LOCUS       BGC0000866              5500 bp    DNA     linear   BCT 12-APR-2000
DEFINITION  Burkholderia sp. DSMZ 9242 polyhydroxyalkanoate biosynthesis gene
            cluster and unknown gene, complete cds.
ACCESSION   BGC0000866
VERSION     BGC0000866
KEYWORDS    .
SOURCE      Burkholderia sp. DSM 9242
  ORGANISM  Burkholderia sp. DSM 9242
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Burkholderia.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-07 20:52:49
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5500
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..5500
                     /db_xref="taxon:111590"
                     /mol_type="genomic DNA"
                     /organism="Burkholderia sp. DSM 9242"
                     /strain="DSM 9242"
     subregion       1..5500
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000866"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..5500
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     CDS             468..2345
                     /codon_start=1
                     /note="poly-hydroxyalkanoate polymerase"
                     /product="PhaC"
                     /protein_id="AAF23364.1"
                     /transl_table=11
                     /translation="MTASKNSSTSAQADTFAGQQAFAQAAQQAAQPMQQMFEAWLSAWR
                     GFADPARAATASAATNPFATFQFPKSFPFQMPSMADFGGAASPFAGLKLPAAAIPPERL
                     QKLQADYARDCVTLMQQASAAKLEAPELKDRRFSGDAWKASPAHAFAAAWYLLNARYLQ
                     ELADALETDPKTRERIRFAVQQWTAAAAPSNFLAFNPDAQKSILETQGESLRQGMMNLL
                     GDLQRGKISQTDESQFVVGKNLGCTEGAVVYENDLIQLIQYKPTTETVFERPLLIVPPC
                     INKFYILDLQPENSLVAHALSCGHQVFLVSWRNADASVAHKTWDDYMNEGLLAAIDAVQ
                     QVSGREQINTLGFCVGGTMLSTALAVLAARGEHPAASMTLLTAMLDFSDTGVVDVFVDR
                     AHVQMREQTIGGKSGTPPGLMRGVEFANTFSFLRPNDLVWNYVVDNYLKGRTPAPFDLL
                     YWNSDSTNLPGPMYAWYLRNTYLENRLREPGALTVCGEAVDLSRIDVPTFIYGSREDHI
                     VPWQTAYASTSILTGPLKFVLGASGHIAGVINPPAKKKRSFWVNDNDLPDAADDWFAGA
                     AEQPGSWWPTWTEWLGQYGGRKVAPPAQAGSAQFPVIEPAPGRYVLQRD"
     CDS             2444..3625
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1124:acetyl-CoA acetyltransferase (Score: 540.1;
                     E-value: 4.1e-164)"
                     /gene_kind="biosynthetic-additional"
                     /note="beta-ketothiolase"
                     /product="PhaA"
                     /protein_id="AAF23365.1"
                     /transl_table=11
                     /translation="MTDVVIVSAARTAVGKFGGALAKIAAPELGATVIRAVLERGGVKP
                     EQISEVILGQVLAAGSGQNPARQSLIKAGLPSAVPGMTINKVCGSGLKAVMLAANAIIA
                     GDADIVIAGGQENMSAAPHVLPGSRDGFRMGDAKLVDTMIVDGLWDVYNQYHMGITAEN
                     VAKEYGITREEQDAFAALSQNKAEAAQKAGRFDDEIVPVAIPQRKGEPLQFATDEFVRH
                     GVTAESLAGLKPAFAKDGTVTAANASGINDGAAAVLVMSAKKADALGLKPLARIKAYAN
                     AGVDPSVMGMCPVPASRRCLVRAVWTPGDLDLMEINEAFAAQALAVHKQMGWDTSKVNV
                     NGGAIAIGHPIGASGCRILVTLLHEMVKRDAKAGLASLCIGGGMGVALALERP"
     CDS             3738..4478
                     /NRPS_PKS="Domain: PKS_KR (7-168). E-value: 1.1e-15. Score:
                     49.8. Matches aSDomain: nrpspksdomains_AAF23366.1_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001:short-chain dehydrogenase/reductase SDR (Score:
