LOCUS       BGC0000880             11994 bp    DNA     linear   BCT 15-NOV-2010
DEFINITION  Streptomyces chartreusis strain NRRL3882 tunicamycin biosynthesis
            gene cluster, complete sequence.
ACCESSION   BGC0000880
VERSION     BGC0000880
KEYWORDS    .
SOURCE      Streptomyces chartreusis NRRL 3882
  ORGANISM  Streptomyces chartreusis NRRL 3882
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 14:22:14
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 11994
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     gene            1..966
                     /gene="tunA"
     CDS             1..966
                     /codon_start=1
                     /gene="tunA"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     nucleoside: TunD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010:NAD-dependent epimerase/dehydratase (Score:
                     208.1; E-value: 3.4e-63)"
                     /gene_kind="biosynthetic"
                     /note="putative UDP-GlcNAc epimerase/dehydratase"
                     /product="TunA"
                     /protein_id="ADP94221.1"
                     /sec_met_domain="TunD (E-value: 2.7e-149, bitscore: 486.3,
                     seeds: 3, tool: rule-based-clusters)"
                     /sec_met_domain="RmlD_sub_bind (E-value: 8.8e-15, bitscore:
                     44.4, seeds: 37, tool: rule-based-clusters)"
                     /sec_met_domain="Polysacc_synt_2 (E-value: 2.2e-07,
                     bitscore: 20.1, seeds: 77, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQRNTLKHRILITGGAGFIGGHLARALVASGEEVTVLDDLRVPPM
                     IPPEGTGKFLEKPVLELEERDLSDVRLVYHLASHKSVPRSFKQPLDYLDNVDSGRHLLA
                     LCTSVGVPKVVVGSTCEVYGQADTLPTPEDSPLSPRSPYAASKVGLEMVAGAHQRASVA
                     PEVGIVRFFNVYGPGERPDALVPRLCANLLTRNELPVEGDGEQRRDFTYITDVVDKLVA
                     LANRPLPSVVNFGSGQSLSVNDVIRILQATSPAAEVARKQPRPNEITEFRADTALQTRQ
                     IGERSGGIGIEEGIRLTLEWWQSRDLDDIRQRIFQEEGAD"
     misc_feature    1..11994
                     /note="tunicamycin biosynthesis gene cluster"
     subregion       1..11994
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000880"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..11994
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="nucleoside"
                     /region_number="1"
                     /rules="(LipM or LipV or LipU or ToyB or TunD or pur6 or
                     pur10 or nikJ or nikO or truD)"
                     /subregion_numbers="1"
                     /tool="antismash"
     protocluster    1..10966
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[0:966]"
                     /cutoff="20000"
                     /detection_rule="(LipM or LipV or LipU or ToyB or TunD or
                     pur6 or pur10 or nikJ or nikO or truD)"
                     /neighbourhood="10000"
                     /product="nucleoside"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1..966
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(LipM or LipV or LipU or ToyB or TunD or
                     pur6 or pur10 or nikJ or nikO or truD)"
                     /neighbourhood="10000"
                     /product="nucleoside"
                     /protocluster_number="1"
     cand_cluster    1..10966
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(LipM or LipV or LipU or ToyB or TunD or
                     pur6 or pur10 or nikJ or nikO or truD)"
                     /kind="single"
                     /product="nucleoside"
                     /protoclusters="1"
                     /tool="antismash"
     misc_feature    163..165
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    178..180
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    184..186
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            966..1982
                     /gene="tunB"
     CDS             966..1982
                     /codon_start=1
                     /gene="tunB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF04055"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_12"
                     /gene_kind="biosynthetic-additional"
                     /note="putative uridine Fe-S oxidoreductase"
                     /product="TunB"
                     /protein_id="ADP94222.1"
                     /sec_met_domain="PF04055 (E-value: 8.7e-19, bitscore: 58.4,
                     seeds: 518, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_12 (E-value: 6.3e-08, bitscore: 22.9,
                     seeds: 84, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTGYTAPVRASLDVTYACDLRCIHCRTNTGEIPVHIRRKMLSIEQ
