LOCUS       BGC0000926             12724 bp    DNA     linear   BCT 22-MAR-2010
DEFINITION  Bacillus subtilis subsp. spizizenii ATCC 6633 putative
            phosphoglucosamine mutase, putative glucosamine-fructose-6-phosphate
            aminotransferase, putative NADH dehydrogenase subunit 5,
            hypothetical transmembrane protein, hypothetical proteins, putative
            two-component response regulator ybdJ, and putative sensor histidine
            kinase genes, complete cds; rhizocticin biosynthetic gene cluster,
            complete sequence; and putative serine-threonine kinase,
            hypothetical proteins, putative amino acid permease, putative
            sigma-G-dependent sporulation-specific SASP protein, hypothetical
            protein, putative beta-lactamase, putative cytochrome P450, and
            hypothetical protein genes, complete cds.
ACCESSION   BGC0000926
VERSION     BGC0000926
KEYWORDS    .
SOURCE      Bacillus subtilis subsp. spizizenii ATCC 6633 = JCM 2499
  ORGANISM  Bacillus subtilis subsp. spizizenii ATCC 6633 = JCM 2499
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 14:26:29
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 12724
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..12724
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[1046:11466]"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1047..11466
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
     cand_cluster    1..12724
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /kind="single"
                     /product="phosphonate"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..12724
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000926"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..12724
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="phosphonate"
                     /region_number="1"
                     /rules="(phosphonates and minimum(1, [2-Hacid_dh_C, Aldedh,
                     Aminotran_1_2, Aminotran_5, AurF, CTP_transf_like, DUF4992,
                     Fe-ADH, HMGL-like, Metallophos_2, NTP_transf_3,
                     NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C, TPP_enzyme_N,
                     phosphonates]) and not (phosphonatesHydrolase or
                     phosphonatesMeIsocitrateLyase))"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            complement(2..892)
                     /gene="rhiA"
     CDS             complement(2..892)
                     /codon_start=1
                     /gene="rhiA"
                     /gene_functions="regulatory (smcogs) SMCOG1014:LysR family
                     transcriptional regulator (Score: 167.8; E-value: 5.4e-51)"
                     /gene_kind="regulatory"
                     /note="RhiA"
                     /product="putative transcriptional regulator"
                     /protein_id="ADB43066.1"
                     /transl_table=11
                     /translation="MNLHALRLFYTVAEEGNVTCAAKKLNISQPAVTAQIKKLEKEIGL
                     TLLSPRGRGIYLTQTGLQLAKQAKRLFSLQNEIESYLEHLKEGSVGKLHIVATSLPANF
                     LLPKWISRFKGEYPDVDVEITTVNSTEAISQLVNYKADIGIIGGNRTFNKHISSTLLLE
                     DEMVFISNNKHKYAEQTTTLAEIVKEPFVFREEGSFSRELLFSLCNLFNVNKPCTGLQM
                     NGLNETINTVIEGYGITFVSFLEVNRYISRGDVKRINVEHVDVTNPISLCWRNDDVIST
                     SAFNFIELIRKEKME"
     misc_feature    2..12724
                     /note="rhizocticin biosynthetic gene cluster"
     gene            1047..2348
                     /gene="rhiB"
     CDS             1047..2348
                     /codon_start=1
                     /gene="rhiB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: PALP"
                     /gene_kind="biosynthetic"
                     /note="RhiB"
                     /product="putative threonine synthase"
                     /protein_id="ADB43067.1"
                     /sec_met_domain="PALP (E-value: 3.3e-57, bitscore: 184.0,
                     seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLVCIDCGQEMSSDDFKYQCSCGGLVDIVHDFSHYDTDHLKHVF
                     HERLSERMSVFASGVWRYKELIYPDLPVESIVTKYEGNTGLYRSEMLSKYTGVRKVYLK
                     AQSENPSGSFKDNGMTVAVSHGKHLGYEKYACTSTGNTSSSLGMYASIANASSYVFVPD
