LOCUS       BGC0000913             11029 bp    DNA     linear   BCT 26-JUL-2016
DEFINITION  Enterobacter cloacae NADH dehydrogenase subunit N gene, partial cds;
            putative chemotaxis signal trancription protein, ribonuclease Z,
            GCN-related N-acetyltransferase, and ElaB (elaB) genes, complete
            cds; menaquinone biosynthesis gene cluster, complete sequence; and
            TctA (tctA) gene, partial cds.
ACCESSION   BGC0000913
VERSION     BGC0000913
KEYWORDS    .
SOURCE      Enterobacter cloacae
  ORGANISM  Enterobacter cloacae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 14:25:22
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 11029
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..11029
                     /db_xref="taxon:550"
                     /mol_type="genomic DNA"
                     /organism="Enterobacter cloacae"
                     /strain="SLD1a-1"
     subregion       1..11029
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000913"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..11029
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     CDS             <1..349
                     /codon_start=2
                     /product="NADH dehydrogenase subunit N"
                     /protein_id="ACD75086.1"
                     /transl_table=11
                     /translation="MLSAVMTVMMLSLAGIPMTLGFIGKFYVLAVGVQAHLWWLTAGVV
                     IGSAIGLYYYLRVAVSLYLSAPQQLNRDAPGNWQYSAGGIVVLISALLVLIFGIYPQPL
                     IDIVQRAMPLM"
     CDS             complement(442..1446)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1201:response
                     regulator (Score: 118.2; E-value: 6.2e-36)"
                     /gene_kind="regulatory"
                     /note="CheW"
                     /product="putative chemotaxis signal trancription protein"
                     /protein_id="ACD75087.1"
                     /transl_table=11
                     /translation="MDNFQKDIDDRANLTLSNRFELLLFRLGTSLNENKSELFGINVFK
                     LREIVPMPEFTKPAGMKSPLMGMVNIRDQVIPVIDLAAVAGCKPTTGLNILLITEYARS
                     VQAFAVESVENIMRLDWKQVHAAETAVSGRYITSIACLDEKTDTNELAMVLDVEQILYD
                     ITPANHDLHATNLQTTKFHIKPGAVAIVAEDSKVARSMLEKGLQAMEIPAQLHITGKDA
                     WEKIGVLAAQAQAEGVPITDKIALVLTDLEMPEMDGFTLTRKIKTDPLLKDIPVVIHSS
                     LSGNANEDHIRKVKADGYVAKFELNELSSVIEEVLDRSMKKIDGPLISRKQLA"
     CDS             complement(1511..2428)
                     /codon_start=1
                     /product="ribonuclease Z"
                     /protein_id="ACD75088.1"
                     /transl_table=11
                     /translation="MELIFLGTSAGVPTRTRNVTAILLDLQHPTRGGLWLFDCGEGTQH
                     QLLHTAYHPGKLDKIFITHLHGDHLFGLPGLLCSRSMAGNANPLTIYGPAGTAEFVETT
                     LRLSGSWTDYPLEVIEITEGLVLDDGDYTVTARPLNHPVECYGYRIEAHDKPGALDAAA
                     LIADGVKPGPLFQRLKQGETVTLEDGRTINGRDYLAPPQPGKKLAIFGDTAPCPAALQL
                     AREVDVMVHEATLETAMEEKANSRGHSSTRQAAALAREANVGRLIVTHVSSRYDARGCE
                     KLLTECRDVFPACELANDFTKVSV"
     CDS             2499..2960
                     /codon_start=1
                     /product="GCN-related N-acetyltransferase"
                     /protein_id="ACD75089.1"
                     /transl_table=11
                     /translation="MIQWQDLHHSELTVQTLYALLKLRCEVFVVEQTCPYQDIDGDDLV
                     GENRHILGWRDKELVAYARILKSEDDFEPVVIGRVIISGKARGEKLGYQLMEKTLEACQ
                     KQWPDKALYLGAQAHLQSFYGHFGFMPVTDVYDEDGIPHIGMAREVKQA"
     gene            3014..3319
