LOCUS       BGC0000837             18539 bp    DNA     linear   BCT 22-MAR-2017
DEFINITION  Vibrio fischeri ES114 chromosome I, complete sequence.
ACCESSION   BGC0000837
VERSION     BGC0000837
KEYWORDS    .
SOURCE      Aliivibrio fischeri ES114
  ORGANISM  Aliivibrio fischeri ES114
            Bacteria; Pseudomonadota; Gammaproteobacteria; Vibrionales;
            Vibrionaceae; Aliivibrio.
COMMENT     On Apr 2, 2008 this sequence version replaced CP000020.1.
            ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 14:17:59
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             complement(1..1233)
                     /EC_number="1.-.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Trp_halogenase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1119:halogenase (Score: 242.5; E-value: 1.6e-73)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="VF_0841"
                     /old_locus_tag="VF0841"
                     /product="dehydrogenase"
                     /protein_id="AAW85336.1"
                     /sec_met_domain="Trp_halogenase (E-value: 2.4e-19,
                     bitscore: 59.8, seeds: 23, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLSPQTTQVAIIGAGPSGSIAAALLNKHNINCIILEKSTFPRFSI
                     GESLLPACMESIKHAGMLNAVEDACFQFKDGAAFHCNGTFTSFDFTDKFTDGAGTTFQV
                     QRGNFDKVLADCAKEQGVEIRYQHEITDVDVNAEKPLLSVLDENGTPYQIQADFILDAS
                     GFGRVLPKLLELEEPSCLPPRRAIFTHIKDNITDESYDRNKILVSVHPTNTQVWYWLIP
                     FSNGTCSFGVVGEPEFFDAYSEDKVTALKQLAEEESNLKHLLNQAEYPNPCGEIMGYSA
                     NVSKLFDKNFALLGNAGEFLDPVFSSGVTIAMKSAELAADLLIKQFNQEDVNWQQDYSD
                     KLMKGVNAFRCYVEGWYEGTFQDVIFHENPNPKIKQMICAILAGYAWDESNPFVAEPER
                     RLKVVAELCKP"
     protocluster    1..18539
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[14913:18539]"
                     /cutoff="20000"
                     /detection_rule="(APE_KS1 or APE_KS2)"
                     /neighbourhood="20000"
                     /product="arylpolyene"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      14914..18539
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(APE_KS1 or APE_KS2)"
                     /neighbourhood="20000"
                     /product="arylpolyene"
                     /protocluster_number="1"
     cand_cluster    1..18539
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(APE_KS1 or APE_KS2)"
                     /kind="single"
                     /product="arylpolyene"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..18539
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000837"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..18539
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="arylpolyene"
                     /region_number="1"
                     /rules="(APE_KS1 or APE_KS2)"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            complement(1..1233)
                     /locus_tag="VF_0841"
                     /old_locus_tag="VF0841"
     gene            complement(1333..2400)
                     /locus_tag="VF_0842"
                     /old_locus_tag="VF0842"
     CDS             complement(1333..2400)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1042:O-methyltransferase (Score: 135.4; E-value:
                     4.5e-41)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="VF_0842"
                     /old_locus_tag="VF0842"
                     /product="putative O-methyltransferase"
                     /protein_id="AAW85337.1"
                     /transl_table=11
                     /translation="MTQYKNDPFNALEAKTEAQKLSFAPIVFHTARTLRDLGILAALDK
                     AQDQGLDAEQLAQETGVSEYGVKVLLDMALSAHIVTWNKPNYVLANLGHFLLHDGMTNA
                     NLDFTADVCYAAMMHLTEAIQEGTPAGLKELGDWPTIYEGLSRLPEKAKESWFKFDHFY
                     SDRSFPILLEKVLSQKPKRLFDIGGNTGKWALQCCNYDNDVEVTIIDLPQQIEMALKNA
                     KEQGFADRINGHPTNMLDKTLPLPQGADVWWMSQFLDCFSPMEILSILKRVRAAMSTDA
                     KVYILELFWDAQKYDAASFSLNATSLYFTCLANGNSRFYRCSDFLEIIEEAGFAVAEQT
                     DNIGLGHTLLELRAK"
     gene            complement(2504..3079)
                     /locus_tag="VF_0843"
                     /old_locus_tag="VF0843"
     CDS             complement(2504..3079)
                     /codon_start=1
                     /locus_tag="VF_0843"
                     /old_locus_tag="VF0843"
                     /product="lipoprotein, putative"
                     /protein_id="AAW85338.1"
                     /transl_table=11
                     /translation="MSFLKPLLATSGLIILSGCASVMTLTDKNTGDDLFLTNKPVYTLV
                     NLHPDDTRSKLYSVNFQQDGLIPLCTQVDIVNVSTKRLTFKIDSSGREYILDKHKSSPD
                     FAKYLSNYFGTECNTEKVEKLSALDQQGIKEGVVKKGMTKEGVKYAIGFPPEHRTPNLN
                     SDHWLYWKNRFNTFKVEFNGNTVSNIID"
     gene            3209..3937
                     /locus_tag="VF_0844"
                     /old_locus_tag="VF0844"
     CDS             3209..3937
                     /codon_start=1
                     /locus_tag="VF_0844"
                     /old_locus_tag="VF0844"
                     /product="hypothetical protein"
                     /protein_id="AAW85339.1"
                     /transl_table=11
                     /translation="MDIMTYANFNIIDWHAISAGLDSKNAWLEWRENNFTWPENEKAPV
                     DKIPAMARRRMSSLSKLAVQTALTLAEENDIDYLVFSSQHGELTRTVALLEDILNGDDA
                     SPMAFSQSVHNTAAGLFTITAKKAIPVTSIAAGENTFHSAVVEAAAYLAENQNNKVLII
                     DFDEPLPSHYQEFEKQNYQGYAVGLIMSAGDIFSLQWNNKPTIKPSGLPHGLQCIANLV
                     GSHSQWITESSSKQWVWNRK"
     gene            3942..4739
                     /locus_tag="VF_0845"
                     /old_locus_tag="VF0845"
     CDS             3942..4739
                     /EC_number="2.3.1.51"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1141:acyltransferase (Score: 70.9; E-value: 1.6e-21)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="VF_0845"
                     /old_locus_tag="VF0845"
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="AAW85340.1"
                     /transl_table=11
                     /translation="MTLLQRTDKYWRILATGFCFSLFGVGALCLTFIVFPLLTFTVKDK
                     KQREVRVQSIIQRSFFIFCKTMHYSRAIKYQFDGAELLQSDRNCLIVANHPSLIDYVLI
                     ASQLKQCDCIVKAALWHNPFTQGIVRAAGYIPNQEPEAFLEECSEKLNSGNVLLVFPEG
                     TRTTPGKKSKLQRGAAQIAVRSQSDIRIIHITVTPSFLTKEAKWYQVPDVRPFFHLQVK
                     ERVAITPFIEDADSSSSAARNVNRFLDTALYPQNEVNTSKENK"
     gene            4708..5004
                     /locus_tag="VF_0846"
                     /old_locus_tag="VF0846"
     CDS             4708..5004
                     /NRPS_PKS="Domain: PP-binding (19-93). E-value: 9e-12.
                     Score: 37.3. Matches aSDomain:
                     nrpspksdomains_VF_0846_PP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="VF_0846"
                     /old_locus_tag="VF0846"
                     /product="acyl carrier protein"
                     /protein_id="AAW85341.1"
                     /sec_met_domain="PP-binding (E-value: 3.2e-12, bitscore:
                     37.3, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKSTHQKKISRPLMEVLHNEIKQLIIDALNLEDISVDDIETEAPL
                     FGDGLGLDSIDALELGLAIKKKYNLVLDADDTQTREHFASVANLAAYINANKA"
     aSDomain        4765..4986
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_VF_0846_PP-binding.1"
                     /evalue="9.00E-12"
                     /label="VF_0846_PP-binding.1"
                     /locus_tag="VF_0846"
                     /protein_end="93"
                     /protein_start="19"
                     /score="37.3"
                     /tool="antismash"
                     /translation="EIKQLIIDALNLEDISVDDIETEAPLFGDGLGLDSIDALELGLAI
                     KKKYNLVLDADDTQTREHFASVANLAAYI"
     gene            5016..5273
                     /locus_tag="VF_0847"
                     /old_locus_tag="VF0847"
     CDS             5016..5273
                     /NRPS_PKS="Domain: PP-binding (11-76). E-value: 1.7e-14.
