LOCUS       BGC0000879             14617 bp    DNA     linear   BCT 08-OCT-2008
DEFINITION  Streptomyces rimosus toyocamycin/sangivamycin biosynthesis gene
            cluster, complete sequence.
ACCESSION   BGC0000879
VERSION     BGC0000879
KEYWORDS    .
SOURCE      Streptomyces rimosus
  ORGANISM  Streptomyces rimosus
            Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
            Streptomyces.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-07 20:55:49
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 14617
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..14617
                     /culture_collection="ATCC:14673"
                     /db_xref="taxon:1927"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces rimosus"
     protocluster    1..14617
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[4312:4708]"
                     /cutoff="20000"
                     /detection_rule="(LipM or LipV or LipU or ToyB or TunD or
                     pur6 or pur10 or nikJ or nikO or truD)"
                     /neighbourhood="10000"
                     /product="nucleoside"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      4313..4708
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(LipM or LipV or LipU or ToyB or TunD or
                     pur6 or pur10 or nikJ or nikO or truD)"
                     /neighbourhood="10000"
                     /product="nucleoside"
                     /protocluster_number="1"
     cand_cluster    1..14617
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(LipM or LipV or LipU or ToyB or TunD or
                     pur6 or pur10 or nikJ or nikO or truD)"
                     /kind="single"
                     /product="nucleoside"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..14617
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000879"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..14617
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="nucleoside"
                     /region_number="1"
                     /rules="(LipM or LipV or LipU or ToyB or TunD or pur6 or
                     pur10 or nikJ or nikO or truD)"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            1168..4053
                     /gene="toyA"
     CDS             1168..4053
                     /codon_start=1
                     /gene="toyA"
                     /gene_functions="regulatory (smcogs) SMCOG1149:LuxR family
                     transcriptional regulator (Score: 482.1; E-value:
                     6.3e-146)"
                     /gene_kind="regulatory"
                     /note="similar to LuxR"
                     /product="ToyA"
                     /protein_id="ACF06633.1"
                     /transl_table=11
                     /translation="MSVATPTAEDTYRAKDSAVLAPRPEMRVLRQALADSGAGRGGHWV
                     VYGETGAGKTSVLRALREEARVLGHAAVELKCTPMEGCVPFATARKLVALFGASLPARG
                     PAAEALRRAGRALSGRNGSAAGRGSGLGVVLTGLQEAAQALTAAAPLLITVDDIGSADA
                     ASLDCFSYLLNQCEGVHRLLIVATLADGEPVQAPAALEGLRLGARGRIDLGGLDLPGTA
                     ELVAAVTGRPCDEGLAAACRTATAGNPLFLRALARCLPARRPVPAHEIAGLGPRAVADH
                     VLARLARISEPASAMARTVAVLGDDTDPAMVAELANLDCSTGLEAADVLVRTRLFHDGG
                     QMSFRHPVVAAALREQTSIVVKNTLHMQAAEHLLSRDAPAEQVAGHLMATTLPLTSSWM
                     VDALRSAARSALVREETGTAVGYLQRAVQDARGVDWRRAVAQLADAQLLTDPSAAIGRL
                     AELARQGVTAEEHPQLLARLADASFFFATRPRIRHQLDEAVEAVAVSQPHYTADTQLYR
                     ALHALSELPTKEACELGDRYLASEPREGVLRSAAGALSAVSAYAAGRGRKAAVDRVKEA
                     LRDPDLYELPNTAPLCAGTALLAHEGDLDLASEYARHGIAVARREGRQLQVAALLPTLA
                     KIAQVRGDLREAENLLRTCLKTFARYGVWRANPAALWAGSVLVGVLVDLGEPAAARTVL
                     SESGCHGDLPAMWSTQAMLEQRARVRMAAGELTDALEDLRECGRRSAALGLNSVEFGGW
                     RRLAAEVLHQLGRPDEARRLAEEELAAVRGQATDRTQGAALRTAARVIGGDAGLGPARE
                     AVRILAGTPDVLELARAQLTLGTLLGGTGDRTAAQGTLAQAHALASRCGAAPVEEQARE