                     /gene="elaB"
     CDS             3014..3319
                     /codon_start=1
                     /gene="elaB"
                     /note="putative inner membrane protein"
                     /product="ElaB"
                     /protein_id="ACD75090.1"
                     /transl_table=11
                     /translation="MSFQSWDTRVDDDLTLLSETLEEVLRSSGDPADQKYIELKARAEQ
                     ALHDVKTRVSQASDNYYYRAKKAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            3415..4710
                     /gene="menF"
     CDS             3415..4710
                     /codon_start=1
                     /gene="menF"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1018:isochorismate synthase (Score: 241.2; E-value:
                     4.6e-73)"
                     /gene_kind="biosynthetic-additional"
                     /note="isochorismate synthase"
                     /product="MenF"
                     /protein_id="ACD75091.1"
                     /transl_table=11
                     /translation="MHSISNALERLRTQLAQALPATPGLRHVDVSFPLNDAFDPLAWLG
                     AQCLFPQFYWQQRNGDEELAALGAVIHFSSLAQASQFLQQQPVANDTRICGLNAFTPEQ
                     GSLFLPRLLWRRSAGTATLRLQLWSDRSLQEDADAALAFLQQLRDARPIRPLSAQVVQE
                     THQPQKPEWLRLIRQATETIARGEVEKVVLARATDLQFSQPVNAVALMAASRALNLNCY
                     HFCMVFDASNAFLGSTPERLWRRRGNLLRTEALAGTVASHPDDKQARLLGDWLMNDDKN
                     QRENMLVVEDICQRLQHHTRTLEVLPAQVMRLRKVQHLRRCIWTELRHADDEQCLHVLQ
                     PTAAVAGLPRQPAREFIHRVEPFDREWYAGSAGYLSPAQSEFCVALRSARVHDATVRLY
                     AGAGIVSGSDPEQEWQEIENKAAGLRSLLLKD"
     misc_feature    3415..10419
                     /note="menaquinone biosynthesis gene cluster"
     gene            4799..6469
                     /gene="menD"
     CDS             4799..6469
                     /codon_start=1
                     /gene="menD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TPP_enzyme_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TPP_enzyme_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1055:pyruvate oxidase/decarboxylase (Score: 131.8;
                     E-value: 5.3e-40)"
                     /gene_kind="biosynthetic-additional"
                     /note="2-succinyl-6-hydroxyl-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /product="MenD"
                     /protein_id="ACD75092.1"
                     /sec_met_domain="TPP_enzyme_N (E-value: 8.4e-24, bitscore:
                     73.8, seeds: 75, tool: rule-based-clusters)"
                     /sec_met_domain="TPP_enzyme_C (E-value: 2.1e-12, bitscore:
                     36.8, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSVSSFNRRWATVILEALTRHGVRHVCIAPGSRSTPLTLAAAENR
                     ALIHHTHFDERGLGHLALGLAKVSKEPVAVIVTSGTAVANLYPALIEAGLTGEKLVLLT
                     ADRPPELIDCGANQAIRQPGLFASHPSQTVSLPRPTQDIPASWLVSTVDHAMETLRSGA
                     LHINCPFAEPLYGEMDDTGLAWQQQLGDWWESEKPWLREQTHLESAKQRDWFFWRQKRG
                     VVIAGRMSAAEGRQVAEWAHTLGWPLIGDVLSQTGQPLPCADLWLGNAKAVTELAQAQI
                     VVQLGASLTGKRLLQWQATCSPEEYWLVDPLEGRLDPAHHRGRRLVSDIDTWLALHPAE
                     KRAPWAVEIPALSRQAWELTRAQCEEFGEAELAHRIHRYLPEQGQLFVGNSLVVRLIDA
                     FSKLPAGYPVYSNRGASGIDGLISTAAGVQRASAKSTLAIVGDLSALYDLNALALLRQV
                     SAPFVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVQFEHAAAMFNLKYHRPENWEAL
                     DAALSSAWRQPGATLIELVVNEADGAQKLQSLLAQVSHL"
     gene            6466..7242
                     /gene="yfbB"
     CDS             6466..7242
