Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0000845.1:
Cluster 1Other133531

BGC0000845, clavulanic acid biosynthetic gene cluster from Streptomyces clavuligerus. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: clavulanic acid
PubChem ID: 5280980
SMILES string: Copy to clipboard
Molecular formula: C8H9NO5
Average molecular mass: 199.162 Da
Molecular activity: Inhibitor
Molecular target: beta-lactamase

Class-specific details

Biosynthetic class(es):
Other
Other biosynthetic class:
Non-NRP beta-lactam

Gene cluster description

clavulanic acid (BGC0000845). Gene Cluster 1. Biosynthetic class = Other. GenBank DS570624, positions 177438-209626. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Homologous known gene clusters

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Enzymatic assays, Heterologous expression, Sequence-based prediction
MIxS-compliance:Unknown
Contact for this cluster:Susan Jensen (University of Alberta)

Literature references

1. Aidoo KA et al. (1994) Cloning, sequencing and disruption of a gene from Streptomyces clavuligerus involved in clavulanic acid biosynthesis. Gene 147(1):41-6.
2. Paradkar AS et al. (1998) A pathway-specific transcriptional activator regulates late steps of clavulanic acid biosynthesis in Streptomyces clavuligerus. Mol Microbiol 27(4):831-43.
3. Mosher RH et al. (1999) Genes specific for the biosynthesis of clavam metabolites antipodal to clavulanic acid are clustered with the gene for clavaminate synthase 1 in Streptomyces clavuligerus. Antimicrob Agents Chemother 43(5):1215-24.
4. Jensen SE et al. (2000) Enzymes catalyzing the early steps of clavulanic acid biosynthesis are encoded by two sets of paralogous genes in Streptomyces clavuligerus. Antimicrob Agents Chemother 44(3):720-6.
5. Paradkar AS et al. (2001) Applications of gene replacement technology to Streptomyces clavuligerus strain development for clavulanic acid production. Appl Environ Microbiol 67(5):2292-7. doi:
6. Trepanier NK et al. (2002) The positive activator of cephamycin C and clavulanic acid production in Streptomyces clavuligerus is mistranslated in a bldA mutant. Microbiology 148(Pt 3):643-56. doi: 10.1099/00221287-148-3-643.
7. Jensen SE et al. (2004) Five additional genes are involved in clavulanic acid biosynthesis in Streptomyces clavuligerus. Antimicrob Agents Chemother 48(1):192-202.
8. Jensen SE et al. (2004) Streptomyces clavuligerus has a second copy of the proclavaminate amidinohydrolase gene. Antimicrob Agents Chemother 48(2):514-20.
9. Tahlan K et al. (2004) Two sets of paralogous genes encode the enzymes involved in the early stages of clavulanic acid and clavam metabolite biosynthesis in Streptomyces clavuligerus. Antimicrob Agents Chemother 48(3):930-9.
10. Tahlan K et al. (2004) The paralogous pairs of genes involved in clavulanic acid and clavam metabolite biosynthesis are differently regulated in Streptomyces clavuligerus. J Bacteriol 186(18):6286-97. doi: 10.1128/JB.186.18.6286-6297.2004.
11. Bignell DR et al. (2005) Expression of ccaR, encoding the positive activator of cephamycin C and clavulanic acid production in Streptomyces clavuligerus, is dependent on bldG. Antimicrob Agents Chemother 49(4):1529-41. doi:
12. Song JY et al. (2008) Functional effects of increased copy number of the gene encoding proclavaminate amidino hydrolase on clavulanic acid production in Streptomyces clavuligerus ATCC 27064. J Microbiol Biotechnol 18(3):417-26.
13. Song JY et al. (2009) A gene located downstream of the clavulanic acid gene cluster in Streptomyces clavuligerus ATCC 27064 encodes a putative response regulator that affects clavulanic acid production. J Ind Microbiol Biotechnol 36(2):301-11. doi:
14. Mackenzie AK et al. (2010) Crystal structures of an oligopeptide-binding protein from the biosynthetic pathway of the beta-lactamase inhibitor clavulanic acid. J Mol Biol 396(2):332-44. doi: 10.1016/j.jmb.2009.11.045. Epub 2009
15. Song JY et al. (2010) Clavulanic acid biosynthesis and genetic manipulation for its overproduction. Appl Microbiol Biotechnol 88(3):659-69. doi: 10.1007/s00253-010-2801-2.
16. Kwong T et al. (2013) Carboxyethylarginine synthase genes show complex cross-regulation in Streptomyces clavuligerus. Appl Environ Microbiol 79(1):240-9. doi: 10.1128/AEM.02600-12. Epub