                     QLHALGRQHGGRAVTGLLSLTRRERQVLADAVRGRSNGAIADALFITRRTVEIHLTSAY
                     RKLGIQGRKEFAQLYNRQELWALLTEETAGR"
     misc_feature    1168..12985
                     /note="toyocamycin/sangivamycin biosynthesis gene cluster"
     misc_feature    1252..1254
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            4313..4708
                     /gene="toyB"
     CDS             4313..4708
                     /codon_start=1
                     /gene="toyB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     nucleoside: ToyB"
                     /gene_kind="biosynthetic"
                     /note="similar to 6-pyruvoyltetrahydropterin synthase"
                     /product="ToyB"
                     /protein_id="ACF06634.1"
                     /sec_met_domain="ToyB (E-value: 2.7e-73, bitscore: 233.7,
                     seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFPFTRRASPLGFRISKEFHFSASHRLDHLDEGHPCSRMHGHNYV
                     VELELSAGDDALTKAGFVRDYGELAAFKQWLDAELDHRHLNDVVDGLNPSAENLARWIH
                     ERWRTGFPELTAVRVSETDKTWAEYRA"
     misc_feature    4778..5479
                     /gene="toyC"
                     /note="similar to ToyC; similar to radical SAM"
     gene            4778..5479
                     /gene="toyC"
     gene            5476..6078
                     /gene="toyD"
     CDS             5476..6078
                     /codon_start=1
                     /gene="toyD"
                     /note="GTP cyclohydrolase I"
                     /product="ToyD"
                     /protein_id="ACF06635.1"
                     /transl_table=11
                     /translation="MTTSAETRTLELPREHAEPGTDPLETLARQLLTEIGEDPDRSGLR
                     DTPARFARWWREFTQYDPGQVETVFPLHTVGQLVTVSDIHVWSLCEHHLLPFSCTLTIA
                     YRPASHVLGLSKFARIAHRHAHRLQIQERLVHDIASEITAVTGSPDVAVIGKGEHLCMS
                     MRGIRTPAVMTSSAFTGIFEDFGPARDEVVSAAGGPR"
     gene            6165..7319
                     /gene="toyE"
     CDS             6165..7319
                     /codon_start=1
                     /gene="toyE"
                     /note="similar to GMP reductase"
                     /product="ToyE"
                     /protein_id="ACF06636.1"
                     /transl_table=11
                     /translation="MKPLREIPTGLGLDDVLLVPQRTSVTSRSHTDVSTELVPGLRLSV
                     PILSANTPWCTGARMAAAMALAGGLGVIHRMQTAEDQAAEVTAVKKEHPKEESAAPGAT
                     VDERGRLRAAAAVGVTDDYLDRAALLVEAGADALVVDVAHGHADYVLKAVEQLKSRWPG
                     VPLVGGNVATPAGTRDLIDAGADAVKVGIGPGGICTTRLVAGSGMPQFTAVLECAEEAA
                     GRGVPVIADGGIKEPGDVAKVLAAGARTAMLGSALAGAEESAALLVEHDGRPAKVSTGF
                     VTFGMQLTLKRARGEAVTKEELAQYTPEGVEATWPFSGPAAGTVNRFVGGLRSGMSYSG
                     AHTLAEFRERACFVRVTPAGLAENHPHALGRAQQVPLDYAAEAV"
     gene            7316..8671
                     /gene="toyF"
     CDS             7316..8671
                     /codon_start=1
                     /gene="toyF"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1063:argininosuccinate lyase/adenylosuccinate lyase
                     (Score: 156.4; E-value: 2.1e-47)"
                     /gene_kind="biosynthetic-additional"
                     /note="similar to adenylosuccinate lyase"
                     /product="ToyF"
                     /protein_id="ACF06637.1"
                     /transl_table=11
                     /translation="MTTPIDTGRYGSPRMRALFTDRARYEAFVRVEGALATAQGRYGVI
                     PREAAEAIAEQARTHRCSLERVAALEETSRHELFAAVQEFAESCGAHGAWVHYGATSSD
                     ILDTALALQLTEAIALLEEDLRDLLTALLARIDAVGDHVMIGRTHGQQAQPVTLGFKLA
                     IVLDRFVRCLERLAQAKERVVMGKIAGAVGSLAGIGAHGPEVQRETLRELGLPEPAVCA
                     QAVARDRIAEFFCWAGLTAACLEDLATEVRNLQRTEIGEVAEPFAEGGQIGSSAMPHKR
                     NPVQSERICSLARLVRSLVGPALENVTSWHERDLANSANERFTLPEACVLLDEMLTTAT
                     YVIDGLRVFPDRMRHNLEHSHNEHLSEAVLLLLVQRGMDRLAAYRLLQEATGRATGEDG