                     214; E-value: 4e-65)"
                     /gene_kind="biosynthetic-additional"
                     /note="acetoacetyl-CoA reductase"
                     /product="PhaB"
                     /protein_id="AAF23366.1"
                     /transl_table=11
                     /translation="MSQRIAYVTGGMGGIGTSICQRLLKDGFTVVAGCGPNSPRRVKWL
                     EDQKALGFDFIASEGNVGDWDSTKEAFDKVKAEVGEIDVLVNNAGITRDVVFRKMTHED
                     WTAVIDTNLTSLFNVTKQVIDGMVERGWGRIINISSVNGQKGQFGQTNYSTAKAGIHGF
                     TMALAQEVATKGVTVNTVSPGYIGTDMVKAIRPDVLEKIVATIPVRRLGGPEEIDSIVA
                     WLASNDSGFATGADFSLNGGLHMG"
     CDS_motif       3759..3809
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AAF23366.1_0001"
                     /evalue="1.50E-05"
                     /label="PKSI-KR_m1"
                     /locus_tag="AAF23366.1"
                     /protein_end="24"
                     /protein_start="7"
                     /score="17.7"
                     /tool="antismash"
                     /translation="VTGGMGGIGTSICQRLL"
     aSDomain        3759..4241
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AAF23366.1_PKS_KR.1"
                     /evalue="1.10E-15"
                     /label="AAF23366.1_PKS_KR.1"
                     /locus_tag="AAF23366.1"
                     /protein_end="168"
                     /protein_start="7"
                     /score="49.8"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="VTGGMGGIGTSICQRLLKDGFTVVAGCGPNSPRRVKWLEDQKALG
                     FDFIASEGNVGDWDSTKEAFDKVKAEVGEIDVLVNNAGITRDVVFRKMTHEDWTAVIDT
                     NLTSLFNVTKQVIDGMVERGWGRIINISSVNGQKGQFGQTNYSTAKAGIHGFTMALA"
     CDS_motif       4149..4205
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AAF23366.1_0002"
                     /evalue="2.20E-03"
                     /label="PKSI-KR_m4"
                     /locus_tag="AAF23366.1"
                     /protein_end="156"
                     /protein_start="137"
                     /score="10.6"
                     /tool="antismash"
                     /translation="ISSVNGQKGQFGQTNYSTA"
     CDS             4599..5165
                     /codon_start=1
                     /inference="non-experimental evidence, no additional
                     details recorded"
                     /note="hypothetical protein similar to orf4 product of
                     polyhydroxyacids cluster from Cromatium vinosium, Tiocystis
                     violacea and Ralstonia eutropha"
                     /product="unknown"
                     /protein_id="AAF23367.1"
                     /transl_table=11
                     /translation="MTTTKKTAERLIKKYPNRRLYDTETSTYITLTDVKQLVLDQEDFK
                     VVDAKSSEDLTRTILLQIILEEESGGVPMFSSSMLSQIIRFYGHAMQGMMGTYLDKNIQ
                     AFIDIQNKLVDQSKNLYENNAMNPEIWSQFMNMQAPMMQGMMTSYIEQSKNMFVQMQEQ
                     MQNQAKSMFSSFPFKQPGSTEPEKK"
ORIGIN
        1 agcggtggcg gcgcatgcac ggtcaccgag cggtcgcgct tctatccgta ccggcgcgac
       61 cgcgtaaccg ggcatggcgg cggcgatctg gctgggcact gacgtcgcgc atgcatcgca
      121 tgctgccctg cagtatcggc ttatctcgat atttgcgcgg cagaatgccg atgtatgcgc
      181 aaccgaatga aaaaatgtga agtcaagcga atcaaaatgt tgatgtattc gccgggagcc
