LOCUS       BGC0000900              9052 bp    DNA     linear   PAT 04-NOV-2005
DEFINITION  Ferrichrome biosynthetic gene cluster of koji mold.
ACCESSION   BGC0000900
VERSION     BGC0000900
KEYWORDS    .
SOURCE      Aspergillus oryzae
  ORGANISM  Aspergillus oryzae
            Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina;
            Eurotiomycetes; Eurotiomycetidae; Eurotiales; Aspergillaceae;
            Aspergillus; Aspergillus subgen. Circumdati.
COMMENT     OS   Aspergillus oryzae
            PN   JP 2003125779-A/10
            PD   07-MAY-2003
            PF   22-OCT-2001 JP 2001324181
            PI   HIROKI ISHIDA,YOJI HATA,AKITSUGU KAWATO,KOJI
            PI   SUGINAMI,YASUHISA
            PI   ABE,
            PI   MASAYUKI MACHIDA,MOTOAKI SANO
            PC   C12N15/09,C07K14/38,C12N1/15,C12N1/21,C12N9/10,C12N9/12// PC
            C12P21/02,
            PC   (C12N1/15,C12R1:66),(C12N1/21,C12R1:19),C12N15/00 CC
            Ferrichrome biosynthetic gene cluster of koji mold FH   Key
            Location/Qualifiers
            FT   source          1..9052
            FT                   /organism='Aspergillus oryzae'.
            ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 14:24:02
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 9052
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..9052
                     /db_xref="taxon:5062"
                     /mol_type="unassigned DNA"
                     /organism="Aspergillus oryzae"
     protocluster    1..9052
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[2044:7651]"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or
                     A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      2045..7651
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or
                     A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     cand_cluster    1..9052
                     /SMILES="NC([*])C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX))"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..9052
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000900"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..9052
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX))"
                     /subregion_numbers="1"
                     /tool="antismash"
     CDS             join(2045..7153,7283..7651)
                     /NRPS_PKS="Domain: AMP-binding (5-409). E-value: 1.6e-82.
                     Score: 269.2. Matches aSDomain:
                     nrpspksdomains_ctg1_orf000000_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (544-612). E-value: 2.5e-15. Score:
                     48.3. Matches aSDomain:
                     nrpspksdomains_ctg1_orf000000_PCP.1"
                     /NRPS_PKS="Domain: Condensation_DCL (650-921). E-value:
                     1.3e-48. Score: 157.5. Matches aSDomain:
                     nrpspksdomains_ctg1_orf000000_Condensation_DCL.1"
                     /NRPS_PKS="Domain: PCP (1346-1414). E-value: 1.1e-18.
                     Score: 59.1. Matches aSDomain:
                     nrpspksdomains_ctg1_orf000000_PCP.2"
                     /NRPS_PKS="Domain: Condensation_DCL (1482-1706). E-value:
                     1.5e-21. Score: 68.6. Matches aSDomain:
                     nrpspksdomains_ctg1_orf000000_Condensation_DCL.2"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1047:phosphopantetheine-binding domain-containing
