LOCUS       BGC0000881             10499 bp    DNA     linear   BCT 11-NOV-2012
DEFINITION  Streptomyces ahygroscopicus subsp. wuzhouensis strain S91
            toyocamycin biosynthetic gene cluster, complete sequence.
ACCESSION   BGC0000881
VERSION     BGC0000881
KEYWORDS    .
SOURCE      Streptomyces ahygroscopicus subsp. wuzhouensis
  ORGANISM  Streptomyces ahygroscopicus subsp. wuzhouensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
COMMENT     ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2024-10-17 14:22:05
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 10499
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..10499
                     /collection_date="1988"
                     /country="China: Wuzhou town of Guangxi"
                     /db_xref="taxon:387850"
                     /isolation_source="forest soils"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces ahygroscopicus subsp. wuzhouensis"
                     /strain="S91"
                     /sub_species="wuzhouensis"
     misc_feature    1..10499
                     /note="toyocamycin biosynthetic gene cluster"
     subregion       1..10499
                     /aStool="mibig"
                     /contig_edge="True"
                     /label="other"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..10499
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            84..2960
                     /gene="tymA"
     CDS             84..2960
                     /codon_start=1
                     /gene="tymA"
                     /gene_functions="regulatory (smcogs) SMCOG1149:LuxR family
                     transcriptional regulator (Score: 372.8; E-value:
                     6.9e-113)"
                     /gene_kind="regulatory"
                     /note="similar to LuxR"
                     /product="TymA"
                     /protein_id="AFV92621.1"
                     /transl_table=11
                     /translation="MYRTDDGPKTSMTGRQNELRRLGAALEDGAHGGTVRALVTGEPGI
                     GRTTLLQACAAAARARGWITVEVRCPPHEHLPPFGLARHLAGRLAALLPPEHPRRTALD
                     AVAHPPADRTGPADPTPVAADVVLGHVRAALTVLTAGAPVLLGLDDAHRADAASLDFLH
                     RALLAPPLARVVLVASARDGENRSGGGPGTLPELLAGARQLPLTGLAPSQSAALLRRVA
                     RRAPAPDVAAECHRACAGNPALLTEVARVLRERPADRAGAPLAAALRPAAVAELIGTRL
                     AGLDPNAGKVALAIAVAETHSAAEPLLVSHLSGLTLAETLAAVDLLVCMHLVADDDTLY
                     LRHRVVREALLGRMTLMARNAAHLAAATHLHGKQAPADRIADHLAASTVTLDEPWAAPV
                     LLRAGQKALAAREPQRARRYLAHAVRAASGADRRTAVLELVDAEIGIDLQAGLREAVRT
                     LEETTEDALRLPLLARISTARYLGTDPGATARTTPAPDYARPDWHPVHALLDGLAAYPL
                     PDTARRTGERLAPEEPGPGCPETGGADCCGADRAAADAVVRALRSHLTCRPADAVHGAR
                     EALRAEGGLCLHPVLRLMALMILAWNGRDADVTSHLRLHHDCGHCHAHPLHRALLLPVT
                     AHLALLRGRLRTAHDRLGASLDALAHYGTRPQHPLLIWLVGMLAEVRLAMGRGPEARAL
                     LHEHGCLDGPAPDRLHREVWLTRARLRADGGDLAGVAADLAELTDAPGGPTGGVHTYVH
                     TVDLLTQVGRHVQARETARTLLRVAEAMGTPRERGIALRVLGALGGAPEGEDLLREAMR
