LOCUS       BGC0000867              6420 bp    DNA     linear   BCT 10-JUN-2004
DEFINITION  Ectothiorhodospira shaposhnikovii polyhydroxyalkanoic acid
            biosynthetic gene cluster, complete sequence.
ACCESSION   BGC0000867
VERSION     BGC0000867
KEYWORDS    .
SOURCE      Ectothiorhodospira shaposhnikovii
  ORGANISM  Ectothiorhodospira shaposhnikovii
            Bacteria; Proteobacteria; Gammaproteobacteria; Chromatiales;
            Ectothiorhodospiraceae; Ectothiorhodospira.
COMMENT     ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2022-11-07 20:52:54
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6420
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..6420
                     /db_xref="ATCC:31751"
                     /db_xref="taxon:1054"
                     /mol_type="genomic DNA"
                     /organism="Ectothiorhodospira shaposhnikovii"
                     /strain="ATCC 31751"
     subregion       1..6420
                     /aStool="mibig"
                     /contig_edge="True"
                     /label="Other"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..6420
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            complement(22..648)
                     /gene="phaB"
     CDS             complement(22..648)
                     /NRPS_PKS="Domain: PKS_KR (1-126). E-value: 6.8e-17. Score:
                     53.8. Matches aSDomain: nrpspksdomains_phaB_PKS_KR.1"
                     /codon_start=1
                     /gene="phaB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001:short-chain dehydrogenase/reductase SDR (Score:
                     181.4; E-value: 3.6e-55)"
                     /gene_kind="biosynthetic-additional"
                     /note="PhaB"
                     /product="NADPH-dependent acetoacetyl CoA reductase"
                     /protein_id="AAG30255.1"
                     /sec_met_domain="adh_short (E-value: 1.1e-34, bitscore:
                     108.9, seeds: 230, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKAEGLDMHIAAGDVSSFQSCADMIQDIESRLGPVDILVNCAGIT
                     KDKTLRKMTEDQWRDVININLNSVFNVTRHVVEGMTSRGFGRIVNISSVNGQKGQFGQA
                     NYSAAKAGMHGFTMAVAQETAAKGVTVNTVSPGYTATAMTDAMPEDVRNAIVAQIPAGR
                     MGSTADIAFAVAMLCDEEAATSPVPTFRSMVVCLPPSEAVVRPIG"
     aSDomain        complement(271..645)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_phaB_PKS_KR.1"
                     /evalue="6.80E-17"
                     /label="phaB_PKS_KR.1"
                     /locus_tag="phaB"
                     /protein_end="126"
                     /protein_start="1"
                     /score="53.8"
                     /tool="antismash"
                     /translation="KAEGLDMHIAAGDVSSFQSCADMIQDIESRLGPVDILVNCAGITK
                     DKTLRKMTEDQWRDVININLNSVFNVTRHVVEGMTSRGFGRIVNISSVNGQKGQFGQAN
                     YSAAKAGMHGFTMAVAQETAA"
     CDS_motif       complement(322..381)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_phaB_0001"
                     /evalue="3.50E-05"
                     /label="PKSI-KR_m4"
                     /locus_tag="phaB"
                     /protein_end="109"
                     /protein_start="89"
