LOCUS       BGC0000895             11180 bp    DNA     linear   BCT 26-JUL-2016
DEFINITION  Lactobacillus plantarum subsp. plantarum citrulline biosynthetic
            gene cluster (carAB operon and argC, J, B, D, F-ccl operon) and usg
            and dsg partial sequences.
ACCESSION   BGC0000895
VERSION     BGC0000895
KEYWORDS    .
SOURCE      Lactiplantibacillus plantarum subsp. plantarum
  ORGANISM  Lactiplantibacillus plantarum subsp. plantarum
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
            Lactiplantibacillus.
COMMENT     On Oct 1, 1999 this sequence version replaced X99978.1.
            ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-07 20:59:03
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 11180
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     gene            <1..459
                     /gene="usg"
     source          1..11180
                     /db_xref="taxon:337330"
                     /mol_type="genomic DNA"
                     /organism="Lactiplantibacillus plantarum subsp. plantarum"
                     /strain="CCM 1904 equivalent to ATCC 8014"
                     /sub_species="plantarum"
     subregion       1..11180
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000895"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..11180
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     CDS             <1..377
                     /codon_start=3
                     /db_xref="UniProtKB/TrEMBL:Q9RLT0"
                     /gene="usg"
                     /note="upstream gene of citrulline biosynthetic cluster"
                     /product="hypothetical protein"
                     /protein_id="CAB56842.1"
                     /transl_table=11
                     /translation="ATDYLVTVKLYLGFRVRQDINRYLRTIVRDLMATGRLAAQKQTYS
                     VTSGRDVGDFRFVIIEEKLENGSRLSRLDRLVIETKLMIKKYATTPAKWFGLEFSEVTL
                     ETVPILFNEIPALPITERQR"
     regulatory      435..459
                     /gene="usg"
                     /inference="non-experimental evidence, no additional
                     details recorded"
                     /note="rho-independent"
                     /regulatory_class="terminator"
     regulatory      complement(435..459)
                     /gene="carB"
                     /note="rho-independent terminator of carAB operon"
                     /regulatory_class="terminator"
     gene            complement(order(435..459,484..3546))
                     /gene="carB"
     CDS             complement(484..3546)
                     /EC_number="6.3.5.5"
                     /codon_start=1
                     /db_xref="GOA:Q9RLS9"
                     /db_xref="InterPro:IPR005479"
                     /db_xref="InterPro:IPR005480"
                     /db_xref="InterPro:IPR005481"
                     /db_xref="InterPro:IPR005483"
                     /db_xref="InterPro:IPR006275"
                     /db_xref="InterPro:IPR011761"
                     /db_xref="InterPro:IPR013815"
                     /db_xref="InterPro:IPR013816"
                     /db_xref="InterPro:IPR013817"
                     /db_xref="InterPro:IPR016185"
                     /db_xref="UniProtKB/Swiss-Prot:Q9RLS9"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /gene="carB"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1090:acetyl-CoA carboxylase biotin carboxylase (Score:
                     64.2; E-value: 1.5e-19)"
                     /gene_kind="biosynthetic-additional"
                     /note="arginine specific"
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="CAB56843.1"
                     /transl_table=11
                     /translation="MPKNNAIHSIAVIGSGPIKIGQAAEFDYAGTQACLSLKAAGYHVI
                     LINSNPATIMTDTATADEVYLEPLTLTSVTKILRATHPDALLPTLGGQTGLNLAMALDQ
                     AGVLNELKIQLLGTSLATINQAEDRAAFKTLMKRLHQPIPASTTVHHVTSALSFAEKIG
                     YPVIVRPAFTLGGSGGGIANNAAELTQTLQRGLTMSPVTECLIEQSIAGFKEIEFEVMR
                     DNQGTKIIVCSMENFDPVGIHTGDSIVYAPVQTLTDTEYQQLRTAALTIVEALDIRGGC
                     NVQLAQDPNSRQYYVIEVNPRVSRSSALASKATGYPIAKIAADIAIGLNLSEIKNPVTQ
                     TTYAAFEPALDYVVAKIPRFAFDKFPTADAHLGTQMKATGEVMAIGSTLEEATLKAIAS
                     LEIDPKTQASLTPDHHVTTTEYIDQLTHPTDQRLFYLLAALQAGWPLAKLATLTQITPF
                     FLSKLQHIAQLIRNIKQVPTSQHLLSAKKYGVSLATMAHYAQTSVATIAAMTADLPFVY
                     KMVDTCAGEFASVTPYFYSTAFGQTNESHPLGHSILVLGSGPIRIGQGIEFDYTTVHCV
                     KAIQQAGYHAIIVNNNPETVSTDFSTSDKLYFEPLTIERLMPIIALEQPAGVIVQFGGQ
                     TAINLAHQLTKLGVTVLGTSVSATDLTEDRQSFADCLRLLKIAQAAGTTVTELSGARQA
                     AHAIGYPLLVRPSFVLGGRAMAIVHNDHELTPVIKSAVAAGHGAPILMDQYLAGTECEV
                     DVLSDGTSCFIPGIMEHIEGAGVHSGDSITVYPPQHLSPAVQDKIVTIATKLAQHLHCV
                     GMMNIQFVVTDDVYVIDVNPRASRTVPYMSKVTHLPLAQLATRLILGQSLATLGLVPGL
                     LTPAPQQIAIKAPVFSFNKLPQSPVVLSPEMKSTGETLGIGPTFTTAWHAAMADSYHLE
                     TWQTVDGLITDIATVAQPAVQELFAANHLTVTTIANTTDWPAKTKAVGFTLNDQPDNPV
                     AIAALNHGQPLITAIDTLKTLLAVTPTPATI"
     CDS             complement(3539..4606)
                     /EC_number="6.3.5.5"
                     /codon_start=1
                     /db_xref="GOA:O08317"
                     /db_xref="InterPro:IPR000991"
                     /db_xref="InterPro:IPR001317"
                     /db_xref="InterPro:IPR002474"
                     /db_xref="InterPro:IPR006220"
                     /db_xref="InterPro:IPR006274"
                     /db_xref="InterPro:IPR011702"
                     /db_xref="InterPro:IPR017926"
                     /db_xref="UniProtKB/Swiss-Prot:O08317"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /gene="carA"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1249:carbamoyl phosphate synthase small subunit
                     (Score: 266.9; E-value: 3.9e-81)"
                     /gene_kind="biosynthetic-additional"
                     /note="arginine-specific"
                     /product="carbamoyl-phosphate synthase small subunit"
                     /protein_id="CAA68238.2"
                     /transl_table=11
                     /translation="MNKYLTLADGTQWIGTAIGDCQLEVAGRIVFNTGMTGYQETLTDP
                     SYLNQMIAFTYPLIGNYGIDPTVAQAPTIGAQAIIVHELTTFNDHYTSRQSLASFLTIH
                     HVAGIEGVDTRDLTIHIRQTGAQMAILSNHPITDFEAQLATFAPQVLTATPLPVATTTI
                     RPRVAILNFGEKAAITAELQARGADVVVLPPTASLKAVAAYHPDGILLSNGPGDPTDYH
                     TYLATIRQLAQRYPLAGICLGHQLIALAYGAQTYQLSFGHHGLNHPVQACADGRIIMTS
                     QNHDYAVDPASIKGTPLIVTHTELNDGSIEGLRLPHQAVMSVQFHPEAHPGPQEAGQFF
                     DDFLLTIQKEAVVNA"
     gene            complement(order(3539..4606,4662..4690))
                     /gene="carA"
     misc_feature    complement(4590..4607)
                     /inference="non-experimental evidence, no additional
                     details recorded"
                     /note="Escherichia coli like Arg box termed R11"
     misc_feature    complement(4639..4656)
                     /inference="non-experimental evidence, no additional
                     details recorded"
                     /note="Bacillus subtilis like Arg box"
     regulatory      complement(4662..4690)
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /gene="carA"
                     /note="promoter of arginine-repressed carAB operon"
                     /regulatory_class="promoter"
     misc_feature    4679..4696
                     /inference="non-experimental evidence, no additional
                     details recorded"
                     /note="Escherichia coli like Arg box termed R1"
     misc_feature    complement(4679..4696)
                     /inference="non-experimental evidence, no additional
                     details recorded"
                     /note="Escherichia coli like Arg box termed R10"
     misc_feature    4719..4736
                     /inference="non-experimental evidence, no additional
                     details recorded"
                     /note="Escherichia coli like Arg box termed R2"
     misc_feature    4757..4774
                     /inference="non-experimental evidence, no additional
                     details recorded"
                     /note="Escherichia coli like Arg box termed R3"
     misc_feature    complement(4757..4774)
                     /inference="non-experimental evidence, no additional
                     details recorded"
                     /note="Escherichia coli like Arg box termed R9 and Bacillus
                     subtilis like arg box"
     misc_feature    4768..4785
                     /inference="non-experimental evidence, no additional
                     details recorded"
                     /note="Escherichia coli like Arg box termed R4"
     regulatory      4780..4808
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /gene="argC"
                     /note="promoter of arginine-repressed argCJBDF-ccl operon"
                     /regulatory_class="promoter"
     gene            4780..5899
                     /gene="argC"
     misc_feature    4797..4814
                     /gene="argC"
                     /inference="non-experimental evidence, no additional
                     details recorded"
                     /note="Escherichia coli like Arg box termed R5"
     misc_feature    complement(4808..4829)
                     /inference="non-experimental evidence, no additional
                     details recorded"
                     /note="Escherichia coli like Arg box termed R8"
     misc_feature    4825..4848
                     /gene="argC"
                     /inference="non-experimental evidence, no additional
                     details recorded"
                     /note="Escherichia coli like Arg box termed R6"
     misc_feature    4852..4869
                     /gene="argC"
                     /inference="non-experimental evidence, no additional
                     details recorded"
                     /note="Escherichia coli like Arg box termed R7"
     CDS             4874..5899
                     /EC_number="1.2.1.38"
                     /codon_start=1
                     /db_xref="GOA:O08318"
                     /db_xref="InterPro:IPR000534"
                     /db_xref="InterPro:IPR000706"
                     /db_xref="InterPro:IPR012280"
                     /db_xref="InterPro:IPR016040"
                     /db_xref="InterPro:IPR023013"
                     /db_xref="UniProtKB/Swiss-Prot:O08318"
                     /gene="argC"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1219:N-acetyl-gamma-glutamyl-phosphate reductase
                     (Score: 306.6; E-value: 3.5e-93)"
                     /gene_kind="biosynthetic-additional"
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="CAA68239.1"
                     /transl_table=11
                     /translation="MQVALVGVTGYSGMVLYRLLKQHPEIDRIQLYGHVGATTVALKTV
                     AAMYQKETAVIRPFDATAIMRDNAIVFFATSAGITRQLALPFIAAHFPVIDLSGDFRLH
                     DPEQYQRWYQRDPAPATALAQASYGLADMPAPLSTYIANPGCYATATLLGLAPLAQQQL
                     VEPTSIVVDAKSGLSGAGKRATAASHYVAVNDNLSLYKLNQHQHIPEMMQQLQQWWSAI
                     SAIEFTTTLIPATRGIMTTIYAKAKSALTTTQVRAAFTATYHEQPFVQVLPTGMPTLKD
                     VVGSNNCALGVDYNPVTNTIVVVSVIDNLMKGAAGQAVQNFNRYFDFAVTAGLPTLPVF
                     P"
     gene            5924..7138
                     /gene="argJ"
     CDS             5924..7138
                     /EC_number="2.3.1.35"
                     /codon_start=1
                     /db_xref="GOA:O08319"
                     /db_xref="InterPro:IPR002813"
                     /db_xref="InterPro:IPR016117"
                     /db_xref="UniProtKB/Swiss-Prot:O08319"
                     /gene="argJ"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1220:bifunctional ornithine (Score: 432.8; E-value:
                     1.5e-131)"
                     /gene_kind="biosynthetic-additional"
                     /product="glutamate N-acetyltransferase"
                     /protein_id="CAA68240.1"
                     /transl_table=11
                     /translation="MQMQVLTNTKFETVAFQWPAGFYSDGIHIGLRRHKKDFGWLFSKV
                     PASAAGTYTTNQFQAAPTKLTKQMIDQGHQLQGLLLNSAIANSCTGEQGWQNALQEQAW
                     LANKLNVAPNLIGVASTGLIGAQLPMDKIKNGLPQLTPTKSDAVTYAVLTTDQHPKTVC
                     VQCELSGQLVTLTGFAKGSGMIHPKMATMLGFVTTDAQIDGPVLQDMLSTNVDQTFNQI
                     TVDGDTSTNDMVVTLANGLADNPSLQAGTTDYDIFNQALHYVLGQLAKQIAADGEGATK
                     LVECNVVHAATTFDGQQIAKAIVGSNLVKAAIFGEDPNWGRIISTIGATDADIDVATVD
                     IEMNGVLLVQQSLAVDFDMAAVQATLRDNQIMIDVDLHHGTASGQAWGCDLTYNYVKIN
                     ASYHT"
     gene            7154..7900
                     /gene="argB"
     CDS             7154..7900
                     /EC_number="2.7.2.8"
                     /codon_start=1
                     /db_xref="GOA:O08320"
                     /db_xref="InterPro:IPR001048"
                     /db_xref="InterPro:IPR004662"
                     /db_xref="UniProtKB/Swiss-Prot:O08320"
                     /gene="argB"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1174:acetylglutamate kinase (Score: 169.7; E-value:
                     1.4e-51)"
                     /gene_kind="biosynthetic-additional"
                     /product="acetylglutamate kinase"
                     /protein_id="CAA68241.1"
                     /transl_table=11
                     /translation="MTKLIVIKIGGQAISQLSTTFFDQIAQWYQQQYQILIVHGGGPMI
                     NRLTTQLALPVHKVNGLRVTDAATLVLTKLALLGDAQPALLAKLTQHHLPVLGLNAADN
                     QLLTGELIDYRQLGYVGRLTAVNQVQLMQLLAHHIGILAPLALTETGQWLNVNADMAAT
                     VLAQQLHAEKLVLLTDVPGIIHHGNVMTSLSPQQAQQLIRTAVITAGMQPKVQAAIAAI
                     QTGVKQAIITNAIDQPGTAIIQEVAV"
     gene            7897..9066
                     /gene="argD"
     CDS             7897..9066
                     /EC_number="2.6.1.11"
                     /NRPS_PKS="Domain: Aminotran_3 (16-327). E-value: 1.7e-90.
                     Score: 295.3. Matches aSDomain:
                     nrpspksdomains_argD_Aminotran_3.1"
                     /codon_start=1
                     /db_xref="GOA:O08321"
                     /db_xref="InterPro:IPR004636"
                     /db_xref="InterPro:IPR005814"
                     /db_xref="InterPro:IPR015421"
                     /db_xref="InterPro:IPR015422"
                     /db_xref="InterPro:IPR015424"
                     /db_xref="UniProtKB/Swiss-Prot:O08321"
                     /gene="argD"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013:aminotransferase class-III (Score: 365.3;
                     E-value: 6.6e-111)"
                     /gene_kind="biosynthetic-additional"
                     /product="acetylornithine aminotransferase"
                     /protein_id="CAA68242.1"
                     /transl_table=11
                     /translation="MTMQHVFPTYQRFPFAITDGQGVHLTDNHGKTYLDFTAGIGVCNF
                     GYHQPQIQAAVTQQLTHIWHTSNLYENELQDAVAGLLANGEERLVYFANSGTEANEAAL
                     KLARKYTGKTGILAFQHSFHGRTYGAMSMTGNPHIQAGYAPLVPGITFATYNDDAALDK
                     ITPELAAVILEVVQGEGGVFAGQTAWLQAVNAKCQATGVLLIIDEVQTGIGRTGYRMAY
                     EGYGLDPDIYTVAKGLANGLPVGAMVGRRQLATAFGPGSHGSTFAGNAVAMAAAKRVLT
                     QLTPALLTTVRAHAKLVWQSLATQVEPIPVVKQITGKGLMIGIHLDEQIPVNQVITRLQ
                     VEGLLTLSAGDNTLRLLPPIVMQPADLLAGIALIAKVLTTLTTEVTTNE"
     aSDomain        7945..8877
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_argD_Aminotran_3.1"
                     /evalue="1.70E-90"
                     /label="argD_Aminotran_3.1"
                     /locus_tag="argD"
                     /protein_end="327"
                     /protein_start="16"
                     /score="295.3"
                     /tool="antismash"
                     /translation="ITDGQGVHLTDNHGKTYLDFTAGIGVCNFGYHQPQIQAAVTQQLT
                     HIWHTSNLYENELQDAVAGLLANGEERLVYFANSGTEANEAALKLARKYTGKTGILAFQ
                     HSFHGRTYGAMSMTGNPHIQAGYAPLVPGITFATYNDDAALDKITPELAAVILEVVQGE
                     GGVFAGQTAWLQAVNAKCQATGVLLIIDEVQTGIGRTGYRMAYEGYGLDPDIYTVAKGL
                     ANGLPVGAMVGRRQLATAFGPGSHGSTFAGNAVAMAAAKRVLTQLTPALLTTVRAHAKL
                     VWQSLATQVEPIPVVKQITGKGLMIGIHLD"
     gene            9059..10081
                     /gene="argF"
     CDS             9059..10081
                     /EC_number="2.1.3.3"
                     /codon_start=1
                     /db_xref="GOA:O08322"
                     /db_xref="InterPro:IPR002292"
                     /db_xref="InterPro:IPR006130"
                     /db_xref="InterPro:IPR006131"
                     /db_xref="InterPro:IPR006132"
                     /db_xref="UniProtKB/Swiss-Prot:O08322"
                     /gene="argF"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1114:ornithine carbamoyltransferase (Score: 338.9;
                     E-value: 4e-103)"
                     /gene_kind="biosynthetic-additional"
                     /product="anabolic ornithine carbamoyltransferase"
                     /protein_id="CAA68243.1"
                     /transl_table=11
                     /translation="MNNQFEGRSFLKEIDYTPTELAYLIDFASHLKTLKAHHIPHPYLQ
                     GKNIALLFEKTSTRTRSAFTVAANDLGANPEFLGKDDIQFGTKESVVDTAKVLGSMYDG
                     IEFRGFKQSTVEDLATYSGVPVWNGLTDEWHPTQILADFLTLKEHFGHLKGLTLAYVGD
                     GRNNMANSLLVAGAMLGINIAIGAPVALQPAPAVVALAQQYAQAAGSTVVITADPQQAV
                     TQADALYTDVWVSMGEQVDYGERIKQLLPFQINTTLLAATGKSSTIVMHCLPALHDLKT
                     QLGAKLGEQYGMTAFEITDDVFQSKQAVVFEEAGNRMPAIKAVMAATLGNLFIPTSIFN
                     "
     regulatory      10087..10110
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /gene="ccl"
                     /regulatory_class="promoter"
     gene            10087..10769
                     /gene="ccl"
     CDS             10190..10696
                     /codon_start=1
                     /db_xref="InterPro:IPR010387"
                     /db_xref="UniProtKB/TrEMBL:O05109"
                     /function="not essential gene which encodes for a putative
                     hydrophobic protein"
                     /gene="ccl"
                     /note="citrulline cluster-linked gene"
                     /protein_id="CAA68244.1"
                     /transl_table=11
                     /translation="MTQSKIRPWIINALVAAMYVVLCLGPAAFSLASGAIQFRVSEGLN
                     HLAVFNRKYIWGIVAGVILFDAFGPGASLLNVLFGGGQSLLALLVLTWLAPKLKTVWQR
                     MLLNIALFTVSMFMIALMITMMSSGVAFWPTYLTTALSELIIMSITAPIMYSLDRVLHF
                     SDRIQ"
     CDS             complement(10297..10629)
                     /codon_start=1
                     /db_xref="UniProtKB/TrEMBL:Q9RLS8"
                     /note="unnamed protein product; function unknown and CDS
                     within ccl gene"
                     /protein_id="CAB56844.1"
                     /transl_table=11
                     /translation="MIINSDKAVVRYVGQKATPLLIMVIISAIINIETVNSAIFSNIRC
                     QTVFNLGANHVNTSRANNDWPPPKRTLRRLAPGPKASNKITPATIPQIYLRLNTAKWFN
                     PSETRN"
     regulatory      10727..10769
                     /gene="ccl"
                     /note="rho-independent terminator of argCJBDF-ccl operon
                     and ccl gene"
                     /regulatory_class="terminator"
     regulatory      complement(10945..10965)
                     /gene="dsg"
                     /inference="non-experimental evidence, no additional
                     details recorded"
                     /note="rho-independent"
                     /regulatory_class="terminator"
     gene            complement(10945..>11180)
                     /gene="dsg"
     CDS             complement(10974..>11180)
                     /codon_start=1
                     /db_xref="UniProtKB/TrEMBL:O05110"
                     /gene="dsg"
                     /note="provisonal name for downstream gene of citrulline
                     biosynthetic cluster"
                     /protein_id="CAA68245.1"
                     /transl_table=11
                     /translation="DLAAMAKIITKYTSIAGCLTLDVQNGTLGYRTLFTLPENVDSEKL
                     KRIIDNLNRKQFKVIEVEDLQAE"
ORIGIN
        1 tagcaacgga ctatttggta actgttaagt tatacctggg ctttcgtgtc cgccaagata
       61 ttaaccgcta tttacggacg attgtccgcg acttaatggc tactggacgt ttagcggcgc
      121 aaaagcagac atattcagtc acttctggcc gcgatgtcgg tgacttccgc ttcgttatca
      181 tcgaggagaa gttagaaaat ggtagtcggt tatcccgctt ggatcgatta gtgatcgaga
      241 cgaaattaat gattaaaaaa tacgcgacaa cgcctgccaa atggttcggc ctagaattct
      301 ccgaggtgac tcttgaaacc gttccaattc tattcaacga gatcccggcg ttaccgatca
      361 ctgaaagaca acgttaacga cattatcagt atttggagat ttcgctcaaa tgaaatggct
      421 aaggccaaaa taaagcacct atcgaaaatt gataggtgct ttattttagt gctgccaaac
      481 aagttagatg gtagctgggg ttggtgttac tgctaataat gtttttaagg tatcaatcgc
      541 cgtaatcaat ggttgtccat gattcaatgc agcgatagcc acgggattgt caggttggtc
      601 attaagcgtg aaacccactg ctttagtctt tgcaggccaa tcagtcgtgt tagcgattgt
      661 tgtgacggtg aggtggttag ccgcgaacag ttcctggacc gcgggttgcg ccacagtggc
      721 aatatcggtg atgagaccat ccacggtttg ccaagtctct aagtgataac tatcagccat
      781 ggcggcatgc caagcggtcg tgaaagtcgg gccgatacca agagtctcac cagttgattt
      841 catctcagga cttaaaacga ctggtgattg tggcaattta ttgaaagaga agactggggc
      901 ctttatagca atttgttgtg gagcgggtgt caagagtccg gggactaggc caagggttgc
      961 taaactttga ccgagaatta gccgggttgc caattgtgcc agtggcaagt gagtaacctt
     1021 gctcatgtaa ggaactgttc gacttgcccg cggattaacg tcgattacgt agacatcatc
     1081 ggtgacgacg aactgaatgt tcatcatacc gacacagtgg aggtgttgtg ctaatttggt
     1141 ggcaatggtg acgatcttat cttgaacagc agggctgagg tgctgtggtg gatagactgt
     1201 aatggaatcg ccagaatgaa cgccagcacc ttcaatatgt tccatgattc ctggaatgaa
     1261 gcacgacgtt ccgtcggaga gaacgtcaac ttcacactcg gttcccgcca agtattggtc
     1321 cattaagata ggcgcaccat ggccagctgc caccgctgat ttaataactg gcgtgagctc
     1381 atgatcgtta tgaacgattg ccatggcccg gccgccgagt acaaagcttg gccgtacgag
     1441 taaggggtaa ccgattgcgt gagctgcttg acgggcaccg gataactcag ttacggtcgt
     1501 tccagcggct tgggcaatct ttagtagacg taaacagtcc gcaaaagatt gccggtcttc
     1561 ggtcaaatcg gtagcggaca cactggttcc taggacggtg actcctaatt tagtgagctg
     1621 atgggcaagg ttgatcgcag tttggccccc aaattgaacg atgacgcccg cgggttgctc
     1681 caacgcaatg attggcatca aacgttcgat tgttagtggc tcgaaataaa gcttatccga
     1741 ggtagaaaag tcggtggaga ccgtctcggg attgttattg acgatgattg catggtagcc
     1801 agcttgttga atggccttga cacagtgaac ggtggtgtag tcgaattcga tgccttgtcc
     1861 gattcgaatg gggcctgatc cgagtactaa aatactgtgt cctagtgggt gactctcgtt
     1921 agtttgtcca aaagcagtgc tgtaaaaata tggtgtaaca ctggcaaatt caccagcaca
     1981 cgtatcgacc attttataaa cgaagggtaa atcagcagtc atggcagcaa tggtcgctac
     2041 cgaagtttgg gcataatgcg ccatggtcgc cagactcaca ccgtactttt tcgccgataa
     2101 gaggtgttgg gacgtcggca cttgcttgat attccggatg agttgggcga tgtgctgtaa
     2161 cttactcaag aaaaatggcg tgatttgcgt tagcgtcgct aattttgcta acggccaacc
     2221 cgcttgcagc gccgccaata gatagaagag ccgctgatct gtcgggtggg tcaattggtc
     2281 gatatattca gtcgtggtga cgtggtggtc aggggtcaag gacgcttggg tcttgggatc
     2341 gatttcaagc gaagcaatcg ccttgagcgt ggcttcctcc aaggttgacc caatcgccat
     2401 gacctccccc gtggctttca tctgtgttcc caagtgagcg tcggccgttg gaaacttgtc
     2461 aaacgcaaac cgggggattt tggcaaccac atagtctaaa gctggttcaa aggccgcgta
     2521 ggttgtctgc gtgactggat tcttgatctc agataaattt agaccaattg cgatatcggc
     2581 tgcaattttg gcaattggat atcccgtcgc tttagaagct agcgccgatg aacgactgac
     2641 ccgcgggttc acttcgatga cgtagtactg acgactattc gggtcttgag cgagttgaac
     2701 gttgcaccca cctctaatgt cgagggcttc cacaatcgtc aaagccgccg ttcgtagctg
     2761 ctgatattca gtgtcagtca aagtctgaac gggggcatac acgatagaat cgccagtgtg
     2821 aataccgacc ggatcgaagt tttccatgga acagacgatg atcttggtac cctgattatc
     2881 acgcatgact tcgaattcaa tttctttgaa gccggcgata ctttgttcaa tcaggcactc
     2941 ggtgacgggg gacatggtca gcccccgctg taacgtttgg gtcaattcgg cggcattatt
     3001 ggcaatgcca ccacctgaac ccccgagtgt aaaggctgga cgaacaatga ccggataacc
     3061 gattttttca gcaaacgaca atgcactggt cacgtggtga acagtggtac ttgctggaat
     3121 cggttgatgg agccgcttca taagtgtttt aaatgctgcc cggtcttcag cttgattgat
     3181 ggtggctagg gaagttccta gcagttggat cttcaactca ttaagaacgc cagcctgatc
     3241 taacgccatc gctaggttca atccggtttg tccaccaagt gtcgggagca aagcatcagg
     3301 gtgggtggcc cgcagaattt tagtgacact ggtcaaagtc agaggttcta agtagacttc
     3361 gtcggcggtg gccgtgtcag tcatgatggt tgctgggttt gaattgatca aaatgacatg
     3421 atagccggcc gctttaaggc ttagacaagc ctgagtaccg gcatagtcga actcagcggc
     3481 ttgaccgatt ttgattggac cagaaccgat gacagcaatc gaatgaatgg cattgttttt
     3541 aggcattaac aaccgcctcc ttttgaattg ttagtaagaa atcatcgaaa aattgcccag
     3601 cttcctgggg tcccgggtgt gcttcgggat ggaattgcac cgacattacg gcctggtgcg
     3661 gtaggcgcaa gccttcaatg ctgccgtcat ttaattcagt gtgcgtgacg atcagcgggg
     3721 tgcccttgat cgatgccgga tcgacggcgt aatcatgatt ttgcgaagtc atgataatac
     3781 gaccgtcagc gcaggcttgt accggatggt ttagcccgtg gtgaccaaag gaaagctggt
     3841 aagtctgagc accataggcg agcgcaatca actgatgacc aaggcaaatg ccagccagcg
     3901 gataacgttg tgcgagttgg cgaatggtcg ctaagtaagt gtgatagtcg gtcggatcac
     3961 cgggaccgtt ggataacaaa attccgtcag ggtgatacgc ggccacggcc ttgagactag
     4021 ctgtaggtgg aaggacaaca acgtccgcgc cccgggcttg caattcagcg gtgatagctg
     4081 ctttttcacc aaagttgaga atcgcgacgc gtggacggat ggtggtcgtc gctactggga
     4141 ggggggtagc cgttagtact tggggtgcga atgtggcgag ttgcgcttca aaatcagtga
     4201 tgggatgatt gctcaagatt gccatctggg ctcccgtttg gcgaatgtga atcgttaggt
     4261 cacgagtatc gacgccttca atgccggcta catggtggat tgtcagaaaa cttgccagtg
     4321 attggcgact agtatagtga tcattaaaag tggtcagctc gtgaacgatg atggcttgtg
     4381 cgccaatcgt tggtgcctgg gccacggtcg gatcgatacc ataattacca attaatgggt
     4441 aagtgaaggc aatcatttga tttaggtagg atggatcagt caatgtttcc tgataacccg
     4501 tcattcccgt attgaagacg atgcgccccg caacctctag ctggcaatcc ccaattgccg
     4561 ttccaatcca ctgtgtgccg tccgctaaag ttaagtattt gttcatccca atcgcacctt
     4621 tctgataatt tggttaaaat ttcattttgg ttaatgggat aagtaaacat cattttattg
     4681 tgaatgtcaa ttaaaaatac atattaagtg cataaataac tgaataaagc taaaaatatg
