LOCUS       BGC0000860              5134 bp    DNA     linear   BCT 30-DEC-2011
DEFINITION  Methylarcula marina strain h1 ectoine biosynthesis gene cluster,
            complete sequence; and aspartokinase (ask) gene, partial cds.
ACCESSION   BGC0000860
VERSION     BGC0000860
KEYWORDS    .
SOURCE      Methylarcula marina
  ORGANISM  Methylarcula marina
            Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
            Rhodobacteraceae; Methylarcula.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-07 20:50:52
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..5134
                     /db_xref="taxon:72022"
                     /mol_type="genomic DNA"
                     /organism="Methylarcula marina"
                     /strain="h1"
                     /type_material="type strain of Methylarcula marina"
     protocluster    1..5134
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[3341:3737]"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      3342..3737
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     cand_cluster    1..5134
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..5134
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000860"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..5134
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            complement(711..1265)
                     /gene="ectR"
     CDS             complement(711..1265)
                     /codon_start=1
                     /gene="ectR"
                     /gene_functions="regulatory (smcogs) SMCOG1135:MarR family
                     transcriptional regulator (Score: 72.7; E-value: 3.7e-22)"
                     /gene_kind="regulatory"
                     /note="EctR"
                     /product="MarR family transcriptional regulator"
                     /protein_id="ADA71973.1"
                     /transl_table=11
                     /translation="MVCRLNGPGISCDDRIMSKPADNRLDEALIALRRILRATELYERE
                     LAQAARLTPAKLRVLQILASREGGSATPTALANQMGVSQATVTALVDQLQRREMVSRQR
                     SSMDRRQTNVVIAQAGTEALKNAPNALQQHFVKNFEKLETWEQQMVLAVLDRVAGMLNA
                     GEIEASPVLAIGDIRKDRDRL"
     misc_feature    711..3737
                     /note="ectoine biosynthesis gene cluster"
     gene            1441..1986
                     /gene="ectA"
     CDS             1441..1986
                     /codon_start=1
                     /function="catalyzes the acetylation of
                     L-2,4-diaminobutyrate (DABA) to
                     gamma-N-acetyl-alpha,gamma-diaminobutyric acid (ADABA) with
                     acetyl coenzyme A"
                     /gene="ectA"
                     /note="EctA"
                     /product="L-2,4-diaminobutyric acid acetyltransferase"
                     /protein_id="ADA71974.1"
                     /transl_table=11
                     /translation="MPKDVNEIEKHRNRTIVLRKPRSTDGSAIWDLVRACKPLDENSMY
                     CNIVQAEHFRDTCVVAEMNGDIVGWISGHMIPNEDALFVWQVAVSPRARGLGLGRKMLE
                     HLIGRDACAEAQQLNTTITKDNEASWALFRSFAREVGGELSDKAHYKRDLHFDGQHATE
                     HMVTITLPEEAGATSAAA"
     gene            2042..3334
                     /gene="ectB"
     CDS             2042..3334
                     /NRPS_PKS="Domain: Aminotran_3 (31-364). E-value: 1.8e-67.
                     Score: 219.6. Matches aSDomain:
                     nrpspksdomains_ectB_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013:aminotransferase class-III (Score: 322.7;