                     LQDIMLQLDRMGTFEITLTGGEPTIRKGFWQLLDVVPKLRHSTVTLITNAAAHTREQLD
                     RIVDSGISSIRVSMDGTRETFAAVRLMDVFDTVVENSIYLRDRVRSFKVLTTVMKTNQH
                     NVFDLAHYLRAHGFRRQDLILVRAHGRGGRNRLLLTEEETMAVHRKVTEFKRDVPATDF
                     DLNLNAPYLVPGEKLRPFQDVVMFPYLVRDSSIAISATGDVTMSRLYSAAPLGNTKDAP
                     VQEIWTQGQQQLVQEQEEFTEERLREIFWDFATSDGPDGPPLTSLLDRQIFEGAEVR"
     gene            1986..2942
                     /gene="tunC"
     CDS             1986..2942
                     /codon_start=1
                     /gene="tunC"
                     /note="putative N-acyltransferase"
                     /product="TunC"
                     /protein_id="ADP94223.1"
                     /transl_table=11
                     /translation="MSREALIRLYDLTPSQPLLDALSPATASRDIAPVVPRFKGAAGPR
                     AQSFVELHREGTLLGRCGINVKGPGTVGACEVAAVVAPAERAGMHWLLVHVALERLQWL
                     GYAYAMTEVSEYADHFPSVLRQAAWWIPDSSERKSAAARDDKSLEWADLFIDFRTWTPS
                     STPTSLTVNGRDLWVRRPEASEELLIVDWLRETFGGGWASEIHRSFSRDPISSVIVVDR
                     NKELPPKDRLLGFLAYDTARLGMLSAIALVPETRGRDLSLATALIEECLREARASGMTY
                     AVLGGVGNARLAALRTFSALWTIPGSCPGIFGRGVRN"
     gene            2942..4366
                     /gene="tunD"
     CDS             2942..4366
                     /codon_start=1
                     /gene="tunD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1045:glycosyl transferase group 1 (Score: 146.6;
                     E-value: 1.9e-44)"
                     /gene_kind="biosynthetic-additional"
                     /note="putative N-acetylglucosamine transferase"
                     /product="TunD"
                     /protein_id="ADP94224.1"
                     /sec_met_domain="Glycos_transf_1 (E-value: 9.4e-36,
                     bitscore: 112.9, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEIIFTVSDQVWGGKHRYMHDMALGLAQAGHTVTVLAEEGGAMLQ
                     QCRAAGVTTVSVPAFASDDAAEAVRKALRHRRPHIVCVSGRADAAAVHHAQSQGVTDAA
                     VCLFRHSAFPLGTTDEVRDLFTGVNLVFTTSLEQRQRQFEPLINXXAGVLKDEQVEILT
                     SGVGEPLLAALDAADRGAARKELRAESDQFVFLVLARLAWEKGIDQVIDAFADLELPPD
                     AAPPLLVVAGEGPLEAELRGQTIERGVAERVQFLGHQDHVAPVIKASDAVVLTSTVPET
                     GPLALKEAMAAGRPVIASVQGGIPEFVEDERHGLLVIDDEDLRQAMQRLLSDREAAETM
                     GAAGSESVRGGHRAVRRVEYLAHRLDLLALEQLAPDTVLHEVVWDDVRLREETQGGFVF
                     VPRTSHIMELDSATYAVVRTAVEAGDPLQLIKLPEETLGVIAHRLYAMGALVRQDGQAT
                     PAARTADGERPVTEPA"
     gene            4363..5067
                     /gene="tunE"
     CDS             4363..5067
                     /codon_start=1
                     /gene="tunE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PIG-L"
                     /gene_kind="biosynthetic-additional"
                     /note="putative N-deacetylase"
                     /product="TunE"
                     /protein_id="ADP94225.1"
                     /sec_met_domain="PIG-L (E-value: 9.4e-24, bitscore: 74.5,
                     seeds: 137, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKVLVIAAHPDDEVLGAGATVSALSQQGAVVQIHILAEGISLRHS
                     GVKIEEARERCQVAAKDLGAAEVSFGGLAADGRLLADLPQRQVVDAVSHALREAEPEVV
                     FTHHPGDIHADHRSVAHAVGYGTRILGSGSVRQVLHFEVLSSTEQQTGLVAPFTPNLFY
                     DVTGHVEAKCRALAAYPYELYDPPHPRSLAAVRTLASYRGTQVGVEAAEAFMIGRELRG
                     PMAAVPSGVDVR"
     gene            5064..6047
                     /gene="tunF"
     CDS             5064..6047
                     /NRPS_PKS="Domain: NAD_binding_4 (66-163). E-value:
                     7.1e-11. Score: 33.5. Matches aSDomain:
                     nrpspksdomains_tunF_NAD_binding_4.1"
                     /codon_start=1
                     /gene="tunF"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010:NAD-dependent epimerase/dehydratase (Score:
                     273.4; E-value: 4.6e-83)"
                     /gene_kind="biosynthetic-additional"
                     /note="putative UDP-GlcNAc-4-epimerase"
                     /product="TunF"
                     /protein_id="ADP94226.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 5.1e-21, bitscore:
                     64.8, seeds: 37, tool: rule-based-clusters)"
                     /sec_met_domain="Polysacc_synt_2 (E-value: 1.1e-15,
                     bitscore: 47.3, seeds: 77, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRVLVTGGAGYVGSFTVRRLLAAGHEVVVVDNLSTGRREAVRGCR
                     LHVVDILDIASMGTVFEEFHPEAVIHFAALKSSEESLRDINTYFSVNLTGTQNVLALCA