                     Score: 46.0. Matches aSDomain:
                     nrpspksdomains_VF_0847_PP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="VF_0847"
                     /old_locus_tag="VF0847"
                     /product="acyl carrier protein"
                     /protein_id="AAW85342.1"
                     /sec_met_domain="PP-binding (E-value: 6.2e-15, bitscore:
                     46.0, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTDVNKEQVLEQVTSALVELFEIDEADITPEAQLYTDLDLDSIDA
                     VDLVVHLQKVTGKKIKPEEFKEVRTVDDVVNSVAALLKGE"
     aSDomain        5049..5243
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_VF_0847_PP-binding.1"
                     /evalue="1.70E-14"
                     /label="VF_0847_PP-binding.1"
                     /locus_tag="VF_0847"
                     /protein_end="76"
                     /protein_start="11"
                     /score="46.0"
                     /tool="antismash"
                     /translation="QVTSALVELFEIDEADITPEAQLYTDLDLDSIDAVDLVVHLQKVT
                     GKKIKPEEFKEVRTVDDVVN"
     gene            5273..5833
                     /locus_tag="VF_0848"
                     /old_locus_tag="VF0848"
     CDS             5273..5833
                     /EC_number="5.99.1.3"
                     /codon_start=1
                     /locus_tag="VF_0848"
                     /old_locus_tag="VF0848"
                     /product="DNA gyrase subunit B"
                     /protein_id="AAW85343.1"
                     /transl_table=11
                     /translation="MRWLTALSAIALLAYPLAVYYGLHFGGIKTVAGVLAVLFLVRMVG
                     GKQVPIKELKYIAIISGGAGIILASLGWIFKTEGWFTFYPVIVNVVMFVMFFWSLFQSQ
                     SMIERFARLQEPDLPESGVKYTRTVTKIWCVFFIINGSISFSTCFMDMKMWMLYNGFIS
                     YLLIGTLFISEWLVRQWIRKKEV"
     gene            5794..7197
                     /locus_tag="VF_0849"
                     /old_locus_tag="VF0849"
     CDS             5794..7197
                     /NRPS_PKS="Domain: AMP-binding (25-297). E-value: 1.2e-17.
                     Score: 55.4. Matches aSDomain:
                     nrpspksdomains_VF_0849_AMP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002:AMP-dependent synthetase and ligase (Score: 49.7;
                     E-value: 3.3e-15)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="VF_0849"
                     /old_locus_tag="VF0849"
                     /product="AMP-(fatty)acid ligase"
                     /protein_id="AAW85344.1"
                     /sec_met_domain="AMP-binding (E-value: 4.3e-11, bitscore:
                     32.3, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVSAPMDKKKRSMKTVSLSQLVLQDKTDHTVCFDEQHSYISYSQF
                     QKDVQKACAFFQQQPHTKWALCFDDSYYFSVTFLALAHAGKHLILLGNHQPAALAELSQ
                     HFDALIHDDIIKVMPCSEHVAYHDIKSDHLATLSPLPLHEVELTLFTSGSTDTPKPIAK
                     NLQLLDNEIQQLDCLWGEKLGQSDIYSTVSHQHIYGLLFRVLWPLTAGRAFSRHNFIYP
                     EQILSHRPDDKKTLISSPALLKRLVDHEGYNGYQMVFSSGGPLAIEGAEQSEKKLSLRP
                     FEVYGSTETGGIGYRQQFEPSSLWCFFPSHQARLNIEHCLELLSPFIDDEEWYATSDYC
                     DLLANGQFVLKGRVDRVIKIEEKRLSLTEVEKRLMQLEWINEAAVIPMEEEKRTVLGAV
                     IVLSEAGTKLMEQGSKGKFWLRLRSELREWLEPVGIPRRFRTVSEIPLNSQGKRQMHDL
                     KQLFNDANN"
     aSDomain        5869..6684
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_VF_0849_AMP-binding.1"
                     /evalue="1.20E-17"
                     /label="VF_0849_AMP-binding.1"
                     /locus_tag="VF_0849"
                     /protein_end="297"
                     /protein_start="25"
                     /score="55.4"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="KTDHTVCFDEQHSYISYSQFQKDVQKACAFFQQQPHTKWALCFDD
                     SYYFSVTFLALAHAGKHLILLGNHQPAALAELSQHFDALIHDDIIKVMPCSEHVAYHDI
                     KSDHLATLSPLPLHEVELTLFTSGSTDTPKPIAKNLQLLDNEIQQLDCLWGEKLGQSDI
                     YSTVSHQHIYGLLFRVLWPLTAGRAFSRHNFIYPEQILSHRPDDKKTLISSPALLKRLV
                     DHEGYNGYQMVFSSGGPLAIEGAEQSEKKLSLRPFEVYGSTETGGIGYRQ"
     CDS_motif       6238..6276
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_VF_0849_0001"
                     /evalue="2.40E-03"
                     /label="NRPS-A_a3"
                     /locus_tag="VF_0849"
                     /protein_end="161"
                     /protein_start="148"
                     /score="11.0"
                     /tool="antismash"
                     /translation="LFTSGSTDTPKPI"
     CDS_motif       6847..6906
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_VF_0849_0002"
                     /evalue="2.20E-03"
                     /label="NRPS-A_a8"
                     /locus_tag="VF_0849"
                     /protein_end="371"
                     /protein_start="351"
                     /score="10.9"
                     /tool="antismash"
                     /translation="GRVDRVIKIEEKRLSLTEVE"
     gene            7211..7585
                     /locus_tag="VF_0850"
                     /old_locus_tag="VF0850"
     CDS             7211..7585
                     /codon_start=1
                     /locus_tag="VF_0850"
                     /old_locus_tag="VF0850"
                     /product="AMP-(fatty)acid ligase"
                     /protein_id="AAW85345.1"
                     /transl_table=11
                     /translation="MIKRKPSILTTKDISVNEAQPSVLICMRVDDDILDFTGHFPNHPL
                     LPGVTQVDWAIHYAKHYLNAHSNFKGMEVLKFQEPILPNMEIELELKWDIEKSKLYFTY
                     RSNKGDTVSNHASGRIVLGA"
     gene            7588..9279
                     /locus_tag="VF_0851"
                     /old_locus_tag="VF0851"
     CDS             7588..9279
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1123:polyprenol-monophosphomannose synthase ppm1
                     (Score: 85.5; E-value: 7.6e-26)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="VF_0851"
                     /old_locus_tag="VF0851"
                     /product="predicted acyltransferase"
                     /protein_id="AAW85346.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 1.6e-26,
                     bitscore: 83.6, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTNFRPCFLIPCYNHGKTIPAVVESLMSYGYPMIIVDDGSENETK