                     /score="16.3"
                     /tool="antismash"
                     /translation="NISSVNGQKGQFGQANYSAA"
     gene            complement(844..1215)
                     /gene="phaP"
     CDS             complement(844..1215)
                     /codon_start=1
                     /gene="phaP"
                     /note="PhaP"
                     /product="phasin"
                     /protein_id="AAG30256.1"
                     /transl_table=11
                     /translation="MNDMIELFNKANQTGFDTFRKLSDINQQTFQKLVEQQLDLTTGLV
                     DVSMKHLEQVGKAKGYQELVTLQTDLLRDCSQKMVATYKSGQEILDDARNSMSRLMDDS
                     VKAAEETVKHVSTVAKKAA"
     CDS             complement(1371..1715)
                     /codon_start=1
                     /note="ORF3"
                     /product="unknown"
                     /protein_id="AAG30257.1"
                     /transl_table=11
                     /translation="MLVRFIRFYDEAVHEAFSGYLDQSLRFFADQQRDFNDRMRDVLGG
                     RATWDITEMTRRNLEFWKGMQDQFFKAAVFPGPGHETRKDPAKPPEQSPPADSGDGGTA
                     TADKAGKKPK"
     gene            complement(2018..3202)
                     /gene="phaA"
     CDS             complement(2018..3202)
                     /codon_start=1
                     /gene="phaA"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1124:acetyl-CoA acetyltransferase (Score: 542.3;
                     E-value: 9.2e-165)"
                     /gene_kind="biosynthetic-additional"
                     /note="PhaA"
                     /product="beta-ketothiolase"
                     /protein_id="AAG30258.1"
                     /transl_table=11
                     /translation="MAESIVIVDAARTPVGTFNGSLSQIPASTLGAMVMKALLARTGLS
                     PEQVDEVILGQVLQAGVGQNAARQAAVKAGIPYSSPAMTINKVCGSGLKAVHLAWQSVL
                     CGDSNVVIAGGQENMSASPHVLPGSRQGTKMGDWKMVDTMIKDGLWCAFNDYHMGTTAE
                     NIAGEFRISRDEQDAFAAQSQQRAEAAQKAGYFKDEIVPVEIPQKKGEVKVFDMDEFPR
                     HGTTAEGLGKLRPAFSRDGTVTAGNASGINDGAAAVVVMRESMASELGLNPLARIVSFA
                     AAGVDPAIMGTGPIPATIKCLEKAGWRPADLDMVEANEAFAAQAIAVNKQLGWDLSKVN
                     VSGGAIAIGHPIGASGARVLVTLLHGMKRTGARRGLATLCIGGGQGVALAVEAV"
     CDS             2153..3136
                     /codon_start=1
                     /note="ORF7"
                     /product="unknown"
                     /protein_id="AAG30261.1"
                     /transl_table=11
                     /translation="MADGNGPAAHVHLGQVPAQLLVDCNGLGGKGLVGLHHVQIGGTPA
                     GLLQALDGGGDGTGAHDRRVHAGGGEGNDAGQGVEAQLAGHGFTHDHHGRGTVIDTGGI
                     AGGHRAIPAESRPQLAQAFRRGAVTGKFIHVEDLDLALFLRNLHRHDLILEISRLLGGL
                     GPLLGLGGKGVLLIPANPELAGDVLGGGAHVIIIEGTPQAILDHGIHHLPVAHLGSLTG
                     ARQDMGRGTHVFLASGDHHVGIAAQDRLPGQMNRLETTAADLIDGHGRGRIRDTGLHGG
                     LTGGILAHAGLQHLPEDDLVHLLGGQAGASQQRLHDHGPQGRGRNL"
     gene            3624..4739
                     /gene="phaE"
     CDS             3624..4739
                     /codon_start=1
                     /gene="phaE"
                     /product="PHA synthase subunit PhaE"
                     /protein_id="AAG30260.1"