      241 ttgtcccgcc tgttgcgcgg ggattatctc cgtgcttgat ggatcgtcgt gatcctggtt
      301 tgacgcaacg cttcacatgg ggaaaacgat aatgataaaa ataccgcact gcggcagaat
      361 ctggagacgc ctctccagag catgacggcc ctgctgcacg gccggcacgc cgcgccgcga
      421 gcaggatttc cacgattgac gctcaggaat caccggtaag gcaggtaatg acagcatcga
      481 aaaactcgtc gacgtccgca caggcggaca ctttcgcggg ccagcaggca ttcgcccagg
      541 cagcccagca agcagcgcag ccgatgcagc agatgttcga ggcgtggttg agcgcgtggc
      601 ggggcttcgc ggatccggcg cgcgccgcga ccgcgtcggc cgcgacgaat ccgttcgcga
      661 cgttccagtt cccgaagtcg tttccgttcc agatgccgtc gatggccgac ttcggcggcg
      721 cggcatcgcc gttcgccggc ctgaagctgc cggccgccgc gatcccgccc gagcgcctgc
      781 agaagctgca ggccgactat gcgcgcgatt gcgtgacgct gatgcagcag gcgagcgccg
      841 cgaagctcga ggcgcctgag ctgaaggacc gccgcttcag cggcgacgcg tggaaggcat
      901 cgcccgcgca cgcgttcgcg gccgcgtggt acctgctcaa cgcgcgctac ctgcaggaac
      961 tcgccgacgc gctcgagacc gatccgaaga cgcgcgagcg gatccgtttc gcagtgcagc
     1021 aatggacggc cgccgccgcg ccgagcaatt ttctcgcgtt caacccggat gcgcagaagt
     1081 cgatcctcga gacccagggc gaaagcctgc ggcaggggat gatgaacctg ctgggcgacc
     1141 tgcagcgcgg caagatttcg cagaccgacg aatcgcagtt cgtggtcggc aagaacctcg
     1201 gctgcaccga gggcgcggtt gtctacgaga acgacctgat ccagctgatc cagtacaagc
     1261 cgacgacgga gacggtattc gagcggccgc tgctgatcgt gccgccgtgc atcaacaagt
     1321 tctacatcct cgacctgcag cccgagaact cgctcgtcgc gcacgcgctg tcgtgcggcc
     1381 accaggtgtt tctcgtgtcg tggcgcaacg cggatgcatc ggtcgcccac aagacctggg
     1441 acgactacat gaacgaaggc ctgctcgccg cgatcgacgc ggtgcagcag gtgagcggcc
     1501 gcgagcagat caacacgctg ggcttctgcg tcggcggcac gatgctctcc acggcgctcg
     1561 cggtgctggc cgcacgcggc gagcacccgg ccgcgtcgat gacgctgctc accgcgatgc
     1621 tcgacttctc cgacacgggc gtcgtcgacg tgttcgtcga cagggcgcac gtgcagatgc
     1681 gcgagcagac gatcggcggg aagagcggca cgccgccggg gctgatgcgc ggcgtcgagt
     1741 tcgcgaacac gttttcgttt ctgcgtccga acgatctcgt ctggaactac gtcgtcgaca
     1801 actacctgaa gggccgcacg ccggcgccgt tcgacctgct gtactggaac agcgattcga
     1861 cgaacctgcc gggcccgatg tacgcgtggt acctgcgcaa tacctatctc gagaaccggc
     1921 tgcgcgagcc gggcgcgctg accgtgtgcg gcgaggccgt cgacctgtcg cggatcgacg
     1981 tgccgacttt catctacggc tcgcgcgagg atcacatcgt gccgtggcag acggcctacg
     2041 cgtcgacgtc gatcctgacg ggcccgctga agttcgtgct cggcgcgtcg ggccacatcg
     2101 cgggcgtgat caatccgccg gcgaagaaga agcgcagctt ctgggtcaac gacaacgacc
     2161 tgcccgatgc cgcggacgac tggttcgccg gcgcggccga gcagcccggc agctggtggc
     2221 cgacctggac cgagtggctc ggccagtacg gtggccgcaa ggtggcgccg cccgcgcagg
     2281 ccggctccgc gcaattcccg gtgatcgagc cggcgcccgg tcgttacgtg ttgcagcgcg
     2341 attgacgaaa gactgacagc ggcgcacgct gtctggagcg ccgcagtatt tttaacaggg
     2401 cggggagcga tgcgccgtgt cgcgcctgcc cggagaaagg gaaatgacgg acgtagtgat
     2461 cgtatcggcc gcgcggaccg cggtcggcaa attcggcggc gctctcgcga agatcgcggc
     2521 gccggaactg ggcgccacgg tgatccgcgc ggtgctggag cgcggcggcg tgaagccgga
     2581 gcagatcagc gaagtgatcc tgggccaggt gctggcggcg ggctcgggcc agaacccggc
     2641 gcgccagtcg ctgatcaagg ccgggctgcc gagcgcggtg ccggggatga cgatcaacaa
     2701 ggtgtgcggc tcgggcctga aggcggtgat gctggcggcg aacgcgatca tcgccggcga
     2761 cgcggacatc gtgatcgcgg gcgggcagga gaacatgagc gcggcgccgc acgtgctgcc
     2821 gggctcgcgc gacgggttcc ggatgggcga cgcgaagctg gtcgacacga tgatcgtcga
