LOCUS       BGC0000924              5698 bp    DNA     linear   BCT 14-DEC-2009
DEFINITION  Pseudomonas chlororaphis pyrrolnitrin biosynthetic gene cluster,
            complete sequence.
ACCESSION   BGC0000924
VERSION     BGC0000924
KEYWORDS    .
SOURCE      Pseudomonas chlororaphis
  ORGANISM  Pseudomonas chlororaphis
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
COMMENT     ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2024-10-17 14:26:09
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5698
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..5698
                     /db_xref="taxon:587753"
                     /mol_type="genomic DNA"
                     /organism="Pseudomonas chlororaphis"
     subregion       1..5698
                     /aStool="mibig"
                     /contig_edge="True"
                     /label="other"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..5698
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            137..1753
                     /gene="prnA"
     CDS             137..1753
                     /codon_start=1
                     /gene="prnA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Trp_halogenase"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1119:halogenase (Score: 34.3; E-value: 2.6e-10)"
                     /gene_kind="biosynthetic-additional"
                     /note="catalyzes the chlorination of tryptophan to form
                     7-chlorotryptophan"
                     /product="PrnA"
                     /protein_id="ABW69174.1"
                     /sec_met_domain="Trp_halogenase (E-value: 2.3e-183,
                     bitscore: 598.2, seeds: 23, tool: rule-based-clusters)"
                     /sec_met_domain="DAO (E-value: 3.4e-10, bitscore: 28.1,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNKPIKNIVIVGGGTAGWMAASYLVRALQQQANITLIESAAIPRI
                     GVGEATIPSLQKVFFDFLGIPEREWMPQVNGAFKAAIKFVNWRKSPDHSRDDHFYHLFG
                     NVPNCDGVPLTHYWLRKREQGFRQPMEYACYPQPGALDGKLAPCLADGTRQMSHAWHFD
                     AHLVADFLKRWAVERGVNRVVDEVVEVRLNDRGYISTLLTKEGRTLEADLFIDCSGMRG
                     LLINQALKEPFIDMSDYLLCDSAVASAVPNDDAREGVEPYTSAIAMNSGWTWKIPMLGR
                     FGSGYVFSSKFTSRDQATADFLNLWGLSDNQPLNQIKFRVGRNKRAWVNNCVSIGLSSC
                     FLEPLESTGIYFIYAALYQLVKHFPDTSFDPRLSDAFNAEIVHMFDDCRDFVQAHYFTA
                     SREDTPFWLANRHELRLSDAIKEKVQRYKAGLPLTTTSFDDSTYYETFDYEFKNFWLNG
                     NYYCIFAGLGMLPDRSLPLLQHRPESIEKAEAMFASIQREAERLRTSLPTNYDYLRSLR
                     DGDAGLSRSQPGPTLAAREIL"
     misc_feature    137..5698
                     /note="pyrrolnitrin biosynthetic gene cluster"
     gene            1753..2835
                     /gene="prnB"
     CDS             1753..2835
                     /codon_start=1
                     /gene="prnB"
                     /gene_functions="biosynthetic (rule-based-clusters) other:
                     prnB"
                     /gene_kind="biosynthetic"
                     /note="catalyzes the conversion of 7-chlorotryptophan to
                     monodechloroaminopyrrolnitrin"
                     /product="PrnB"
                     /protein_id="ABW69175.1"
                     /sec_met_domain="prnB (E-value: 4.6e-231, bitscore: 754.0,
                     seeds: 24, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MERTLDRVSAFAATHAAVAACDPLQARSLVLQLPGLNRNKDVPGI
