LOCUS       BGC0000888              4342 bp    DNA     linear   BCT 24-JUL-2016
DEFINITION  Bacillus sp. CS93 bacilysin biosynthetic gene cluster, partial
            sequence.
ACCESSION   BGC0000888
VERSION     BGC0000888
KEYWORDS    .
SOURCE      Bacillus sp. CS93
  ORGANISM  Bacillus sp. CS93
            Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
COMMENT     ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2022-11-07 20:57:22
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4342
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     gene            <1..501
                     /gene="bacA"
     CDS             <1..501
                     /codon_start=1
                     /gene="bacA"
                     /note="anticapsin synthesis"
                     /product="BacA"
                     /protein_id="ACX35426.1"
                     /transl_table=11
                     /translation="NFITNFTLLQGVNSKLSLHDTFESCIEKTLQSPLEYTIVPHAYDG
                     IKHFYMRPDLQLLQIFRCDTPMYGLAVRPGFEYTDDMLDKTVIVSHPSPINLIKYFTRK
                     DVTFDLVNSTSAAAKRVKDGLSDIALTNELARQKYGLHFVKTFKSIPMSWSLFGKGEIH
                     DEN"
     source          1..4342
                     /db_xref="taxon:679622"
                     /mol_type="genomic DNA"
                     /organism="Bacillus sp. CS93"
                     /strain="CS93"
     misc_feature    <1..>4342
                     /note="bacilysin biosynthetic gene cluster"
     subregion       1..4342
                     /aStool="mibig"
                     /contig_edge="True"
                     /label="Other"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..4342
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            491..1201
                     /gene="bacB"
     CDS             491..1201
                     /codon_start=1
                     /gene="bacB"
                     /note="anticapsin synthesis"
                     /product="BacB"
                     /protein_id="ACX35427.1"
                     /transl_table=11
                     /translation="MKTEQDLQELYFPTPKLIEWDNGVRQYSSVRGDTEVLLSYVPPHT
                     NVEPHQHKEVQIGLVVSGELSMTVGDVTRKMTALESAYIAPPNVPHGARNETDQEVIAI
                     DIKRLKAGETYTSPEDYFLNIFKTRDLLPGMEVTFFVEDWVEIMLANIPGNGGEMPFHK
                     HRNEQIGICIGGGYDMTIEGCKVDMTFGTAYFCDPREDHGAINRFEKDSKSVNIFFPPR
                     YNRAKAKKLEAKES"
     gene            1198..1959
                     /gene="bacC"
     CDS             1198..1959
                     /NRPS_PKS="Domain: PKS_KR (6-116). E-value: 2.3e-11. Score:
                     35.8. Matches aSDomain: nrpspksdomains_bacC_PKS_KR.1"
                     /codon_start=1
                     /gene="bacC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short_C2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001:short-chain dehydrogenase/reductase SDR (Score:
                     238.4; E-value: 1.4e-72)"
                     /gene_kind="biosynthetic-additional"
                     /note="anticapsin synthesis"
                     /product="BacC"
                     /protein_id="ACX35428.1"
                     /sec_met_domain="adh_short (E-value: 6.2e-38, bitscore:
