LOCUS       BGC0000857              4871 bp    DNA     linear   BCT 31-DEC-2009
DEFINITION  Methylophaga alcalica strain M8 putative transcriptional regulator
            gene, complete cds; ectoine biosynthesis gene cluster, complete
            sequence; and aspartokinase (ask) gene, partial cds.
ACCESSION   BGC0000857
VERSION     BGC0000857
KEYWORDS    .
SOURCE      Methylophaga alcalica
  ORGANISM  Methylophaga alcalica
            Bacteria; Proteobacteria; Gammaproteobacteria; Thiotrichales;
            Piscirickettsiaceae; Methylophaga.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-07 20:50:27
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..4871
                     /db_xref="taxon:182774"
                     /mol_type="genomic DNA"
                     /organism="Methylophaga alcalica"
                     /strain="M8"
     protocluster    1..4871
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[3082:3487]"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      3083..3487
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     cand_cluster    1..4871
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..4871
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000857"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..4871
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /subregion_numbers="1"
                     /tool="antismash"
     CDS             complement(301..867)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1135:MarR family
                     transcriptional regulator (Score: 80; E-value: 2.1e-24)"
                     /gene_kind="regulatory"
                     /note="MarR family protein"
                     /product="putative transcriptional regulator"
                     /protein_id="ABY40743.1"
                     /transl_table=11
                     /translation="MEKSDNRNTTSIDTLGLKPTQREHLLDQIEEVLVALRRVIRATDL
                     HSKYLVKTTSLTAPQILLLQTLRDKGQLTIGELAQDMSLSQATVTTILDRLEKRQLVYR
                     QRSQTDKRKVHAYMTDEAMTMLKNAPIPLQDRFTREFSQLQEWEQTMIISSLKRVAQMM
                     DAKPLTSAAEVSQQINEDKTAAESR"
     gene            1136..1654
                     /gene="ectA"
     CDS             1136..1654
                     /codon_start=1
                     /gene="ectA"
                     /note="EctA"
                     /product="L-2,4-diaminobutyric acid acetyltransferase"
                     /protein_id="ABY40744.1"
                     /transl_table=11
                     /translation="MQAVKQNTSPITLIQPTAEIGAQVYQLIANCPPLDTNSMYCNLLQ
                     SSHFAETAVAAMLDEEVVGFISGYRIPQRPDSLFVWQVAVGEKARGQGLATQMLKAILS
                     RPENQDIKRIETTITPDNRASWALFEGLARKLDTEISSSVMFDKTTHFADQHASEMLVK
                     VGPFKSQKL"
     misc_feature    1136..3487
                     /note="ectoine biosynthesis gene cluster"
     gene            1709..3037
                     /gene="ectB"
     CDS             1709..3037
                     /NRPS_PKS="Domain: Aminotran_3 (21-358). E-value: 3e-80.
                     Score: 261.6. Matches aSDomain:
                     nrpspksdomains_ectB_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013:aminotransferase class-III (Score: 383.9;
