LOCUS       BGC0000892             13187 bp    DNA     linear   BCT 10-SEP-2019
DEFINITION  Burkholderia gladioli BSR3 chromosome 1, complete sequence.
ACCESSION   BGC0000892
VERSION     BGC0000892
KEYWORDS    .
SOURCE      Burkholderia gladioli BSR3
  ORGANISM  Burkholderia gladioli BSR3
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Burkholderia.
COMMENT     Source DNA available from Ingyu Hwang (ingyu@snu.ac.kr).
            ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 14:23:14
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             complement(1..1554)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1076:RND efflux
                     system, outer membrane lipoprotein, (Score: 356.9; E-value:
                     2.4e-108)"
                     /gene_kind="transport"
                     /locus_tag="bgla_1g20130"
                     /product="RND efflux system, outer membrane lipoprotein,
                     NodT"
                     /protein_id="AEA60652.1"
                     /transl_table=11
                     /translation="MSRTPDHSFARPRRPGRPALARPAAMAAAALAASLLGGCTVGPDY
                     RPADSPLAPNYASQDALRTLQARPAAEPVALDAWWLGFHDPVLTRLIERALAQNLDLAA
                     AEARVAQARAAAADAGAQRLPRLDVEGTAARQRQSLESPLGRIGSALPGYDRNQGYTQL
                     GLGASWELDLAGGLRRQAQAASAEAEAAEALHAGSRVSVAADAADAYFRLRGLQARIAI
                     VEQQIEADRHLVSLVRDRVGNGVATRREQAEAEARLAQTRSQRPPLLAERARQANRLDI
                     LMGSAPGTYTAQLGAPAADPVVPGLPAGIGPDTLLRRRPDVVAAERRLAASNAGVGAAL
                     AEYYPKLSLSGILGFEALDGPLFKSAAFQPGALAGLRWRLFDFGRVDAEVAQARGRHAE
                     ALAVYRQAVLRAAEDVENAVTDWAQIGAQRDELARQVEAEALAQRAARDAYAQGDASLV
                     EVLVEDQQWLAARGEQARLNADYARAAVATFRSLGGGWTPPETAPAAWAASSRQDPPAP
                     "
     subregion       1..13187
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000892"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..13187
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            complement(1..1554)
                     /locus_tag="bgla_1g20130"
     gene            complement(1566..3206)
                     /locus_tag="bgla_1g20140"
     CDS             complement(1566..3206)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1005:Drug
                     resistance transporter, EmrB/QacA (Score: 349.6; E-value:
                     3.3e-106)"
                     /gene_kind="transport"
                     /locus_tag="bgla_1g20140"
                     /product="transporter"
                     /protein_id="AEA60653.1"
                     /transl_table=11
                     /translation="MSELAKRPAGAAAEGAPGAAGAGAASGPPPAAAHPPAWPFAVMCV
                     GMFIALLDIQIVASSLQEIGGGLSAAQDQIGWVQTAYLIAEITVIPMSGWLTRVFSTRR
                     LFAGSALGFTAASLLCGFAWNIESMIVFRAIQGVLGASMIPTVFTSSFHYFDGKRRVIA
                     AAVIGTIASLAPALGPIVGGFITDTANWRWLFYVNLVPGLLVALGVSLAADIDRPDHAL
                     LKGADYIGIALMAVFLGTLEYVLEEGARWNWLDDPVIRRCAVISACAGSVFVARCLSID
                     NPIVDLRAFGNRNFTIGCILSFLTGVGAFSSIYLTPLFLGYVRGYDAWQTGVAMMPTGI
                     AALVGVPVYVMFARKTDLRWLMMFGMAMFGLSMWDFRFITHDWGNQQLLWPQLIRGFPQ
                     VFAIAPAVTLGLGSLPPERLKYASGLFNMMRNLGGAVGIAIVGAILNNRTNLHFTDIAS
                     RLTAANQPMNKMLGTLEAQLGPALGSAQAGAAASLRELHDIALREAQTMAYGDALTTIM
                     IGFMFATLIVPLMRKVVPPPPPDSSKNAH"
     gene            complement(3206..4294)
                     /locus_tag="bgla_1g20150"
     CDS             complement(3206..4294)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1029:RND family
                     efflux transporter MFP subunit (Score: 175.9; E-value:
                     1.8e-53)"
                     /gene_kind="transport"
                     /locus_tag="bgla_1g20150"
                     /product="secretion protein HlyD"
                     /protein_id="AEA60654.1"
                     /transl_table=11
                     /translation="MSENQTGATALPGAADGKPKRKRKLVLPVLIVLLVLLAGYAIYWW