                     RILEEVTQHNESITLITLAENQGKGGAVIAGIEKAYQQSYSHAIQIDADGQHDLEALPK
                     LIAESQEHPTALISGQPIYDESVPKSRLYGRYATHIWVWIETLSFTIKDSMCGFRSYPI
                     GPTINVLERAVLGRRMDFDTEIMVRMYWNETDIRFINTRVIYPEDGISHFDALWDNVKI
                     SWMHTKLFFGMLPRIPSLLKRKPVQEDHWSSHAERGTILGIQFLLWIYSTFGRKVFSLL
                     LKPVMAYYYLRGGSVKDASDDFIQQVNAYARIKGIQLPEKLTTYRHLVSFGETMLDKLA
                     AWRGDFSDKNLTVHGIEHYQTLAQREKGIVLLGSHLGNLELCRALSRRHTHLKINALVF
                     TEHAERFNTVMKTVNPQSEVNVIQVSKIGPDTAILLQQKIDDGEWIVIVGDRTSVTKEN
                     RVIWADFLGKPAPFPQGPFMLASILKQPVYLMFGLRDDTQKDPLFDVYLEPFSEQIILP
                     RGKREEALQEVVQNYAQRLEHFTLKAPSQWYNFFNFWQLTGKKDDN"
     gene            9269..10804
                     /locus_tag="VF_0852"
                     /old_locus_tag="VF0852"
     CDS             9269..10804
                     /EC_number="4.3.1.3"
                     /codon_start=1
                     /locus_tag="VF_0852"
                     /old_locus_tag="VF0852"
                     /product="phenylalanine and histidine ammonia-lyase"
                     /protein_id="AAW85347.1"
                     /transl_table=11
                     /translation="MTTNSITFGKGRLTIEDVVDIANGANAQLNNSDEFTAKIDRGVEF
                     LERLLKEEGVIYGVTTGYGDSCTVAIPMDLVDELPLHLTRFHGCGLGENLDEQQARAVL
                     ATRLCSLSQGVSGVTHDLLNQIVTLINHGVSPRIPQEGSVGASGDLTPLSYLAAALIGE
                     REVIYKGEIRATADVFKELNITPIKLKPKEGLALMNGTSVMTALACLAYKRAEYLAQMA
                     TKITAMVSVGMQGNDFHFDEALFAVKPHPGQQQIAAWLRSDLHNETPPRNSDRLQDRYS
                     LRCAPHVIGVLQDSLPFLRQMIENELNSANDNPIIDGDNERVLHGGHFYGGHIAMAMDM
                     LKTAVANIADLLDRQMAQLMDYKFNNGLPFNLTGSTGARKPINHGFKAVQIGISAWTAE
                     ALKGTMPASVFSRSTECHNQDKVSMGTIASRDCLRVLQLTEQVTSASLLAATQALVLRE
                     KQGELDDTQLSKELKTMRDAVLSEFAFVDEDRPLENDLRNFMAKIQQQHWTLY"
     gene            10816..11262
                     /locus_tag="VF_0853"
                     /old_locus_tag="VF0853"
     CDS             10816..11262
                     /EC_number="3.-.-.-"
                     /codon_start=1
                     /locus_tag="VF_0853"
                     /old_locus_tag="VF0853"
                     /product="esterase"
                     /protein_id="AAW85348.1"
                     /transl_table=11
                     /translation="MTNTNEAKPVNVLSAEVMITTAFQDADPMGVVYHGNYFRFFEKAR
                     HEMLEKIGYSYRDMMASGYVWPIIDTRVKYVKSIPYDHKIRVEATLTEWENRMRVDYVI
                     YDAETNVRMTKAHTMQVAVSIETEEMCFVSPRIFTDKVEAYHNA"
     gene            11255..11875
                     /locus_tag="VF_0854"
                     /old_locus_tag="VF0854"
     CDS             11255..11875
                     /codon_start=1
                     /locus_tag="VF_0854"
                     /old_locus_tag="VF0854"
                     /product="hypothetical protein"
                     /protein_id="AAW85349.1"
                     /transl_table=11
                     /translation="MLNVKRYWSLLLASFVMLFSVNSFAFSVDDLQQQLAKSTLVRGDF
                     KQVRTMQMFAQPLSTSGTFLLDHEKGLLWQQTTPFPIALTLTQNKLRQSINGDTQVMTD
                     SDNPMAFYFTRLFLSLFKGDTESIKENFTLQLTGEKDAWTLILTPTKAPIDSVFKTIKI
                     EGNDYLNRVVLSEVRGDVTEMVFTNQSTEPATLTEEELRVFEF"
     gene            12397..14292
                     /locus_tag="VF_0855"
                     /old_locus_tag="VF0855"
     CDS             12397..14292
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1035:MMPL domain-containing transport protein (Score:
                     53.2; E-value: 2.5e-16)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="VF_0855"
                     /old_locus_tag="VF0855"
                     /product="transporter"
                     /protein_id="AAW85350.1"
                     /transl_table=11
                     /translation="MTGGELENDPFLLFRDYLSGLTQSSGNVQVENGYLIAKHDDKEYV
                     VITADLSGSSYQLSLQQQLPQLLSLESKVSKTFSVDIQHTGVIFYAAHGTESAKGEIST
                     IGLGSLIGIIVLVFLVYRSTLPLALALLSISTGLACAYVLTIALFGSIHLFSLVFGASL
                     IGVSIDYAFHFLTDRLHAGKKWNSVVGIQQLFPAITLGLITSLIGYLGMLIAPFPGLQQ
                     LSLFSAIGLTAAYFTVVCWYPILASKPNQYNKVPMQRALSQWLLVWQQPLIRLVLPLSL
                     LIAASFGVYMAKYDDDIRQLQALPHALQQQEREIKAITGVSGSQQLLLVRDSSEEGLLQ
                     KLETISTKLDKRNDFGGYQSISRYVPSQKRQQENYQLVKHLYQQQSEYYQSVIQQKTAL
                     DFSQQYQPLTIDGFLASPISDTVGFLWLGKIDDVFASVIAFNQEVNSTELQAFAADNHI
                     TYLNKAEEISRLFADYRHRITELLLFAYAAIFLLLLPRYKMKQAFLIVLPPFIAGCAGL
                     AVTVLTGVPLNLFNLLALMLILGIGIDYTLFFAEQNKQSDDSHKESTLLAISLSALTTI
                     LSFGLLALSETQAIHSFGITVLTGIIVAWLLAPLSQQNKESKGYINTLSEKKDAS"
     gene            14282..14908
                     /locus_tag="VF_0856"
                     /old_locus_tag="VF0856"
     CDS             14282..14908
                     /EC_number="2.7.8.13"
                     /codon_start=1
                     /locus_tag="VF_0856"
                     /old_locus_tag="VF0856"
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="AAW85351.1"
                     /transl_table=11
                     /translation="MHHKAIKVRHLIALVLSFLLMGCAAKPVKQPNQVEVSQGQFITLP
                     QPEEYGSDLSLSQLISVEWQGKKNQLPVQLQLTGNDLVLAGFASWGSRLLSLDYDGQTL
                     DTYVMAGLSDTLPPPEQVLFNVMITLWPLEAWQESLTSIGWQLNEHDNHRTLVTESGKT
                     ILDARYDDITDKLSGEIVLTSPDFGYVIKIKTFSHSNTTTQNNNG"
     gene            14914..16101
                     /locus_tag="VF_0857"
                     /old_locus_tag="VF0857"
     CDS             14914..16101
                     /EC_number="2.3.1.41"
                     /NRPS_PKS="Domain: PKS_KS(Iterative-KS) (89-391). E-value:
                     2.1e-35. Score: 114.3. Matches aSDomain:
                     nrpspksdomains_VF_0857_PKS_KS.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     arylpolyene: APE_KS2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1022:Beta-ketoacyl synthase (Score: 194.1; E-value:
                     6.9e-59)"
                     /gene_kind="biosynthetic"
                     /locus_tag="VF_0857"
                     /old_locus_tag="VF0857"
                     /product="3-oxoacyl-(acyl carrier protein) synthase"