                     E-value: 1.5e-116)"
                     /gene_kind="biosynthetic-additional"
                     /note="EctB"
                     /product="L-2,4-diaminobutyrate aminotransferase"
                     /protein_id="ABY40745.1"
                     /sec_met_domain="Aminotran_3 (E-value: 1.2e-85, bitscore:
                     275.9, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKIFEEIESEVRSYARAFPRVFDRAQGALIYDREGNEYLDFLAGA
                     GSLNYGHNHPIFKEKLIEYIQADGITQGLDLHTRAKGEFLESFNEHILKPRNLDYVVMF
                     TGPTGTNAVEAALKIARKKTGRENVISFTNGWHGQTLGALSVTGNSTHRGGAGIALHGS
                     TRIPYDGYLGDDFDTTNLLDKMLSDSSSGVDKPAAVIVETVQGEGGINAASMTWLKSLS
                     EICKRHDVLLIIDDIQAGCGRTGTFFSFEEAGIYPDIVTLSKSLSGYGLPFAIVLMKPE
                     VDQWSPGEHNGTFRGNNHAFVTAKAALDHFWKDDKFAKEVQRKGDYIAKRVDSIVAEYG
                     EGNFNSHGRGMFRGINCVNGELAGQITRKCFQKGLIIETSGADDHVVKFLCPLIITDEQ
                     LTKGIDILEEAIKEVCAKADSIPEEKDFFEGDYSVSDEVNKAS"
     aSDomain        1772..2782
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ectB_Aminotran_3.1"
                     /evalue="3.00E-80"
                     /label="ectB_Aminotran_3.1"
                     /locus_tag="ectB"
                     /protein_end="358"
                     /protein_start="21"
                     /score="261.6"
                     /tool="antismash"
                     /translation="FDRAQGALIYDREGNEYLDFLAGAGSLNYGHNHPIFKEKLIEYIQ
                     ADGITQGLDLHTRAKGEFLESFNEHILKPRNLDYVVMFTGPTGTNAVEAALKIARKKTG
                     RENVISFTNGWHGQTLGALSVTGNSTHRGGAGIALHGSTRIPYDGYLGDDFDTTNLLDK
                     MLSDSSSGVDKPAAVIVETVQGEGGINAASMTWLKSLSEICKRHDVLLIIDDIQAGCGR
                     TGTFFSFEEAGIYPDIVTLSKSLSGYGLPFAIVLMKPEVDQWSPGEHNGTFRGNNHAFV
                     TAKAALDHFWKDDKFAKEVQRKGDYIAKRVDSIVAEYGEGNFNSHGRGMFRGINCV"
     gene            3083..3487
                     /gene="ectC"
     CDS             3083..3487
                     /codon_start=1
                     /gene="ectC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /note="EctC"
                     /product="ectoine synthase"
                     /protein_id="ABY40746.1"
                     /sec_met_domain="ectoine_synt (E-value: 3.7e-48, bitscore:
                     151.2, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRQLQEAQKSSRRIVSPDGNWESTRMLLKDDNMGYSFHITTIF
                     AGADFRMHYQNHLESVYCISGEGEVESLDDGKIYPITPGTLYNLDQHDRHILRAFKELH
                     LACVFNPPLNGKEVHNKEGAYELDAESVTE"
     gene            3558..>4871
                     /gene="ask"
     CDS             3558..>4871
                     /codon_start=1
                     /gene="ask"
                     /note="Ask"
                     /product="aspartokinase"
                     /protein_id="ABY40747.1"
                     /transl_table=11
                     /translation="MDFHTVEKIGGTSMSDYVAVRDNVILKPVHSTSLYQRIFVVSAYG
                     GITNLLLEHKKNGQAGIYGQFANSVNDDGWQASLEQLKQEMLAINATLFDSAETIAKAD
                     AFISERLDDTERCLADLQRLCQHGHFSLDGHLATVREMLASIGEAHSAWNTVQLLQQDD
                     INAVFVDLTGWQTDKHMTLDERIETAFAKIDLNTQLPIVTGYAHSIDGLMSTFDRGYSE