                     protein (Score: 32.8; E-value: 1e-09)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg1_orf000000"
                     /sec_met_domain="AMP-binding (E-value: 1.4e-93, bitscore:
                     300.7, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 5.4e-38, bitscore:
                     117.8, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 2.3e-17, bitscore:
                     50.6, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=1
                     /translation="MHDVISEQSNRFPDKVAISSWDGELTYGQIEQYSTFVAGSLKKMG
                     VDVHDFIPVCFEKSRWTIVAVLAVMKAGGTLVMMDPTLPLARLQNMAKQVGAKAMVSSR
                     NQHELATTIMSDGKHIVVEADAFAHLSNSEPLPLLPAVPSSALMYIIFTSGSTGTPKGV
                     KISHETYTSSAFPRAKAVGYTEESRVLDFASYAFDVSIDSMLLTLANGGCLCIPSDEDR
                     LNDINEVIRRMKINYAGITPSVARILEPDVIASLSALGLGGEAAAATDVTRWGHDTRIV
                     IGYGPCECTIGCTVNSDTATGRDYISIGTGNGAAIWIADPNDHNVLMPVGAVGELLVEG
                     PIVGQGYLNDPDKTAAAFIHDPAWLVSGHNGYAGRSGRLYKTGDLGKYDPDGSGGIVFA
                     GRKDTQVKLRGQRVELGEIESQLNARLTAEITVIVEVIKPQSSGGQPTLVAFLASQSTK
                     KNGDTEIKSAELSDDLRKTLSKADAEIAKVLPRYMVPNAYISVNYIPVLISGKTDRKRL
                     REFGATVDLQQLDQGTNNGPARELTDLEQRLRHGWGQVLKLDPEGIRPNDNFFALGGDS
                     LAAMRLVSVCRAEGLDLSVTGTFGHPTLSAMASVVSIVDSKAKTETPPFSMISQAVESA
                     CLEASEACGSDPASVEDIYPCTPTQESLFTFSLKSVKPYIAQRVARIPSHISSDAWKKA
                     WGEVVAASPILRTRVAQLQERGLQQVVLKETISWRSSSDLVQYLETDRKERMSLGESLA
                     RYAIVSDPQTETRYMVWTIHHVVYDGWSEPVILKNVSDSLQGQSIETQAQIRDFVKWVR
                     DTDEVAMQEFWRRELKGAVGPQFPRLPARDYLPVPNAMVERQIPLETGSGWPFTLATLI
                     RGAWALVASQYTGSDDVVFGETLTGRDISLPGVESIVGPLIATVPIRVRIPRTSSVESY
                     LRDVQQSVVARTPYQHMGMQNIRKVSQDAQHACEAGTGLVIQPEPEYVGSELGFELGDV
                     VREALHFNPYPLMLGCGIRKGGFRICANFDSSLVEIPQMERILAQLEMACLQLTKGLSR
                     RVDEISCLPEAELDLIWQWNQIAPLSLDESSGRLRANTNIKQGSVYPPTVVSWVCDPRN
                     PSLLSPIGCVGRALARRALSFQEMPLKSPSWLVAGSSNFRGRTGRVQPTGDMVNLQEDR
                     SLVFVGRKENVVPVHGHAVDIADVESHFSKYLPPNVRAAAAVFQPSSDDTHSVTEQELA
                     VFIEQEPFEQDSVELLSAQYDITCEGSDTQNLSTSVRATISVSLLVALKRLSKFMQDSL
                     PSYMVPSAYVVLAKLPTDVGEVNHSLLKQLASNIPKHVLTQLREAFQQAWTKNLGQTNM
                     SLPESILRSAWAKILGISPEKIDVDDNFFRLGGDSVLAMKLVSSLRAQGHSLSVADIFQ
                     HMRLGDAAKVLKLGQVSQQKVQPYKAFSTLGHLDVEQFLSEIVRPKLADVTWSIQDVCP
                     VTDSQALDVRATIQGPRTSIQYTMLYLDKSIDREQLFRACNDLVKTHDILRTVFIEHES
                     TFYQVVLNELEVPVAMHQADKDLGPYIKDLCTSHTESFHLGSSFFKLLHVEGNDGQECL
                     ILGLSHAQYDGMSLPRLLQDLETLYTGGKIVDFEPFSAYIARISDEGVQTKAINYWRNL
                     LNGSSLSVLEGTSVQPTDKAIFHTKPVDVSQPLDLDEVTTANLLTAAWALVLACRLQKP
                     DVTFGGRQSAESAAHDFLGFNKISKKCTQWSSEATCFDSLVHHQDWDDFDTMPFAGGTC
                     KVDILNPDGDAPYPLKVVSFVRGGQLNVGVAGSERDAAFVDAALDELAATVKELATRPS
                     EHVLIDGHMF"
     aSDomain        2060..3271
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg1_orf000000_AMP-binding.1"
                     /evalue="1.60E-82"
                     /label="ctg1_orf000000_AMP-binding.1"
                     /locus_tag="ctg1_orf000000"
                     /protein_end="409"
                     /protein_start="5"