                     LLAAAEADFDLAHAAADLALLLFDDGRYEEATAELSTALELADRCGARPLALRVRQRLA
                     AAEGRSADRSPLGGVLKLTKREKQILIDAVRGSTNERIATTLHITRRTVELHLSSAYRK
                     LDITGRKDFPHLFRTAGLWPLLVDSAAT"
     gene            3116..3832
                     /gene="tymM"
     CDS             3116..3832
                     /codon_start=1
                     /gene="tymM"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Asn_synthase"
                     /gene_kind="biosynthetic-additional"
                     /note="similar to ExsB"
                     /product="TymM"
                     /protein_id="AFV92622.1"
                     /sec_met_domain="Asn_synthase (E-value: 8.1e-14, bitscore:
                     41.3, seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDQDDSRTDPFTAVVVLSGGLDSTTLMAHYAALQYRLIAVTVDYG
                     QRHHREIDAARAVAAHFGAEHHVVDLRGYGALLRGSALTDDRVSVPDGHYAEQSMRATV
                     VPNRNAVLANVAVSIGVAHRASVVALGMHAGDHFIYPDCRPEFVAALRELVATANEGFA
                     VPQVEAPFLSWAKTDIATHGVRLGAPLELSWSCYKGGDRHCGTCGTCYERREAFRNAGL
                     ADPTGYLDDVTAYPAP"
     gene            complement(3936..4595)
                     /gene="tymI"
     CDS             complement(3936..4595)
                     /codon_start=1
                     /gene="tymI"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1115:HAD-superfamily hydrolase, subfamily IA, variant
                     (Score: 65.4; E-value: 9.8e-20)"
                     /gene_kind="biosynthetic-additional"
                     /note="HAD superfamily protein"
                     /product="TymI"
                     /protein_id="AFV92623.1"
                     /transl_table=11
                     /translation="MLDRDGTLLDFYEMFHRFVLDLHRQEGVIPPPREEILGFGYWSSI
                     TSGALHIGSVRVRDRVDEVAHRYMAHGTLYPGVVPMLSALSGAGVRLALVSSWVGTEAT
                     VALLERYAVRPCFGPILTRDDLDAGDGPVSDADCKTALARRALDGLGHAPDHRLYVVGD
                     TPSDIALGRRLGAVVVGVRTGNGGGLPAEGTPEGPDVLLPSAADLGRLILQAEPVG"
     gene            complement(4640..5146)
                     /gene="tymH"
     CDS             complement(4640..5146)
                     /codon_start=1
                     /gene="tymH"
                     /note="similar to phosphoribosyl-pyrophospate transferase"
                     /product="TymH"
                     /protein_id="AFV92624.1"
                     /transl_table=11
                     /translation="MWRRDRLLTISWEAMGRMLAGLADDVRASGFAPTAVAGIARGGLP
                     PAIALANLLAIEDFRILGIPRNTSNSRYSERGAAVLKYVVPEREFGGARVLLVDDIMGD
                     GGTMALAKRTVEELGAAEVRTVAVVRNLGAAQRADFQAVEVDDWTVFPWEAPPGDNELA
                     VPLRV"
     gene            complement(5158..6213)
                     /gene="tymG"
     CDS             complement(5158..6213)
                     /codon_start=1
                     /gene="tymG"
                     /note="similar to adenylosuccinate synthetase"
                     /product="TymG"
                     /protein_id="AFV92625.1"
                     /transl_table=11