                     119.0, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short_C2 (E-value: 2e-32, bitscore:
                     101.5, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNLTDKTVLITGGASGIGYAAVQAFLNQEANVVVADIDEAQGEAM
                     IRKENNDRLHFVRTDITDEPACQNAIRSAVDKFGGLDVLINNAGIEIVAPIHEMELSDW
                     DKVLNVNLTGMFLMSKHALKYVLKSGKGNIINTCSVGGVVAWPDIPAYNASKGGVLQLT
                     RSMAVDYAKHNIRVNCVCPGIIDTPLNEKSFLENNEGTLEEIKKEKAKVNPLLRLGKPE
                     EIANVMLFLASDLSSYMTGSAITADGGYTAQ"
     CDS_motif       1213..1266
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_bacC_0001"
                     /evalue="3.60E-05"
                     /label="PKSI-ER_m5"
                     /locus_tag="bacC"
                     /protein_end="23"
                     /protein_start="5"
                     /score="16.4"
                     /tool="antismash"
                     /translation="KTVLITGGASGIGYAAVQ"
     aSDomain        1216..1545
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_bacC_PKS_KR.1"
                     /evalue="2.30E-11"
                     /label="bacC_PKS_KR.1"
                     /locus_tag="bacC"
                     /protein_end="116"
                     /protein_start="6"
                     /score="35.8"
                     /tool="antismash"
                     /translation="TVLITGGASGIGYAAVQAFLNQEANVVVADIDEAQGEAMIRKENN
                     DRLHFVRTDITDEPACQNAIRSAVDKFGGLDVLINNAGIEIVAPIHEMELSDWDKVLNV
                     NLTGMF"
     gene            1977..3395
                     /gene="bacD"
     CDS             1977..3395
                     /codon_start=1
                     /gene="bacD"
                     /gene_functions="biosynthetic (rule-based-clusters) other:
                     bacilysin"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1063:argininosuccinate lyase/adenylosuccinate lyase
                     (Score: 67.4; E-value: 2.1e-20)"
                     /gene_kind="biosynthetic"
                     /note="amino acid ligase"
                     /product="BacD"
                     /protein_id="ACX35429.1"
                     /sec_met_domain="bacilysin (E-value: 0.0, bitscore: 1034.9,
                     seeds: 8, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MERKTVLVIADLGGCPPHMFYKSAAEKYNLVSFIPRPFAITASHA
                     ALIEKYSIAVIKDKDYFKSLADFEHPDSIYWAHEDHDKPEEEVVEEIVKVADMFAVDAI
                     TTNNELFIAPMAKACERLGLRGAGVQAAENARDKNKMRAAFNRAGVKSIKNKRVTTLED
                     FRAALQEIGTPLILKPTYLASSIGVTLIKERETAEAEFNRVNEYLKSINVPKAVTFEAP
                     FIAEEFLQGEYDDWYETSGYSDYISIEGIMADGEYSLVAIHDKTPQIGFTETSHITPSI
                     LDDDAKRKIVEAAKKANEGLGLENCATHTEIKLMKNREAGLIESAARFAGWNMIPNIKK
                     VFGVDMAQLLLDVLCFGKEADLPNGLLEQEPCYVADCHLYPQHFKENGQLPETAVDFVI
                     ESIDIPDGVLKGDTEIVSFSAAEAGTSVDLRLFEAFNSIAAFELKGSNSGDVAESIKQI
                     QQQAKLTAKYALPV"
     gene            3392..>4342
                     /gene="bacE"
     CDS             3392..>4342
                     /codon_start=1
                     /gene="bacE"