                     RTGVERFVFSSSCAVYGTPQICPVDETAPVRPESPYGETKYLCERVIASYAQATGMRYA
                     NLRYFNAAGAADDASLGEYAGPASTQLLPVAIRSTLGLAPTLRIFGNDYPTTDGTALRD
                     YIHVEDLAQAHVRVLEGLQKGSQCGPVNLGRGQPVSVRELIDVLHDVSGKETPVAMAPR
                     RPGDPGLSWADPSLALSRFGWRAERDLDHIVRTAWNWHTSTPASLE"
     aSDomain        5262..5552
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_tunF_NAD_binding_4.1"
                     /evalue="7.10E-11"
                     /label="tunF_NAD_binding_4.1"
                     /locus_tag="tunF"
                     /protein_end="163"
                     /protein_start="66"
                     /score="33.5"
                     /tool="antismash"
                     /translation="EAVIHFAALKSSEESLRDINTYFSVNLTGTQNVLALCARTGVERF
                     VFSSSCAVYGTPQICPVDETAPVRPESPYGETKYLCERVIASYAQATGMRYA"
     gene            6049..6660
                     /gene="tunG"
     CDS             6049..6660
                     /codon_start=1
                     /gene="tunG"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1101:phosphoglycerate mutase (Score: 73; E-value:
                     4.2e-22)"
                     /gene_kind="biosynthetic-additional"
                     /note="putative UMP phosphatase"
                     /product="TunG"
                     /protein_id="ADP94227.1"
                     /transl_table=11
                     /translation="MHLYLLRHAEDRSGKFDPHQDRLTERGRRQAQEAAHWLAERRPTE
                     LRTSVLPRAQQTADIVAAHTGLAAREDPRLNEIVWSPVAGEFLPNPREDRLHPPPPGAE
                     SWNVFMTRVATCMSELCHQASEDQRIVLVTHSGFFDAVNELLCGGTGRVELLVAHAGIT
                     HWQYRPGDSGGAWLLHQHNVTFAPQTLMDHGSLSTAELSA"
     gene            6657..8204
                     /gene="tunH"
     CDS             6657..8204
                     /codon_start=1
                     /gene="tunH"
                     /note="putative UDP-tunicaminyluracil pyrophosphatase"
                     /product="TunH"
                     /protein_id="ADP94228.1"
                     /transl_table=11
                     /translation="MSGRRRRMVVICVDGGLPGIIREHDFFNLSRALPTLPGTAVHELR
                     SIYPSSTAPSHASFLTGTYPSGHGIVGNRFWERESVEEIRRRSDDPLSSFHPYEESSLT
                     APSLLDWFARQGASAAAVHFPQTFSRNAQLAIPSCYCLYAPARNLVVPLGPTVDGAAEG
                     VVQLSYLGHEVALYLRVDQQTNVITVGSHRETAVVADSLRPTRLDIPVSSGSVSVAVSC
                     RRLDEGQIEVRLGTAVITLGFGGLDMPDRAGDGPASLYVEYTANPGHTFHESPRAEWVE
                     QTALDVLKQHDPDVLFVRFNQADHAQEFLYWHAVRGSSGERSRAWQQILDVYARIDACV
                     MRLAQAAGEHAEFLFFSDHGIDYVETHLRPNHVLADLGLADRMVFQGDSNCAYLYADAP
                     VPPAEKKRLDQALHALDPSIGVLTPAEQDRLKLPVGSPRLGRLTVTCGPHTEFQYSDAT
                     PRESVASASHGYLPSDSAMSGFFRCFGQAAARPAPPKDITGAATAVRSIWRQAAREM"
     gene            8207..9121
                     /gene="tunI"
     CDS             8207..9121
                     /codon_start=1
                     /gene="tunI"
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 198.3; E-value:
                     2.4e-60)"
                     /gene_kind="transport"
                     /note="putative ABC transporter ATP-binding subunit"
                     /product="TunI"
                     /protein_id="ADP94229.1"
                     /transl_table=11
                     /translation="MPENPPYAYEVEGLHKQFDGVPRPANGEISFHIKQGEFFGLLGSN
                     GAGKTTLIKQMIGLVRPDGGTVRLLGRDVFAHPRFTAATVGYMPQTAFALNSLTVKEAV
                     YFSAHLRGVAAKAARRERDALLELLGLTHLAGKVARQLSGGERRLLQIAVTLIGSPPVL
                     ILDEPTNELDPSRRRQIWQLLRELNQRDGHTIILITHNAIEAEQVIERVGILKEGRLVA
                     LGAPAELKSSLRSQVRLSITVGDTQMHGLPDYVRVQSDHRGKVVALVDSADLPHLTKDV
                     DLTQFPEFTMHTATLEDVYVSYA"
     gene            9105..9893
                     /gene="tunJ"
     CDS             9105..9893
                     /codon_start=1
                     /gene="tunJ"
                     /note="putative ABC transporter permease subunit"
                     /product="TunJ"
                     /protein_id="ADP94230.1"
                     /transl_table=11
                     /translation="MSATRDKKLGLSFLGQVWDLFRIQLTNWRWSWPQMVLTGVLAPLV
                     SIAALGLFARNSGHAATEYVLTGSVTMAILFETQNKIASNFAFMRGNGAFEYYAALPVR
                     REALIFATLAAFSILSLPAIAVTLLLGSALLDVPLTFSPLAPLCLLLALLPSAGLGAFI
                     GSRSGTIEQASSLSLATTLLMMAAGPVAVPPELLPDALIWIGNLNPAVYAADSLRHSLT
                     APDASRALSDLAILAIFAGAVWGLTSTRMHWRARYGRRAS"
     gene            9933..10178
                     /gene="tunK"
     CDS             9933..10178
                     /NRPS_PKS="Domain: PP-binding (12-74). E-value: 1.1e-12.