                     /codon_start=1
                     /gene="yfbB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1036:alpha/beta hydrolase fold protein (Score: 56.9;
                     E-value: 4.3e-17)"
                     /gene_kind="biosynthetic-additional"
                     /note="acyl-CoA thioester hydrolase"
                     /product="YfbB"
                     /protein_id="ACD75093.1"
                     /sec_met_domain="PF00561 (E-value: 1.1e-15, bitscore: 47.6,
                     seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="Abhydrolase_6 (E-value: 7e-19, bitscore:
                     58.9, seeds: 455, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MILAGAQQAGKPGYPWLVFLHGFSGDCREWQTVGEALSDYPRLYL
                     DLPGHGRSASMAVTGFDEMSDVLNRTLVSYNILNYWLVGYSLGGRIAMFHACQDPAGLC
                     GIMVEGGHPGLKGVAAREARAASDHLWAQRFRTQPLSNVFADWYQQPVFASLTDSQRRA
                     LIALRCRNAGTNLAMMLEATSLAVQPDLRAALSARHFPFDYLYGERDEKFAALAAELNA
                     VRHVIPNAGHNAHRENPGAVSASLAQILRRRIKDTL"
     gene            7239..8096
                     /gene="menB"
     CDS             7239..8096
                     /NRPS_PKS="Domain: ECH (30-273). E-value: 1.2e-69. Score:
                     226.2. Matches aSDomain: nrpspksdomains_menB_ECH.1"
                     /codon_start=1
                     /gene="menB"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1023:enoyl-CoA hydratase (Score: 215.8; E-value:
                     8.6e-66)"
                     /gene_kind="biosynthetic-additional"
                     /note="nathphoate synthase"
                     /product="MenB"
                     /protein_id="ACD75094.1"
                     /transl_table=11
                     /translation="MIYPDENMLYAPVEWQDCSEGYTDIRYHKSADGIAKITINRPQVR
                     NAFRPLTVKEMIQALADARYDDSIGVIVLTGEGEKAFCSGGDQKVRGDYGGYQDDAGTH
                     HLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAAENAIFGQTGPKVGSFD
                     GGWGASYMARIVGQKKAREIWFLCRQYNAQEALDMGLVNTVVPIADLEKETVRWCREML
                     QNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNEKRQPDFSKY
                     KRNP"
     aSDomain        7329..8057
                     /aSDomain="ECH"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_menB_ECH.1"
                     /evalue="1.20E-69"
                     /label="menB_ECH.1"
                     /locus_tag="menB"
                     /protein_end="273"
                     /protein_start="30"
                     /score="226.2"
                     /tool="antismash"
                     /translation="ADGIAKITINRPQVRNAFRPLTVKEMIQALADARYDDSIGVIVLT
                     GEGEKAFCSGGDQKVRGDYGGYQDDAGTHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGG
                     HVLHMMCDLTIAAENAIFGQTGPKVGSFDGGWGASYMARIVGQKKAREIWFLCRQYNAQ
                     EALDMGLVNTVVPIADLEKETVRWCREMLQNSPMALRCLKAALNADCDGQAGLQELAGN
                     ATMLFYMTEEGQEGRNAFNEK"
     gene            8096..9061
                     /gene="menC"
     CDS             8096..9061
                     /codon_start=1
                     /gene="menC"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1268:mandelate racemase/muconate lactonizing enzyme
                     (Score: 58.6; E-value: 7.9e-18)"
                     /gene_kind="biosynthetic-additional"
                     /note="O-succinylbenzoate synthase"
                     /product="MenC"
                     /protein_id="ACD75095.1"
                     /transl_table=11
                     /translation="MRRAQVYRWQIPMDAGVVLRERRLKTRDGFFVQLQQGEGQGWGEI