                     KEISINKVLQTAAYGGNVFSIPGTYDDGIRFLEKYSGDFGFYICNSINPFRIEGQKSII
                     YEIAQYLDWKLPDWIIVPGGALSNATALGKGLRDLYALNFIDKIPRVAIIQAEGASPFH
                     QMISKNKETIDPEPFPYTRASALNIGNPPSWKKARKVLEDTNGITVSVTDEDILDAKAI
                     IDRTGVGCEPASASTAAGLRQLRSQQVIDKDESVLCILTGNILKDTGALHDYHFGKDLN
                     GKFKNNIKSAELSFGKIKSAMETIRDLNGLLKIH"
     gene            2385..3611
                     /gene="rhiC"
     CDS             2385..3611
                     /codon_start=1
                     /gene="rhiC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp"
                     /gene_kind="biosynthetic-additional"
                     /note="RhiC"
                     /product="putative amino acid ligase of ATP-grasp
                     superfamily"
                     /protein_id="ADB43068.1"
                     /sec_met_domain="ATP-grasp_3 (E-value: 1.3e-08, bitscore:
                     25.0, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="ATP-grasp (E-value: 4.3e-09, bitscore:
                     26.0, seeds: 20, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSILILNKTSYSKSPYDLWLKDLEEPVVMLTSTERLHECKNYDQI
                     ESFDEYPVNGCIEIRALELHETYSFNTIIAMSEYDLLRAGKLRTHLGLKGQSYESALLF
                     RDKVLMKQRLEEQGIPVPHYRKIESPVDLYLFVQQFGFPVVVKPIDGSGSVDTKVLKNE
                     KDMMKYLSKGLDGNVEVETFVDGDMYHIDGLMIDGHITLNWPSRYINGCLAFQEEQFLA
                     SYQLGADNPLTSRLIGIVEKALKALPSPKTTTFHAEVFHTPDDKLVFCEIASRTGGGLI
                     REAIQQGFGFDLNEVCVKKQCGLPYDIPDYSQLKIGPKQLGGWILIPPKYGRLVKIPSI
                     PFENWVTKQKVSAREGEVFQGASSSVDVVSSYLIKGNSEDVLINRIHQVASWCSENMIW
                     EGKSTCSLK"
     gene            3596..4819
                     /gene="rhiD"
     CDS             3596..4819
                     /codon_start=1
                     /gene="rhiD"
                     /gene_functions="transport (smcogs) SMCOG1202:major
                     facilitator transporter (Score: 278.6; E-value: 9.7e-85)"
                     /gene_kind="transport"
                     /note="RhiD"
                     /product="putative permease of major facilitator
                     superfamily"
                     /protein_id="ADB43069.1"
                     /transl_table=11
                     /translation="MFSQIKEFFGLHPVIKFLLLGTLVTKAAKSMTTPFLAIYLHQETG
                     EGFGVIGLVIGLGYFATTIGGIIGGTLSDRIGRKSVMLCSIFIWSVVFFLFGIVHTILW
                     FSLLNILSGLCHSFFEPVSKALMVELADRKKRLSIFSLRYLAINIGGALGPLLGTYLGL
                     VSSGTPFIITGSVYLCYALLLFFMMNKLLLQNKSNSKKEFHLPFIFKTLKKDIALRFYI
                     AGGIFLLFSYSQMESTLLQYLNLDFVNGVKIFSTLITINAVTVIILQLPLTRLFERYKS
                     MTTISAGSVCCIIGNIGFALSNGWLTFCISMFILTVGEILCFPSMNVLMDELAPDSMRG
                     TYYGAQNFYNVGEFIGPWLGGIILSLFGGTTIFLVAALSICFALTAYRIGNRKHRFVQL
                     YEAEVMNK"
     gene            4855..5358
                     /gene="rhiE"
     CDS             4855..5358
                     /codon_start=1
                     /gene="rhiE"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_N"
                     /gene_kind="biosynthetic"
                     /note="RhiE"
                     /product="putative phosphonopyruvate decarboxylase alpha
                     subunit"
                     /protein_id="ADB43070.1"
                     /sec_met_domain="TPP_enzyme_N (E-value: 4.2e-10, bitscore:
                     29.4, seeds: 75, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNSTAERMLSTLTENNYTHFTGVPCSLLKGFFRLLESKEAIEKQN
                     ITFIPSIREDSALGVASGMYLGGRKCVMLMQNSGLGYCLNVLTSFNFIYDIPILLLISW
                     RGAYGNDAVEHDIIGEKLTDLLDSVDIPYKELDYENSEGTILDALFLIEKTNRPVAILI
                     KDEI"
     gene            5370..5930
                     /gene="rhiF"
     CDS             5370..5930
                     /codon_start=1
                     /gene="rhiF"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_C"
                     /gene_kind="biosynthetic"
                     /note="RhiF"
                     /product="putative phosphonopyruvate decarboxylase beta
                     subunit"
                     /protein_id="ADB43071.1"
                     /sec_met_domain="TPP_enzyme_C (E-value: 4.4e-16, bitscore:
