LOCUS       BGC0000852              3537 bp    DNA     linear   BCT 04-FEB-2002
DEFINITION  Bacillus pasteurii osmotically regulated ectoine biosynthesis gene
            cluster, complete sequence.
ACCESSION   BGC0000852
VERSION     BGC0000852
KEYWORDS    .
SOURCE      Sporosarcina pasteurii
  ORGANISM  Sporosarcina pasteurii
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 14:19:21
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3537
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /db_xref="ATCC:11859"
                     /db_xref="taxon:1474"
                     /mol_type="genomic DNA"
                     /note="type strain"
                     /organism="Sporosarcina pasteurii"
                     /strain="ATCC 11859"
                     /type_material="type strain of Sporosarcina pasteurii"
     protocluster    1..3537
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[2449:2854]"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      2450..2854
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     cand_cluster    1..3537
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..3537
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000852"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..3537
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            416..958
                     /gene="ectA"
     CDS             416..958
                     /codon_start=1
                     /gene="ectA"
                     /note="similar to acetyltransferase"
                     /product="EctA"
                     /protein_id="AAK12083.1"
                     /transl_table=11
                     /translation="MFWVISKQGSTAVAEQEETLVFRVPTEDDGKAIWNLINYPGVLDL
                     LSSYSYFMWAKFFDQTSVVGETNEQIVGFYIGLHTTEYGPDTLFYLASCSDETQRQKGL
                     ASRMLQAILHRYAWRNIRYLEATVGTSNEAPEALFQKLSRDLKTAYHVTEFFTEDQFPG
                     KGHEDERLFKIGPFQQV"
     gene            1010..2290
                     /gene="ectB"
     CDS             1010..2290
                     /NRPS_PKS="Domain: Aminotran_3 (30-364). E-value: 7.1e-79.
                     Score: 257.1. Matches aSDomain:
                     nrpspksdomains_ectB_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013:aminotransferase class-III (Score: 369.3;
                     E-value: 4e-112)"
                     /gene_kind="biosynthetic-additional"
                     /note="similar to transaminase"
                     /product="EctB"
                     /protein_id="AAK12084.1"
                     /sec_met_domain="Aminotran_3 (E-value: 1.1e-83, bitscore:
                     268.8, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLLTKEKNGMEIIEERESAVRSYSRSFPTVFEKAKDHLVWDVDGK
                     EYIDFFAGAGSLNYGHNNEKMKTKIMDYVMNDGISHSLDMGTVARAEFLETFNEVILRP
                     RNLDYKVMFPGPTGTNTVESALKIARKVTGRQNIISFTNAFHGMTLGSLSISGNSSIRN
                     GAGVPLTNTISMPYDTFFKNGNAIDYLEQYLEDTGSGVDLPAAMILETVQGEGGINAAS
                     FEWLRGIEKLCRRYDILLIIDDVQAGCGRTGTFFSFEPAGIQPDIVCLSKSIGGYGLPL
                     AITLIKPEHDIWEPGEHNGTFRGNNMAIVAATEALSYWKTDDLAKSVQKKSKIIKLRFE