                     DMVDALLAHPRLAGRLDAEEVRRLAAAGADVGHCRDLTAAAVKRAEHIVQGG"
     gene            8673..9728
                     /gene="toyG"
     CDS             8673..9728
                     /codon_start=1
                     /gene="toyG"
                     /note="similar to adenylosuccinate synthetase"
                     /product="ToyG"
                     /protein_id="ACF06638.1"
                     /transl_table=11
                     /translation="MPCTIIVGGQWGDEAKGKMASYLALKDGVDLAVRAGLGPGAGHTI
                     VHGGRTHKLRQVPSAVVNERTRLLLGAGVLVNPEVLLREIDELGVADRIGVDFRATVID
                     PGHVEAEQRERNLTDTVRSTGSGHGPALAARALRNARRAMDVPELAPYLADVAVEVNGA
                     VDAGAQVQVEGTNGFGLSVLYGTYPHTVGKDSTASTAAADAGLGPLAVDDVVLVFRAYP
                     TRVGAGAFPTELPEQEAERIGVEYGTVTGRRRRMGRFDLEQAREAVRINRPSRIAVSFL
                     DYLAPEVRGQQEGPLPPGAAAGLVEEIERELGVRVELLGTGPGTDEVIDLRPAPAGAGT
                     TAPLLRTRSTA"
     gene            9725..10255
                     /gene="toyH"
     CDS             9725..10255
                     /codon_start=1
                     /gene="toyH"
                     /note="similar to phosphoribosylpyrophospate transferase"
                     /product="ToyH"
                     /protein_id="ACF06639.1"
                     /transl_table=11
                     /translation="MTAGPSLWKRQRLLTMSWQTLGTLLTGLAEEIRAAGPLPDTVVGI
                     TRGGLTPAVALAHRLDVPEFRIMGIPRNSSNSRYSERGAAALEYVVPDTPLTGKRVLVV
                     DDIMGDGGTMETAVGTLRALDAAEIRTAVLVRNQNCAGRPDHQGVEVDDWTVFPWEAPP
                     GPAEPTVPLEAVR"
     gene            10252..10977
                     /gene="toyI"
     CDS             10252..10977
                     /codon_start=1
                     /gene="toyI"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1115:HAD-superfamily hydrolase, subfamily IA, variant
                     (Score: 60.6; E-value: 2.9e-18)"
                     /gene_kind="biosynthetic-additional"
                     /note="HAD superfamily protein"
                     /product="ToyI"
                     /protein_id="ACF06640.1"
                     /transl_table=11
                     /translation="MTLPSASGARVSRAPARPSPATVVFDRDGTLLDFYDMFHRFIVDL
                     HDAHGVAPPSREVILAYEYWQTITSGRLRIGDTVVRDRVDEVVHRYMPHGRLFPGTAEA
                     VRDLGAAGVRVVLVSSWVGTAATGELLERYGLRGAFGAVLTRDDLAADASLATDAECKT
                     VLARRALAEVGHQPGDALYLVGDTSADIASARMLGARAIGVRTGNGSRLAAVPDAGPDR
                     LVACAAEAAAVILREVRAG"
     gene            complement(11089..11676)
                     /gene="toyJ"
     CDS             complement(11089..11676)
                     /codon_start=1
                     /gene="toyJ"
                     /note="toyocamycin nitrile hydratase alpha subunit"
                     /product="ToyJ"
                     /protein_id="ACF06641.1"
                     /transl_table=11
                     /translation="MSTEHVSLPIAARVRRLEERVIAAGLVTDEQLDTILEHNLSRATP
                     FNGARLVARAWTSPDFRDLLLGEPAAALREMGLDGLLADDEHLRVVANTPTVHNVVVCT
                     LCSCYPVLLLGPSPSWYKSDAYRARVVREPRAVLAEFGTVLPAEVDVRVWDASAEARYM
                     VLPRRPAGTEGLDEEGLAARVTRAGLIGTAPV"
     gene            complement(11673..11984)
                     /gene="toyK"
     CDS             complement(11673..11984)
                     /codon_start=1
                     /gene="toyK"
                     /note="toyocamycin nitrile hydratase gamma subunit"
                     /product="ToyK"
                     /protein_id="ACF06642.1"
                     /transl_table=11
                     /translation="MGERRFSVGDPVRVRPADPPHHTRVPRYVRGHLGHVVTVQPPCPL
                     PDDVARRRDPFRVLPVYTVRFAARDLWGSGTHLVLIDLWECYLEPAAETARGLGGETS"
     gene            complement(11977..12252)
                     /gene="toyL"
     CDS             complement(11977..12252)
                     /codon_start=1