                     48.9, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNKHDAIQLILGQFPSAYLVSTCGHISRDLYNINDRARNFYMVGS
                     MGMAAPVGLGLSTVYPDVPLVVLDGDGSFLMNMGIITMIGHQKPKNFIHVVLDNGMHES
                     TGGQRTVPLVNVTDIALQVGYEYAIEINSGQKSFDLPNEGPGLIHIKVEPRSEKIGKRV
                     HWTPQEIVQRFTNELTLENEVSV"
     gene            5927..6940
                     /gene="rhiG"
     CDS             5927..6940
                     /codon_start=1
                     /gene="rhiG"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: HMGL-like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1271:2-isopropylmalate synthase (Score: 130.6;
                     E-value: 1.3e-39)"
                     /gene_kind="biosynthetic"
                     /note="RhiG"
                     /product="putative aldolase class II"
                     /protein_id="ADB43072.1"
                     /sec_met_domain="HMGL-like (E-value: 4.8e-40, bitscore:
                     127.6, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKMQLIDCTLRDGGNFNNWFFSAEDINQIILHLDKANVNIIEIGY
                     IGGSGSNKEKSVGSLANCTPELLEQLPKTIHSHLAAMVVPSVCELHLLDNLNPQLIPWI
                     RVASYPHNAEDALPYIEYLKNRGFHVSFNVMAASYIEEKDMAELAKKSHVFGADVFYMA
                     DSFGNLTPDGVRKRVAAAFDQADCPVGFHGHNNLGLAFTNALEAMDAGATFIDTSLCGM
                     ARGAGNLPTEQFVSAIHHWDKFGDIPYKLHPILTAAEYVLSSILSSPMKISTPEIISGI
                     SNIHYYFYDLVISKCREVNLDPLQVSTLLGQTLPKRVDMSYIESVIDQNFRQESAK"
     gene            6937..7827
                     /gene="rhiH"
     CDS             6937..7827
                     /codon_start=1
                     /gene="rhiH"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: phosphonates"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: PEP_mutase"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) phosphonates-like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1231:carboxyvinyl-carboxyphosphonate (Score: 291;
                     E-value: 1.6e-88)"
                     /gene_kind="biosynthetic"
                     /note="RhiH"
                     /product="phosphoenolpyruvate phosphomutase"
                     /protein_id="ADB43073.1"
                     /sec_met_domain="phosphonates (E-value: 1.4e-136, bitscore:
                     444.1, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="phosphonates-like (E-value: 2.8e-145,
                     bitscore: 472.3, seeds: 13, tool: rule-based-clusters)"
                     /sec_met_domain="PEP_mutase (E-value: 5.4e-72, bitscore:
                     232.0, seeds: 69, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKAKKLRELLYSNQVVRVMGAHNGLSAKLAEQAGFHAIWASGLEI
                     SASYAVPDANILTMTENLQAAVVMNESTSIPIICDCDSGYGSVNNVIRMVKEYERNGIA
                     GICIEDKQFPKLNSFVKGSQKLADIDEFSNKIRAAKDVQKNPDFVVIARIEALIAGQGM
                     DEALNRAYAYEAAGADAILIHSKENQPNEIKEFVKQFTGAVPIVIVPTTYPHITVKEME
                     LLGINMVIYANHGLRSSIKAMQETFSQILLDGNTVGVEDNIVSMKTVFELQGMYDMRKQ
                     EDMYNSGTSVISTIK"
     gene            7850..8938
                     /gene="rhiI"
     CDS             7850..8938
                     /codon_start=1
                     /gene="rhiI"
                     /note="RhiI"
                     /product="hypothetical protein"
                     /protein_id="ADB43074.1"
                     /transl_table=11
                     /translation="MISDQYNIVSLAYLHSKINFFIEPSLSLDHVFDFFSTHFFINRGR
                     HEEDRLADVYMTHNKEEFQTIDFNNGEDIYIRKSATDYFTIPCKRVTVEGIEYVKCTKT
                     DTILSFDKENKKIIISTQLQNAEQDELVLIEFIRDLVLKNEENHGVAVVHATSALKDDK
                     ATLIIGSKGKGKSTLLLELVSKHGYKLISGDKTFLWMEDGKLRASGWPDYPHLGLGTLS
                     KYPEFVEHFGLSNKIESVKENLWSTEHKMAIDPVFFKKVIPFAEPGTSFMVECMIYPDL
                     FPSTECGIKQVSNHGELIVPHIERIFTKGNTMWNHYIRPVHQNELERKINQCIELAGKL
                     SAFEVSGSGILEGSVFSERYAL"
     gene            8974..9372
                     /gene="rhiN"
     CDS             8974..9372
                     /codon_start=1
                     /gene="rhiN"
                     /note="RhiN"
                     /product="hypothetical YjbQ-like protein"
                     /protein_id="ADB43075.1"
                     /transl_table=11
                     /translation="MVEITIQSKGFNHSYDITEQVTDALSQLEPSSGLAKVCVTGSTVG
                     LTVMRYEPGTIHDLLQCLESIASKNKQYEHFNTTGDPNGFSHVWSSMIGTSVMVPYKNH
                     KLACSDSHRVVLFDFDLREAERKIYVDR"
     gene            9372..10553
                     /gene="rhiJ"
     CDS             9372..10553
                     /NRPS_PKS="Domain: Aminotran_5 (17-333). E-value: 2.5e-41.
                     Score: 133.7. Matches aSDomain:
                     nrpspksdomains_rhiJ_Aminotran_5.1"
                     /codon_start=1
                     /gene="rhiJ"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139:aminotransferase class V (Score: 81.5; E-value:
                     9.4e-25)"
                     /gene_kind="biosynthetic"
                     /note="RhiJ"
                     /product="hypothetical aminotransferase"
                     /protein_id="ADB43076.1"
                     /sec_met_domain="Aminotran_5 (E-value: 6.7e-31, bitscore:
                     97.3, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNILGELFEMRKLRMATPGPTQVPQEILLAGAQEMIHHRSRQMED
                     IINEINRELPDLFLTKQDIYILLSSGTGAMEAAVSNSFNRGDKVLVVSNGYFGERFTDI
                     CTVFGLEVITVSSPWGTSVNIEEVERAYKQHPDIKGVLTVYSETSTGAVNDVKAIGSLF
                     RDTEVLVIVDAISGLISHELSMDDWGLDIVLAASHKGFMMPPGLAFVAISPKAWKVINE
                     IQPSTYYFSFKRFKKFYPMAPSSPGVSLLLALKKSLDLLKAEGLSNATRRHARIARATQ
                     RGLEALGFHLFVQSPHVRSHTVTAALAPSGIDAALLLKKLNKEYGLTITGGQGEFKGKM
                     IRIGHIGAIDEIDLFGIFGIMELVLTEMGYQVQSGISMTALSDALGGGVIHHA"
     aSDomain        9423..10370
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_rhiJ_Aminotran_5.1"
                     /evalue="2.50E-41"
                     /label="rhiJ_Aminotran_5.1"
                     /locus_tag="rhiJ"
                     /protein_end="333"
                     /protein_start="17"
                     /score="133.7"
                     /tool="antismash"
                     /translation="PGPTQVPQEILLAGAQEMIHHRSRQMEDIINEINRELPDLFLTKQ
                     DIYILLSSGTGAMEAAVSNSFNRGDKVLVVSNGYFGERFTDICTVFGLEVITVSSPWGT
                     SVNIEEVERAYKQHPDIKGVLTVYSETSTGAVNDVKAIGSLFRDTEVLVIVDAISGLIS
                     HELSMDDWGLDIVLAASHKGFMMPPGLAFVAISPKAWKVINEIQPSTYYFSFKRFKKFY
                     PMAPSSPGVSLLLALKKSLDLLKAEGLSNATRRHARIARATQRGLEALGFHLFVQSPHV
                     RSHTVTAALAPSGIDAALLLKKLNKEYGLTITGGQ"
     gene            10546..10803
                     /gene="rhiK"
     CDS             10546..10803
                     /codon_start=1
                     /gene="rhiK"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) GST_N_3"
                     /gene_kind="biosynthetic-additional"
                     /note="RhiK"
                     /product="putative glutaredoxin"
                     /protein_id="ADB43077.1"
                     /sec_met_domain="GST_N_3 (E-value: 9.4e-10, bitscore: 28.8,
                     seeds: 52, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLKLYQREPCPYCKPVREKLTELNVTYINVNLPKDRALRTELIEK
                     TGVPYIPALIDEEDNVIISGKLEYNQHVLDYIEKKFGQNR"
     gene            10819..11466
                     /gene="rhiL"
     CDS             10819..11466
                     /codon_start=1
                     /gene="rhiL"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: Metallophos_2"
                     /gene_kind="biosynthetic"
                     /note="RhiL"
                     /product="hypothetical protein"
                     /protein_id="ADB43078.1"
                     /sec_met_domain="Metallophos_2 (E-value: 2.9e-14, bitscore:
                     43.3, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRYAFITDIHGKRDNLEKVLEHIQTFSSIHEIICLGDVFEVKVPK
                     KDLKDFQFQCMEQVLDLDWELMNLLKDIRVIKGNQEERILSLIPEKEFPKDFYNFLIHM
                     PLEIHLTPMVRLTHGHTFDWTGEEDYWRPSNVHYWSSPWIFYGHNHQNVLFEVKEIENN
                     PYYERKSIINGKPITLDSASRYLINIGDMKYDHPSWMLLDTLENQITYNILY"
     gene            11483..12724
                     /gene="rhiM"
     CDS             11483..12724
                     /codon_start=1
                     /gene="rhiM"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp"
                     /gene_kind="biosynthetic-additional"
