LOCUS       BGC0000841             11023 bp    DNA     linear   CON 25-JUL-2016
DEFINITION  Streptomyces clavuligerus ATCC 27064 plasmid pSCL4, whole genome
            shotgun sequence.
ACCESSION   BGC0000841
VERSION     BGC0000841
KEYWORDS    .
SOURCE      Streptomyces clavuligerus
  ORGANISM  Streptomyces clavuligerus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
COMMENT     This record represents the linear plasmid.
            ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 14:18:15
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..1644
                     /EC_number="2.5.1.66"
                     /codon_start=1
                     /gene="ceaS1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TPP_enzyme_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TPP_enzyme_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1055:pyruvate oxidase/decarboxylase (Score: 488.8;
                     E-value: 3.6e-148)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="SCLAV_p1074"
                     /note="COG:COG0028 Thiamine pyrophosphate-requiring enzymes
                     [acetolactate synthase, pyruvate dehydrogenase
                     [cytochrome], glyoxylate carboligase, phosphonopyruvate
                     decarboxylase]."
                     /product="Carboxyethyl arginine synthase isoenzyme 1"
                     /protein_id="EFG04560.1"
                     /sec_met_domain="TPP_enzyme_N (E-value: 3.6e-42, bitscore:
                     132.6, seeds: 75, tool: rule-based-clusters)"
                     /sec_met_domain="TPP_enzyme_C (E-value: 2.2e-34, bitscore:
                     107.2, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLERLHQYGVDHVFGVVGREASAILFDEVEGLDFVLTRHEFTAGV
                     MADVLARITNRPQACFATLGPGMTNLATGVATSALDRSSVIALAAQSESYDCYPNVTHQ
                     CLDSTAVMGPLTKFSVQLERGEDIVNLVDSAVLNSRIEPVGPSFISLPVDLLGAELNGT
                     PTDAPLVRATATHALDADWRARLDEAAELVREAENPLLVVGSAVIRAGAVDALRALAER
                     LNIPVVTTYTAKGVLPHDHPLNYGAISGYMDGILGHPALDEIFGPADLLLAIGYDYAED
                     LRPSMWTRGRAKTTVRVAPEVNPIPELFRADIDIVTNVAEFVTALDDATSGLAPKTRHD
                     LSALRARVAEFLADPTEYEDGMRVHQVIDCMNSVLDNGTFVSDIGFFRHYGVLFAKSDQ
                     PYGFLTSAGCSSFGYGLPAAMAAQIARPGEPVFLIAGDGGFHSNSADIETAVRLGLPIV
                     MVVVNNDRNGLIELYQNLGHQRSHAPAVGFGSVDFVQLAEANGCEAVRATDRTSLLAAL
                     TKGAGLGRPFLIEVPVAYDFQSGGFAALAI"
     protocluster    1..11023
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[1667:3251]"
                     /cutoff="20000"
                     /detection_rule="(BLS or CAS or tabtoxin)"
                     /neighbourhood="10000"
                     /product="blactam"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1668..3251
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(BLS or CAS or tabtoxin)"
                     /neighbourhood="10000"
                     /product="blactam"
                     /protocluster_number="1"
     cand_cluster    1..11023
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(BLS or CAS or tabtoxin)"
                     /kind="single"
                     /product="blactam"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..11023
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000841"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..11023
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="blactam"
                     /region_number="1"
                     /rules="(BLS or CAS or tabtoxin)"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            1..1644
                     /gene="ceaS1"
                     /locus_tag="SCLAV_p1074"
     gene            1668..3251
                     /gene="bls1"
                     /locus_tag="SCLAV_p1075"
     CDS             1668..3251
                     /codon_start=1
                     /gene="bls1"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     blactam: BLS"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) GATase_7"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Asn_synthase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1177:asparagine synthase (glutamine-hydrolyzing)
                     (Score: 342; E-value: 1.2e-103)"
                     /gene_kind="biosynthetic"
                     /locus_tag="SCLAV_p1075"
                     /note="COG:COG1620 L-lactate permease."
