LOCUS       BGC0000858              4253 bp    DNA     linear   BCT 30-DEC-2011
DEFINITION  Methylobacter marinus ectoine biosynthesis gene cluster, complete
            sequence; and transporter solute:sodium symporter (SSS) family
            protein (ssf) gene, complete cds.
ACCESSION   BGC0000858
VERSION     BGC0000858
KEYWORDS    .
SOURCE      Methylobacter marinus
  ORGANISM  Methylobacter marinus
            Bacteria; Proteobacteria; Gammaproteobacteria; Methylococcales;
            Methylococcaceae; Methylobacter.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-07 20:50:35
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4253
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..4253
                     /db_xref="taxon:34058"
                     /mol_type="genomic DNA"
                     /organism="Methylobacter marinus"
                     /strain="7C"
     protocluster    1..4253
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[1963:2371]"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1964..2371
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     cand_cluster    1..4253
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..4253
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000858"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..4253
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            214..693
                     /gene="ectA"
     CDS             214..693
                     /codon_start=1
                     /gene="ectA"
                     /product="L-2,4-diaminobutyric acid acetyltransferase"
                     /protein_id="ACB30326.1"
                     /transl_table=11
                     /translation="MTDGPSMQALVEASPPLDNNSRYCYLLLCEYFADTCAVVEADGEI
                     LAFVTAYVPPGKPDTLAVWQVAVASSLRGQGVARQLIDHVLRRPGLEHIRFVEATVNPS
                     NDASRALFASLAKACETTMSEACIFPKTLFPSGHEQENLLRVGPFQIHTAIQEQS"
     misc_feature    214..2371
                     /note="ectoine biosynthesis gene cluster"
     gene            690..1967
                     /gene="ectB"
     CDS             690..1967
                     /NRPS_PKS="Domain: Aminotran_3 (22-356). E-value: 1.7e-80.
                     Score: 262.4. Matches aSDomain:
                     nrpspksdomains_ectB_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013:aminotransferase class-III (Score: 397.3;
                     E-value: 1.3e-120)"
                     /gene_kind="biosynthetic-additional"
                     /product="L-2,4-diaminobutyric acid aminotransferase"
                     /protein_id="ACB30327.1"
                     /sec_met_domain="Aminotran_3 (E-value: 1.9e-83, bitscore:
                     268.4, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRIFEEMESEVRGYIRSFPAVFDTARGSEMFDERGNRYIDFFAGA
                     GTLNYGHNNPAISQALIDYLQNDGIVHGLDKATVAKKVFLQKLADTIFAPRNLEYKVQF
                     TGPTGTNAVETALKLARMVKQRSNVIAFTNAYHGLSMGSLAITGNSFYRDESYGVRHNS
                     AFVPYDGYFGQDVNTIDYLRRFLMDQSSGIDLPAAIIVETVQGEGGIHVASDAWLRELE
                     SLCREFDILLVLDDIQMGNGRTGTFFSFERAGITPDMVTVSKSIGAGLPLSLLLMRPEL
                     DQWKPGEHTGTFRGNNLAFVAATKALEYWDTPDFAEGVRHKGEIFRESLQAIVDKHPAI
                     NGELRGVGMVWGIEFADPGFAGAVSEEAFKQGLVIETAGANGQVLKFLPALTMDEAIIT
                     EGLAIIEKSIEAVLEKRKSLRAGSLS"
     aSDomain        756..1757
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ectB_Aminotran_3.1"
                     /evalue="1.70E-80"
                     /label="ectB_Aminotran_3.1"
                     /locus_tag="ectB"
                     /protein_end="356"
                     /protein_start="22"
                     /score="262.4"
                     /tool="antismash"
                     /translation="DTARGSEMFDERGNRYIDFFAGAGTLNYGHNNPAISQALIDYLQN
                     DGIVHGLDKATVAKKVFLQKLADTIFAPRNLEYKVQFTGPTGTNAVETALKLARMVKQR