                     E-value: 5.5e-98)"
                     /gene_kind="biosynthetic-additional"
                     /note="EctB; catalyzes the conversion of aspartate
                     semialdehyde into L-2,4-diaminobutyric acid"
                     /product="L-2,4-diaminobutyrate transaminase"
                     /protein_id="ADA71975.1"
                     /sec_met_domain="Aminotran_3 (E-value: 6.4e-73, bitscore:
                     234.0, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPKDMTNGTDIGIFERRESAARSYCRGIPAVFTAARGSILTAQDG
                     SEYIDFLAGCSSLNYGHNDADMKAALVEHITADGLAHGLDLHTDTKAGFLSAFERHILV
                     PRGMDHRVMFTGPTGANAVEAAMKIARKSTGRTNIVAFTNGFHGVTMGALAATGNGYHR
                     GGAGMDKQGVTRMPYDGYADGVDGAALLDQMLSDASGGIDAPAAIMLETVQGEGGLNAA
                     SGGFVRKIAGVARKHGALLIIDDIQAGCGRTGTFFSFEEMGVTPDIVTMAKSISGFGLP
                     LALVLVRPEHDVFGPAEHNGTFRGNTHAFVTARVAIEKFWAAKSFETELAGKAALIEKR
                     LTELAALVPGAFLKGRGLMRGVDVGSGDLAGKICARAYEKGLVIETSGSEDQVVKVLAP
                     LTTPVETFEKGFDILLDAAREILDMTRIAAE"
     aSDomain        2135..3133
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ectB_Aminotran_3.1"
                     /evalue="1.80E-67"
                     /label="ectB_Aminotran_3.1"
                     /locus_tag="ectB"
                     /protein_end="364"
                     /protein_start="31"
                     /score="219.6"
                     /tool="antismash"
                     /translation="FTAARGSILTAQDGSEYIDFLAGCSSLNYGHNDADMKAALVEHIT
                     ADGLAHGLDLHTDTKAGFLSAFERHILVPRGMDHRVMFTGPTGANAVEAAMKIARKSTG
                     RTNIVAFTNGFHGVTMGALAATGNGYHRGGAGMDKQGVTRMPYDGYADGVDGAALLDQM
                     LSDASGGIDAPAAIMLETVQGEGGLNAASGGFVRKIAGVARKHGALLIIDDIQAGCGRT
                     GTFFSFEEMGVTPDIVTMAKSISGFGLPLALVLVRPEHDVFGPAEHNGTFRGNTHAFVT
                     ARVAIEKFWAAKSFETELAGKAALIEKRLTELAALVPGAFLKGRGLMRGVDV"
     gene            3342..3737
                     /gene="ectC"
     CDS             3342..3737
                     /codon_start=1
                     /function="catalyzes the cyclic condensation of
                     gamma-N-acetyl-diaminobutyric acid into ectoine"
                     /gene="ectC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /note="EctC"
                     /product="L-ectoine synthase"
                     /protein_id="ADA71976.1"
                     /sec_met_domain="ectoine_synt (E-value: 6.7e-49, bitscore:
                     153.6, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRDFNKLKDTDRSVSDARWNSVRMLLADDGMGFSFHITTLEAG
                     SEHTFHYKHHFESVYCMSGKGSITDLATGETHEIKPGVMYALNLHDKHTLRAEEELVMA
                     CCFNPPVTGKEVHREDGSYAPVEELTD"
     gene            3740..>5134
                     /gene="ask"
     CDS             3740..>5134
                     /codon_start=1
                     /function="catalyzes the formation of 4-phospho-L-aspartate
                     from L-aspartate and ATP"
                     /gene="ask"
                     /note="Ask; associated with ectoine biosynthesis"
                     /product="aspartokinase"
                     /protein_id="ADA71977.1"
                     /transl_table=11
                     /translation="MTHTVEKIGGTSMSRVNELCDTLIIGDRKGADLYGRILVVSAFGG
                     ITDLLLEHKKSGKAGVYAAFASSDSDHGWHEALDRVSDAMVAAHKAVLDHPGDIERADA
                     FVHDRILGARNCLIDLQRLCTYGHFRLSEHMLQTRELLSGLGEAHSAFVTTLMLQRAGV