                     /product="Beta-lactam synthetase isoenzyme 1"
                     /protein_id="EFG04561.1"
                     /sec_met_domain="GATase_7 (E-value: 1.8e-17, bitscore:
                     52.2, seeds: 307, tool: rule-based-clusters)"
                     /sec_met_domain="BLS (E-value: 1.3e-168, bitscore: 549.7,
                     seeds: 11, tool: rule-based-clusters)"
                     /sec_met_domain="Asn_synthase (E-value: 9.8e-24, bitscore:
                     73.2, seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPGPDLVYGFRVRIGTEGRPGGGPGGHSEPGSAPRFAVRGTHVPV
                     HDGTAYPLWSGTAVTLGRPPVLVADGQVRLLLAGELYNRAELTGALGGSSAALGDAELL
                     LAAWRRWGPGAFRLLNGRFAALLTDASTGATVAATDHAGSVPLWLRADVTGLSAATEAK
                     TLAHEPGRPLGLSGTHTAPGAAGVCRVPAGTALLLHGVGGSDITARAVRTWTPPLSRAL
                     PGEREAVDLVGERLATAVRTRLRGGEAAPTVVLSGGIDSGGVAAHTAALAPGTRSVSMG
                     TEVSDEFDAARSVAVHLGTAHSEIRLHSAELVRELPWAVAAAEITDPTVLEYLLPLVAL
                     YRRLDTGPLRILTGYGADIPLGGMHRRTASLWSLDDEIAGDMAGFDGLNEMSPVLAGIA
                     GKWTTHPYWDRAVLDALVSLEPGLKRRRGTDKWVLRQALSGLLPAETVARPKLGIHEGS
                     GTTSAWTGLLLAEGIRRDEVTAVKGAMARRLYDAVVIDTVPPEDVDFGETVRRSVDAVR
                     RLRLQGRVVV"
     gene            3248..4621
                     /gene="pah1"
                     /locus_tag="SCLAV_p1076"
     CDS             3248..4621
                     /codon_start=1
                     /gene="pah1"
                     /locus_tag="SCLAV_p1076"
                     /note="COG:COG0010 Arginase/agmatinase/formimionoglutamate
                     hydrolase, arginase family."
                     /product="Proclavaminate amidinohydrolase"
                     /protein_id="EFG04562.1"
                     /transl_table=11
                     /translation="MSGQNGTGGQNGTGGGNGTGGGADGAAGVGAGTGPQSGNGRRNGR
                     SGLGGRGSRGPDGAAGAGTGFHAGTGARSGTGVGTGTGARTGWPGVGTGTGAGPGARGT
                     ARVSTAVSPRYAQPATFMRLRHRPDPIGHDVVVVGAPYDGGTSYRPGARFAPRAIRHES
                     SLIHGVGIDRGPGVFDRIDVVDGGDIDLSPFSMDLAMDTATVALTRLLERNDAFLMLGG
                     DHSLSLAALRAVHARHGRVAVLHLDAHSDTNPPVYGGTYHHGTPFRWAIEEGLVDPERL
                     VQVGIRGHNPRPDSLDYARGHGVSIVTAADFTRRSPRGIAEQIRRTVGGLPLYVSVDID
                     VVDPAYAPGTGTPAPGGLSSREVLTLLDVVGQLRPVGFDVVEVSPAYDPSGITSLLAAE
                     IGAELLYQYARATTSPASAPVDSPLPPGAAADDAENAENAVDAVDAESAVDFAGQRWG"
     gene            complement(4813..6171)
                     /gene="oat1"
                     /locus_tag="SCLAV_p1077"
     CDS             complement(4813..6171)
                     /EC_number="2.3.1.35"
                     /codon_start=1
                     /gene="oat1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1220:bifunctional ornithine (Score: 420.2; E-value:
                     1e-127)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="SCLAV_p1077"
                     /note="COG function: Amino acid transport and metabolism.
                     KEGG: Arginine and proline metabolism Metabolic pathways.
                     COG: COG1364 N-acetylglutamate synthase [N-acetylornithine
                     aminotransferase]."
                     /product="Probable glutamate N-acetyltransferase 1"
                     /protein_id="EFG04563.1"
                     /transl_table=11
                     /translation="MPRRRSRGPCPCPCSGAPPSAVAPFPFQRAVEPPPSAPCHGGRPP
                     PDGARGARQAEGKSRPMRASSPRGFRVHHGHAGIRGSHADLAVIASDVPAAVGAVFTRS
                     RFAAPSVLLSRDAVADGIARGVVVLSGNANAGTGPRGYEDAAEVRHLVAGIVDCDERDV
                     LIASTGPVGERYPMSRVRAHLRAVRGPLPGADFDGAAAAVLGTAGARPTIRRARCGDAT
                     LIGVAKGPGTGPAEQDDRSTLAFFCTDAQVSPVVLDDIFRRVADRAFHGLGFGADASTG
                     DTAAVLANGLAGRVDLVAFEQVLGALALDLVRDVVRDSGCGGALVTVRVTGAHDTEQAG
                     RVGRAVVDAPSLRAAVHGPAPDWAPVAAVAGGHGDEGPGRSPGRITIRVGGREVFPAPR
                     DRARPDAVTAYPHGGEVTVHIDLGVPGRAPGAFTVHGCDLLAGYPRLGAGRAV"
     misc_feature    complement(5599..5601)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(6622..8145)
                     /gene="cvm6P"
                     /locus_tag="SCLAV_p1078"
     CDS             complement(6622..8145)
                     /NRPS_PKS="Domain: Aminotran_3 (86-432). E-value: 5.4e-92.