                     /note="RhiM"
                     /product="putative amino acid ligase of ATP-grasp
                     superfamily"
                     /protein_id="ADB43079.1"
                     /sec_met_domain="ATP-grasp (E-value: 4.2e-08, bitscore:
                     22.8, seeds: 20, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLRILLINSDKPEPIQFFQKDKETNDSINISVITRSCYAPLYSHW
                     ADHVYIVDDVTDLTVMKSLMLEILKVGPIDHIVSTTEKSILTGGFLRSYFGIAGPGFET
                     ALYMTNKLAMKTKLKMEGIPVADFLCVSQVEDIPAAGEKLGWPIIVKPALGSGALNTFI
                     IHSLDHYEDLYSTSGGLGELKKNNSLMIAEKCIEMEEFHCDTLYADGEILFVSISKYTV
                     PLLKGMAKIQGSFILSQNDPVYAEILELQKSVAQAFRITDGPGHLEIYRTHSGELIVGE
                     IAMRIGGGGISRMIEKKFNISLWESSLNISVYRDPNLTVNPIEGTVGYFSLPCRNGTIK
                     EFTPIEEWEKLAGILEVELLYQEGDVVDEKQSSSFDLARLYFCLENENEVQHLLALVKQ
                     TYYLHLTEDHMMNQ"
ORIGIN
        1 attattccat tttttctttt cttatcagct caataaaatt aaatgccgat gtcgagatga
       61 catcatcatt tctccagcat aacgaaatag ggttagtaac atccacatgt tccacattaa
      121 ttcttttaac atctcctctt gatatatagc gattgacttc aaggaaggaa acaaatgtta
      181 taccataccc ttctatgaca gtattaatgg tctcatttag cccgttcatt tgtaatcctg
      241 tgcatggctt attcacattg aacagattgc ataaagagaa caataattct ctagaaaaac
      301 tcccttcctc gcgaaaaaca aacggttctt ttacaatttc tgctaatgtg gtggtttgtt
      361 ctgcatattt gtgtttgtta ttggagataa ataccatttc atcctccaat aataatgtac
      421 tgctaatatg tttattaaat gtcctgtttc cgccaataat accgatgtca gctttataat
      481 ttactagttg acttatggct tctgttgaat tgactgtggt aatttccaca tcaacatccg
      541 ggtactctcc tttaaaacgg ctgatccact tcgggagtaa aaaattagct ggtaaactag
      601 ttgccacgat gtgcaacttt cccacagagc cttcttttag atgttccagg taggattcaa
      661 tctcattttg gagcgagaat aatctctttg cttgtttagc tagctggagc cctgtttgcg
      721 ttaaataaat tcctcttcct ctagggctaa gcaaagttag tccaatttct ttctctagtt
      781 ttttaatctg agctgtcaca gcaggctgac ttatatttag tttttttgcg gcgcatgtga
      841 catttccctc ctcagctaca gtgtagaaca gtctcaatgc atgaagattc atcgttcatc
      901 tctccattca taaaaaataa ttatgattac aaaaaatata tatattagaa tattccattt
      961 agctatatta ttataaattc acatataaaa cttcaataaa tttctatttt caggtggtga
     1021 ggggacagct tgatgtctag cgaaatatga aacttgtatg tattgactgt ggacaggaaa
     1081 tgtcttctga tgattttaag tatcaatgtt cttgtggtgg actggttgat attgttcatg
     1141 atttttctca ttacgatacc gaccatttaa agcatgtatt tcatgaacgt ctatctgaac
     1201 gtatgtctgt ttttgcaagc ggagtatgga gatacaaaga gcttatatat ccagatttgc
     1261 cagttgagag tattgttaca aaatatgaag ggaacacagg gttataccgc tctgagatgc
     1321 tatcgaaata taccggagtg agaaaggtat atctaaaggc gcaaagtgaa aaccccagcg
     1381 gttcgtttaa ggataacggc atgactgttg ctgtttcaca tggaaaacat ttaggttatg
     1441 aaaaatacgc ctgtacttct accggaaaca cttcttcatc attgggaatg tatgcttcta
     1501 ttgcaaatgc ttcatcctac gtttttgtac cggataaaga aatttcaatt aataaagtat
     1561 tgcagacagc agcttacggt ggaaacgtat tcagtattcc gggaacttat gatgacggta
     1621 tcaggttttt ggaaaagtat agtggtgatt ttggttttta tatatgcaat tctattaacc
     1681 ctttcagaat tgaaggccaa aagagcatta tttatgagat agctcaatat ctagactgga
     1741 aattgcctga ttggattatt gttcctggcg gtgctcttag caatgcaacg gctttaggga
     1801 aaggactccg cgatttatat gctttaaact tcatagacaa gatacctcgg gtagctatca
     1861 tacaagcgga aggcgccagt ccatttcatc aaatgattag taaaaataaa gaaacaattg
     1921 acccggaacc atttccgtac actagagctt ctgcattgaa tataggaaat cctcctagct
     1981 ggaaaaaagc gagaaaggtt ttggaagata caaatggaat cactgtttct gttacagatg
     2041 aggacattct tgatgcaaaa gcaataatcg acagaacagg agtgggctgt gagcctgcct
     2101 ctgcctctac tgcagcagga ctccgtcaat tgaggtcaca gcaggtaata gacaaagatg
     2161 aatctgtttt gtgtatttta acaggcaata tattaaaaga tactggggct ctccacgatt
     2221 accactttgg aaaagatttg aacggcaagt ttaaaaacaa tattaaatct gccgagctat
     2281 catttggaaa gataaaatca gcgatggaga cgattcgtga tttgaatggt ttattaaaga
     2341 ttcattaatt aatcaaaaga cagtacaaga gaggaggaaa agcgattagc attttaatac
     2401 tgaacaaaac ctcatattct aaatctccgt atgatttatg gctaaaggat ttagaagaac
     2461 ctgttgtaat gctgacttca actgaacggt tgcacgaatg caaaaactat gatcagatcg
     2521 agtcatttga tgagtatccg gttaatggat gtattgaaat cagagcgctg gaattacatg
     2581 aaacatatag ttttaacacc ataattgcaa tgtctgagta tgatctttta agagcaggta
