LOCUS       BGC0000936              6720 bp    DNA     linear   BCT 14-DEC-2001
DEFINITION  Pseudomonas aeruginosa pyocyanine biosynthesis operon, complete
            sequence.
ACCESSION   BGC0000936
VERSION     BGC0000936
KEYWORDS    .
SOURCE      Pseudomonas aeruginosa PAO1
  ORGANISM  Pseudomonas aeruginosa PAO1
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
COMMENT     On Jun 23, 1999 this sequence version replaced AF005404.1.
            ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2024-10-17 14:27:05
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6720
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..6720
                     /db_xref="taxon:208964"
                     /mol_type="genomic DNA"
                     /organism="Pseudomonas aeruginosa PAO1"
                     /strain="PAO1"
     subregion       1..6720
                     /aStool="mibig"
                     /contig_edge="True"
                     /label="other"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..6720
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     regulatory      419..422
                     /gene="phzA"
                     /regulatory_class="ribosome_binding_site"
     gene            430..918
                     /gene="phzA"
     CDS             430..918
                     /codon_start=1
                     /gene="phzA"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phenazine: phzB"
                     /gene_kind="biosynthetic"
                     /note="similar to Pseudomonas fluorescens gene product
                     PhzA; similar to Pseudomonas aureofaciens gene product Phz"
                     /product="PhzA"
                     /protein_id="AAC64489.1"
                     /sec_met_domain="phzB (E-value: 8.5e-104, bitscore: 335.9,
                     seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNGQRYRETPLDIERLRRLNRATVERYMAMKGAERLQRHSLFVED
                     GCAGNWTTESGEPLVFRGHESLRRLAEWLERCFPDWEWHNVRIFEAEDPNHFWVECDGR
                     GKALVPGYPQGYCENHYIHSFELENGRIKRNREFMNPIQKLRALGIAVPQIKRDGIPT"
     gene            430..6685
                     /gene="pyocyanine biosynthesis operon"
     regulatory      936..939
                     /gene="phzB"
                     /regulatory_class="ribosome_binding_site"
     gene            948..1436
                     /gene="phzB"
     CDS             948..1436
                     /codon_start=1
                     /gene="phzB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phenazine: phzB"
                     /gene_kind="biosynthetic"
                     /note="similar to Pseudomonas fluorescens gene product
                     PhzB; similar to Pseudomonas aureofaciens gene product
                     PhzY"
                     /product="PhzB"
                     /protein_id="AAC64490.1"
                     /sec_met_domain="phzB (E-value: 2.9e-89, bitscore: 288.1,
                     seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPDTTNPIGFTDANELREKNRATVEKYMNTKGQDRLRRHELFVED
                     GCGGLWTTDTGSPIVIRGKDKLAEHAVWSLKCFPDWEWYNINIFGTDDPNHFWVECDGH
                     GKILFPGYPEGYYENHFLHSFELEDGKIKRNREFMNVFQQLRALSIPVPQIKREGIPT"
     regulatory      1446..1450
                     /gene="phzC"
                     /regulatory_class="ribosome_binding_site"
     gene            1460..2677
                     /gene="phzC"
     CDS             1460..2677
                     /codon_start=1
                     /gene="phzC"
                     /note="similar to plant phospho-2-keto-3-deoxyheptonate
                     aldolases (DAHP-synthases)"
                     /product="PhzC"
                     /protein_id="AAC64491.1"
                     /transl_table=11
                     /translation="MDDLLQRVRRCEALQQPEWGDPSRLRDVQAYLRGSPALIRAGDIL
                     ALRATLARVARGEALVVQCGDCAEDMDDHHAENVARKAAVLELLAGALRLAGRRPVIRV
                     GRIAGQYAKPRSKPHEQVGEQTLPVYRGDMVNGREAHAEQRRADPQRILKGYAAARNIM