                     Score: 300.2. Matches aSDomain:
                     nrpspksdomains_SCLAV_p1078_Aminotran_3.1"
                     /codon_start=1
                     /gene="cvm6P"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013:aminotransferase class-III (Score: 404.3;
                     E-value: 9.3e-123)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="SCLAV_p1078"
                     /note="COG function: Coenzyme transport and metabolism.
                     COG: COG0161 Adenosylmethionine-8-amino-7-oxononanoate
                     aminotransferase."
                     /product="Putative pyridoxal phosphate-dependent
                     aminotransferase"
                     /protein_id="EFG04564.1"
                     /sec_met_domain="Aminotran_3 (E-value: 1.2e-91, bitscore:
                     296.0, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAGRRPPRPTEEPPLDTYAADTYPRSGTHPEPRPDAPPHARPGTR
                     PGTRSEPRPDPGAEAAWLLAADRAHMFHPVLPRGREDRTVLVSGRGCTVRDTEGRTYLD
                     ASSVLGLTQIGHGREEIAQAAAEQMRTLGHFHTWGTISNDKAIRLAARLTDLAPQGLQR
                     VYFTSGGGEGVEIALRMARYFHHRTGSPERTWILSRRTAYHGIGYGSGTVSGSPAYQDG
                     FGPVLPHVHHLTPPDPYHAELYDGEDVTEYCLRELARTIDEIGPGRIAAMIGEPVMGAG
                     GAVVPPPDYWPRVAALLRSHGILLILDEVVTAFGRTGTWFAAEHFGVTPDLLVTAKGIT
                     SGYVPHGAVLLTEEVADAVNGETGFPIGFTYTGHPTACAVALANLDIIEREGLLENAVK
                     VGDHLAGRLAALRGLPAVGDVRQLGMMLAVELVSDKTARTPLPGGTLGVVDALREDAGV
                     IVRATPRSLVLNPALVMDRATADEVADGLDSVLRRLAPDGRIGAAPRRG"
     aSDomain        complement(6850..7887)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_SCLAV_p1078_Aminotran_3.1"
                     /evalue="5.40E-92"
                     /label="SCLAV_p1078_Aminotran_3.1"
                     /locus_tag="SCLAV_p1078"
                     /protein_end="432"
                     /protein_start="86"
                     /score="300.2"
                     /tool="antismash"
                     /translation="VSGRGCTVRDTEGRTYLDASSVLGLTQIGHGREEIAQAAAEQMRT
                     LGHFHTWGTISNDKAIRLAARLTDLAPQGLQRVYFTSGGGEGVEIALRMARYFHHRTGS
                     PERTWILSRRTAYHGIGYGSGTVSGSPAYQDGFGPVLPHVHHLTPPDPYHAELYDGEDV
                     TEYCLRELARTIDEIGPGRIAAMIGEPVMGAGGAVVPPPDYWPRVAALLRSHGILLILD
                     EVVTAFGRTGTWFAAEHFGVTPDLLVTAKGITSGYVPHGAVLLTEEVADAVNGETGFPI
                     GFTYTGHPTACAVALANLDIIEREGLLENAVKVGDHLAGRLAALRGLPAVGDVRQLGMM
                     LAVELV"
     gene            complement(8276..11023)
                     /gene="cvm7P"
                     /locus_tag="SCLAV_p1079"
     CDS             complement(8276..11023)
                     /codon_start=1
                     /gene="cvm7P"
                     /gene_functions="regulatory (smcogs)
                     SMCOG1041:transcriptional regulator, SARP family (Score:
                     290.4; E-value: 5.8e-88)"
                     /gene_kind="regulatory"
                     /locus_tag="SCLAV_p1079"
                     /note="COG function: Signal transduction mechanisms. COG:
                     COG3629 DNA-binding transcriptional activator of the SARP
                     family."