                     SPLPGFSLESLEEAQAALMAWVEEWRTGLNPPLPDAPSAAFGISCALAELDGSLPDEAD
                     YRAAPLCTGDPDELFALLSAMPGEKVAKIKVGLYEAVRDGMVANLLLEAIPDLRLRLDA
                     NRAWTPLKAQQFAKYVNPAYRSRIAFLEEPCKTREDSRAFARETGIAIAWDESLREADF
                     AFSAEPGVSAVVIKPTLTGSLAKVREQVAAAHGAGLTAVISSSIESSLGLTQLARIAAW
                     LTPDTIPGLDTLNLMQTQLIRPWPGSALPCAGVEALEPLQ"
     gene            9058..10419
                     /gene="menE"
     CDS             9058..10419
                     /NRPS_PKS="Domain: AMP-binding (8-353). E-value: 2.7e-66.
                     Score: 215.7. Matches aSDomain:
                     nrpspksdomains_menE_AMP-binding.1"
                     /codon_start=1
                     /gene="menE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002:AMP-dependent synthetase and ligase (Score:
                     245.6; E-value: 1.3e-74)"
                     /gene_kind="biosynthetic-additional"
                     /note="O-succinylbenzoate-CoA ligase"
                     /product="MenE"
                     /protein_id="ACD75096.1"
                     /sec_met_domain="AMP-binding (E-value: 3e-60, bitscore:
                     193.5, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSFSDWPWRHWREQRADKPALRLNDEVLSWQQLCTRIDNLAAGFH
                     QQGVEAGDGVLLLAHNHPQTLLAWLALLQCGARILPVNPQLPHPLLEVLLPQMTLRFAL
                     VLDGHYDGLAALSVHAPSGEYRVAWQPERLASMTLTSGSTGLPKAAVHTCGAHLASAEG
                     VLALMPYGDDDDWLLSLPLFHVSGQGILWRWLQAGARLTVREKQPLEQALQGCTHASLV
                     PTQLWRLLNTHQRIALKAVLLGGAAIPVELTQQARAQGISTFCGYGLTEFASTVCAKEA
                     DGEPDVGSALPGREVQIVNGEVWIKAQSMASGYWRDGALLPLTNSEGWFATRDRGELHD
                     GRLTILGRMDNLFFSGGEGIQPESLERIIATHPHISQVFIVPLNDAEFGQRPVAVVECE
                     PGTDITRLPEWVQGKLARFEQPVHWLVLPAELKNGGIKISRQALKQWVNAQLSG"
     aSDomain        9082..10116
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_menE_AMP-binding.1"
                     /evalue="2.70E-66"
                     /label="menE_AMP-binding.1"
                     /locus_tag="menE"
                     /protein_end="353"
                     /protein_start="8"
                     /score="215.7"
                     /specificity="substrate consensus: Orn"
                     /tool="antismash"
                     /translation="RHWREQRADKPALRLNDEVLSWQQLCTRIDNLAAGFHQQGVEAGD
                     GVLLLAHNHPQTLLAWLALLQCGARILPVNPQLPHPLLEVLLPQMTLRFALVLDGHYDG
                     LAALSVHAPSGEYRVAWQPERLASMTLTSGSTGLPKAAVHTCGAHLASAEGVLALMPYG
                     DDDDWLLSLPLFHVSGQGILWRWLQAGARLTVREKQPLEQALQGCTHASLVPTQLWRLL
                     NTHQRIALKAVLLGGAAIPVELTQQARAQGISTFCGYGLTEFASTVCAKEADGEPDVGS
                     ALPGREVQIVNGEVWIKAQSMASGYWRDGALLPLTNSEGWFATRDRGELHDGRLTILGR
                     MDNLF"
     CDS_motif       9466..9513
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_menE_0001"
                     /evalue="1.10E-03"
                     /label="NRPS-A_a3"
                     /locus_tag="menE"
                     /protein_end="152"
                     /protein_start="136"
                     /score="12.1"
                     /tool="antismash"
                     /translation="MTLTSGSTGLPKAAVH"
     CDS             complement(10420..>11029)
                     /codon_start=2
                     /gene="tctA"
                     /note="putative tricarboxylic transport protein"
                     /product="TctA"
                     /protein_id="ACD75097.1"
                     /transl_table=11
