LOCUS       BGC0000856              4347 bp    DNA     linear   BCT 30-DEC-2011
DEFINITION  Methylophaga thalassica strain MT ectoine biosynthesis gene cluster,
            complete sequence; and aspartokinase (ask) gene, partial cds.
ACCESSION   BGC0000856
VERSION     BGC0000856
KEYWORDS    .
SOURCE      Methylophaga thalassica
  ORGANISM  Methylophaga thalassica
            Bacteria; Proteobacteria; Gammaproteobacteria; Thiotrichales;
            Piscirickettsiaceae; Methylophaga.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-07 20:50:18
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4347
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..4347
                     /db_xref="taxon:40223"
                     /mol_type="genomic DNA"
                     /organism="Methylophaga thalassica"
                     /strain="MT"
     protocluster    1..4347
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[2496:2901]"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      2497..2901
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     cand_cluster    1..4347
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..4347
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000856"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..4347
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            518..1027
                     /gene="ectA"
     CDS             518..1027
                     /codon_start=1
                     /gene="ectA"
                     /note="EctA"
                     /product="L-2,4-diaminobutyric acid acetyltransferase"
                     /protein_id="ABY40739.1"
                     /transl_table=11
                     /translation="MTNNKPEKASITLKEPTAEDGASVFDLISACPPLDTNSMYCNLLQ
                     SSHFSQTSVAAEMDGNLVGFISGYVLPKQPDTLFIWQVAVGEKARGQGLASRMLRHILE
                     RDICKPVKYIETTITPDNRASWALFESLANKLNTQLNRSVMFDRQQHFAGQHETEMLVK
                     VGPFEL"
     misc_feature    518..2901
                     /note="ectoine biosynthesis gene cluster"
     gene            1088..2425
                     /gene="ectB"
     CDS             1088..2425
                     /NRPS_PKS="Domain: Aminotran_3 (22-358). E-value: 3.2e-74.
                     Score: 241.8. Matches aSDomain:
                     nrpspksdomains_ectB_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013:aminotransferase class-III (Score: 361.6;
                     E-value: 8.6e-110)"
                     /gene_kind="biosynthetic-additional"
                     /note="EctB"
                     /product="L-2,4-diaminobutyrate aminotransferase"
                     /protein_id="ABY40740.1"
                     /sec_met_domain="Aminotran_3 (E-value: 1.5e-79, bitscore:
                     255.6, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKIFDEIESEVRSYARSFPRMFNRAKDEFIYDEEGNAYLDFLAGA
                     GSLNYGHNNDLFKEKLLEYINNDGITQGLDLHTRAKGEFLESFNNNILKPRDLDYIVMF
                     TGPTGTNAVEAALKTARKYTGRENIVSFTNGWHGQTLGSLAITGNGHHRGGAGVSLSGA
                     TRMPYDGYMGDDVDTTNYLDKVLSDSSSGVDKPAAVIVETVQGEGGINACSTTWLKSLS
                     EVCKKHDVLLIIDDIQAGCGRTGTFFSFEEAGIYPDIVTVSKSLSGYGLPFAIVLMKPE
                     LDQWKPGEHNGTFRGNNPAFITAKAALDNYWKDDKFAKEVQAKGEYIANRVDKIVELYG
                     EGNFNSHGRGMFRGINCVSGEIAGKITHNAFQKGLIIETSGADDHVVKFLCPLTITQEN
                     LEKGIDILEQSIKEVCAKADNIPEEKDYFEGDYSVSEEVEKMSRQS"
     aSDomain        1154..2161
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ectB_Aminotran_3.1"
                     /evalue="3.20E-74"
                     /label="ectB_Aminotran_3.1"
                     /locus_tag="ectB"
                     /protein_end="358"
                     /protein_start="22"
                     /score="241.8"
                     /tool="antismash"