                     /gene="toyL"
                     /note="toyocamycin nitrile hydratase beta subunit"
                     /product="ToyL"
                     /protein_id="ACF06643.1"
                     /transl_table=11
                     /translation="MARLNDIGGTFGYGSIPMDGAEPPHPWRHDWEARVFAVLIGLAMA
                     GVWTASELRDAEERVPPNDYLAASYYERVLMGMELLLDEKGIPSRG"
     gene            complement(12269..12985)
                     /gene="toyM"
     CDS             complement(12269..12985)
                     /codon_start=1
                     /gene="toyM"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Asn_synthase"
                     /gene_kind="biosynthetic-additional"
                     /note="similar to ExsB"
                     /product="ToyM"
                     /protein_id="ACF06644.1"
                     /sec_met_domain="Asn_synthase (E-value: 3.1e-11, bitscore:
                     33.1, seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPESPVEADPFSAVVVLSGGMDSTTLLAHYAMLRYRLTAVTVDYG
                     QRHRREIDAARTIAGHYGAEHHVVDLSGIGVLLGGSALTDSEVDVPSGHYAEESMRATV
                     VPNRNAILANVAVGAAVARQAGIVALGMHAGDHFVYPDCRPAFVEALDGLVRVANEGFA
                     TPRVEAPFIHWSKTDIARYGSRLEAPLDKSWSCYRGGELHCGTCGTCYERREAFRDADV
                     PDPTEYLDAATEFAAP"
ORIGIN
        1 ccgcacactg tccgccgatg gggtcgatga accacccgtc cgccgtgccg ggctcactgt
       61 ggccggcgta cagcacaccg agcacgacga ccggcagcgt ggcgagggcc gcgagcagcc
      121 cgagccacgc gcgcaggggc gggggcagca ccgcggcggg cgccggccgt ccgggcacag
      181 acccgccgtg acagtccatc cggctgatgc gatgcacctc ccccacctga tcggcccccg
      241 actcaccgga tttgccgaag gtttggccga gggcaccggc atcggtctcc gcttgccacc
      301 ctagccacaa tcgggtgcgc gggacaccgt tgtcgtcagt tgacgcggca ggcccgtgga
      361 gttgctgacg taaatacagt tttcctcgcc cgttctcaac cttccggccc ccggtggcaa
      421 ctgttccctg aaagcatggc gcgaaatcca gggcggttga gcaggtacat gactgcattt
      481 gacgacgggg agcatcacgg acggtacgga aggcgcgaag ccgatgtccg tgaagccgtg
      541 tcggtgaccg ccttcaccgc ggcggagttc ggccgcgacg ggcacgacgg gccgttccgg
      601 aagcccgtcg gggacggtac ccacgcgacc ctcacggcac cggcgcgggc ccgtccgctg
      661 gtcgggcccg ccgtcgcgga ccgctccgtc ctccgcggca actgccccgg gcacggaccg
      721 gaagggtccg tttcgtcctc gtatccctca cctgtgtgaa ctacgctacg gcccgatgca
      781 cctgatacgt gcggcatcga cgtggtacgg cccggaaaag agccgcgccc gtttccggct
      841 tgcgctcgcc cccgggccgg ctgtccgccg tcccttttcc gcaccccgaa ctcaccgcga
      901 gacggacggt gtcccgccac cactgcatgc cgccggaccc atgacgagaa gagacagccg
      961 gcacgtcggc cggccggcac cgctcttccg gtcggcgagg gctcccccgc ggccccagcg
     1021 ctccacgccc ctgctcagct ccccgacgca aaggccccca cacatgaacc gtcgtccatt
     1081 gcatacgcaa ctccgtcctc cctccgcacc gaactcgacc gcacagcgtc cgaccgcccg
     1141 gggtgcacac ggagggagca aagacatatg agtgtggcaa cgccgacagc ggaagacacg
     1201 taccgcgcga aggactccgc ggtgctcgcc cctcgtccgg aaatgcgggt gttacgccag
     1261 gcactggccg acagcggcgc cggacggggc ggtcactggg tcgtgtacgg agaaaccggc
     1321 gcgggaaaga cctccgttct gcgcgccctt cgcgaagagg cccgcgtgct cggccacgcg
     1381 gccgtggaac tgaagtgcac gccgatggag gggtgcgtac cgttcgccac cgcgcgcaag
     1441 ctcgtcgcgc tgttcggcgc gtcactcccg gcgagaggtc ccgccgcgga agccctgcgc
     1501 cgggccggca gagcgctttc gggccgtaac ggctcggccg cgggccgggg ctcggggctg
     1561 ggcgtggtgc tcaccgggct ccaggaggcg gcccaggccc tcaccgcggc ggccccgctg
     1621 ctcatcacgg tcgacgacat cggctcggcc gacgccgcct cgctggactg cttctcctac
     1681 ctgctgaacc agtgcgaggg agtgcaccgc ctcctgatcg tcgcgacgct ggccgacggg
     1741 gaaccggtgc aggcgccggc cgcgctggag gggctgcggc tcggcgcccg gggacgtatc
     1801 gacctcggcg gtctcgacct gccgggcacg gccgaactgg tggcagccgt gaccggccgg
     1861 ccgtgtgacg agggcctcgc ggcggcctgc cgcacggcga cggcgggcaa cccgctgttc
     1921 ctgcgggccc tcgcgcggtg cctgccggcc cggcggcccg tccccgcgca cgagatcgcc
     1981 gggctggggc cgcgagccgt cgccgaccac gtactggccc ggctcgcgcg gatctcggag
     2041 ccggcgtcgg ccatggcccg tacggtcgcc gtactcggtg acgacaccga tccggcgatg