                     /product="Putative transcriptional regulator"
                     /protein_id="EFG04565.1"
                     /transl_table=11
                     /translation="MVGRDVGRWRRCGSRSFPVLLGSASRRVGPLPRVGRDDAAGSPRG
                     RDPRTGEGAAVSDVFASEKSSPGVRTRAATSPPRSVISAVGAVSHAEKIGYAVYECSDE
                     VRHDVPGLPGPSPSITVLGCLGVRADGRKLELGPPRQRAVFALLLINAGSVVPVDSIVF
                     RIWGNSPPGAVTATLQSYVSRLRKLLAECVLPDGSTPELLHQPPGYTLALGTEHIDANR
                     FEQAIRTGRRLSREEQHQEARAVLCQALLSWGGTPYEELSAYDFAVQEANRLEQLRLGA
                     VETWAHCCLRLGRDEEVMDQLKPEVQRNPLRERLIGQLMQAQYRLGCQADALRTYEATR
                     RALAEELGTDPGKELAALHAAILRQDNGLDRVVPASAPPSAGVGRGAVTVSVPAQRSRP
                     LTRPVAGRARVPGAMTVAAGAGAAPASASGSVSASVSGSGSGSGSAPASVPTFFPGSVS
                     GSASVAASVAAPVSGHVSGPGSAFGSVALHRPQTLRGEPVHGGAQGMRTGQVFPTLPPF
                     VGRGDELRGLLESATSAFHTSGRVAFVVGEAGSGKTRLLSELERSVPDSVRTVWASCSE
                     SEDRPDYWPWTTVLRHLYAMWPERMHGFPGWLRRALAELLPEVGPEPQGPHSPDGGEEN
                     SGNGDGAGDGDSTPAHTLTLAPALAPPRSREARFTLHDAVCQALLRTVREPVVIMLEDM
                     ERADAPSLALLRLLVEQLRTVPLLLVVTTRTFRLAHDAELRRAAAVILQSTGARRVLLN
                     ALDARATGELAGGMLGKAPDTLLVRALHERSAGNPYFLVQLLRSLRQGLAAAWETEIPD
                     ELAGVVLQRLSSVPPAVRRVLDICAVVERSCERRVIETVLRHEGIPLENVRTAVRGGLL
                     EEDPDDPGRLRFVHPLVREAVWDDLENTRRPVSRSSALGALATV"
ORIGIN
        1 atgctggaac gtcttcacca gtacggtgtc gaccatgtat tcggcgtcgt cggccgggag
       61 gcgtccgcca ttctcttcga cgaggtcgaa ggactcgact tcgtcctgac ccggcacgag
      121 ttcaccgccg gggtgatggc ggacgtcctc gcccggatca ccaaccgccc ccaggcgtgc
      181 ttcgcgaccc tgggccccgg catgaccaac ctggccaccg gcgtcgccac ctccgccctg
      241 gaccgcagct cggtcatcgc gctggccgcg cagtccgagt cgtacgactg ctaccccaac
      301 gtcacccacc agtgcctgga cagcaccgcc gtgatgggcc cgctgaccaa gttcagcgtc
      361 cagctcgaac gcggcgagga catcgtcaac ctcgtcgaca gcgccgtcct caacagccgg
      421 atcgagcccg tgggtcccag cttcatcagc ctgccggtcg acctcctcgg cgccgagctg
      481 aacggcaccc ccaccgacgc ccccctggtc cgggccaccg ccacccacgc cctggacgcc
      541 gactggcgcg cccgcctcga cgaggccgct gagctggtgc gcgaggccga gaaccccctc
      601 ctcgtcgtcg gtagcgccgt catccgcgcc ggggccgtcg acgccctgcg cgccctcgcc
      661 gagcggctga acatccccgt cgtcaccacc tacaccgcca agggcgtcct gccgcacgac
      721 cacccgctca actacggcgc catcagcggc tacatggacg gcattctcgg ccacccggcc
      781 ctcgacgaga tcttcggccc cgccgacctc ctcctggcga tcggctacga ctacgccgag
      841 gacctgcgcc cctccatgtg gacgcggggc cgggccaaga ccacggtccg ggtcgccccc
      901 gaggtcaacc cgatcccgga gctgttccgc gccgacatcg acatcgtcac caacgtcgcc
      961 gaattcgtca ccgcgctcga cgacgcgacc tcgggcctcg cccccaagac ccggcacgac
     1021 ctcagcgccc tgcgcgcccg cgtcgccgaa ttcctcgccg accccaccga gtacgaggac
     1081 ggcatgcggg tccaccaggt gatcgactgc atgaactccg tcctcgacaa cggcaccttc
     1141 gtcagcgaca tcggcttctt ccgccactac ggcgtgctct tcgccaagtc cgaccagccg
     1201 tacggattcc tcacctccgc gggctgctcc agcttcggct acggactgcc cgccgccatg