                     Score: 40.2. Matches aSDomain:
                     nrpspksdomains_tunK_PP-binding.1"
                     /codon_start=1
                     /gene="tunK"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_kind="biosynthetic-additional"
                     /note="putative acyl carrier protein"
                     /product="TunK"
                     /protein_id="ADP94231.1"
                     /sec_met_domain="PP-binding (E-value: 2.9e-13, bitscore:
                     40.2, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTEQQFLAVLAPLIEEITGNAFSQEQLDVTFGDLDIDSLNQIEII
                     SRIEDEFDCHIEDSTLRTIRTPRGLLDHISKSTSPA"
     aSDomain        9969..10154
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_tunK_PP-binding.1"
                     /evalue="1.10E-12"
                     /label="tunK_PP-binding.1"
                     /locus_tag="tunK"
                     /protein_end="74"
                     /protein_start="12"
                     /score="40.2"
                     /tool="antismash"
                     /translation="LIEEITGNAFSQEQLDVTFGDLDIDSLNQIEIISRIEDEFDCHIE
                     DSTLRTIRTPRGLLDHI"
     gene            10175..10864
                     /gene="tunL"
     CDS             10175..10864
                     /codon_start=1
                     /gene="tunL"
                     /note="putative phospholipid phosphatase"
                     /product="TunL"
                     /protein_id="ADP94232.1"
                     /transl_table=11
                     /translation="MIRNHSGDRPRSLAWFAWWAAVHAGLLLVLVTFRWDERLSETLCA
                     WTSSSASSARLLETLTSWGNPWPVTIIAAGSACFALIRRNVTAAAVLLVIPYAATVSCN
                     LLKGWAERQRPALDCASIQADGFSFPSGHAVGVTTGFMLAALYFSSQMPRAVSALMLFL
                     AGLSAELVAWSRVLLGAHFSVDVLAGQLLGAGWLAVAALVLHRQSVRRLQREARQAGSN
                     SLDEPVS"
     gene            10872..11522
                     /gene="tunM"
     CDS             10872..11522
                     /NRPS_PKS="Domain: oMT (10-120). E-value: 1.1e-08. Score:
                     25.3. Matches aSDomain: nrpspksdomains_tunM_oMT.1"
                     /codon_start=1
                     /gene="tunM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1248:methyltransferase (Score: 57.9; E-value:
                     1.8e-17)"
                     /gene_kind="biosynthetic-additional"
                     /note="putative methyltransferase"
                     /product="TunM"
                     /protein_id="ADP94233.1"
                     /transl_table=11
                     /translation="MPFNHNDHYHDLLLRELPESCRRALEVGCGTGRFAQKLALRGIEV
                     DAIDVDNDVVTAAREMNSTLDLPRQIRFERADITHLTLTPDTYDYIACLASIHHVPFET
                     VQKLRASLTTNGVLVILGCYREATIWDHAISLLAVPVNAIWRAAVFFRESWSGSRPDRG
                     TQSPSAPVAPPSMSLAEIREDSAEYLPGRRIRRLLFWRYLLVFHNMKAGLGSD"
     aSDomain        10902..11231
                     /aSDomain="MT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_tunM_oMT.1"
                     /domain_subtypes="oMT"
                     /evalue="1.10E-08"
                     /label="tunM_oMT.1"
                     /locus_tag="tunM"
                     /protein_end="120"
                     /protein_start="10"
                     /score="25.3"
                     /tool="antismash"
                     /translation="DLLLRELPESCRRALEVGCGTGRFAQKLALRGIEVDAIDVDNDVV
                     TAAREMNSTLDLPRQIRFERADITHLTLTPDTYDYIACLASIHHVPFETVQKLRASLTT
                     NGVLVI"
     misc_feature    10908..10910
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            11536..11994
                     /gene="tunN"
     CDS             11536..11994
                     /codon_start=1
                     /gene="tunN"
                     /note="putative UTP pyrophosphatase"
                     /product="TunN"