                     LHGRFYETTDDAYVGGNITVISPHVAGYVSELLVEDNQRVRAGQPLLRLQPGDFAAALD
                     AAKANEAAMRASRAQLLARRTLQQTLIDQAGAEVAEKSAALSFARTDAARYRNLLSTAA
                     GTRQSEERSSAALKQAQAQLDSASAKLREARQQVGVLDSQIAGADAAILQAGAAQRTAE
                     LNVGYTELRAPIDGYVGNRAIHVGSYVTPGTQLMSVVAAKGLWVDANYKEDQLRHMQAG
                     QRAKICADVQSSRCFEGRVHLLAPATGAVFSVIPPQNATGNFTRIVQRVPVRISFEAAD
                     GVLGVLRPGLSTTVTVDTHGAS"
     gene            complement(4531..5727)
                     /locus_tag="bgla_1g20160"
     CDS             complement(4531..5727)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007:cytochrome P450 (Score: 357.3; E-value:
                     1.7e-108)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="bgla_1g20160"
                     /product="cytochrome P450"
                     /protein_id="AEA60655.1"
                     /sec_met_domain="p450 (E-value: 2.3e-27, bitscore: 85.4,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQYSDLATPDFLKNPYPVFDAMRAEGQLVKLAPKLYASSHYGFGE
                     KLLLDRRFGKGIVEAVKARYGEGVIDQPPFRTFRSMLPGLNPPKHTNLRALLMKSFNAR
                     QVEKFREASYTISNQLIDRLVKEPQGDLVTGFAFLLPMQTMCTILGVPLSDGAMFKRAA
                     DRAAGALNVTPLNAEQLEESRKSAIELETYFAKVLAERRKEPGDDLISQMILAEEDGQR
                     LTDDEIVANLCFLFVAGHETTENMIGNTLIALQRHPEQRERVKADLSIMPRVVTEALRY
                     DSSVQIAQRVALEEVELEGQTIQRGDLICVFLGGGNRDPEKFENPDRLDIDRENVRPLS
                     FGGGLHYCLGARLALLEIAAALEVVYTRLPNLHLTNLDALPYRRNNALRGVDSLLGTW"
     gene            complement(5777..5962)
                     /locus_tag="bgla_1g20170"
     CDS             complement(5777..5962)
                     /codon_start=1
                     /locus_tag="bgla_1g20170"
                     /product="rubredoxin"
                     /protein_id="AEA60656.1"
                     /transl_table=11
                     /translation="MSELTVEYRTWMCLICGYLYDERSGDPNAGIAAGTRWEDIPDDWR
                     CPECDVPKLDFEMVSI"
     gene            complement(5980..6852)
                     /locus_tag="bgla_1g20180"
     CDS             complement(5980..6852)
                     /codon_start=1
                     /locus_tag="bgla_1g20180"
                     /product="hypothetical protein"
                     /protein_id="AEA60657.1"
                     /transl_table=11
                     /translation="MLYPELYRSLERARWDMQHDVDWDRFDASLLSDEQALSIKMNAIT
                     EWAALPATEMFLRDNVDDSDFSAFMSIWFYEEQKHSLVLIEYLRRFRPELAPTEDELHA
                     VRFPFDPAPALETLTLHFGGEIRLNHWYRCAAHWHTEPVIRQIYELISRDEARHAAAYL
                     KYMRRALDRHGDAARLAFAKIGTLMASANRAAKAIHPTNLHVNRTLFPNDTVQSRLPDP
                     EWLGRWLDTQIRFDRDWENRVADRILHNLSILLDRPLLNVKDLHKLRKQLHQNLEGASA
                     VAAEPALQH"
     gene            complement(6868..7842)
                     /locus_tag="bgla_1g20190"
     CDS             complement(6868..7842)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="bgla_1g20190"
                     /product="delta 12 desaturase"
                     /protein_id="AEA60658.1"
                     /sec_met_domain="PF00561 (E-value: 1.2e-12, bitscore: 37.6,
                     seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="Abhydrolase_6 (E-value: 8.5e-11, bitscore:
                     32.5, seeds: 455, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSNTSESSLPHGAAAAAAPAPSPARAASPAMSFGKRVLKGYVGAL
                     GAVSPAAAARRATDLFGYTRTFRKTPPKDMSPLGARRFEIRGVDGVTHGHLWGKGERTV
                     LLVHGWGADSATMFSFVPKLQKAGFRVAAFDAPAHGVSPGTVTTMTAFKNAVKGAIESL
                     GGVHGIVSHSLGSIASTGAMAELGPDSVDGIVMLAPPCTLPAVIDRWSGDFLRLAPSVM
                     AAMYAELHRRNGVPPQHWDIGALGRGLRTRIFVMHGPNDKIVPICESENIAAALPHVRF
                     ERVEKVGHVRILSDARVIERATQFLAIGGEPAANAPSLAPVTG"
     gene            complement(7901..9001)
                     /locus_tag="bgla_1g20200"
     CDS             complement(7901..9001)
                     /codon_start=1
                     /locus_tag="bgla_1g20200"
                     /product="Fatty acid desaturase"