                     MTFSRLAVLTNANEAIIHKEFHLSSADPRLVGEDNAVPIGRTNYDVADQLANLGMEAIH
                     PKAAKGLRKNEIPLRVRNTFEPEHAGTLITGDYVSSKPCVEIIAGCKGVYAFEVFDQDM
                     AGEIDSYDKEILAQIRRFKAHIVSKDINANTITHYLTASLKKIRRIRDALQERFPDAEI
                     NQQKVAIVSAIGSDMKIPGILAKTVAALAEKNISVLAMH"
ORIGIN
        1 ggtaccggtt attcgtcact gagttatttg aagcactttc cggttgatac gctgaaaatt
       61 gataaatcgt ttgtggctga aattgtcttt aactctgcag atgcggccat tgcacggacc
      121 attatccagc tggcggaaaa tttaggatta aacacggtgg cagaaggagt tgaaacgctg
      181 gatcaagccg acttattacg caaaatgcag tgtcatattt tgcagggatt ttatttcagc
      241 aaacccttgc cggtagcgca ggcggaacaa ttactcaaaa actggctaca actgagttag
      301 tcagcgagat tcagctgccg tcttgtcttc atttatctgc tgcgatacct cagccgctga
      361 cgtcagcggt ttggcatcca tcatttgggc gacccgtttc agcgatgaaa tgatcatggt
      421 ttgttcccat tcctgcaatt gactgaattc acgggtgaaa cgatcctgca gcggaatcgg
      481 cgcatttttc agcatcgtca tggcttcatc agtcatatag gcgtggactt tgcgtttatc
      541 ggtctgcgaa cgttgccgat acaccagttg gcgtttttcc agccggtcta aaatagtggt
      601 gacggttgcc tgactcagac tcatatcctg cgccagttca ccaatagtta actggccctt
      661 atcacgcaaa gtttgtagta acaaaatctg tggcgccgtc aaactggtgg ttttaaccag
      721 atactttgag tgcaagtcgg tggcccgaat cacccgccgt aaagccacca gtacttcctc
      781 aatctgatcg agcaaatgtt cccgttgggt gggttttaac cccagcgtgt cgatactggt
      841 tgtattgcga ttgtctgact tttccatctg ttgcacacct cgttgactga tttatgccta
      901 agtgcattgc attcatgtgt tttaaaacgc atgtaactta attgacatct aatcgcttta
      961 tcttaaaagc tgtcattttg acatttgatt tgtttagtgt actatagatt taattgataa
     1021 gcgatattga tgttaaacaa ttgattaaat gaaatattta acctgaattt aatcctgaat
     1081 caaaattaaa gtgccacgtt tattaaagta aactaaataa tagagttgat tacatatgca
     1141 agcagttaaa caaaacactt ccccgatcac cttaatccag ccaaccgctg aaatcggcgc
     1201 gcaggtgtat caattaattg ccaattgtcc gccactggat accaactcta tgtattgcaa
     1261 tttgttgcaa agcagtcatt ttgcagaaac agccgttgct gccatgctgg atgaagaagt
     1321 ggtgggcttt atctcaggct atcggatacc gcaacgccct gacagtttgt ttgtctggca
     1381 ggttgcagtg ggggaaaagg ccagagggca ggggctggcg acgcagatgt tgaaagccat
     1441 tttatcgcgg cctgaaaatc aggatattaa acgaattgaa acaacgatca cccctgacaa
     1501 tcgtgcctca tgggcgttat ttgaaggact ggcgagaaag ctggatactg aaatttccag
     1561 cagtgtgatg tttgataaaa ctacacattt tgccgatcaa catgcatcag aaatgctggt
     1621 gaaagtgggg ccgttcaagt cccagaaact gtaagcacca gcacgaaata agtattagaa
     1681 aaattatcag cttaggagat taccgttaat gaaaattttt gaagaaatcg aatcagaagt
     1741 ccgttcttat gcccgtgcct ttccccgtgt atttgaccgt gctcagggtg ccttaattta
     1801 tgatcgtgaa ggtaacgaat atctcgattt tctggcggga gcaggttcgc tgaactacgg
     1861 ccacaatcac cctatcttta aagaaaagct gattgaatat attcaggccg atggcatcac
     1921 tcaggggctg gatttacaca cccgtgccaa aggggagttt ttagaaagct tcaatgaaca
     1981 tattctgaag ccacgcaatt tggattatgt ggtcatgttt accggcccca caggcaccaa
     2041 tgcggtagaa gcagcattga agattgcccg caaaaaaacc ggtcgtgaaa acgttatttc
     2101 attcaccaat ggctggcatg gtcagacgct gggtgccttg tcagtgacag gtaactcaac
     2161 ccaccgtggc ggcgcaggta ttgccttgca tggctctacc cgtattcctt atgacggcta
     2221 cctaggtgat gattttgata ctaccaatct gctcgacaaa atgctgtcag attccagcag
     2281 tggtgtcgat aaacctgcag cagttatcgt tgaaaccgtg cagggtgaag gcggtattaa