                     /translation="MPGTVIVGGQWGDEAKGKISSYLAIRDEPAVAVRAGLGPGAGHTV
                     VHAGRTMKLRQLPSAVIRPATRLLLGAGVLIRPEVLLREIAEFSAESRVGVDFRATVID
                     PEHVAAEAADAGLTDRVRSTGSGHGPALAARAMRAARRAEDVPELAPYLTDTVRVVNDA
                     VDAGDRVHVEGTNGFGLSVLYGTYPYTVGKDSTASTAAADAGLGPTAIDEVVLVFRAYP
                     TRVGAGPFRTEMTEAEAERLGIVEYGTVTGRRRRAGTFDLDQAREAVRVNRPTRIALTF
                     LDHVDASCRAVRTGELPEAAAAFVARVEEGLGRPVDLLGTGPGTADVVDRWNGRPAGTA
                     RNVTSGQGGDR"
     gene            complement(6198..7583)
                     /gene="tymF"
     CDS             complement(6198..7583)
                     /codon_start=1
                     /gene="tymF"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1063:argininosuccinate lyase/adenylosuccinate lyase
                     (Score: 133.7; E-value: 1.6e-40)"
                     /gene_kind="biosynthetic-additional"
                     /note="similar to adenylosuccinate lyase"
                     /product="TymF"
                     /protein_id="AFV92626.1"
                     /transl_table=11
                     /translation="MTNPWEDGRYGSPEMRLLVSDRNRYQLFVRVEGALAAEQGQLGVI
                     PSEAAGAIAARARDHRCDLARIAEIETVSRHELYAVVQQFAETCAPHGRYVHHGVTSSD
                     VIDTALALQLDAAIGLLGERLGVLMSALCRQIAEEGGRVMIGRTHGQHAQPVTLGFKLA
                     VFLDQLTRCLARLAELRERAVRGKVAGAVGSLGGLGPQGMAVQRAVLARLGLPEPQICA
                     QAVARDRIAEFVSWAALTAACLENLATEIRNLQRTEIGELAESFAEGDQIGSSAMPHKR
                     NPVRSERICSLARLLRSLVGPALENVVTWHERDLANSANERFTLPQACVLLDEMLLTTT
                     AVVDDLRVFPERMRENLDRSRHSHLSEAVLLAMVDRGTDRLDAYRALQRASALAARDDR
                     DLVAVLADDASVTAVLPAPDLAALAEAAADTGFCDTLAQETVRRAEEALAQSTEGARTC
                     PAR"
     gene            complement(7580..8725)
                     /gene="tymE"
     CDS             complement(7580..8725)
                     /codon_start=1
                     /gene="tymE"
                     /note="similar to GMP reductase"
                     /product="TymE"
                     /protein_id="AFV92627.1"
                     /transl_table=11
                     /translation="MAVRDIRTGLSLDDVLLVPRRTSVVSRSATDTSTELAGITLRVPV
                     LSANTPWCTGRQMAVAMAVAGGLGIVHRMQTAEDQAAEVAAVKATPVPEGTERATVDAQ
                     GRLRAGAAVGVTDDYLERAARLVEAGADVLVTDVAHGHADYVLASVEKLRARFPDTPLI
                     AGNVATAAGTRDLIEAGAHAVKVGIGPGGICTTRLVAGSGVPQLTAVLDCAEEAAGSGV
                     PVIADGGIKAPGDVVKALAAGGHAVMLGSALAGADESAARLVERDGVPVKVSTGFVTFG
                     MQLTLKRARGEEVSREELDAYTPEGVEATFAHTGPLAATLRPFVGGLRSGMSYSGAHTL
                     AELRAQAEFIRVTPAGLSESRPHALERTQQVALDYAREATG"
     gene            complement(8760..9365)
                     /gene="tymD"
     CDS             complement(8760..9365)
                     /codon_start=1
                     /gene="tymD"
                     /note="GTP cyclohydrolase I"
                     /product="TymD"
                     /protein_id="AFV92628.1"
                     /transl_table=11
                     /translation="MSTVSGAPELDVLDDSGPQVPDPLEDVARKLLIEIGEDPERDGLK
