BGC0000845

BGC0000845: clavulanic acid biosynthetic gene cluster from Streptomyces clavuligerus ATCC 27064

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Location: 177,438 - 210,968 nt. (total: 33,531 nt).
This entry is originally from NCBI GenBank DS570624.1.

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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General

Compounds

Genes

History

KnownClusterBlast

General information about the BGC

MIBiG accession	BGC0000845
Short description	clavulanic acid biosynthetic gene cluster from Streptomyces clavuligerus ATCC 27064
Status	Minimal annotation: no A minimal annotation only contains information on the BGC loci and one or more linked chemical product(s) Completeness: complete Whether the loci encodes everything needed for the pathway producing the compound(s)
Biosynthetic class(es)	Other (Non-NRP beta-lactam)
Loci	NCBI GenBank: DS570624.1
Compounds	clavulanic acid
Species	Streptomyces clavuligerus ATCC 27064 [taxonomy]
References	Cloning, sequencing and disruption of a gene from Streptomyces clavuligerus involved in clavulanic acid biosynthesis. Aidoo KA et al., Gene (1994) PMID:8088547 A pathway-specific transcriptional activator regulates late steps of clavulanic acid biosynthesis in Streptomyces clavuligerus. Paradkar AS et al., Mol Microbiol (1998) PMID:9515708 Genes specific for the biosynthesis of clavam metabolites antipodal to clavulanic acid are clustered with the gene for clavaminate synthase 1 in Streptomyces clavuligerus. Mosher RH et al., Antimicrob Agents Chemother (1999) PMID:10223939 Enzymes catalyzing the early steps of clavulanic acid biosynthesis are encoded by two sets of paralogous genes in Streptomyces clavuligerus. Jensen SE et al., Antimicrob Agents Chemother (2000) PMID:10681345 Applications of gene replacement technology to Streptomyces clavuligerus strain development for clavulanic acid production. Paradkar AS et al., Appl Environ Microbiol (2001) PMID:11319114 The positive activator of cephamycin C and clavulanic acid production in Streptomyces clavuligerus is mistranslated in a bldA mutant. Trepanier NK et al., Microbiology (Reading) (2002) PMID:11882698 Five additional genes are involved in clavulanic acid biosynthesis in Streptomyces clavuligerus. Jensen SE et al., Antimicrob Agents Chemother (2004) PMID:14693539 Streptomyces clavuligerus has a second copy of the proclavaminate amidinohydrolase gene. Jensen SE et al., Antimicrob Agents Chemother (2004) PMID:14742203 Two sets of paralogous genes encode the enzymes involved in the early stages of clavulanic acid and clavam metabolite biosynthesis in Streptomyces clavuligerus. Tahlan K et al., Antimicrob Agents Chemother (2004) PMID:14982786 The paralogous pairs of genes involved in clavulanic acid and clavam metabolite biosynthesis are differently regulated in Streptomyces clavuligerus. Tahlan K et al., J Bacteriol (2004) PMID:15342599 Expression of ccaR, encoding the positive activator of cephamycin C and clavulanic acid production in Streptomyces clavuligerus, is dependent on bldG. Bignell DR et al., Antimicrob Agents Chemother (2005) PMID:15793135 Functional effects of increased copy number of the gene encoding proclavaminate amidino hydrolase on clavulanic acid production in Streptomyces clavuligerus ATCC 27064. Song JY et al., J Microbiol Biotechnol (2008) PMID:18388457 A gene located downstream of the clavulanic acid gene cluster in Streptomyces clavuligerus ATCC 27064 encodes a putative response regulator that affects clavulanic acid production. Song JY et al., J Ind Microbiol Biotechnol (2009) PMID:19011915 Crystal structures of an oligopeptide-binding protein from the biosynthetic pathway of the beta-lactamase inhibitor clavulanic acid. Mackenzie AK et al., J Mol Biol (2010) PMID:19941870 Clavulanic acid biosynthesis and genetic manipulation for its overproduction. Song JY et al., Appl Microbiol Biotechnol (2010) PMID:20711575 Carboxyethylarginine synthase genes show complex cross-regulation in Streptomyces clavuligerus. Kwong T et al., Appl Environ Microbiol (2013) PMID:23104404

