LOCUS       BGC0000854              6907 bp    DNA     linear   BCT 21-APR-2009
DEFINITION  Methylobacter alcaliphilus ectoine biosynthesis gene cluster,
            complete sequence; and putative ectoine hydroxylase (phy) gene,
            complete cds.
ACCESSION   BGC0000854
VERSION     BGC0000854
KEYWORDS    .
SOURCE      Methylotuvimicrobium alcaliphilum 20Z
  ORGANISM  Methylotuvimicrobium alcaliphilum 20Z
            Bacteria; Pseudomonadota; Gammaproteobacteria; Methylococcales;
            Methylococcaceae; Methylotuvimicrobium.
COMMENT     On Apr 21, 2009 this sequence version replaced DQ016501.1.
            ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2024-10-17 14:19:23
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6907
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..6907
                     /db_xref="taxon:1091494"
                     /mol_type="genomic DNA"
                     /organism="Methylotuvimicrobium alcaliphilum 20Z"
                     /strain="20Z"
                     /type_material="type strain of Methylomicrobium
                     alcaliphilum"
     subregion       1..6907
                     /aStool="mibig"
                     /contig_edge="True"
                     /label="other"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..6907
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            complement(839..1381)
                     /gene="ectR"
     CDS             complement(839..1381)
                     /codon_start=1
                     /gene="ectR"
                     /gene_functions="regulatory (smcogs) SMCOG1135:MarR family
                     transcriptional regulator (Score: 76.5; E-value: 2.4e-23)"
                     /gene_kind="regulatory"
                     /note="transcriptional regulator of ectoine biosynthesis
                     genes"
                     /product="transcriptional regulator MarR family"
                     /protein_id="ACP19701.1"
                     /transl_table=11
                     /translation="MMQNPQPHAPHSLDTLDLNPVEKEHLLNQIEEVLVALRRVIRATD
                     LHSKYLAKTTSLTAPQILLLQTLRAKGQLTIGELAQDMSLSQATVTTILDRLEKRQLVF
                     RQRSQTDKRKVHVYMTEAATEMLINAPIPLQDRFTREFSKLQEWEQLMIIASLQRVAQM
                     MDAQNIPVAKEAFDFPV"
     misc_feature    839..5559
                     /note="ectoine biosynthesis gene cluster"
     gene            1709..2227
                     /gene="ectA"
     CDS             1709..2227
                     /codon_start=1
                     /function="catalyzes the acetylation of
                     L-2,4-diaminobutyrate (DABA) to gamma-N-acetyl-alpha,
                     gamma-diaminobutyric acid (ADABA) with acetyl coenzyme A"
                     /gene="ectA"
                     /product="L-2,4-diaminobutyrat acetyltransferase"
                     /protein_id="AAY96770.1"
                     /transl_table=11
                     /translation="MLPDKTALPIITLSQPTAEVGAQVHRLISKCPPLDPNSMYCNLLQ
                     SSHFSETAVAAKIGDELVGFVSGYRIPQRPDTLFVWQVAVGEKARGQGLATRMLKAILA
                     RPVNQDINRIETTITPNNKASWALFEGLAKKLDTQIGSAVMFDKTRHFADQHETEMLVK
                     VGPFKAVQA"
     gene            2270..3601
                     /gene="ectB"
     CDS             2270..3601
                     /NRPS_PKS="Domain: Aminotran_3 (21-358). E-value: 6.8e-79.