                     ETPARFARWWREFSRYDAGAVDTLFPLQTEGQLVMVSDINVWSLCEHHLLPFSCSLTIA
                     YRPKGDVLGLSKFARIAHRHAHRLQVQERLVRDIADEVTKVTGSVDVAVIATGEHLCMS
                     MRGIRTPAVMTSTAYSGVFEEYGPARQELVATVFPLRK"
     gene            complement(9362..9991)
                     /gene="tymC"
     CDS             complement(9362..9991)
                     /codon_start=1
                     /gene="tymC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_12"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF04055"
                     /gene_kind="biosynthetic-additional"
                     /note="similar to radical SAM"
                     /product="ToyC"
                     /protein_id="AFV92630.1"
                     /sec_met_domain="Fer4_12 (E-value: 1.8e-11, bitscore: 34.0,
                     seeds: 84, tool: rule-based-clusters)"
                     /sec_met_domain="PF04055 (E-value: 2.6e-13, bitscore: 40.1,
                     seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRLGGCNLSCRWCDTPYTWDWTGVGDSGVAYDPKRELRPMPWTDV
                     LRALLAYEVPLIVVSGGEPLSQQRRLLPLLGALTEAGRKVEIETNGTVVPQDDLVALGV
                     RFNVSPKLSHSGDPQARRIVPDALRAFAAVPGTAFKFVCRTTSDLDEVAQLVDRYGTRP
                     VWIMPEGQVPAEVGQHLSALGDDVVARGWNLTTRLHVAVWGDRRGV"
     gene            complement(10131..10493)
                     /gene="tymB"
     CDS             complement(10131..10493)
                     /codon_start=1
                     /gene="tymB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     nucleoside: ToyB"
                     /gene_kind="biosynthetic"
                     /note="similar to 6-pyruvoyltetrahydropterin synthase"
                     /product="TymB"
                     /protein_id="AFV92629.1"
                     /sec_met_domain="ToyB (E-value: 1.5e-64, bitscore: 205.2,
                     seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAFRITKEFHFSASHRLAELPEGHPCGRMHGHNYVVELELAAPAD
                     GLDKAGFVRDYGDLKQFSRWLDAEVDHRHLNDVLDRNPSAEHLAQWLYERWLPHYPQLS
                     AVRVSETAKTWAEYRP"
ORIGIN
        1 gccaacccgt tcggcgggcg gccgcaccga gaagagagac ggtgctactc gggtagagcg
       61 gtgagccgtg acaggggaac tccatgtatc gcacagacga cggaccgaag acgtccatga
      121 cggggcgtca gaacgagttg cgccggctcg gcgcggccct ggaagacggc gcgcacggcg
      181 ggaccgtccg cgccctggtc accggcgaac cgggcatcgg ccggaccacc ctgctccagg
      241 cgtgcgcggc cgcggcccgc gcccggggct ggatcaccgt cgaggtgcgg tgcccgccgc
      301 acgaacacct gccgccgttc ggcctcgccc ggcacctcgc gggccgactg gccgcgttgc
      361 tgccgccgga gcacccccgc cgcaccgcgc tcgacgccgt cgcgcacccg ccggccgacc
      421 gcaccggacc ggccgacccg acgccggtgg cggcggacgt ggtgctgggc cacgtcaggg
      481 cggccctcac cgtgctgacc gccggcgcac ccgtgctcct cggcctcgac gacgcccacc
      541 gggccgacgc cgcctcgctg gacttcctgc accgggccct gctcgccccg ccgctcgccc
      601 gcgtcgtcct ggtcgcctcc gcccgggacg gcgagaaccg cagcggcggc ggccccggca
      661 cactgcccga actcctcgcc ggcgcacggc aactgccgct caccgggctg gccccgtccc
      721 agtccgcggc gttgctccgc agggtcgccc gccgggcacc ggcccccgac gtcgccgcgg