     4741 caattaatag aataaaagtg aatatttttt caataaagct tgcaatttaa aagcgatgag
     4801 aatatagtgt gaatcatact ttttgcatga acatgcagct aatacactat ttttgaatat
     4861 ttgaggtgat tatatgcaag tcgcgctagt tggcgttacg gggtacagtg gcatggtgtt
     4921 gtaccggctc ttgaaacaac atcccgagat cgatcgcatc caattatacg ggcacgtggg
     4981 agcaactacg gtggcattaa agacggtggc agccatgtac cagaaagaaa cggcggttat
     5041 tcgaccattc gatgcgacgg caatcatgcg cgataacgcg attgtgttct ttgcgacatc
     5101 agcgggtatc acgcgacaat tggcattacc attcatcgct gctcattttc cagtcatcga
     5161 tttatctggt gacttccgct tgcatgatcc agagcaatat cagcggtggt accaacgtga
     5221 tccagcaccg gcgaccgctt tggcacaggc gagctacggc ttagctgata tgccggcgcc
     5281 cttgtccacc tacattgcga atcccggctg ttatgcgact gctacgttat taggactagc
     5341 gccacttgct cagcagcagt tggtcgaacc gacttcgatt gttgtggatg ctaagtccgg
     5401 cttatctggt gctggcaaac gagcgacagc tgcgagtcat tacgtcgctg tgaatgataa
     5461 tttgagttta tataagttga atcaacatca acatatccct gaaatgatgc aacagttaca
     5521 gcagtggtgg tccgcgattt ctgcgattga atttactacc actttgattc cagcaacgcg
     5581 tgggattatg acgacgattt atgccaaagc gaagtcagca ttgacaacga cgcaggtccg
     5641 cgcagctttt accgcgacct atcatgagca gccgttcgtg caagtgctac caacggggat
     5701 gccgacgctc aaggacgttg ttgggtctaa taactgtgcg ttaggcgttg attataatcc
     5761 ggtcacgaac accatcgtgg ttgtgagcgt cattgacaat ttgatgaaag gtgctgcggg
     5821 gcaggccgtt cagaatttta atcgctattt tgatttcgcg gttacggccg gattaccgac
     5881 gttacctgta tttccctgat aaggtgcaat aagagaggaa gttatgcaga tgcaagtttt
     5941 aacgaatacg aagtttgaaa cggttgcctt tcagtggccg gcaggttttt acagtgatgg
     6001 cattcatatt ggtcttcgtc gtcataaaaa agattttggt tggctatttt caaaagtgcc
     6061 tgccagtgcg gctggaactt atacgacgaa tcagtttcag gcagcaccaa ctaagttaac
     6121 taaacagatg atcgaccagg gccaccaatt acagggactg ctgctgaaca gtgcaatcgc
     6181 caattcttgt actggcgagc aggggtggca gaatgcgctg caagaacaag cctggttagc
     6241 gaataaacta aacgtggcgc cgaacttgat tggcgttgct tcaactgggc tgattggtgc
     6301 tcagttaccc atggataaaa tcaaaaacgg cttgccgcaa ttgaccccaa cgaagagtga
     6361 cgcggtgacc tacgccgtgt taacgacaga ccagcatcca aaaaccgtct gtgtgcagtg
     6421 tgaactcagc ggccagttag ttaccttgac cggttttgcc aaaggttctg gaatgattca
     6481 tcccaagatg gcaaccatgc ttggttttgt caccactgat gcccaaatag atgggccagt
     6541 tttacaggac atgctaagta ctaacgttga tcagacattc aatcaaatta ctgttgacgg
     6601 tgacacgtct actaatgaca tggtggtcac gttggcaaat ggtctggcag ataatccgag
     6661 tttacaagcg ggtaccaccg actatgatat ttttaatcag gcgttgcact atgtcttagg
     6721 ccaacttgcc aagcaaattg ccgctgacgg tgagggtgcg accaagttag tcgaatgtaa
     6781 tgtggtccac gccgctacaa cgtttgatgg tcagcaaatc gccaaagcaa ttgttggctc
     6841 taacctcgtt aaggcggcca ttttcggtga agacccgaat tgggggcgaa tcattagcac
     6901 gattggcgcc actgatgcag acatcgatgt ggcgacggtc gatattgaaa tgaacggcgt
     6961 tttacttgtt caacaaagtc tggccgttga ctttgatatg gcagcggtgc aggcgacctt
     7021 acgtgataac cagattatga tcgatgttga tttacatcac ggtacagcaa gtggtcaggc
     7081 ttggggttgt gacttgactt acaactacgt caaaattaac gcgagctatc atacttaaac
     7141 ggaggtggtg aaaatgacaa aattgattgt aattaagatt gggggccagg cgattagcca
     7201 attgagcacc actttctttg accaaatcgc acagtggtac caacagcagt atcagattct
     7261 gatcgtacac ggtggggggc caatgatcaa tcgactgacg actcagcttg ctttgcccgt
     7321 gcataaggtg aacgggttgc gggtgacgga tgcggcgacc ttagtgctca ccaagttggc
     7381 cctgctagga gatgcgcaac cagccttact tgcaaaatta acgcaacatc acctacctgt
     7441 gttggggctc aatgcggcgg ataatcagtt actaactggt gaattgattg attatcgaca
     7501 gctagggtat gtcggccggc tcaccgctgt caatcaagtg caattaatgc aattgttggc
     7561 tcatcatatc ggtattttgg cccctttagc cttaactgag acgggacaat ggctcaatgt
     7621 caatgcggat atggcagcga ccgtgctagc tcaacaacta catgctgaaa aattggtttt
     7681 gctaaccgat gtcccgggaa ttatacatca cggtaatgtg atgacatcac tatcgccaca
     7741 acaagctcag caattgattc gaacagctgt gatcaccgcg ggaatgcaac ccaaagttca
     7801 agctgcaatt gccgcgattc agacgggagt caagcaggct attattacga atgcaatcga
     7861 tcaaccagga accgcaatta ttcaggaggt ggccgtatga ccatgcaaca cgtgtttcca
     7921 acctatcaac gttttccatt tgccattacg gatggtcagg gcgttcatct gactgataat