                     VGLLREFLPARGVPSGWGFVEAAAAMRDIGFFLGSLKRHGHEPADVVPGLEPVLLDLAR
                     ATDLPPRETLLHVTVWNPAAADAQRSYTGLPDEAHLLESVRISMATLEAAIAVTVELSE
                     VPLRSPAFAPGCDELAAYLQKMVESIVYAYRFISPQVFYDELRPFYEPIRVGGQSYLGP
                     GAVEMPLFVLEHVLWGSQSDDQVYREFKETYLPYVLPAYRAVYARFAGRPALIDRVLGE
                     ARVGARGEPVRAGLTALERVFKVLLRFRAPHLKLAERAYEVGRSGPTTGSGGYAPSMLG
                     DLLTLTCAARSRIRAVLDES"
     gene            2879..4582
                     /gene="prnC"
     CDS             2879..4582
                     /codon_start=1
                     /gene="prnC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Trp_halogenase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1119:halogenase (Score: 401.7; E-value: 7.6e-122)"
                     /gene_kind="biosynthetic-additional"
                     /note="catalyzes chlorination of
                     monodechloroaminopyrrolnitrin to form aminopyrrolnitrin"
                     /product="PrnC"
                     /protein_id="ABW69176.1"
                     /sec_met_domain="Trp_halogenase (E-value: 5.8e-14,
                     bitscore: 39.8, seeds: 23, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTQKSPANGRDSTHFDVIILGSGMSGTQMGAILAKQQFRVLIIEE
                     SSHPRFTIGESSIPETSLMNRIIADRYGIPELDHITSFYSTQRYVASSTGIKRNFGFVF
                     HKPGQEHDPKEFTQCVIPELPWGPESHYYRQDVDAYLLQAAIKYGCTVHQKTSVSEYHA
                     DKDGVAVTTAQGERFTGRYMIDCGGPRAPLATQFKLREEPCRFKTHSRSLYTHMLGVKP
                     FDDIFKVKGQRWRWHEGTLHHMFTGGWLWVIPFNNHPRSTNNLVSVGLQLDPRVYPKTD
                     IPAQQEFDEFLARFPSIAAQFRDAVPVRDWVKTDRLQFSSNACVGDRYCLMLHANGFID
                     PLFSRGLENTAVTIHALAARLIKALRDDDFSPERFEYIERLQQKLLDHNDDFVSCCYTA
                     FSDFRLWDAFHRLWAVGTILGQFRLVQAHARFRASRDEGDLDHLDNDPPYLGYLCADME
                     EYYQLFNDAKAEVEAVSAGRKPAEEAAARIHALIDERDFAKPMFGFGYCITGAKPQLNN
                     SKYSLLPAMKLMYWTQTRAPAEVKKYFDYNPMFALLKAYITTRIGLAMKK"
     gene            4607..5698
                     /gene="prnD"
     CDS             4607..5698
                     /codon_start=1
                     /gene="prnD"
                     /note="catalyzes the oxidation of aminopyrrolnitrin to form
                     pyrrolnitrin"
                     /product="PrnD"
                     /protein_id="ABW69177.1"
                     /transl_table=11
                     /translation="MNDIQLDQASIKKRPPGAYDATTRLAASWYVAMRSDALKDKPTEL
                     TLFGRPCVAWRGATGRAVVMDRHCSHLGANLADGRVKDGCIQCPFHHWRYDEQGQCVHI
                     PGHNQAVLRLEPVPRGARQPTLVTVERYGYVWVWYGSPQPLHPLPEIAAADVDNGDFMH
                     LHFAFETTTAVLRIVENFYDAQHATPVHALPISAFELKFFDDWRRWPEVESLARAGAWF
                     GAGIDFTVNRYFGPLGMLSRALGLNMSQMNLHFDGYPGGCVMTVALDGNVKYKLLQCVT
                     PVSDGKNVMHMLISIKKVGGALRRATDFVLFGLQTRQAAGYDVKIWNGMKPDGGGAYSK
                     YDKLVLKYRAFYRGWVDRVASEQ"
ORIGIN
        1 acggaatttg cgtcgctttc ccggcaggct agagtctcta ctctaaccag cacattgatg
       61 tgcctcttgc acggattgca cgagattggc ggccttcctc tcgtcacagg cagtccgcct
      121 tgaaacaagg agtgttatga acaagccgat caagaatatc gtcatcgtgg gcggcggcac
      181 tgcgggctgg atggccgcct cgtacctcgt ccgggcgctc cagcagcaag cgaacattac
      241 gctcatcgag tctgcggcga tcccccggat tggcgtgggc gaggcgacca tcccgagttt
      301 gcagaaggtg ttcttcgact tcctcgggat accggagcgg gagtggatgc cccaggtgaa