     1261 gccgcccaga tcgcccggcc cggcgagccc gtcttcctca tcgcgggcga cggcggcttc
     1321 cactccaaca gcgccgacat cgagacggcc gtgcgcctgg gcctgccgat cgtcatggtc
     1381 gtcgtcaaca acgaccgcaa cggcctgatc gagctgtacc agaacctcgg acaccagcgc
     1441 tcccacgccc ccgccgtcgg cttcggaagc gtcgacttcg tccagctcgc cgaggccaac
     1501 ggctgcgagg ccgtccgcgc caccgaccgc acctcgctgc tcgccgccct caccaagggc
     1561 gccggactcg gccgcccgtt cctgatcgag gtaccggtgg cctacgactt ccagtccggc
     1621 ggtttcgccg ccctggccat ctgaccaccg tggaggacga gttcaccatg cccggccccg
     1681 acctcgtgta cggattccgg gtgcgcatcg gcaccgaggg ccgccccggc ggcggccccg
     1741 gcggtcactc cgaacccggc agcgcacccc gcttcgccgt ccgcgggacc catgtccccg
     1801 tgcacgacgg caccgcgtac ccgctctgga gcggaacggc cgtgaccctg ggccgtccgc
     1861 ccgtcctggt cgccgacggc caggtccggc tgctcctggc gggcgagctg tacaaccgcg
     1921 ccgagctgac cggagcgctc ggcggctcct ctgccgccct cggcgacgcc gaactgctgc
     1981 tggccgcctg gcggcgctgg ggccccgggg ccttccggct cctgaacgga cggttcgccg
     2041 cactgctcac cgacgcctcc accggcgcga ccgtcgcggc caccgaccac gccggttcgg
     2101 taccgctgtg gctgcgcgcc gacgtgacgg ggctgagcgc cgccaccgag gcgaagaccc
     2161 tggcgcacga gccgggccgg ccgctgggcc tgtccggcac ccacaccgcg ccgggggcgg
     2221 cgggcgtctg ccgggtcccc gccgggaccg ccctcctgct gcacggagtc ggcggctccg
     2281 acatcaccgc cagggcggtc cgcacctgga cacccccgct ctcccgggcg ctgcccggcg
     2341 aacgggaggc ggtggacctg gtcggcgaac gcctcgccac ggcggtccgc acccggctgc
     2401 gcggcgggga ggcggccccc accgtcgtcc tgtccggcgg catcgactcc gggggagtcg
     2461 ccgcccacac ggcggccctg gcacccggga cacggtccgt gtcgatgggc accgaggtgt
     2521 ccgacgagtt cgacgcggcc cgctcggtcg ccgtccacct gggcaccgcg cacagcgaga
     2581 tccggctcca ctcggccgaa ctcgtcaggg aactgccctg ggcggtcgcc gccgcggaga
     2641 tcaccgaccc cacggtcctg gagtacctgc tgccgctcgt cgccctctac cggcggctcg
     2701 acaccgggcc gctccgcatc ctcaccgggt acggcgccga catcccgctc ggcggtatgc
     2761 accggcgcac ggcctcgctc tggtccctcg acgacgagat cgcgggcgac atggcgggct
     2821 tcgacggcct caacgagatg tcccccgtcc tcgcgggcat cgccgggaag tggaccaccc
     2881 acccgtactg ggaccgcgcg gtcctggacg cgctggtctc cctcgaaccc gggctcaaac
     2941 gccggcgggg caccgacaag tgggtgttgc ggcaggccct ctccggcctg ctgcccgccg
     3001 agaccgtggc ccgccccaag ctgggcatcc acgaggggtc cggcaccacc agcgcgtgga
     3061 ccggactgct cctcgccgaa gggatccggc gcgacgaggt gacggccgtc aagggcgcca
     3121 tggcacggcg cctgtacgac gcggtggtca tcgacacggt gccgccggag gacgtggact
     3181 tcggcgagac ggtgcggcgc tccgtcgacg cggtgcgcag gctcaggctc cagggccggg
     3241 tggtcgtatg agcggccaga acggcaccgg tggccagaac ggcaccggcg gcggcaacgg
     3301 caccggcggc ggcgcggacg gtgcagcggg cgtgggcgcg ggcaccggcc cgcagagcgg
     3361 caacggccgc aggaacggca ggagcggtct cggagggagg ggcagccgcg gcccggacgg
     3421 cgccgcgggc gcgggaacgg gattccacgc gggaacggga gcccgttcgg gaacgggggt
     3481 cggcacggga acgggagccc gcacggggtg gccgggcgtc ggtacgggaa cgggagcagg
     3541 gccgggtgcg cggggaacgg ccagggtgtc caccgccgtc tccccgcgct acgcccaacc
     3601 ggcgaccttc atgcggctgc gccaccggcc cgacccgatc ggccatgacg tggtggtcgt
