LOCUS       BGC0000850              2474 bp    DNA     linear   BCT 15-JUL-2009
DEFINITION  Streptomyces acidiscabies 84.104 GBL gene cluster, complete
            sequence.
ACCESSION   BGC0000850
VERSION     BGC0000850
KEYWORDS    .
SOURCE      Streptomyces acidiscabies
  ORGANISM  Streptomyces acidiscabies
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 14:19:05
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     gene            complement(1..648)
                     /gene="sabR"
     CDS             complement(1..648)
                     /codon_start=1
                     /gene="sabR"
                     /gene_functions="regulatory (smcogs) SMCOG1224:TetR family
                     transcriptional regulator (Score: 59.9; E-value: 4.9e-18)"
                     /gene_kind="regulatory"
                     /note="similar to GBL receptor"
                     /product="SabR"
                     /protein_id="ACO51011.1"
                     /transl_table=11
                     /translation="MAKQERAIRTRQAIVEAAGEVFDEHGYTSTTIAMVLERSAVTKGA
                     LYFHFPSKEALAQAVLGEQVPFGSVPAQACKLQEIVDMTFVVGQRLLTSSLLRGSIRLA
                     VDQEAPARIDHGGPYRQWSQRLEDLLEQARDRGELLPTVQPRQTVELLVGCFSGIQLMS
                     RALSGRADLGERLSVLWTHILPSIAVPGLLPALDTKADRGVRVLGSPERVAS"
     source          1..2474
                     /db_xref="taxon:42234"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces acidiscabies"
                     /strain="84.104"
     misc_feature    1..2474
                     /note="GBL gene cluster"
     protocluster    1..2474
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[737:1745]"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      738..1745
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     cand_cluster    1..2474
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..2474
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000850"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..2474
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="butyrolactone"
                     /region_number="1"
                     /rules="AfsA"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            738..1745
                     /gene="sabA"
     CDS             738..1745
                     /codon_start=1
                     /gene="sabA"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /note="similar to GBL synthase"
                     /product="SabA"
                     /protein_id="ACO51012.1"
                     /sec_met_domain="AfsA (E-value: 7.5e-25, bitscore: 74.7,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPNRSPRTLTFAVGTIHRPPWLMGDRREDAMTRPRQSADTPSLTA
                     TVPRQFVHRAALAETFLTGWERTEADRFTVCAQWPRAHGLHVSPDWCSYDPLLVVETVR
                     QAGTLIMHTEYGVPLDRQFVLNAFEVTTDPHRLEVGPVPAEPTVELDFTEVRYRGGQPA
                     ACRYSARVLCCGEQAATADVAFTCVGEAVYRRLRGGRTPGTVTAVPLPPGLPPQVVDRA
                     LPTDVVLALPGLDPGAPGGSWQLRVNTAHPVFFDHPLDHVPGMLLLEAARQAARFALPG
                     KYAPSAYRAVFHRYAELDEPVWIEARDEGGGHLQVVGVQRESAVFECGVDAAAG"
     misc_feature    990..992
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     protein_bind    1768..1793
                     /bound_moiety="SabR"
                     /note="ARE binding site for SabR"
     gene            1779..2474
                     /gene="sabS"
     CDS             1779..2474
                     /codon_start=1
                     /gene="sabS"
                     /gene_functions="regulatory (smcogs) SMCOG1057:TetR family
                     transcriptional regulator (Score: 79.3; E-value: 8.4e-24)"
                     /gene_kind="regulatory"
                     /note="GBL receptor"
                     /product="SabS"
                     /protein_id="ACO51013.1"
