LOCUS       BGC0000884              3005 bp    DNA     linear   BCT 12-APR-2010
DEFINITION  Pseudomonas syringae pv. syringae strain 22d/93 amino acid exporter
            protein (mrsC), aminotransferase subfamily I gamma (mrsB), and
            methyltransferase type 12 (mrsA) genes, complete cds.
ACCESSION   BGC0000884
VERSION     BGC0000884
KEYWORDS    .
SOURCE      Pseudomonas syringae pv. syringae
  ORGANISM  Pseudomonas syringae pv. syringae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas; Pseudomonas syringae.
COMMENT     ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2024-10-17 14:22:21
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3005
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     gene            complement(1..633)
                     /gene="mrsC"
     CDS             complement(1..633)
                     /codon_start=1
                     /experiment="transfer of the whole gene cluster into E.
                     coli resulted in the heterologous production of
                     3-methylarginine"
                     /gene="mrsC"
                     /gene_functions="transport (smcogs)
                     SMCOG1116:Homoserine/threonine efflux protein (Score:
                     147.4; E-value: 6.9e-45)"
                     /gene_kind="transport"
                     /note="member of the RhtB, LysE superfamily"
                     /product="amino acid exporter protein"
                     /protein_id="ACY54551.1"
                     /transl_table=11
                     /translation="MNVHLYLSFILTSLIVLVVPGPSFAYAIAVGMRSSRQVICRNALG
                     MALGGLTITMALAFGVAQLFAAYPAAFVALKFIGCAYLVALGVRTFLTRPEDKHACTIE
                     TSATPLLQGFIVETANPKAILFYVSLVPQFADPELGHMEAQLLILGATFVALQVTWDIA
                     LMLGVHRLGAITGGLMSMKAQRIINRISGTTFIVLGLALLTQERPNS"
     source          1..3005
                     /collection_date="1993"
                     /country="Germany"
                     /db_xref="taxon:321"
                     /host="Glycine max"
                     /mol_type="genomic DNA"
                     /note="pathovar: syringae"
                     /organism="Pseudomonas syringae pv. syringae"
                     /strain="22d/93"
     subregion       1..3005
                     /aStool="mibig"
                     /contig_edge="True"
                     /label="other"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..3005
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            complement(697..1860)
                     /gene="mrsB"
     CDS             complement(697..1860)
                     /NRPS_PKS="Domain: Aminotran_1_2 (55-304). E-value:
                     1.2e-26. Score: 85.5. Matches aSDomain:
                     nrpspksdomains_mrsB_Aminotran_1_2.1"
                     /codon_start=1
                     /experiment="transfer of the whole gene cluster into E.
                     coli resulted in the heterologous production of
                     3-methylarginine"
                     /gene="mrsB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1019:aminotransferase (Score: 147; E-value: 1.2e-44)"
                     /gene_kind="biosynthetic-additional"
                     /product="aminotransferase subfamily I gamma"
                     /protein_id="ACY54550.1"
                     /sec_met_domain="Aminotran_1_2 (E-value: 6.4e-28, bitscore:
                     85.5, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTLAYNKRHQIVRPDSCLSVLQQRLKYARSELQESACYFVENVPV
                     WPGAAAPTPFAASTDIFQHADCNGAPPLINTICARDNALYGLDLSDENFLVTNGGMHGL
                     SLVFRQIKRRSAEAGSAVCLAPVFGAVPALLEATGYDLIYYQMSAAFPTVEEILAVCRA
                     DTRLVYLNFPHNPLGGIYSDAFIEQLVPSLAERGISLVLDMVYDSFIFDDQKIRSPFAC
                     TNDLSLLYTVNSVSKNYGSPGLRIGWVASSSDNVEAMAGMLEIECVAVCSPAQTKAASL
                     ISMGNAPLHQQVVSSRAIVREFLGRHLSRYASLPPAGTQVFVNLPVHDIEGFADQMLGE
                     YGLVLATASNYSGAQGAHIRIPTGYPESITHNALELLRQGIERYADV"
     aSDomain        complement(949..1695)
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_mrsB_Aminotran_1_2.1"
                     /evalue="1.20E-26"
                     /label="mrsB_Aminotran_1_2.1"
                     /locus_tag="mrsB"
                     /protein_end="304"
                     /protein_start="55"
                     /score="85.5"
                     /tool="antismash"
                     /translation="AASTDIFQHADCNGAPPLINTICARDNALYGLDLSDENFLVTNGG
                     MHGLSLVFRQIKRRSAEAGSAVCLAPVFGAVPALLEATGYDLIYYQMSAAFPTVEEILA
                     VCRADTRLVYLNFPHNPLGGIYSDAFIEQLVPSLAERGISLVLDMVYDSFIFDDQKIRS
                     PFACTNDLSLLYTVNSVSKNYGSPGLRIGWVASSSDNVEAMAGMLEIECVAVCSPAQTK
                     AASLISMGNAPLHQQVVSSRAIVREFL"
     gene            complement(1857..2909)
                     /gene="mrsA"
     CDS             complement(1857..2909)
                     /codon_start=1
                     /experiment="transfer of the whole gene cluster into E.
