BGC0000845

BGC0000845: clavulanic acid biosynthetic gene cluster from Streptomyces clavuligerus ATCC 27064

View antiSMASH-generated output

Download Cluster GenBank file

Download region SVG

Location: 169,663 - 210,968 nt. (total: 41,306 nt).
This entry is originally from NCBI GenBank DS570624.1.

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

General

Compounds

Genes

Biosynthesis

History

KnownClusterBlast

General information about the BGC

MIBiG accession	BGC0000845
Short description	clavulanic acid biosynthetic gene cluster from Streptomyces clavuligerus ATCC 27064
Status	Quality: questionable The quality level of this entry. Status: active The status of this entry. Completeness: complete Whether the entry covers everything needed for the pathway producing the compound(s)
Biosynthetic class(es)	other (non-nrp beta-lactam)
Loci	DS570624.1 177438 - 210968 via Enzymatic assays, Heterologous expression
Compounds	clavulanic acid
Species	Streptomyces clavuligerus ATCC 27064 [taxonomy]
References	[1] Genes specific for the biosynthesis of clavam metabolites antipodal to clavulanic acid are clustered with the gene for clavaminate synthase 1 in Streptomyces clavuligerus. Mosher RH et al., Antimicrob Agents Chemother (1999) PMID:10223939 [2] Enzymes catalyzing the early steps of clavulanic acid biosynthesis are encoded by two sets of paralogous genes in Streptomyces clavuligerus. Jensen SE et al., Antimicrob Agents Chemother (2000) PMID:10681345 [3] Applications of gene replacement technology to Streptomyces clavuligerus strain development for clavulanic acid production. Paradkar AS et al., Appl Environ Microbiol (2001) PMID:11319114 [4] The positive activator of cephamycin C and clavulanic acid production in Streptomyces clavuligerus is mistranslated in a bldA mutant. Trepanier NK et al., Microbiology (Reading) (2002) PMID:11882698 [5] Five additional genes are involved in clavulanic acid biosynthesis in Streptomyces clavuligerus. Jensen SE et al., Antimicrob Agents Chemother (2004) PMID:14693539 [6] Streptomyces clavuligerus has a second copy of the proclavaminate amidinohydrolase gene. Jensen SE et al., Antimicrob Agents Chemother (2004) PMID:14742203 [7] Two sets of paralogous genes encode the enzymes involved in the early stages of clavulanic acid and clavam metabolite biosynthesis in Streptomyces clavuligerus. Tahlan K et al., Antimicrob Agents Chemother (2004) PMID:14982786 [8] The paralogous pairs of genes involved in clavulanic acid and clavam metabolite biosynthesis are differently regulated in Streptomyces clavuligerus. Tahlan K et al., J Bacteriol (2004) PMID:15342599 [9] Expression of ccaR, encoding the positive activator of cephamycin C and clavulanic acid production in Streptomyces clavuligerus, is dependent on bldG. Bignell DR et al., Antimicrob Agents Chemother (2005) PMID:15793135 [10] Functional effects of increased copy number of the gene encoding proclavaminate amidino hydrolase on clavulanic acid production in Streptomyces clavuligerus ATCC 27064. Song JY et al., J Microbiol Biotechnol (2008) PMID:18388457 [11] A gene located downstream of the clavulanic acid gene cluster in Streptomyces clavuligerus ATCC 27064 encodes a putative response regulator that affects clavulanic acid production. Song JY et al., J Ind Microbiol Biotechnol (2009) PMID:19011915 [12] Crystal structures of an oligopeptide-binding protein from the biosynthetic pathway of the beta-lactamase inhibitor clavulanic acid. Mackenzie AK et al., J Mol Biol (2010) PMID:19941870 [13] Clavulanic acid biosynthesis and genetic manipulation for its overproduction. Song JY et al., Appl Microbiol Biotechnol (2010) PMID:20711575 [14] Carboxyethylarginine synthase genes show complex cross-regulation in Streptomyces clavuligerus. Kwong T et al., Appl Environ Microbiol (2013) PMID:23104404 [15] Cloning, sequencing and disruption of a gene from Streptomyces clavuligerus involved in clavulanic acid biosynthesis. Aidoo KA et al., Gene (1994) PMID:8088547 [16] A pathway-specific transcriptional activator regulates late steps of clavulanic acid biosynthesis in Streptomyces clavuligerus. Paradkar AS et al., Mol Microbiol (1998) PMID:9515708

Chemical products information

clavulanic acid Evidence:

Copy SMILES

C₈H₉N₁O₅

Chemical database entries
PubCHEM

List of genes involved in compound(s) production

Identifiers	Position	Product	Functions	Evidence	Extra
SSCG_00148 EDY47121.1	175791 - 177446 (-)	secreted penicillin binding protein			copy AA seq copy Nt seq
SSCG_00149 EDY47120.1	177438 - 177650 (+)	hypothetical protein			copy AA seq copy Nt seq
SSCG_00150 EDY47122.1	177939 - 179660 (+)	carboxyethylarginine synthetase	Scaffold biosynthesis	Knock-out	copy AA seq copy Nt seq
SSCG_00151 EDY47123.1	179663 - 181204 (+)	beta-lactam synthetase	Scaffold biosynthesis	Knock-out	copy AA seq copy Nt seq
SSCG_00152 EDY47124.1	181377 - 182318 (+)	proclavaminate amidinohydrolase	Scaffold biosynthesis	Knock-out	copy AA seq copy Nt seq
SSCG_00153 EDY47125.1	182334 - 183311 (+)	clavaminate synthase	Scaffold biosynthesis	Knock-out	copy AA seq copy Nt seq
SSCG_00154 EDY47126.1	183618 - 184799 (+)	glutamate N-acetyltransferase 2	Precursor biosynthesis	Knock-out	copy AA seq copy Nt seq
SSCG_00155 EDY47127.1	184964 - 186631 (+)	oligopeptide-binding lipoprotein	Transport	Knock-out	copy AA seq copy Nt seq
SSCG_00156 EDY47128.1	187415 - 188734 (-)	transcription activator	Regulation	Knock-out	copy AA seq copy Nt seq
SSCG_00157 EDY47129.1	189039 - 189785 (-)	clavaldehyde dehydrogenase	Scaffold biosynthesis	Knock-out	copy AA seq copy Nt seq
SSCG_00158 EDY47130.1	190186 - 191412 (+)	cytochrome P450-SU2			copy AA seq copy Nt seq
SSCG_00159 EDY47131.1	191418 - 191624 (+)	ferredoxin			copy AA seq copy Nt seq
SSCG_00160 EDY47132.1	191858 - 193234 (+)	beta-lactamase			copy AA seq copy Nt seq
SSCG_00161 EDY47133.1	193231 - 194253 (+)	efflux protein	Transport	Knock-out	copy AA seq copy Nt seq
SSCG_00162 EDY47134.1	194205 - 195224 (-)	acetyltransferase	Scaffold biosynthesis	Knock-out	copy AA seq copy Nt seq
SSCG_00163 EDY47135.1	195611 - 196942 (-)	oligopeptide-binding lipoprotein	Transport	Knock-out	copy AA seq copy Nt seq
SSCG_00164 EDY47136.1	197297 - 198502 (-)	conserved hypothetical protein			copy AA seq copy Nt seq
SSCG_00165 EDY47137.1	198499 - 199887 (-)	glycylclavaminate synthase	Scaffold biosynthesis	Knock-out	copy AA seq copy Nt seq
SSCG_00166 EDY47138.1	200085 - 201569 (-)	penicillin-binding protein PBP	Resistance/immunity	Other in vivo study	copy AA seq copy Nt seq
SSCG_00167 EDY47139.1	202035 - 204329 (+)	penicillin-binding protein	Resistance/immunity	Knock-out	copy AA seq copy Nt seq
SSCG_00168 EDY47140.1	204526 - 205707 (+)	cytochrome P450 hydroxylase			copy AA seq copy Nt seq
SSCG_00169 EDY47141.1	206500 - 207105 (+)	RNA polymerase sigma factor SigL	Regulation	Knock-out	copy AA seq copy Nt seq
SSCG_00170 EDY47142.1	207102 - 208652 (-)	histidine kinase	Regulation	Knock-out	copy AA seq copy Nt seq
SSCG_00171 EDY47143.1	208814 - 209626 (-)	two-component system response regulator	Regulation	Knock-out	copy AA seq copy Nt seq
SSCG_00172 EDY47144.1	210450 - 210968 (-)	transcriptional regulator			copy AA seq copy Nt seq

Biosynthesis information

No biosynthesis information available for this record.

Annotation changelog

Entry version: 4

Date

Changes

Submitters

Reviewers

2022-10-07

Update chemical activity to schema version 2.11

(ID: AAAAAAAAAAAAAAAAAAAAAAAA)

(ID: AAAAAAAAAAAAAAAAAAAAAAAA)

Entry version: 3

Date

Changes

Submitters

Reviewers

2022-09-15

Removed gene names of 'No gene ID'

(ID: AAAAAAAAAAAAAAAAAAAAAAAA)

(ID: AAAAAAAAAAAAAAAAAAAAAAAA)

2022-09-15

Updated bioactivity data

(ID: AAAAAAAAAAAAAAAAAAAAAAAA)

(ID: AAAAAAAAAAAAAAAAAAAAAAAA)

Entry version: 2

Date

Changes

Submitters

Reviewers

2019-10-16

Migrated from v1.4

(ID: AAAAAAAAAAAAAAAAAAAAAAAA)

(ID: AAAAAAAAAAAAAAAAAAAAAAAA)

Entry version: 1

Date

Changes

Submitters

Reviewers

2015-06-12

Submitted

(ID: SDHXYXULZ6ZTMHSITTYHRAHA)

(ID: AAAAAAAAAAAAAAAAAAAAAAAA)

Similar known clusters from MIBiG 4.0