                     SNVIAFTNAYHGLSMGSLAITGNSFYRDESYGVRHNSAFVPYDGYFGQDVNTIDYLRRF
                     LMDQSSGIDLPAAIIVETVQGEGGIHVASDAWLRELESLCREFDILLVLDDIQMGNGRT
                     GTFFSFERAGITPDMVTVSKSIGAGLPLSLLLMRPELDQWKPGEHTGTFRGNNLAFVAA
                     TKALEYWDTPDFAEGVRHKGEIFRESLQAIVDKHPAINGELRGVGMVWGIEFA"
     CDS_motif       1659..1802
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ectB_0001"
                     /evalue="2.80E-03"
                     /label="C2_DCL_024-062"
                     /locus_tag="ectB"
                     /protein_end="371"
                     /protein_start="323"
                     /score="10.0"
                     /tool="antismash"
                     /translation="EIFRESLQAIVDKHPAINGELRGVGMVWGIEFADPGFAGAVSEEA
                     FKQ"
     gene            1964..2371
                     /gene="ectC"
     CDS             1964..2371
                     /codon_start=1
                     /gene="ectC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /product="ectoine synthase"
                     /protein_id="ACB30328.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.9e-51, bitscore:
                     161.5, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSLQDIIGTERDVKAENGNWESRRLLLNNDALGFSLHDTIIH
                     AGTETLIWYKHHLEAVYCIEGEGEVETTENGKIYPIKAGTVYALNGNERHLLRAHSTMR
                     MVCVFTPPITGSEVHDQDGAYRPGAEIAAGQ"
     gene            2454..3827
                     /gene="ssf"
     CDS             2454..3827
                     /codon_start=1
                     /gene="ssf"
                     /note="Na+/proline symporter"
                     /product="transporter solute:sodium symporter (SSS) family
                     protein"
                     /protein_id="ACB30329.1"
                     /transl_table=11
                     /translation="MLSALKSKGSAEDYLTAGKSVPAWLAGLSAVATNNSGFMFIGMIG
                     LTYSTGLSSIWLMLGWIAGDLAASLSIMRRLREVSASPGIHSFGALLARWHDTDFRWLQ
                     RLAGALTILFLGAYSAAQLKAGSKALHVLFGWDASTGALIGAVMVILYCFAGGIRASIW
                     TDAAQSFVMLAAMLVMVFTGLQQLDGFGGVYPALLQVSPTYMNWFPETDGWRPVLFVLG
                     WVFAGFGVAGQPHIVIRFVSLDHPQSIGRMRLYYYSWFVMFYSLTIAAGMLARLLLPES
                     AAFDAELALPTLAMEMLPPVLIGLFLAGLFAATMSTVDSLIIACTAAFTRDFHGARTPG
                     IFITKLVTLSVVAVALGFALSDSRSVLSLVLISWGLLASAFAPLLLVYAFDGKPDEKLA
                     ISMLLVGVAVFLLWRHFGLSQLANSIMPAMLSGMAMYAIGKTMGWTLENTGGERAGIL"
ORIGIN
        1 cgttttcagg tatttttatt tttccggtct gttggcggca aagagggctt gaatggctat
       61 gatcatgaat gggggcggtt tgtttgacgc tgattgatgt gttagcatga aaaagaatga
      121 attattcaga aatggtgaac gactatccct tattcgaaag ccttgaagcg gcaatcctct
      181 caaattaaca acctcgtttt tcgtcctccc gtcatgactg acggtccttc catgcaagct
      241 ctggtggaag cgagcccgcc cctggataac aactccagat actgttacct gctcctgtgc
      301 gagtacttcg cggatacctg cgcggtcgtt gaagccgacg gcgaaatcct cgccttcgtc
      361 accgcctatg tcccccccgg caagccggat acgctggccg tctggcaagt tgccgtggcg
      421 tcttcgttgc gcggacaggg cgtggcccgc caattgatcg atcatgtgct gagacggccc
      481 ggtctcgaac atatccgatt tgtcgaagcc acggtcaatc cgtcgaatga tgcgtcccgc
      541 gcgctgtttg cctcgttagc caaagcttgc gagacaacga tgagcgaggc ctgcattttc
      601 cccaagacgt tattccccag cggccatgag caggaaaacc tgctcagggt aggaccgttc
      661 cagattcaca cagcaataca ggagcaatca tgagaatttt tgaagaaatg gaatccgaag
      721 tgcgcggcta tatccgctca ttcccggccg tgttcgacac ggccagggga tcggagatgt
      781 ttgatgagcg cggcaaccgc tacatcgatt ttttcgccgg tgcgggcacg ctcaattacg
      841 gccataacaa tccggctatc agtcaggcgc tcatcgacta tctgcagaat gacggcattg
      901 tccacggcct ggacaaggcg accgtggcca aaaaggtttt cctgcagaag ctggccgata
      961 ccattttcgc gccgcgcaac ctggaataca aagtgcagtt taccggcccg accggaacca
     1021 atgcggtcga gaccgcgctc aagctggcgc gcatggtcaa gcagcgctcc aacgtgatcg
     1081 ctttcaccaa tgcctatcac ggcctgtcca tgggctcgct ggcgatcacc ggcaattcat