     2641 aacttcgtac tcatttagga ttgaaagggc agtcttatga gagtgcacta ttgtttcggg
     2701 acaaagtttt aatgaaacag cgtttggaag aacaggggat tcctgttccc cattaccgga
     2761 aaattgaatc cccggttgac ctgtaccttt ttgtacagca gtttggtttt ccggtagtag
     2821 tcaagccaat agatgggtcg ggatctgtcg atacaaaggt gctaaaaaat gaaaaagaca
     2881 tgatgaaata cttatctaag ggtcttgatg gaaatgttga agtggaaacg tttgtagatg
     2941 gtgatatgta ccatattgat gggcttatga tagacggaca tattacatta aattggccct
     3001 ctcggtatat aaacggatgt ttggctttcc aagaggaaca atttttagcc agttatcagc
     3061 ttggcgctga caacccattg acctccagat taattggcat tgttgaaaaa gcactaaagg
     3121 cgctgccatc cccaaaaaca actacttttc acgcggaagt gtttcatact ccggatgata
     3181 agctagtgtt ttgtgagatt gctagccgga ctggaggggg gttaatacgt gaggctattc
     3241 agcaaggatt tggttttgac ttgaatgagg tttgtgtgaa aaaacagtgc ggcctgccct
     3301 atgatattcc agattattca cagcttaaaa tagggccaaa acagttaggc gggtggattt
     3361 tgatccctcc taaatacggg cggttggtca aaattccttc aattcctttt gagaattggg
     3421 taacaaagca aaaagtgtct gctagagaag gagaggtgtt tcagggtgct tcttctagtg
     3481 tagacgttgt atcaagttac ttaattaaag ggaattcgga agatgtactg atcaatagaa
     3541 ttcatcaagt tgcctcatgg tgcagtgaaa atatgatttg ggaaggcaaa tcaacgtgtt
     3601 ctctcaaata aaagagtttt tcggattaca tccggtcatt aagtttttgc ttttgggaac
     3661 tcttgtcaca aaagcagcaa aatcaatgac aacaccgttt ttggctattt acctccacca
     3721 agaaacagga gagggtttcg gtgtaattgg cttggtaatt ggcttggggt attttgcgac
     3781 tacgattgga ggaatcattg gaggaaccct ttccgacaga atcggcagaa aaagtgttat
     3841 gttatgttcc atttttattt ggagtgttgt tttctttctt tttggcatcg ttcatacaat
     3901 attatggttc agtttgttaa atattttaag cggtctgtgt cattcctttt tcgagccagt
     3961 ttcaaaagct ttaatggttg aattagcgga tagaaagaag aggctcagca ttttttcttt
     4021 acggtatctg gccattaata ttggcggtgc tctaggtccg ctattaggaa catatctggg
     4081 actggtgagc agcggaaccc cttttattat tactggttct gtatatcttt gttatgctct
     4141 tttgttattt tttatgatga ataaactgct tctgcaaaat aaaagcaatt caaaaaaaga
     4201 atttcatttg ccatttatat ttaagacact gaaaaaggat attgctcttc gtttttatat
     4261 agcaggcggg atattcttgt tatttagtta ttctcagatg gagagtacct tgcttcagta
     4321 cctgaacctt gatttcgtga acggcgtgaa gatattctca actttaataa ccataaatgc
     4381 tgtaactgtc attatattgc agttgccatt aacacgcctt tttgaaagat ataaatcaat
     4441 gacaacgatt tctgcgggaa gcgtgtgttg tatcatcggg aatattggtt ttgctttatc
     4501 aaacggttgg cttactttct gtatttctat gtttatatta actgtgggcg aaatcctatg
     4561 ttttccatca atgaatgtat taatggatga gttggcccct gatagtatga ggggaactta
     4621 ttatggagcg cagaattttt ataatgtagg ggagtttata ggcccgtggc ttggaggcat
     4681 tatcctcagc ctttttggcg gtacaaccat tttcttagtt gcagctttgt ctatctgttt
     4741 tgctttaaca gcatatcgta tcggcaacag aaagcataga ttcgttcaac tttatgaagc
     4801 tgaagttatg aataaataat tattaagtta ttttttataa ggggagaatt tgatttgaac
     4861 tcaactgcag aaagaatgct gagtacactt acagaaaaca actatacaca ttttaccgga
     4921 gtgccatgtt cattattaaa aggttttttt aggctgctgg aatcaaaaga ggctatagaa
     4981 aaacaaaata ttacatttat cccctccatt agagaagatt ctgctttagg cgttgcatcc
     5041 ggtatgtatc taggcgggag aaaatgcgtg atgctgatgc aaaattcagg cttaggatac
     5101 tgcttaaatg ttcttacgtc ctttaacttc atatatgata ttcctatttt attactaatt
     5161 agctggagag gggcatacgg taatgatgct gttgaacatg acataatcgg tgaaaaactc
     5221 actgatttac tcgattcagt ggacattcct tacaaagagc tggattatga aaattctgaa
     5281 ggaacgatat tagatgcgct ttttttaatc gagaagacaa accgtcctgt tgcgatatta
     5341 attaaagatg aaatatagga ggtgtcagtt tgaataaaca tgacgcaatt cagcttattt
     5401 taggacagtt tccatcagca tacttggtta gtacatgcgg tcatataagc cgtgatttgt
     5461 acaacataaa tgacagagcc agaaattttt acatggttgg gtccatgggg atggccgctc
     5521 ctgtcggatt gggattgtct accgtgtatc ctgatgttcc tctggttgtg ctagatggag
     5581 atggatcgtt tttaatgaat atggggatta ttacgatgat cggtcatcaa aaacctaaaa
     5641 attttataca tgttgttttg gataacggta tgcatgaaag tacgggcgga caaaggacgg
     5701 ttcctctagt gaatgtaaca gatatagctt tacaagttgg gtatgagtat gccattgaaa
     5761 tcaattcagg gcagaaaagt tttgatttgc ctaacgaggg tccgggactc atccatatta
     5821 aagttgaacc acgaagcgaa aaaatcggca aaagagtaca ttggacaccg caagaaatag
     5881 tccagcgttt tacaaatgaa ttaactttag aaaatgaggt ttcagtatga aaatgcaatt
     5941 aatagattgt acacttcggg acggcggtaa tttcaataac tggttttttt cagctgaaga