     2341 tgcagcctcg atgacctggc tcaagagcct gtcagaaatc tgcaaacgtc atgatgtatt
     2401 gctgattatt gatgatattc aagcgggctg tggccggacc ggtaccttct tcagttttga
     2461 agaagccggc atttatccgg acattgttac cttgtcgaaa tcattaagcg gttatggttt
     2521 accgttcgcc attgtgctga tgaaaccaga agtggatcaa tggtcaccgg gtgaacataa
     2581 cggtactttc cgcggcaata accatgcgtt tgtcaccgcc aaagcggctt tggatcattt
     2641 ctggaaagac gataaatttg ccaaggaagt gcaacgcaag ggcgattata ttgccaagcg
     2701 ggtcgattcg attgtggcgg aatatggtga aggtaacttc aattcacatg gccggggcat
     2761 gttccgcggt atcaactgtg taaacggtga attggccgga caaatcacgc gcaagtgttt
     2821 ccagaaaggt ctgattatcg aaaccagtgg tgcagatgat catgtggtca aatttctttg
     2881 cccattgatt atcaccgatg aacagctgac aaaaggcatt gatattcttg aggaagctat
     2941 taaagaagtg tgtgctaaag cagattcaat tcctgaagaa aaagactttt tcgaaggcga
     3001 ttattccgtc agtgatgaag tgaataaagc gagctaattt cgactttttc taatttgcac
     3061 agactaaata aaggggtcat ttatgatcgt cagacaactt caagaagcac aaaaatcgtc
     3121 acgtcgtatc gtgtcaccgg atggtaattg ggaaagcaca cgcatgttgt tgaaagatga
     3181 caatatgggc tattccttcc atatcacaac gatatttgcc ggtgccgatt ttcggatgca
     3241 ttaccaaaac cacctggaat cggtgtattg catcagtggt gagggtgagg ttgagagcct
     3301 ggatgatggc aaaatctatc cgataacccc cggcactttg tacaacctgg atcagcacga
     3361 tcggcatatt ttgcgtgctt ttaaagagtt acatctggcc tgcgtattca atccaccgtt
     3421 aaatggtaaa gaagtgcaca acaaagaagg cgcttatgag ttggatgctg agtcagtaac
     3481 tgaataattt tcaacagatt gggtgactgg ctttgcggtc actcgccctt tttgctgaaa
     3541 cagtaagaga ggatattatg gattttcata ccgttgaaaa aattggcggc acatcaatga
     3601 gtgattatgt tgccgtgcgt gacaatgtca ttttaaagcc agttcattca acgtctttgt
     3661 atcaacgcat ttttgtggtt tcggcttacg ggggcattac caacctgtta ctggaacata
     3721 aaaagaatgg tcaggcgggt atttatggcc agtttgccaa cagcgtcaat gatgatggtt
     3781 ggcaagcgtc tttggagcag ttgaaacaag aaatgctggc gattaatgcc accttgtttg
     3841 atagcgcaga aaccattgcc aaagcggatg cctttatcag cgagcgtctg gatgataccg
     3901 aacgctgttt agcggatctg caacggctgt gtcagcatgg ccatttttct ttagatggtc
     3961 acctcgccac tgtacgggaa atgctggcca gtatcggtga agcccacagt gcctggaata
     4021 ctgttcagct attacagcag gatgatatta atgcggtgtt tgtggatttg accggttggc
     4081 aaaccgataa gcatatgacg ctggatgaac ggatcgaaac ggcttttgcc aagattgatc
     4141 tcaataccca attacccatt gtcaccggtt atgctcacag tattgatgga ttgatgtcga
     4201 cctttgaccg gggttacagc gaaatgacgt tcagtcgttt agcggtgtta accaatgcga
     4261 atgaagccat tattcataaa gaattccatt taagcagtgc ggatccgcgc ttggtgggcg
     4321 aagacaatgc ggtgccgatt ggccgtacca actacgatgt agccgaccag ctggcaaatc
     4381 tggggatgga agccattcat ccgaaagcgg ccaaaggtct gcgtaaaaat gagattccgt
     4441 tacgggttcg taataccttt gaaccagaac atgcgggcac gttgattact ggtgattatg
     4501 tcagcagcaa gccctgtgtt gagattattg ctggttgtaa aggcgtttat gcttttgaag
     4561 tatttgatca ggatatggca ggcgaaattg atagctacga caaagagatc ctggcgcaaa
     4621 tccgccgctt taaagcgcat atcgtctcta aagacattaa tgccaatacc attacgcatt
     4681 accttaccgc gagtctgaaa aaaatccgcc gtattcgtga tgcgctgcag gaacgttttc
     4741 cggatgctga aatcaatcag cagaaagtgg cgattgtatc ggccattggc agtgatatga
     4801 aaatccccgg cattctggca aaaacggtcg cggcattggc agagaaaaat atcagtgtac
     4861 tggcgatgca t
//