                     NARFVDLSGWRDEGDVSLDERIKGAMKDVDPATEMPIVTGYAQCAEGLMREFDRGYSEV
                     TFSRLASLTGAREAIIHKEFHLSSADPKLVGQDAVRKLGRTNYDVADQLSNLGMEAIHP
                     KAAKTLRQSGVPLRVTNAFEPEDPGTLIDDQPADGPNVEIVTGLDLFALELFEQDMVGA
                     KGYDSAVLDILTRHNVRIVSKVSNANTVTHYVDSSLKVLRRIEKDLITRYPSARVTSRA
                     ISMVAAVGRDLNGLAVLSRGLNALADHGLEAIGATQGPRPVDVQFIVEREVMKPAIKAL
                     HHALIEN"
ORIGIN
        1 cgcatggcct cggggcattt ccagcgcggg atgaagctga cccatgtgcg cacgaaaaag
       61 ccgatggcgg tgtcgaaccc ggtgctgttc cttgccgccg accgggaact ggccgaagag
      121 gcttgggcgg gcgatatcat cggcatcccg aaccatgggc agttgcgcat cggcgacgcg
      181 ttgaccgagg gcgaggcgct gcgtttcacc ggcattccgt cattcgcgcc ggaactgctg
      241 caatcggtgc gtgccacgga cccgatgaag gccaagcacc tggaaaaggc gctgatgcaa
      301 ttcgccgaag agggcgcggc gaaggtgttc aagccagcga tcggctcggg cttcatcgtc
      361 ggcgtggtcg gcgcgctgca attcgaggtt ctggccagcc ggatcgagct ggaatacggc
      421 atcccggtgc ggttcgaggg ctcgcaattc acctctgccc cgatgggtgc aggggccgaa
      481 ggacaaggtc gatacctttg ccaatgccaa caaggggcat atggcccatg accatgacgg
      541 cgatcttgtc tatctgaccc ggctgcaatg ggatatcgac cgcatcgagc gcgaccatcc
      601 cgatctgaag ctgaccgcga ccaaggagat gatggtctga acgcatcgcc cggcgtgggg
      661 cggtgcgaag gggttgcggc tgtgaacctg tcttccggta tcgtccggga tcacaggcgg
      721 tcgcgatcct tgcggatatc gccgatggcc agcaccgggg aggcctcgat ctcaccggca
      781 ttcagcattc ccgccacccg gtccagaacg gcaaggacca tttgctgttc ccaggtttcg
      841 agcttctcga aattcttgac gaaatgctgc tgcagggcgt tcggggcgtt tttcaacgct
      901 tcggtgcccg cttgcgcgat gaccacgttg gtctgcctgc gatccatgct ggaccgctgg
      961 cggctgacca tctcgcgcct ttgcaactgg tcgaccaggg cggtcaccgt cgcctggctg
     1021 acacccatct gattggccag cgccgttggg gtggcgctgc cgccttcgcg gctggccagg
     1081 atttgcagca cgcgcagctt ggccggtgtg agccgggcgg cttgcgccag ttcgcgctcg
     1141 taaagttcgg tcgcccgcag gatgcgacgc aacgcgatca gggcttcgtc caggcggttg
     1201 tcggcgggtt tgctcatgat gcgatcatca catgaaatgc ccgggccgtt caatctgcaa
     1261 accatcggga aaatattttg cttgactaag ctgtttttgg gggctcttcg aaggaaattc
     1321 cgtcaaaagc ttcgtaatgc cagccattta actgatattg gctgaatttt gccataacgg
     1381 ttgcaaccat ggggcggctt cattagttag taggacaaag caaaacagag atcggattcc
     1441 atgcccaaag acgtgaacga gatcgaaaag caccgcaacc ggacgattgt cctgcgcaag
     1501 ccgcgcagca ccgacggctc cgccatctgg gatctggtgc gcgcgtgcaa gccgctggac
     1561 gagaactcga tgtattgcaa tatcgttcag gccgagcatt tccgcgacac ctgcgtggtg
     1621 gcggaaatga atggcgatat cgtcggctgg atttcgggtc acatgatccc gaacgaggat
     1681 gcgctgttcg tctggcaggt ggctgtcagc ccgcgtgccc gtgggctggg acttggccgc
     1741 aagatgctgg agcatctgat cggccgcgat gcctgtgccg aggcgcagca gctcaatacc
     1801 accatcacca aggataacga ggcctcctgg gcgctgttcc gcagctttgc ccgcgaggtg
     1861 ggtggggagc tttccgacaa ggcgcattac aagcgcgatc ttcatttcga cggccagcac
     1921 gccaccgagc atatggtgac gatcaccctg ccggaggagg cgggcgcgac ttcggccgca
     1981 gcctgatctg cgcccaacag gccgacccca tcaagacgca caaggtgcga aaggacttct
     2041 tatgcccaag gacatgacca acggtaccga tatcggtatt ttcgagcgcc gcgaaagcgc
     2101 ggcgcgttcc tattgccggg gtattccggc tgttttcact gccgcgcgcg gctcgatcct
     2161 gacggctcag gacggatcgg aatatatcga tttcctggcc ggttgttcct cgctgaatta
     2221 cggccataac gatgcggata tgaaggccgc gctggtcgag catatcaccg ctgacgggct
     2281 ggcccatggg ctggatctgc acaccgatac gaaagccgga ttcctgtcgg cattcgaacg
     2341 tcatatcctt gtgccgcgcg ggatggatca ccgggtgatg tttaccggcc cgaccggcgc