                     Score: 257.1. Matches aSDomain:
                     nrpspksdomains_ectB_Aminotran_3.1"
                     /codon_start=1
                     /function="catalyzes the conversion of aspartate
                     semialdehyde into L-2,4-diaminobutyric acid"
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013:aminotransferase class-III (Score: 384; E-value:
                     1.4e-116)"
                     /gene_kind="biosynthetic-additional"
                     /product="L-2,4-diaminobutyrat transaminase"
                     /protein_id="AAY96771.1"
                     /sec_met_domain="Aminotran_3 (E-value: 3.8e-85, bitscore:
                     274.6, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKIFEEIESEVRSYARAFPRVFDRAQGAKIYDHEGNEYLDFLAGA
                     GSLNYGHNHPVFKEKLLEYIQNDGITQGLDLHTRAKGEFLESFNEHILKPRNLDYIVMF
                     TGPTGTNAVEAALKIARKKTGRENIISFTNGWHGQTLGSLSVTGNSTHRGGAGIALHGS
                     TRIPYDGYLGDDFDTTNLLDKMLSDSSSGVDKPAAVIVETVQGEGGINAASMTWLRSLS
                     EICKRHDVLLIIDDIQAGCGRTGTFFSFEEAGIYPDIVTLSKSLSGYGLPLAIVLMKPE
                     IDQWSPGEHNGTFRGNNHAFVTAKAAIDHFWKDDSFAKDVQRKGRYIADRVDVIVAKYG
                     EGNFNSHGRGMFRGINCVSGDLAGQITRRCFQKGLIIETSGADDHVVKFLCPLIITDEE
                     LEKGIDILEQAIKEVCDKADTIPEGKDFFEGDYSVSKEVSKAGH"
     aSDomain        2333..3343
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ectB_Aminotran_3.1"
                     /evalue="6.80E-79"
                     /label="ectB_Aminotran_3.1"
                     /locus_tag="ectB"
                     /protein_end="358"
                     /protein_start="21"
                     /score="257.1"
                     /tool="antismash"
                     /translation="FDRAQGAKIYDHEGNEYLDFLAGAGSLNYGHNHPVFKEKLLEYIQ
                     NDGITQGLDLHTRAKGEFLESFNEHILKPRNLDYIVMFTGPTGTNAVEAALKIARKKTG
                     RENIISFTNGWHGQTLGSLSVTGNSTHRGGAGIALHGSTRIPYDGYLGDDFDTTNLLDK
                     MLSDSSSGVDKPAAVIVETVQGEGGINAASMTWLRSLSEICKRHDVLLIIDDIQAGCGR
                     TGTFFSFEEAGIYPDIVTLSKSLSGYGLPLAIVLMKPEIDQWSPGEHNGTFRGNNHAFV
                     TAKAAIDHFWKDDSFAKDVQRKGRYIADRVDVIVAKYGEGNFNSHGRGMFRGINCV"
     gene            3652..4056
                     /gene="ectC"
     CDS             3652..4056
                     /codon_start=1
                     /function="catalyzes the cyclic condensation of
                     gamma-N-acetyl-diaminobutyric acid into ectoine"
                     /gene="ectC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /product="ectoine synthase"
                     /protein_id="AAY96772.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.7e-47, bitscore:
                     149.4, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRQLQDAQQTNRRIVSPDGNWESTRLLLKDDGMGYSFHITTIF
                     AGADFRMHYQNHLESVYCISGEGEVETLDDGKKYPITPGTLYNLDQHDRHILRAFKELQ
                     LACVFNPPLNGKEVHNAEGAYELEAETVSD"
     gene            4117..5559
                     /gene="ask"
     CDS             4117..5559
                     /codon_start=1
                     /gene="ask"
                     /product="aspartokinase"
                     /protein_id="AAY96773.1"
                     /transl_table=11
                     /translation="MTFHTVEKIGGTSMSDYVAVRDNIILKPVHREDLYQRIFVVSAYG
                     GITDMLLEHKKNGQAGIYGQFANSVNDDGWQASLVALKQAMFAINANIFDTADAIKKAD