                     /transl_table=11
                     /translation="MTRSFTGPGVSQAMARQLRAEQTRATIITAAADLFDRHGYESTSL
                     SDIVEHAQVTKGALYFHFAAKEDLAHAIMELQSQASQRVAGEIDNRGYSSLEALMRLTF
                     GLTRLSVEGPIARAGLRLATGGVAVRPPLRRPFSEWGELINRGLSGAVREADIHQDVDI
                     EAVAHSLVCFFVGTRVVGRSREASGRLPRRMAEMWYVLIRGLVPVPRRPRYLSLASRLE
                     REITAACVS"
     misc_feature    1818
                     /gene="sabS"
                     /note="possible alternate site of translation initiation"
     misc_feature    1830..1832
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
ORIGIN
        1 tcacgaagcc accctttcgg gcgaccccag gacccgcacg ccccggtccg ccttcgtgtc
       61 cagcgcgggc agcagccccg gtacggcgat gctcggcagg atgtgcgtcc acagcaccga
      121 cagccgctcg ccgaggtccg cgcgcccgga cagcgcccgg gacatcagct ggatcccgct
      181 gaagcagccg acgagcagct ccaccgtctg ccggggctgt accgtcggca gcaactcccc
      241 ccggtcccgg gcctgttcga gcaggtcctc cagccgctgg gaccactgcc ggtacggccc
      301 tccgtgatcg atgcgggccg gcgcctcctg gtcgaccgcg aggcggatgc tccctctcaa
      361 caaggaactg gtcagcaggc gttggccgac gacgaacgtc atgtcgacga tctcctggag
      421 cttgcacgcc tgtgccggga cgctcccgaa cggcacctgc tcgcccagca ccgcctgcgc
      481 cagcgcctcc ttggacggga agtggaagta cagcgcgccc ttggtgacgg cggagcgctc
      541 caggaccatg gcgatggtgg tggacgtgta accgtgctcg tcgaaaacct caccggccgc
      601 ttccacgatg gcttggcgcg ttctgatggc gcgctcctgt ttagccataa gagcccctcc
      661 gcgggaccga gttggccgat cccgcgatcg tgtgtctgtt cggcccgatc ataggatgcc
      721 gtcgactgat ctcattgatg ccaaaccggt cgcctcgtac tctaacgttc gctgtgggga
      781 cgattcaccg tccgccgtgg ctcatgggtg accgcaggga ggatgcgatg acccgaccgc
      841 ggcagtctgc tgacaccccg tccctgacgg caaccgtccc tcgccagttc gtccaccgcg
      901 cagccctcgc cgaaacgttt ctcaccggct gggagcgcac cgaggccgac cgtttcaccg
      961 tgtgcgcaca gtggccccgt gcccatgggt tacacgtgtc cccggactgg tgctcctacg
     1021 accccttgct cgtggtggag acggtccgtc aggccggcac cctgatcatg cacaccgagt
     1081 acggcgtccc gctggaccgg cagttcgtgc tcaacgcctt cgaagtcacc acggatccgc
     1141 accggttgga ggtcgggccg gtgccggccg agccgaccgt cgaactcgac ttcaccgagg
     1201 tgcgctaccg gggcggccag cctgccgcct gccgctactc cgcccgcgtc ctgtgctgcg
     1261 gggagcaggc cgcgaccgcc gacgtcgcct tcacctgcgt cggcgaggcc gtctaccgca
     1321 ggctgcgcgg cggccggacg cccggcacgg tgacggccgt tccgctgccg cccgggctgc
     1381 cgccgcaggt cgtggaccgg gcactgccca ccgacgtcgt cctcgccctc cccggcctcg
     1441 atccgggggc gcccggcggg agttggcagc tccgggtaaa taccgcacac cccgttttct
     1501 tcgaccatcc gctcgatcac gtccccggga tgctcctgct ggaggcggcc cggcaggcag
     1561 cccggttcgc tcttccgggc aaatatgccc ccagcgcata ccgggcggtc ttccacaggt
     1621 acgctgagct ggacgaaccc gtctggatcg aggctcgcga cgaaggcggc ggacacctcc
     1681 aagtcgtcgg cgtgcaacgg gagtcggcag tcttcgagtg tggggtcgac gccgccgcag
     1741 ggtgagttat tgtgtacagg gggtgagaaa caaacggcat gacccgttct tttaccggcc
     1801 caggagtgtc tcaggcgatg gcgaggcagt tacgcgccga acagacccgc gcgacgatca
     1861 tcacggcggc ggccgatctg ttcgaccgac atggttacga atcgacgagc ctcagtgaca
     1921 tcgtcgagca cgcccaagtc accaagggcg ccctgtactt ccacttcgcg gccaaggagg
     1981 acctggccca cgcgatcatg gaactccagt cccaggcctc gcagcgcgtc gcgggcgaga
     2041 tcgacaaccg gggctactcc tccctcgaag cgctgatgcg gctcacgttc ggcctgaccc
     2101 ggctgtccgt cgaagggccc atcgcccggg ccgggttgag gctcgcgacc gggggagtcg
     2161 cggtgagacc cccgctgcgc cgccccttct ccgagtgggg tgagctgatc aaccgcgggc
     2221 tgtccggggc ggtccgtgag gccgacatac accaggacgt cgacatcgag gcggtcgcgc
     2281 actccctggt ctgcttcttc gtcggcaccc gcgtggtggg ccgctcccgc gaggcctccg
     2341 gccggcttcc gcgccggatg gcggagatgt ggtacgtcct gatccgcggc ctcgtcccgg
     2401 tcccgcgcag accgcgctat ctgagcctgg cgtcgaggct ggagcgggag atcacggcgg
     2461 cgtgcgtgtc ctga
//