      361 cggcgcgttc aaggccgcga tcaagttcgt gaattggaga aagtctcccg accactcgcg
      421 tgacgatcac ttctaccatt tgttcggcaa cgtgccgaac tgcgacggcg taccgctgac
      481 ccactactgg ctgcgcaagc gcgaacaggg cttccggcag ccgatggagt acgcgtgcta
      541 cccgcagccc ggggcgctcg acggcaagct ggcaccgtgc ctggccgacg gcacccgcca
      601 gatgtcccac gcgtggcact tcgacgcgca cctggtggcc gacttcttga agcgctgggc
      661 cgtcgagcgc ggggtgaacc gcgtggtcga cgaggtcgtg gaggttcgcc tgaacgaccg
      721 cggctacatc tccaccctgc tcaccaagga ggggcggacg ctggaggcgg acctgttcat
      781 cgactgctcc ggcatgcggg ggctcctgat caatcaggcc ctgaaggagc ccttcatcga
      841 catgtccgac tacctgctgt gcgacagcgc ggtcgccagc gccgtgccca acgacgacgc
      901 gcgggagggg gtcgagccgt acacctccgc gatcgccatg aactcggggt ggacctggaa
      961 gattccgatg ctgggccggt tcggcagcgg ctacgtcttc tcgagcaagt tcacctcgcg
     1021 cgaccaggcc accgccgact tcctcaacct ctggggtctc tcggacaacc agccgctcaa
     1081 ccagatcaag ttccgggtcg ggcgcaacaa gcgggcgtgg gtcaacaact gcgtctcgat
     1141 cgggctgtcg tcgtgctttc tggagcccct ggaatcgacg ggaatctact tcatctacgc
     1201 ggcgctgtac cagctcgtga agcacttccc cgacacctcg ttcgacccgc ggttgagcga
     1261 cgcattcaac gccgagatcg tccacatgtt cgacgactgc cgggatttcg ttcaggcgca
     1321 ctatttcacc gcgtcgcgcg aagacacccc gttctggctg gcgaaccggc acgagctgcg
     1381 gctctcggac gccatcaaag agaaggttca gcgctacaag gcggggctgc cgctgaccac
     1441 cacgtcgttc gacgattcca cgtactacga gaccttcgac tacgaattca agaacttctg
     1501 gttgaacggc aactactact gcatctttgc cggcctgggc atgctgcccg accgctcgct
     1561 gccgctcttg cagcaccgac cggagtcgat cgagaaggcc gaggcgatgt tcgccagcat
     1621 ccagcgcgag gccgagcgtc tgcgcacgag cctgccgacc aactacgact acctgcggtc
     1681 gctgcgtgac ggcgacgcgg ggctgtctcg cagccagccc ggaccgacgc tcgcggcgcg
     1741 ggaaatcctc tagtggaacg caccttggac cgggtatccg cattcgcggc cacgcacgct
     1801 gccgtggcgg cctgcgatcc gctacaggcg cgctcgctcg ttctgcagct gccgggcctg
     1861 aaccgtaaca aggacgtgcc cggcatcgtc ggcctgctgc gcgagttcct cccggcgcgc
     1921 ggcgtgccct ccggctgggg cttcgtcgaa gccgccgcgg cgatgcgcga catcgggttc
     1981 ttcctggggt cgctcaagcg gcacggacat gagcccgcgg acgtggtgcc cgggcttgag
     2041 ccggtgctgc tcgacctggc acgcgcgacc gacctgccgc cgcgcgagac gctcctgcat
     2101 gtgacggtct ggaaccccgc ggcggccgac gcgcagcgga gctacaccgg gctgcccgac
     2161 gaagcgcacc tgctcgagag cgtgcgtatc tcgatggcga ccctcgaggc ggccatcgcg
     2221 gtgaccgtcg aactgtccga ggtgcccctg cgctcgcccg cgttcgcgcc agggtgcgac
     2281 gagctggcag cctatctgca gaaaatggtc gaatcgatcg tctacgcgta ccgcttcatc
     2341 tcgccgcagg tcttctacga tgagctccgc cccttctacg aaccgattcg ggtcgggggc
     2401 cagagctacc tcggccccgg tgccgtagaa atgcccctct tcgtgctgga gcacgtcctg
     2461 tggggctcgc aatcggacga ccaggtttat cgagaattca aggagacgta cctgccctat
     2521 gtgcttcccg cgtacagggc ggtctacgct cggttcgccg ggcggccggc gctcatcgac
     2581 cgcgtgctcg gcgaagcgcg ggtcggtgcg cggggcgagc ccgtccgggc cgggctgaca
     2641 gccctcgagc gggtcttcaa ggtcctgctg cgcttccgcg cgcctcacct caaattggcg
     2701 gagcgggcgt acgaagtcgg gcgaagcggc cccacaaccg gcagcggggg ctacgcgccc
     2761 agcatgctcg gcgatctact cacgctcacg tgtgccgcgc ggtcccgcat ccgcgccgtg
     2821 ctcgacgaat cctgacgcgc acggcccagg tgttatctca caaggagagt ttgcccccat
     2881 gactcagaag agccccgcga acgggcgtga tagcacccat ttcgacgtga tcatcctcgg
     2941 ctcgggcatg tccggcaccc agatgggggc catcctggcc aaacaacagt ttcgcgtgct