                     /transl_table=11
                     /translation="MADSNPFNPEDWMEAQRKYWSAWMDMSRKAVGGSEGTNHWGDGLD
                     QWWKTVSSGMPLSGNDAMDQMMKIGRNYFSMAENLYRGAEGNNAAEAVNKWLDQMSNAF
                     QGFTNMPGASRDANAFWQMPLDTWRRATSSFMPMPGDYMQALRPPARAPYGDAVQEQLD
                     RFLSVPGVGYTRESQEQYQNLAKLMVNYQQAMQNYQAAFNKVGMDSIDRFRARLDDAGR
                     QDKPITSMRALYDEWVDVCEEVYGEFVMSEEYAKIYGEMVNSLMAVKRQGTRLMDEVTE
                     SLNMPTRREVNTLHRRLQESRRELQALKATVRKLTDQVNGQPVPASVEAQVESKAEDKG
                     NASASRAATGTRSRATATKRQTTTTKAKQND"
     gene            4755..5822
                     /gene="phaC"
     CDS             4755..5822
                     /codon_start=1
                     /gene="phaC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /product="PHA synthase subunit PhaC"
                     /protein_id="AAG30259.1"
                     /sec_met_domain="PF00561 (E-value: 1.3e-30, bitscore: 95.7,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIPIQIRPDQVMDELTTFQNKLTQGLQNLADAGDIPSGVTPKEAV
                     YKEDKLTLYRFQAPASVVQHPVPVLIVYALVNRPYMTDLQDDRSTVKGLLEAGMDVYLI
                     DWGYPDRADRFLTMDDYLNGYLDRCVDEICRRHNLDKINILGICQGGTFSLCYSAMHPE
                     KVQNLITMVTPVDFQTPDNILSHWVKHVDIDTLVDTMGNVPGELLNWTFLNLKPYQLMG
                     QKYLDMVEVLQDKDKLDNFLRMEKWIFDSPDQVGETYRQFIKDFYQKNKLMNGGLQIGE
                     YEVSLANVTMPVLNIFAEQDHLVPPDASKALAGKVGTKDYTELSFPGGHIGIYVSGKAQ
                     KTVPPAIGKWVKERG"
ORIGIN
        1 gggcccgcac gagacgtggt ctcagccgat agggcgaaca acagcctcag aaggaggtaa
       61 acagaccacc attgaccgga atgttggcac cggtgatgta gccgcctctt cgtcgcagag
      121 catggccacg gcgaaggcga tgtcggccgt ggagcccatg cgcccggccg gaatctgcgc
      181 cacgatggcg ttgcgcacat cctcgggcat ggcgtcggtc atggccgtgg cggtatagcc
      241 gggggacacg gtgttgacgg tgacaccctt ggcggccgtc tcctgggcca ccgccatggt
      301 gaagccgtgc ataccggcct tggccgcgga atagttggcc tgaccgaact gacccttctg
      361 accgttgacg gaggagatgt tgacgatgcg gccgaaaccg cgggaagtca ttccctccac
      421 cacgtgacgg gtgacgttga acacgctgtt cagattgatg ttgatgacat cacgccactg
      481 gtcttcggtc atcttgcgca gcgtcttgtc cttggtgatg ccggcgcagt tgaccaggat
      541 gtcgacgggt cccaggcggg actcgatgtc ctggatcatg tcggcacagg actggaacga
      601 ggacacatct ccggcggcga tgtgcatgtc caggccttcc gccttcatct gctgctgcca
      661 ggcctcggcc tcggcctgca gggcgggcag gtaattggcc acgaccacat gaccctgacg
      721 ggccagcttc ttgcagatct cggtgccgat accgccggta ccgccggtca ctaacgctac
      781 tcgcttggtc actattcgga ctccttgttt cttgactggc cttgagggct gcgacagagg
      841 aaatcaggca gccttcttgg caacagtgct cacgtgcttg acggtttctt ccgccgcctt
      901 gacggaatca tccatcaacc ggctcatgga attgcgggca tcatcaagga tctcctgccc
      961 gctcttgtaa gtggcaacca tcttctggct gcaatcacgc agcagatccg tctgcagggt
     1021 cacgagttcc tgatacccct tggccttgcc cacctgctca aggtgtttca tgctcacatc
     1081 caccaggccc gtggtcaagt ccagctgctg ctccaccagc ttctggaagg tctgctggtt
     1141 gatgtcgctc aacttgcgga aggtgtcgaa gccggtctgg ttggccttgt tgaacagctc
     1201 gatcatgtcg ttcatggtgc gctctccggg ttggggttat gtggacacag catccagcct
     1261 tgccgccgcc tatgctgcag cgcaacatat aaccccatcc gggcacggcg tcaagccgat