     3661 cggcgccccg tacgacggag gcaccagcta ccggcccggc gcgcggttcg cgccgcgcgc
     3721 catccggcac gagtccagcc tgatccacgg cgtcggcatc gaccggggcc caggggtctt
     3781 cgaccggatc gacgtggtcg acgggggcga catcgacctc agccccttct cgatggacct
     3841 ggcgatggac accgcgacgg tcgccctgac ccggctcctg gaacgcaacg acgcgttcct
     3901 gatgctgggc ggggaccact cgctctccct ggccgccctg cgcgccgtgc acgcccgcca
     3961 cggccgggtc gccgtcctgc acctggacgc gcacagcgac accaacccac ccgtctacgg
     4021 cggcacctac caccacggca cccccttccg ctgggccatc gaagagggcc tggtggaccc
     4081 ggagcgcctg gtccaggtcg gcatccgcgg ccacaatccg cggcccgact ccctggacta
     4141 cgcgcgcggg cacggcgtca gcatcgtcac cgccgccgac ttcacccggc gctcaccgcg
     4201 cggcatcgcc gagcagatcc ggcgcaccgt cggcggcctg ccgctgtacg tctccgtcga
     4261 catcgacgtc gtcgacccgg cgtacgcccc gggcaccggc acaccggccc ccggcgggct
     4321 gtcctcgcgc gaggtgctga ccctgctcga cgtggtcggg cagctcaggc ccgtcggctt
     4381 cgacgtggtc gaggtgtccc cggcgtacga cccgtcgggg atcacctccc tgctggcggc
     4441 ggagatcggg gccgaactgc tctaccagta cgcccgcgcc accacgtcgc ccgcgtcggc
     4501 accggtggac tctcccctgc caccgggggc ggcggcggac gacgccgaga acgccgagaa
     4561 cgcggtggac gcggtggacg ccgagagcgc cgtggacttc gccgggcagc ggtgggggta
     4621 gggcggccgg ggacagcgga cggaacgacg ggagccgggc gcnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnttcctgcg ctcccgcgcc ctcgcggtcg ccgtccgccc
     4801 gggagcgccc gttcagacgg cccggccggc gccgaggcgc gggtaccccg ccaggaggtc
     4861 gcagccgtgg accgtgaacg cgccgggcgc ccggcccggg acaccgaggt cgatatggac
     4921 ggtcacctcg ccgccgtgcg gatacgcggt gacggcgtcc gggcgggccc ggtcgcgggg
     4981 ggcggggaag acctcccggc cgccgacccg gatcgtgatc cgcccgggag accggccggg
     5041 gccttcgtcc ccgtgtccac ccgccacggc ggcgaccggc gcccagtcgg gtgccgggcc
     5101 gtgcaccgcg gccctcagcg acggcgcgtc gaccaccgcc cggcccacgc gcccggcctg
     5161 ctcggtgtcg tgggccccgg tgacccgcac cgtgaccagg gcgccgccgc agccgctgtc
     5221 ccggacgacg tccctgacca ggtccagcgc cagcgcgccc aggacctgtt cgaacgcgac
     5281 gaggtccacc cggcccgcga gcccgttggc gagaacggcc gccgtgtcgc cggtggaggc
     5341 gtcggcgccg aagcccagcc cgtggaaggc gcggtccgcg acccggcgga agatgtcgtc
     5401 gaggacgacg gggctcacct gggcgtccgt gcagaagaac gccagcgtcg accggtcgtc
     5461 ctgctccgcc gggcccgtac ccgggccctt ggcgacaccg atcagcgtcg cgtcgccgca
     5521 ccgcgcccgc cggatcgtgg gacgggcgcc cgcggtgccc agcacggccg ccgccgcgcc
     5581 gtcgaagtcg gcacccggta agggcccgcg caccgcccgc agatgggccc ggacacggga
     5641 catcggatac cgctcgccga cgggtcccgt ggaggcgatc agcacatccc tctcgtcgca
     5701 gtcgacgatc ccggccacca gatggcgcac ctccgcggcg tcctcgtacc cccgcgggcc
     5761 cgtcccggcg ttggcgttgc cggacagcac caccacgccc cgggcgatcc cgtcggcgac
     5821 cgcgtcccgg ctgagcagca cactcggcgc ggcgaaccgc gaacgggtga acaccgcgcc
     5881 gaccgccgcg ggaacgtcgg aggcgatgac ggcgaggtcc gcgtgggacc ccctgatccc
     5941 ggcgtgaccg tggtgcacgc ggaaccctct gggcgaagag gcacgcatcg gacgggactt
     6001 cccttcggct tgccgggctc cgcgcgcccc gtcgggcggc ggtctcccac cgtggcacgg
     6061 ggcgcttggc ggcggctcga cagcgcgctg gaacgggaac ggcgccacag cggagggagg
     6121 agcgcccgag caggggcagg ggcaggggcc acgggaacgg cgccggggca cggaaaaggc