                     /protein_id="AEA60659.1"
                     /transl_table=11
                     /translation="MATIQLPQQQTPGFRDFVQSIKSLDGERLADAIPRELYEPRLARG
                     LLGFFASTALYVGAVIGVHYAPHWSLWIPLWLLAGLGGWGLFCIAHDCGHNSFSKSRRF
                     NFALGQIALFPLLYPFHGWRHMHNLHHANTNSLEMDTDWRPVLPEQYRRMGAWEKFVYR
                     STRSWLFWLGTVNYQRHSGFKPSMFPKREARNDVRRSVAVTVLFAAVYLPILVWFTGWQ
                     GFLLYFLGPWLGIHAWFSTTTMMHHISDELPFLTREHWSLNGSRLMMTTDYEYPKWLHF
                     FTHNISVHAAHHVVPVIPFYNLPKAREALKRAYPGSIRQKPFTLGEVWKVIRACHLYDP
                     VHGYYRSFEALATPPAATPPRADSAI"
     gene            complement(9023..9346)
                     /locus_tag="bgla_1g20210"
     CDS             complement(9023..9346)
                     /NRPS_PKS="Domain: ACP (29-100). E-value: 2.1e-21. Score:
                     67.9. Matches aSDomain: nrpspksdomains_bgla_1g20210_ACP.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1047:phosphopantetheine-binding domain-containing
                     protein (Score: 72.6; E-value: 3.9e-22)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="bgla_1g20210"
                     /product="polyketide synthase"
                     /protein_id="AEA60660.1"
                     /sec_met_domain="PP-binding (E-value: 5.9e-18, bitscore:
                     55.0, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAFMSSLFSKIGFIEEKRSSTPLTEAGIHQWLVERLARQLNVPIA
                     QIDPARSFESYGLDSLVALQVAGDLEKLLEQRLSPALLFEHQNIDDLASYLAAELATGE
                     IKA"
     aSDomain        complement(9047..9259)
                     /aSDomain="ACP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_bgla_1g20210_ACP.1"
                     /evalue="2.10E-21"
                     /label="bgla_1g20210_ACP.1"
                     /locus_tag="bgla_1g20210"
                     /protein_end="100"
                     /protein_start="29"
                     /score="67.9"
                     /tool="antismash"
                     /translation="QWLVERLARQLNVPIAQIDPARSFESYGLDSLVALQVAGDLEKLL
                     EQRLSPALLFEHQNIDDLASYLAAEL"
     gene            complement(9409..10383)
                     /locus_tag="bgla_1g20220"
     CDS             complement(9409..10383)
                     /codon_start=1
                     /locus_tag="bgla_1g20220"
                     /product="fatty acid desaturase family protein"
                     /protein_id="AEA60661.1"
                     /transl_table=11
                     /translation="MTQLDLTPAPTLPDDQPVRGVSVLDARGARIKAMTAFGVMTIPLA
                     GTLAAAWLAWAGYATRVDAWLFAAMYVLHMAGVSIGYHRFLAHRAFRTSPLFRALILIC
                     GSMAAQGPILFWVTTHRRHHAYSDRPGDPHSPNLLGESRAERLRGLWHAHMPWMMSPEM
                     SSWSYFAQDILKDRSLLFFNRTYFYWVLLGLAIPAVAGGLITASWMGALCGLMFGGFAR
                     MFIANQFAWCVGSICHRYGSRPFDTRDHSTNNWIVAFLTFGEGLQNNHHAFPAWYRHGV
                     HWWEPDLSGWMLALFGRLGIVEELRSPSRDVIERVRKREPGGA"
     gene            complement(10409..11338)
                     /locus_tag="bgla_1g20230"
     CDS             complement(10409..11338)
                     /codon_start=1
                     /locus_tag="bgla_1g20230"
                     /product="fatty acid desaturase family protein"
                     /protein_id="AEA60662.1"
                     /transl_table=11
                     /translation="MDIQTQTSPSRPAPLADRTGATERRLAWLTVGIPAVGALAAVALA
                     IVHGIGWLEIGLLAVMYLVTALGVEAGLHRFFSHQAFKAGPVVTALFAIFGSMAAQGPI
                     LFWAATHRMHHAFTDQEGDPHSPRLHGEHLGGRLRGIWHAHVGWLFTLRRQNWSQFTPD
                     LLRDRWVVRLNRLYFLWIALGLALPTLAGWLIGGTGYAALTGFLWGGLLRIFLVDQATW
                     AINSFAHSFGPRPNRTRDQSRNVFWLALPAVGGGWHNNHHAFPALARTGQRFWQLDLSG
                     GLIELLAVFGLVWDVRRPAPERSRATQS"
     gene            complement(11355..13187)
                     /locus_tag="bgla_1g20240"
     CDS             complement(11355..13187)
                     /NRPS_PKS="Domain: CAL_domain (65-506). E-value: 1.9e-112.
                     Score: 368.0. Matches aSDomain:
                     nrpspksdomains_bgla_1g20240_CAL_domain.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002:AMP-dependent synthetase and ligase (Score:
                     380.9; E-value: 1.3e-115)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="bgla_1g20240"
                     /product="pyoverdine synthetase"
                     /protein_id="AEA60663.1"
                     /sec_met_domain="AMP-binding (E-value: 6e-94, bitscore:
                     304.5, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHLEVASPALDALAASHSRHRAAATLAEHALDTLADVLRLRAAT
                     LPGQTAFIHLVDGEGETRTVSYAELQLQAGTIARHLAARGVAGKRVLMLFEAGIDYIAA
                     LFGVWMAGAVAVPSFPPVGTRALGRLGAIARDSQPELILTDSRFTRLRERVLALLPDGF
                     PEEAWTEADALFRVDTHAYAYNPADAGLALPVLDREALALLQYTSGSTSDPKGVMLTHG
                     NLLSNCHGASRWMGGPRERVGCTWLPPYHDMGLMGGILQPIYEGFPTVILSPGHFVQQP
                     LRWLAALSQYRVTTTIAPNFAFDLCCNAIGDDVLPLLDLSTVEAIYCGAEPVRRRTFER
                     FAERFARCGFRAEAFGPCYGLAEATVFVSGKVDGAAPAFVELDQDGLARGEVREIAADH
                     PRAQALASCGAPAPGHDVRIVGPDGRALPDGAIGEIWVGGPNIGIGYWNQPERSEETFY
                     ARLEGSAQRYMRTGDLGFLRAGELYVTGRIKDLIIHAGRNLYPQDIELLVEELDARIRP
                     NGVAAFSIDDGQGERIAVVAELRRGEKVAEGELQTLREAIVERVTQQLGAAPHLIHFAP
                     MGAIPLTTSGKIQRYATRHALLTQSLASYPHARA"
     aSDomain        complement(11670..12992)
                     /aSDomain="CAL_domain"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_bgla_1g20240_CAL_domain.1"
                     /evalue="1.90E-112"
                     /label="bgla_1g20240_CAL_domain.1"
                     /locus_tag="bgla_1g20240"
                     /protein_end="506"
                     /protein_start="65"
                     /score="368.0"
                     /specificity="Minowa: NH2"
                     /tool="antismash"
                     /translation="SYAELQLQAGTIARHLAARGVAGKRVLMLFEAGIDYIAALFGVWM
                     AGAVAVPSFPPVGTRALGRLGAIARDSQPELILTDSRFTRLRERVLALLPDGFPEEAWT
                     EADALFRVDTHAYAYNPADAGLALPVLDREALALLQYTSGSTSDPKGVMLTHGNLLSNC
                     HGASRWMGGPRERVGCTWLPPYHDMGLMGGILQPIYEGFPTVILSPGHFVQQPLRWLAA
                     LSQYRVTTTIAPNFAFDLCCNAIGDDVLPLLDLSTVEAIYCGAEPVRRRTFERFAERFA
                     RCGFRAEAFGPCYGLAEATVFVSGKVDGAAPAFVELDQDGLARGEVREIAADHPRAQAL
                     ASCGAPAPGHDVRIVGPDGRALPDGAIGEIWVGGPNIGIGYWNQPERSEETFYARLEGS
                     AQRYMRTGDLGFLRAGELYVTGRIKDLIIHAGRNLYPQDIEL"
     CDS_motif       complement(11817..11906)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_bgla_1g20240_0002"
                     /evalue="1.30E-12"
                     /label="NRPS-A_a6"
                     /locus_tag="bgla_1g20240"
                     /protein_end="457"
                     /protein_start="427"
                     /score="39.9"
                     /tool="antismash"
                     /translation="PDGAIGEIWVGGPNIGIGYWNQPERSEETF"
     CDS_motif       complement(12525..12584)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_bgla_1g20240_0001"
                     /evalue="1.20E-07"
                     /label="NRPS-A_a3"
                     /locus_tag="bgla_1g20240"
                     /protein_end="221"
                     /protein_start="201"
                     /score="24.0"
                     /tool="antismash"
                     /translation="ALLQYTSGSTSDPKGVMLTH"
ORIGIN
        1 tcatggggcc gggggatcct ggcgcgagct ggcggcccat gccgccgggg cggtttccgg
       61 cggcgtccag ccgccgccca gcgagcggaa ggtggccacc gccgcgcgcg cgtagtcggc
      121 gttcaggcgc gcctgctcgc cgcgcgccgc gagccattgc tggtcctcca ccagtacctc
      181 gaccaggctc gcgtcgccct gcgcataggc atcgcgcgcc gcgcgctggg ccagcgcctc
      241 ggcttccacc tggcgcgcga gctcgtcgcg ctgcgcgccg atctgcgccc agtcggtcac
      301 cgcgttctcc acatcctcgg ccgcgcgcag cacggcctgg cggtagacgg cgagcgcctc
      361 ggcatggcgc cccctggcct gcgccacctc ggcatcgacg cggccgaagt cgaacaggcg
      421 ccagcgcagc ccggccagcg cgccgggctg gaacgcggcc gacttgaaca gcggcccgtc
      481 gagcgcctcg aagccgagaa tgcccgacag cgacagcttc gggtagtact cggccagcgc
      541 cgcgcccaca cccgcgttgc tcgccgccag ccgccgctcg gccgccacca cgtcggggcg
      601 gcggcgcagc agcgtgtccg ggccgatccc ggccggcagg cccggtacca ccgggtccgc
      661 ggccggcgcg cccagttgcg ccgtgtaggt gccgggtgcc gagcccatca ggatatccag
      721 ccggttggcc tggcgcgctc gctcggccag caagggcgga cgctgcgagc gcgtctgcgc
      781 gagccgcgcc tcggcctcgg cctgttcgcg ccgtgtcgcc acgccgttgc cgacgcgatc
      841 gcggaccagc gagaccaggt ggcggtcggc ctcgatctgc tgctcgacga tggcgatgcg
      901 cgcctgcagg ccgcgcagcc ggaaataggc atcggccgcg tcggccgcca ccgagacgcg
      961 cgagccggca tgcagggcct cggccgcctc ggcctcggcg ctggcagcct gggcctggcg
     1021 gcgcaggccg ccggccaggt cgagctccca gctcgcaccg aggccgagct gggtatagcc
     1081 ctggttgcga tcgtagccgg gcagggccga gccgatccgg cccagcggcg attccagcga
     1141 ctgccgctgg cgcgcggccg tgccctcgac gtcgagcctg ggcagccgct gcgcgccggc
     1201 atcggcggcc gccgcgcgcg cttgcgccac gcgcgcctcg gcggcggcca ggtcgaggtt
     1261 ctgcgccagc gcgcgctcga tcagtcgcgt gagcaccgga tcgtggaagc cgagccacca
     1321 ggcatcgagc gcgaccggtt cggccgcggg ccgcgcctgc agcgtgcgca gcgcgtcctg
     1381 gctggcatag ttcggcgcga gcggcgaatc ggccggccgg tagtcggggc cgaccgtgca
     1441 gccgcccagc agcgacgcgg ccagcgcggc ggcggccatt gccgccggcc gcgcgagagc
     1501 cgggcggccc ggccggcgcg gccgcgcaaa cgaatgatca ggagttcgtg acattgggag
     1561 gcgcctcagt gagcgttctt cgacgaatcg gggggcggcg gcggcaccac cttgcgcatc
     1621 agcggcacga tcagggtggc gaacatgaag ccgatcatga tggtggtgag cgcgtcgcca
     1681 taggccatgg tctgcgcctc gcgcaacgcg atgtcgtgca gctcgcgcag cgaggcggcc
     1741 gcgccggcct gggcggagcc gagcgccggg ccgagctggg cctccagcgt gccgagcatc
     1801 ttgttcatcg gctggttggc ggccgtcagg cgcgaggcga tatcggtgaa atgcaggttg
     1861 gtgcgattgt tgaggatcgc gccgacgatc gcgatgccca ccgcgccgcc caggttgcgc
     1921 atcatgttga acaggccgct ggcgtacttg agccgctcgg gcggcaggct gcccaggccg
     1981 agcgtgaccg ccggcgcgat cgcgaacacc tgggggaagc cgcggatcag ctgcggccag
     2041 agcaactgct ggttgcccca gtcgtgggtg atgaagcgga agtcccacat cgacaggccg
     2101 aacatcgcca tgccgaacat catcagccag cgcaggtcgg tcttgcgcgc gaacatcacg
     2161 tagaccggca cgcccaccag cgcggcgatg ccggtcggca tcatcgccac gccggtctgc
     2221 caggcgtcgt agccgcgcac gtagccgagg aacagcggcg tcaggtagat cgacgagaag
     2281 gcgcccacgc cggtcaggaa ggacaggatg cagccgatcg tgaagttgcg gttgccgaag
     2341 gcgcgcaggt cgacgatcgg gttgtcgatg ctcaggcagc gcgccacgaa cacgctgccc
     2401 gcgcaggccg agatcaccgc gcagcggcgg atgacggggt cgtcgagcca gttccagcgc
     2461 gcgccttcct cgagcacgta ttcgagcgtg ccgaggaaca ccgccatcag cgcgatgccg
     2521 atgtagtcgg cgcccttgag caaggcgtga tcgggccggt cgatgtcggc cgccagcgag
     2581 acgcccagcg ccaccagcag ccccggcacc aggttcacgt agaacagcca gcgccagttg
     2641 gcggtatcgg tgatgaagcc gccgacgatc ggccccagcg ccggcgcgag cgacgcgatg
     2701 gtgccgatca cggcggccgc gatcacgcgc cgcttgccgt cgaagtagtg gaacgaggag
     2761 gtgaacacgg tggggatcat cgaggcgccc agcacgccct ggatcgcgcg gaacacgatc
     2821 atgctctcga tgttccaggc gaagccgcat agcaggctgg cggccgtgaa gccgagcgcc
     2881 gagccggcga acaggcggcg cgtggagaac acgcgcgtga gccagcccga catcgggatc
     2941 acggtgatct cggcgatcag gtaggcggtc tgcacccagc cgatctggtc ctgtgccgcg
     3001 ctcaggccgc cgccgatctc ctgcagcgag gaggccacga tctggatgtc gagcagcgcg
     3061 atgaacatgc ccacgcacat caccgcgaac ggccaggcgg gcggatgcgc ggcggccggc
     3121 ggcgggccgg aagcagcgcc ggcaccggct gcgccgggcg cgccctcggc cgccgcgccg
     3181 gcggggcgct tggcgagctc gctcatcagg aggctccgtg ggtgtcgacc gtcacggtgg
     3241 tcgagaggcc cgggcgcagc acgcccagca cgccgtcggc cgcctcgaag ctgatccgca
     3301 ccggcacgcg ctgcacgatg cgcgtgaagt tgccggtggc attctgcggc gggatcacgc
     3361 tgaacaccgc gccggtggcc ggcgccagca ggtgcacgcg gccctcgaag cagcgcgagg
     3421 actgcacgtc ggcgcagatc ttggcgcgct ggccggcctg catgtggcgg agctggtcct
     3481 ccttgtagtt cgcgtcgacc cacaggccct tggcggccac caccgacatc agctgggtgc
     3541 cgggcgtcac gtagctgccc acgtggatcg cgcgattgcc cacgtagccg tcgatcggcg
     3601 cgcgcagctc ggtgtagccg acgttgagct cggcggtgcg ctgggccgcg ccggcctgca
     3661 ggatcgccgc atcggcgccg gcgatctggc tgtcgagcac gcccacctgc tggcgcgcct
     3721 cgcgcagctt cgccgaggcc gaatcgagct gcgcctgggc ctgcttgagc gcggcgctgg
     3781 agcgttcctc gctctggcgc gtgcccgccg cggtgctgag caggttgcgg tagcgcgccg
     3841 cgtcggtacg ggcgaaggac agcgcggccg atttctccgc gacttcggcg ccggcctggt
     3901 cgatcagggt ctgctgcagc gtgcggcgcg ccagcagttg cgcgcgcgag gcgcgcatgg
     3961 ccgcctcgtt ggccttggcg gcatccagcg cggcggcgaa atcgccgggc tgcaggcgca
     4021 gcagcggctg gccggcgcgc acgcgctggt tgtcctccac cagcagctca gacacgtagc
     4081 ccgccacgtg cggcgagatc accgtgatgt tgccgcccac gtaggcgtcg tcggtggtct
     4141 cgtagaagcg cccgtgcagc caccagtaga tcgcgtagcc ggcgagcagc accagcagca
     4201 cgatcagcac cggcagcacc agcttgcgct tgcgtttcgg ttttccgtcg gcggcgcccg
     4261 gcagtgccgt cgcgcccgtt tggttctcgc tcaagttgag gttctccttc gcctggcttg
     4321 cttggccgtc ctcgccggga tcatgcgttg catgcgcaac gatcgcggcg attcatgccg
     4381 gtttcagatg gtcatcgtca tatgaagagg ccgaattccg gcatgcccga acgccgggca
     4441 tgccggagcc tggcgcggcg cgatggcccg cgcggccctg ccttccgtac agcaatcacg
     4501 gcgcctcgct cacgcggcgc gccgcgtgtc ctaccaggtg ccgagcagcg aatcgacgcc
     4561 gcgcagcgcg ttgttgcggc gatagggcag cgcgtcgaga ttggtcaggt gcaggttcgg
     4621 caggcgcgtg tagaccacct ccagcgcggc cgcgatctcc agcagcgcga gccgcgcgcc
     4681 caggcagtag tgcaggccgc cgccgaacga gagcgggcgc acgttctcgc ggtcgatgtc
     4741 gagccggtcc gggttctcga acttctccgg atcgcgattg ccgccgccga ggaacacgca
     4801 gatcaggtcg ccgcgctgga tcgtctggcc ttccagctcg acttcctcga gcgccacgcg
     4861 ctgggcgatc tgcaccgagc tgtcgtagcg cagcgcctcg gtcaccaccc ggggcatgat
     4921 cgagaggtcg gccttgacgc gctcgcgctg ctccgggtgg cgctgcagcg cgatcagcgt
     4981 gttgccgatc atgttctcgg tggtctcgtg gccggccacg aacaggaagc agaggttggc
     5041 gacgatctcg tcgtcggtca ggcgctggcc gtcttcctcg gccaggatca tctgcgagat
     5101 caggtcgtcg cccggctcct tgcggcgctc ggccaacacc ttggcgaagt aggtttcgag
     5161 ttcgatcgcc gacttgcgcg attcctcgag ctgctcggcg ttgagcggcg tgacgttgag
     5221 cgcgccggcc gcgcggtcgg cggcgcgctt gaacatcgcg ccgtccgaca gcggcacgcc
     5281 gaggatggtg cacatggtct gcatcggcag caggaaggcg aagccggtca ccaggtcgcc
     5341 ctgcggctcc ttcaccagcc ggtcgatcaa ctggttcgag atggtgtagg aggcctcgcg
     5401 gaacttctcg acctggcgcg cgttgaagga cttcatcagc agcgcgcgca ggttggtgtg
     5461 cttgggcggg ttcaggcccg gcagcatcga gcggaaggtg cggaacggcg gctggtcgat
     5521 cacgccctcg ccgtagcgcg ccttgaccgc ctcgacgatg cccttgccga agcggcggtc
     5581 gagcagcagc ttctcgccga agccgtagtg cgagctcgcg tagagcttgg gcgcgagctt
     5641 gaccagttgg ccctcggcgc gcatcgcgtc gaacacgggg taggggttct tcaggaaatc
     5701 gggtgtcgcc aggtcgctgt attgcatggt ggggagtcct tggtgtcttg ggaagtggcg
     5761 ctcgggcagc cgcgcctcag atcgagacca tctcgaagtc gagcttgggc acgtcgcatt
     5821 cggggcagcg ccagtcgtcg ggaatgtcct cccagcgcgt gccggcggcg atcccggcgt
     5881 tcgggtcgcc gcttcgttcg tcatagaggt aaccgcagat caggcacatc caggtgcggt
     5941 attcgacggt gagttcggac atcgtgagcg ttctccggat cagtgctgca gggcaggctc
     6001 ggcggcgacc gcgctcgcgc cttccaggtt ctggtgcagc tgcttgcgca gcttgtggag
     6061 gtccttgacg ttgagcagcg gacggtccag caggatcgac aggttgtgca ggatgcggtc
     6121 ggccacgcgg ttctcccagt cgcggtcgaa gcggatctgc gtgtcgagcc agcggcccag
     6181 ccattcggga tcgggcaggc ggctctgcac cgtgtcgttg gggaacagcg tgcgattcac
     6241 gtgcaggttg gtcgggtgga tcgccttggc cgcgcggttg gcgctggcca tcagcgtgcc
     6301 gatcttggcg aaggccaggc gcgcggcgtc gccgtgccgg tccagcgcgc ggcgcatgta
     6361 cttcaggtag gcggccgcgt ggcgcgcctc gtcgcgcgag atcagctcgt agatctgccg
     6421 gatcaccggc tcggtgtgcc agtgcgcggc gcagcgatac cagtggttga gacggatctc
     6481 gccgccgaag tgcagggtca gcgtctccag cgccggggcc gggtcgaagg ggaagcgcac
     6541 cgcgtgcagc tcgtcctcgg tgggcgcgag ctcgggacgg aagcgccgca ggtactcgat
     6601 cagcaccagc gagtgcttct gctcctcgta gaaccagatc gacatgaagg ccgagaaatc
     6661 gctgtcgtcg acgttgtcgc gcaggaacat ctcggtggcc ggcagcgccg cccattccgt
     6721 gatcgcgttc atcttgatcg acagcgcctg ctcgtcggac agcagcgagg cgtcgaagcg
     6781 gtcccagtcg acgtcgtgct gcatgtccca gcgcgcgcgt tccaggcttc ggtacagttc
     6841 gggatagagc atggtggtcc cctcggctca gcctgtgacc ggcgccagcg acggcgcgtt
     6901 cgccgccggc tcgccgccga tcgcgaggaa ctgggtggcg cgctcgatca cgcgcgcgtc
     6961 cgacaggatc cgcacgtggc cgaccttctc gacgcgctcg aagcgcacgt gcggcaaggc
     7021 cgcggcgatg ttctccgatt cgcagatcgg cacgatcttg tcgttcgggc catgcatcac
     7081 gaagatccgc gtgcgcaggc cccggcccag cgcgccgatg tcccagtgct ggggcggcac
     7141 gccgttgcgg cgatgcagct cggcatacat cgccgccatc acgctgggcg cgaggcgcag
     7201 gaagtcgccc gaccagcggt cgatcaccgc cggcagcgtg cagggcggcg ccagcatcac
     7261 gatgccgtcc acgctgtcgg gcccgagctc ggccatcgcg ccggtcgagg cgatgctgcc
     7321 cagcgaatgc gagacgatgc cgtgcacgcc gccgagcgat tcgatcgcgc ccttcacggc
     7381 gttcttgaag gccgtcatgg tggtgaccgt gccgggcgag acgccgtgcg ccggcgcgtc
     7441 gaaggcggcc acgcgaaaac ccgccttctg cagcttgggc acgaaggaga acatggtggc
     7501 gctgtcggcg ccccagccgt gcaccagcag cacggtgcgc tcgcccttgc cccacagatg
     7561 gccgtgggtg acgccgtcca cgccgcggat ctcgaagcgg cgcgcgccga gcggcgacat
     7621 gtccttgggc ggcgtcttgc ggaaggtgcg cgtatagccg aacaggtcgg tggcgcgccg
     7681 cgcggcggcc gccggcgaga ctgcgccgag cgcgccgacg tagcccttga gcacgcgctt
     7741 gccgaacgac atcgcggggc tcgccgcgcg tgccggcgaa ggcgccggcg cggcggcggc
     7801 cgccgcgccg tgcgggaggg atgactcgga agtattggac atcgtccttc tcctgccagg
     7861 gtggcctgat gaagccgggt agttggctca ggtagtgggc tcagatcgcc gaatcggcgc
     7921 gcggcggggt ggccgcgggc ggcgtggcga gcgcctcgaa cgagcggtag tagccgtgca
     7981 ccgggtcata cagatggcag gcgcggatca ccttccagac ctcgccgagc gtgaacggct
     8041 tctgccggat cgagccggga taggcgcgct tgagtgcctc gcgtgccttg ggcaggttgt
     8101 agaaggggat caccggcacc acgtggtggg cggcatgcac cgagatgttg tgggtgaaga
     8161 agtgcagcca cttcgggtac tcgtagtcgg tggtcatcat caggcggctg ccgttcaggc
     8221 tccagtgctc gcgcgtcagg aacggcagtt cgtccgatat gtgatgcatc atcgtggtgg
     8281 tgctgaacca ggcgtggatg ccgagccagg ggccgaggaa gtacagcagg aaaccctgcc
     8341 agcccgtgaa ccagaccagg atgggcaggt agacggcggc gaacagcacc gtgaccgcca
     8401 ccgagcggcg cacgtcgttg cgggcctcgc gcttggggaa catgctgggc ttgaagcccg
     8461 agtggcgctg gtagttgacc gtgcccagcc agaacagcca gctgcgcgtg ctgcggtaca
     8521 cgaacttctc ccaggcgccc atgcggcggt attgctccgg cagcaccggg cgccagtcgg
     8581 tatccatctc caggctgttg gtgttggcgt gatgcaggtt gtgcatgtgg cgccagccgt
     8641 ggaacgggta gagcagcggg aacagcgcga tctggccgag cgcgaagttg aagcggcggc
     8701 tcttcgagaa ggagttgtgc ccgcaatcgt gggcgatgca gaacaggccc cagccgccca
     8761 ggccggccag cagccagagt gggatccaga gcgaccagtg cggcgcgtag tgcacgccga
     8821 tcacggcgcc cacgtagagc gcggtgctgg cgaagaagcc cagcaggccg cgcgccaggc
     8881 gcggttcgta gagttcgcgc ggaatcgcgt cggccaggcg ctcgccgtcg agcgacttga
     8941 tcgactggac gaagtcccga aagcccgggg tctgctgctg cggtagttgg atggttgcca
     9001 tcggttcgct ccttggtggg cctcaggcct tgatctcgcc ggtggcgagt tcggcggcga
     9061 gatagctcgc cagatcgtcg atgttctggt gttcgaacag cagcgccggc gacaggcgct
     9121 gctcgagcag cttctcgaga tcgcccgcga cctgcagcgc gaccagcgag tcgagcccgt
     9181 aggactcgaa gctgcgcgcc ggatcgatct gcgcgatcgg cacgttgagt tgcctggcca
     9241 gccgctcgac cagccattga tggatgcccg cctcggtgag cggggtcgag gaacgttttt
     9301 cctcgatgaa gccgatcttg ctgaacaggc tcgacatgaa cgccatgatg cgtactcctc
     9361 agggttgaag aaggcaaagc aaaacgaaac gatcgggatg cggcgcgctc aggcgccgcc
     9421 gggctcgcgc ttgcgcacgc gctcgatcac gtcgcggctg ggcgagcgca gctcctcgac
     9481 gatgccgagc cgtccgaaca gcgccagcat ccagcccgac aggtccggct cccaccagtg
     9541 aacgccgtgc cggtaccagg ccgggaaggc gtgatggttg ttctgcaggc cctcgccgaa
     9601 ggtcaggaag gccacgatcc agttgttggt gctgtggtcg cgcgtgtcga acgggcggct
     9661 gccgtagcga tggcagatcg agccgacgca ccaggcgaac tggttggcga tgaacatgcg
     9721 cgcgaagccg ccgaacatca ggccgcacag cgcgcccatc cagctcgcgg tgatcagccc
     9781 gccggccacg gccgggatcg cgaggccgag cagcacccag tagaagtagg tgcgattgaa
     9841 gaacagcagg ctgcggtcct tcaggatgtc ctgcgcgaag tagctccagc tcgacatctc
     9901 gggcgacatc atccagggca tgtgggcgtg ccacagcccg cgcaggcgct cggcgcggct
     9961 ctcgcccagc aggttgggcg aatgcgggtc gcccgggcgg tcgctgtagg cgtgatggcg
    10021 ccggtgcgta gtgacccaga acaggatcgg gccctgcgcg gccatcgagc cgcagatcag
    10081 gatcagcgcg cggaacagcg gcgaggtcct gaaggctcga tgggccagga agcggtggta
    10141 gccgatcgag acgccggcca tgtgcagcac gtacatcgcg gcgaacagcc aggcgtcgac
    10201 gcgcgtggcg tagccggccc aggccagcca ggccgccgcc agcgtgccgg ccaacggaat
    10261 cgtcatcacg ccgaaggcgg tcatcgcctt gatgcgggcg ccgcgcgcgt cgagcacgct
    10321 gacgccgcgc accggctggt cgtcggggag ggtcggcgcg ggggtgagat cgagctgggt
    10381 catgaaggaa tctccggaag gcgggcgctc aggactgggt ggcgcggctg cgctcggggg
    10441 ccgggcggcg cacgtcccac accaggccga acaccgccag cagctcgatc aggccgcccg
    10501 acaggtcgag ctgccagaag cgctggccgg tgcgtgcgag agccgggaag gcgtgatggt
    10561 tgttgtgcca gccgccgccc accgccggca gggccagcca gaacacgttg cggctctggt
    10621 cgcgggtgcg gttcgggcgc gggccgaagc tgtgcgcgaa ggagttgatc gcccaggtgg
    10681 cttggtcgac caggaagatg cgcaacaggc cgccccacag gaaacccgtc agggccgcgt
    10741 agccggtgcc gccgatcagc cagccggcca gcgtcggcag cgccaggccg agcgcgatcc
    10801 acaggaagta gagacggttc aggcgcacca cccagcggtc gcgcagcagg tcgggcgtga
    10861 actggctcca gttctgccgg cgcagcgtga acagccagcc gacgtgcgcg tgccagatgc
    10921 cgcgcaggcg cccgccgaga tgctcgccgt gcaggcgcgg cgaatgcggg tcgccttcct
    10981 ggtcggtgaa ggcgtggtgc atgcggtgcg tggcggccca gaacaggatc ggcccctgcg
    11041 cggccatcga gccaaagatc gcgaacagcg ccgtcaccac cgggccggcc ttgaaggcct
    11101 ggtgcgagaa gaagcgatgc agccccgcct cgacgccgag cgcggtgacc aggtacatca
    11161 ccgcgagcag gccgatctcg agccagccga tgccatgcac gatcgccagc gccacggccg
    11221 cgagcgcgcc gaccgccggg atcccgaccg tgagccaggc caggcgccgt tcggttgcgc
    11281 cggtgcgatc ggcgagcggc gcgggccgcg agggggaggt ttgggtctgg atgtccatga
    11341 tgtccttcgc gggctcaggc gcgtgcgtgg ggatagctcg ccagcgattg cgtcagcagc
    11401 gcgtggcggg tggcgtagcg ctggatcttg ccgctcgtgg tcagggggat cgcgcccatc
    11461 ggcgcgaaat ggatcaggtg cggcgcggcg ccgagctgct gcgtgacacg ctcgacgatt
    11521 gcctcgcgca gcgtctgcag ctcgccctcg gccaccttct cgccgcgccg gagttcggcc
    11581 accacggcga tccgctcgcc ctggccgtcg tcgatcgaga aggcggccac gccgttcggg
    11641 cggatccgcg cgtcgagctc ctccaccagc agttcgatat cctgaggata gagattgcgg
    11701 cccgcgtgga tgatcaggtc cttgatgcgg ccggtgacgt acagctcgcc cgcgcgcagg
    11761 aaaccgaggt cgccggtgcg catgtagcgt tgtgccgagc cttccaggcg cgcgtagaag
    11821 gtctcctcgc tgcgctcggg ctggttccag tagccgatgc cgatgttcgg gccgcccacc
    11881 cagatttcgc cgatcgcgcc gtcgggcagc gcgcggccgt ccgggccgac gatccgcacg
    11941 tcgtggcccg gcgccggcgc gccgcaggag gccagcgcct gggcgcgcgg atgatcggcc
    12001 gcgatctcgc gcacctcgcc gcgcgccagg ccgtcctggt cgagctcgac gaaggcgggc
    12061 gcggcgccgt ccaccttgcc cgatacgaac acggtggcct cggccagtcc gtagcagggg
    12121 ccgaaggcct cggcgcggaa gccgcagcgc gcgaagcgct cggcgaaacg ctcgaaggtg
    12181 cggcgccgga ccggctcggc gccgcagtag atcgcctcga ccgtggacag gtcgagcagc
    12241 ggcaatacat cgtcgccgat cgcgttgcaa cagaggtcga aggcgaagtt cggcgcgatg
    12301 gtggtggtga cgcggtactg cgagagcgcc gccagccagc gcagcggctg ctggacgaag
    12361 tggccgggcg agaggatcac cgtcgggaaa ccctcgtaga tcggctgcag gatgccgccc
    12421 atcaggccca tgtcgtgata gggcggcaac caggtgcaac cgacgcgctc gcgcggcccg
    12481 cccatccagc ggctcgcgcc gtggcagttg ctgagcaggt tgccgtgggt cagcatcacg
    12541 cccttcggat cggaggtcga gcccgacgtg tactggagca gggcaagcgc ctcgcgatcg
    12601 agcaccggca aggccaggcc ggcatcggcc gggttgtatg catatgcatg agtatcgacg
    12661 cgaaaaagcg cgtcggcctc ggtccaggcc tcctccggga agccgtccgg cagcagcgcc
    12721 agcacgcgct cgcgcagccg cgtgaagcgc gaatcggtca ggatcagctc gggctgcgag
    12781 tcgcgcgcga tcgcgccgag ccgccccagc gcccgcgtgc ccaccggcgg gaacgagggc
    12841 accgccaccg cgccggccat ccagacgccg aacagcgcgg cgatgtagtc gatgccggcc
    12901 tcgaacagca tcagcacgcg cttgccggcc acgccgcgcg cggcgaggtg gcgcgcgatc
    12961 gtgcccgcct gcagctgcag ctcggcatag ctcacggtgc gggtctcgcc ttcgccgtcc
    13021 accaggtgga tgaaggcggt ctgcccgggc agcgtcgcgg cgcgcaggcg cagcacgtcg
    13081 gccagcgtgt cgagggcgtg ctcggccagc gtcgcggcgg cgcgatggcg cgaatggctc
    13141 gcggcgagcg catcgagcgc cgggcttgcg acttcgagat gtgtcat
//