     1321 atgcgggttt tgcgacgcat acccgcccgc caccaagcgc ggacagtcga ctatttcggt
     1381 ttttttcccg ccttgtccgc ggtggcggtg cccccatcac ccgaatccgc cggcggcgac
     1441 tgttccggcg gcttggcggg atccttcctg gtctcatgac ccgggccggg gaataccgcg
     1501 gccttgaaga actgatcctg catgcctttc cagaactcca ggttgcgacg ggtcatctcg
     1561 gtaatgtccc aggtagcccg cccacccagc acgtcccgca tgcggtcatt gaaatcccgc
     1621 tgctgatcgg cgaagaatcg caggctctgg tccagataac cggaaaaagc ctcatgaacc
     1681 gcctcgtcat agaagcggat gaaacgcacc agcatctcgg tggtgaagag cggctctccg
     1741 gccgtctcct gctcggtgat gatctgaatc agaatgcccc gggtgatgtc cttgcccgac
     1801 tgactgtcga tcaccttcac gttgatcccg cggcgcacca gctgctgtac atcggccagc
     1861 gtcacataac ggctctcctc ggtatcgtag agacgccgat tgggatactt cttcactatc
     1921 cgcggttcgt tcacccactt ctcccatcaa cattcaactg gaccgcttgc aaagggcgga
     1981 tccgaaaaga aacccgaatc cgcccgtcac gccccggtca gactgcctcg acagccagcg
     2041 ccacaccctg accaccaccg atgcacaggg tggccagacc ccgcctggca ccggtgcgct
     2101 tcatgccgtg caggagggtc accagcaccc gggcgccgga ggcaccgatg ggatggccga
     2161 tggcaatggc cccgccgctc acgttcacct tggacaggtc ccagcccagc tgcttgttga
     2221 ctgcaatggc ctgggcggca aaggcctcgt tggcctccac catgtccaga tcggcgggac
     2281 gccagccggc cttctccagg cacttgatgg tggcggggat gggaccggtg cccatgatcg
     2341 ccgggtccac gccggcggcg gcgaaggaaa cgatgcgggc cagggggttg aggcccagct
     2401 cgctggccat ggattcacgc atgaccacca cggccgcggc accgtcattg ataccggagg
     2461 cattgccggc ggtcaccgtg ccatcccggc tgaaagccgg cctcagcttg cccaggcctt
     2521 ccgccgtggt gccgtgacgg ggaaattcat ccatgtcgaa gaccttgacc tcgccctttt
     2581 tctgcggaat ctccaccggc acgatctcat ccttgaaata tcccgccttc tgggcggcct
     2641 cggcccgctg ctgggactgg gcggcaaagg cgtcctgctc atcccggcta atcctgaact
     2701 cgccggcgat gttctcggcg gtggtgccca tgtgataatc attgaaggca caccacaggc
     2761 catccttgat catggtatcc accatcttcc agtcgcccat cttggttccc tgacgggagc
     2821 ccggcaggac atggggagag gcactcatgt tttcctggcc tccggcgatc accacgttgg
     2881 aatcgccgca caggaccgac tgccaggcca gatgaaccgc cttgagacca ctgccgcaga
     2941 ccttattgat ggtcatggcc ggggaagaat aagggatacc ggccttcacg gcggcctgac
     3001 gggcggcatt ctggcccacg ccggcctgca gcacctgccc gaggatgacc tcgtccacct
     3061 gctcggggga caggccggtg cgagccagca gcgccttcat gaccatggcc cccagggtcg
     3121 aggccggaat ctgtgacagg gaaccgttga aggtaccgac cggggtgcgc gccgcatcga
     3181 cgatgacaat agattcagcc atgtaaatcc tcctgatcag gggcgtgttt ctgaaatggg
     3241 gtatgcctgc ggccacccct tgaccgggtt ttcatgccgg gcaccggggg tggttcgcac
     3301 tttcagggta cttggtcttg ctttcctggc atatttgatc caaaaggaat ggcacccttg
     3361 aggatcaagt gattgctccg gaagtttata ccagaagcgc tgcacccctg accaaccatg
     3421 gagcatcatc ccggcagaat tgttgcagcg cacaaaatct atgctaacct tatccttata
     3481 atgctgtacc ctgtcagatc gtcccaagca atgaccgccc aggcccggga atgcccgtcg
     3541 gcccaggctc ttgattgagc ggtcgcgaca acgatcccgg ctgataacca cctggcccag
     3601 aatggaccca aaggagatga ttgatggctg actcaaaccc cttcaacccc gaagactgga
     3661 tggaagccca gcgcaaatac tggtctgcct ggatggacat gagccgcaag gccgtaggcg
     3721 gcagcgaagg caccaaccac tggggggatg gcctggatca gtggtggaag accgtttcca
     3781 gcggcatgcc cctgtccggc aatgacgcca tggaccagat gatgaagatc ggtcgcaatt
     3841 atttttccat ggcggaaaac ctctatcggg gcgcggaagg caacaatgcc gccgaggccg