     6001 catcaatcaa attatcctgc atctagataa agccaatgta aatataatag aaatcggtta
     6061 tataggaggt tcaggtagca ataaagaaaa atctgtcggg agtttagcca actgtacacc
     6121 tgaactttta gaacagctgc cgaagacgat ccattcacat ttagcggcga tggttgttcc
     6181 atctgtttgt gagcttcatc tattggacaa cctgaatccg cagctgattc catggattcg
     6241 cgttgcttcg tatcctcata atgccgaaga tgctttaccg tatattgaat acctaaaaaa
     6301 tcgaggtttt cacgttagtt ttaatgtaat ggcagccagt tatattgaag agaaagacat
     6361 ggctgagcta gcgaaaaaat cgcatgtttt tggtgcggat gtgttttata tggctgattc
     6421 tttcgggaat ctaacgcctg atggtgttag aaaaagggta gcagctgcat ttgaccaggc
     6481 ggattgccca gttggttttc acggacataa caatcttgga ttagccttta caaatgctct
     6541 tgaagcaatg gatgcgggag caacttttat agatacttca ttatgtggaa tggctcgagg
     6601 agcaggaaac cttccaacag aacaatttgt aagtgcaata catcattggg acaagttcgg
     6661 cgacatccca tacaaactac atccaatttt aactgcggct gaatatgtac tatcttccat
     6721 cctatcttca ccaatgaaaa tttccacccc agagattata agcggaatca gcaacatcca
     6781 ttactatttc tatgatttag tcatttcaaa gtgccgagaa gtaaatcttg atcccttaca
     6841 agtatcaaca ttacttggac aaaccttacc taaaagagtt gatatgtctt acattgaatc
     6901 agttattgat caaaatttta gacaggagag tgcaaaatga aagctaaaaa attacgtgag
     6961 ttgctatatt caaatcaagt tgtgcgagta atgggggcgc ataatggttt aagcgctaaa
     7021 cttgctgaac aagccgggtt ccatgcaata tgggccagcg gattagaaat ttcggcatct
     7081 tatgctgttc ctgatgctaa tattttgact atgacagaaa atctgcaagc cgcagtcgtg
     7141 atgaatgaat ctacatccat cccaatcatt tgtgattgcg attctggtta tggaagtgtg
     7201 aacaatgtca tacgtatggt aaaagaatat gaaagaaacg gaattgctgg tatttgtata
     7261 gaagataagc aatttcctaa attaaatagt tttgtgaagg gctcgcaaaa attggcagat
     7321 attgatgagt tctcaaataa aatcagagcg gcaaaagatg ttcaaaaaaa tcctgatttt
     7381 gttgttattg ccagaataga agccttaatt gcgggtcaag gaatggatga agcattaaac
     7441 agagcctatg cttatgaggc agcaggagct gatgccattc tgatccattc gaaagaaaac
     7501 cagccaaacg aaattaagga attcgtaaaa cagtttactg gagcagtacc tattgtgatt
     7561 gtacctacga catacccgca tataaccgtg aaagaaatgg agttattggg catcaatatg
     7621 gtcatttatg ccaaccacgg tcttagaagt tctattaagg caatgcaaga gacattctcc
     7681 cagattctcc ttgatggaaa tacggtaggt gtagaagata acattgtgtc tatgaaaact
     7741 gttttcgaac ttcaaggtat gtatgacatg agaaaacaag aagatatgta taactccggc
     7801 acttctgtca tcagtactat caaataaaat aagataggag taatttagaa tgatttctga
     7861 ccaatataat attgtttcac tagcatattt acattcaaaa attaattttt ttattgaacc
     7921 gtcattatca ttagaccatg tttttgattt tttcagtaca cactttttta ttaacagggg
     7981 aagacatgaa gaagatcgtt tggctgatgt atacatgact cataataaag aagaatttca
     8041 aacgatagac tttaataacg gtgaggatat ttatatcaga aaaagtgcta ctgattattt
     8101 caccattcct tgtaaacgag taactgttga agggattgaa tatgttaaat gtacaaaaac
     8161 agatacgatc ctttcatttg ataaagaaaa caagaaaata atcatttcta cacagttaca
     8221 aaatgcagag caggatgaac tggttttaat tgagttcatt agagatttag tattgaaaaa
     8281 tgaagagaac catggtgtgg cggtggtgca tgcgaccagt gcgttaaaag atgacaaagc
     8341 aactctcatt ataggttcaa aagggaaagg caagtcaact ttattgctgg aattagtgag
     8401 taaacacggg tacaaactca taagcggtga taagactttt ctttggatgg aagatgggaa
     8461 attacgggcc tcagggtggc cggattatcc tcatttggga ctcggcacac tttctaaata
     8521 tccggaattc gtcgaacact tcgggctctc taataaaatt gaatcagtaa aagaaaatct
     8581 ttggtcaact gaacataaaa tggccattga tccggttttt tttaagaagg ttattccttt
     8641 tgcagaacca gggacatcat ttatggtcga atgtatgatt taccctgatc tatttccttc
     8701 aacagaatgc ggaatcaagc aggtaagtaa tcatggtgaa ttgattgtac cgcatataga
     8761 aaggattttt acgaaaggga acacgatgtg gaaccattat attcggcctg tacatcagaa
     8821 tgaactggag agaaaaataa atcagtgtat tgagcttgct ggaaagcttt ctgcttttga
     8881 agtgagcgga tcaggcattt tagagggttc agtgtttagc gagagatacg ctctgtaatc
     8941 tttgaaaatt tggaaataaa ggagaaaaat aaagtggttg aaatcacgat tcaatcaaaa
     9001 gggtttaatc acagttatga cattacagaa caagtaacag acgcattatc tcaattagag
     9061 ccatcttcag gtttagcaaa ggtctgtgta accggcagta cggtaggtct cactgttatg
     9121 cgttatgaac caggcaccat acacgacttg ctccaatgtt tagagtccat tgcttcaaaa
     9181 aataaacaat atgaacactt taacacgacc ggtgatccaa atggcttttc acatgtgtgg
     9241 tcttcaatga ttgggacaag tgtgatggtt ccatacaaaa atcataagct agcttgttcg
     9301 gacagccata gggtcgtatt atttgatttt gatttaaggg aagcagaacg aaaaatctat
