BGC0000865 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000865 (Photobacterium profundum SS9)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	PUFA,T1PKS,hglE-KS	1	33,099	High	eicoseicosapentaenoic acid	other:other

BGC0000865 - Region 1 - PUFA,T1PKS,hglE-KS

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Location: 1 - 33,099 nt. (total: 33,099 nt) Show pHMM detection rules used

Region on contig edge.

PUFA: PUFA_KS
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
hglE-KS: (hglE or hglD)

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 33,100
Label		BGC0000865

Tailoring functions

Transferases: acylase formyltransferase

pfaA: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

pfaD: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

purU: methionyl-tRNA formyltransferase

smcogs: SMCOG1228: methionyl-tRNA formyltransferase

Hydrolases: dehalogenase protease

AAL01057.1: haloalkane dehalogenase

smcogs: SMCOG1262: haloalkane dehalogenase

AAL01067.1: alkaline serine protease, subtilase family

smcogs: SMCOG1075: alkaline serine protease, subtilase family

Lyases: dehydratase

AAL01055.1: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Ligases: ligase synthase

AAL01056.1: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

fabH: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

pfaC: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000865.3	2.93	other (other)	eicoseicosapentaenoic acid	Photobacterium profundum SS9
BGC0002648.2	1.18	PKS	eicosapentaenoic acid	Shewanella sp. N2AIL
BGC0000861.5	1.17	other (other)	eicosapentaenoic acid	Shewanella sp. BR-2
BGC0000863.3	0.97	other (other)	eicosapentaenoic acid	Pseudoalteromonas sp. DS-12
BGC0000862.3	0.93	other (other)	eicosapentaenoic acid	Shewanella pneumatophori
BGC0001164.3	0.86	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001258.3	0.85	PKS	1,3,6,8-tetrahydroxynaphthalene	Glarea lozoyensis
BGC0001160.5	0.85	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001268.4	0.85	NRPS, PKS	fusarin C	Fusarium fujikuroi
BGC0001254.3	0.81	PKS	ACT-Toxin II	Alternaria alternata

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000865.3	0.99	other (other)	eicoseicosapentaenoic acid	Photobacterium profundum SS9
BGC0002648.2	0.71	PKS	eicosapentaenoic acid	Shewanella sp. N2AIL
BGC0000861.5	0.71	other (other)	eicosapentaenoic acid	Shewanella sp. BR-2
BGC0000863.3	0.66	other (other)	eicosapentaenoic acid	Pseudoalteromonas sp. DS-12
BGC0000862.3	0.64	other (other)	eicosapentaenoic acid	Shewanella pneumatophori
BGC0001164.3	0.62	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001258.3	0.62	PKS	1,3,6,8-tetrahydroxynaphthalene	Glarea lozoyensis
BGC0001160.5	0.62	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001268.4	0.62	NRPS, PKS	fusarin C	Fusarium fujikuroi
BGC0001254.3	0.61	PKS	ACT-Toxin II	Alternaria alternata

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 33099:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

AAL01056.1: X

AMP-binding (19..466): X

nrpys: Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: --FFIASPLK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

pfaA: mal

PKS_AT (598..900): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; inactive: 70.8%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 99.5
Methoxymalonyl-CoA: 31.9
inactive: 31.4
Methylmalonyl-CoA: 23.0
fatty_acid: 16.8
Isobutyryl-CoA: 13.6
Propionyl-CoA: 11.6
CHC-CoA: 10.5
trans-1,2-CPDA: 0.0
Ethylmalonyl-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

pfaB: pk

PKS_AT (326..630): pk

ATSignature: (unknown)

No matches above 50%

minowa: trans-1,2-CPDA

Prediction, score:

trans-1,2-CPDA: 0.0
inactive: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Methylmalonyl-CoA: 0.0
Methoxymalonyl-CoA: 0.0
Malonyl-CoA: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor