LOCUS       BGC0000890             11090 bp    DNA     linear   BCT 09-JUL-2014
DEFINITION  Pseudoalteromonas phenolica O-BC30 bmp gene cluster, complete
            sequence.
ACCESSION   BGC0000890
VERSION     BGC0000890
KEYWORDS    .
SOURCE      Pseudoalteromonas phenolica O-BC30
  ORGANISM  Pseudoalteromonas phenolica O-BC30
            Bacteria; Pseudomonadota; Gammaproteobacteria; Alteromonadales;
            Pseudoalteromonadaceae; Pseudoalteromonas.
COMMENT     ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2024-10-17 14:22:56
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 11090
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     gene            complement(1..1173)
                     /gene="bmp10"
     CDS             complement(1..1173)
                     /codon_start=1
                     /gene="bmp10"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pyr_redox_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1175:pyridine nucleotide-disulfide oxidoreductase
                     (Score: 134.6; E-value: 6.1e-41)"
                     /gene_kind="biosynthetic-additional"
                     /product="Bmp10"
                     /protein_id="AHA34031.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 3.7e-69, bitscore:
                     222.5, seeds: 64, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MARCIIIGGGHAGAQACISLRKEGWTDEIVLISDEHSLPYHRPPL
                     SKNYLLGQVDEKGLLIRPEQAYQKDNITLKLRTRVARIEPALQSVVLQNGERLSYEHLL
                     LCTGSRARRITCSGNELSNIFYIKTLKDIEQLAARLSTNKQRIVMVGGGYIGLETAAVL
                     NKMGHQITLLEQAPRLLARVTAPFISEFYQQLHQQHGVEVHTNTQVLAFTGEQNVTGVR
                     CKALTQSDTAEQYFNADLVIVGIGAEINTELAEQAGLLVEQNGIVVNELCQTTDKNIYA
                     AGDCTIGIEHHSGIATRIESVPNALAQAKTAAATICGKHQAVTSVPWFWSDQYDCKIQI
                     AGLNHGYDNTVLRQHSSSSFSLWYLKQGRIIAADCINTAKDFMAAKKDHR"
     source          1..11090
                     /country="Japan: Ogasawara Island, Tokyo"
                     /culture_collection="JCM:21460"
                     /db_xref="taxon:1315281"
                     /isolation_source="seawater"
                     /mol_type="genomic DNA"
                     /organism="Pseudoalteromonas phenolica O-BC30"
                     /strain="O-BC30"
                     /type_material="type strain of Pseudoalteromonas phenolica"
     misc_feature    1..11090
                     /note="bmp gene cluster"
     subregion       1..11090
                     /aStool="mibig"
                     /contig_edge="True"
                     /label="other"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..11090
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            complement(1220..1540)
                     /gene="bmp9"
     CDS             complement(1220..1540)
                     /codon_start=1
                     /gene="bmp9"
                     /product="Bmp9"
                     /protein_id="AHA34032.1"
                     /transl_table=11
                     /translation="MPEITFIDFSGNETTIDVPTGTSLMQAAMENMIDGIEAECGGSGS
                     CATCHCYLTDDWQGKVNEPNSSEADMLEMVSEPTELSRLSCQVLVSDEHHGLQVRLPES
                     QY"
     gene            complement(1549..2139)
                     /gene="bmp8"
     CDS             complement(1549..2139)
                     /codon_start=1
                     /gene="bmp8"
                     /product="Bmp8"
                     /protein_id="AHA34033.1"
                     /transl_table=11
                     /translation="MQQTKNHLVSPVSRADIPELEPILQGIENNLGFIPNGILTMAKNP
                     MLAKAFGQLFGCLSQFEHISTELKWAIATISSNAAGCQYCQGHFSHIASRVNVNRAKVL
                     AAFEFETSILYDDAERAALRFAFAASTSPAHLEKHHYDNLGKHFSEAAVIEISAVIAIC
                     GFLNRWNTTMDSQLEADPAAILAEINQLSRFSV"
     gene            2372..3445
                     /gene="bmp1"
     CDS             2372..3445
                     /NRPS_PKS="Domain: PP-binding (11-74). E-value: 1.3e-08.
                     Score: 27.2. Matches aSDomain:
                     nrpspksdomains_bmp1_PP-binding.1"
                     /codon_start=1
                     /gene="bmp1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1036:alpha/beta hydrolase fold protein (Score: 71.8;
                     E-value: 1.3e-21)"
                     /gene_kind="biosynthetic-additional"
                     /product="Bmp1"
                     /protein_id="AHA34034.1"
                     /sec_met_domain="PP-binding (E-value: 2.3e-09, bitscore:
                     27.2, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Abhydrolase_6 (E-value: 1.1e-14, bitscore:
                     45.0, seeds: 455, tool: rule-based-clusters)"
                     /sec_met_domain="PF00561 (E-value: 5.5e-13, bitscore: 38.5,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIEELIDFINNELLEGAAPDLDQHTPLLELGILNSLSMVRLLAHV
                     DQRYGAKIPEHDITPVHFENIETLAALINTLSAKDEMQAEQSVSELERLVKLQESYGIQ
                     SELVDAGEGFKQHVLRVKGDGPLWILLPALGNPSTSWSSTLRSVQGRHNAVALDLAGFG
                     LSQSANEAPSYVDHVEFTLQYLATLEEQEWVLVANSAGSMIATEIARRFPEKVKALVCS
                     GFGLVEDVENWWGDLKKMSSNPEQFLAAAYYHPPKMTPALESLLDDVLSRPAYHSFLDD
                     YARQSIAKTFDGVMVPTLFVAGNHDQIIPPEWSEKAAELLDKASVTRLSRCGHFSASER
                     PEEFVWVVEDFLNREAM"
     aSDomain        2405..2593
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_bmp1_PP-binding.1"
                     /evalue="1.30E-08"
                     /label="bmp1_PP-binding.1"
                     /locus_tag="bmp1"
                     /protein_end="74"
                     /protein_start="11"
                     /score="27.2"
                     /tool="antismash"
                     /translation="ELLEGAAPDLDQHTPLLELGILNSLSMVRLLAHVDQRYGAKIPEH
                     DITPVHFENIETLAALIN"
     gene            3445..4665
                     /gene="bmp2"
     CDS             3445..4665
                     /codon_start=1
                     /gene="bmp2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pyr_redox_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Trp_halogenase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1119:halogenase (Score: 270.3; E-value: 5.9e-82)"
                     /gene_kind="biosynthetic-additional"
                     /product="Bmp2"
                     /protein_id="AHA34035.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 1.2e-09, bitscore:
                     27.2, seeds: 64, tool: rule-based-clusters)"
                     /sec_met_domain="Trp_halogenase (E-value: 5.9e-17,
                     bitscore: 50.9, seeds: 23, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDQFKSYDVVIIGSGPAGSLCGIECRKKGLSVLCIEKDEFPRFHI
                     GESLTGNAGQIIRDLGLADEMNAAGFPDKPGVNVIGSLSKNEFFIPILAPTWQVRRSDF
                     DNMLKRRALEHGVEYQQGLVKDVIKHEEKVVGAIYKADGVEHQVRSKVLVDASGQNTFL
                     SRKGIAGKREIEFFSQQIASFAHYKNVERDLPPFSTNTTILYSKQYHWSWIIPISPDTD
                     SLGIVIPKDLYYKECKNPDDAIEWGMEHISPEIRRRFKNAERVGESQSMADFSYRIEPF
                     VGDGWLCIGDAHRFLDPIFSYGVSFAMKEGIKAADAIKRAIDGNDWKTPFYEYRDWSNG
                     GQQIAADLIRYFWIYPIFFGYQMQNPDLRDEVIRLLGGCCFDCEGWKAPTIFRNAIEEY
                     DRKQMAG"
     gene            4675..5817
                     /gene="bmp3"
     CDS             4675..5817
                     /codon_start=1
                     /gene="bmp3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_M"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1006:acyl-CoA dehydrogenase (Score: 313.2; E-value:
                     4.2e-95)"
                     /gene_kind="biosynthetic-additional"
                     /product="Bmp3"
                     /protein_id="AHA34036.1"
                     /sec_met_domain="Acyl-CoA_dh_N (E-value: 2.4e-17, bitscore:
                     53.1, seeds: 790, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_M (E-value: 3.5e-23, bitscore:
                     71.2, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_1 (E-value: 4e-36, bitscore:
                     113.9, seeds: 49, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNFAWNEDQLALHEHALSFARDLDFSKISAGEFPMEAWQSLGEFG
                     YYGLPVDSRYSPCGSGFDILTTVKILEGLGQGCADTGILFAGAAHTFACVMPIYEHGSE
                     TLKHELLPALTSGEKIAANAISEAEAGSDIGKLTTKATREGDYYIINGAKSYVTNGSVA
                     DYFVLYATTNPKFGYLGQTAFVVPADLPGIEVGKSYKKLGLDSAQLNQVFFDEVKVHKD
                     YVLGSEGQGARIFAASMDWERCCLFALFIGVMQNDLERCIEFANTRKQGDKTISKFQAV
                     SHRIADMEVRLQSARLLLYFAAWQKSQDVDNTKAVASSKLAISEAFVQSGIDAIRIHGA
                     AGYLQESGIDSSIRDALGSVLFSGTSDIQRELICNRLGLL"
     gene            5814..7361
                     /gene="bmp4"
     CDS             5814..7361
                     /NRPS_PKS="Domain: AMP-binding (16-410). E-value: 5.2e-94.
                     Score: 307.0. Matches aSDomain:
                     nrpspksdomains_bmp4_AMP-binding.1"
                     /codon_start=1
                     /gene="bmp4"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002:AMP-dependent synthetase and ligase (Score:
                     300.3; E-value: 3.5e-91)"
                     /gene_kind="biosynthetic-additional"
                     /product="Bmp4"
                     /protein_id="AHA34037.1"
                     /sec_met_domain="AMP-binding (E-value: 9.1e-105, bitscore:
                     339.9, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLLNQVLTQGLVRAGEQALAVSDSTESYSYGELQLRATQIAVLL
                     QKIGVKAGDRVGLWCDKSCRALAGMLAVSKLNAVYVPIDPMNPKVRVDIIRQDCDMKVL
                     ITNKAKFSQYQRGDNALCDFVVLDEDALISSVLHVHTWQHISAQQPSQLNLPKEGKSTD
                     LAYMLYTSGSTGVPKGVKISHENALAFIEWSTNKLNATVQDRFANHAPWHFDLSVLDIY
                     VALLSGASVHLVSEIDSYVAQKLVSFVAQKQISIWYSVPTALVMMLEAEADFAERCQQL
                     RAIIFAGEAFAIKPLKQLREAFPDAELYNFYGPTETNVCSYYKVPRVVPDKLPIGYPAS
                     NALLSICDEQGKALPVGEQGFVTVIGPTVFSGYWGRAELQSEAYNTGDIGYFNELGELM
                     YVGRQDHMVKVNGYRVHLGEVERAIYQHPAVKECLVELSSQKTLHAHIAVEGDAPSLLT
                     LKIHCAKRLPKYMLPEQVIVHDALPRNRNGKLSRQCLSGLSAQSGSENNTQITMSEA"
     aSDomain        5862..7043
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_bmp4_AMP-binding.1"
                     /evalue="5.20E-94"
                     /label="bmp4_AMP-binding.1"
                     /locus_tag="bmp4"
                     /protein_end="410"
                     /protein_start="16"
                     /score="307.0"
                     /tool="antismash"
                     /translation="EQALAVSDSTESYSYGELQLRATQIAVLLQKIGVKAGDRVGLWCD
                     KSCRALAGMLAVSKLNAVYVPIDPMNPKVRVDIIRQDCDMKVLITNKAKFSQYQRGDNA
                     LCDFVVLDEDALISSVLHVHTWQHISAQQPSQLNLPKEGKSTDLAYMLYTSGSTGVPKG
                     VKISHENALAFIEWSTNKLNATVQDRFANHAPWHFDLSVLDIYVALLSGASVHLVSEID
                     SYVAQKLVSFVAQKQISIWYSVPTALVMMLEAEADFAERCQQLRAIIFAGEAFAIKPLK
                     QLREAFPDAELYNFYGPTETNVCSYYKVPRVVPDKLPIGYPASNALLSICDEQGKALPV
                     GEQGFVTVIGPTVFSGYWGRAELQSEAYNTGDIGYFNELGELMYVGRQDHMVKV"
     CDS_motif       6306..6365
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_bmp4_0001"
                     /evalue="2.10E-09"
                     /label="NRPS-A_a3"
                     /locus_tag="bmp4"
                     /protein_end="184"
                     /protein_start="164"
                     /score="29.4"
                     /tool="antismash"
                     /translation="AYMLYTSGSTGVPKGVKISH"
     CDS_motif       6903..6962
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_bmp4_0002"
                     /evalue="1.30E-03"
                     /label="NRPS-A_a6"
                     /locus_tag="bmp4"
                     /protein_end="383"
                     /protein_start="363"
                     /score="11.3"
                     /tool="antismash"
                     /translation="VIGPTVFSGYWGRAELQSEA"
     CDS_motif       6981..7076
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_bmp4_0003"
                     /evalue="1.40E-09"
                     /label="NRPS-A_a8"
                     /locus_tag="bmp4"
                     /protein_end="421"
                     /protein_start="389"
                     /score="30.1"
                     /tool="antismash"
                     /translation="GYFNELGELMYVGRQDHMVKVNGYRVHLGEVE"
     gene            7408..8955
                     /gene="bmp5"
     CDS             7408..8955
                     /codon_start=1
                     /gene="bmp5"
                     /gene_functions="biosynthetic (rule-based-clusters) PBDE:
                     FMO-like"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pyr_redox_2"
                     /gene_functions="other (smcogs) SMCOG1092:hypothetical
                     protein (Score: 281.8; E-value: 2.1e-85)"
                     /gene_kind="biosynthetic"
                     /product="Bmp5"
                     /protein_id="AHA34038.1"
                     /sec_met_domain="FMO-like (E-value: 6.4e-87, bitscore:
                     281.4, seeds: 5, tool: rule-based-clusters)"
                     /sec_met_domain="Pyr_redox_2 (E-value: 5.6e-12, bitscore:
                     34.8, seeds: 64, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRKKIAVIGAGLSGIAAIKQLTDGGHEVVCFEKAESFGGVFADKK
                     IYEDLHLTISNYFMAYSDYVPSQQKLKFWSKKEYVNYLGEYLARFELGQYIHYDHEVRK
                     VEKQAGKWQVTTKHGMAEQTDTFDMVAVCSGHFQKPKMPELAGLDMFEGEIEHSNDYRD
                     KHKYAGKRVLCVGLGESSADITSEISEVASKCILSLRRYPAVAPRYMAFQEDPYFTIDT
                     SWLTSRIVNKLPHRYHGGITKGIFTKYVNSRNDHVRIRGEWLKKSGPSHHQAVTKNERL
                     FRPIADGKVVPNIGGIVRFEKNAVVFQDGTREEIDAVVFCTGYQLSFPFLDVSISNMRD
                     LYKQMFIPEMGDSLSFIGFVRPQQGGIPVIAEMQCRYLSQLASGEKSLPPLSEMVDIIK
                     YDTEHWQTEYKITPHVASLVNYCHYMDSMAKLVGCMPEIPSLFKDPLLRVKLLHNPQFA
                     AQYRLDGPNKMTHTARSFLLGFPNISSWPRIIHFELALITQKLLSRLRLDGLRELSK"
     gene            8979..9608
                     /gene="bmp6"
     CDS             8979..9608
                     /codon_start=1
                     /gene="bmp6"
                     /gene_functions="biosynthetic (rule-based-clusters) PBDE:
                     DUF98"
                     /gene_kind="biosynthetic"
                     /product="Bmp6"
                     /protein_id="AHA34039.1"
                     /sec_met_domain="DUF98 (E-value: 2.8e-17, bitscore: 52.5,
                     seeds: 20, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIRLTDECLCNLDDALLPHRFNLSRLSHLQKIILGTDGTVTQLIE
                     SIVDEKLVVNKLFEADVDSDTIATTSSEQQAEIHCQRRVILLQGETSGLPYLYADSLVY
                     HGNMNVNFSRALTHSQIPIGKAWEKFQVETYKTLLSWGFEYANELAPHFAISSHDLVLY
                     RTYLVFSQGKKIFRITEKFPFVWYLQSEQFTASAATTATPTLVSGI"
     gene            9612..11090
                     /gene="bmp7"
     CDS             9612..11090
                     /codon_start=1
                     /gene="bmp7"
                     /gene_functions="biosynthetic (rule-based-clusters) PBDE:
                     p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1034:cytochrome P450 (Score: 186.1; E-value: 1.9e-56)"
                     /gene_kind="biosynthetic"
                     /product="Bmp7"
                     /protein_id="AHA34040.1"
                     /sec_met_domain="p450 (E-value: 1.2e-71, bitscore: 231.1,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKNTDSVVAQPQINLDINKLTPSIPHIPFNSEDGQLFFWGEDYHL
                     IIESLSKKYGDIYSVETAKGNLVVLNGYQYIKEALVTQNDVFNVRADYKILQVAPQKHF
                     LELEAGELWQVHRKVFAHAMREYFATRWDSIEQWMTTEIEDIAAVWQADEGKPFDPNRE
                     VSIKLASFLHKVMFDDRFGEFEKGLFDEKSLSWLPNGFINSTRYELMPTELQEKYYAQY
                     GDAIEKFSGNLNALDQYVSFNVDRRKADYKPGEYRDLCDYLFAANDSVDEQTKAALNID
                     EKEIVIGSLTQVAGAGGGVGAFAMRWALLYLAAFPKQQQRVQKELDEVVGKNAVPLNKH
                     KADLPYTQAFISEVLRHCSITSMPAANYATSKDTLLDGYFLAEGTPLVINNYSITRDET
                     LWENPHEFMPERFLDEDGELSKKQLGKAFPFGLGQRRCLGEHFGKYIIHTLFAQLAHKF
                     EFSLPEGKQANLKAISGVFLVPEEVEIVAKSRNA"
ORIGIN
        1 ttatctatga tctttttttg cagccataaa atcttttgcc gtgttaatac agtcagctgc
       61 gatgatgcga ccttgtttta aataccacag tgaaaaactg cttgatgaat gttgacgtaa
      121 tacggtgttg tcatagccat gatttaagcc cgcgatctga attttacagt cgtattgatc
      181 agaccaaaac caaggtactg acgtcaccgc ttggtgttta ccacaaatag tcgcggcagc
      241 tgtttttgct tgtgctaacg catttggcac agattcaata cgtgttgcaa tgccactgtg
      301 atgttctata cctatggtgc aatcgcccgc agcataaata tttttatctg tggtttggca
      361 tagttcgttg actacaatgc cattttgttc cacgagtaat cctgcttgtt cagcaagttc
      421 agtgttaatt tcggcgccga taccaacaat gactaaatcg gcattaaaat actgctcagc
      481 tgtgtctgat tgggttaatg ctttacagcg cactccagtc acgttttgct caccggtgaa
      541 tgctaaaact tgtgtgttgg tatgtacttc tacgccatgt tgttggtgga gctgctgata
      601 aaactcagaa ataaaggggg cggtaacgcg agcaagcaat ctcggcgctt gctctagtag
      661 agtgatttga tggcccattt tattgagtac agctgcagtt tctaaaccaa tgtagccccc
      721 tccaaccatc acaatacgtt gtttatttgt gcttagccgt gcggctagct gctcaatgtc
      781 ttttagggtt ttaatataaa aaatattgct taattcattg cctgaacaag taattctacg
      841 cgctcgactg ccagtacaga gtagcaagtg ttcatagctt agacgctcac cattttgcag
      901 taccacactt tgtaaagcgg gctctattct tgctactcga gtgcgtagtt ttagggtgat
      961 gttatctttt tggtaggctt gctcaggtct tattaataag cctttttcat ctacctgacc
     1021 cagtaaataa tttttagaaa gcggagggcg atggtagggc aagctgtgtt catcactgat
     1081 caaaacgatt tcgtcagtcc agccttcttt ccttaagcta atacaagctt gcgcgccagc
     1141 atgtccgccg ccaatgatga tgcatcttgc catgatgtta tcccttaatt ttgtgacaaa
     1201 aattcagatt gctcagactc tagtattgag attcaggtaa tctcacttgt aagccatggt
     1261 gttcatcaga cactaatacc tggcaactta gacgacttaa ttcagtcggc tctgagacca
     1321 tttctagcat atctgcttca ctgctatttg gttcgtttac tttgccttgc cagtcgtctg
     1381 ttaagtaaca atgacaggtt gcgcaactgc cagaaccacc acactcagct tcaataccgt
     1441 cgatcatgtt ttccatcgcg gcttgcatta aagatgtgcc tgtcggcaca tctatggtgg
     1501 tttcattgcc gctaaaatca ataaaagtaa tttcaggcat aacaactcct acacagaaaa
     1561 acgactaagt tgattaattt cagccaagat agccgccgga tcggcctcta gttgactgtc
     1621 catggtggta ttccagcgat ttaaaaagcc acaaatggcg atgacagcag aaatctcaat
     1681 gactgccgct tcactaaagt gcttacctaa gttgtcataa tggtgttttt ctagatgagc
     1741 aggcgaggtg cttgctgcaa atgcaaagcg aagtgctgcg cgttcagcat cgtcatataa
     1801 aatgctggtt tcaaattcaa atgcggccaa gactttggca cgattcacat tcacgcgact
     1861 ggcgatatgc gagaagtgcc cttgacaata ttggcacccc gcagcattgg agctgatggt
     1921 ggcaattgcc cattttagtt ctgtgcttat gtgctcaaat tggcttaagc atccaaacaa
     1981 ctgcccaaat gctttagcta gcattgggtt tttagccatg gtcaaaatac catttggtat
     2041 aaaccctaga ttgttctcaa taccctgcaa aatgggctct aactcaggaa tatcagcgcg
     2101 actaaccggg ctgactaagt ggtttttagt ttgctgcatt gcagtcttcc taactgtgat
     2161 agcgagccat caaggtatag ctaagcgtag aacattccta tagtcccaac ggatcaaggc
     2221 catagtccac aggggctata tggctataac aacattccct gtaaggtttt taatagattc
     2281 aaactgaacc cgaacattcg cggcatcgct tattattcaa agcaaataag tcgtggctga
     2341 aggatttgca aagactagac aggaaactag catgattgaa gaactaatcg actttataaa
     2401 taacgaatta cttgaaggtg cggcgcctga tttagatcag cacacaccgc ttttagagct
     2461 aggtatttta aactcactat cgatggtgag attacttgcg catgtagatc agcgttacgg
     2521 tgcgaagatc cctgagcacg atattacccc tgtgcatttt gagaatatag aaacacttgc
     2581 agcgcttatt aacaccttaa gcgcaaaaga tgaaatgcag gctgagcaat cggtatctga
     2641 gctagagcga ctggtcaagt tacaggaaag ctatggcatt cagagtgagc tggtggatgc
     2701 aggtgaaggc tttaagcagc atgtattgcg agttaaaggc gatggtcctt tatggatttt
     2761 actccctgca cttggcaacc cttcaacatc ttggtcttcc acgttgcgat cagtacaagg
     2821 tcgtcacaat gctgtagcgc ttgatttagc gggttttggt ttgagccaaa gtgccaatga
     2881 agcgccatct tatgttgatc atgttgagtt tacattgcaa taccttgcaa cgcttgaaga
     2941 gcaagagtgg gtgctggtgg cgaactccgc aggcagcatg attgcgaccg agattgcacg
     3001 tcgcttccct gagaaagtaa aagccctggt ttgtagtggc tttggtttag ttgaagacgt
     3061 cgaaaattgg tggggcgatt taaagaaaat gtcgtcaaac ccagagcaat tcttggcagc
     3121 cgcttattac cacccaccaa agatgacgcc agccttagag tcattactag atgatgttct
     3181 gtctcgccct gcctatcaca gcttcttaga tgactatgcg cgtcagtcta tcgcgaaaac
     3241 ctttgatggg gtgatggtac ctactttatt tgttgcaggt aatcacgacc aaattattcc
     3301 tccagaatgg agtgaaaaag ccgctgagct tcttgataaa gccagtgtta ctcgcctttc
     3361 tcgctgtggt cacttctcgg cttctgaacg cccagaagag tttgtttggg tagtggaaga
     3421 tttcttaaat agggaagcaa tgtaatggat cagtttaaaa gttacgacgt tgttattatt
     3481 ggcagtggcc cagcaggcag cttatgcggt atagagtgtc gcaaaaaagg cttatcggtt
     3541 ttatgtatcg aaaaagatga gtttccgcgc tttcacattg gtgagtcact gactggtaac
     3601 gctgggcaga tcattcgtga tctcggtttg gcggatgaaa tgaatgcagc aggtttccct
     3661 gataaacctg gtgtgaatgt gattggttcg ctatcaaaga atgagttctt tattcctatt
     3721 ttagcgccga cttggcaggt acgccgtagc gactttgaca acatgctaaa gcgaagagcc
     3781 ctagagcatg gcgttgaata tcagcaaggc ttagttaaag acgtgatcaa gcatgaagaa
     3841 aaagtggttg gcgcgattta taaagccgat ggcgttgagc atcaagtacg ctcaaaggta
     3901 ctggtagatg ccagtggtca aaataccttt ttatcgcgca aaggcattgc cggcaaacgt
     3961 gaaattgagt tcttttcaca gcagatcgct tcttttgcac attataaaaa tgttgagcgc
     4021 gatttaccgc ctttctcaac gaataccacc attttatatt caaagcagta tcactggtcg
     4081 tggatcattc caatctcgcc agacacagac agtctaggaa tagtgatccc gaaagacttg
     4141 tactacaaag agtgtaaaaa cccagatgat gccatcgaat gggggatgga gcatatcagc
     4201 cctgagatca gacgccgttt caaaaatgca gagcgcgtag gtgagtcgca atctatggca
     4261 gacttctctt accgtattga gccgtttgta ggagatggtt ggttatgtat cggtgatgcg
     4321 catcgattct tagatccaat tttctcttat ggtgtgtcat tcgccatgaa agagggcatt
     4381 aaagccgcgg atgccattaa gcgtgctatc gatggtaacg attggaaaac gcctttctac
     4441 gaataccgcg attggagtaa tggcggtcag caaattgctg cagatcttat tcgttatttc
     4501 tggatctacc caattttctt tggctatcaa atgcaaaacc ctgatttacg cgatgaagtt
     4561 atccgtctat taggtggctg ttgctttgat tgtgaaggat ggaaagcacc gaccatcttt
     4621 agaaatgcca ttgaagaata cgaccgcaaa caaatggctg gttgaggata gatcatgaac
     4681 tttgcgtgga atgaggatca gctcgcatta catgagcacg ccttgagctt tgcacgagat
     4741 ctcgactttt cgaagatctc tgctggcgag tttccaatgg aagcttggca atcactgggt
     4801 gagtttggct actacggttt acccgtagat agccgctact cgccttgtgg cagtggtttt
     4861 gacattttaa ccacagttaa aattttagag ggtcttggtc aaggttgtgc agataccggc
     4921 attttatttg ccggtgctgc acacaccttt gcttgcgtta tgccgattta tgaacatggt
     4981 agtgaaacac ttaaacatga attattaccg gcacttacca gtggtgagaa aattgcggcg
     5041 aatgccattt ctgaagctga agcagggtca gatattggca aattaacgac caaggcaacc
     5101 agagagggtg attactacat catcaatggt gccaagtctt atgtgactaa tggcagtgtg
     5161 gccgactact ttgttctcta tgcgaccact aacccaaagt ttggctactt aggacaaaca
     5221 gcctttgttg tgcctgctga tttaccaggc attgaagtgg gaaaaagcta caaaaagtta
     5281 ggacttgact cggcgcagct caatcaagtg ttttttgatg aggtaaaagt gcataaggac
     5341 tatgtactag gcagtgaagg gcagggggcg cgtatttttg ccgcttctat ggattgggag
     5401 cgttgttgtc tatttgcttt attcattggt gtgatgcaga acgatttaga gcgctgcatt
     5461 gagtttgcca atacccgtaa gcagggcgat aaaaccatca gtaaatttca ggcggtgtca
     5521 catcgtattg ctgatatgga agtacgttta caaagtgcgc gcttattgtt gtatttcgcg
     5581 gcttggcaaa agtcgcaaga tgttgataac accaaagcag tggcatcaag taaattagcc
     5641 atcagtgaag catttgtgca atcgggtatt gacgctattc gcattcatgg cgcggccggt
     5701 tacttacaag aaagcggcat agatagcagc attcgtgatg ccctagggtc ggtgctgttt
     5761 tcaggtacgt ctgatattca acgagagctc atctgtaaca ggttgggcct gctatgaagt
     5821 tgctcaatca agttttaact caaggcttag tgcgcgctgg tgaacaagca ctggcggtga
     5881 gcgatagcac agagtcgtat agttatggtg aattacaact cagggcaact caaattgcag
     5941 tgctgctgca aaaaatcggc gttaaagcgg gggatagagt agggctttgg tgtgataagt
     6001 catgtcgagc tttggctggc atgttggcgg tgagtaaact caatgccgtt tatgtaccaa
     6061 tcgatccaat gaatccgaaa gtgcgtgtgg atattattcg tcaagactgc gacatgaagg
     6121 tattgatcac caataaagcg aagtttagtc aatatcagcg cggtgataat gctttatgcg
     6181 actttgtggt attagacgaa gatgcgctaa ttagctctgt attacatgta cacacgtggc
     6241 agcatatttc tgctcagcaa ccaagccagc tcaacttacc aaaagagggt aaaagtacag
     6301 atttggcgta tatgctgtac acatctggct caactggtgt gccaaaaggc gtcaagatca
     6361 gtcatgaaaa tgcccttgcc tttattgaat ggtctacgaa taaactcaat gcaacagtgc
     6421 aagatcgttt tgccaatcat gccccctggc attttgattt atcagtgctg gatatttatg
     6481 tggctttgtt atcaggcgca agtgttcatt tggtgtctga aatcgacagc tatgtggcac
     6541 aaaaattagt gtcatttgtc gcccaaaagc agatcagtat ttggtattcc gtgccgaccg
     6601 cattggtgat gatgctagaa gctgaggctg actttgcaga gcgttgccag cagcttagag
     6661 ccattatttt tgccggtgaa gcctttgcga ttaaaccgct gaaacaactt cgcgaagcgt
     6721 ttcccgatgc cgagctttac aacttttatg gcccaaccga aaccaatgta tgtagttact
     6781 acaaagtccc tcgggtagta ccagacaagc tgcctatagg ttacccagcc agtaatgctt
     6841 tgctttcaat ttgtgatgaa caaggtaaag cactgcctgt gggcgagcaa gggtttgtta
     6901 ctgtaattgg cccgactgtt ttcagcggtt attggggacg cgcagaactg caatcagaag
     6961 cttataacac aggtgatatc ggctatttca atgagctggg agagttgatg tatgtcggtc
     7021 gtcaagatca tatggtgaaa gtgaatggtt atcgggttca tcttggtgaa gttgaacgcg
     7081 ctatttacca gcacccagca gtgaaagagt gtttagttga gctcagcagc caaaaaacac
     7141 tccatgcgca tattgccgta gagggtgatg cgccatcttt attaacgcta aaaattcact
     7201 gtgccaaacg tttacctaaa tacatgttgc ctgagcaggt gatagtgcac gatgcactgc
     7261 ctcgtaatcg caacggaaaa ttatcgcgtc aatgcttatc tgggttgtct gctcaatcgg
     7321 gatctgaaaa taacacccag atcacgatga gcgaggcgta acacgcttgc atctgcaggc
     7381 acctcctagt taaacaagga atgaaaaatg agaaaaaaaa tcgcagtcat cggtgctggt
     7441 ttatcaggca ttgccgccat caaacagctt acagacggag gccatgaggt cgtctgtttt
     7501 gaaaaagcag aaagctttgg tggtgtattt gccgataaaa aaatctatga agatttgcac
     7561 ctaaccattt ctaattactt tatggcgtat tcagattatg taccgagcca gcaaaagctg
     7621 aagttctggt ctaaaaaaga atatgttaat tacttaggcg aataccttgc ccgcttcgaa
     7681 ttaggtcaat acattcatta cgatcacgaa gtacgaaagg tagaaaagca agcgggaaaa
     7741 tggcaggtca ccacgaaaca tggcatggct gaacaaactg acacctttga tatggttgcc
     7801 gtttgctcgg gtcatttcca aaagccaaaa atgcctgagc ttgctggttt agatatgttc
     7861 gagggcgaaa ttgagcactc gaatgattac cgcgataagc acaaatatgc cggtaaacgt
     7921 gtgttatgtg tcggtttggg ggaaagtagt gccgacatta cttctgagat ttcagaagtt
     7981 gcgagtaaat gtatcttatc attgcgccgt tacccagcgg tggctccgcg ctatatggcg
     8041 ttccaagaag atccttactt caccatagat accagctggt taacatcacg catcgtgaac
     8101 aaattaccac atcgttatca tggtgggatc acgaaaggca ttttcactaa gtatgtgaat
     8161 agccgtaacg atcatgtacg gatcagaggc gagtggctga aaaaatcagg cccttctcac
     8221 catcaggccg tcactaaaaa tgagcgacta ttcaggccaa tcgccgatgg taaagttgtg
     8281 cctaacattg gtggcattgt gcgttttgaa aaaaacgctg tggtgtttca agatggcacg
     8341 cgtgaagaaa ttgatgctgt ggtattttgc accggctatc agctgagctt cccattctta
     8401 gatgtcagca tcagcaatat gcgtgacttg tataaacaaa tgtttattcc tgaaatgggt
     8461 gactcactgt cgtttatcgg ttttgttcgt ccgcaacaag ggggtattcc agtcattgct
     8521 gaaatgcagt gtcgttactt atcgcaactg gccagtggtg agaaatcatt accaccgcta
     8581 tcagaaatgg tcgatatcat taaatacgat actgagcact ggcagacgga gtataagatc
     8641 accccacatg tggcctcttt ggtgaactac tgccattaca tggattctat ggcaaagctg
     8701 gtcggctgca tgcctgagat cccaagcttg tttaaagatc cattgttgcg cgtaaaactt
     8761 ttgcataacc cgcagtttgc agcgcaatac cgtttagatg gtccaaataa aatgacgcat
     8821 acagcgcgat cgtttttatt aggctttcca aatatcagtt cttggccacg catcattcat
     8881 tttgagttgg ctttgatcac ccaaaaacta ctctcacgtt tacgccttga tggcttacgt
     8941 gagcttagta aatagctgct cagggacggg aaaacgttat gatccgacta accgatgagt
     9001 gcttgtgtaa tctagatgat gcattgctac cacaccgctt taacttaagt cgtttaagcc
     9061 atttgcaaaa aatcatttta ggtaccgatg gcacagtaac ccaattaatc gaaagtattg
     9121 tcgatgaaaa gttggtggtg aataaactgt ttgaagccga tgttgatagt gacacgatcg
     9181 ccacaacgtc gagtgaacaa caagctgaaa tacattgcca aaggcgggtt attttattgc
     9241 aaggcgagac ctcaggcttg ccttatctat atgccgactc cttggtttat cacggcaaca
     9301 tgaacgtgaa tttttctcgt gccctgactc attctcagat ccccatcggc aaagcttggg
     9361 aaaagtttca ggtagaaact tataaaacct tgctgtcatg ggggtttgaa tatgccaatg
     9421 agctagcacc tcactttgcg atttcaagcc atgacttggt gttatatcgc acctatttag
     9481 tgttctctca gggtaaaaag atcttccgga tcaccgaaaa atttcctttc gtttggtatc
     9541 tgcaatcaga gcaattcaca gcgagtgcag cgaccactgc aacgccaact ttagtcagcg
     9601 gaatttgagc aatgaaaaat acagacagcg tcgtggcgca accacaaatc aatttagata
     9661 ttaacaaact gactccgagt attccccaca tcccatttaa cagcgaagac ggacagctgt
     9721 ttttttgggg ggaggattac cacctaatta tcgaatctct atcaaagaaa tatggtgata
     9781 tctactcggt tgaaacagcc aagggcaatt tggtggtatt aaacggctat caatacatta
     9841 aagaagcatt ggttacacaa aatgacgtgt tcaatgtacg agccgattat aaaattcttc
     9901 aggtggcacc acaaaagcac tttttagagc tagaagctgg cgagctttgg caggtacacc
     9961 gtaaggtgtt tgcccatgcc atgcgtgaat attttgccac gcgctgggat agcattgagc
    10021 aatggatgac cactgagatt gaagatatcg cagcggtatg gcaagccgac gagggcaagc
    10081 catttgaccc aaatcgcgaa gtatcgatta agcttgctag ctttttacac aaggtcatgt
    10141 ttgatgatcg atttggtgag tttgaaaaag gtctgtttga cgagaaaagc ttaagttggt
    10201 taccaaatgg ttttattaac agcacgcgtt acgagttgat gcctactgaa ctacaagaga
    10261 aatattacgc tcagtatggt gatgcgattg aaaaatttag cggcaaccta aacgcccttg
    10321 atcaatacgt gtcattcaat gttgatcgtc gtaaagccga ttataaaccg ggcgagtatc
    10381 gtgatttatg tgattatttg tttgctgcaa atgacagtgt cgatgagcaa acgaaagccg
    10441 ctttgaacat tgatgaaaaa gaaattgtca tcggttcact gactcaagtc gcgggagctg
    10501 gtggcggtgt tggtgcattt gcgatgcgct gggccttgtt atacttggct gcttttccaa
    10561 aacagcaaca gcgagtacaa aaagaactcg atgaagtggt gggtaaaaac gcggtgccat
    10621 tgaataagca caaagcggat ctgccttaca cacaggcatt tattagtgag gtattgcgtc
    10681 attgttcaat tacatctatg ccagcggcca actatgcaac cagcaaagac acgttattag
    10741 acggttattt tcttgccgaa ggcacgccat tggtgattaa taattacagt attacccgag
    10801 atgaaacgct gtgggaaaac ccgcatgaat ttatgcctga acgcttttta gatgaagatg
    10861 gcgagttgag caaaaaacaa ttgggtaaag ctttcccatt tggtttaggt cagcgccgct
    10921 gcttgggtga acattttggt aagtacatca ttcatacctt gtttgctcag ttagcgcata
    10981 agtttgaatt tagtctgcct gaagggaagc aagcaaatct taaagcgatt tcaggggtgt
    11041 ttttagtacc tgaagaagtc gaaatcgtgg ctaaatcgcg caacgcctaa
//