                     /translation="MAAPEAANNASACGSFIPMLTLGVPGSGTTAVMMGALTLYNITPG
                     PAMFTEQPDIVWGLIAALLIANVMLLVMNIPLIGLFTRMLTIPLWFLVPAIAAVSAVGV
                     YAVHSTTFDLILMVVLGVFGYILRKMHFPMSPLILGFVLGEMLEQNLRRALSISNGDVA
                     ILWDSNVTKILLVLAIVVVVVPPVLRLLRRQRKPQPDVG"
     gene            complement(10420..>11029)
                     /gene="tctA"
ORIGIN
        1 gatcctgtct gcggtgatga ccgtgatgat gctctctctg gcgggtatcc caatgacgct
       61 gggcttcatc ggtaagttct acgtgctggc tgtcggtgtg caggcgcacc tgtggtggct
      121 gactgcgggc gtggttatcg gctcggcaat cggtctgtac tactatctgc gtgtggccgt
      181 gagcctgtac ctgagcgcgc ctcagcagct caaccgtgac gcaccaggca actggcagta
      241 cagcgccggt ggtattgtgg tgctgatctc cgcgctgctg gtgctgatct tcggtatcta
      301 tccacagccg ctgatcgata tcgtgcagcg agcaatgcca ttaatgtaaa agcaaaaaac
      361 ccgcctcggc gggttttttt atggttttcg ccctctccct gtgggagagg gacggggtga
      421 gggcaccagc ccgcaccaga cttaagccag ctgtttcctg ctaatcaacg gcccgtcaat
      481 cttcttcatt gaacggtcca gcacctcttc aatcaccgac gacaactcgt tcagctcaaa
      541 cttcgccacg tagccgtcgg ccttcacttt acgaatatga tcttcgttgg cgttgcccga
      601 aagcgaagag tggatcacca ccggaatatc tttcagcagc gggtcggttt tgattttgcg
      661 cgtcagcgtg aaaccgtcca tctctggcat ttcgaggtcg gtcagcacca gggcaatttt
      721 atcggtgatc ggcacccctt cagcctgcgc ctgcgcggcc agcacgccaa ttttctccca
      781 tgcgtcttta ccggtgatat gcagctgcgc cgggatctcc atagcctgta atcccttctc
      841 cagcatggag cgcgcgactt tggaatcttc cgccacaatc gccacagcgc caggcttgat
      901 gtggaactta gtggtctgca gattcgtggc gtgcagatcg tggttggccg gcgtgatgtc
      961 atacaggatc tgctcaacgt ccagcaccat cgccagctcg ttggtatcgg tcttttcgtc
     1021 cagacaggcg atactggtga tgtaacgtcc gctgacggcg gtttccgccg cgtgaacctg
     1081 cttccagtca aggcgcatga tgttctcaac cgattccacc gcaaacgcct gcacgctgcg
     1141 ggcatattcg gtgatcagca ggatgttgag ccccgtcgtc ggcttacagc ccgccacggc
     1201 ggccaggtca atcaccggga tcacctgatc gcggatattc accatcccca tcagcgggga
     1261 cttcatccct gcgggtttgg tgaactccgg catcggcaca atttcacgca gcttaaagac
     1321 gttaatgcca aacagttcgg atttattttc attcagagac gtgcccagac ggaacagcaa
     1381 cagctcaaaa cgattcgaca gggtgagatt tgccctgtca tcaatatctt tctggaaatt
     1441 atccattcta ccctcaagtg aaatgctgcc cttttaatcg ttatcggcat cctgagagaa
     1501 aaatgaagga ctaaacgctg accttagtga aatcgttggc cagctcacat gccgggaaca
     1561 cgtcgcggca ctctgtcagc agtttctcac agccccgggc gtcatagcgc gagctgacat
     1621 gggtgacaat caatcttccg acgttcgctt cacgggcaag cgccgccgcc tgacgcgtgg
     1681 agctgtgacc ccggctgttg gctttctcct ccatcgccgt ttcaagcgtg gcttcgtgca
     1741 ccatcacatc cacctctcgc gccagttgca gagccgcagg gcagggcgcg gtatcgccaa
     1801 aaatcgccag ttttttccct ggctgcggtg gggcgagata gtcccggcca ttaatcgtgc
     1861 ggccgtcctc aagcgtgacg gtttcgccct gtttcaggcg ctggaaaagc ggtccgggct
     1921 tcacaccatc ggcaatcagc gcggcggcgt cgagcgcgcc gggcttatca tgcgcttcga
     1981 tgcggtagcc atagcactca accggatggt tgagcggccg ggcggtgacg gtatagtccc
     2041 cgtcatccaa aaccagcccc tcggttatct caatcacttc cagcggataa tccgtccacg
     2101 acccgctcag gcgcagcgtg gtttcaacaa actcagcagt acccgctggc ccgtaaatcg
     2161 tcagcgggtt ggcattaccc gccatcgaac ggctgcacag caggcccggc aggccaaaca
     2221 ggtgatcgcc atgcagatgc gtgataaaga tcttatccag cttaccggga tgataagcgg
     2281 tgtgtaacag ctgatgctgc gttccttcgc cacagtcaaa cagccacagt ccgccacggg
     2341 ttgggtgctg cagatccagc aggatcgcgg tcacgtttcg tgtgcgtgtt ggcacgccag
     2401 cggacgtacc cagaaaaatc agttccataa cgcaatcagc cctttgccga atgggggagg
     2461 gtttagtata acgtgatgaa cccaggaagg agaccgacat gatccagtgg caagatttac