                     /gene_functions="transport (smcogs) SMCOG1020:major
                     facilitator transporter (Score: 122.8; E-value: 2.8e-37)"
                     /gene_kind="transport"
                     /note="anticapsin excretion"
                     /product="BacE"
                     /protein_id="ACX35430.1"
                     /transl_table=11
                     /translation="MKQLKPNSKYLLFGQALSFMGDYCVLPALLILSTYYHDYWVTSGV
                     IAVRSIPMVFQPFLGVLVDRLDRVKIMLWTDVIRGVIFLGLTFLPKGEYPLLFLALLFI
                     SYGSGVFFNPARLAVMSSLEADIKNINTLFAKATTISIIVGAAAGGLFLLGGSVELAVA
                     FNGVTYLVSAFFISRIKLQYVPIQSENVREAFQSFKEGLKEIKTNAFVLNAMFTMITMA
                     LLWGVVYSYFPIVSRFLGDGEIGNFILTFCIGFGGFIGAALVSKWGFNNNKGLMYFTVL
                     SIVSLALFLFTPIFAVSVIAAILFFIAMEYGEVLAK"
ORIGIN
        1 aacttcatca ccaatttcac ccttctccaa ggggtgaaca gcaaattatc actgcatgat
       61 acatttgaat cgtgcattga aaaaacgctt caaagtccgc tggaatacac aattgtgcct
      121 cacgcgtatg acggcattaa gcacttctac atgagaccgg atctgcagct tctgcaaatt
      181 ttcaggtgcg acaccccgat gtacggcctg gctgtccggc ccggttttga atatacagat
      241 gacatgcttg ataagactgt catcgtatcc cacccgtctc ctatcaattt gattaagtat
      301 tttacccgta aagatgtcac gtttgatctg gtgaactcaa cgagcgcagc cgcgaaaaga
      361 gtgaaggacg gtttgtccga catcgcgctg acaaatgagc tggccagaca aaagtacggc
      421 cttcactttg tcaaaacatt taaaagcatt cccatgagct ggtcattatt cggaaaagga
      481 gagattcatg atgaaaactg aacaagattt gcaggaactt tatttcccga cgcctaaatt
      541 aatagaatgg gataacggag taagacaata ttcgtccgtc agaggcgata cggaagttct
      601 gctttcttac gttccgccgc atacaaacgt ggaaccgcat cagcataagg aagtccaaat
      661 cggccttgtc gtttccggcg agctgtccat gactgtcgga gatgtcacaa gaaagatgac
      721 agcgctggaa tcagcctata tcgcgcctcc taacgtgcct cacggcgcaa gaaatgagac
      781 ggatcaagaa gtgatcgcca ttgatattaa acgcctgaaa gccggtgaaa cctatacaag
      841 ccctgaggat tattttttga atatttttaa aacaagagac ttattgcctg ggatggaagt
      901 cacgttcttc gtggaagact gggtggaaat catgcttgcc aacattccgg gcaacggagg
      961 agaaatgccg ttccataaac accgcaatga acagatcggc atctgcatcg gcggcggcta
     1021 tgatatgacc attgaaggct gcaaagtgga tatgacattc ggtacagctt atttctgcga
     1081 tccgagagaa gatcacggcg ccatcaacag atttgaaaaa gattcaaaat cagtcaatat
     1141 ctttttccct ccgcgctata acagagcaaa agcgaaaaaa ttagaggcga aagagtcatg
     1201 aacctgacgg ataaaaccgt cctcatcaca gggggcgcat caggcatcgg ttatgccgcg
     1261 gttcaggcgt ttctgaatca agaggcaaat gtggtcgtcg cggatattga tgaggcgcag
     1321 ggagaagcga tgatccggaa agaaaacaat gacaggctgc attttgtccg gacggatatt
     1381 acagatgaac cggcatgtca aaacgccatc cggtcagcgg ttgataagtt cggcggattg
     1441 gatgtgttaa tcaataacgc cggcatcgaa attgtcgccc cgattcatga gatggagctg
     1501 agcgattggg acaaagtgct caacgtcaat ctgacgggca tgtttcttat gagcaagcac
     1561 gcgctcaagt atgtgctcaa aagcggcaag ggcaatatca ttaacacgtg ctccgtcgga
     1621 ggagtcgtcg catggcccga cattcccgca tataacgctt ctaaaggcgg cgtattgcag
     1681 ctgacgcgtt cgatggccgt cgattacgcc aaacacaaca tccgcgtcaa ttgcgtgtgc
     1741 ccgggaatca ttgacacacc tttgaatgaa aagtcgttcc ttgaaaacaa cgaaggcacg
     1801 cttgaagaaa tcaaaaaaga aaaagcgaaa gtcaatccgc tgctgagact cggcaagcct