                     /protein_id="AAW85352.1"
                     /sec_met_domain="APE_KS2 (E-value: 1.2e-172, bitscore:
                     564.6, seeds: 16, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDFPLYIHACGSHSAMGKEVDEIHQVLSGNKAPSMHKETGWLNN
                     GKATVVGKALGELPLVPEHLAHWDTRNNQLVLSALQQIEPQINAAKEKYGSDRIAVIVG
                     TSTSGISDGEVALKEKMEQGAFPAGYSYKQQELGNPADFIAQYLDINGPTYAISTACSS
                     SGRVFLSAKRLLKAGFVDAVIVGGTDSLCRLTLNGFNSLEALSNEYCLPFSPNRKGINI
                     GEASAYMLLSAEQKDSDDPIALLGAGDSSDAHHISAPHPEGKGAIEAMNKALTDAGLEP
                     KDIGYINAHGTATPLNDAMESKAVHAVFGHSVPVSSTKPFTGHTLGAAGIIEASICWHI
                     LKYNLPLPKQINDGQQDDALADIQLVVDQPLRKKAVISNSFAFGGNNISLIFGCI"
     aSDomain        15181..16086
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_VF_0857_PKS_KS.1"
                     /domain_subtypes="Iterative-KS"
                     /evalue="2.10E-35"
                     /label="VF_0857_PKS_KS.1"
                     /locus_tag="VF_0857"
                     /protein_end="391"
                     /protein_start="89"
                     /score="114.3"
                     /tool="antismash"
                     /translation="AKEKYGSDRIAVIVGTSTSGISDGEVALKEKMEQGAFPAGYSYKQ
                     QELGNPADFIAQYLDINGPTYAISTACSSSGRVFLSAKRLLKAGFVDAVIVGGTDSLCR
                     LTLNGFNSLEALSNEYCLPFSPNRKGINIGEASAYMLLSAEQKDSDDPIALLGAGDSSD
                     AHHISAPHPEGKGAIEAMNKALTDAGLEPKDIGYINAHGTATPLNDAMESKAVHAVFGH
                     SVPVSSTKPFTGHTLGAAGIIEASICWHILKYNLPLPKQINDGQQDDALADIQLVVDQP
                     LRKKAVISNSFAFGGNNISLI"
     CDS_motif       15367..15405
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_VF_0857_0001"
                     /evalue="4.90E-04"
                     /label="PKSI-KS_m3"
                     /locus_tag="VF_0857"
                     /protein_end="164"
                     /protein_start="151"
                     /score="12.7"
                     /tool="antismash"
                     /translation="GPTYAISTACSSS"
     gene            16092..16562
                     /locus_tag="VF_0858"
                     /old_locus_tag="VF0858"
     CDS             16092..16562
                     /codon_start=1
                     /locus_tag="VF_0858"
                     /old_locus_tag="VF0858"
                     /product="predicted 3-hydroxylacyl-(acyl carrier protein)
                     dehydratase"
                     /protein_id="AAW85353.1"
                     /transl_table=11
                     /translation="MYLMSSIPPLIDLLPHDTPLALVDELIEVNDLSVHCQVTIGENNV
                     FFDHETKTVPAWVGIEFMAQSIAGWSGYHAWKKGNKSPIGFLLGSRKYQAECSEFQQGD
                     TLDIFAEQVLENNGMAVFSCYIEHKGQQLASSQLNVFVPTEEKLEELLTPKQ"
     gene            16582..17307
                     /locus_tag="VF_0859"
                     /old_locus_tag="VF0859"
     CDS             16582..17307
                     /EC_number="1.1.1.100"
                     /NRPS_PKS="Domain: PKS_KR (4-159). E-value: 1.4e-20. Score:
                     65.8. Matches aSDomain: nrpspksdomains_VF_0859_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001:short-chain dehydrogenase/reductase SDR (Score:
                     215.5; E-value: 1.3e-65)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="VF_0859"
                     /old_locus_tag="VF0859"
                     /product="3-oxoacyl-(acyl-carrier-protein) reductase,
                     putative"
                     /protein_id="AAW85354.1"
                     /sec_met_domain="adh_short (E-value: 5.5e-39, bitscore:
                     124.4, seeds: 230, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKRQVLVTGASKGIGRAISEQLAKDGFYIVVHYMSDKAGAETTLA
                     AIQEHGGEGRLIQFDISDRAQCKATLESDIETHGAYYGVVSNAGITRDTAFPAMTEEEW
                     DGVIHTNLDSFYNVLHPCVMPMVQKRKGGRIVTLASVSGIMGNRGQTNYSAAKAGVIGA
                     TKSLALELAKRKITVNCVAPGLIDTGMVDEHVKEHALPQVPLRRMGEPEEVAGLVSYLM
                     SDVAGYVTRQVISINGGLV"
     aSDomain        16594..17058
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_VF_0859_PKS_KR.1"
                     /evalue="1.40E-20"
                     /label="VF_0859_PKS_KR.1"
                     /locus_tag="VF_0859"
                     /protein_end="159"
                     /protein_start="4"
                     /score="65.8"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="VLVTGASKGIGRAISEQLAKDGFYIVVHYMSDKAGAETTLAAIQE
                     HGGEGRLIQFDISDRAQCKATLESDIETHGAYYGVVSNAGITRDTAFPAMTEEEWDGVI
                     HTNLDSFYNVLHPCVMPMVQKRKGGRIVTLASVSGIMGNRGQTNYSAAKAG"
     CDS_motif       16597..16653
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_VF_0859_0001"
                     /evalue="2.80E-04"
                     /label="PKSI-KR_m1"
                     /locus_tag="VF_0859"
                     /protein_end="24"
                     /protein_start="5"
                     /score="13.6"
                     /tool="antismash"
                     /translation="LVTGASKGIGRAISEQLAK"
     CDS_motif       16987..17049
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_VF_0859_0002"
                     /evalue="1.30E-08"
                     /label="PKSI-KR_m4"
                     /locus_tag="VF_0859"
                     /protein_end="156"
                     /protein_start="135"
                     /score="27.3"
                     /tool="antismash"
                     /translation="VTLASVSGIMGNRGQTNYSAA"
     gene            17304..18539
                     /locus_tag="VF_0860"
                     /old_locus_tag="VF0860"
     CDS             17304..18539
                     /EC_number="2.3.1.41"
                     /NRPS_PKS="Domain: PKS_KS(Iterative-KS) (82-406). E-value:
                     3.8e-43. Score: 139.8. Matches aSDomain:
                     nrpspksdomains_VF_0860_PKS_KS.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     arylpolyene: APE_KS1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1022:Beta-ketoacyl synthase (Score: 301.3; E-value:
                     1.9e-91)"
                     /gene_kind="biosynthetic"
                     /locus_tag="VF_0860"
                     /old_locus_tag="VF0860"
                     /product="3-oxoacyl-(acyl carrier protein) synthase"