     2101 gtcgcggagc tggcgaacct ggactgctcg acgggcctgg aggcggcgga cgtcctggtg
     2161 cgcacgcggc tgttccacga cgggggacag atgtcgttcc gccatcccgt cgtggcggcg
     2221 gcgctccgcg aacagacctc catcgtcgtc aagaacaccc tgcacatgca ggccgccgag
     2281 catctgctca gccgtgacgc cccggccgag caggtggccg ggcacctgat ggcgaccacc
     2341 cttcccctca cgtcctcctg gatggtggac gcgctgcggt cggcggcgcg gtccgccctg
     2401 gtacgggagg aaacgggcac ggcagtgggg tacctgcaac gggccgtgca ggacgcgcgg
     2461 ggcgtggact ggcggcgggc ggtcgcgcag ctcgcggacg cacagctgct caccgacccc
     2521 tccgccgcca tcggccggct cgcggagctg gcccgccagg gcgtgaccgc cgaagagcac
     2581 ccgcagctcc tggcccggct cgcggacgcg tcgttcttct tcgccacccg cccccggata
     2641 cggcaccagc tggacgaggc ggtcgaggcg gtcgccgtct cgcagccgca ctacaccgcc
     2701 gacacacagc tgtaccgggc cctgcacgcg ctgtccgagc tgcccaccaa ggaagcgtgc
     2761 gaactcgggg accgctacct cgcgtccgag ccccgggaag gtgtgctgcg cagcgcggcc
     2821 ggggccctga gcgccgtgag cgcctatgcg gcgggccggg gcagaaaggc cgcggtggac
     2881 cgggtgaagg aggcgctacg cgaccccgac ctgtacgaac tccccaacac cgcgcccctg
     2941 tgcgccggca ccgcgctgct ggcgcacgag ggcgacctcg acctggcctc ggagtacgcc
     3001 cggcacggca tcgccgtcgc gcggcgggaa gggcggcagc tccaggtcgc cgcgctcctg
     3061 ccgaccctgg cgaagatcgc ccaggtacgc ggggacctgc gcgaggcgga gaacctgctg
     3121 cgcacctgcc tgaagacctt cgcgcggtac ggggtgtggc gggccaaccc ggccgccctc
     3181 tgggccggtt ccgtactggt cggcgtcctg gtcgacctgg gcgagccggc cgccgcccgc
     3241 acggtgctca gcgagagcgg ctgccacggc gacctgccgg cgatgtggtc gacgcaggcg
     3301 atgctggagc agcgggcccg ggtgcgcatg gcggccggag aactcaccga cgcgctggag
     3361 gacctgcgcg agtgcggccg gcgctcggcg gcgctgggcc tgaacagcgt cgagttcggg
     3421 ggctggcgcc ggctggccgc cgaggtgctg caccagttgg ggcggcccga cgaggcgagg
     3481 cggctggcgg aggaggagct ggcggccgta cggggccagg ccaccgaccg tacccaggga
     3541 gccgccctgc gcacggcagc acgggtcatc ggcggcgacg cgggcctggg gccggcgcgg
     3601 gaggcggtgc ggatcctggc gggcaccccc gacgtgctgg agctggcccg cgcccagctg
     3661 acgctgggca ccctgctcgg gggcaccggc gaccggaccg ccgcccaggg aaccctggcg
     3721 caggcgcacg cgctcgcgag ccgctgcggt gcggcgccgg tcgaggaaca ggcccgcgag
     3781 cagctgcacg ccctggggcg gcagcacggc ggccgggccg tgaccgggct gctgtccctg
     3841 accagacggg agcggcaggt actggccgat gccgtacgcg gccgcagcaa cggcgcgatc
     3901 gccgacgccc tcttcatcac ccggcgcacc gtggagattc atctgaccag tgcctaccgc
     3961 aaactcggca tccaagggcg caaggagttc gcgcagctct acaaccggca ggaattgtgg
     4021 gccctgttga cggaggaaac ggccggcagg tgacgacagg ccgtccggga cacccctctc
     4081 ccgtacgggc ggcactcaga tgagtggaca ggcggtcgga gcattgcgct ccggccgcct
     4141 tccggcgtcc tggagctgaa caggaggtac gggtttccac gtgccggagc ccgcggacaa
     4201 accttcggtg cggaccgaga ggcccgcgct tttcccagcg ccgaagatga acgcgaaccc
     4261 ggttcctggc cggagcggag gtcactcgcc ggccaggaac cggggaacac gcttgtttcc
     4321 ctttacccga agggcgagtc cgttgggatt ccgtatcagc aaagagttcc acttctccgc
     4381 gagccaccgt ctggaccacc tcgacgaagg ccatccctgt tcccgtatgc acgggcacaa
     4441 ctacgtcgtc gaactcgaac tgtccgccgg cgacgacgcc ctgaccaagg ccggcttcgt
     4501 ccgtgactac ggggagctcg cggccttcaa gcagtggctc gacgccgaac tcgaccaccg
     4561 gcacctcaac gacgtggtcg acggcctgaa cccgtccgcc gagaacctgg cgcggtggat
     4621 ccacgagcgg tggcgcaccg gcttcccgga gctgaccgcg gtccgggtct ccgagaccga
     4681 caagacgtgg gcggagtacc gggcatgacc acgcggcccg cggaggccgg gcgggagcgg
     4741 gaagggggtg gcgccgcggt gggaccgaag ctcgtggtga acgagatctt cggccccacc
     4801 tagctccgta cagggagaag gcccgtccgc cggccggcgg tgcgcgttcc tgcggctcgg
     4861 cggctgcaac ctctcgtgcc gctggtgcga caccccgtac acctgggact ggtcgggcat
     4921 cggcgactcc ggcgtcgcgt acgcgcccca ggacgagctg cacccgatgc cctggcggga
     4981 ggtgctggaa cggctgctgg cctacgaggt cgacctcatc gtcatctcgg gcggcgaacc
     5041 gctcaaccag cagcgccgcc tgctgcccct ggtgcgcgcg ctccgcgacc acggacgtga
     5101 cgtggagatc gagaccaacg gcacggtcgc gccggacgcc ggcctggtga ccggcggcgt
     5161 gcggttcaac gtctccccca agctgtccca ctccggcgac cccgagagcc ggcggatcgt