     2521 accatagtga actcaccgtt cagacactgt acgccctgct caaactgcgc tgcgaagtgt
     2581 ttgttgtgga acaaacctgc ccgtatcagg acatcgatgg tgatgatctg gtgggcgaga
     2641 accgtcatat tctcggctgg agggataagg agctggtggc gtatgcgagg attctgaaaa
     2701 gcgaggacga ttttgagcct gtcgttattg gtcgcgtcat tattagcggc aaggcgcgcg
     2761 gtgaaaaact gggctatcag ctgatggaaa aaacgctgga ggcatgccaa aaacagtggc
     2821 cagacaaggc gttatacctt ggcgcacagg cgcaccttca gtctttctac ggccactttg
     2881 gctttatgcc ggtcacggac gtgtacgacg aagacggcat tcctcatatc ggtatggcgc
     2941 gggaagtgaa acaggcgtaa tggcaggtcg ttggctatag ttagcggact ggtgtactaa
     3001 catggagaaa aatatgtcat ttcaatcctg ggatacccgt gtcgatgacg acctgacgtt
     3061 actgagcgaa acgctggaag aggtgttacg ttcgtccggc gaccctgccg atcagaaata
     3121 tatcgaactc aaggcgcgtg ccgaacaggc gctacatgac gtcaaaaccc gcgtcagcca
     3181 ggcgtccgac aattactact accgcgcaaa aaaagcggtg tatcgcgccg atgattacgt
     3241 ccatgaaaaa ccgtggcaag gcattggtgt cggtgcggcg gtgggtctgg tgctggggct
     3301 gcttctggcc cgtcgttaac ccctgaatcg gacgcttaac ccctccctat acgtgggtac
     3361 tgatatttct gatggtaccc cgtatactaa gcgtttttac agggagaggt tcgcgtgcat
     3421 tcgatttcca acgcgctgga acgtctgcgt actcagcttg cgcaagcgtt acccgctaca
     3481 cccggcttac gtcacgttga cgtctctttc ccgttaaacg acgctttcga ccctctcgcc
     3541 tggcttggtg ctcagtgcct gtttccccag ttttactggc agcagcgtaa cggtgatgaa
     3601 gagctggctg cgctgggcgc cgtcatccat ttttcctctc tggctcaggc ttcgcagttt
     3661 ttacagcaac agcctgtagc gaacgacacg cggatctgcg gcctgaatgc cttcaccccg
     3721 gagcagggca gtctcttttt accccgcctg ctgtggcgac gttctgccgg gaccgccacg
     3781 ttgcgtctgc agctgtggag tgaccgttcc cttcaggagg atgccgacgc ggcgctggcg
     3841 ttcttgcagc agcttcgtga tgcccgccca atccgcccgc tctccgccca ggtagtgcag
     3901 gaaacccatc agccgcaaaa accggaatgg ctgcgcctta tccgccaggc gacagagacc
     3961 atcgcgcgcg gcgaagtcga aaaggtggtg ctcgcccggg caaccgatct gcagttcagt
     4021 cagccggtga atgccgtggc gctgatggcg gccagccgtg ccctgaatct gaactgctat
     4081 catttctgca tggttttcga tgccagcaac gcgtttcttg gctccacacc tgaacggctg
     4141 tggcggcggc gcggcaacct gctgcgtacc gaagcgctgg ccggtaccgt cgccagccat
     4201 ccagacgaca agcaggcccg ccttctgggc gactggctga tgaacgatga taaaaaccag
     4261 cgtgaaaaca tgctggtggt agaggatatc tgccagcggc tgcagcacca tacccggacg
     4321 ctggaggtcc tgccagcaca ggttatgcgg ttgcgcaaag tgcagcatct tcgtcgctgc
     4381 atctggaccg agctcaggca tgcggatgat gagcaatgtc tgcatgttct gcagccgacg
     4441 gctgcggttg ccgggttacc gcgccagcca gcacgcgaat tcatacaccg cgttgaacct
     4501 ttcgaccggg agtggtatgc cggatctgcc ggatatctgt caccagcaca aagcgagttc
     4561 tgcgtggccc tgcgctcggc gcgcgtccat gacgccaccg tccgtcttta cgccggggcg
     4621 gggatcgtca gcggttccga tccggaacag gagtggcagg agatcgaaaa taaagccgca
     4681 ggcttgcgct ctctgctcct gaaggattag tactgattcg cgcaatcccg attcatatca
     4741 aaattctatt cttttctatt atttatactg tgtcacattc ttgataccgg acaatctcat
     4801 gtcagtaagt tcttttaacc gacgctgggc gacggtgatc cttgaagccc tgactcgcca
     4861 tggcgtcagg catgtgtgta ttgcgccggg ctcacgctcc acaccgctga cgctggcggc
     4921 agcagagaac cgggcattaa ttcaccacac ccattttgac gagcgcggcc tgggccacct
     4981 ggctctgggg ctggcaaagg tcagcaagga accggtggcg gtgatcgtca cctctggtac
     5041 tgccgtggca aacctctanc cggcccttat tgaagccggg ctgaccggtg agaaactggt
     5101 tctgctgacg gccgatcgcc cgccggagct tattgactgc ggagcgaacc aggctattcg
     5161 tcagccgggc ctctttgcct cgcatccttc gcagacggtt tcgttacccc gacccacgca
     5221 ggacattccc gcaagctggc tggtctcgac cgtcgatcat gccatggaga cgttgcgcag
     5281 cggggcgtta catattaact gtccgtttgc cgagccgttg tacggcgaaa tggacgacac