                     /protein_id="AAW85355.1"
                     /sec_met_domain="APE_KS1 (E-value: 5.6e-210, bitscore:
                     687.5, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSRRVVVTGMSGVTALGSDWNTVGENLRKCENATQYMPSFEVYDG
                     LNTKLAAPINDFTLPKHYTRKKIRSMGRVSLLSTVASEQALEQSGLIGNDILTNGQTGI
                     AFGSSTGSTDAVGAFGVMLNEKSTRAITATTYVQMMPHTAAVNVGLFFGLRGRVIPTSS
                     ACTSGSQAIGYAYEAIKHGYQTAMVAGGAEELCPTESAVFDTLFATSLKNDAPKTSPSP
                     YDQDRDGLVIGEGAGALVLEEYEHAKARGATIYAELVGFASNCDAAHVTQPQMETMQMC
                     MEMSLRDANLAPEKIGYVSAHGTATERGDIAESTATENVFGSSMPISSLKSYFGHTLGA
                     CGAIEAWLSIEMMRNNWFSPTLNLNNVDERCGKLDYITGTGREIECDYIMSNNFAFGGI
                     NTSLIFKRWTEE"
     aSDomain        17550..18521
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_VF_0860_PKS_KS.1"
                     /domain_subtypes="Iterative-KS"
                     /evalue="3.80E-43"
                     /label="VF_0860_PKS_KS.1"
                     /locus_tag="VF_0860"
                     /protein_end="406"
                     /protein_start="82"
                     /score="139.8"
                     /tool="antismash"
                     /translation="EQALEQSGLIGNDILTNGQTGIAFGSSTGSTDAVGAFGVMLNEKS
                     TRAITATTYVQMMPHTAAVNVGLFFGLRGRVIPTSSACTSGSQAIGYAYEAIKHGYQTA
                     MVAGGAEELCPTESAVFDTLFATSLKNDAPKTSPSPYDQDRDGLVIGEGAGALVLEEYE
                     HAKARGATIYAELVGFASNCDAAHVTQPQMETMQMCMEMSLRDANLAPEKIGYVSAHGT
                     ATERGDIAESTATENVFGSSMPISSLKSYFGHTLGACGAIEAWLSIEMMRNNWFSPTLN
                     LNNVDERCGKLDYITGTGREIECDYIMSNNFAFGGINTSLIFK"
ORIGIN
        1 ttatggctta cataattcag caaccacttt taatcgacgc tctggctcgg caacaaatgg
       61 attgctttca tcccaagcat agccagctaa aatggcgcag atcatttgtt taattttagg
      121 attcggattt tcatggaaga tcacatcttg gaatgtccct tcataccatc cttctacgta
      181 acaacgaaaa gcattgactc ctttcatcaa cttatctgag taatcttgct gccaattaac
      241 gtcttcttga ttaaattgct taatcaataa gtctgcagct agctctgccg atttcattgc
      301 aatcgtgaca ccagaagaga acaccggatc taaaaattca ccagcattgc ccagtaaagc
      361 aaagttttta tcaaacagct tactgacatt ggcagaatag cccatgattt caccgcaagg
      421 gttcgggtat tcagcttggt ttaacagatg tttgagatta ctttcttctt ctgcaagttg
      481 ttttaatgcc gttactttat cttctgaata tgcatcgaag aactcaggct ctccaaccac
      541 accaaaagag caggtcccat tactgaatgg aattaaccaa taccacactt gtgtatttgt
      601 tggatgaaca gagactaaaa ttttatttct gtcgtagctt tcgtcagtaa tattgtcttt
      661 tatatgtgta aaaatagcac ggcgtggtgg cagacatgag ggctcttcta attccaataa
      721 ttttggtaga acacggccaa agccactcgc atcaagaatg aaatctgctt gaatttggta
      781 aggcgttccg ttttcatcca gtaccgataa tagaggcttt tctgcattta catccacatc
      841 ggttatttca tgctgatagc gaatttcaac gccttgttct tttgcacaat cagctaaaac
      901 tttatcaaag ttgccacgtt gtacttggaa tgtggttcct gcgccgtcgg tgaatttgtc
      961 cgtaaaatca aaagaagtga aagtgccatt acaatgaaat gcagcaccat ctttgaattg
     1021 aaaacaagca tcttcaacag cgtttaacat gcctgcatgt ttaatggatt ccatacatgc
     1081 aggtaataaa ctttcaccga tagaaaagcg agggaaagtc gatttttcaa gaataatgca
     1141 gttgatattg tgtttgttta atagggcagc agcaatagag ccagaaggtc cagcaccaat
     1201 aatagccact tgggttgttt gtggtgataa cacaagtgct ccttagagac aaaagcacca
     1261 ctttaggtat cagtaaccta aagtggtgag gatattatta tatttataag tgatttttat
     1321 ttaaaaacag acttacttag cacgaagttc aagtaatgtg tggcctaagc caatattgtc
     1381 tgtttgctca gcaacagcaa aacccgcttc ttcaataatt tctaagaaat cactacaacg
     1441 atagaaacga ctattaccat tggctaaaca agtgaagtat agagaggttg catttaggct
     1501 aaacgaagca gcatcgtatt tttgcgcatc ccaaaatagc tctaaaatat acactttcgc
     1561 atcggtagac atggctgcac gtacacgttt taaaatgctt aaaatttcca ttggagagaa
     1621 acaatctagg aattggctca tccaccatac gtctgcgcct tgaggtaatg gtagagtctt
     1681 atcaagcata ttagttgggt gaccattaat acggtcggca aaaccttgtt ctttggcatt
     1741 ttttaacgcc atttcgattt gttgtggaag gtcaatgatt gttacttcaa catcgttatc
     1801 gtaattacag cattgtagtg cccatttacc cgtgttgccg ccgatatcaa acaaacgctt
     1861 tggtttttgg cttaatactt tttctaataa gattgggaac gaacggtcag aatagaagtg
     1921 atcaaactta aaccagctct ctttggcttt ttctggtaat cgagacaaac cttcataaat
     1981 cgttggccag tcacctaact cttttaagcc ggcaggtgtg ccttcttgaa ttgcctctgt
     2041 aagatgcatc atagcggcat aacaaacatc agctgtaaag tctaagttag cgttcgtcat
     2101 cccatcgtgt aataagaagt gcccaaggtt cgcaagaaca tagtttggtt tattccaagt
     2161 aacaatgtgc gcacttaatg ccatatcaag taagactttc acgccatatt cagatacacc
     2221 cgtttcttgt gctaattgct cagcatctaa accttgatct tgagctttat ctaaagccgc
     2281 tagaatacca agatctctaa gcgttcgagc tgtatgaaaa acaataggag caaaagaaag
     2341 tttttgagct tctgtttttg cttctaatgc gttaaatggg tcatttttat attgagtcac
     2401 agagattaac catagttgaa gataataaaa tgatcatatc agtggtattg agtgaaactt
     2461 tgttcaaaag caagaaacca ctgatatata agagagaatg atattaatcg attatatttg
     2521 atacggtatt accgttaaat tcgactttaa aagtattaaa tctatttttc caatataacc
     2581 aatgatcgct atttaaatta ggtgttctat gttctggtgg gaagccgatg gcatatttta
     2641 cgccttcttt tgtcatgcct ttcttaacca caccttcttt tattccctgc tgatcaagtg
     2701 cacttagttt ttctactttt tcagtattac actcagtacc gaaatagttg cttaagtatt
     2761 ttgcaaaatc aggagatgat ttgtgtttat ctaaaatata ttctctgcca ctactatcaa
     2821 ttttaaatgt aagacgttta gtactaacgt tgacaatatc aacttgagta cagagaggga
     2881 ttaatccatc ttgttgaaaa ttaacagaat aaagttttga gcgagtgtcg tcagggtgaa
     2941 ggttaacaag agtataaacg ggtttattgg taagaaataa atcgtcaccc gtatttttgt
     3001 cagtaagagt catgacactc gcgcaacccg ataaaattat taaaccggaa gtcgctaata
     3061 atggttttaa aaatgacatg gtagtccttt aaatttcgct atatatattt atttaccgta
     3121 tgactcgcat taatctattt gcgccaagtc actgaattca tatatcctat acgagtaggc
     3181 taacaaattg taatattttt acgactagat ggatatcatg acatacgcaa attttaatat
     3241 tatcgattgg cacgcaattt ctgcaggttt ggatagcaaa aatgcgtggt tagaatggcg
     3301 tgaaaataat ttcacttggc ctgaaaatga aaaagcgcca gttgataaaa taccagcaat
     3361 ggctcgaaga agaatgagct cgttaagtaa attagccgtg caaaccgcat taacattagc
     3421 ggaagaaaat gacattgatt atttggtgtt ctctagtcaa catggtgagt taactcgaac
     3481 tgtcgctctt ttagaagata ttttgaatgg agatgatgct tctccaatgg ctttttctca
     3541 atcggttcat aatactgccg caggtctatt taccatcacg gccaaaaaag ccattccagt