                     /score="269.2"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="SEQSNRFPDKVAISSWDGELTYGQIEQYSTFVAGSLKKMGVDVHD
                     FIPVCFEKSRWTIVAVLAVMKAGGTLVMMDPTLPLARLQNMAKQVGAKAMVSSRNQHEL
                     ATTIMSDGKHIVVEADAFAHLSNSEPLPLLPAVPSSALMYIIFTSGSTGTPKGVKISHE
                     TYTSSAFPRAKAVGYTEESRVLDFASYAFDVSIDSMLLTLANGGCLCIPSDEDRLNDIN
                     EVIRRMKINYAGITPSVARILEPDVIASLSALGLGGEAAAATDVTRWGHDTRIVIGYGP
                     CECTIGCTVNSDTATGRDYISIGTGNGAAIWIADPNDHNVLMPVGAVGELLVEGPIVGQ
                     GYLNDPDKTAAAFIHDPAWLVSGHNGYAGRSGRLYKTGDLGKYDPDGSGGIVFAGRKDT
                     QVKLR"
     aSModule        2060..3880
                     /complete
                     /domains="nrpspksdomains_ctg1_orf000000_AMP-binding.1"
                     /domains="nrpspksdomains_ctg1_orf000000_PCP.1"
                     /locus_tags="ctg1_orf000000"
                     /monomer_pairings="X -> X"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       2486..2545
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg1_orf000000_0001"
                     /evalue="5.10E-09"
                     /label="NRPS-A_a3"
                     /locus_tag="ctg1_orf000000"
                     /protein_end="167"
                     /protein_start="147"
                     /score="28.2"
                     /tool="antismash"
                     /translation="MYIIFTSGSTGTPKGVKISH"
     CDS_motif       3029..3118
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg1_orf000000_0002"
                     /evalue="2.50E-15"
                     /label="NRPS-A_a6"
                     /locus_tag="ctg1_orf000000"
                     /protein_end="358"
                     /protein_start="328"
                     /score="48.5"
                     /tool="antismash"
                     /translation="PVGAVGELLVEGPIVGQGYLNDPDKTAAAF"
     CDS_motif       3236..3301
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg1_orf000000_0003"
                     /evalue="9.50E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="ctg1_orf000000"
                     /protein_end="419"
                     /protein_start="397"
                     /score="36.8"
                     /tool="antismash"
                     /translation="FAGRKDTQVKLRGQRVELGEIE"
     aSDomain        3677..3880
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg1_orf000000_PCP.1"
                     /evalue="2.50E-15"
                     /label="ctg1_orf000000_PCP.1"
                     /locus_tag="ctg1_orf000000"
                     /protein_end="612"
                     /protein_start="544"
                     /score="48.3"
                     /tool="antismash"
                     /translation="EQRLRHGWGQVLKLDPEGIRPNDNFFALGGDSLAAMRLVSVCRAE
                     GLDLSVTGTFGHPTLSAMASVVS"
     CDS_motif       3746..3775
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg1_orf000000_0004"
                     /evalue="4.30E-01"
                     /label="PCP_mE"
                     /locus_tag="ctg1_orf000000"
                     /protein_end="577"
                     /protein_start="567"
                     /score="4.3"
                     /tool="antismash"
                     /translation="NFFALGGDSL"
     aSDomain        3995..4807
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg1_orf000000_Condensation_DCL.
                     1"
                     /domain_subtypes="Condensation_DCL"
                     /evalue="1.30E-48"