     5341 cggtcttgcc tggcagcagc agcttggcga ctggtgggag agcgaaaagc cgtggctgcg
     5401 cgagcagaca caccttgaga gcgcaaagca acgtgactgg ttcttctggc gacaaaaacg
     5461 cggcgtggtt attgccgggc gcatgagcgc cgccgaaggc aggcaggtgg ccgaatgggc
     5521 acacaccctt ggctggccgc tgattggcga cgtgctgtcc cagaccggac agccgctgcc
     5581 gtgtgccgat ctctggctcg ggaatgcgaa agcggtgact gagctggcgc aggcgcagat
     5641 tgtggttcag ctgggcgcaa gcctgaccgg caaacggtta ctgcagtggc aggcaacctg
     5701 tagcccggaa gagtactggc tggtggatcc gctggaaggg cggctcgatc cggcgcacca
     5761 tcgcggtcgt cgtctggtga gcgacattga cacctggctg gcgctccacc cggcggaaaa
     5821 gcgcgcccca tgggccgttg agatcccggc gctgtcgcgt caggcgtggg agttgacccg
     5881 tgcacaatgc gaagagtttg gtgaagcgga gctggcgcac cgtattcacc ggtatctgcc
     5941 ggagcagggg caactgtttg tcggcaacag cctggtggtg cgtctgattg atgcgttctc
     6001 aaaactgcct gcgggctatc cggtctacag taaccgggga gccagcggca ttgacgggct
     6061 gatctccacg gcggcgggtg tgcagcgagc cagcgcgaaa tccacgctgg cgatcgtggg
     6121 cgatctttcg gcactatacg atctcaatgc gctggcgctg ctgcggcagg tgtcggcgcc
     6181 gtttgtgctg attgtggtca acaataacgg cgggcagatc ttctccctgc tgccgacacc
     6241 gcagagcgaa cgcgagcgct tctatctgat gccgcagaac gtgcagtttg aacatgccgc
     6301 agcgatgttt aacctgaaat accaccgccc ggaaaactgg gaagcgctgg atgcagccct
     6361 gagcagcgcc tggcgacagc ccggtgcaac gctgattgaa ctggtcgtca acgaggctga
     6421 cggtgcgcag aagctgcaaa gtctgctggc gcaggtgagc cacctgtgat cctcgcaggc
     6481 gcgcaacagg ccggtaagcc aggttacccc tggctggtct ttttgcatgg attttctggc
     6541 gattgccgcg agtggcagac ggtcggcgag gcgctcagcg attatccccg gctttatctc
     6601 gacctgccgg ggcatggccg gtccgccagt atggccgtca cggggttcga cgagatgagt
     6661 gacgtgctta atcgaaccct tgttagttac aacatactta actactggct ggtggggtac
     6721 tcccttggcg gccgcattgc gatgtttcat gcctgccagg atcctgcggg gctttgcggc
     6781 atcatggtgg aaggcggaca tccgggcctg aagggggtcg cagcgcgaga ggcaagagca
     6841 gcatccgacc atctgtgggc gcagcgtttt cgcacgcagc ccctctccaa cgtctttgcc
     6901 gactggtatc aacagcctgt ctttgcctcc ctgacggaca gccagcgcag ggcgctcatc
     6961 gccctgcgct gtcgcaacgc gggcacaaac ctggcgatga tgcttgaggc aacctcgctc
     7021 gccgtgcaac cagacctgcg tgccgccctc agcgcgcgac atttcccttt tgattatctc
     7081 tatggcgaac gtgacgagaa gttcgcggcc ctggccgctg aactgaacgc tgttcgccat
     7141 gtcattccaa acgccggaca caacgcccac cgggaaaatc ccggggcggt tagcgcgagt
     7201 ctggctcaga tactgcgtcg tcgaataaag gacactctat gatctatcct gatgaaaaca
     7261 tgctctatgc accggttgaa tggcaagact gctccgaagg ttacaccgac attcgttacc
     7321 acaaatcggc tgacggtatc gccaaaatca ccattaatcg tccgcaggtg cgtaacgcct
     7381 ttcgtcccct gaccgtaaaa gagatgattc aggccctggc ggatgcgcgc tatgacgaca
     7441 gcatcggcgt tatcgtcctg accggggaag gcgaaaaagc gttctgctcc ggtggagatc
     7501 agaaagtacg cggtgactac ggcggatacc aggacgatgc gggtacccat cacctgaacg
     7561 tgcttgattt ccagcgtcaa atccgcacct gcccaaaacc cgtggtggcc atggtggcag
     7621 gctactccat cggcggcggc cacgtgctgc acatgatgtg cgatctgacc attgccgcag
     7681 agaacgccat cttcggtcag acgggcccga aagtcggctc ttttgacggc ggctgggggg
     7741 cttcttacat ggcgcgcatt gtcggtcaga aaaaagcccg cgaaatctgg ttcctgtgcc
     7801 gtcagtataa cgcccaggaa gcgctggata tgggcctggt taacaccgtg gtgccgattg
     7861 ccgatctcga aaaagagacc gttcgctggt gtcgcgaaat gctgcagaac agcccgatgg
     7921 cgctgcgctg cctgaaagcg gccctgaacg ctgactgtga cggccaggcg gggctgcagg
     7981 agctggcggg taacgccacc atgctgttct acatgaccga agagggtcag gaaggacgca
     8041 acgcgtttaa tgaaaaacgc cagccagact tcagtaaata caaacggaac ccgtaatgcg
     8101 tcgcgcgcag gtttaccgct ggcagatacc gatggacgcg ggcgtggtgc tgcgtgaacg
     8161 gcggttaaaa acccgtgatg gcttcttcgt gcaactgcag cagggcgaag ggcagggctg