                     /protein_id="ADP94234.1"
                     /transl_table=11
                     /translation="MISQPPRRYALGATCVTFNDVGRVLIARRRSPERWELPGGLVDPG
                     EAFHDAATRETYEETGVHVKVHGLVGVYQHPSRGILAGIFIATALSGEPRPTAESIAAE
                     WADVEDALTRLHPLYRPRLEDALTARASVTLRVHEGTEVLSLFEATHV"
ORIGIN
        1 gtgcaaagaa acactttgaa acaccggatt ctcatcaccg gaggcgccgg attcatcggt
       61 ggccaccttg cccgcgcact ggtggcgtcc ggggaagaag taacagtcct ggacgacctg
      121 cgtgtgccgc cgatgatccc gccggaaggc accggaaagt tcttagaaaa acccgtctta
      181 gagttagagg aaagggatct ctctgacgta cgcctcgtct accacctggc gtcgcacaag
      241 agtgttcctc gctccttcaa acagccgctc gactatctcg acaacgtgga ctcgggccgc
      301 cacctgctgg ctctctgcac gagcgttggc gtgccgaagg tggtcgtcgg gagtacgtgc
      361 gaggtctacg gccaggccga cacactaccg acacccgaag atagccctct atccccgcgc
      421 tcgccctacg ccgcgagcaa ggtcgggctg gagatggtgg ccggggccca ccagcgcgcc
      481 tcagtcgctc ccgaggtggg catcgttcgc ttcttcaacg tctacggccc cggcgagcgg
      541 cccgacgccc tcgtaccccg gctctgcgcc aacctcctga ccaggaacga actgcctgtc
      601 gagggcgacg gtgagcagcg tcgcgacttc acctacatca cggatgtcgt ggacaaactc
      661 gtcgcgctgg cgaaccgacc actgccctca gttgtcaact tcggttccgg ccagtccctc
      721 tccgtgaacg acgtcatccg gatcctgcag gcgacctctc cggcggccga ggtcgcccgg
      781 aagcagcctc ggccgaacga aatcacggag ttccgggcgg acaccgcctt gcagacgcgg
      841 cagatcggag agaggtccgg cgggatcgga atcgaggaag gcattcgcct cactctcgaa
      901 tggtggcagt cgcgggacct tgacgacata cgccagcgta tcttccaaga ggagggggcc
      961 gactgatgac cggctacacc gcacctgtac gcgccagtct ggatgtcact tacgcatgcg
     1021 acctgcgctg catccactgc cgcacgaaca ccggggagat ccccgtccac atccgccgca
     1081 agatgctgtc catcgagcag ttgcaggaca tcatgctcca actggaccgg atggggacat
     1141 tcgagatcac cctcaccggt ggggagccga cgatccgcaa gggcttctgg cagctcctgg
     1201 acgtcgttcc gaagctgcgc cattcgaccg tcactctcat cacgaacgcg gcagcgcaca
     1261 cgcgggaaca gctcgaccgc atcgtggaca gcggcatttc gtccatccgg gtcagcatgg
     1321 acggcacgcg ggagacgttc gccgccgtac ggctcatgga cgtgttcgac accgtcgtgg
     1381 agaactcgat ctacctccgc gaccgtgtgc gcagtttcaa ggtgctgacc acggtcatga
     1441 agaccaacca gcacaacgtg ttcgacctcg cgcactacct gcgagcccac ggcttccggc
     1501 gccaggacct catcctggtc cgggcgcacg gccgcggtgg ccgcaaccgt ctgctcctga
     1561 cagaggagga gaccatggcg gtccaccgca aggtgaccga gttcaagcgg gacgtgccgg
     1621 ccacggactt cgacctgaac ctcaacgcgc cctacctggt cccgggcgag aagctgcgcc
     1681 ccttccagga cgtcgtcatg ttcccctacc tggtgcgcga cagcagcatc gcgatctccg
     1741 ccacaggcga cgtgaccatg agccgcctct acagtgccgc gcccttgggg aacaccaagg
     1801 atgctcccgt gcaagagatc tggacgcagg ggcagcagca gctcgtccaa gagcaggagg
     1861 agttcaccga ggagcgtctg cgcgagatct tctgggactt cgcgaccagt gacggaccgg
     1921 acggtccccc gctcacctcg ctgctggacc ggcagatctt cgaaggcgcg gaggtgaggt
     1981 aggccttgtc cagggaggcc ctcatacgcc tgtacgacct cacgccgtcc cagcctctgc
     2041 tcgacgctct gagccctgcc accgcatcgc gggatatcgc tcctgtcgtt ccccggttca
     2101 aaggggccgc tgggccacga gcccagtcct tcgtcgaact gcaccgggaa gggacgcttc
     2161 tgggccggtg tggcatcaac gtcaagggcc cgggcacagt cggcgcctgc gaggtggcgg
     2221 ccgtggtcgc cccggcggag cgggcgggca tgcactggct tctcgtccat gtcgcactcg
     2281 agcggcttca gtggctgggg tatgcctacg cgatgacgga agtcagcgaa tacgcggacc
     2341 acttcccgtc ggtgctgcgg caggcggcgt ggtggattcc cgattccagc gaacgcaaga
     2401 gcgcggcggc gcgggacgac aagagcctgg aatgggcgga tctcttcatc gacttccgga
     2461 cctggacgcc aagttctact cccacgtctc ttacggtcaa cggccgagac ctgtgggtgc
     2521 gtcgtcccga agcgagtgag gaactgctga ttgtcgactg gctcagagag accttcggcg
     2581 gaggctgggc gagcgagatc caccggtcgt tctcacggga tccgatctcg tctgtgatcg
     2641 tggtcgaccg gaacaaggaa ctgccgccca aggaccgctt gctcggcttc cttgcctacg
     2701 acactgcgcg gctcgggatg ctgtctgcga ttgcgctcgt gcccgagaca cgcggtcgcg
     2761 atctgtcctt ggcgacagcg ctcatcgagg agtgcctgcg cgaagcgcga gccagcggca