                     /label="ctg1_orf000000_Condensation_DCL.1"
                     /locus_tag="ctg1_orf000000"
                     /protein_end="921"
                     /protein_start="650"
                     /score="157.5"
                     /tool="antismash"
                     /translation="EDIYPCTPTQESLFTFSLKSVKPYIAQRVARIPSHISSDAWKKAW
                     GEVVAASPILRTRVAQLQERGLQQVVLKETISWRSSSDLVQYLETDRKERMSLGESLAR
                     YAIVSDPQTETRYMVWTIHHVVYDGWSEPVILKNVSDSLQGQSIETQAQIRDFVKWVRD
                     TDEVAMQEFWRRELKGAVGPQFPRLPARDYLPVPNAMVERQIPLETGSGWPFTLATLIR
                     GAWALVASQYTGSDDVVFGETLTGRDISLPGVESIVGPLIATVPIRVRI"
     aSModule        3995..6286
                     /domains="nrpspksdomains_ctg1_orf000000_Condensation_DCL.1"
                     /domains="nrpspksdomains_ctg1_orf000000_PCP.2"
                     /incomplete
                     /locus_tags="ctg1_orf000000"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       4064..4168
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg1_orf000000_0005"
                     /evalue="3.00E-05"
                     /label="C2_DCL_024-062"
                     /locus_tag="ctg1_orf000000"
                     /protein_end="708"
                     /protein_start="673"
                     /score="16.3"
                     /tool="antismash"
                     /translation="YIAQRVARIPSHISSDAWKKAWGEVVAASPILRTR"
     CDS_motif       4358..4411
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg1_orf000000_0006"
                     /evalue="8.40E-06"
                     /label="C3_DCL_135-156"
                     /locus_tag="ctg1_orf000000"
                     /protein_end="789"
                     /protein_start="771"
                     /score="18.4"
                     /tool="antismash"
                     /translation="IHHVVYDGWSEPVILKNV"
     CDS_motif       4703..4798
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg1_orf000000_0007"
                     /evalue="3.20E-13"
                     /label="C5_DCL_263-294"
                     /locus_tag="ctg1_orf000000"
                     /protein_end="918"
                     /protein_start="886"
                     /score="42.1"
                     /tool="antismash"
                     /translation="DVVFGETLTGRDISLPGVESIVGPLIATVPIR"
     CDS_motif       5552..5617
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg1_orf000000_0008"
                     /evalue="1.90E-02"
                     /label="NRPS-A_a8"
                     /locus_tag="ctg1_orf000000"
                     /protein_end="1191"
                     /protein_start="1169"
                     /score="8.0"
                     /tool="antismash"
                     /translation="FVGRKENVVPVHGHAVDIADVE"
     aSDomain        6083..6286
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg1_orf000000_PCP.2"
                     /evalue="1.10E-18"
                     /label="ctg1_orf000000_PCP.2"
                     /locus_tag="ctg1_orf000000"
                     /protein_end="1414"
                     /protein_start="1346"
                     /score="59.1"
                     /tool="antismash"
                     /translation="ESILRSAWAKILGISPEKIDVDDNFFRLGGDSVLAMKLVSSLRAQ
                     GHSLSVADIFQHMRLGDAAKVLK"
     CDS_motif       6152..6181
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg1_orf000000_0009"