      781 agtgccaccg cgcctgtgcc ggcaaccccg ccctcctcac cgaagtcgcc cgggtgctgc
      841 gcgagcggcc ggcggatcgc gccggtgcgc cgctggccgc cgcactgcgg cccgcggccg
      901 tcgcggaact gatcggcacc cggctggccg gactcgatcc gaacgccggc aaggtcgccc
      961 tggccatcgc cgtcgcggag acccactccg cagccgagcc cctgctcgtc tcccacctca
     1021 gcgggctgac cctggccgaa accctggccg cggtcgacct gttggtgtgc atgcacctgg
     1081 tcgccgacga cgacacgctg tacctgcgcc accgcgtcgt gcgcgaggcg ctgctgggcc
     1141 ggatgacgct gatggcccgc aacgccgccc acctcgcggc cgccacccac ctgcacggca
     1201 agcaggcgcc cgccgaccgg atcgccgacc acctcgcggc gtcgacggtg accctggacg
     1261 agccgtgggc cgcccccgtg ctgctgcggg ccgggcagaa ggccctcgcg gcccgggaac
     1321 cgcaacgggc ccggcgctat ctggcgcacg ccgtacgcgc ggcgtcgggc gcggaccgcc
     1381 ggaccgcggt gctggagctc gtcgacgccg agatcggcat cgacctgcaa gcggggctgc
     1441 gggaggcggt ccggaccctg gaggagacca ccgaggacgc cctccggctc ccgctgctgg
     1501 ccaggatcag caccgcgcgc tacctcggaa ccgaccccgg cgcgaccgcc cggacgacgc
     1561 ccgccccgga ctacgcccgg cccgactggc atcccgtgca cgccctgctc gacggcctgg
     1621 ccgcctaccc gctgccggac accgcccgac gcaccgggga gcgcctcgcg cccgaggagc
     1681 ccgggccggg gtgccccgag accggcgggg ccgactgctg cggcgcggac cgggccgccg
     1741 cagacgccgt cgtgcgcgcc ctgcgcagcc acctgacgtg tcggccggcc gacgcggtgc
     1801 acggcgcgcg ggaggcgttg cgggcggagg gcggactgtg cctgcacccg gtgctccggc
     1861 tgatggcgct gatgatcctc gcctggaacg gccgcgacgc cgacgtgacg tcccacctgc
     1921 ggctccacca cgactgcggg cactgccacg cgcacccgct gcaccgggcc ctgctgctgc
     1981 cggtcaccgc ccacctggcc ctgctgcgcg gccggctgcg caccgcgcac gaccggctcg
     2041 gcgcatccct cgacgccctg gctcactacg ggacccggcc ccaacacccg ctgctgatct
     2101 ggctcgtggg gatgctggcc gaggtccgcc tggccatggg acgcggcccc gaggcccgcg
     2161 cgctgctgca cgaacacggc tgcctggacg gcccggcacc ggaccgactg caccgcgagg
     2221 tgtggctgac ccgggcgagg ctgcgggccg acggcggtga cctggcgggc gtagcggcgg
     2281 acctggccga gctcaccgac gccccggggg gacccaccgg cggcgtgcac acctacgtcc
     2341 acaccgtcga tctgctgacg caggtgggcc ggcacgtgca ggcccgggag accgcccgga
     2401 cgctgctgcg ggtcgccgag gccatgggca caccgcggga acgcggcatc gccctgcggg
     2461 tgctcggcgc actgggcggc gcccccgagg gcgaggacct gctgcgcgag gcgatgcgcc
     2521 tgctggccgc ggcggaggcc gacttcgacc tcgcgcacgc cgccgccgac ctggccctgt
     2581 tgctgttcga cgacgggcgc tacgaggagg cgacggcgga gctgagcacg gcgctggagc
     2641 tggcggaccg gtgcggcgcc cgaccgctcg cgctgcgggt gcggcagcgg ctcgccgccg
     2701 cggaggggcg ctccgccgac cggtcgccgc tgggcggcgt gctgaagctg accaagcggg
     2761 agaagcagat cctcatcgac gccgtgcgcg gctcgaccaa cgaacggatc gcgacgaccc
     2821 tgcacatcac ccgccgcacg gtcgaactcc acctgtccag tgcgtaccgc aagctcgaca
     2881 tcaccggccg caaggacttc ccgcacctgt tccggaccgc ggggctgtgg ccgctgctgg
     2941 tcgattccgc cgccacctga ccggcgcgcg ggacccaccc ctcggtaccg gggcgcccgc
     3001 gctacggcac cggccgtgat gttccgcggc ccggccggcc gccacgatgg ccccgggcgc
     3061 cgggccgccc cgcgtcccgg tcgttcggtt tcccttccca ctccggaggt ttgtgatgga
     3121 tcaagacgac agccgcaccg acccgttcac cgccgtggtg gtgctgtccg gaggtctgga