                     /translation="NRAKDEFIYDEEGNAYLDFLAGAGSLNYGHNNDLFKEKLLEYINN
                     DGITQGLDLHTRAKGEFLESFNNNILKPRDLDYIVMFTGPTGTNAVEAALKTARKYTGR
                     ENIVSFTNGWHGQTLGSLAITGNGHHRGGAGVSLSGATRMPYDGYMGDDVDTTNYLDKV
                     LSDSSSGVDKPAAVIVETVQGEGGINACSTTWLKSLSEVCKKHDVLLIIDDIQAGCGRT
                     GTFFSFEEAGIYPDIVTVSKSLSGYGLPFAIVLMKPELDQWKPGEHNGTFRGNNPAFIT
                     AKAALDNYWKDDKFAKEVQAKGEYIANRVDKIVELYGEGNFNSHGRGMFRGINCV"
     gene            2497..2901
                     /gene="ectC"
     CDS             2497..2901
                     /codon_start=1
                     /gene="ectC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /note="EctC"
                     /product="ectoine synthase"
                     /protein_id="ABY40741.1"
                     /sec_met_domain="ectoine_synt (E-value: 3.5e-48, bitscore:
                     151.0, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLVRQLQEAEKTDRKIVDPNGNWDSTRLLLKDDNMGFSFHITTIF
                     EGADFRMHYQNHLESVYCISGEGEVETLDDGKKYAITPGTLYNLDKHDKHMLRAFKEMK
                     LACVFNPPLHGTEVHNKDGAYELEAETIKD"
     gene            3034..>4347
                     /gene="ask"
     CDS             3034..>4347
                     /codon_start=1
                     /gene="ask"
                     /note="Ask"
                     /product="aspartokinase"
                     /protein_id="ABY40742.1"
                     /transl_table=11
                     /translation="MSFHTVEKIGGTSMSDYVAVRDNIILKPVHHDSLYNRIFVVSAYG
                     GITDLLLEHKKTGQPGVFGLFANGLHDDSWKQALADLKQKIFTINESFYKKPDNLERAN
                     QFIGERLDDAERCLADLQRLCQHGHFELDMHLETVREMLASIGEAHSAWNMTQLLQRDG
                     IDAIFVDLTGWQTDKHLPLDERIKIAFQDVDLKKQLPIVTGYAHSDDGLMSTFDRGYSE
                     MSFSRIAVLTEANEAIIHKEFHLSSADPRLVGEDNAVPIGRTNYDVADQLANLGMEAIH
                     PKAAKGLRRNEIALRVRNTFEPEHAGTLITGDYISATPCVEIIAGCKGVYAFELFDQDM
                     AGNIDVYDREILTMIDRFKAHIISKDINANTITHYLSANLKTIKRIRNALQERFVDAEI
                     NQQKVAIVSAIGSDMKIPGILAKTVGALAKENISVLAMH"
ORIGIN
        1 ggtaccatat gttgtaaatc cagcaaggta attttcacga cttcatcaac agtaatacca
       61 actaagccat tatctgattt gataataatg atgtgacggt gctcatctgc ctcatcaatt
      121 cctaataatt tcctactact cacaatgctg actatctcgc ctcttatgtt cagtactcct
      181 tctatatatg aaggggcacc gggtacaggg accgctgcca tatagtcgtg aacttccctt
      241 atcttactta ctgcgtgtgc ataggtttca tcttcaatca gaaaacaaag ccattgtttg
      301 ttgtcatgta actctaatgt catctattga atatacgtta ttaatctcgg ggtggacaat
      361 ataattattt agtgtactat atatattaaa tcaataacag cccgaattat ttatacaact
      421 tgctgtttat tatgaattat aagatgtctt taactattta caggcttttt ataagtaaaa
      481 aaacaataga gatataaaca atagagctat ttacataatg actaacaata aacctgaaaa
      541 agcttcaatt acattaaaag agccaacagc tgaagacggt gcttccgtct ttgatttaat
      601 ttcagcctgt cctcctcttg ataccaattc catgtactgc aatttgctgc aaagcagtca
      661 tttttctcag acttcagtgg ctgcagagat ggatggaaac ctcgttggat ttatttctgg
      721 atacgttctg cctaaacaac ctgacacctt attcatatgg caagtcgcgg ttggagaaaa
      781 agcaagagga caagggcttg caagccgcat gctccgtcac atcttagaac gtgatatctg
      841 caaacccgtg aaatatatcg aaaccactat cactcccgat aatcgtgcgt catgggcctt
      901 atttgagagt ctggcgaata aattgaatac acaactaaac cgttcagtga tgtttgatcg
      961 ccaacaacat tttgctggcc aacatgaaac ggaaatgttg gttaaagtcg gtccttttga
     1021 gctgtaagtc attaatttct gcacatctgg acacgaataa aaaatactac taggagaaaa
     1081 aaaacctatg aaaatctttg atgaaattga atcagaagtt cgttcttacg ctcgttcttt
     1141 tcctcgcatg tttaaccgtg ccaaagacga gtttatctat gatgaagaag ggaatgcgta
     1201 ccttgacttt ctggcagggg ctggttcatt gaattatggt cacaacaatg acctgttcaa
     1261 agagaaactt ctcgaataca ttaataacga tgggattacc caaggcctgg atttacacac