                     /evalue="3.40E-01"
                     /label="PCP_mE"
                     /locus_tag="ctg1_orf000000"
                     /protein_end="1379"
                     /protein_start="1369"
                     /score="4.6"
                     /tool="antismash"
                     /translation="NFFRLGGDSV"
     aSDomain        join(6491..7153,7283..7291)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg1_orf000000_Condensation_DCL.
                     2"
                     /domain_subtypes="Condensation_DCL"
                     /evalue="1.50E-21"
                     /label="ctg1_orf000000_Condensation_DCL.2"
                     /locus_tag="ctg1_orf000000"
                     /protein_end="1706"
                     /protein_start="1482"
                     /score="68.6"
                     /tool="antismash"
                     /translation="YTMLYLDKSIDREQLFRACNDLVKTHDILRTVFIEHESTFYQVVL
                     NELEVPVAMHQADKDLGPYIKDLCTSHTESFHLGSSFFKLLHVEGNDGQECLILGLSHA
                     QYDGMSLPRLLQDLETLYTGGKIVDFEPFSAYIARISDEGVQTKAINYWRNLLNGSSLS
                     VLEGTSVQPTDKAIFHTKPVDVSQPLDLDEVTTANLLTAAWALVLACRLQKPDVTFGGR
                     QS"
     CDS_motif       6518..6589
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg1_orf000000_0010"
                     /evalue="8.10E-06"
                     /label="C2_LCL_024-062"
                     /locus_tag="ctg1_orf000000"
                     /protein_end="1515"
                     /protein_start="1491"
                     /score="18.3"
                     /tool="antismash"
                     /translation="IDREQLFRACNDLVKTHDILRTVF"
     CDS_motif       6806..6856
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg1_orf000000_0011"
                     /evalue="1.20E-02"
                     /label="C3_LCL_132-143"
                     /locus_tag="ctg1_orf000000"
                     /protein_end="1604"
                     /protein_start="1587"
                     /score="8.4"
                     /tool="antismash"
                     /translation="YDGMSLPRLLQDLETLY"
     CDS             complement(8479..8911)
                     /locus_tag="ctg1_orforf00001"
                     /transl_table=1
                     /translation="MRT"
ORIGIN
        1 ccgccgccaa gaccacgata aattgattaa tagtactaaa aatccactaa gtcaggcggt
       61 catttcttgt atcaataaat tagtatgtta ggaggggtga ctactctgta gaggacttgg
      121 cggttctcta aaacgtcgtt gttactattt ttctctcgac attcctctcc atgagctttc
      181 attctaccaa atgggtccag ggtcctgcag agatcatctc tatatttaac atgacatggc
      241 tgtcaccgtt gatcctgctc cctacagagt actgatcagc tatacaccct catcttggcg
      301 tctttgtgtt taaccgtact ggagaataca tgttacagta tttgtaccct agaaagattt
      361 atcagatact agggtatttc cgataggtcg gcccacggtt tttttggtaa taaatcccgt
      421 acggattgta tcttggcgtc ttggcccgca taaaatccga gttgtcaaaa ataaaaagag
      481 aaaaggaaaa agggaagaag agcagaagac gaagattccg ttcagagtcc tctgctcttg
      541 acttttctct gcgagatctt tttactcttg gctatgaata tatgccattt caacatgacg
      601 atatgcaaat attacacaac cataatgtgt cttatgccag tgtataaatg atgcagattt
      661 tcagcggcta agacggatat cattttccgc ctggtattat gatcaggtga tgcgggcacc
      721 cctaattcgc acgccagtat cagaacttta gctccctcac gagtaaaaag aaaaaagaaa
      781 ctgaaaatac cgttttgtaa cttgtttact gtttccagtt ctcaagcgag acataccgta
      841 catacatacc atattttcgt actgtacagg ctgtattgta cctgaacagc attggggccc
      901 gacactcttc cactgcgcaa ttatgggtcc catccgttca tcggacacgc ggatacggag
      961 tacatacaca tactctgctc tacttgcgtc caagagacat gtgggcatct gactagccca
     1021 cagcctcctg ccatggcgat ctcggtcaga ctctacagag tacctgcagg tacggagtta
     1081 ttcaggttac tttccggctc gtccgtcggt ggaggacaat gctagcacaa ccttggttgc
     1141 ttagttatat aacttgcgcc ttaccacttc agtgcggtcc ggctagtcca aagctccagc
     1201 aacttgaacg gccggccgag gtctaacccc tcttcgtacc cttatacccc gccaagattt
     1261 cgagtccagg taggctgggc tgccggaaga atggctatac aggttgatgg tacacataat
     1321 caaagccaac acagaaatgg gtctgttggc gaataatctg ccggtcaggc tgatggtgac
     1381 agtttcacat catttgaggt cgtcggcctt gcagccgacg agcccaccac tcccactagc
     1441 atgaaggagg aagttttgct tatctcatgg cttattgtgc tcttgcgaac ccgcgatagt
     1501 ggtcaggtcc aatatgaagt gggcgtacaa ggggcgagat aatggggctt gaacaggagc
     1561 cagtaaaagc atgtttgtcc gatgggggaa ttattgacag gattgcaaga tagtgtcgga
     1621 caagccacgg cagcaatttc tcaacatgtt aagaccgtca cgtcaagtga gtgtacgact
     1681 gtgtctaacc ctgcctcact cctgctgagt acgggttccc tgtcgcaaac atcagaagat
     1741 gtaaagatga agtgagtgat gaagttttcg ttgtatttca agtacctaac cggtgcggaa
     1801 aaagggtgca attcacctcg agacacgctt tgagaatggc cgactcgcga ttcgcccagc
     1861 ttggcgctcc gaaacgatgt tagaattcac agtaacacaa cacatcaagt cgctggtcga
     1921 cactgtcaag atgtgtctgt caaatcccga tgcgtccatt gaagcctgcc ttcggccaac
     1981 cgcgagtgat ttggatgcga tatggaaatg gaatcacgaa cttcctccct catacaactt
     2041 ctgcatgcac gatgtgattt ccgaacaatc gaacagattc ccggacaagg tggcgatctc
     2101 atcatgggat ggcgagttga cgtatggcca aatcgaacag tattccacat ttgtggcagg
     2161 ctcgttgaaa aaaatgggtg tggatgtaca tgatttcata ccggtttgct ttgagaaatc
     2221 aaggtggaca atcgtcgctg tacttgcggt gatgaaagca ggaggaactt tggtgatgat
     2281 ggacccaaca cttccgctcg ctcgtctgca gaacatggct aaacaagtcg gtgcgaaggc
     2341 aatggtatcg tcccgcaacc agcatgagct ggcaacaact atcatgtcag acggaaagca
     2401 catcgttgtg gaagctgacg catttgcaca cctgtcaaat tcggaacccc ttccactgtt
     2461 gccggcagtc ccctcatctg ccctaatgta catcatcttc acttctggca gcacgggtac
     2521 gcccaaagga gtcaagatct ctcacgaaac atatacaagc agcgctttcc cacgagccaa
     2581 ggctgtgggt tatacggagg agtccagggt cctcgatttt gcatcctacg ctttcgatgt
     2641 cagtatcgac agcatgcttt tgacgttggc aaacggcggt tgcctttgta ttccctccga
     2701 tgaggatcga ctgaacgata ttaatgaagt gatacgaaga atgaaaatca actacgcagg
     2761 tataactcct tctgtggcca gaattctgga gcctgatgta atagcatcgc tgagtgctct
     2821 tggtctggga ggagaggcag cagcagcaac ggatgtgacg cgctggggcc atgacactag
     2881 gattgtcata ggttacggcc cctgtgagtg cacgatcgga tgtacggtga acagcgatac
     2941 tgccactggt agagactata tctctattgg gacaggaaac ggcgcagcca tctggattgc
     3001 cgaccctaac gaccacaatg tcttaatgcc agtcggcgct gtgggcgagc tactcgttga
     3061 aggtccgatt gtaggacagg gttacttgaa tgatccagac aaaaccgcag cggctttcat
     3121 ccacgaccca gcttggcttg tctctggcca caatggctac gctggtcgtt cggggcgtct
     3181 gtacaagact ggagaccttg gaaagtatga tccggacgga tctgggggca tcgtctttgc
     3241 tgggagaaaa gacacacaag tcaaactgcg tggacaacgt gtcgagttgg gcgaaatcga
     3301 aagccagctt aacgccagac taactgcaga aatcaccgtg attgtggagg tgattaagcc
     3361 tcagtcttct gggggtcagc ccacgttggt ggcattcctt gcgtcccagt ctacgaagaa
     3421 gaatggtgat accgaaataa agtcagcaga gctatccgac gacctgcgca aaacgctgtc
     3481 caaagccgat gcagagatag caaaggtttt gccgcgttat atggtgccaa acgcatatat
     3541 ttcggtcaat tacattcctg ttttaatttc gggaaagacg gaccgcaaac ggctccgtga
     3601 attcggcgcc acagtagact tacaacagtt ggatcaaggc acaaacaacg ggccagcacg
     3661 ggagttaacg gaccttgagc aacggttgcg tcacggttgg ggccaagtat tgaaactaga
     3721 tcccgaaggc attcggccta acgacaattt cttcgcactc ggtggtgact ccctggcggc
     3781 catgagactt gtgtctgtct gcagagcgga gggtctcgat ctcagtgtca ctggcacctt
     3841 tggccaccct actctctcag cgatggccag tgtcgtcagc attgtcgact caaaggcaaa
     3901 gacagaaaca ccgccatttt caatgatctc tcaagctgtc gagagcgcct gtctggaagc
     3961 atcagaagcc tgcggatcag atcctgcatc cgtcgaagac atctaccctt gtacacccac
     4021 gcaagagtcg cttttcacct tctcgctcaa gtcagtcaag ccatatattg cccagagagt
     4081 cgcacgcatt ccctcacaca tcagttctga tgcttggaaa aaggcatggg gtgaggttgt
     4141 cgcggcaagc cccatcctgc gcactcgagt ngcccagctg caggaacgtg gtcttcagca
     4201 agtcgtgcta aaggaaacta tctcctggag gtcttcatcc gatcttgtac agtacctaga
     4261 gactgatcgg aaggagagaa tgagtcttgg agaaagtcta gctcgatacg ccatagttag
     4321 cgatccacag actgagacga ggtatatggt ctggacgatt catcacgtgg tctacgacgg
     4381 gtggtcagag ccagtaatac ttaagaatgt cagtgattcc ttgcaaggcc agtcgattga
     4441 aacccaggct caaatcagag acttcgtcaa atgggtacgc gatacagatg aagttgccat
     4501 gcaggaattc tggagacggg aattaaaagg cgcggtcgga cctcaattcc ctcgattgcc
     4561 cgcgagagac tacttgcccg ttccgaatgc catggtcgaa cgtcagatac cgctcgagac
     4621 aggttccggt tggcctttca cactggcaac acttattcgc ggtgcctggg cccttgtagc
     4681 gtcgcagtat acagggagcg acgatgttgt gttcggcgaa acacttaccg ggcgagacat
     4741 ttcgttgcca ggagtggaaa gcattgtggg cccattgatt gcaacagtac ctatccgcgt
     4801 acgtatacct cgaaccagct cagtcgaatc ttatttgcgg gatgtgcagc aaagcgttgt
     4861 ggcccgtacc ccgtatcagc atatggggat gcaaaacatt cggaaggtca gccaagacgc
     4921 gcaacatgca tgtgaagctg gtacaggctt ggtcatccag ccagagccgg agtatgtggg
     4981 cagcgaactt ggctttgagc tcggagacgt cgtacgtgaa gcgctgcatt tcaacccgta
     5041 tccgcttatg ttaggctgtg gtatccgaaa gggtggtttc agaatatgcg caaatttcga
     5101 tagctccttg gtggagatcc cacaaatgga acgaatcctc gcgcagctcg aaatggcttg
     5161 cttgcaattg acaaaaggcc tctctagaag ggtagacgag atatcctgct tgcccgaggc
     5221 ggaactggat ctgatttggc aatggaatca aattgctccc ttgtccttgg atgaatcgtc
     5281 cggaaggctt cgagcaaaca caaacatcaa gcaaggatcg gtttacccac ctacggtggt
     5341 ttcctgggtg tgtgacccga ggaacccgtc gttgctatcg ccaatcggct gtgttgggcg
     5401 agctctggct cgaagggcgc tttcctttca ggagatgcca ttgaaatccc cctcctggct
     5461 cgtggctggc agctccaatt ttagaggccg gacgggaaga gtgcagccga ctggagacat
     5521 ggttaattta caagaggata gaagcttagt atttgttggc cgaaaggaaa atgttgtgcc
     5581 agttcacggg catgcggtgg atatcgccga tgtcgaatct cacttttcaa agtacctccc
     5641 accaaatgtt cgtgctgcgg ctgccgtctt ccaaccatct tcagatgaca cccactctgt
     5701 aacagaacaa gagctggccg tattcataga gcaagagcct tttgagcaag acagtgttga
     5761 gctcctatcg gcgcaatatg acatcacttg tgagggttca gacacgcaaa acctctcgac
     5821 gtctgttcgt gccacaattt ccgttagtct tctcgttgca ctgaagcgac tcagcaagtt
     5881 tatgcaagac tcccttccat catacatggt gccctcagcc tacgttgttc ttgccaaatt
     5941 gcccactgat gtgggtgaag tcaaccacag tttgctcaaa cagctagcct cgaatattcc
     6001 taaacacgta ctcacccaac ttcgtgaagc cttccagcag gcctggacga agaacttagg
     6061 gcaaactaat atgtcccttc cagagagtat cctgcggtcc gcctgggcaa agatactagg
     6121 aatcagtccc gagaagattg atgtcgacga taacttcttc cgcctgggtg gtgattccgt
     6181 tttagcgatg aaactggtct caagccttcg tgcacagggg cacagtttgt cagtggctga
     6241 catcttccaa cacatgcgtc ttggtgatgc agctaaagta ttgaaactgg gccaggtatc
     6301 gcaacagaaa gtccagcctt acaaggcttt ctcgactctg ggccatttgg atgtagagca