     1141 tttaccgcga cgaatcctac ggcgtgcgcc ataacagcgc cttcgtgccg tacgacggct
     1201 acttcggcca ggatgtcaat accatcgatt acctgcgccg cttccttatg gaccagagca
     1261 gcggtatcga tctgccggcg gccattatcg tcgaaaccgt gcagggcgag ggcggcatac
     1321 acgtcgccag cgacgcatgg ctgcgcgaac tcgagtcgct gtgccgcgaa ttcgacattc
     1381 tgctggtcct ggacgacatc cagatgggca acggccgcac cggcaccttt ttcagtttcg
     1441 aacgcgccgg catcacgccg gacatggtga ccgtgtccaa atccatcggc gcgggcctgc
     1501 cattgtcgct gttgctgatg cgcccggagc tggaccaatg gaagccgggc gagcataccg
     1561 gtacgttccg cggcaataat ctcgccttcg tcgccgcaac caaggctctg gaatattggg
     1621 ataccccgga tttcgccgaa ggcgtcaggc acaagggcga gatcttcagg gaaagtctgc
     1681 aggccattgt cgacaaacat ccggccatca acggcgaact gcgcggcgtc ggcatggtct
     1741 ggggcatcga attcgccgat ccgggtttcg ccggcgccgt gtccgaagaa gcattcaagc
     1801 aaggactggt gatcgaaacg gccggggcca acggccaggt gctgaagttc ctgccggcgc
     1861 tgaccatgga cgaagcgatc atcacagagg gattggctat catcgagaag tcgatcgagg
     1921 ccgtgctgga aaaacgcaag tcgctgcgtg ccgggagcct gtcatgatcg tacgttcact
     1981 gcaagacatc atcggcaccg agcgcgatgt caaagctgaa aacggcaact gggaaagccg
     2041 ccgtctgctg ctcaacaacg atgcgctggg cttttccctg cacgacacca taatccatgc
     2101 cggcaccgaa acgctgatat ggtacaagca tcacctcgaa gccgtgtact gcatcgaagg
     2161 cgagggcgag gtcgaaacca ccgagaacgg caagatctac ccgatcaagg cgggcacggt
     2221 ctatgccctg aacggcaacg aacgccacct gcttcgggcg catagcacga tgcgcatggt
     2281 ttgtgtcttc acgccgccga ttaccggcag cgaagtccat gatcaggacg gcgcctatcg
     2341 cccgggggca gagatagcgg ccgggcaata acgtccggcc gcggctagca ggagggactg
     2401 ccatggtcat tgccagcttt attttctttt tattgatctt cgtcgcgata ggcatgctct
     2461 cggcgttaaa gagcaagggc agcgccgagg attacctgac ggccggcaag agcgtgccgg
     2521 cctggctggc cgggctttcc gccgtggcca ccaataacag cggcttcatg ttcatcggca
     2581 tgatcgggct gacctattcc accgggttgt cgtcgatctg gctcatgctg ggctggattg
     2641 ccggcgatct ggcggcctcg ctcagcatca tgcgccgctt gcgcgaggtt agcgcaagcc
     2701 ccggcattca ttccttcggc gctttgctgg cgcgctggca cgacacggat tttcgctggc
     2761 tgcaacgcct tgccggcgcg ttgacgatcc tgttcctggg tgcttattcc gccgcccagc
     2821 tcaaggcggg cagtaaggcg ctgcacgtgt tgttcggctg ggacgcctcg acgggagccc
     2881 tgatcggcgc cgtcatggtg atcctttact gtttcgcggg cggcattcgc gcctccatct
     2941 ggaccgatgc ggcgcaatcc tttgtcatgc tcgccgccat gctggtaatg gtgtttaccg
     3001 gcctgcagca actggacggt ttcggcggcg tttatccggc gctgctgcaa gtatcgccga
     3061 cttacatgaa ctggtttccg gaaacggacg gctggaggcc ggtactgttc gtgctcggct
     3121 gggtatttgc cggctttggc gtggcgggtc agccgcatat cgtcatccgt ttcgtcagcc
     3181 tcgaccaccc gcaatcgatc gggcgcatgc gcctgtatta ttacagctgg tttgtcatgt
     3241 tctattcgct gaccatcgcc gcgggcatgc tggcgcgcct gctgttaccc gaatcggccg
     3301 cgttcgatgc ggaactggcg ctgcccacgc tggccatgga gatgctgccg ccggtgctga
     3361 tcggactttt tctggccggg ctgtttgccg cgaccatgtc caccgtcgac tcgctgatca
     3421 ttgcctgtac cgccgcgttt acccgcgatt tccacggcgc acggactccc gggattttca
     3481 tcaccaaact ggtgacgctt tcagtcgtgg cggtcgccct gggcttcgcg ttgtcggaca
     3541 gccgcagcgt gctgagtctg gtactgatct cctgggggct gctcgcatcg gcgttcgcgc
     3601 ctttattgct ggtgtatgcg tttgatggca agcccgacga gaaactggcg atatcgatgc
     3661 tgctggtcgg cgtcgccgta ttcctgctct ggcgtcattt cggcctgtcg cagctggcca
     3721 atagcatcat gcccgccatg ctgtcgggca tggcgatgta tgccatcggc aaaaccatgg
     3781 gctggacgct cgaaaacacg ggcggggagc gggccggaat cctgtgacca agccggtgag
     3841 tcgctgaggc aagagaatac gcttcccgca actacctctc atcgatggct tcatcaggcc
     3901 gcctggcttt tcgcttcgat aatggcctga atctcgggcg cttccagggt ctctttctcc
     3961 agcagcgttg cggccagggc ttccagtttc gggcggtgtg tctttagcag ttccgtgatc
     4021 tgatcttcaa tgccgtgaac cagcatgcgg atctcatcgt cgatgatctg cgcggtatgt
     4081 tcgctgaaat cgcgctgctg ggtcatttcc cggcccagga agatgtgttc ctcgccccgg
     4141 cggaaggcga ccgggccgag tttggcgctc atgccccaat gggtcaccat gtggcggagc
     4201 cagccgggtg tgcctgtttg agtatcttgc gtgcggcgac cggaactgaa ttc
//