                     QIVEDYPELKATTRGRGFMQGIACGKGKEAYATKICAKAFEKGVIMETSGPSGEVVKFL
                     GALTIDETSLIKGLGILEEATEEVVRQ"
     aSDomain        1100..2101
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ectB_Aminotran_3.1"
                     /evalue="7.10E-79"
                     /label="ectB_Aminotran_3.1"
                     /locus_tag="ectB"
                     /protein_end="364"
                     /protein_start="30"
                     /score="257.1"
                     /tool="antismash"
                     /translation="FEKAKDHLVWDVDGKEYIDFFAGAGSLNYGHNNEKMKTKIMDYVM
                     NDGISHSLDMGTVARAEFLETFNEVILRPRNLDYKVMFPGPTGTNTVESALKIARKVTG
                     RQNIISFTNAFHGMTLGSLSISGNSSIRNGAGVPLTNTISMPYDTFFKNGNAIDYLEQY
                     LEDTGSGVDLPAAMILETVQGEGGINAASFEWLRGIEKLCRRYDILLIIDDVQAGCGRT
                     GTFFSFEPAGIQPDIVCLSKSIGGYGLPLAITLIKPEHDIWEPGEHNGTFRGNNMAIVA
                     ATEALSYWKTDDLAKSVQKKSKIIKLRFEQIVEDYPELKATTRGRGFMQGIAC"
     gene            2450..2854
                     /gene="ectC"
     CDS             2450..2854
                     /codon_start=1
                     /gene="ectC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /note="similar to ectoine synthase"
                     /product="EctC"
                     /protein_id="AAK12085.1"
                     /sec_met_domain="ectoine_synt (E-value: 2.1e-52, bitscore:
                     164.2, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRTIDEIIGTENEVESDTWTSRRLLLEKDGMGFSFHETIIYAG
                     TETHIHYQNHLEAVYCVGGDGEIETVSDGKVYPIQDGTMYALDQHDEHYPRGGKTDMRL
                     ICTFNPPLVGTETHDENGVYPLLSKQPVGK"
ORIGIN
        1 gaattccttt tgcaaggcgc taatttttgc tcttcatact gcaaggcaga aaacagccca
       61 atgatgatgt cgccagcaat tcctaagcgg gcgataatca aaagtcacca attacccttg
      121 ttatatccac tttataattc ttcatgagga cttatcggga atgtaaaaac aatccttgat
      181 cagccaccct ctctaaaatt ctcttatctt gatacgatta acatcgtact gcgggaacga
      241 tcttttcaca tgaataaatt ctcaagtttc ataggatcaa caatgggtta cttcaattgt
      301 ttagaaggtg taaataattc ctgctaatat gtgggtgaaa agcactgaag tcaaacttca
      361 tcaaaaaaac gccaaggtga aacattcgta atggtgggtc ggacttggga ggtgaatgtt
      421 ttgggtaata agtaagcagg gatcaacagc agtcgctgag caagaagaga ccctggtctt
      481 cagagtacca acggaagatg acggcaaagc aatatggaat cttattaact atcccggtgt
      541 actcgattta ctatcttcgt atagttattt tatgtgggcg aagttttttg accaaaccag
      601 tgtagttggt gaaacaaacg aacaaattgt cggtttttat atcgggcttc atacaaccga
      661 atacggccca gatacgcttt tttatttggc aagttgcagc gatgaaaccc agcgacagaa
      721 agggcttgct tcaaggatgt tgcaagcgat tttgcaccgc tatgcatggc ggaatatccg
      781 gtatttggag gcgactgtag ggacttcaaa cgaagcaccc gaagccttat ttcaaaagtt
      841 atcaagagac ttgaagacgg cgtaccatgt gacagaattt tttactgaag accagtttcc
      901 aggaaaggga cacgaagacg aacggttatt caaaatcggc ccctttcagc aagtatgaaa
      961 agatgagaac aaaataattg aacgtaaaaa gtggaggaat gtcaaaccaa tgttattaac
     1021 taaagaaaaa aatggtatgg aaattattga agaacgtgag tcagcagtca gaagttatag