     3181 ctccacgacg ctgatggcgc actacgccgc cctccagtac cggctgatcg ccgtcaccgt
     3241 cgactacggg cagcgccacc accgcgagat cgacgcggcc cgggcggtgg cggcccactt
     3301 cggcgccgag caccacgtgg tcgacctgcg cggttacggg gcgctgctgc gcggctccgc
     3361 actgaccgac gaccgggtca gcgtccccga cgggcactac gcggaacagt ccatgcgggc
     3421 caccgtcgtg cccaaccgca acgcggtgct ggcgaacgtc gcggtgtcga tcggggtggc
     3481 gcaccgcgcg tcggtggtgg cgctgggcat gcacgccggc gaccacttca tctacccgga
     3541 ctgccggccg gagttcgtcg ccgcgctgcg cgaactggtc gcgacggcca acgagggctt
     3601 cgcggtcccc caggtcgagg cgccgttcct ctcctgggcc aagaccgaca tcgccacgca
     3661 cggcgtccgg ttgggcgccc cgctggaact cagctggtcc tgctacaagg gcggggaccg
     3721 gcactgcggg acgtgcggga cctgctacga gcggcgggag gcgttccgca acgccgggtt
     3781 ggccgacccg accggctacc tcgacgacgt gacggcgtac ccggcgccct gacgccccgg
     3841 gcgcgcccgg ccccgcccac gggccgagca ccgcgacggg ccggactccg tgcggagtcc
     3901 ggcccgtcgg cacgccctcg gcgggcggaa ggggctcacc ccaccggttc cgcctggagg
     3961 atcaagcggc ccaggtccgc ggccgacggg agcaggacgt ccggcccctc cggcgtgccc
     4021 tcggccggca gcccgccgcc gttgccggtg cgcaccccga ccacgacggc ccccaaccgg
     4081 cggcccaacg cgatgtccga cggggtgtcg ccgacgacgt agagccggtg gtcgggcgcg
     4141 tggccgagcc cgtccaacgc ccgccgcgcc aacgcggtct tgcagtcggc gtcggagacc
     4201 gggccgtccc ccgcgtccag gtcgtcccgg gtcaggatcg ggccgaagca cgggcggacc
     4261 gcgtagcgct ccagcagggc cacggtggcc tcggtgccga cccaggagct gaccagcgcg
     4321 agccgcaccc cggccccgga cagcgccgac agcatcggca ccaccccggg gtagagcgtg
     4381 ccgtgcgcca tgtaccggtg ggccacctcg tcgacccggt cccgcacccg caccgacccg
     4441 atgtgcagcg ccccgctggt gatcgaggac cagtagccga agccgaggat ctcctcgcgg
     4501 ggcggcggaa tcaccccctc ctgccggtgg aggtccagga cgaagcggtg gaacatctcg
     4561 tagaagtcca acagggtgcc gtcccggtcc agcacgaccg tggtccgccc tgtccgaccc
     4621 atttcctcaa ccatgtcgct cacacccgca acggtacggc gagttcgttg tcgccgggcg
     4681 gtgcttccca cgggaagacc gtccagtcgt ccacctcgac cgcctggaag tcggcccgtt
     4741 gggcggcgcc caggttgcgc accaccgcga ccgtgcggac ctccgcggcg ccgagctcct
     4801 ccacggtgcg cttggccagg gccatggtgc cgccgtcgcc catgatgtcg tcgaccagca
     4861 gcacccgcgc gccgccgaac tcccgctccg ggacgacgta cttgagcacc gccgcccccc
     4921 gctcgctgta ccgggagttg gaggtgttgc gggggatgcc caggatgcgg aagtcctcga
     4981 tcgccagcag gttcgccagg gcgatggccg gcggcagccc gccgcgggcg atgcccgcca
     5041 ccgcggtcgg ggcgaagccg ctggcccgga cgtcgtccgc cagtccggcc agcatccggc
     5101 ccatcgcctc ccaactgatc gtcaacagcc ggtcccgccg ccacatcggt gtgccgctca
     5161 tcggtcgcct ccctggccgc tcgtcacgtt gcgggccgtg ccggcgggcc ggccgttcca
     5221 ccggtccacc acgtcggcgg tgcccggccc cgtcccgagc aggtcgaccg gccggcccag
     5281 gccctcctcg acccgggcca cgaacgccgc cgccgcctcg ggcaactccc cggtgcgcac
     5341 cgcgcggcag gacgcgtcca cgtggtccag gaaggtcagc gcgatccggg tgggccggtt
     5401 gacgcgcacc gcctcgcgtg cctggtccag gtcgaacgtg ccggcccggc gccgccgccc
     5461 ggtcaccgtg ccgtactcga cgatgcccag ccgctcggcc tccgcctcgg tcatctcggt
     5521 gcggaacggc cccgcgccca cccgggtggg gtaggcccgg aagaccagca ccacctcgtc
     5581 gatcgcggtg gggcccagtc cggcgtccgc cgccgcggtg ctcgccgtgc tgtccttgcc