     2821 tgacctatgc cgtgctggga ggcgtaggca acgcccgctt ggcggctctt cgcacattca
     2881 gcgctctatg gaccatccct ggctcgtgcc cgggaatctt cgggaggggt gttcggaact
     2941 aatggagatc atcttcactg tctccgatca ggtatggggc ggcaaacacc gctacatgca
     3001 cgacatggcg ctcgggctgg cgcaggcagg ccatacggtc accgtcctcg cggaggaggg
     3061 tggagccatg cttcagcagt gccgtgccgc cggggtaacg accgtgagtg tgccggcgtt
     3121 cgccagcgac gacgcggccg aggcggttcg caaggcgctg cggcatcgtc gtcctcatat
     3181 cgtgtgtgtc tccggccgcg cggacgccgc cgcagtccac cacgcccagt cgcagggcgt
     3241 gactgatgcc gccgtgtgcc tgttccggca ctcggcgttc cctctgggaa ccaccgacga
     3301 ggtgcgcgac ctattcaccg gcgtgaacct ggtcttcacc acgtcactgg aacagcggca
     3361 acgccagttc gagccgctga tcaacnnnnn ngccggtgtc ttgaaggacg aacaggtcga
     3421 gatactcacc agtggcgtcg gggagcctct gctcgcggct ctggacgccg ccgaccgcgg
     3481 cgccgctcga aaggaactcc gagccgaatc ggaccagttc gtcttcctcg tgctggcccg
     3541 cctggcctgg gagaagggca tcgaccaggt gatcgacgcc ttcgctgacc tggagctgcc
     3601 gccggacgcc gcgcccccgc tgctggtggt cgcgggtgag gggccgctgg aggcagaact
     3661 acgaggccag accatcgaac ggggtgtagc cgaacgggtc caattcctcg gccaccagga
     3721 ccacgtcgcc ccggtgatca aggccagtga cgccgtggtc ctcaccagca cggttcccga
     3781 gaccggcccg ctcgcgctca aggaggccat ggccgccggc cgccccgtca tcgcctctgt
     3841 ccagggcggc atcccggagt tcgtcgagga cgagcggcac ggtctgctgg tcatcgacga
     3901 cgaggacctg cggcaggcga tgcagcggct cctgagcgac cgcgaagccg ccgagaccat
     3961 gggagccgcc ggatccgagt cggttcgcgg cggacatcgt gcagtacgac gcgtggagta
     4021 cctcgcccac cgtctcgatc tcctggccct ggaacagctc gccccggaca cggtccttca
     4081 cgaagtggtg tgggacgacg tacggctgcg tgaagagaca caaggcggct tcgtcttcgt
     4141 gccgcgtaca tcgcacatca tggaactgga cagcgctacg tacgctgtcg tgcgcaccgc
     4201 ggtcgaagcc ggtgacccac tccagttgat caagctcccc gaagagacgc tcggtgtgat
     4261 tgcacaccgg ctctacgcca tgggcgcgct ggtccggcag gatggtcagg cgacgcctgc
     4321 ggcccgcacc gcggacggcg aacgaccggt tacggagccc gcgtgaaggt cctggtcatc
     4381 gccgcacacc cggatgacga ggttctcggt gccggggcca cggtgtcggc attgagccag
     4441 cagggcgcag tggtccagat ccacatcctc gccgagggta tctcgctgcg gcattcgggc
     4501 gtgaagatcg aggaggcgcg ggaacgctgc caggtggcgg ccaaggatct cggtgcggcc
     4561 gaggtgtcgt tcggcggtct ggcggccgac ggacgcctcc tggccgacct ccctcagcga
     4621 caggtcgtcg atgcggttag ccacgccctg cgtgaggcag agcccgaagt ggtcttcact
     4681 caccaccctg gcgacatcca cgcggaccac cgcagcgtcg cccatgcagt ggggtacggc
     4741 acccgcatcc tcggctccgg ctccgttcgg caggtgctgc acttcgaggt cctgtcttcc
     4801 acggaacagc aaacgggcct ggtggcgccg ttcaccccca acctgttcta cgacgtcacc
     4861 gggcacgtcg aagccaagtg ccgggcactt gccgcctacc cgtacgagct gtacgacccg
     4921 ccccaccctc gctccctggc ggccgttcgg acgctggcct cataccgggg gacgcaggtg
     4981 ggggtggaag cggctgaggc attcatgatc ggacgagaac tacgcggccc catggcagcg
     5041 gttcccagtg gagttgatgt gcgatgagag tgctcgtgac tgggggtgcc gggtatgtgg
     5101 gctccttcac cgtacggcgc ctccttgcgg ccggccacga ggtcgtggtt gtcgacaacc
     5161 tcagcacggg ccgccgcgaa gcagtccgtg gctgccgcct acacgtggtg gacatcctcg
     5221 atatcgccag catgggcacg gtcttcgagg agttccaccc tgaggccgtg atccacttcg
     5281 cggccctgaa atcctctgaa gagtcgctgc gtgacatcaa cacgtacttc tccgtcaacc
     5341 tgacggggac gcagaacgtg ctcgccctgt gcgcacgcac cggggtggag cgctttgtct
     5401 tctcctcctc gtgcgccgtg tatggcaccc cgcaaatctg cccggtggat gagaccgcgc
     5461 ccgtacggcc cgaaagtccg tacggagaga cgaagtacct gtgcgagcgc gtgatcgcct
     5521 cctacgcgca ggccacaggg atgcgctacg ccaacctgcg gtacttcaac gcggcaggtg
     5581 cggccgacga cgcttccctg ggagagtacg cgggcccggc atcaacccaa ctcctaccgg
     5641 tggccatacg ctcaaccctg ggcctggcgc ccaccctgcg gatcttcggc aacgactacc
     5701 cgacgaccga cgggacggct ctgcgcgact acatccacgt cgaggatctc gcccaggccc
     5761 acgtgcgggt cctggagggt ctgcaaaaag gcagtcagtg cgggccggtg aacctgggcc
     5821 gcggccagcc cgtcagcgtg cgcgagctga tcgatgtctt gcacgacgtc tccgggaagg