                     RHLGWDAASGQEANASPVWTSHEMLLLDYELSMLREDEQRRVYLGSTHWPWIGERTRQV
                     DGAHVALLAEVLNPVACKVGPEIGRDQLLALCERLDPRREPGRLTLIARMGAQKVGERL
                     PPLVEAVRAAGHPVIWLSDPMHGNTIVAPCGNKTRLVRSIAEEVAAFRLAVSGSGGVAA
                     GLHLETTPDDVTECVADSSGLHQVSRHYTSLCDPRLNPWQALSAVMAWSGAEAIPSATF
                     PLETVA"
     regulatory      2662..2665
                     /gene="phzD"
                     /regulatory_class="ribosome_binding_site"
     gene            2674..3297
                     /gene="phzD"
     CDS             2674..3297
                     /codon_start=1
                     /gene="phzD"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1027:isochorismatase (Score: 287.5; E-value: 1.3e-87)"
                     /gene_kind="biosynthetic-additional"
                     /note="similar to isochorismatases"
                     /product="PhzD"
                     /protein_id="AAC64487.1"
                     /transl_table=11
                     /translation="MSGIPEITAYPLPTAQQLPANLARWSLEPRRAVLLVHDMQRYFLR
                     PLPESLRAGLVANAARLRRWCVEQGVQIAYTAQPGSMTEEQRGLLKDFWGPGMRASPAD
                     REVVEELAPGPDDWLLTKWRYSAFFHSDLLQRMRAAGRDQLVLCGVYAHVGVLISTVDA
                     YSNDIQPFLVADAIADFSEAHHRMALEYAASRCAMVVTTDEVLE"
     regulatory      3283..3286
                     /gene="phzE"
                     /regulatory_class="ribosome_binding_site"
     gene            3294..5177
                     /gene="phzE"
     CDS             3294..5177
                     /codon_start=1
                     /gene="phzE"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1018:isochorismate synthase (Score: 328; E-value:
                     2.1e-99)"
                     /gene_kind="biosynthetic-additional"
                     /note="similar to bacterial type-I glutamine
                     amidotransferases"
                     /product="PhzE"
                     /protein_id="AAC64488.1"
                     /transl_table=11
                     /translation="MNALPTSLLQRLLERPAPFALLYRPESNGPGLLDVIRGEALELHG
                     LADLPLDEPGPGLPRHDLLALIPYRQIAERGFEALDDGTPLLALKVLEQELLPLEQALA
                     LLPNQALELSEEGFDLDDEAYAEVVGRVIADEIGRGEGANFVIKRRFQARIDGYATASA
                     LSFFRQLLLREKGAYWTFIVHTGERTLVGASPERHISVRDGLAVMNPISGTYRYPPAGP
                     NLAEVMEFLDNRKEADELYMVVDEELKMMARICEDGGRVLGPYLKEMAHLAHTEYFIEG
                     QTSRDVREVLRETLFAPTVTGSPLESACRVIRRYEPQGRGYYSGVAALIGGDGQGGRTL
                     DSAILIRTAEIEGDGRLRIGVGSTIVRHSDPLGEAAESRAKASGLIAALKSQAPQRLGS
                     HPHVVAALASRNAPIADFWLRGASERQQLQADLSGREVLIVDAEDTFTSMIAKQLKSLG
                     LTVTVRGFQEPYSFDGYDLVIMGPGPGNPTEIGQPKIGHLHLAIRSLLSERRPFLAVCL
                     SHQVLSLCLGLDLQRRQEPNQGVQKQIDLFGAAERVGFYNTFAARALQDRIEIPEVGPI
                     EISRDRETGEVHALRGPRFASMQFHPESVLTREGPRIIADLLRHALVERRP"
     regulatory      5182..5185
                     /gene="phzF"
                     /regulatory_class="ribosome_binding_site"
     gene            5191..6027
                     /gene="phzF"
     CDS             5191..6027
                     /codon_start=1
                     /gene="phzF"
                     /note="similar to Pseudomonas fluorescens PhzF gene
                     product; similar to Pseudomonas aureofaciens PhzC gene
                     product"
                     /product="PhzF"
                     /protein_id="AAC64492.1"
                     /transl_table=11
                     /translation="MHRYVVIDAFASEPLQGNPVAVFFDCDDLSGERMQRMAREMNLSE
                     STFVLRPQQDGDARIRIFTPVNELPFAGHPLLGTAIALGAETDKDRLFLETRMGTVPFA
                     LERQDGKVVACSMQQPIPTWEHFSRPAELLAALGLKGSTFPIEVYRNGPRHVFVGLESV
                     AALSALHPDHRALCDFPDLAVNCFAGAGRHWRSRMFSPAYGVVEDAATGSAAGPLAIHL
                     ARHRQIPYGQQIEILQGVEIGRPSRMYARAEGAGERVSAVEVSGNGAAFAEGRAYL"
     regulatory      6039..6043
                     /gene="phzG"
                     /regulatory_class="ribosome_binding_site"