     7981 catggcaaga cgtatttaga ttttacggcg ggaatcgggg tctgcaactt tgggtatcac
     8041 cagccacaaa ttcaggcagc cgtcacccag cagttgacgc atatctggca tacgtcgaat
     8101 ttatacgaaa atgaattaca agatgccgtt gccggcctgt tagcgaatgg tgaagagcga
     8161 ctcgtatact ttgcaaattc cggaaccgaa gctaatgaag ctgcattaaa actcgcacgg
     8221 aagtacacgg gtaaaaccgg catcttagct tttcagcatt catttcacgg tcgaacctat
     8281 ggcgctatgt cgatgacggg caatcctcat attcaggctg gctatgcgcc attagtgcct
     8341 ggcatcacgt ttgcaaccta taatgacgac gcggctttgg acaaaatcac accagagttg
     8401 gcggccgtta ttttagaagt cgttcaaggt gaaggcggcg tctttgcggg acaaacagct
     8461 tggttacaag cggttaatgc gaaatgccaa gcaacgggtg ttttgttgat tattgatgag
     8521 gtgcagacgg gaatcggtcg gactggttac cggatggcct atgaaggtta cggcctggat
     8581 cctgatatct acacggtcgc caagggctta gcgaatggct tgccagtggg ggcaatggtt
     8641 ggtcggcggc aattagccac tgcctttggt ccgggcagcc atggctcgac gttcgccggt
     8701 aatgctgttg ctatggcggc ggcaaaacgt gtcttgaccc aattaacacc ggcgctgtta
     8761 acgacggttc gagcccatgc caaacttgtc tggcaatccc tagcgactca agtcgaaccg
     8821 ataccggtag tgaaacaaat cacggggaag ggcctaatga ttggaatcca cttagatgaa
     8881 caaatcccgg ttaatcaagt catcacccgg ctacaagtag aaggcttatt gacgttatca
     8941 gctggcgata atacgttacg tttgttaccc ccaattgtga tgcaaccggc cgatttatta
     9001 gctgggattg ccttgattgc aaaggtactg acaactttga caacggaggt cacgacgaat
     9061 gaataatcaa tttgaaggtc ggtcattctt gaaggaaatc gactatacac caacagaatt
     9121 agcttactta attgactttg ctagtcactt gaagaccctt aaagcacacc atatcccaca
     9181 cccttattta caaggaaaaa atattgcgtt gctgtttgaa aaaacgtcga cacggacacg
     9241 ttctgcgttt actgtggcgg ctaatgatct gggcgcgaat ccagagtttt tgggtaaaga
     9301 cgacatccaa ttcgggacga aggagtcagt tgttgatacg gcgaaagtac tggggtccat
     9361 gtatgatggc attgaatttc ggggctttaa acaaagcacg gtcgaagact tagcgaccta
     9421 tagtggtgtg ccagtctgga acgggttgac cgatgagtgg cacccgacgc agattttagc
     9481 ggatttttta acattaaagg aacattttgg tcatttaaaa gggttgacgt tagcgtacgt
     9541 tggtgatggt cgcaataata tggctaacag tttacttgtg gcgggagcga tgctcggcat
     9601 caatatcgct atcggggccc cggtagcgtt acagccagct ccagcagtgg tcgcgttggc
     9661 ccaacagtat gcgcaggcag ctgggagcac ggtcgtgatt acggctgatc cgcaacaagc
     9721 cgttactcag gctgatgcgc tttatacgga tgtctgggtc tcaatgggcg agcaggtcga
     9781 ctatggtgag cgaatcaagc aactattgcc gtttcaaatt aatactacgt tgctcgcagc
     9841 tactggcaag tcatcgacga ttgtcatgca ttgcttacca gcactacacg atctaaagac
     9901 gcagctagga gccaaattag gcgaacagta cggaatgacg gcgtttgaaa ttacggatga
     9961 tgtctttcag agtaagcagg cggtcgtctt tgaagaggcc ggcaaccgga tgccagcaat
    10021 taaggcggta atggcggcta cattaggtaa cttatttatt cctaccagca ttttcaatta
    10081 gcaagcttgc gggaactact agagcgtgct acgattatat tcgctttgtt tcaatcaata
    10141 cgtggttcgt aaccatccca cgttaaaaaa actaggagga attaagcgaa tgacacaatc
    10201 aaaaattcga ccatggatta tcaacgccct agtcgctgca atgtatgttg tattatgcct
    10261 aggccccgca gcttttagct tggcatcggg cgcaattcaa ttccgcgttt ctgaagggtt
    10321 gaaccactta gccgtgttca atcgcaagta tatttgggga attgttgctg gcgttatttt
    10381 gtttgacgct tttggtcccg gtgcgagtct cctcaacgtt ctctttggtg gcggccaatc
    10441 attattagcc ctgctggtat tgacgtggtt ggcccctaag ttgaagacgg tttggcaacg
    10501 gatgttactg aatatcgcgc tattcaccgt ttcgatgttt atgatcgcac tgatgattac
    10561 catgatgagt agtggtgtcg ctttttggcc gacatatcta acaactgctt tatcagagtt
    10621 gattatcatg agtatcactg cgccaattat gtacagcttg gatcgggtct tgcactttag
    10681 tgaccggatt cagtagggtg tgactgaatt taatattaag aacgaccggc gtagtatgtg
    10741 aagatggttt ttcgcatatt acgccggtct cttatttatt catttaatga aatgtggtta
    10801 atttgatttt tggttgatta ttttgaaatg gtcaacgcgt tttggattgt taactgctcg
    10861 gcagcaatga tcgtcctaat tcgaactgtt ttcagcagtc ttaagctggt cgcttttgaa
    10921 ctgaaaattc atctcataaa aaaagcccca aggtaacctc ggggcaactg tgattattca
    10981 gcttgtaaat cttccacttc gatgacttta aattgtttgc gattcaagtt gtcgataatt
    11041 cgtttgagct tttcactgtc gacattttct ggcaaggtaa agagcgtccg ataaccgagt
    11101 gtcccgtttt ggacgtctaa ggtcaggcag ccggcgattg aagtatattt cgtgataatc
    11161 ttagccatgg ctgcaagatc
//