     5221 gcccgaggcg ctgcgggcgc tggccgcggt ccccggcacc gccttcaaat tcgtctgccg
     5281 cgacgaggag gacctggaag aggtcgcctc cgtcgtggac cgctgcggca cccggccggt
     5341 ctggatcatg cccgaaggcc agacgtccga agccacccac cgacatctcg cacggctcgg
     5401 cgatcacgtc gtagcacgcg gatggaacct caccacacgc cttcacgtgg ccgtgtgggg
     5461 cgacaggaga gccatatgac gacgagcgcc gaaacccgca cgctggagct gccgcgcgag
     5521 cacgcggagc cgggcaccga ccccctggag acgctggcac ggcagctcct caccgagatc
     5581 ggtgaggacc cggaccgctc cggcctgcgg gacacgccgg cccgcttcgc gcggtggtgg
     5641 agggagttca cccagtacga ccccggccag gtcgagaccg tcttcccgct gcacaccgtc
     5701 gggcagctgg tcacggtctc cgacatccat gtgtggtcgc tgtgcgagca ccacctgctc
     5761 ccgttctcgt gcacgctcac gatcgcctac cggccggcgt cccacgtgct cgggctctcg
     5821 aagttcgccc gtatcgccca ccgccacgcg caccggctgc agatccagga gcgcctcgtg
     5881 cacgacatcg cttccgagat cacggcggtg accgggtcgc ccgacgtggc cgtgatcggc
     5941 aagggcgagc acctgtgcat gagcatgcgg ggcatccgca cgccggcggt gatgacctcg
     6001 tcggcgttca ccgggatctt cgaggacttc gggccggcgc gcgacgaggt cgtgagcgcg
     6061 gccggcggac cccgctgacc gggcgggccc cgttccgccc cgccccgttc agctccgtcc
     6121 cgctccgttc cgtccgcccc caccggcaga cacagagaaa gaaggtgaaa ccgttgcgtg
     6181 aaatccccac cggcctcggg ctcgacgacg tcctgctcgt cccccagcgc acctcggtca
     6241 ccagccgttc gcacaccgac gtcagtaccg aactcgtccc gggcctgcgg ctgtccgtac
     6301 cgatcctgtc cgccaacacc ccgtggtgca ccggcgcccg gatggccgcc gccatggcgc
     6361 tggcgggcgg gctcggggtc atccaccgga tgcagacggc ggaggaccag gcggccgagg
     6421 tgaccgcggt gaagaaggaa catcccaagg aggagtccgc cgccccgggc gccacggtcg
     6481 acgagcgggg ccggctccgc gcggcggccg ccgtcggggt gacggacgat tacctcgacc
     6541 gggcggccct cctcgtcgag gccggggccg acgccctggt cgtcgatgtc gcgcacggcc
     6601 acgcggacta cgtcctcaag gccgtggagc agctgaagtc ccgctggccc ggcgtgcccc
     6661 tcgtgggcgg caacgtcgcg actcccgccg gcaccaggga cctgatcgac gccggggcgg
     6721 atgccgtgaa ggtcggcatc gggcccggcg gcatctgcac cacgcggctg gtggccggca
     6781 gcggcatgcc gcagttcacc gccgtactgg agtgcgcgga ggaggccgcg gggcgcggcg
     6841 tgccggtcat cgcggacggt ggcatcaagg agccgggcga cgtcgcgaag gtgctggcgg
     6901 ccggtgcccg gacggcgatg ctgggcagcg cgctcgccgg tgccgaggag agcgccgccc
     6961 ttctggtgga gcacgacggg cggccggcga aggtgagcac cgggttcgtg acgttcggca
     7021 tgcagctgac cctcaagcgg gcccgcggtg aggcggtgac caaggaggaa ctggcgcagt
     7081 acacgcccga aggcgtggag gcgacgtggc ccttcagcgg gccggccgcc ggcacggtga
     7141 accgtttcgt cggcgggctg cgctccggga tgagctactc gggcgcgcac accctcgccg
     7201 agttccgcga gcgggcctgc ttcgtccgcg tcacccccgc cggcctggcc gagaaccacc
     7261 cgcacgccct cggacgcgcc cagcaggtcc cgctcgacta cgcggcggag gcggtgtgac
     7321 gaccccgatc gacaccggcc ggtacggcag tccgcggatg cgcgcgctgt tcaccgaccg
     7381 ggcgcgctac gaggcgttcg tacgggtgga gggcgcgctg gccacggcgc aggggcggta
     7441 cggcgtcatt ccgcgggagg cggccgaagc catcgccgag caggcccgca cccaccgctg
     7501 ctccctggag cgcgtcgccg cgctcgaaga gacgagccgc cacgagctgt tcgccgccgt
     7561 acaggaattc gccgagtcct gcggcgccca cggcgcgtgg gtgcactacg gggcgaccag
     7621 cagcgacatc ctggacaccg cgctcgcgct ccagctcacc gaggccatcg cgctgctgga
     7681 ggaggacctg cgggacctcc tcaccgcgct gctggcacgg atcgacgcgg tcggtgacca
     7741 cgtgatgatc ggccgtaccc acggccagca ggcccagccg gtgacgctcg gattcaagct
     7801 ggccatcgtg ctggaccggt tcgtgcgctg cctggaacgg ctggcccagg ccaaagagcg
     7861 ggtcgtcatg ggcaagatcg cgggggcggt ggggtcgctc gccgggatcg gcgcgcacgg
     7921 ccccgaggtg cagcgggaga cgctgcggga gctgggcctc ccggaaccgg ccgtctgcgc
     7981 ccaggcagtg gcccgggacc ggatcgccga gttcttctgc tgggcggggc tgacggccgc
     8041 ctgcctggag gacctggcga ccgaggtacg caacctgcag cgtacggaga tcggcgaggt
     8101 cgccgaaccg ttcgccgagg gcggccagat cggcagctcc gccatgccgc acaagcgcaa
     8161 ccccgtacag agtgagcgca tctgcagcct ggcgcggctg gtgcgctcgc tcgtcgggcc
     8221 cgcgctggag aacgtgacga gctggcacga gcgggacctg gccaactccg ccaacgaacg
     8281 gttcaccctg cccgaggcgt gcgtcctgct ggacgagatg ctgaccaccg ccacgtacgt