     1321 tcgtgctaaa ggtgaattct tagaaagttt taataacaac attcttaagc ctcgtgatct
     1381 tgattacatc gtgatgttta ctggcccaac tggtaccaat gccgttgaag ccgcgttaaa
     1441 aacagcacgt aaatatactg gcagagaaaa tattgtttca tttactaatg gttggcatgg
     1501 tcagacatta ggttcactgg ctattactgg taacggtcat catcgtggtg gtgctggcgt
     1561 tagcctgagt ggtgcaacac gtatgcctta tgatggttat atgggtgatg atgtagatac
     1621 caccaactat cttgataaag tactttcaga ttcaagcagt ggtgtagaca agcctgcagc
     1681 tgtaattgtt gaaacagttc agggtgaagg cggtattaac gcttgctcaa caacctggtt
     1741 aaagagcctg tcagaagtat gtaaaaaaca tgatgtgctg ttgattatcg atgatatcca
     1801 ggcgggttgt ggtcgtactg gtaccttctt cagcttcgaa gaagcgggta tctaccctga
     1861 catcgttacc gtatcaaaat cattaagtgg ctacggtctg ccatttgcta tcgtgttgat
     1921 gaaacctgag ttggatcaat ggaaaccggg tgaacataac ggtacattcc gtggtaacaa
     1981 tcctgcattt atcactgcta aggcagcgct ggataactat tggaaagacg acaaatttgc
     2041 taaagaagta caggcaaaag gtgagtacat tgccaatcgc gtagacaaaa ttgttgagct
     2101 atatggtgaa ggcaacttta actcacatgg tcgtggcatg ttccgtggta ttaactgtgt
     2161 gagtggtgaa attgctggaa aaatcaccca taacgctttc caaaaaggat tgatcatcga
     2221 gacaagtggt gccgatgacc acgtggttaa attcttatgt ccactgacta tcactcaaga
     2281 aaacctggaa aaaggtatcg acattcttga gcaatcaatt aaagaagtct gtgctaaagc
     2341 tgacaatatt ccagaagaaa aagattactt cgaaggtgat tactcagtca gtgaagaagt
     2401 cgagaaaatg tctcgtcagt cttaattgct tataagaaaa cgggtggttc gccgcccgtt
     2461 tctattccaa ataataatca aataagagga aatgaaatgc ttgttagaca acttcaggaa
     2521 gcagaaaaaa ccgaccgtaa aattgttgat cctaatggta attgggatag cacccgcctg
     2581 ttattaaaag atgacaatat gggattctct ttccatatta cgactatctt tgaaggcgct
     2641 gatttcagaa tgcattatca gaatcacctt gagtcggtgt attgcatcag tggtgaaggt
     2701 gaagttgaaa cattggatga tgggaaaaaa tatgccatca cgccaggtac cttatacaac
     2761 cttgataaac acgataaaca catgctgcgt gcatttaaag aaatgaaatt ggcatgtgtg
     2821 tttaaccctc cactacatgg cacggaagtt cataacaaag atggtgctta tgaattagaa
     2881 gcagagacta ttaaagacta acttgctcag gcatcgatca gggcatgatc ggtgcctacc
     2941 tcaagctata acagtcaatg aatatcttgt tcactcgtcg ttttgacggt gaacaagata
     3001 ctaatccaaa aaggatgaaa tagagagaat acaatgagtt ttcatacggt agaaaaaatc
     3061 ggcggcactt ctatgagcga ctatgttgcg gtgcgcgata acattattct gaaaccggtt
     3121 catcacgact cgctctacaa tcgaatattt gttgtttcag cttatggcgg tatcaccgac
     3181 ctgttgctgg aacataaaaa gaccggacaa ccaggtgtgt tcggcttatt tgcaaacgga
     3241 ctgcatgatg atagctggaa acaagcttta gcagatttaa agcaaaagat tttcactatc
     3301 aatgaaagtt tttataaaaa acctgacaat cttgaacgtg ctaatcagtt cataggtgaa
     3361 cgtcttgatg atgctgaacg ctgtttggct gatctacaaa gactttgcca gcatggccac
     3421 tttgagctgg atatgcatct tgaaaccgtg cgggaaatgc tcgccagcat tggtgaagct
     3481 cacagtgcat ggaatatgac gcagttgctt cagcgtgatg gtattgatgc tatttttgtt
     3541 gatttaaccg gctggcaaac agacaagcac ttacctttag acgaacgtat caaaatcgct
     3601 tttcaggatg tcgacctcaa aaagcaattg cctatcgtca ccggttatgc acatagcgat
     3661 gatggtctaa tgtcaacatt tgaccgtggt tacagtgaaa tgagcttcag ccgtattgct
     3721 gtgcttacag aagcaaacga agccatcatt cataaagaat tccatctcag cagtgctgat
     3781 ccccgattag tgggtgaaga taatgccgta cctattggcc gaacaaatta cgatgttgct
     3841 gatcaattag ccaatttagg tatggaagcc attcatccta aagcagccaa aggcttacgc
     3901 agaaacgaaa ttgctctgcg tgtacgcaat acttttgaac ctgaacatgc cggcacatta
     3961 attacgggtg actatataag tgcaacaccc tgtgttgaga tcattgccgg ttgtaaaggg
     4021 gtgtatgcgt ttgaactctt tgatcaagat atggcaggta atattgacgt ttatgatcgt
     4081 gaaatcctga cgatgataga cagatttaaa gcacatatta tctctaaaga tatcaatgcc
     4141 aacacgatta cccactatct ctctgccaat ctcaaaacaa taaaacgtat ccgtaatgcc
     4201 ttacaagaac gctttgttga tgctgaaatt aatcaacaaa aagtggcgat tgtgtctgcc
     4261 atcggtagtg atatgaagat cccgggtatt ttagcaaaaa ccgttggcgc gcttgcgaaa
     4321 gaaaacatca gtgttttagc catgcat
//
