVVATRGTTSDLSFDPLEDIGPVCRRHGVWLHVDAAYAGVAA
                     VCDELHWVNDGVRYADSYCTNPHKWLLTNFDCDLLWVADPEALVNALSVLPAYLRNPAS
                     ESGRVTDYRHWQVPLGRRFRALKLWSVLRWYGAEGLRSHIRTGVRHARLFADLVREDDR
                     FTLLAPATLGLVTFRHAGSDTDNRNLLHAINAEGTTFLTHSEKNGTFFLRFATGGTHTE
                     DHHVHEAWRAVQNAVQNAVPRAQHATDGTADALNG"
ORIGIN
        1 atggccgcaa gtgcaacgcg tcgcctttat gagagtttcc cttttccttc gccgggagcc
       61 gaccggccgc tgattgacgt cgtcggaaac gagctgcccc tggtggtcga aggcgggctg
      121 cgtgacggat ggtcagtgct cgacgcgggg tgcggcaccg ggcacaccct gatcggtctg
      181 gcccgcgcgt atccccacgt gcactttgtg gggctcgaac cgtccgacgc ctcacgcgac
      241 atcgcacggc atctggccga gcggcacggc accacgaatg tggaaatcgt cgacggcgca
      301 attccggaca ccgtaatcga gcgccgtttt gaattcatct acagctacgg tgtcgtccac
      361 cacctgccag atccgcaggc agggctcaat tggcttggcg accacctggc cgaggacgga
      421 ttactgcatg tgtggctgta caacgccatc ggtgaggctc agcgcatgcg tgaccgcgaa
      481 ctggtccggt tgatggccgc gcatgcggac ggccatggac tcgacgtcgt cagggctctc
      541 gggttctccc tgtcgctcga cgcctacggt atgcccgggg cctgggccgg caccgcgctg
      601 gaccaggcgg aacaggacgt cttcgacgcg gacgcctacc tcaatcccgt ggtgcacacc
      661 ctgcggttcg gtgacctccc ggagctgctg gacggagtga ccgactgggt ggccgccgat
      721 cgcgtctacg ggccgcacgg cgcgccctac ctcgacctga gcggcaccgg cgggtttccc
      781 gcgcaggggc ccgagctgct gcgccccgaa gacctcttcg acgaccccgg gctgcggacc
      841 cggatcggcg acctcggcaa tctcgatcgt ctacgggcgg tggaactcgt cctgcggccc
      901 accggcttcc gcgcactggc cggacggggc ggttccctga accggtgttc ccggcgcatc
      961 gccggcaatc tcctgaccgg acccgccggc cgggcccagg tcccctgagc gccaggccgg
     1021 cacgccgtac gaggccggct ccagggcgaa gaagcggaca tctcctgcgg ctcccaagga
     1081 aaggtccttc acctccgtgc tcgaaccccg catcaaagaa cgcttcctcg acggtctcaa
     1141 cacggccccg ccgttggaag ggacgactcc cgacgccttg cgcggagcgg ttgaggaact
     1201 gctctccttc tgcgcgccgc ctctccccgt cgcccaggtc atggacggca cgaccacgga
     1261 agccacgggg gcagtgccgt tccgggtcta ccatccgcgg ccgggggtcc ccctcccgct
     1321 ggtcgtgtac ttccacggcg gcggctggat ggccggcagc atcgcgttca tggatcctcc
     1381 gctgcgcgcc ctggcggtcg aggccggcgt ggtggtgctg agcgcggagt accggctcgc
     1441 gccggagcat cccttcccgg ccgggctgca cgacgcgcag gccgtcacct cctgggccta
     1501 cgagaacgcc gccgcgctcg gggccgaccc cgagcggttc gcggtcgccg gggacagtgc
     1561 gggcgcggct ctcgccgtcg tcgtcgcact tgaggcccgt ggctcggccg tctccatcgg
     1621 ccaccagctc ctgatcaatc cggccgtcga cccgcggatg aacaccgcct cctaccggga
     1681 gttcgcgacg ggctaccaga acagcgcgtc gatgatgaac ctgtgctggc gcacgtacct
     1741 gaaccggccc gacgggcccc tggacgacgc gccctggcag gccgccccgg tcttcgcccc
     1801 ggacctctcc ggcctgccgc cgaccaccgt catcacggtc gagtacgacc cgctgcgcga
     1861 cgagggcgag gcatacgccg cgcggatggc cgccgccggg gtgccgacca cgctggtgcg