     5641 gacggtgtag ggataggtgc cgtacagcac cgacaggccg aagccgttgg tgccttcgac
     5701 gtgcacccgg tccccggcgt cgaccgcgtc gttcaccacc cgcacggtgt ccgtgaggta
     5761 gggggcgagt tccgggacgt cctcggcgcg gcgcgccgca cgcatggcgc gggcggccag
     5821 ggccgggccg tggccgctgc cggtgctgcg cacccggtcg gtcagccccg cgtcggccgc
     5881 ctccgcggcg acgtgctcgg ggtcgatgac ggtggcgcgg aagtccacgc cgacccggga
     5941 ctcggcggag aactccgcga tctcccgcaa caggacctcc gggcggatca acacgccggc
     6001 gcccagcagg aggcgggtgg ccgggcggat caccgcactg ggcaactggc gcagtttcat
     6061 ggtgcgtccg gcgtgcacca cggtgtggcc ggcgccgggc cccagtccgg cgcgcaccgc
     6121 caccgcgggc tcgtcccgga tcgccagata gctggagatc ttccccttgg cctcgtcgcc
     6181 ccactggcca ccgacgatca ccgtgccggg catgtccgag ctccttccgt cgactgtgcg
     6241 agcgcttcct cggcccggcg cacggtctcc tgcgccaggg tgtcgcagaa gccggtgtcc
     6301 gcggccgcct cggccagggc cgcgaggtcc ggtgcgggca gcaccgcggt caccgacgcg
     6361 tcgtccgcca gcaccgccac caggtcgcgg tcgtcccggg cggcgagcgc actggcccgt
     6421 tgcagggccc ggtaggcgtc gagccggtcc gtcccgcggt cgaccatggc cagcagcacc
     6481 gcctcgctga ggtgactgtg gcgcgaccgg tcgaggttct cccgcatccg ctcggggaag
     6541 accctcaggt cgtccaccac ggcggtggtg gtgaggagca tctcgtcgag cagcacgcac
     6601 gcctgcggca gggtgaagcg ttcgttggcg gagttggcca ggtcccgttc gtgccaggtc
     6661 accacgttct ccaacgcggg gccgaccagc gaccgcagca gccgggccag gctgcagatg
     6721 cgttcgctgc gcaccgggtt gcgcttgtgc ggcatggcgg agctgccgat ctggtccccc
     6781 tcggcgaagg actcggcgag ttcgccgatc tccgtccgct ggaggttgcg gatctcggtc
     6841 gcgaggttct ccaggcaggc cgcggtcagc gcggcccagg agacgaactc cgcgatccgg
     6901 tcccgcgcca ccgcctgggc gcagatctgc ggctccggca ggcccagccg ggccagcacc
     6961 gcccgctgca ccgccatgcc ctgcggtccc agtccgccca gcgaaccgac cgcaccggcg
     7021 accttgcccc gcacggcgcg ctcgcgcagt tccgccagcc gcgccaggca ccgggtcaac
     7081 tggtcgagga acaccgcgag tttgaacccc agggtgacgg gctgggcgtg ctggccgtgg
     7141 gtccgtccga tcatcacccg cccgccctcc tcggcgatct gccggcacag cgcggacatc
     7201 aggacgccga gccgttcgcc caggaggccg atcgccgcgt ccagttggag cgccagcgcg
     7261 gtgtcgatga cgtcgctgct ggtcaccccg tggtgcacgt agcggccgtg gggcgcgcag
     7321 gtctcggcga actgctggac tacggcgtag agctcgtgcc ggctcaccgt ctcgatctcg
     7381 gcgatccgcg ccaggtcgca ccggtggtcg cgggcccggg cggcgatcgc cccggcggcc
     7441 tcggacggga tcacccccaa ctggccctgc tccgcggcca acgccccctc cacccgtacg
     7501 aagagctggt agcggttgcg gtcgctgacc agcaggcgca tctccgggct gccgtagcgg
     7561 ccgtcctccc aagggttcgt catccggtgg cctcccgggc gtagtccagg gccacctgct
     7621 gggtccgctc cagggcgtgc gggcggctct ccgacagtcc ggccggcgtg acccggatga
     7681 actcggcctg cgcccgcagt tcggccaggg tgtgcgcccc ggagtagctc atgcccgacc
     7741 gcagtccgcc gacgaacggc cgcagggtgg cggccagcgg cccggtgtgg gcgaaggtgg
     7801 cctcgacgcc ctcgggggtg tacgcgtcca gctcctcgcg gctgacctct tcgccgcggg
     7861 cccgcttcag ggtcagctgc atgccgaacg tcacgaagcc ggtgctgacc ttcacgggga
     7921 cgccgtcgcg ttccaccagg cgggccgcgc tctcgtcggc gccggccagg gcgctgccca
     7981 gcatcaccgc gtggccgccg gcggccagcg ccttgacgac gtcgcccggc gccttgatcc