     5881 agaccccggt tgccatggcg ccccgccgtc caggggaccc aggtctgtcc tgggccgatc
     5941 cgagtctggc gctgagccga ttcggctggc gagcagaacg cgaccttgac cacatcgttc
     6001 gtacggcgtg gaactggcac accagtaccc ccgcgtcgct ggagtgacat gcacctctac
     6061 ctactgcgcc acgccgagga ccgttccgga aagttcgacc cgcaccagga tcgcctgacc
     6121 gagcgaggac gccgacaggc gcaggaagcg gctcattggc tggctgagcg gcgtcccaca
     6181 gagcttcgca ccagtgtgct tccccgcgcc cagcagacag ccgacatcgt cgcggcgcac
     6241 accggccttg ccgcacggga ggatccgcgc ctcaatgaga tcgtctggtc tccggtcgcg
     6301 ggtgagttcc tgccgaaccc gcgggaggac cggctccatc cgccgccacc gggggcggaa
     6361 tcatggaatg tgttcatgac gcgggtcgcc acatgcatga gcgagctgtg tcatcaggcg
     6421 tcggaggacc agcggattgt gttggtcacc cacagtggct tcttcgacgc tgtcaatgaa
     6481 ctgctgtgtg gcgggaccgg cagggtggag ctgctggttg cgcacgctgg gatcacacac
     6541 tggcagtacc gtcccggaga ctcgggtggc gcctggcttc tgcaccaaca caatgtcacc
     6601 ttcgccccac agaccctgat ggatcacgga tccctgagca cagcggagtt gagcgcatga
     6661 gcgggcggcg caggcgtatg gtcgtcatct gtgtcgacgg cgggcttccc gggatcatcc
     6721 gcgagcacga cttcttcaat ctgagccggg ccctgccgac acttcccggc actgccgtgc
     6781 acgagctgcg cagcatctac ccctcgtcaa cagcgccctc ccacgcgtcc tttcttaccg
     6841 ggacgtaccc gagcgggcac gggatcgtgg ggaaccgctt ctgggagcgg gagagtgttg
     6901 aggagatccg ccgacgctcg gacgatccgc tttcatcttt tcacccctac gaggaaagct
     6961 cgctcaccgc accgagcctg ctcgactggt ttgcccggca aggggcgtcg gccgccgccg
     7021 tccatttccc gcagaccttc tcccgcaacg cccagctcgc gatccccagc tgctactgct
     7081 tgtacgcccc agcccggaac ctcgtcgtcc cgctcggacc gaccgttgac ggggcggccg
     7141 agggcgtggt gcagctctcc tacctgggac acgaggtggc cttgtatctc cgggtcgatc
     7201 agcagacgaa cgtgatcacg gtgggaagcc accgagagac cgctgtcgtg gccgacagcc
     7261 tccggcccac gcggctggac atccccgttt cttcgggttc cgtgtccgtg gcggtctcct
     7321 gccgcaggct cgacgaaggc cagatcgagg tgcgtctggg aaccgccgtt atcaccctgg
     7381 gattcggcgg gctcgatatg ccggaccgcg ccggtgacgg gcccgcgtcc ctgtacgtcg
     7441 aatacaccgc caatccaggc cacacgttcc acgaatcgcc tcgggccgag tgggtggaac
     7501 agaccgccct cgacgtcctg aagcagcatg acccagatgt cctgttcgtg cgcttcaacc
     7561 aggccgacca tgctcaggag ttcctctact ggcatgcggt ccgaggcagc tccggtgaac
     7621 gaagtcgcgc atggcagcag atccttgacg tctacgcccg gatcgacgcc tgcgtgatgc
     7681 ggctcgccca ggccgccggt gagcacgcgg agttcctgtt cttctccgac cacggcatcg
     7741 actacgtcga gacccacctg cgcccgaacc acgtgctggc ggacctcggg ctcgccgacc
     7801 gcatggtttt ccagggtgac agcaactgcg cctacctgta cgccgacgca cctgtgccac
     7861 cagcggagaa aaagcgcctc gaccaggccc tgcacgcact cgatccttcc atcggcgtgc
     7921 tcactccagc cgagcaggac cggttgaagt tgccggtagg gagcccgagg ctgggccgtc
     7981 tcacggtgac ctgcggcccg cacaccgaat tccagtacag cgacgcgaca ccgagggaga
     8041 gcgtggcctc cgcatcacac ggttacctgc ccagcgactc ggccatgagc ggcttcttcc
     8101 gctgcttcgg ccaggcagca gcgcggccgg ctccccccaa ggacatcacg ggggccgcaa
     8161 ccgcggtccg cagcatctgg cgccaagcgg cgcgggagat gtgagcatgc cagaaaaccc
     8221 tccttacgca tatgaggtcg agggcctgca caagcagttc gacggagtcc ccaggccggc
     8281 caatggcgag atctcctttc acataaagca gggcgaattc ttcggacttc tgggcagcaa
     8341 tggtgcggga aagacaaccc tgatcaagca gatgatcggt ctggtgcgac cggatggggg
     8401 gacggtgagg ctactggggc gcgatgtctt tgcccaccct cgcttcacgg cggccacggt
     8461 tggttacatg ccgcagaccg ccttcgccct caacagcctg accgtcaaag aggccgtcta
     8521 cttcagtgcg cacctacgcg gtgtcgccgc caaggcggcc cgccgggagc gtgacgccct
     8581 gttggagctg cttggtctga cccacctggc cggcaaggtc gcccggcagc tctccggcgg
     8641 agaacgccga ctgttgcaga tcgcggtgac gctgatcgga tcaccgcctg tgctgatcct
     8701 ggacgagccc accaacgagc tggacccgtc gcgtcgccgc cagatatggc agcttctcag
     8761 ggagctgaac caacgcgacg gccacacgat catcctcatc acccacaacg ccatcgaagc
     8821 ggaacaggtg atcgagcgcg tcggaatcct caaggagggg cggctcgtcg cgctgggcgc
     8881 tcccgccgag ctgaagtctt ccctccgctc ccaggtacgc ctgtcgatca cggtaggcga