     3901 tgaacaagtg gctcgaccag atgagcaatg ccttccaggg cttcaccaac atgcccggtg
     3961 ccagccggga tgccaatgcc ttctggcaga tgcccctgga cacctggcgt cgcgccacgt
     4021 cgtccttcat gcccatgccg ggcgactaca tgcaggccct gcggccgccg gcccgtgccc
     4081 cctacggcga tgccgtgcag gaacagctgg accgtttcct gtccgtgccc ggcgtcggtt
     4141 acacccgcga atcccaggag cagtaccaga acctggccaa gctgatggtc aattaccagc
     4201 aggccatgca gaactatcag gccgccttca acaaggtcgg catggactcc atcgaccgct
     4261 tccgcgcccg tctggacgac gccggtcgtc aggacaagcc catcacctcc atgcgcgccc
     4321 tctacgatga gtgggtggac gtctgcgaag aggtctacgg cgaattcgtc atgtccgagg
     4381 agtacgccaa gatctacggc gagatggtca acagcctgat ggcggtaaag cgccagggca
     4441 cccgcctcat ggacgaggtc accgaaagcc tgaacatgcc cacccgccgc gaagtgaaca
     4501 ccctgcaccg ccgcctgcag gaaagccgcc gtgaactgca ggccctcaag gccacggttc
     4561 gcaagctgac cgatcaggtg aacggccagc ccgtgccggc aagcgtggaa gcccaggtgg
     4621 aaagcaaggc cgaagacaag gggaacgcca gcgcctcccg cgccgccacc ggcacccgta
     4681 gccgcgccac ggccacgaaa cgccagacca ccaccaccaa ggccaagcag aacgactaaa
     4741 aggagagccg gccaatgatt ccgattcaga tcagacccga tcaggtcatg gacgaactga
     4801 ccaccttcca gaacaagctc acccagggcc tgcagaacct ggccgatgcc ggtgacattc
     4861 cctccggcgt gacacccaag gaagcggtct acaaggaaga caagctcacc ctgtaccgct
     4921 tccaggcacc ggcctccgtg gtccagcacc cggtaccggt gctgatcgtc tacgccctgg
     4981 tgaaccgccc ctacatgacg gaccttcagg atgaccgctc cacggtcaag ggcctgctgg
     5041 aagccggcat ggatgtttac ctgatcgact ggggctaccc tgaccgggcc gaccgtttcc
     5101 tgaccatgga cgattatctc aacggctacc tggaccgctg cgtggacgag atctgccgtc
     5161 gccacaacct ggacaagatc aacatcctgg gtatctgcca gggcggcacc ttcagcctgt
     5221 gctacagcgc catgcatccg gaaaaggtgc agaacctgat caccatggtg accccggtgg
     5281 acttccagac cccggacaac atcctctccc actgggtcaa gcacgtggac atcgacaccc
     5341 tggtggacac catgggcaat gtcccgggtg aactgctcaa ctggaccttc ctcaacctca
     5401 agccctatca gctcatgggc cagaagtatc tggacatggt ggaagtgctg caggacaagg
     5461 acaagctgga caacttcctg cgcatggaga agtggatctt cgacagcccg gaccaggtcg
     5521 gcgaaaccta ccgccagttc atcaaggact tctaccagaa gaacaagctc atgaacggcg
     5581 gccttcagat cggcgagtac gaagtgagcc tggccaacgt gaccatgccg gtactgaaca
     5641 tctttgccga gcaggatcac ctggtaccgc cggatgcctc caaggccctg gccggcaagg
     5701 tgggcaccaa ggactacacc gaactgtcct tccccggcgg ccatatcggc atctatgtga
     5761 gcggcaaggc acaaaagacc gtaccgccag ccatcggcaa gtgggtcaag gaacggggtt
     5821 aatcaatccc ctgtcatccc tctccccctc ccccaccggg gggaggaggg agcggcgcca
     5881 cccggcccgt gcgccggaat tccagatagt catccagtat ccggcgatga tcgaaggcca
     5941 ggggccggtc cacttggtcc gcggccacaa cccagaccgc cttggcatca tccgccgcca
     6001 ccggcatccc ccgggcaacc gccgcataga cggcactcac cgtatgcccc cgttcatccc
     6061 ggccgggatc cgaatagcag cccagcagga cctccagtcg caccaccaat ccggtttcct
     6121 cttgggcctc gcggatcgcc gcctgctcca gtgactcccc cacatccatg aagccgccgg
     6181 gcaaggccca gccatggggc ggattgaggc gctcgatcag cacgatacga tcggcctgat
     6241 cctccaggcg gatgatcaca tcggcggcga tgagcggcgt cacgggtctg ggcacgggac
     6301 tactccttgc cgataaagcg gcggatatga cgccggtcac ggcggtccgg gcgatgttcg
     6361 gtatggggca ccgccaggcg ggccagacgc cgtgttcggc ctcctcggcg gacggggccc
//