     2401 gaatgccgtc gaggcggcga tgaagatcgc acgcaaatcg actggccgca cgaatatcgt
     2461 cgccttcacc aatggcttcc atggtgtgac catgggggcg ctggcggcga ccggcaacgg
     2521 ctatcaccgt ggcggcgcgg gcatggacaa gcagggcgtc acccggatgc cctatgacgg
     2581 ctatgccgat ggtgtcgacg gggccgcgct gctggaccag atgctgtccg acgcctcggg
     2641 cgggatcgac gcacccgccg ccatcatgct ggagaccgtg cagggcgagg gcgggctgaa
     2701 cgccgcgtcg ggcgggttcg tccggaaaat cgccggggtc gccaggaagc atggcgcgct
     2761 gctgatcatt gatgatatcc aggccggttg cggccggacc ggcaccttct tctccttcga
     2821 ggagatgggc gtcacacccg atatcgtcac catggcgaaa tccatctcgg gcttcggcct
     2881 gccgctggca ctggtcctgg tgcggcccga gcatgacgtc tttggtccgg ccgagcataa
     2941 cggcactttc cggggcaata cccatgcctt cgtcaccgcc cgtgtcgcga tcgagaaatt
     3001 ctgggccgcc aagagcttcg agaccgagct ggccggaaag gccgcgctga tcgaaaagcg
     3061 gctgaccgag ctggccgctc tggttcccgg agccttcctg aagggccgtg ggctgatgcg
     3121 cggcgtcgat gtcggatccg gggatctggc cgggaaaatc tgcgcacgcg cctatgagaa
     3181 ggggcttgtg atcgagactt cgggcagcga agatcaggtc gtgaaggtcc tggccccgct
     3241 gaccaccccg gtggagacat tcgaaaaggg ttttgacatc ctgctggatg cggcccggga
     3301 aattctcgac atgaccagga ttgcagcgga gtaagacaga gatgatcgtt cgcgacttca
     3361 acaagttgaa agacactgac cgttccgttt cggatgcgcg ctggaactcg gtgcggatgc
     3421 tgctggccga tgacgggatg gggttctcgt tccacatcac cacgctcgag gcgggatccg
     3481 agcatacttt ccactacaag catcacttcg aaagcgtcta ttgcatgtcg ggcaagggct
     3541 cgatcaccga tctggcgacc ggcgagacgc atgaaatcaa gcccggtgtc atgtatgcgc
     3601 tgaacctgca tgacaagcat accctgcggg ccgaggaaga gctggtcatg gcttgctgct
     3661 tcaacccgcc ggtaacgggc aaggaagtcc accgcgaaga cggctcttac gccccggtgg
     3721 aagaactcac ggactgaaca tgacccatac tgtcgagaaa atcggcggca cttcgatgtc
     3781 gcgcgtgaat gaattgtgcg acacgctgat catcggtgac cgcaaggggg ccgatcttta
     3841 cggacggatt ctggtcgtct cggccttcgg cgggatcacc gatctgctgc tggaacacaa
     3901 gaaatccggc aaggccgggg tctatgcggc cttcgccagt tcggattccg atcatggctg
     3961 gcacgaggct ctggaccgcg tctcggatgc gatggtcgcg gcgcataagg cggtgctgga
     4021 tcatcccggc gatatcgagc gcgcggatgc cttcgtgcat gatcgcatcc ttggcgcccg
     4081 caattgcctg atcgatctgc agcgcctgtg cacttacggc catttccgcc tgtccgagca
     4141 tatgctgcag acccgcgaat tgctgtcggg actgggcgag gcgcattcgg ctttcgtcac
     4201 cacgctgatg ctgcaacgcg caggcgtgaa tgcccgtttc gtcgacctgt cgggttggcg
     4261 tgacgagggc gatgtcagcc tggacgagcg tatcaagggc gcgatgaagg atgtcgatcc
     4321 ggcaaccgag atgcccattg tcaccggcta tgcgcaatgt gccgaggggc tgatgcgcga
     4381 attcgaccgt ggctattccg aggtgacctt ctcgcggctg gcatcgctga ccggcgcgcg
     4441 cgaggcgatc atccacaagg agttccacct gtcctcggcc gatccgaaac ttgtcgggca
     4501 ggatgccgtg cgcaagcttg gccggacgaa ttacgatgtc gccgatcagc tttccaacct
     4561 gggcatggag gcgatccatc ccaaggccgc caaaacgctg cgccaatcgg gcgtgccgct
     4621 gcgcgtgacc aacgcctttg agcccgagga ccccggcacg ctgatcgacg atcagcccgc
     4681 cgacgggccg aatgtcgaga tcgtgaccgg cctcgatctg ttcgcgctgg aactgttcga
     4741 acaggacatg gtcggcgcca agggctatga cagcgccgtg ctggatatcc tgacccggca
     4801 taacgtccgc atcgtctcca aggtctcgaa cgccaataca gtcacgcatt acgttgacag
     4861 ctcgctcaag gtgctgcgcc ggatcgagaa ggacctgatc acgcgctacc cttcggcgcg
     4921 ggtaacatcg cgcgcgatct cgatggttgc cgcggtgggg cgcgacctga acgggctggc
     4981 ggtgctgagc cgcgggctga acgcgctggc cgatcatggg ctggaggcga ttggcgcgac
     5041 gcaggggcca cgcccggtcg atgtgcaatt catcgtcgag cgcgaggtga tgaagcccgc
     5101 gatcaaggcg ctgcaccatg cgctgatcga gaat
//