                     coli resulted in the heterologous production of
                     3-methylarginine"
                     /function="transfers the methyl group from SAM onto
                     5-guanidino-2-oxo-pentanoic acid to yield
                     5-guanidino-3-methyl-2-oxo-pentanoic acid"
                     /gene="mrsA"
                     /product="methyltransferase type 12"
                     /protein_id="ACY54549.1"
                     /transl_table=11
                     /translation="MNLLDSIKSENTGFETTLIKGIEPIRQFVLAISIYHLFDTKLFSL
                     LIKHEVASPEVACNELGMEKEKLLGLFRYLKNEGILLETIDGFSLSKEGHALAPFEGWY
                     VMLVGGYATTFLQMGERLQEGAGWATRDATKVGVGSCGISHFDAIPLTRSLMAQAPGTC
                     TKLLDLGCGNGRYLAEFCKALPQIQAWGAEPDRGGFEEAVDLIEKEGLSHRVHISHSGA
                     VEFLDSDFDFEPDFIVLGFVLHEILGQAGRPAVVNFLKKIVHRFPAINLIIIEVDNQFD
                     NAGAMRHGLALAYYNPYYLLHCFTNQLLVQDADWLDIFAEAGLSLVTRETTSDQVDSTG
                     LEIGYLLRRA"
ORIGIN
        1 ttagctattg ggtcgttcct gagtgagcag agccaggccc aacacgataa aagtggtccc
       61 gcttattcga ttgatgattc gctgagcctt catggacata agacctcctg taatcgcacc
      121 gagccgatgt acacccagca tcaaagcgat atcccatgtg acctgtagag cgacgaaggt
      181 agccccaaga atcagcagtt gagcttccat gtgaccgagc tcaggatcag cgaactgagg
      241 cacgagcgaa acgtaaaaca atatagcctt ggggttcgcg gtttcgacga tgaacccttg
      301 taaaagcggt gttgctgacg tttctattgt gcaggcgtgt ttgtcctcgg ggcgggtaag
      361 gaacgtgcga accccaagtg cgacaaggta tgcgcacccg ataaacttca gagccacgaa
      421 tgcggccgga taggctgcaa agagttgggc tactccgaac gcgagcgcca tggtgattgt
      481 cagcccgccg agcgccatgc caagtgcgtt gcggcaaatc acttgtcgag agctgcgcat
      541 cccaacggcg attgcataag caaaactggg ccctggaacg acaaggacga taaggctagt
      601 gaggataaaa gaaagataca gatgtacatt cattgagtaa gttttcaata catcgagggt
      661 tcagcccgca ggatgcgggc catatttaat ttttattcag acatcggcgt agcgttcgat
      721 gccctggcga agcagctcaa gggcattgtg agtgatcgat tccgggtagc cggtcgggat
      781 acggatatgc gcaccttggg cgcctgaata gttgctcgca gtagccagca ccaggccgta
      841 ctcgcccaac atttgatcgg cgaaaccctc gatgtcatgg actggcaggt tgacgaacac
      901 ctgggtgccc gcaggtggca gcgatgcgta cctcgacagg tggcggccga ggaactctcg
      961 aacgatggcc cggctgctga ccacttgttg atgcagcggc gcgtttccca tcgagatcaa
     1021 ggacgcggcc ttggtttgcg caggcgagca gacggcgaca cactcaatct ccagcatccc