     6181 gccgggaaac ggggaaggcg ccgggacnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnnnnagc ggatacgtct gaccgatctg gagcgccgac cggagaacgt ccgctccacg
     6361 gcgaatctgt tcgggctgcc gctcttctgc cattacggtg tcggccgtat cggcggcccg
     6421 gggttcgggg cctggcggga gctggccgcg cacaccatga cgacggactg ggtgatcgcc
     6481 ggacaccacg agggcttccc gctgatgcac cactggcggg tgctgcccgc cccggaccgg
     6541 ccgcttccgg aggaactcgc cgatgtggaa cgggccgtcg cccactgggg agccccccgc
     6601 gggtggcggc ccgcggtctc gtcacccccg ccggggggcc gcgccgatcc gcccgtcggg
     6661 tgccagccgc cgcagcaccg agtccagccc gtccgccacc tcgtccgccg tggcccggtc
     6721 catcacgagc gccggattga ggaccaggga gcgcggcgtg gcccggacga tgacgcccgc
     6781 gtcctcgcgc agcgcgtcca cgaccccgag ggtgccgccc ggcagcgggg tgcgggccgt
     6841 cttgtccgac accagctcga cggcgagcat catgcccagt tgccggacgt cccccacggc
     6901 gggcagcccg cgcagggccg ccagccgccc ggcgaggtgg tcgcccacct tcaccgcgtt
     6961 ctccagcagc ccttcccgtt cgatgatgtc gagattggcg agcgcgacgg cgcacgccgt
     7021 ggggtgaccg gtataggtga agccgatcgg gaaccccgtc tccccgttca cggcgtccgc
     7081 gacctcctcg gtcaggagca ccgccccgtg cgggacatac ccggaggtga tgcccttcgc
     7141 ggtcaccagc agatcggggg tcaccccgaa gtgctcggcc gcgaaccagg tccccgtgcg
     7201 gccgaacgcg gtgacgacct cgtccaggat cagcaggatg ccgtgggagc gcagcagcgc
     7261 ggcgacgcgc ggccagtagt ccggcggcgg gacgacggcg ccgcccgcgc ccatgaccgg
     7321 ctccccgatc atcgcggcga tccgcccggg gccgatctcg tcgatggtgc gggcgagttc
     7381 gcgcaggcag tactccgtga cgtcctcgcc gtcgtacagc tcggcgtggt acgggtcggg
     7441 cggcgtgagg tggtgcacat ggggcagcac cgggccgaac ccgtcctggt aggcgggcga
     7501 gcccgacacc gtaccgctgc cgtagccgat gccgtggtag gcggtgcggc gcgacaagat
     7561 ccaggtgcgc tccgggctgc cggtgcggtg gtggaagtaa cgggccatgc gcagggcgat
     7621 ctcgacgccc tcgccgccgc cgctggtgaa gtagacgcgc tggagaccct ggggcgccag
     7681 gtcggtgagg cgcgcggcca gtcggatggc cttgtcgttg ctgatggtgc cccaggtgtg
     7741 gaagtgaccg agtgtccgca tctgctcggc ggcggcctgc gcgatctcct cacgtccatg
     7801 gccgatctgg gtcagtccga gcaccgacga ggcgtcgaga taggtgcgcc cttcggtgtc
     7861 ccgtacggtg cagccgcggc cggagaccag aacggtgcgg tcctcgcggc cccggggcag
     7921 gaccgggtgg aacatatggg cgcggtccgc cgcgagcagc cacgcggcct cggcgcccgg
     7981 gtccgggcgc ggctcggaac gggtgccggg acgggtcccg ggacgcgcgt ggggaggtgc
     8041 gtcgggacgc ggctcggggt gggtgccgga ccgcgggtac gtatccgctg cgtacgtgtc
     8101 caatgggggc tcctcggtgg gccgtggggg gcggcgaccg gccacggccg ccgcccgccc
     8161 agtgtcggca ccgccccttg cacccggctg cgcgtccggc tgccccgggg gatcaagccc
     8221 ggcgtcgggg agcgcgcaag cgccgcccgc cgccgaggac cccggggccc gggactcaga
     8281 ccgtggccag cgccccgagc gcggaggaac gggacacggg ccgacgggtg ttctccaggt
     8341 cgtcccagac ggcctcccgg accagcggat gcacgaacct cagccgcccg gggtcgtcgg
     8401 ggtcttcctc cagcagaccg ccgcggaccg ccgtacggac gttctccagc gggattccct
     8461 catggcgcag cacggtctcg atcacacgcc gttcgcaact gcgctccacg accgcgcaga
     8521 tgtcgagcac ccggcgcacg gcgggcggca cgctcgacag ccgttgcagc acgaccccgg
     8581 ccagctcgtc cgggatctcc gtctcccagg cggcggcgag cccctgccgg agcgagcgga