Chemical products information

clavulanic acid

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C₈H₉N₁O₅

Chemical database entries
PubCHEM

List of genes involved in compound(s) production

Identifiers	Position	Product	Functions	Evidence	Extra
SSCG_00149 EDY47120.1	177438 - 177650 (+)	hypothetical protein			copy AA seq copy Nt seq
SSCG_00150 EDY47122.1	177939 - 179660 (+)	carboxyethylarginine synthetase	Scaffold biosynthesis	Knock-out	copy AA seq copy Nt seq
SSCG_00151 EDY47123.1	179663 - 181204 (+)	beta-lactam synthetase	Scaffold biosynthesis	Knock-out	copy AA seq copy Nt seq
SSCG_00152 EDY47124.1	181377 - 182318 (+)	proclavaminate amidinohydrolase	Scaffold biosynthesis	Knock-out	copy AA seq copy Nt seq
SSCG_00153 EDY47125.1	182334 - 183311 (+)	clavaminate synthase	Scaffold biosynthesis	Knock-out	copy AA seq copy Nt seq
SSCG_00154 EDY47126.1	183618 - 184799 (+)	glutamate N-acetyltransferase 2	Precursor biosynthesis	Knock-out	copy AA seq copy Nt seq
SSCG_00155 EDY47127.1	184964 - 186631 (+)	oligopeptide-binding lipoprotein	Transport	Knock-out	copy AA seq copy Nt seq
SSCG_00156 EDY47128.1	187415 - 188734 (-)	transcription activator	Regulation	Knock-out	copy AA seq copy Nt seq
SSCG_00157 EDY47129.1	189039 - 189785 (-)	clavaldehyde dehydrogenase	Scaffold biosynthesis	Knock-out	copy AA seq copy Nt seq
SSCG_00158 EDY47130.1	190186 - 191412 (+)	cytochrome P450-SU2	Unknown	Knock-out	copy AA seq copy Nt seq
SSCG_00159 EDY47131.1	191418 - 191624 (+)	ferredoxin	Unknown	Knock-out	copy AA seq copy Nt seq
SSCG_00160 EDY47132.1	191858 - 193234 (+)	beta-lactamase	Unknown	Knock-out	copy AA seq copy Nt seq
SSCG_00161 EDY47133.1	193231 - 194253 (+)	efflux protein	Transport	Knock-out	copy AA seq copy Nt seq
SSCG_00162 EDY47134.1	194205 - 195224 (-)	acetyltransferase	Scaffold biosynthesis	Knock-out	copy AA seq copy Nt seq
SSCG_00163 EDY47135.1	195611 - 196942 (-)	oligopeptide-binding lipoprotein	Transport	Knock-out	copy AA seq copy Nt seq
SSCG_00164 EDY47136.1	197297 - 198502 (-)	conserved hypothetical protein	Unknown	Knock-out	copy AA seq copy Nt seq
SSCG_00165 EDY47137.1	198499 - 199887 (-)	glycylclavaminate synthase	Scaffold biosynthesis	Knock-out	copy AA seq copy Nt seq
SSCG_00166 EDY47138.1	200085 - 201569 (-)	penicillin-binding protein PBP	Resistance/immunity	Other in vivo study	copy AA seq copy Nt seq
SSCG_00167 EDY47139.1	202035 - 204329 (+)	penicillin-binding protein	Resistance/immunity	Knock-out	copy AA seq copy Nt seq
SSCG_00168 EDY47140.1	204526 - 205707 (+)	cytochrome P450 hydroxylase	Unknown	Knock-out	copy AA seq copy Nt seq
SSCG_00169 EDY47141.1	206500 - 207105 (+)	RNA polymerase sigma factor SigL	Regulation	Knock-out	copy AA seq copy Nt seq
SSCG_00170 EDY47142.1	207102 - 208652 (-)	histidine kinase	Regulation	Knock-out	copy AA seq copy Nt seq
SSCG_00171 EDY47143.1	208814 - 209626 (-)	two-component system response regulator	Regulation	Knock-out	copy AA seq copy Nt seq
SSCG_00172 EDY47144.1	210450 - 210968 (-)	transcriptional regulator			copy AA seq copy Nt seq

Annotation changelog

MIBiG version	Submitter	Notes
1.0	Hidden contributor (ID: SDHXYXULZ6ZTMHSITTYHRAHA, no GDPR consent given).	Submitted
2.0	Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).	Migrated from v1.4
3.0	Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).	Removed gene names of 'No gene ID' Updated bioactivity data
3.1	Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).	Update chemical activity to schema version 2.11

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