     8941 tacacaaatg catggtttgc ctgactatgt ccgcgtgcag tccgaccacc gcggcaaggt
     9001 cgtggccctc gtcgactctg ccgacctgcc ccatctgacc aaggacgtag atctcaccca
     9061 gttccccgag ttcacgatgc acaccgcgac cctggaagac gtgtatgtca gctacgcgtg
     9121 acaagaagct cggactgagc ttccttgggc aggtctggga cctgttcagg attcagctca
     9181 ccaactggcg gtggtcctgg cctcagatgg tgctcactgg tgtgcttgcc cctttggtca
     9241 gcatcgccgc cttggggctc ttcgcccgga actctggcca cgccgcgacc gaatatgtcc
     9301 tgaccggcag tgtcaccatg gcgatcctgt tcgagactca aaacaaaatc gccagcaact
     9361 tcgcgttcat gcgcggaaac ggcgccttcg agtactacgc cgcactgcct gtccgtcgcg
     9421 aggctctcat ctttgccaca ctcgcagcct tctccatcct gtcgctgcct gccatcgcgg
     9481 tgaccctgct gctcggctcg gccctactcg acgtcccgct cacgttctcg ccactcgccc
     9541 cgctgtgcct cctgcttgcc ctgcttcctt cagccggact gggggccttc atcggcagca
     9601 gatccggaac catcgaacag gcgtcctctc tcagcctggc caccacgctc ctcatgatgg
     9661 ccgcaggccc ggtggcagtc ccaccagaac tgctgcccga cgcactgatc tggatcggca
     9721 acctcaaccc ggcggtctac gcggccgaca gcctgcgtca ctccctaacg gcacccgacg
     9781 cctcccgggc cctttctgac ttggcgatcc ttgccatctt tgccggcgcg gtgtggggcc
     9841 tgaccagcac acgtatgcac tggcgtgccc gctacgggcg ccgcgcctcg tgacgcaacc
     9901 atcgatcact catcacgaag ggaaacacat ccatgaccga gcaacagttc ctcgcagtcc
     9961 tcgcacccct gatcgaggaa atcaccggga acgccttctc ccaggaacaa ctggacgtca
    10021 ccttcggcga tctggacatc gactccctca atcagatcga gatcatcagc cgtatcgagg
    10081 acgagttcga ctgccacata gaggacagca ccctgcggac gatccgtacg ccccgcggac
    10141 tgctggacca catttcgaag tccaccagcc ccgcatgatc cgaaaccact ccggcgaccg
    10201 gccccggtca ctggcatggt tcgcgtggtg ggcagcggtc cacgccggtc tgctactcgt
    10261 cctcgtcacc ttccggtggg acgaacggct gagcgagacg ctctgcgcct ggacgagcag
    10321 cagcgcgtcc agtgcccggc ttctcgagac actgacctcg tggggcaacc cgtggcctgt
    10381 gaccatcatt gccgccggat ccgcgtgctt cgcgctgatc cggcggaacg tcacagctgc
    10441 cgcagtcctc ctggtcattc cgtacgccgc cacagtgagc tgcaacctgc tcaagggatg
    10501 ggctgaacga cagcgtccgg ccttggattg tgcctcgatt caagccgacg gcttcagttt
    10561 tcccagcgga cacgcggtcg gtgtgacgac cggattcatg ctggccgcgc tctacttcag
    10621 ttcccagatg ccacgggccg tctccgcact gatgctgttc cttgctgggc tgagcgccga
    10681 actcgtcgcg tggagcaggg tccttctcgg tgcgcacttc agcgtcgatg tgctcgctgg
    10741 gcaactcttg ggcgccggct ggctggccgt cgccgccctc gtgctgcacc gccagtcagt
    10801 ccgccgcttg caacgcgagg cccgtcaagc ggggtcgaac tccctggacg agcccgtgtc
    10861 atgatgacgc cgtgccgttc aaccacaacg atcattacca tgacctgtta cttcgtgaac
    10921 ttcccgaatc atgccgaagg gcccttgagg tgggctgcgg cacagggagg tttgcacaga
    10981 aactagcgct tcgaggaatc gaagtagatg cgattgacgt agataatgac gtcgtcaccg
    11041 ccgcacgaga gatgaattct actctcgatc tccccaggca gatccgcttc gagcgggcag
    11101 atatcacaca cctaaccttg acacctgaca catacgacta catcgcgtgc ttggccagca
    11161 tccaccacgt cccctttgag acagtgcaga agctgcgcgc atcgctcacc acgaacggcg
    11221 tcttggtgat cctcggatgc taccgagaag caacgatatg ggaccacgcc ataagccttc
    11281 tggccgtccc tgtgaacgct atttggcgtg ctgcggtgtt cttccgagag agctggtcag
    11341 gaagccgtcc cgatcggggc acgcagtctc cttcagcacc tgtggcgccc ccttcgatgt
    11401 cgctcgcgga gatccgagaa gacagtgctg aatatcttcc aggcaggcgg attcggcgcc
    11461 tcctcttctg gagatatctt ctggtctttc acaacatgaa ggcagggctg ggaagtgatt
    11521 agcagccgga cacacatgat ctctcagcca ccgagaagat atgccctggg ggccacctgc
    11581 gttacgttca acgacgtggg acgtgtcctt attgcgcgcc gccgctcccc ggaacgctgg
    11641 gagctgcccg gtggcttggt ggacccgggc gaggcattcc atgatgccgc cactcgcgag
    11701 acctacgagg agaccggggt ccacgtcaaa gtccacggct tggtgggtgt ctaccagcat
    11761 ccgagccggg gcatcctcgc aggcatcttc atcgcgacag ccttgtcggg ggaacctcgc
    11821 ccaacagcgg aatccatcgc cgccgagtgg gcggacgtgg aggatgctct cacgcgactg
    11881 caccccctct atcgaccgcg ccttgaagac gccctcacag ccagggcttc cgtgacgctc
    11941 cgcgtccatg aaggaacgga ggtcctctca ctcttcgagg ccacacacgt ctga
//