     8341 gatcgacggc ctgcgggtct ttcccgaccg gatgcggcac aacctggagc actcgcacaa
     8401 cgagcacctc agcgaggcgg tcctgctgct cctcgtccag cgcggcatgg accgcctggc
     8461 ggcgtaccgc ctgctccagg aggccaccgg ccgggccacg ggcgaggacg gcgacatggt
     8521 cgacgccctg ctggcccatc cgcgcctcgc cggccggctc gacgcggaag aggtccgccg
     8581 gctggctgcc gcgggggccg acgtcgggca ctgccgggac ctgaccgccg cggccgtcaa
     8641 gcgcgccgag cacatcgtcc aaggaggatg acatgccctg caccatcatc gtgggcggcc
     8701 agtggggtga cgaggccaag ggcaagatgg ccagttacct ggcgctcaag gacggcgtgg
     8761 acctcgcggt gcgcgccggc ctcggcccgg gagccgggca caccatcgtg cacggcggac
     8821 ggacgcacaa actgcgccag gtcccgagcg ccgtggtcaa cgagcggacc cggctgctgc
     8881 tcggcgccgg tgtgctcgtc aatccggagg tactgctgcg ggagatcgac gagctggggg
     8941 tggccgaccg tatcggcgtc gacttccggg cgaccgtcat cgaccccggg cacgtcgagg
     9001 ccgagcagcg cgaacgcaat ctcaccgaca cggtccgcag caccggcagc ggacacggcc
     9061 ccgctctcgc ggcacgcgca ctgcgcaacg cccgccgggc gatggacgta cccgaactgg
     9121 ccccctacct ggcggacgtc gccgtggagg tcaacggcgc ggtggacgcg ggcgcgcagg
     9181 tccaggtgga ggggacgaac gggttcggcc tgtcggtcct gtacgggacc tacccgcaca
     9241 cggtcggcaa ggacagcacg gcgagcacgg cggccgcgga cgccgggctg ggtccgctgg
     9301 ccgtggacga cgtggtcctg gtcttccggg cctaccccac ccgcgtcggg gccggcgcgt
     9361 tcccgacgga gctgcccgag caggaggcgg agcgcatcgg ggtcgagtac ggcacggtga
     9421 ccggacggcg ccggcggatg ggccgcttcg acctggagca ggcccgggag gccgtacgga
     9481 tcaaccggcc ctcgcgcatc gccgtcagct tcctcgacta cctggcgccg gaggtccggg
     9541 ggcagcagga gggcccgctg ccgcccggcg cggcggccgg cctcgtcgaa gagatcgaac
     9601 gcgagctggg cgtacgggtc gagctcctcg gcaccggacc gggtacggac gaggtcatcg
     9661 acctgaggcc cgcccccgcc ggcgccggca ccacagcccc cctcctccgc acaaggagca
     9721 ccgcatgacc gccggaccgt ccctgtggaa gcggcagcga ctgctcacca tgagctggca
     9781 gacgctcggt acgctgctga ccggcctcgc cgaggagatc cgcgccgcgg gccccctccc
     9841 ggacaccgtc gtcggtatca cccgcggcgg gctcacccca gccgtcgccc tggcgcaccg
     9901 gctggacgta ccggagttcc ggatcatggg catcccgcgc aactcctcca acagccggta
     9961 cagcgagcgc ggcgcggcgg cactggagta cgtcgtcccg gacaccccgc tgacgggcaa
    10021 gcgcgtcctg gtcgtcgacg acatcatggg cgacggcggc acgatggaga ccgcggtcgg
    10081 caccctgcgt gccctggacg ccgcggagat ccgcaccgcg gtcctggtgc gcaaccagaa
    10141 ctgcgccggc cggccggacc accagggcgt cgaggtcgac gactggacgg tcttcccgtg
    10201 ggaggctccg ccgggcccgg cggagccgac cgtgccgctg gaggccgtgc ggtgacgctc
    10261 cccagcgcgt ccggggcgcg ggtctcccgt gccccggcgc ggccctcccc cgccacggtc
    10321 gtcttcgacc gggacggcac cctgctggac ttctacgaca tgttccaccg cttcatcgtc
    10381 gacctgcacg acgcccacgg ggtggcgccg ccctcccggg aggtgatcct cgcgtacgag
    10441 tactggcaga cgatcaccag cggccggctg cgcatcgggg acacggtcgt ccgcgaccgc
    10501 gtggacgagg tggtccaccg ctacatgccg cacggccgcc tcttccccgg caccgcggaa
    10561 gcggtacgtg acctgggcgc ggccggcgta cgcgtggtgc tggtgagcag ctgggtcggc
    10621 accgcggcga ccggggaact cctcgaacgg tacggactgc gcggcgcgtt cggcgccgtc
    10681 ctgacccgcg acgacctggc ggccgacgcg tccctggcca ccgacgcgga gtgcaagacg
    10741 gtcctcgccc gccgggccct ggccgaggtg ggacaccagc cgggcgacgc cctgtacctg
    10801 gtcggggaca cctcggcgga catcgcttcg gcccggatgc tcggggcccg ggccatcggc
    10861 gtgcgcaccg gaaacggcag ccggctggcc gcggtaccgg acgccggccc ggaccgtctc
    10921 gtggcctgcg ccgccgaggc cgcggccgtc atcctgcggg aggtgcgcgc ggggtgagcc
    10981 gcgtcccggc gcctgccgac gcgcgacggg ctgctctccc cgtccggccc tgagcagccg
    11041 gacggggaga gcagcccgtc gttttccttg tcctcccctt ccggctgctc acacaggagc
    11101 cgtgccgatg agtccggccc gcgtcacgcg tgcggccaga ccctcctcgt ccagcccctc
    11161 cgtccccgcg ggacgccgcg gcagcaccat gtaccgggcc tcggcgctgg cgtcccagac
    11221 ccggacgtcc acctccgccg gcagtacggt gccgaactcg gccagcacgg cacgcggctc
    11281 acgtaccacg cgtgcccggt acgcgtcgct cttgtaccag ctcggtgagg ggccgagcag
    11341 cagcaccggg tagcaggaac acagggtgca caccacgacg ttgtgcacgg tgggtgtgtt
    11401 ggcgacgacc cgcaggtgct cgtcgtcggc cagcagcccg tcgaggccca tctcgcgcag
    11461 cgccgcggcc ggctccccga gcagcaggtc ccggaagtcc gggctggtcc aggcacgggc