                     TFIGERLDDAERCLADLQHLCRHGHFSLDAHLSTVREMLASLGEAHSAWNTVQLLQQDG
                     INAVFVDLTGWQTDKHMTLDERIKNAFAEIDLRQQLPIATGYAHSIDGLMSTFDRGYSE
                     MTFSRIAVLTKADEAIIHKEFHLSSADPRLVGEDKAVPIGRTNYDVADQLANLGMEAIH
                     PKAAKGLRQNDIALRVRNTFEPDHAGTLITGDYVSSKPCVEIIAGCKGVYAFEVFDQNM
                     AGEIHNYDREILGQIRRFRAHIVSKDINANTITHYLSASLKTIRRIRDALQEIYPDAEI
                     NQQKVSIVSAIGSDMKIPGILAKTVSALAEKQISVLAMHQSMRQVDMQFVIDEDAYTDA
                     MKSLHCHLVEVHDHGIAICLAS"
     gene            5920..6879
                     /gene="phy"
     CDS             5920..6879
                     /codon_start=1
                     /gene="phy"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /product="putative ectoine hydroxylase"
                     /protein_id="AAY96769.2"
                     /sec_met_domain="PhyH (E-value: 1.2e-36, bitscore: 115.7,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MITENAAQSEQSEDFYQSRNGSKPKIIPRVDPVVYAQTANPGLIA
                     EDLQARYEQQGFLVIDNVFNEREVDCFKQELKRLNDDEKIKASAEAITELSSDELRSLF
                     KIHEVSPVFKRLAADNRLAGLAQHLLNDRVYIHQSRLNYKPGFRGKEFYWHSDFETWHV
                     EDGMPRMRALSMSIILTENDQHNGPLMLVPGSHKKFVVCEEETPENHYSVSLKKQEYGI
                     PSDECLASLVADGGIVSANGKPGSVLIFDSNVMHGSNSNITPWPRSNLFFVYNAINNRV
                     TWPFCGLLPRPEYLCSRKNIRVIEPRPFIAAADQLIYA"
ORIGIN
        1 ggcaactagg ctaaatatgc ctttgtgctg ttacccaagc tccagctctc atcgtgtaat
       61 tattcacacc gcctatcgtt ctcacgctcc ggcgtgggaa tgaagaccga gacgctctag
      121 cgtctcgtac cgctggagcg gtactcaggc attcccacgc agagcgtggg aacgataatt
      181 gccgggggac tgaatagtta cctcatcgtt atacataagt caaaaattac ccttttgcaa
      241 tcttaaccaa tgaggcctcg ataccatttt ttatcgccca acgttcccga cttcgaagcc
      301 actgatgtcc aatatccgca gatttatcaa acagcaccgc ttccaagtat acggcgacct
      361 ctgtttagca agtttaccga tatttgtgta tagcggcgag cgatccggct tgggtaatct
      421 attcaggaag ctctagcttc ccgacaatca aaaaagatcc tgattagcaa gtttcttcag
      481 ctaaagcccg aaaaccagca tatccttagc aggacggggt ttgcagcccc gtcctaaacg
      541 ttttgacttt ggccgaagtc agccgaaatg tttagggcaa accgaaacgt tggggatggg
      601 ttaaataacc cgtcccgcag aagtggctac gactggccac tgctcggaca attggcttca
      661 gcaagttgaa gcatcttgct aatcaggaaa aagattgaac gagcgagtcg gggttgattg
      721 agaatgtgcg gaggttatct ccggttttct ggaagctgga gcttccgggg tgtttttccc
      781 aagctggagc ttgggaaaaa gcgtgatgcg ggttgcagct gagctgaatt attagagctt
      841 aaaccggaaa atcaaacgct tctttagcga cagggatgtt ctgcgcgtcc atcatctgag
      901 cgacacgttg cagtgatgca ataatcatca attgttccca ttcctgtagt ttactgaatt
      961 ctcgcgtaaa gcgatcctgc aaagggatag gggcgtttat tagcatttcc gtggccgcct
     1021 ccgtcatata gacatggact tttcgtttat cagtctggga gcgctgccgg aacaccaatt
     1081 gacgtttttc caggcgatcc agaattgttg tcacagtcgc ttggctgaga ctcatgtcct
     1141 gagctagctc accaatggtc agttgacctt tggcgcgcag tgtctgcaac aaaagaatct
     1201 gcggtgcggt caggctagtg gtttttgcca gatattttga gtgtaaatcg gtggcgcgaa
     1261 tcactctacg taacgcgacc agtacttctt caatttgatt cagcaaatgt tccttttcaa
     1321 ccggattcaa gtcgagcgta tccagcgaat gaggggcgtg cggttgtggg ttttgcatca
     1381 ttgatcacca atcattgaaa tagaggccgt cgcgttacaa agccattatt gtataccctc
     1441 gaagaacaaa attcggtgct ccgcccggcg catcctaggg cgctaccgaa atttgaagtt
     1501 cgaaaggcat aatttgatat gacatctaat taatctaagg caaaagtacg cggcctggac