     1921 ctgcccgggt ctcatccact gctcactcca cctggacggc gtagcccctc acgccgcctc
     1981 cctacggcag cacgcggtgc gggcgctgac caccgcgttc gcctgatcca gaccctgttc
     2041 cagacagaga ggacctggca tggcagacga cgcgcacgcc ccgatgtacg gggccatcgc
     2101 tgagatctac gaccgactgg atgactggat cgtcgccacc tggcacgagc agccggtccc
     2161 cgaacgggtg gccttcctgc gcaagcggtg ggagcagcgc gacggcgagg tgcgggacgt
     2221 gctggacctg tgctgcggca ccggcagcgt gctgcatgag ctcaaagggg ccggctatga
     2281 ggtcaccggg ctcgaccagt ccccgcagat gctcggcctc gcccggcagc gcctcggtga
     2341 cggaaccgca ctgatccagg cgcaactgcc cgacatcccg gtgcccgacg ccgccatgga
     2401 cgcggtgtgt tccaccggcg cggcgctgag ctacaccccc ggtgaggcag cgctggccca
     2461 gatcctcctg gccgtgcacc gcgtcctgcg ccccggcggc gtcttcgtgt tcgacgtcct
     2521 gtcccgccac atgatcaccg aacacgcggg ccggcacgta tgggccgccg atcagggcga
     2581 cttcgccttc atctgggagt tcaccaaccc cagcgagaag tacagcgaag ccttctacac
     2641 gcagttcctc cgggagggcg gaccggattc cgccaggtac gtacgcacca gcgagaagca
     2701 tcgcctgtac gtcctcgacc actccccggt gcggcgcgcc gccgagcggg ccggcttcac
     2761 caccgcagag gtgctcgaca actacaccga cacccccgcc cacgagagca cgctctacga
     2821 cacgtgggtc ctgacccgcg cctgaggagg gaaaccgagc atgatgcagg gacagccgca
     2881 ccaggatgcg ggcatgcccg agccgtacgc cgcgaccgcc gacgtgtacg accggctcgt
     2941 cgactacgcc atcgccgagt ggggagagtg cccccggccg cagatggccg acttcgtcga
     3001 gcaggcatgg gcggctcgcg ggcaccgcgt gcgccgggtg ctcgaactgt gctgcggcac
     3061 cgggctgatg accgagcaac tggtccggcg cggttacgag gtcaccgccg tcgaccgttc
     3121 cgagaccatg ctggccctgg cgaagcagcg ggtgggcggt gcggccgact tccatcagat
     3181 cgaactgccc gccccgctgc ccgacggtgc ggatgccgtg gtgtgcaccg cggccgcctt
     3241 caactaccag gccagcgccc gatctctggg agagaccctg cgcgccgtgg ccacggtgct
     3301 gccggcgggt gcgacgttcg tcttcgacat cgagaccgcc gcactcctca agggacactg
     3361 gggcaaccgc gtgtgggccg ccgacgaggg cgatctggcg ttcatctggg acttcacgag
     3421 tgagccggac accacctact gcgatgtgca ctacacccag ttcacccgcc acgaggcggg
     3481 agcggacgcc tacaccggcg tccgcgaggt gcaccggctg tacgccttcg accacgacac
     3541 cgtccgcgcc caggcgcgtg ccgccggatt cgcgcaggcg gaggtgttcg acaactacac
     3601 cgagcgcccc gccaccgaca ccacccgcta tgagacctgg gtactcaccc gagacgagcg
     3661 ctgagccatg ctgatctgcg gactgaaact cacccacgac ggtggcctgg ccgtggtgga
     3721 agacggcagg ctgttgttct ccgtggaaat ggagaagctg gacaacaacc ggcgattcca
     3781 accgctggac cggctggagc tcgcggagga cgtcctcgcc cgggaaggcg tcacgatcgg
     3841 cgacatcgac cggttcgtgg tggacggctg gttcgccccg ctgggccaga accagcagca
     3901 cccggcgctg cacgtgaccc accacgggcg gccggtcacg ctgcccgtcg ccccctacca
     3961 ggaccagccc ggcacggacg tcctgacccg atacaccttc gacggccttc cgctcggctc
     4021 cgccacgtac ccctacagca gctaccacca cagcacccag cacctgatgg gcgcctacgc
     4081 caccagcccc ttcgcccagc gcggacagga cgcgctggta ctggtgtggg acggcggcat