    11521 gaccaggcgg gccccgttga acggcgtggc ccgcgagagg ttgtgctcca gaatggtgtc
    11581 gagctgctcg tcggtgacga ggccggcggc gatgacgcgt tcttccagcc tgcggacccg
    11641 ggcggcgatg gggaggctga cgtgttcggt actcatgatg tttctcctcc aaggccccgg
    11701 gccgtctcgg cggccggttc caggtagcac tcccacaggt cgatcaggac gaggtgtgtc
    11761 ccgctgcccc acaggtcgcg cgctgcgaag cgcacggtgt agacgggaag gacccggaac
    11821 ggatcccggc gccgggccac gtcgtcggga agcgggcagg gcggttgtac ggtgacgacg
    11881 tgcccgaggt gaccccgtac gtaccggggc acccgggtgt ggtggggcgg atccgccggg
    11941 cgcacccgga ccggatcccc gacggagaac cgccgttcac ccacgggagg gaatcccctt
    12001 ctcgtccagg aggagttcca tgcccatgag cacccgctcg taataactgg ccgcgaggta
    12061 atcgttgggc gggacgcgct cctcggcgtc gcggagttcc gaggcggtcc agacaccggc
    12121 catcgccagg ccgatcagca cggcgaacac ccgtgcttcc cagtcgtgcc gccacgggtg
    12181 cggcggttcc gcaccgtcca tcggaatgct gccgtaaccg aaagtgccgc cgatgtcgtt
    12241 cagccgtgcc atcagtttcc cggtcctttc acggcgctgc gaattcggtg gcggcgtcga
    12301 ggtattccgt cgggtccggc acgtccgcgt cacggaatgc ctcacggcgt tcgtaacacg
    12361 taccgcaggt accgcagtgg agttctccgc cgcggtagca cgaccagctc ttgtccagcg
    12421 gggcttccag ccggctcccg taccgcgcga tgtccgtctt gctccagtgg atgaatggcg
    12481 cttccacccg cggcgtggcg aatccctcgt tcgcgacccg gacgagtccg tccagcgcct
    12541 cgacgaacgc cggccggcag tcgggataga cgaaatggtc accggcgtgc attccgagag
    12601 ccacgatgcc ggcctgccgc gcgaccgcgg cgcccaccgc cacattggcc aggatggcat
    12661 tgcggttggg gacgacggtg gcccgcatcg actcctcggc gtagtgccct gaggggacgt
    12721 cgacctcact gtcggtcagg gcggagccgc ccagcaggac cccgataccg ctcaggtcca
    12781 ccacatggtg ttccgcgccg tagtgcccgg cgatcgtccg ggccgcgtcg atctcccggc
    12841 ggtggcgctg accgtagtcg accgtcaccg cggtgagccg gtagcgcagc atggcgtaat
    12901 gggcgagcag cgtcgtggag tccatgccgc cggacagcac gacgacggcg ctgaacgggt
    12961 cggcctcgac cggcgattcg ggcaagacgc acctcctggg tgtcggggga acgacgcgcc
    13021 gcgcagccgg ccgccgtctt cgatcaccac ccagcgtccc ggcagccacc ccgccgggcc
    13081 tcacggtgcc gtccgagatt tcacggaccg gaccgagaga aagcgccggg gccccgagca
    13141 tcacctccta cgggggatgc cgggaccgcc cggtctgtcg ctcaatggcg gtccactccc
    13201 agggagacag gagggcccgg catgacgacc gagaccggcc ccgtacagct gctgacgatc
    13261 gcgttcggcc cggacgcgaa gttcgagggg cggatcatcg aggaactcga cggcctcacg
    13321 gccgcgggcc agatcctcgt actggacctg ctggtcgtac agaagcagcc cggcggtgac
    13381 ctcgtctccg tcggctacca ggcggaaggc atgggcgaca cggtcagcgc cctgctcgga
    13441 ctgggtccgg gcggcctgcg gggagcggag gcggcgttcc cgtccctggc accgggccgc
    13501 gccttcggcc tcggcgcgga cgacgtccgg cagctcgccg actccctgga cccgggcacc
    13561 accgcggctt tcctgctgct cgaacaccgc tgggcgcgga agctgcggca cctggtccgt
    13621 gagcagggag gcgtgcccgt ggccgagggg ttcctcaccg aagaagcggt cgcccccatc
    13681 gccgcggagc tgctcgccgc cgccacacgg ctggacgagg ggccgacggg agaaagggga
    13741 gggtcatgag cggttccgtc accctcgcgt acgactatcc gctgctcggc gtcttctgga
    13801 ccacgttctg gctcttcatg tgggtgctgt ggttcttcct gctcttccgc gtcatcggcg
    13861 acatcttccg tgacgacggg atgaacggct gggccaagac cggctggatc ctcttcgtcc
    13921 tggtgctgcc cttcctcggc gtcctcgtct acgtcctcgc ccggggccgc ggcatgggcc
    13981 ggcgcgaggt gaaccgggcg caggcccagc aggaggcgtt cgacgcgtac gtacgccgga
    14041 ccgccacggc gggcacgccc cgcgccaccc cggccgacga gctgacgaag ctctcccagc
    14101 tcagggagcg cggcgacatc tcggaggagg agttccagcg ggcgaagggc gtggtcctgc
    14161 actgacttcg gtggtggcac cggtggtcgt gctgtcctgg cgggttcccg gacggaggta
    14221 gccatggcgg gagacatcgc agggtcaccg gccggcccct ctcctcagga gggcggcggg
    14281 gcggcggccg acggctccgg tccgtcgtcg ccgaagctcc gccgtcgccg ggggctgtgg
    14341 tccgtcaccc tgcccgggtg ctggggagcg ctgctgttcg cctgcctctc cttcaccccg
    14401 tcgctgctcc cgcgcggcgg catcctgcag ggcctgatct gcggtatcga cgcggccctc
    14461 gggtacgcgg ccggggtgct cgccgcgcgg gtgtggcgcg cgttcgccga ccgggagccg
    14521 cggcctccgc ggcgcaggtc ctggaccgtc ttcttcctcg gcgcggtcct cctcttcggc
    14581 ctctccttcg gcttcgggca gtactggcag taccgga
//