     8041 cgccgtccgc gatcaccggg acgccggaac cggccgcctc ctcggcgcag tcgaggaccg
     8101 cggtcagctg cggcaccccg ctgcccgcga cgagccgggt ggtgcagatg ccgccggggc
     8161 cgatgcccac cttcacggcg tgggcgccgg cctcgatcag gtccctcgtg ccggcggcgg
     8221 tggcgacgtt cccggcgatc agcggcgtgt cagggaaacg ggcccgcagc ttctcgacgg
     8281 aggccaggac gtagtccgcg tggccgtggg cgacgtcggt gaccaggacg tccgcgcccg
     8341 cctcgacgag ccgggcggcc cgctccagat agtcgtcggt cacccccacg gccgccccgg
     8401 cgcgcagccg gccctgcgcg tcgacggtgg cccgctccgt gccctcgggc accggcgtcg
     8461 ccttgaccgc ggccacctcc gcggcctggt cctcggcggt ctgcatgcgg tggacgatcc
     8521 cgagcccacc ggccaccgcc atggcgacgg ccatctgccg gccggtgcac cagggggtgt
     8581 tggcggagag gaccggcacc cgcagggtga tcccggccag ctcggtgctg gtgtcggtcg
     8641 ccgagcggct caccaccgag gtgcggcggg gcaccagcag gacgtcgtcc aggctgagcc
     8701 cggtgcgtat gtcgcgaact gccacgtgcg tactcctgtc gatcgtggac ggggtcgggt
     8761 cacttgcgca gcgggaacac cgtcgccacg agctcttgac gggccggccc gtactcctcg
     8821 aagaccccgg agtaggccgt ggacgtcatg accgccgggg tacggatgcc gcgcatgctc
     8881 atgcacaggt gctcgccggt ggcgatgacc gcgacgtcga ccgaaccggt caccttggtg
     8941 acctcgtccg cgatgtcgcg gaccaggcgc tcctgcacct ggagccggtg cgcgtggcga
     9001 tgggcgatcc gggcgaactt cgacagtccg agcacgtcgc ccttgggccg gtaggcgatg
     9061 gtcagggagc aggagaaggg cagcaggtgg tgctcgcaca gcgaccacac gttgatgtcc
     9121 gagaccatga ccagttgtcc ctccgtctgg agggggaaca gcgtgtcgac cgcccccgcg
     9181 tcgtagcggg agaactcccg ccaccagcgg gcgaagcggg ccggcgtctc cttgagcccg
     9241 tcgcgctccg ggtcctcgcc gatctcgatc agcagcttgc gcgcgacgtc ctccagcggg
     9301 tccgggacct gcggcccgga gtcgtcgagg acgtccagtt cgggggctcc gctcactgtg
     9361 ctcacacgcc tctcctgtcg ccccagacgg caacatgcaa tcgagtggtc aggttccagc
     9421 cgcgtgccac gacgtcgtcc cccagcgcgg acaggtgttg gccgacctcg gcgggtacct
     9481 gcccctcggg catgatccac accgggcggg tcccgtaccg gtcgacgagc tgcgccacct
     9541 cgtcgaggtc gctcgtggtg cggcagacga acttgaaggc ggtccccggg acggccgcga
     9601 acgcccgcaa cgcgtccggc acgatccgcc gcgcctgcgg atcgcccgag tgcgacagct
     9661 tgggcgagac gttgaaccgc acgccgagcg ccaccaggtc gtcctgcggc accacggtgc
     9721 cgttcgtctc gatctccacc ttgcggccgg cctccgtcag cgcgcccagc agcggcagca
     9781 accggcgctg ctggctgagg ggttcgcccc cggagaccac gatcagcggt acctcgtagg
     9841 cgagcagcgc gcgcagcacg tcggtccacg gcatcggccg cagttcccgc ttggggtcgt
     9901 aggcgacccc ggagtcgccc actccggtcc agtcccaggt gtacggggtg tcgcaccagc
     9961 ggcaggacag gttgcagccg cccaaccgca cgaaggcgca ccgctgcccg gccgaggggc
    10021 cctctccctg cacggccctc aagtggggcc gaagatctcg ttgacgacga gcttcggttc
    10081 caccgcggca ccacctcctc ggtgttgcga cggggtggcg ggcatctgcg tcacggccgg
    10141 tactccgccc acgtcttggc ggtctcggag acccgcaccg cgctcaactg cgggtagtgc
    10201 ggcagccacc gctcgtacag ccactgcgcg aggtgctcgg ccgaggggtt gcggtccagc
    10261 acgtcgttga ggtgacggtg gtccacctcg gcgtccaacc accggctgaa ctgcttgagg
    10321 tcgccgtagt cgcggacgaa gcccgccttg tccaggccgt cggccggggc cgcgagctcc
    10381 agttcgacga cgtagttgtg cccgtgcatc cggccgcacg ggtgcccttc gggcagctcg
    10441 gcgagccggt gactggccga gaagtggaac tctttggtga tccgaaaagc catgcagac
//