     3601 tacttctatc gctgctgggg aaaatacctt tcattctgcg gttgttgaag ccgctgcgta
     3661 cttagctgaa aatcaaaata acaaagttct cattatcgac tttgacgagc ctttaccttc
     3721 tcattaccaa gagtttgaaa aacaaaacta tcaaggctat gcagttggct taataatgag
     3781 cgctggtgac attttttctc ttcaatggaa caataaacct acaattaaac cttctggatt
     3841 gcctcatggg ttgcaatgta tagctaactt agtaggatca cactctcaat ggataacgga
     3901 gagttcttca aaacaatggg tatggaatcg aaaatagtct tatgacttta cttcaacgca
     3961 ctgataaata ctggcgaatt ttagctactg ggttctgttt ctctttattc ggagtcggtg
     4021 cactttgctt gacgtttatt gtttttccat tacttacatt tactgtaaaa gataaaaaac
     4081 aacgagaagt tcgagtgcaa tcgattatcc aacgtagttt ttttatcttt tgtaaaacaa
     4141 tgcactatag tcgtgcgatt aagtatcaat ttgatggtgc agagcttctt cagagtgaca
     4201 gaaattgctt aattgtcgct aatcacccaa gcttaataga ttatgtttta attgcttcac
     4261 aattgaagca atgcgattgc atcgtaaaag cagccttatg gcataatccg tttacacaag
     4321 ggattgtcag agctgcgggg tacattccaa atcaagaacc agaagcgttt ttagaagagt
     4381 gttctgaaaa attaaactca ggtaatgtat tgttggtttt tcctgaagga acgcgaacaa
     4441 caccgggtaa aaaatcaaaa cttcaacgag gtgcagcgca aattgcggtt cgtagtcaaa
     4501 gtgatattcg tattattcat atcacagtaa cacccagctt tttaactaaa gaagcaaagt
     4561 ggtatcaagt tcctgatgtt cgccctttct ttcatttaca agtgaaagag agagtcgcca
     4621 tcactccatt tatcgaagac gctgactctt catcttcagc ggctcgaaat gtaaatcgat
     4681 ttttagatac agctttgtat ccacaaaatg aagtcaacac atcaaaagaa aataagtagg
     4741 ccattgatgg aagtattaca caacgaaatt aaacaattaa ttattgacgc tcttaaccta
     4801 gaagatatct ctgttgatga tattgaaacc gaggcaccgc tatttggtga cggcttgggc
     4861 ttagattcta tcgatgctct agagttaggt ttagcaatta agaaaaagta caacttagtg
     4921 ttagatgctg acgatactca aactcgtgaa cattttgcgt cagtagcaaa cctagccgct
     4981 tacattaatg caaacaaagc gtaagagagt aaataatgac agacgtaaat aaagagcaag
     5041 tattagagca agtaacttct gcattagttg aactgtttga aattgatgaa gcagatatta
     5101 ccccagaagc tcagctgtat actgaccttg atttagatag tatcgatgct gtcgatcttg
     5161 ttgttcattt gcagaaagtg acgggtaaaa aaatcaaacc agaagagttc aaagaagttc
     5221 gtactgttga cgatgtagtt aattctgtag ctgcactgtt aaaaggcgaa taatgcgttg
     5281 gctgacagcg ctgtcagcca ttgcactctt agcatatccg ctagcggtct actatggtct
     5341 ccattttggt ggaataaaga cagtagcagg cgtactagcg gtgttgtttt tagtgcgtat
     5401 ggtcggtggt aaacaagtac cgataaaaga actcaaatac atcgctatta tcagtggtgg
     5461 agcagggata attcttgctt ctcttggttg gatattcaaa accgaaggct ggtttacctt
     5521 ctatcctgta atagtcaatg tcgttatgtt cgttatgttt ttttggagtt tatttcaatc
     5581 ccaaagcatg attgaacgtt ttgctcgtct acaagaacct gatttacctg aaagtggcgt
     5641 taagtacacc agaaccgtaa ctaaaatttg gtgtgtattt tttattatta atggctctat
     5701 ctctttttct acctgtttca tggatatgaa gatgtggatg ctatacaacg gatttattag
     5761 ctacttgctt attggaactt tgttcataag cgaatggtta gtgcgccaat ggataagaaa
     5821 aaaagaagta tgaaaacagt ctctctttcg caattagtat tacaagataa aacagaccac
     5881 acggtgtgtt ttgatgagca gcactcatat atttcttatt ctcaatttca aaaagacgtt
     5941 cagaaagcgt gtgccttttt tcagcagcaa cctcacacta agtgggcatt gtgttttgat
     6001 gacagctatt atttttcggt tacttttctt gcattagccc atgcgggtaa acaccttatt
     6061 cttctaggta atcatcaacc agcggcattg gccgaattat ctcagcattt tgatgcctta
     6121 attcatgatg acattattaa agtgatgcca tgcagtgagc atgttgccta tcatgatata
     6181 aaaagtgacc atttagctac attatcacca cttcctcttc atgaggttga gcttacttta
     6241 tttacttctg gctcaactga tacacccaag cccattgcta aaaaccttca attacttgat
     6301 aacgaaattc aacaattaga ctgtttatgg ggtgaaaaac tcggacaaag tgacatttac
     6361 agcaccgtat cacaccaaca tatctatggt ttattattta gagtgttgtg gccattaacg
     6421 gctggtcgtg cctttagtcg ccataacttt atctacccag agcagatttt atctcatcga
     6481 ccagatgata aaaaaacgct tatttctagt cccgcattgc ttaagcgctt ggttgatcac
     6541 gaagggtaca atggctatca gatggttttc tcttccggcg gacctcttgc tattgaaggg
     6601 gctgagcaaa gtgagaaaaa actctcactg cgtccatttg aagtttatgg tagtacagaa
     6661 acaggtggta ttggctaccg tcagcagttt gaaccaagct ctttatggtg cttctttcct
     6721 tctcatcaag ctcgtttaaa cattgaacat tgcttagaat tgctttctcc atttattgat
     6781 gacgaagagt ggtacgcgac gagcgattac tgtgatttgc ttgctaatgg tcagtttgtt
     6841 ctaaaaggtc gcgtcgatcg cgtgatcaaa attgaagaaa agcgcctctc tttaacggaa
     6901 gttgaaaaac gattaatgca gttagagtgg attaatgaag cggcagtgat cccgatggaa
     6961 gaagaaaaac gcaccgtatt gggcgcagtg attgttctat cagaagcagg aactaaacta
     7021 atggagcagg gctctaaggg taagttttgg ttgagattac gctcagaatt acgtgaatgg
     7081 ttagagcctg ttggtattcc tcgtcgtttt agaactgtta gtgaaattcc attaaatagc
     7141 caaggtaaac gtcaaatgca cgatttaaag cagctattta atgatgcgaa taattaaaaa
     7201 agattagatt atgataaaac gtaagccaag tatccttaca acaaaagata tctcagtaaa
     7261 tgaagcgcaa ccaagtgtgc ttatttgtat gcgtgtggat gatgatatct tagacttcac
     7321 cggacacttt ccaaatcatc ctttgttacc cggtgttact caagttgatt gggctattca
     7381 ttatgcgaaa cattacctta atgcgcattc aaactttaaa ggaatggaag tacttaagtt
     7441 tcaagaacct attttaccga atatggaaat agaacttgag cttaaatggg atattgagaa
     7501 gagtaagctg tattttactt accgctcaaa taaaggcgat acggtaagca atcatgcttc
     7561 tggtcgtatt gtgttaggag cgtaatcatg actaactttc gcccttgttt tttaattcct
     7621 tgttataacc atggtaaaac cattcctgcc gttgttgaat cattgatgtc gtatggttat
     7681 ccaatgatca tcgttgatga tggcagtgag aatgaaacaa agcgtatttt agaagaagta
     7741 actcaacata atgagtcaat taccttaatc acattggctg aaaaccaagg taaaggtggg
     7801 gctgtgattg ctggtatcga aaaagcctat cagcagagtt acagtcatgc gattcaaatt
     7861 gatgctgatg gtcaacatga tctcgaagcg ctacctaaat taatcgctga atctcaagaa
     7921 cacccaacgg cactgatttc tggacagcct atttacgatg agagtgtacc taaatctcgt
     7981 ttgtatggtc gctatgctac gcatatttgg gtgtggattg agacactttc ttttaccatt
     8041 aaagacagta tgtgtggttt ccgctcttat cctatcggtc caacgattaa cgtattagag
     8101 cgagcggtgc ttggccgacg aatggatttt gataccgaaa tcatggttcg tatgtactgg
     8161 aatgaaaccg atattcgctt catcaatact cgtgttattt acccagagga tggcatttct
     8221 cattttgatg cattatggga taacgtgaaa atcagttgga tgcataccaa attatttttt
     8281 ggcatgctgc ctcgtattcc gtcattacta aaacgtaaac ctgttcaaga agatcattgg
     8341 tcgtctcatg ctgagcgcgg tacgatttta ggtattcaat ttttactttg gatctattca
     8401 acgtttggtc gcaaggtatt ttcactatta ctcaaaccag tcatggctta ttactaccta
     8461 cgtggtggtt cagtcaaaga cgcttctgat gatttcattc aacaggtaaa tgcttatgct
     8521 cgaattaaag gcattcaatt acctgaaaag ctaaccactt atcgtcattt ggtctctttt