     4141 gacgcccgtc ctgtaccggg tgcagcccga acccctccac gtgacacgcc tgggcgagct
     4201 gttccccctg tacggcagcg tcttcgcgga cttctgcgcc gcccttccgc cgtttcgcag
     4261 cgccaccacg ccggccacgc ccgacccgca cctcatgccc cgcaacctgg acgtggcagg
     4321 caaggccatg gcgtacgccg cgctcggcac ggacgacccg ggcttcgacg gcgtcttcgc
     4381 ggcgcacctc gacggaaggg acctgtcctt caccatgggc gacgacctcg cccgcgccct
     4441 cgccgaaggc gacagtcccc cgtggccgga cgccacggac gccgacctcg tcgccagctt
     4501 ccagggctat ctgggccgcc gactgcacac cgccctcagc gtcgccctgc gcgacggcgg
     4561 cctgccgcgc cgcctgtgcc tggccggcgg ctgcgcactg aacatcaaat ggaacaaccg
     4621 gctgcgctcc tccggacagt tcgagcaggt atgggtgccg ccgttcccca acgactccgg
     4681 ggccgccctg ggggccgccg ccgtggagtg gtcacggacc ggcgggccgg ccgtcctgga
     4741 ctggtccgtg taccggggcc cccggctccg gcccgcggag ggcgatccgc cgggctggac
     4801 ctccaccccc tgcgacatcg agggactggc gcgcttcctc cacgacagtg aacggcccgt
     4861 cgtcgtcctc accggacgag cggaactggg gccacgagcc ctggggcacc gcagcatctt
     4921 cgcgtcggcc accgaccccg gcatgaaaga gcacctcaac cgcatcaagg accgcgagaa
     4981 ctaccggccc gtcgccccgg tgtgcctgga acaccgggcc ccggagctgt tctccccggg
     5041 cacccccgac ccccacatgc tcttcgacca cgacacccgc gtcggctggg aagccaaggt
     5101 ccccgccgtc gtacacctgg acggcaccgc acgtctgcag accgtcaacg accgggaggc
     5161 gcccttcgtc cacacgctgc tgatcgccta cgagaagctg tcgggcatcc cgctgctctg
     5221 caacaccagc gccaaccaca aaggccgcgg tttcttcccg gacgtcgcgt cggccgcggc
     5281 ctggggacgc gtaccggcca tctggtccga aggccgcctc taccgccgcg catgaacacc
     5341 ttccggaccg cgacggcacg cgacatcccc gatgtcgccg ccaccctcac cgaagccttc
     5401 gccactgacc caccgaccca atgggtcttc ccggacggca ccgcggcggt ctcccgcttc
     5461 ttcacccacg tcgccgaccg cgtccacaca gcaggcggga tcgtcgaact cctgcccgac
     5521 agagccgcga tgatcgccct gcccccgcac gtacgactgc ccggcgaagc agccgacggc
     5581 agacaggccg agatccagcg ccggctggcc gaccgccacc cgctcacccc gcactactac
     5641 ctcctgttct acggcgtgcg cacggcccac cagggctcag ggctgggcgg gcggatgctc
     5701 gcccgactca cctcccgggc cgaccgcgac cgcgtgggca cctacaccga ggccagcacc
     5761 tggcgcggcg cccgcctgat gttgcgtcac ggcttccacg ccacgcggcc gctgcggctt
     5821 cccgacgggc cgtccatgtt ccccctctgg agagacccga tccatgacca ctccgactga
     5881 gcgcgcggag cgtgcagttg acacctggct ccaacgcatc cgcaaaggcc cacccggacc
     5941 gttcccgcac cggctggtca cccgcgccgt gcaggcccac ggcagcacgg cgctgcccga
     6001 gcgcctgaga gggcagctca tcgatgcctg ggactggtcc cgcagctgct ccctcgaacc
     6061 cggcagcgag gagtgggcga ccgctgcctg gctggagtgc ctgaccagtt ccctgcgcaa
     6121 cgctccctcc tacggcgcgt acacgatgac ctcgctgttc ggccggtgga gccgcctggg
     6181 cgacttcccc cggcgcaacg cacaactgtc cgcgctgctc accgccgacc tgatccggta
     6241 cgaactacgg cgtgccaccc acgaggacca cctcggcgaa cgccgctccc gcagcacggc
     6301 cgccaccctc ctcgcccgcc atctggacga ggaccgcggc tgggccgaaa ccacctgccg