     1081 ccgtagtttt ccaacagtat ttgagaaagc gaaggatcat ttggtgtggg atgtagacgg
     1141 taaagagtat atcgatttct tcgcaggtgc aggttcactg aactatggcc ataataatga
     1201 gaagatgaag acgaaaatca tggactatgt gatgaatgat ggcatctcac atagcctgga
     1261 tatggggacg gttgcccggg ctgaatttct cgagacattt aacgaagtta ttttaaggcc
     1321 acggaattta gattataaag tgatgttccc aggtccaact ggaacaaaca cagtcgaaag
     1381 tgccttaaag attgcaagga aggtaactgg acgtcagaac attataagct ttacaaatgc
     1441 ttttcatggt atgacgttgg gttcgctatc tatctcggga aattcatcaa ttcgtaacgg
     1501 agccggggtc ccgctgacaa acacgatttc catgccgtat gatactttct ttaaaaatgg
     1561 caatgcaatt gattatcttg aacagtattt ggaggatacc ggaagtgggg tagacctgcc
     1621 ggcggccatg atcctagaaa cagttcaagg ggaaggcggc atcaacgctg caagctttga
     1681 atggttacga gggattgaaa agttatgccg tcgttatgat attttgctca ttatcgatga
     1741 tgtgcaagcg gggtgtggtc gaaccggcac gttctttagc tttgaacccg caggcatcca
     1801 acctgatatc gtttgcttgt ctaagtcaat tggtgggtat ggtttgccat tagccatcac
     1861 gttaattaaa ccagaacatg atatatggga gccaggcgaa cataatggca catttcgtgg
     1921 taataatatg gcaatcgtcg ctgcgacaga agccttatct tactggaaaa cagacgactt
     1981 agcgaaatct gttcaaaaga aatctaagat tataaaattg cgtttcgagc aaatcgttga
     2041 agattatcca gaattaaaag cgacaacacg tggccgaggt tttatgcaag gcatcgcatg
     2101 tggaaaagga aaagaggcat atgcaacaaa aatttgtgcg aaagcttttg aaaaaggggt
     2161 catcatggaa acgtcaggac ctagcgggga agtcgtgaaa tttctaggcg cgttaacaat
     2221 tgacgaaacg agtttaatca aaggcttagg tattttagaa gaagcaactg aagaagtcgt
     2281 tcgtcaataa ggatagatga tgtgtgaatg gattttgtat caacgtgtag gtcggataag
     2341 tcaggacacg tcctgctcgt gataagttgc ctacctcaaa actttaggtg ggggataggg
     2401 taaccccact cattaaattt ttttaactta caaagggaga gatgaagaaa tgatcgttcg
     2461 aacgattgat gaaatcatag gaacagaaaa tgaagtggaa tcggacacat ggacgagtcg
     2521 ccgtttacta cttgaaaaag atggcatggg cttttcattt catgaaacga ttatctatgc
     2581 aggtacagaa acgcatatcc actatcaaaa ccatttagaa gcggtgtact gtgtcggtgg
     2641 agatggagag attgaaacgg tctcggatgg aaaagtgtat ccgattcaag acggaacgat
     2701 gtatgcattg gatcaacatg atgagcatta tccccgcggg ggaaaaacgg atatgaggct
     2761 aatttgtaca ttcaatccac cgctcgtcgg taccgaaaca catgatgaaa atggtgtgta
     2821 cccacttctt tcgaaacaac ctgttggaaa ataaggtgag ttttgatcaa atgtcctata
     2881 ttgtcatcat aggttgtaca gagattcctt acgctaattg tctgagggaa cactggaata
     2941 aaatagcctt tattgttaac aataaaggct ttttatttct tgaataaacg agttccttct
     3001 atttatatag tgctgatgat cgcactggca ctgtaggaat atattaccgt tgaaaatagc
     3061 gctcctttat tataagatac taatacaagc tgcattgttc gtatcattat taagttttaa
     3121 cctttaagct gtaaaaggga gttttatgat tgaaactgaa atggcaagcc tcgttctttt
     3181 aattaacaac gaggacagac aggcaagttt ctgcgaacac ccactttgag gagtggtggt
     3241 ttaagacttt ctagagacgt acggcagagg cggctgatac ataattcata ataaatatgc
     3301 taactatata taagcatcct ccgaaactca cacaaatggt ccgttatgca attcaacatt
     3361 gatggcggac aatccatttc ataattataa ataagtgcga aaatcaccaa cgttaattac
     3421 tcaaaaatat accccccttc aaacttttgg caaaaaaagt tcgaaggggg gatttgcatt
     3481 tgcactaaaa agtattatga ttcgtaatga agaaatgaaa gaatatacat tgaattc
//