     8581 ggtgaaacca tgttagataa gcttgcggca tggcgtggtg atttttcaga taaaaactta
     8641 acggttcatg gtattgagca ttatcaaaca ttagctcagc gtgaaaaagg cattgtatta
     8701 ttgggttctc atttaggaaa tctagaacta tgtcgtgcgc ttagccgtcg tcatactcac
     8761 ctgaagatta atgcgttagt ttttactgag catgcagagc gttttaatac ggtaatgaaa
     8821 accgttaatc cgcaatcaga agtgaatgtt attcaggtgt ctaaaatagg gccagatacc
     8881 gccattttat tgcagcaaaa aattgatgat ggtgagtgga ttgttatcgt tggtgataga
     8941 acgtcagtca ctaaagagaa tcgagtgatt tgggctgatt ttcttggtaa gcctgcgcca
     9001 tttcctcaag ggccatttat gttggcctct attttaaagc aaccagtata tttaatgttt
     9061 gggcttcgtg acgatacaca aaaagatccg ttgtttgatg tgtatttaga accgttttct
     9121 gaacaaatca tattacctcg tggtaagcga gaagaggctt tgcaagaagt cgtacaaaat
     9181 tatgctcaac gtctagagca tttcacatta aaagcaccat cacaatggta taactttttt
     9241 aatttttggc agttaactgg aaagaaagat gacaactaat tcaattacat ttggtaaagg
     9301 acgtctgaca attgaagacg ttgttgatat tgcaaatggt gcaaatgcac aacttaataa
     9361 ttctgatgaa ttcactgcca aaattgaccg tggtgttgag tttttagaac gtttattaaa
     9421 agaagaaggc gtgatctatg gtgtaacaac gggttatggt gactcttgta ctgtagcaat
     9481 cccaatggat ttagtggatg aattaccgct gcatttaact cgttttcacg gttgtggttt
     9541 aggcgaaaac ttagatgaac aacaagcacg tgctgtatta gcgactcgct tatgctcttt
     9601 atcacaaggg gtatcgggtg tgactcatga tctgcttaat caaatcgtga ctctaattaa
     9661 tcacggcgta tctccacgta ttcctcaaga aggttctgtg ggtgcaagtg gtgatttaac
     9721 gccattatct tatcttgctg ctgcccttat tggtgagcgt gaagtgattt ataaaggtga
     9781 aatccgtgca actgcagatg tatttaaaga gctaaatatc acaccaatca agcttaaacc
     9841 aaaagaaggt ttggctttaa tgaatggtac atcagtgatg acagcgttag cgtgtttagc
     9901 atataagcgt gctgagtact tagcgcaaat ggcaactaag atcaccgcaa tggtatcggt
     9961 agggatgcaa ggtaatgact tccactttga tgaagcgtta tttgcggtga aaccacatcc
    10021 aggccaacaa caaattgctg cttggttacg ttctgattta cataacgaaa caccaccaag
    10081 aaacagtgat cgtctgcaag accgttactc acttcgttgt gctccacatg tgattggtgt
    10141 acttcaagat agcctaccgt tcttgcgtca aatgattgaa aatgaattaa acagtgcgaa
    10201 cgataaccca attatcgatg gtgataatga gcgtgttctt cacggtggtc atttctatgg
    10261 tggtcatatt gcaatggcaa tggacatgct aaaaaccgca gtagcaaata ttgctgattt
    10321 attagatcgc caaatggcac agctaatgga ttacaaattc aataacggtt taccatttaa
    10381 cctgacgggg tcaacagggg ctcgtaaacc aattaaccat ggctttaaag cggttcagat
    10441 tggtatttca gcatggacag cagaagcgct taaaggaaca atgcctgcaa gtgttttctc
    10501 acgttcaaca gaatgtcata accaagataa agtgagcatg ggtacgattg cctctcgtga
    10561 ttgtttacgt gttttacagt taacagagca agtgacatca gcttcattat tagcggcaac
    10621 tcaagcgtta gtattgcgtg aaaagcaagg tgagcttgat gatactcagt taagtaaaga
    10681 gcttaaaacc atgcgtgatg cggtattaag tgaatttgct tttgtcgatg aagaccgacc
    10741 attagagaac gatttacgta actttatggc gaagattcag caacagcact ggacactata
    10801 ctaaggttaa atgttgtgac taatacaaat gaagctaagc cagtgaatgt attaagcgca
    10861 gaagtgatga tcacaaccgc ctttcaggat gcggatccga tgggagttgt gtatcacggt
    10921 aattatttcc gattttttga aaaagcacgt catgaaatgc ttgaaaaaat cggttatagc
    10981 taccgagata tgatggcgtc aggctatgtt tggcccatca ttgacactcg tgttaagtac
    11041 gttaaatcca ttccttacga tcataagatc cgagtggaag cgacactaac ggaatgggaa
    11101 aaccgcatgc gtgttgatta tgttatctat gatgcagaaa ccaacgtacg aatgactaaa
    11161 gcccatacaa tgcaagtagc ggtaagcata gaaactgaag aaatgtgctt tgtttctcct
    11221 cgtatattta ccgataaagt agaggcttat cataatgctt aatgttaaac gttattggtc
    11281 attattattg gcatcgtttg ttatgctttt tagcgtaaat agctttgcat ttagtgttga
    11341 tgacttgcag cagcaactcg caaaatcgac gcttgttcgt ggtgatttta aacaagtaag
    11401 aaccatgcag atgtttgcac aaccattatc gacatcaggt acttttttgt tggatcatga
    11461 aaaggggtta ttgtggcaac aaacaacgcc ttttccgatt gcgttgactt taacgcagaa
    11521 caaacttcgc caatccatta atggtgacac tcaagtcatg acggattcag ataatccaat
    11581 ggcgttttat tttactcgtc tatttctatc tctatttaaa ggggatacgg aatccattaa
    11641 agaaaatttc actctgcaat taacgggtga aaaagacgct tggacactca tacttactcc
    11701 aactaaagcc ccaattgaca gcgtatttaa aaccataaaa attgagggta atgattattt
    11761 aaatcgagtg gtattaagtg aagtgcgagg tgacgtaact gaaatggtat ttactaatca
    11821 gtcgacagaa cctgcgacct taacagaaga agagttacgt gttttcgaat tctaattgga
    11881 taaaaccacg agttttagct ctggtttggg cattggctct attaggaatc gcaggggtat
    11941 ttggttatca atgtacaacc aaaggcgcac tgccaataga aaccaatatc ttggcgttat
    12001 tacctgataa tcaacaagat acagttgctc agcaaagcgt ttgataatat tgctaatacc
    12061 atgagtgata aagtgggtgt tgtaagttag gccaaaaccc caaggttgga caagttaaaa
    12121 aactggattt gaaagcgggt ggattccagg tttaatggaa caggaacttg gtttgcgttt
    12181 accccttatt ggttttgacc tcgggtaaac gggcaaacga attaagttcc cttgaagcaa
    12241 caaacaaggc ggtgggggga caacttttat ttttcctggc ttcgcgctca gtttacttga
    12301 gcagcaacaa ggcaaaaccc aaacaattta gaaacacgca accagaagca gcaagttcaa
    12361 aaggtattac acgcactata caatcctttt tcaggtgtga cgggagggga gttagagaac
    12421 gatcctttct tgttatttcg agattattta tcagggttaa ctcaaagctc tggtaatgtt
    12481 caagtagaga atggctatct gattgccaag cacgatgata aagaatatgt cgtgatcacc
    12541 gctgatctat caggatcatc ataccaactc tctttgcagc agcaattacc tcaactattg
    12601 tcacttgaaa gcaaagtatc aaagacattt tcggttgata ttcaacatac aggtgtgatc
    12661 ttttatgcag cccatgggac agaaagcgcc aaaggggaga tcagcacaat cggccttgga
    12721 tcccttatcg gaattattgt ccttgtcttt ttagtttatc gcagcacatt acctttggct
    12781 ctagcattac tgtcaatatc aacgggtctt gcttgtgcct atgtactgac gattgcttta
    12841 tttggttcca ttcatttgtt tagtttggtt tttggtgcca gcttaattgg ggtttctatt
    12901 gattatgcat ttcacttttt aaccgaccga cttcatgcgg gcaaaaaatg gaattcagtg
    12961 gtgggtattc agcaactgtt tccagccatt accttggggc taattaccag tttaattggt
    13021 tatttaggga tgcttattgc gccttttccc gggttacagc aattatcctt attctccgcc
    13081 attggtttaa cggcagccta ttttacagtg gtatgctggt atccaatatt ggcaagtaaa
    13141 ccaaatcaat ataacaaagt accgatgcaa cgagccttga gtcagtggct cttggtgtgg
    13201 caacaaccgc ttattcgatt agttttacca ttgagcttat tgattgctgc aagttttggt
    13261 gtctatatgg cgaaatatga tgatgatatt cgtcaacttc aagctttgcc acatgcactg
    13321 cagcagcaag agagagagat aaaagcgatt accggtgtca gtggctctca gcaactattg
    13381 cttgtacgag acagctctga agaagggtta ctgcaaaagt tagaaaccat atcgactaaa
    13441 ctggataaac gtaatgattt cggtggttat caatccataa gccgttatgt accgtcacaa
    13501 aaaagacaac aagaaaatta ccaattagtt aaacacctgt atcagcaaca atctgagtat