     6361 acccaaaccc gaggggggct cgcaggccat acagcgctgc aaccatctgc tgtccgccgg
     6421 cggccgggga ctacccgctc ccctcgcact gttggtggag tcgtcacttc cggtggcgac
     6481 gacggagccg gacgagcccc tgttcatccg ctccgtacag atcatggaac tcctcgccga
     6541 gcaggccgcg ttccacctcg gcgaggcaca ccggcgggcc cgcgccgcca ccccggagcc
     6601 gtcctcgctg gccatgctgc tgcatgccac cgccgacagt ctggaccgcg cccaacacgt
     6661 cttccgcctg gtgtccacca tcgatcccac ccagttcgcc gccatccgcg cggccaccgc
     6721 gggcacgggc gcgttgcagt caacgggctt cgcccgactg gagcgctgct gccgtggtga
     6781 ggccgggctg cgcccggaga ccgttgccgc cgtcgggctg gtgacagccc cgacccccgc
     6841 cccctccctg tccaccacac tcgcgcgggt caccacccgg ctggagacac acgacccgac
     6901 cgcggccgga cacttacgcc tggccatcca ggcagtgaac gcacagtggc aacggtggaa
     6961 gcgcacccac ctcggtgtcg cgaagaagat catcggcgat gtgccgggca ccggcggaac
     7021 ggacggtgtg cgctacctcg cccgtcacct tgaggagcca ctcctgtgaa gcccgctgag
     7081 gcgaaaccgc cccacatgga ccacgacacc ttccgctccc tgggccacca ggcgatcgac
     7141 tggatcgccg actactggca gcatctggcg gaccggcccg tcgccccctt ggtcgaaccc
     7201 ggcaccgtcc gcgcccagct gccgacgacc ccgcccgaat acggcgagga ctttcccgcg
     7261 ctcctctccg acctcgaacg gatcgtgctg ccgggcctgc tgcactggca acacccccgc
     7321 ttcttcggct acttccccgc gaacgcctcc ggccccgccg tcctggccga gctgttgtca
     7381 gcgggcctgg gcgtccaggg gatgaactgg aacaccagcc cggcctgcac cgaggtcgag
     7441 cagcagatgc tggactggtt cgtgcacctg ctcggcctgc ccgagcactt ccgtggcgga
     7501 ggggtcatcc aggacaccgc ctccagcgcc gtcctggtgg cgctgctcac cgccctgcac
     7561 cgggccagcg cgggccgcac ccgcgaccac ggcaccggcg agtgcggcta ccgggtctac
     7621 ctgaccgccg agacgcactc ggcggcccgc aaggccgccg tcatcaccgg actgggcctg
     7681 cgcgccgtgt gcgaggtggc caccgacgcc gacggcgcca tggacgcagc cgacctggca
     7741 gcccgcatcc aggccgacct cgccgccggc ctgaccccgc tgatggtcgt ggccacccgc
     7801 ggtaccacct ctgatctctc cttcgatccc ctggaggaca tcggccccgt ctgccgccgg
     7861 cacggcgtgt ggctccacgt cgacgccgcg tacgccggtg tggccgcggt ctgcgacgaa
     7921 ctgcactggg tcaacgacgg cgtgcgctac gcggactcgt actgcaccaa cccgcacaag
     7981 tggctgctga ccaacttcga ctgcgacctg ctgtgggtgg ccgaccccga agccctcgtc
     8041 aacgccctga gcgtgctccc cgcatacctg cgcaacccgg cctccgaatc gggccgggtc
     8101 accgactacc gccactggca ggtcccactg ggccgccgct tccgcgcact gaaactgtgg
     8161 tccgtgctcc gctggtacgg cgccgagggc ctccgctccc acatccgcac cggcgtacgc
     8221 cacgcccgac tcttcgcaga cctggtccgc gaagacgacc gcttcaccct gctcgccccg
     8281 gccaccctgg gcctggtgac gttccgtcac gcaggatccg acacggacaa ccgaaacctc
     8341 ctgcatgcca tcaacgccga gggaaccacc ttcctcaccc actccgagaa gaacgggacc
     8401 ttcttcctcc gcttcgccac cgggggcacg cacacggagg accaccatgt tcacgaagca
     8461 tggcgcgccg tccagaacgc cgtccagaac gccgtcccac gcgctcaaca cgccaccgac
     8521 ggcacggctg acgcgctgaa cgggtag
//