     6361 gttcttgtcc gaaattgtcc ggccgaagct tgctgatgtt acgtggtcca tccaggacgt
     6421 atgtccggtg acagactccc aagcactcga cgtcagagct accatccagg gaccgcgcac
     6481 ctcgatacaa tatactatgt tgtacctgga caaaagcatc gatcgagagc aattgttccg
     6541 cgcctgcaac gacctggtca agactcacga cattctgcga actgtcttca tcgaacacga
     6601 atctactttc taccaggttg tgttgaatga gttggaagtt cctgtagcca tgcaccaagc
     6661 tgataaggat cttggtccat acatcaagga tctttgtaca tcacacacag aatccttcca
     6721 cctaggatcg tccttcttca aattgctcca tgtcgagggc aacgatggcc aagaatgcct
     6781 catcttaggt ctttcacacg ctcagtacga cggaatgtct ctgcctaggc tgctacaaga
     6841 tctggaaact ttgtacactg gcggaaagat tgtcgacttc gagccgttct cagcatacat
     6901 tgcacggatt tctgatgagg gtgtccaaac taaagcaatc aactactggc gcaacctttt
     6961 gaacgggtca tccctatctg ttctggaggg aacgtcagta cagcctacag acaaagccat
     7021 attccatact aagcccgtcg atgtctccca gcccctcgac ctcgacgagg tcacgactgc
     7081 gaacttgctc accgcagctt gggcactggt actggcatgc cgtctccaaa aacccgacgt
     7141 gacattcggt ggcgtgactt caggccgaaa tatcgacctc gcaaatgtgg aaaatgtaat
     7201 gggtcactgc taccaattaa ccccgattag agtcgcgttc cagtcacagt ggaccgcgat
     7261 ggatctactg cgctttgttc agaggcaaag cgccgaatct gcagctcacg acttcctcgg
     7321 attcaataag atctccaaga aatgcacgca atggtcatcg gaagcaacat gtttcgactc
     7381 cctcgtccat catcaggact gggatgattt tgacaccatg ccttttgccg ggggaacttg
     7441 caaggttgat attctgaacc cagatgggga tgccccttat cctttgaagg ttgtttcatt
     7501 tgtccgtggt gggcaattga atgtcggagt cgctggaagt gaaagagatg cggcgttcgt
     7561 ggacgcagcc cttgatgagt tggctgccac ggtcaaggag ctggcgacgc gtccgtcgga
     7621 gcacgtgttg attgacggtc acatgttcta gacccaagac tggtcaaagt cgccgatacc
     7681 tccctcgaca ttggttcatg gataacgatt tcatttggtt ctttttgcat gtatttctct
     7741 taggagtcga ttttcttgtt atatcctagt agtatatcat tagaaatgtc aaatccagct
     7801 ctaaaacaaa tgttcttggc acctcccgct gtttttcccc aaaagtcacg ggacagtgta
     7861 taatgaggtc atattggaca aacctgtata ttaagagtct gttggaggaa gtcattgtgc
     7921 catagctaaa gatttatgac tcgaacttaa gtcaccatta ttatcgttgt attttaccta
     7981 ccgtagaagt gtgagaatag ggctagttgt taagatatat tttctgtccg tgcaacccta
     8041 aaatacatac ggccatatag ctgtaccttt agttttggag attttggaca attcagctga
     8101 gatattcggg acttgtacaa cgtattcaat aattacaata agaattgtgt aaataaattc
     8161 ttactgttct ttcttttgta cctcggtgat tgagctgtca agccaagcgg cttgaatctt
     8221 caaccacgca aggtacatag attttaccac cgccgagcta tggtagagat gaaggctaga
     8281 ctgtactttt ctctcattgt cttgcaaaac ataaaaacca caaattgaaa tattataata
     8341 tatagattta aaaaaaggtg ccgctattac aaatatatag aaagtctatc cggggaataa
     8401 tttaaaaaca gtctctgcag atatgcaatc aaaatggtcc aaaaatcgca acaatggttt
     8461 atccggcaca atccacgatc acgtggccac tgtttgatgc atgacgtttg ctctgtagct
     8521 gtccgatctt tcgagcaact cctcgtgcga tccgcgatca atgcatttac ccccttcaat
     8581 caagaagatg acatctgctg ctctaattgt gtgaagacga tgggcgatag caatgacagt
     8641 aatgcccttt gcggcctttt cgagtccgtc ttgcagtagc ttctccgatt cagcgtccaa
     8701 agcgctggtc gattcatcga gaatcagaag cttaggcttc cgaacaaggg cccgagcaat
     8761 agacaacctc tgcttctgac ctcccgagaa ctggctacca ttaggtccgc aaagagtctt
     8821 gtagccctct ggcagcgcct ctataacgtc gtggatgttg gcaagcttgc atgcctcttt
     8881 gatttctcga tagtcgcttt catgtcctgg tctagcacca aggccatgtt gaactcaata
     8941 ctgccctcaa aaagcacgct ttcttgtggc acaagagcga tagagtcgcg gaagtcaacg
     9001 cctcggtgcc tggtaacgtc gacgccatcg atcacgatgg atccggtttc gg
//