     2881 cggcctgtgg gacgtctaca accagtacca catgggcatc acggcggaga acgtcgcgaa
     2941 ggaatacggg atcacgcgcg aagagcagga cgcgttcgcg gcgctgtcgc agaacaaggc
     3001 ggaagcggcg cagaaggccg ggcgtttcga cgacgagatc gtgccggtgg cgattccgca
     3061 gcgcaagggc gagccgctgc agttcgcgac ggacgagttc gtgcgccacg gcgtgacggc
     3121 ggagtcgctg gcggggctga agccggcgtt cgcgaaggac ggcaccgtga cggcggcgaa
     3181 cgcatcgggg atcaacgacg gcgcggcggc ggtgctggtg atgtcggcga agaaggccga
     3241 tgcgctgggc ctgaagccgc tcgcgcggat caaggcgtac gcgaacgcgg gcgtggatcc
     3301 gagcgtgatg gggatgtgcc ctgtgccggc gtcgcggcgg tgcctggtac gcgcagtctg
     3361 gacgccgggc gacctggacc tgatggagat caacgaggcg ttcgcggcgc aggcgctggc
     3421 ggtgcacaag cagatgggct gggacacgtc gaaggtgaac gtgaacggcg gggcgatcgc
     3481 gatcggccat ccgatcgggg cgtcgggctg ccggatcctg gtgacgctgc tgcacgagat
     3541 ggtcaagcgc gatgcgaagg cggggctggc gtcgctgtgc atcggcggcg gaatgggcgt
     3601 cgcgctcgcg ctcgagcgtc cgtaatcgtc tctcaaggcg tctgccgggc agtatgcgcg
     3661 caggcgccgc gtgtagtgcg tgtagtcaga gggcgccgct cggcaactct cgaaaccaag
     3721 aaaatggagt gagatttatg tctcagcgaa ttgcttacgt aacgggcggt atgggtggca
     3781 tcggcaccag catctgccag cgcctgctga aggacggctt cacggtggtc gcaggctgcg
     3841 gcccgaactc gccgcgccgc gtgaagtggc tcgaggacca gaaggcgctc gggttcgact
     3901 tcatcgcatc ggaaggcaac gtcggcgact gggactcgac caaggaagcg ttcgacaagg
     3961 tcaaggccga ggtcggcgag atcgacgtgc tggtcaacaa tgcgggcatc acgcgcgacg
     4021 tcgtgttccg caagatgacg cacgaggact ggacggccgt catcgacacc aacctgacga
     4081 gcctgttcaa cgtgacgaaa caggtgatcg acgggatggt cgagcgcggc tggggccgga
     4141 tcatcaacat ctcgtcggtg aacggccaga agggccagtt cggccagacc aactattcga
     4201 ccgcgaaggc cggcattcac ggcttcacga tggcgctcgc gcaggaagtc gcgacgaagg
     4261 gcgtgacggt caacaccgtg tcgccgggct acatcggcac cgacatggtc aaggcgatcc
     4321 gccctgacgt gctcgagaag atcgtcgcca cgattccggt gcgtcgtctc ggcgggcccg
     4381 aggagatcga ctcgatcgtc gcgtggctcg cgtcgaacga ttcgggtttt gcgacgggcg
     4441 ccgacttctc gctgaacggc ggcctgcaca tgggctgagc ccgcgaaccg cgcgggccgg
     4501 gtggccgcgc gttgcgtcgt ttcccaaggg gccccgccac ccgtctttcg ggcggcgggg
     4561 atttgtcact gcgctacgct ggcacttaaa ggcgttacat gactactaca aagaaaacgg
     4621 ccgaacggct catcaagaag tatccgaacc gccggctcta cgataccgag acaagcacgt
     4681 acatcacgct caccgatgta aagcagctcg tgctggacca ggaggacttc aaggtcgtcg
     4741 atgcgaaatc gagcgaagac ctgacgcgca cgatccttct tcagatcatt ctcgaagagg
     4801 aaagcggcgg cgtgccgatg ttctcgtcgt cgatgctgtc gcagatcatc cgcttctacg
     4861 ggcatgcgat gcaggggatg atgggcacct acctggataa gaacatccag gcgttcatcg
     4921 acatccagaa caagctcgtc gaccagtcga agaacctgta cgagaacaat gcgatgaatc
     4981 cggagatctg gtcgcagttc atgaacatgc aggcgccgat gatgcagggg atgatgacga
     5041 gctatatcga gcaatcgaag aacatgttcg tgcagatgca ggagcagatg cagaatcagg
     5101 cgaagtcgat gttcagctcg ttcccgttca agcagccggg atcgacggag ccggagaaga
     5161 agtaagcggc gtttcggtgc ggacgcgcaa ggcgccgcgc cggcggtatc gcccgcgcgt
     5221 gctcgggcga tgcaatgttg cagtgcatca gtgaaaatta aaacagagtg ggccggcggc
     5281 aaatgccggt cggccgaata tcagataaag cgctgattgc aaaagatgcc gatggccgga
     5341 tttcccggcc gggcttaatc ggaatcgtcg aagcggcttg ccgccgatcc gttcgcgcag
     5401 ttatttccgt aaatgaacag gtttctgttt tcggaaaatg ccgaatagat tgatcggaaa
     5461 aacttccttg gcactgaacg agtaaatgcc gtcgctgatg
//