     9361 gtagatcgtt aatgaatata ttaggggagt tgtttgagat gaggaagtta cggatggcta
     9421 ctccgggacc tacgcaagtt ccgcaggaaa ttttattggc gggagcacag gaaatgattc
     9481 atcaccgatc ccggcaaatg gaggatatta ttaatgaaat taatcgtgaa ctcccagatc
     9541 tttttttgac aaaacaagat atttatattt tgttatcttc aggtacgggg gctatggagg
     9601 ctgctgtatc aaactccttt aatagggggg ataaagtttt agtcgtctct aatggttact
     9661 ttggagagcg ttttactgat atctgtacag tattcggttt agaggtgata accgtaagct
     9721 ctccttgggg aacttctgtt aatattgaag aagttgaaag agcgtataag cagcatccgg
     9781 atataaaggg agtgctcact gtttacagtg aaacctcaac gggagcagtt aatgatgtaa
     9841 aggccattgg atcattgttc agagatacag aggtgctggt gattgttgat gctataagcg
     9901 gccttatttc tcatgagcta tcaatggatg attggggatt agacatagta ttagcagcct
     9961 ctcacaaagg atttatgatg ccgccgggct tagcgtttgt tgctatttcg ccgaaagctt
    10021 ggaaagtgat taatgaaatt cagccttcaa cttactattt ttcatttaaa agatttaaaa
    10081 aattttatcc tatggcacct tcaagtccgg gtgtatcttt actattagca ttaaaaaaat
    10141 cactagattt attgaaagca gaaggccttt cgaatgctac cagacgacat gcccgtatcg
    10201 ctagggcaac gcaaagagga ttggaagctt taggttttca cctttttgta caatctcctc
    10261 atgttagaag tcatacagtg acagcagcgt tagctccatc gggaattgat gcagctttat
    10321 tattaaaaaa actaaataaa gaatatggac ttacgataac agggggtcaa ggtgagttta
    10381 aagggaagat gatcaggatt ggacatattg gagctattga tgaaattgat ttgttcggaa
    10441 tattcggaat aatggaattg gtgttaacag aaatgggtta tcaggttcag tctgggatca
    10501 gcatgactgc tttgagtgat gcattaggag ggggagtcat acaccatgct taaattatat
    10561 cagagggaac catgtcctta ttgcaaaccg gtaagagaga aattaactga gctgaatgta
    10621 acgtatatca atgtgaatct tccgaaggat cgtgctttac gaacagaatt aatagaaaaa
    10681 acaggcgttc cttatatacc cgctttaatt gatgaagaag ataacgttat catttcaggt
    10741 aaactggaat acaaccagca tgtcttggat tatatagaaa aaaagtttgg ccaaaatcgt
    10801 taagggggat tcttcttctt gcgctatgcc ttcataacag atatacatgg gaaacgtgat
    10861 aatcttgaaa aggttttgga gcatatccaa accttttctt ctatacatga gatcatttgt
    10921 ttgggagatg tttttgaagt taaggttcct aaaaaggatt taaaagattt tcagtttcag
    10981 tgcatggaac aagttttaga tttggattgg gaattaatga atttgttaaa agatattcga
    11041 gtgataaaag gtaatcaaga ggagaggata ttaagtctga tcccagagaa agaatttccg
    11101 aaagactttt acaacttttt gattcacatg cctttagaaa ttcatttaac tccaatggtc
    11161 agattaacgc atggccatac ttttgattgg acgggagaag aagattattg gaggcccagc
    11221 aatgttcatt attggagcag cccgtggatc ttttatggtc ataaccacca aaatgtactg
    11281 tttgaagtta aggagataga gaataatcct tattatgaac ggaaaagcat tataaacggt
    11341 aagcctatca cattagactc tgcatctcgt tatttgatca atataggtga tatgaaatat
    11401 gatcatccta gttggatgct tttggataca ttggaaaatc agattactta caatatattg
    11461 tattgaaaag gagtttattc tgatgcttcg tattttactc attaattccg ataaaccaga
    11521 gcctattcag ttttttcaaa aagataagga aacaaatgat tctatcaata tatctgtcat
    11581 tacgagatcg tgctatgccc ctctttattc acattgggca gatcatgtat acatcgttga
    11641 tgatgtaaca gatttaacgg tgatgaagag tttgatgctg gagatattaa aagtggggcc
    11701 aattgatcat attgtttcaa caaccgaaaa gagtatatta acaggcgggt ttcttcgctc
    11761 ctattttggg atagccgggc ctggatttga aacagctctt tatatgacga ataaattggc
    11821 aatgaagact aaacttaaaa tggaagggat tcctgttgca gattttttgt gtgtaagcca
    11881 agtagaggat atccctgcag caggcgagaa actaggctgg cctattattg taaagccggc
    11941 tcttggttcg ggcgccttaa atacttttat catccattca ttagatcatt acgaagacct
    12001 gtattcaaca tcgggtggtt taggcgaact aaagaagaat aactcactta tgattgctga
    12061 aaaatgtata gaaatggaag agtttcattg tgatacttta tacgctgacg gagaaattct
    12121 ttttgtatca atatcaaaat atacagtgcc tttgctaaaa ggaatggcta aaatccaagg
    12181 gtcatttatt ttgagtcaaa atgatccggt ttatgctgaa atattagaac ttcagaagtc
    12241 tgttgctcaa gcgtttcgta tcacagacgg tcccgggcat cttgaaatat acagaaccca
    12301 ttcaggtgaa ctgatcgtcg gtgagattgc aatgcgtatt gggggcggag ggatcagccg
    12361 catgatagaa aaaaaattca atatatcttt atgggaaagt tctcttaaca tttccgtata
    12421 tagagatcca aatctcacgg tcaatccaat agagggaact gtcggatact ttagcttgcc
    12481 ttgccgaaac ggaacaataa aagaatttac gcccatcgag gaatgggaaa agcttgctgg
    12541 catactggag gttgaattgt tataccaaga aggtgatgtt gtagatgaaa agcaaagttc
    12601 aagttttgat ctggccaggc tttatttttg tttagaaaat gagaatgaag tacaacatct
    12661 attagctcta gtcaaacaaa catattatct tcacttaaca gaggatcata tgatgaacca
    12721 ataa
//