     1081 cgccatcgcc tcgacattgt ccgaagagga ggctacccag ccgatccgca aaccaggcga
     1141 gccatagttc ttcgacactg aattaacggt gtagagcaac gaaagatcgt ttgtgcaagc
     1201 gaagggtgag cgtatcttct ggtcgtcgaa gatgaaggag tcgtacacca tatccagcac
     1261 cagcgagatg ccgcgctcgg ccagactcgg caccagctgc tctataaagg cgtctgagta
     1321 aatcccaccc aatggattat gtggaaagtt gaggtacacc agacgggtat ctgcgcggca
     1381 taccgccagg atctcttcga cggtagggaa ggcagcactc atctgatagt agatcagatc
     1441 gtagcccgtt gcttccagca gggcggggac ggcaccgaat accggcgcca ggcacaccgc
     1501 tgagccagcc tcggcgctac ggcgcttgat ctggcgaaac acaagcgaca agccgtgcat
     1561 tccgccattg gtgacgagga aattctcgtc tgacaaatcc agcccgtaca gcgcgttgtc
     1621 gcgggcgcag atggtattga tcaatggtgg ggcaccattg cagtcggcgt gttgaaagat
     1681 atcggtgctg gcggcaaacg gagtcggtgc tgccgcacct ggccataccg gaacattctc
     1741 cacgaagtag caagccgact cctgcagctc cgaacgagcg tatttcaggc gctgctgaag
     1801 cacgctcagg cagctatctg gccggacgat ctggtggcgt ttgttgtaag ccaaggtcat
     1861 gccctccgca gcagatagcc gatttccaga ccggtcgagt caacctggtc agacgtcgtc
     1921 tcgcgggtga ccagggacaa gccagcctcg gcaaaaatat ccagccaatc cgcgtcctgc
     1981 accaacaatt gattggtgaa gcaatgcaac agatagtaag ggttgtagta agcgagcgct
     2041 aagccgtggc gcattgcgcc ggcattgtcg aattggttgt cgacttcgat aatgatcagg
     2101 ttgatggcag ggaaacggtg gacaatcttt ttcaggaagt taaccactgc cggtcggccg
     2161 gcctggccaa ggatctcatg cagcacaaaa ccaagcacga tgaaatcggg ctcgaaatcg
     2221 aaatccgaat ccaggaattc gactgccccg ctgtggctga tgtgaactcg atgcgacaga
     2281 ccttctttct cgatcaggtc tacagcctcc tcgaagcctc cgcggtccgg ttcggcgccc
     2341 caggcctgga tctgcggaag cgccttgcag aattcggcga ggtatcgacc gtttccgcaa
     2401 cccaggtcca gcagttttgt gcatgtaccg ggagcttggg ccatcaaaga cctggtcaag
     2461 gggatagcgt caaagtggct gataccacaa ctacctacac cgaccttggt tgcgtcgcgg
     2521 gtggcccaac cggcgccctc ttgaaggcgt tcgcccattt gtaggaaggt tgtggcatag
     2581 ccaccgacca gcatcacata ccagccctcg aaaggtgcca gcgcgtgccc ttctttcgat
     2641 aacgagaaac cgtctattgt ttccagaaga atcccttcgt tcttcaggta cctgaataag
     2701 cccagtaact tctctttctc cattccgagt tcattacagg ccacctccgg cgaggcgacc
     2761 tcgtgtttaa tcagcaagga aaataatttt gtatcgaaga gatgataaat cgagatagcc
     2821 agaacaaact gtcgaatcgg ttcaataccc ttaataaggg ttgtctcaaa gccagtattt
     2881 tcgcttttta tagagtcaag cagattcatt gtatgcaatg tccatttgtt tattgattaa
     2941 tccgttaatg tttatatttt tacacaaaat acaaaaaggg tgtcaattat tttctcgcaa
     3001 aaatt
//