     8221 gggcgagatt tcgcctctgc cgggctttag cctggagtcg ctggaggagg cgcaggctgc
     8281 gctgatggca tgggtagagg aatggcgtac aggcctgaat ccgccgctgc ccgatgcccc
     8341 ttctgctgcg tttggcatca gctgcgcgct cgcggagctc gacggcagcc tccctgatga
     8401 ggccgattac cgtgccgcgc cgctctgcac gggcgatccg gacgagctgt ttgccttgct
     8461 cagcgcaatg cccggcgaga aagtggcgaa aatcaaagtc ggtctttacg aagcggtacg
     8521 cgacggcatg gttgctaacc tcttgctgga ggcgatcccc gatctgcgcc tgcgtctgga
     8581 tgccaaccgc gcctggacgc cgcttaaggc gcagcagttc gcgaagtacg tcaacccggc
     8641 gtaccgcagc cgcatcgctt ttctggaaga gccgtgcaaa acccgtgagg attcacgcgc
     8701 ttttgcccga gaaaccggca ttgccatcgc ctgggatgaa agcctgcgtg aggcggactt
     8761 tgcctttagc gccgagcctg gcgtcagcgc cgtggtcata aaaccgacgc taacgggcag
     8821 tctggcgaag gtacgcgaac aggtggcggc agcgcatggc gcagggctga cggcggtgat
     8881 cagctcctcc atcgaatcca gccttgggct gacacagctg gcgcgcattg ccgcctggtt
     8941 aacgcccgac accatcccgg ggctggatac gttaaacctg atgcaaaccc agctcattcg
     9001 cccgtggccg gggagcgcgc tgccgtgcgc gggcgttgag gcgctggagc cgttgcaatg
     9061 agttttagcg actggccgtg gcggcactgg cgcgagcagc gtgctgataa accggctctg
     9121 cgtctcaacg acgaggtgct gagctggcaa cagctctgca cccgcatcga caatctggcc
     9181 gcagggtttc atcagcaggg tgtggaggcg ggtgacggcg tgctgctgct cgcccataat
     9241 cacccgcaga ccctgctggc atggctggcg ctgctccagt gcggcgcgcg aattcttccc
     9301 gttaatcccc agctcccgca tccgctgctg gaggtgttgc ttccgcagat gacgctgcgt
     9361 tttgcgctgg tgctggacgg tcactatgac gggcttgccg cgcttagcgt acatgctccg
     9421 tccggcgaat accgcgtggc atggcagcct gagcggctgg cctcaatgac gctcacctcc
     9481 ggctccaccg ggctgccgaa agcggccgtt catacctgcg gggcgcatct tgccagcgct
     9541 gagggcgtcc tcgcgctgat gccctacggc gacgatgacg actggctgct ttcgctgccg
     9601 ctgtttcatg tctccgggca ggggatctta tggcgctggc tgcaggctgg cgcgcgctta
     9661 accgtacgcg agaagcagcc gctggagcag gccttgcagg ggtgtacgca tgcctcgctg
     9721 gtgccaaccc agctctggcg tctgctcaat acccaccagc ggatcgcgct gaaagcggta
     9781 ctgctgggcg gggccgccat ccccgttgag ttgacgcaac aggcgcgcgc gcagggtatt
     9841 tctacgtttt gtggctatgg cctgacggag tttgcctcta ccgtctgcgc gaaagaggct
     9901 gacggtgagc cggacgtggg cagcgcattg ccgggcaggg aggtgcagat cgttaacggt
     9961 gaagtgtgga tcaaggcgca aagcatggcc tcaggctact ggcgcgacgg cgcgttgctg
    10021 ccgctcacga attcagaggg ctggtttgcc acccgcgatc gcggcgaact gcatgacggt
    10081 cgcctgacga tcctcggtcg gatggataac ctctttttca gcggaggcga gggcattcag
    10141 ccggagagtc ttgagcggat tatcgcgacc catccgcaca tcagccaggt cttcattgtt
    10201 ccgctgaatg acgccgaatt tggtcagcgt ccggtagcgg tggtggagtg tgaaccggga
    10261 acagatatca cccgcttgcc ggaatgggta cagggcaaac tggcgcgatt tgagcagccc
    10321 gtgcactggc tggtactgcc tgcggagtta aaaaatggcg ggattaagat ctcccgccag
    10381 gcgttaaaac agtgggtgaa tgcccagctg tctggttaat tagccgacat cgggctgcgg
    10441 cttacgctga cgacgcagca ggcgcagcac cggcggcacg accaccacca caatagccag
    10501 caccagcagg atcttcgtca cattgctgtc ccacagaatg gcgacatcgc cattgctgat
    10561 ggacagggcg cggcgcaggt tctgttccag catctcaccc agcacaaagc ccagaatcag
    10621 cggcgacatc gggaagtgca ttttacgcaa gatgtagccg aacaccccca gcaccaccat
    10681 cagaatcagg tcgaaggtgg tgctgtgcac cgcatacacc cctaccgccg agacggccgc
    10741 gattgcgggt accaggaacc acagcggaat ggtcagcata cgggtgaaca ggccaatcag
    10801 cgggatgttc atgaccagca acattacgtt ggcaatcagc agtgccgcaa tcagccccca
    10861 gacgatatcc ggctgttcgg tgaacatcgc cggtcctggc gtgatgttat agagcgtcag
    10921 cgcccccatc atcaccgccg tggtaccgga gccgggcacg ccgagcgtca gcatgggaat
    10981 aaacgagcca caggccgagg cattgttagc cgcctcgggt gcggcgacg
//