     1561 atttaaatta tttagtgtac tatatattat gtttttcaat gaagccagaa tattcgtgct
     1621 gatggccata aaaaatcaaa ttcgagcccg gcttattcag ttaaagccga gttaattgca
     1681 cagaaatcta aataatagag ttatttatat gttgcctgat aaaactgcct tgccgattat
     1741 tacacttagc cagcctactg ccgaagtcgg agcgcaggtg catcggctga ttagcaaatg
     1801 cccgcccctg gatcccaatt cgatgtactg caatttgctg cagagcagcc atttttctga
     1861 aacggcggtg gcggccaaaa ttggcgatga gttggttggt tttgtctcgg gttatcgtat
     1921 tccgcaacgt ccggatacgc tgtttgtctg gcaggtggct gtcggcgaaa aagcccgtgg
     1981 ccaagggctt gccacgcgga tgctgaaagc catactggcg cggccggtca atcaggatat
     2041 taaccgaatc gagacgacca ttactccgaa taataaggcc tcatgggctc tgtttgaggg
     2101 gctggcaaaa aaactggaca ctcaaattgg cagtgctgtg atgtttgata agacacgcca
     2161 ttttgcagat caacatgaaa ccgaaatgct ggtgaaggtt gggccgttca aagcggttca
     2221 ggcttaattc actacactat tatcccatct tgctcaggag agaattagaa tgaaaatctt
     2281 cgaagaaatt gaatcggaag tccgttcgta tgcccgcgct tttccgcgcg tgtttgatcg
     2341 tgcccaaggc gcaaaaatct atgatcatga aggcaacgaa tatctggact ttctcgctgg
     2401 tgcaggttcc ctgaattacg gtcacaatca cccggtattt aaggaaaagc tgcttgagta
     2461 tatccagaac gatggcatca cgcagggctt ggacttgcac acccgtgcca agggtgagtt
     2521 tttggaaagc tttaatgagc acatccttaa gccgcggaac ttggactaca ttgtgatgtt
     2581 taccggtccc accggcacca acgcggttga agcggcactg aagatcgccc gcaaaaaaac
     2641 cggtcgtgaa aacattatct cgtttaccaa tggctggcat ggtcagacgc tgggatcgct
     2701 gtcggttacc ggtaattcca ctcaccgtgg cggcgccggc attgctttgc atggttcaac
     2761 ccgtattcct tatgacggct atttaggtga tgattttgat accacaaatt tgctcgataa
     2821 aatgctgtcg gattccagca gtggtgtcga taagccggct gccgtcatcg ttgaaacggt
     2881 tcagggcgaa ggcggtatca atgccgcgtc gatgacttgg ttaagaagcc tgtcggaaat
     2941 ctgcaaacgg catgatgtgt tgctgatcat tgatgatatc caggccggtt gcggccgcac
     3001 cggtaccttc ttcagttttg aagaagccgg aatttatcct gatattgtaa ccttgtcgaa
     3061 atcgttgagc ggttatggtt tgccgttagc cattgttctg atgaaaccgg aaatcgatca
     3121 atggtcacca ggcgagcata atggcacctt ccgaggtaac aatcatgcct ttgtcactgc
     3181 caaggcggct attgatcact tctggaaaga cgacagcttt gccaaggatg tccagcgcaa
     3241 aggtcggtac attgccgatc gtgtagatgt tattgtcgcc aagtatggcg agggtaattt
     3301 caactcgcat gggcgaggca tgttccgggg cattaactgt gtcagcggtg atttggccgg
     3361 tcagattacc cgcagatgtt ttcagaaagg tctgattatt gaaaccagtg gtgcggacga
     3421 tcatgtcgtg aagttcttgt gccctctgat cattacagat gaggagctgg aaaaaggcat
     3481 cgatattctg gagcaagcca ttaaagaggt ctgtgataaa gcagacacaa tccctgaggg
     3541 aaaagacttt tttgaaggcg actactcggt cagtaaagag gtcagcaaag cggggcacta
     3601 aagcccgctg agccaaccag cctattcgag aatctactga agagaactaa aatgatcgta
     3661 agacagttac aagacgcaca gcagactaac agacgcatcg tttcacctga cggcaactgg
     3721 gaaagtaccc gcttgctgct gaaggatgac ggtatgggct attcctttca tatcaccacc
     3781 attttcgccg gtgccgattt tcgcatgcat taccagaatc accttgagtc ggtgtattgc
     3841 atcagtggtg aaggcgaagt ggaaacgctg gacgatggta aaaagtaccc tattacgcca
     3901 ggcacactct ataacctgga tcagcatgat cggcatattc tgcgtgcttt caaggagctg
     3961 caactcgctt gtgtctttaa cccgcctctg aacggtaagg aagtacacaa cgctgaaggg
     4021 gcctatgaac tggaagccga aacagtcagt gactgatgtt tcactcatgg cagggcggtt
     4081 gaagccctcg gcatccacga agtaagagag gacgttatga cttttcacac cgttgaaaaa
     4141 atcggcggca catccatgag cgattatgtt gcggtgcgcg acaatatcat tctcaagccg
     4201 gttcatcgcg aggaccttta tcagcgtatt tttgtggtct ccgcctatgg tggtatcacc