    13561 tatcaaagtg tcatacagca aaaaacagcg ctggattttt cacagcaata tcagcctctg
    13621 actatagatg gtttcttagc gtcaccaata tcagacacag tgggtttttt atggttaggc
    13681 aaaattgatg atgtatttgc ctcggtgatc gcgtttaacc aagaagtgaa cagtacggaa
    13741 ctgcaagcat ttgcagcgga taatcacatt acttatttaa ataaagcaga agagatttct
    13801 cgtttatttg cagattatcg acatcgtatt accgagctat tgttgtttgc ttatgccgct
    13861 attttcttac tgctgttacc tcgctataaa atgaaacaag cgtttttaat agtattgcca
    13921 ccatttatag caggttgcgc cggtttagct gtaaccgttt taactggcgt accgttgaat
    13981 ttgtttaact tactagcctt gatgttgatt ttagggatcg gaatagacta caccttgttt
    14041 tttgcagagc agaataagca aagtgatgat tcacataaag aaagcacgct attagcgata
    14101 tctttatctg cgttaacaac gattttgtca tttggtttat tggctttgag tgaaacccaa
    14161 gcgattcata gctttggtat tacggttctt acagggatta tcgtggcttg gttactggct
    14221 ccgttaagcc aacaaaataa agagagtaaa gggtatatta atacgctttc ggagaaaaaa
    14281 gatgcatcat aaggccataa aagtccgcca ccttatagcg cttgttctat cctttctttt
    14341 gatgggatgt gcagcaaaac cggtaaagca acccaaccaa gtagaagtat cacaaggtca
    14401 gtttattacc ttacctcagc ctgaagagta tggcagtgac ttatcactta gtcagttaat
    14461 ctcagttgaa tggcaaggaa aaaagaatca acttcctgtt caactgcaac tgacaggtaa
    14521 tgacttggta ttagctggct ttgcctcatg gggttctcgt ttattaagct tagattatga
    14581 tggtcaaaca ttagatactt acgtaatggc agggctaagt gacacgctac caccgccaga
    14641 gcaagtgcta tttaatgtca tgattacctt gtggccactt gaagcatggc aagaaagttt
    14701 aactagcatt ggctggcaac taaacgaaca tgacaatcat agaacattag tgactgaatc
    14761 aggaaaaaca atccttgatg ctcgttatga tgacatcaca gacaaattaa gtggtgaaat
    14821 tgtgttaacc agtccagatt ttggttatgt cataaaaatt aaaacgttct cgcattcaaa
    14881 tacaacaact caaaataata acggataaat taaatgagtg actttccttt atatatacat
    14941 gcgtgtggct cacattcagc catgggtaaa gaggttgatg aaatccatca agtattgagt
    15001 ggaaataaag cgccatcaat gcataaagag acaggttggc tcaataatgg aaaggcaaca
    15061 gtcgttggta aagcattagg tgagttacct ttagttcctg agcatttggc tcattgggat
    15121 accagaaata atcagcttgt tttgtccgct ttacagcaaa tcgaacctca aattaacgcc
    15181 gcaaaagaga aatacggttc agatcgtatt gctgttattg ttggtacgag tacttcaggg
    15241 atctcggatg gtgaagtcgc tttaaaagag aagatggagc aaggggcttt tcctgctggt
    15301 tatagctata aacaacaaga gttaggaaac cccgcggatt ttattgctca atatcttgat
    15361 atcaatggac caacgtatgc gatctcaacc gcttgttcct caagtggtcg tgttttctta
    15421 tcagcaaagc gtttattaaa agcagggttt gttgatgcgg ttatcgttgg cggtactgac
    15481 agtttatgcc gtttaacatt aaatggtttc aacagcctag aagcactatc aaatgagtat
    15541 tgtctgccgt ttagtcccaa tcgaaaaggg ataaacattg gtgaagctag cgcatatatg
    15601 ttgttaagtg cagagcaaaa agattctgat gatcctattg ctttattagg agctggagat
    15661 agctcagatg ctcaccatat atcggccccg caccctgaag gaaaaggggc aattgaagcg
    15721 atgaataaag cgctaacaga tgctggtttg gaacctaaag acattggtta tattaatgcg
    15781 catggaaccg ccacaccgct gaatgatgcc atggaatcta aagccgttca tgcggtgttt
    15841 ggtcattcag taccggtgag ctcaacaaaa ccattcactg ggcacacatt aggtgccgca
    15901 ggtatcattg aagcaagcat ttgttggcat attttaaagt ataatttacc gctacctaag
    15961 cagattaatg acggacagca agatgatgca ctggctgata ttcagcttgt ggtcgatcaa
    16021 ccactaagaa aaaaagccgt tattagtaat tcatttgcct ttggcggtaa taacatcagt
    16081 ttaatttttg gatgtatttg atgagtagta ttcctccttt aatcgacctt ttaccccatg
    16141 atacaccttt agctcttgtt gatgagttga ttgaggtaaa tgacttatcg gttcattgtc
    16201 aggtgacaat cggtgagaat aatgtctttt ttgatcatga gacgaaaacc gttcccgctt
    16261 gggttggtat tgagttcatg gcgcaaagta ttgctggttg gtctggctat catgcgtgga
    16321 aaaaaggcaa taagtcacca ataggctttt tacttggtag ccgtaaatac caagctgagt
    16381 gcagcgagtt tcaacaaggc gacacactcg acatttttgc agagcaagtg ttagagaata
    16441 atggcatggc cgtattcagt tgttatattg aacataaagg acaacagttg gcatcgagcc
    16501 aattaaacgt gttcgtgcca acagaagaaa aattagaaga gctcttaacg ccaaagcagt
    16561 aacgcagtag tagaggacaa catgaaaaga caagtattag tgacaggcgc cagtaaaggc
    16621 attggtcgcg caatttcaga gcaactagca aaggatggct tttatattgt tgttcattat
    16681 atgagtgata aagctggcgc agagacaacg ttagcagcaa ttcaagagca cggtggtgaa
    16741 gggcgcttaa ttcagttcga tattagtgac cgtgcacaat gtaaagcgac acttgagagt
    16801 gatatcgaaa ctcatggtgc atattatggt gtggtaagta atgctggcat tactcgtgat
    16861 accgctttcc ctgcaatgac agaagaagag tgggatggcg taattcatac caatttagac
    16921 agtttttata atgtacttca tccttgtgtt atgccgatgg ttcaaaagcg aaaaggcggt
    16981 cgaattgtga cgcttgcatc ggtgtcaggc atcatgggta accgtggtca aacgaactac
    17041 agcgcagcaa aagctggcgt tattggtgca actaaatctc ttgcgttaga gctagcaaaa
    17101 cgaaaaatca cagtaaactg tgttgcacct ggcttaattg atacaggcat ggttgatgag
    17161 catgtgaaag agcatgcatt gccacaagta ccacttcgaa gaatgggtga accagaagaa
    17221 gtagccggtt tagtaagtta cttaatgtca gatgttgcgg gttatgtgac tcgccaagtt
    17281 atttcaatta atggtggttt agtatgagtc gtcgtgtagt cgtaaccgga atgtctggtg
    17341 ttaccgcttt aggtagtgat tggaacaccg taggcgaaaa cctacgtaag tgtgaaaatg
    17401 ccactcaata tatgccaagc tttgaagtct acgatggtct taatactaag ctagcagccc
    17461 ctattaatga tttcacatta ccaaagcact atacccgtaa aaaaattcgt tctatgggac
    17521 gtgtttcttt gttatctacc gttgcttcag agcaagcctt agagcaaagt ggccttattg
    17581 gaaatgacat cttaaccaat ggccaaacag gtattgcctt tggttcctca accggttcta
    17641 ccgatgccgt tggtgctttt ggtgtgatgc ttaatgagaa atcaactcgt gctattacgg
    17701 caacaaccta tgtgcaaatg atgccacata ccgcagcggt taacgttggt ttattctttg
    17761 gtttacgtgg ccgagttatt ccaacaagca gcgcatgtac ttcaggcagc caagcgattg
    17821 gttatgctta tgaagcgatt aaacacggtt atcaaacggc aatggttgct ggtggcgcag
    17881 aagaattatg cccaacagaa tcagcagtat ttgatacatt attcgcaaca agtttaaaaa
    17941 acgatgcgcc aaaaacatcc ccaagcccat atgaccaaga tcgcgatggt cttgttattg
    18001 gtgaaggtgc tggtgcttta gttcttgaag agtatgaaca tgctaaagct cgtggcgcaa
    18061 cgatttacgc agagctagtt ggttttgcaa gtaactgtga tgctgcccat gtaacacaac
    18121 cacaaatgga aaccatgcaa atgtgtatgg aaatgtcgtt aagggatgcc aacttagcgc
    18181 cagagaaaat tggttatgtt tcagcgcatg gtaccgcaac agaacgtggt gatatagcag
    18241 aaagtacagc aacagagaac gtatttggct catcaatgcc aatcagttca ttgaagtctt
    18301 attttggtca tacattaggt gcatgtggtg cgattgaagc gtggttatcc attgagatga
    18361 tgcgtaacaa ttggtttagc ccaacattga acttaaataa tgtagatgag cgttgcggta
    18421 agttagatta cattacaggt acaggtcgtg aaattgagtg tgattacatc atgagtaaca
    18481 actttgcttt tggtggtatt aatacatcac ttatctttaa gcgctggact gaagagtag
//