     1861 gaagaaatag cgaacgtcat gctgtttctc gcttcggatt tatcaagcta tatgaccgga
     1921 agcgcgatca ccgcagacgg tggatacacc gcacaataga gaaggagtgt ttacatatgg
     1981 agagaaaaac agtattggtt atcgctgacc ttgggggatg cccgccgcat atgttttaca
     2041 aaagcgcagc cgaaaaatac aacctcgtca gcttcattcc gagacctttt gcaattacag
     2101 cctctcatgc ggcattaatt gaaaaatact cgattgcggt cattaaagat aaagactatt
     2161 ttaagagtct ggctgatttt gagcatcccg attcgattta ctgggctcat gaagatcatg
     2221 acaaacctga ggaagaagtc gtcgaagaaa tcgtcaaggt ggctgacatg ttcgcggttg
     2281 acgccattac gaccaacaat gaactgttta tcgctccgat ggcaaaagcg tgtgaacgtc
     2341 tcggcctgcg gggagcgggc gtacaggcgg ctgaaaatgc cagagataaa aacaaaatga
     2401 gagccgcctt caaccgggcc ggcgtcaagt ctatcaaaaa caaacgggtg acgacgctgg
     2461 aagatttccg cgccgcgctt caggaaatcg gaacgccgct tattctgaag cctacatatc
     2521 tggcgagctc catcggcgtg acgctcatca aagagaggga aacggcagaa gcagaattta
     2581 acagagtcaa tgaatacctg aagtcgatta acgtaccgaa agcggtcacg tttgaagcgc
     2641 cttttatcgc ggaagaattt ttgcagggcg agtatgacga ctggtacgaa acaagcggtt
     2701 attccgacta tatcagcata gaaggcatca tggccgacgg ggaatactcc cttgtcgcga
     2761 ttcatgataa aacaccgcaa atcggattca cggagacatc gcatattacg ccgtccatcc
     2821 tggatgatga cgcgaagcgg aaaatcgtcg aagctgccaa aaaggcgaat gaaggactcg
     2881 gcctcgaaaa ctgcgcgaca catacagaaa taaaattaat gaaaaaccgg gaagccggat
     2941 tgattgaatc agcggcacga tttgcgggct ggaacatgat tccgaatatt aaaaaggtct
     3001 tcggcgtcga tatggcgcag ctgttattgg atgttctctg tttcggaaaa gaagccgatc
     3061 tgccgaatgg attattggag caggagccgt gctatgtcgc cgactgccac ttgtatcctc
     3121 agcatttcaa agagaacggc cagctgcctg agaccgctgt cgatttcgtc attgaaagca
     3181 ttgacattcc tgacggcgtc ttaaagggag ataccgaaat cgtttctttc tcggcggccg
     3241 aggcgggtac gtccgtggat ctgcggctgt tcgaagcgtt caacagcatt gcggcgttcg
     3301 agctgaaagg aagcaattcg ggtgacgtgg ccgaatcaat caaacaaatt cagcagcagg
     3361 cgaagctgac tgcaaagtat gcgttaccgg tatgaaacag ctgaaaccga actctaagta
     3421 tttgcttttc gggcaggcgc tttcgtttat gggagactat tgcgttctcc cggccctgct
     3481 cattttgtct acatactatc acgactactg ggtgacgtcc ggcgtcatcg cggtccgcag
     3541 tattccgatg gtgtttcagc cgtttttagg cgtgctcgtg gaccggctcg acagggtgaa
     3601 aatcatgctg tggacggatg tgatcagagg tgtcatcttt ttggggctga catttctccc
     3661 gaaaggcgaa tatccgctgc tttttctggc gctgctgttt atttcttacg gaagcggcgt
     3721 gttctttaac ccggcacgtc tcgcggtcat gtcgtcttta gaagcggata ttaaaaatat
     3781 caacactttg tttgcaaaag ccacgacgat ttcgattatt gtcggcgctg cagccggcgg
     3841 actttttctg ttaggcggtt ctgtggagct tgccgtcgcg tttaacgggg tgacctatct
     3901 tgtctccgcg tttttcatca gccgcattaa gcttcaatac gtgccgattc agtcagaaaa
     3961 cgtaagagaa gcttttcagt cgtttaaaga aggcctgaag gaaatcaaaa caaatgcttt
     4021 tgtgttaaac gccatgttca cgatgattac gatggcgctt ttgtggggtg tcgtctacag
     4081 ctatttcccg attgtcagcc gctttttagg cgacggggaa atcggcaatt tcatcctgac
     4141 gttctgtatc ggcttcggcg gctttatcgg cgccgctctt gtcagcaaat ggggctttaa
     4201 caacaataaa gggctgatgt attttacagt gttatcgatc gtctcgcttg cgctgttttt
     4261 gtttaccccg attttcgccg tgtcagtcat tgccgccatt cttttcttta tcgcaatgga
     4321 atacggtgag gtgctggcga ag
//