     4261 gatatgttgc tagaacacaa aaaaaacggt caggccggca tttatggtca atttgccaac
     4321 agtgtaaacg atgacggctg gcaggcttcg ctggtagctc tcaagcaggc aatgtttgcg
     4381 attaatgcca acatttttga tactgctgac gccataaaaa aagcagatac gttcattggc
     4441 gaacggcttg atgatgcaga gcgctgtctg gccgatttgc agcatctttg tcggcacggt
     4501 catttttcgc tggatgcgca tctttctacc gttcgggaga tgctggccag tctcggtgag
     4561 gcgcacagcg cgtggaacac tgtccagttg ttgcaacaag acggtattaa cgcagtcttt
     4621 gtcgatctga ccggttggca gaccgataaa cacatgacgc tcgacgaacg cattaaaaat
     4681 gcctttgctg agatcgatct gaggcaacag ttaccaattg ccacaggcta tgcgcacagt
     4741 atcgacgggt tgatgtcaac gtttgaccgg ggctacagtg aaatgacctt cagccggatt
     4801 gcggtactga ccaaggcgga tgaggccatt attcataagg agtttcatct cagcagcgct
     4861 gatccgcgtc tggtaggtga agacaaggcc gtgccgattg gccgtaccaa ttacgatgtg
     4921 gccgaccagc ttgcgaacct gggcatggaa gcgattcatc ccaaggcggc aaaaggcttg
     4981 cgtcagaacg atatagcgct acgcgtcaga aacacctttg agccggatca tgccgggacg
     5041 ctgattaccg gcgattatgt cagtagcaag ccttgcgtag agattattgc cgggtgtaaa
     5101 ggcgtctatg ctttcgaggt gttcgatcaa aacatggccg gcgagattca caattacgat
     5161 cgggaaatcc tggggcaaat tcgccgcttc agagcgcata ttgtttccaa agatattaat
     5221 gccaatacca ttactcatta tttgtctgcc agtctgaaaa caattcgccg catccgcgat
     5281 gccttgcaag aaatctatcc ggatgccgaa atcaatcagc aaaaagtttc gattgtttcc
     5341 gccatcggca gtgacatgaa aattcccggt attctggcca aaactgtctc ggcgctggca
     5401 gagaagcaga tcagcgtgct tgcaatgcac cagtcaatgc gtcaagtcga tatgcagttt
     5461 gtgattgatg aagatgccta cactgatgcg atgaaaagtt tgcattgtca tctggtggag
     5521 gtccatgatc acggcattgc aatatgcctc gcgtcctgat tgtactgatg tttcttaccc
     5581 ctaaatacgg ggaaatttct caaactggga attgctgcca aagaaatgaa aatgccttgc
     5641 gtcttcagtc ttgcgctgaa cgacaaggaa gcgcataagg cttaaagcgt ttaccactcg
     5701 accgctgaag cggtagcgga ttaaggcgag tcgcaagtca attttcgtcc aaggttaggt
     5761 tgttcatggc aagtcagtcg agcaatgaat gacttaaccg tctatttttc aataaactga
     5821 agatgtacgg gtaagccctg cgtaagttgg gaatggccga tgatcgaggg ctatctgttg
     5881 tcgcgaagtc tttaatcaaa aaaatgggtt taatattcaa tgattaccga gaatgccgca
     5941 cagtccgaac aaagtgaaga tttttatcaa tcacgtaacg gtagtaagcc gaaaataatt
     6001 ccgcgcgtag acccggtagt ttatgcgcaa acagctaatc caggtctcat tgcagaggac
     6061 ttgcaagcac gttatgagca acaaggtttt cttgttattg ataatgtttt taatgagagg
     6121 gaggtcgact gtttcaagca agagctcaaa cgcttgaacg acgatgaaaa gataaaagcc
     6181 tcggcggaag cgataactga attatccagc gacgaactcc gttcactatt taaaattcat
     6241 gaagtcagtc cggtttttaa aaggttagct gccgataatc gattagcggg actggctcaa
     6301 catcttttga acgaccgggt ttatattcat cagtcgcgct taaactataa gccgggtttt
     6361 cgcggcaagg aattttactg gcattcggac tttgaaactt ggcatgtaga agacggtatg
     6421 cctagaatgc gtgcgctcag catgtccatt attcttaccg aaaacgatca gcataacggg
     6481 cctttgatgt tggttcccgg atcgcataaa aaatttgtcg tttgcgaaga ggaaacgccg
     6541 gaaaatcatt attcggtctc gttgaaaaag caggagtacg gcatacccag cgatgaatgc
     6601 ttggctagct tggttgccga tggcggcatc gtatcggcca atggaaaacc cggcagtgtc
     6661 ttgattttcg acagtaatgt catgcacggt tcgaatagta atatcactcc atggcctcgc
     6721 tcgaatctct ttttcgtcta taacgcgatc aataatcgag taacatggcc gttttgcggt
     6781 ttattgccgc gtcctgaata tctttgcagt cgcaagaata tacgagttat cgaaccgcgg
     6841 ccttttatcg cggccgccga tcaattgata tatgcttaga atgttaataa tgttgatcgt
     6901 gctggcg
//