     8641 ggagctggac gaggaagtac gggttcccgg cggagcgctc gtgcagggcc cgtacgagga
     8701 gggtgtccgg ggccttgccc agcatccctc cggcgagttc cccggtggcc cgtgcgtcca
     8761 gggcgttcag caggacccgg cgcgcgccgg tcgactggag gatcacggcg gcggcccgtc
     8821 gcagctcggc gtcgtgcgcg agccggaagg tgcgcgtggt gaccacgagc agcaggggga
     8881 cggtgcgcag ttgctccacc aggaggcgca gcagggcgag cgagggggcg tcggcccgct
     8941 ccatgtcctc cagcatgatc accacgggtt cgcggaccgt gcgcagaagc gcctggcaca
     9001 cggcgtcgtg cagggtgaaa cgagcctctc tggagcgcgg gggcgcgaga gcgggcgcga
     9061 gcgtgagggt gtgcgccggg gtgctgtccc cgtcgcccgc accgtccccg ttgccgctgt
     9121 tctcctcgcc cccgtcgggg gagtgcggcc cctgtggctc cgggcccacc tcgggaagca
     9181 gttccgcgag tgcgcgccgc agccaaccgg ggaatccgtg catacgttcc ggccacatcg
     9241 cgtacagatg ccgcagcacg gtcgtccacg gccagtagtc gggccggtcc tcactctccg
     9301 aacaggacgc ccagacggtg cgcacactgt ccggaaccga gcgctccaac tcggagagga
     9361 gccgggtctt gccgctgccc gcctcgccga cgacgaacgc cacccgcccc gaggtgtgga
     9421 acgcggacgt cgcggactcc agcagaccgc gcagctcgtc gccgcgcccg acgaacggcg
     9481 gcagcgtggg gaacacctgc ccggtgcgca tcccctgcgc gcccccgtgg accggctcgc
     9541 cccggagggt ctgcggccgg tggagcgcca cggacccgaa agcggacccg ggcccggaga
     9601 catggccgga aacgggcgcg gctacggacg cggcaacgga cgccgagcca gaaacggagc
     9661 cgggaaagaa ggtgggaacc gacgcaggag cggagccgga gccggagccg gagccggaaa
     9721 cggacgcgga aacggagccg gaggcggacg cgggggccgc ccccgcgccc gccgccaccg
     9781 tcatcgcccc cgggacccgc gcccgccccg ccaccggccg cgtcaacggc ctcgaccgct
     9841 gtgccgggac cgacaccgtc acggcccccc gcccgacccc cgccgacggc ggcgcggacg
     9901 ccgggacgac gcggtccaga ccgttgtcct gacgcaggat cgccgcgtgc agcgccgcca
     9961 gctccttgcc cggatcggtc cccagctcct cggccagggc ccgccgcgtc gcctcgtacg
    10021 tcctgagcgc gtccgcctgg caccccagcc ggtactgcgc ctgcatgagc tgcccgatca
    10081 gccgctcccg cagcggattg cgctgcacct ccggcttgag ctggtccatc acctcctcgt
    10141 cccgccccag ccgcagacag cagtgcgccc atgtctccac ggcgcccagc cggagctgct
    10201 ccagccgatt ggcctcctgg acggcgaagt cgtacgcgct cagctcctcg tacggtgtcc
    10261 cgccccagct cagcagggcc tggcagagca cggcccgcgc ctcctggtgc tgctcctcgc
    10321 gcgagagccg gcgccctgtc ctgatggcct gctcaaaacg gttcgcgtcg atgtgctcgg
    10381 tgccgagcgc gagggtgtag cccggcggct ggtgcagcag ttcgggtgtc gaaccgtccg
    10441 ggagcacaca ctcggccagg agtttccgca gccgggacac ataggactgg agcgtcgcgg
    10501 tgaccgcgcc cggtggtgag ttgccccaga tacggaagac gatcgagtcg accggcacca
    10561 cactgcccgc gttgatgagc agcagggcga aaacggcccg ctgacgcgga gggcccagct
    10621 ccagtttccg gccgtcggcg cgtacgccca gacagcccag gacggtgatg gacggtgacg
    10681 gacccggcag gccggggacg tcgtgacgaa cctcatcgct gcactcgtac actgcgtatc
    10741 cgatcttctc tgcgtggctg acggcgccga cggcgctgat gacagagcgc ggtggggacg
    10801 ttgccgcgcg ggtccggaca ccgggcgaac tcttctcgga tgcgaagacg tccgacacgg
    10861 cagctccttc gccggttcgc ggatctctcc cccgtggaga acccgcagcg tcgtcgcggc
    10921 ccactcgggg aagcggaccg actctccgcg acgcacttcc cagaagtact gggaacgatc
    10981 ggcttccgca tcgccgccag cggccaacat cccttcccac caa
//