     3001 gatcatcgag gagtcgtcgc acccgcggtt cacgatcggc gaatcgtcga tccccgagac
     3061 gtctctcatg aaccgcatca tcgccgatcg ctacggcatt ccggagctcg accacatcac
     3121 gtcgttctac tcgacgcagc gttacgtcgc gtcgagcacg ggcatcaagc gcaacttcgg
     3181 cttcgtgttc cacaagcccg gccaggagca cgacccgaag gagttcacgc agtgcgtcat
     3241 tcccgagctg ccgtgggggc cggagagcca ttattaccgg caagacgtcg acgcctacct
     3301 gttgcaagcc gccatcaaat acggctgcac ggtccaccag aagacgagcg tgagcgaata
     3361 ccacgccgat aaagacggcg tcgcggtgac caccgcccag ggcgaacggt tcaccggccg
     3421 gtacatgatc gactgcggag gaccccgcgc gccgctcgcg acccagttca agctccgcga
     3481 agaaccgtgt cgcttcaaga cgcactcgcg cagcctctac acgcacatgc tcggggtcaa
     3541 gccgttcgac gacatcttca aggtcaaggg gcagcgctgg cgctggcacg aagggacctt
     3601 gcaccacatg ttcacgggcg gctggctctg ggtgattccg ttcaacaacc atccgcggtc
     3661 gaccaacaac ctggtgagcg tcggcctgca gctcgacccg cgtgtctacc cgaaaacgga
     3721 catccccgcg cagcaggaat tcgacgagtt cctcgcgcgg ttcccgagca tcgcggcgca
     3781 gttccgggac gccgtgccgg tgcgcgactg ggtcaagacc gaccgcctgc agttctcgtc
     3841 gaacgcctgc gtcggcgacc gctactgcct gatgctgcac gcgaacgggt tcatcgaccc
     3901 gctcttctcc cgggggctcg agaacaccgc ggtgaccatc cacgcgctcg cggcgcgcct
     3961 catcaaggcg ctacgcgacg acgacttctc ccccgagcgc ttcgagtaca tcgagcgcct
     4021 gcagcaaaag cttttggacc acaacgacga cttcgtcagt tgctgctaca cggcgttctc
     4081 ggacttccgc ctgtgggacg cgttccacag gctgtgggcg gtcggcacaa tcctcgggca
     4141 gttccggctc gtgcaagccc acgcaaggtt ccgcgcgtcg cgcgacgagg gcgacctcga
     4201 tcacctcgac aacgaccccc cgtacctcgg gtacctgtgc gcggacatgg aggagtacta
     4261 ccagttgttc aacgacgcca aggccgaggt cgaggccgtg agcgccgggc gcaagccggc
     4321 cgaggaggcc gcggcgcgga ttcacgccct catcgacgaa cgagacttcg ccaagccgat
     4381 gttcggcttc gggtactgca tcaccggggc caagccgcag ctcaacaact cgaagtacag
     4441 cctgctgccg gcgatgaagc tgatgtactg gacgcaaacc cgcgcgccgg cagaggtgaa
     4501 aaagtacttc gactacaacc cgatgttcgc gctgctcaag gcgtacatca cgacccgcat
     4561 cggcttggcg atgaagaagt agtcggccga cgacgacaga aaaacgatga acgacattca
     4621 attggatcaa gcgagcatca agaagcgtcc cccgggggcg tacgacgcga ctacgcgcct
     4681 ggcggcgagc tggtacgtcg cgatgcgctc ggacgccctc aaggacaagc cgacggagtt
     4741 gacgctcttc ggccgtccct gcgtggcgtg gcgcggagcg acggggcggg ccgtggtgat
     4801 ggaccgccac tgctcgcacc tgggcgcgaa cctggctgac gggcgggtca aggacgggtg
     4861 catccagtgc ccgttccacc actggcggta cgacgagcag ggccagtgcg ttcacatccc
     4921 cggccacaac caggcggtgc tccggctgga gcccgtgccg cgcggggcgc gccagccgac
     4981 gttggtcacc gtcgagcgat acggctacgt gtgggtctgg tacggctccc cgcagccgct
     5041 gcacccgctg cccgaaatcg ccgcggccga cgtcgacaac ggcgacttca tgcacctgca
     5101 cttcgcgttc gagacgacga cggcggtctt gcggatcgtc gagaacttct acgacgcgca
     5161 gcacgcgacc cccgtgcacg cgctcccgat ctcggccttc gagctcaagt tcttcgacga
     5221 ttggcgccgg tggccggagg ttgagtcgct ggcccgggcg ggcgcgtggt tcggtgccgg
     5281 gatcgacttc accgtgaacc ggtacttcgg tcccctcggc atgctgtcgc gcgcgctcgg
     5341 cctgaacatg tcgcagatga acctgcactt cgatggctac cccggcgggt gcgtcatgac
     5401 cgttgccctg gacggaaacg tcaaatacaa gctgctccag tgcgtgacgc cggtgagcga
     5461 cggcaagaac gtcatgcaca tgctcatctc gatcaagaag gtgggcggcg ccctgcgccg
     5521 cgcgaccgac ttcgtgctgt tcgggctgca gaccaggcag gcagcggggt acgacgtcaa
     5581 aatctggaac gggatgaagc cggacggcgg aggcgcgtac agcaagtacg acaagctcgt
     5641 gctcaagtac cgtgcgttct accggggctg ggtcgaccgc gtcgcgagtg agcagtga
//