     gene            6053..6697
                     /gene="phzG"
     CDS             6053..6697
                     /codon_start=1
                     /gene="phzG"
                     /note="similar to bacterial pyridoxamine-5'-phosphate
                     oxidases"
                     /product="PhzG"
                     /protein_id="AAC64493.2"
                     /transl_table=11
                     /translation="MGVNANISESLTGTIEAPFPEFEAPPANPMEVLRNWLERARRYGV
                     REPRALALATVDGQGRPSTRIVVIAELGERGVVFATHADSQKGRELAQNPWASGVLYWR
                     ESSQQIILNGRAERLPDERADAQWLSRPYQTHPMSIASRQSETLADIHALRAEARRLAE
                     TDGPLPRPPGYCLFELCLESVEFWGNGTERLHERLRYDRDEGGWKHRYLQP"
ORIGIN
        1 ggaagcatca gcttagcaat cccgcatacc ctgtctggca cctaccagat cttgtagttg
       61 agccggtacg agcgttctgt gttttatgca atccacatca gcgaccaggg atgctggcta
      121 tttgaaacac ttcacggaat gacgctgaaa gtcttcgcga cctcgtctgt cgcaccttaa
      181 cgaaagcatt gcgaatccat taccgacagg tttccaaaag aaacccggga tgaaactcct
      241 attgcatttc gaaaattgga aacgacaggc gaacatatgt aacgcgaaat ttcaccctac
      301 gtataaacaa tgcgcccagc gaatatcgct cccttaccga gcgacgaact cctgcgcgcc
      361 agcgaataac cggtgccgcg agggaaaagt ttctccggca tacctggaga gccctctcgg
      421 aggcggcgca tgaacggtca gcggtacagg gaaacacccc tcgacatcga gcgtctgcgg
      481 cgcctgaatc gcgccacggt ggagcgctac atggcaatga agggggccga acggttacag
      541 cggcacagcc tgttcgtcga ggacggctgc gccggcaact ggaccacgga aagcggcgaa
      601 cccctggttt tccggggcca tgagagcctc aggcggctcg ccgagtggct cgagcgctgc
      661 ttccccgact gggagtggca caacgtgcgg atcttcgagg ccgaggatcc gaaccacttc
      721 tgggtcgagt gcgacgggcg cggcaaggcg ctggtcccgg ggtatccgca gggctattgc
      781 gagaaccact acatccattc cttcgaactc gagaacggcc ggataaaacg taatcgcgag
      841 ttcatgaacc cgatacagaa actgcgtgca ttgggaatag ccgttccgca aataaaacgt
      901 gacggtattc ccacttgaat gacatctacc gccaaggagc attgacgatg cctgatacga
      961 caaatccaat cggtttcacc gatgccaacg aacttcgcga aaagaatcgc gccaccgtcg
     1021 agaagtacat gaataccaaa ggccaggatc gcctgcgccg ccatgaactt ttcgtcgagg
     1081 acggctgtgg cggtttatgg accaccgata ccggctcgcc catcgtcatt cgtggcaaag
     1141 acaaacttgc ggaacatgcc gtctggtcat tgaagtgctt tcccgattgg gagtggtaca
     1201 acataaatat attcggaacc gacgacccca atcatttctg ggtcgaatgc gacggtcacg
     1261 gcaagattct tttccccgga tatcccgaag gatattacga aaaccacttc ctgcattcct
     1321 tcgaacttga agacggcaag atcaagcgca accgcgaatt catgaacgtc ttccagcaat
     1381 tgcgcgcctt gagcattccg gtcccgcaga tcaaacgcga aggcattccc acctgaggcc
     1441 atcctggaag gggtgaacta tggacgatct attgcaacgc gtacggcgct gcgaagcgct
     1501 gcagcaaccc gaatggggcg atccgtcgcg cctgcgcgac gtgcaggcgt acctgcgcgg
     1561 cagtccggcg ctgatccgcg ccggcgacat cctggccctg cgcgcgaccc tggcgcgggt
     1621 cgcccgcggc gaggcgctgg tggtacagtg cggcgactgc gccgaggaca tggacgacca
     1681 ccatgccgag aacgtggcgc gcaaggccgc cgtgctggaa ctgctggccg gcgccctgcg
     1741 cctggccggc cggcggccgg tgatccgcgt cgggcgcatc gccgggcagt acgccaagcc
     1801 gcgttccaag ccgcacgagc aggtcggcga gcagaccctg ccggtctatc gcggcgacat
     1861 ggtcaacggc cgcgaggccc atgccgaaca gcgccgggcc gatccgcagc ggatcctcaa
     1921 gggctatgcg gcggcgcgca acatcatgcg ccacctgggc tgggacgccg cgtccgggca
     1981 ggaggcgaat gcctcgccgg tctggaccag ccacgagatg ctgctgctcg actacgagct
     2041 gtcgatgctg cgcgaggacg agcagcgccg ggtctatctc ggttcgaccc actggccgtg
     2101 gatcggcgag cgcacccgcc aggtcgacgg cgcccatgtg gcgctgctgg ccgaggtgct
     2161 caacccggtg gcctgcaagg tcggtccgga gatcggccgc gaccagttgc tggcgctctg
     2221 cgagcgcctc gatccgcgcc gcgagccggg acgcctgacg ctgatcgcgc ggatgggcgc
     2281 gcagaaggtc ggcgagcgcc tgccgccgct ggtggaggcg gtgcgcgcgg ccgggcaccc
     2341 ggtgatctgg ctgagcgacc cgatgcacgg caacaccatc gtcgcgccct gcggcaacaa
     2401 gacccgcctg gtgcgcagca tcgccgagga ggtggcggcg ttccgcctgg cggtgtccgg
     2461 ctccggcggc gtggccgccg ggctgcacct ggaaaccacc ccggacgacg tcaccgagtg
     2521 cgtcgccgat tccagcggcc tgcaccaggt cagccggcac tacaccagcc tctgcgatcc
     2581 gcggctgaac ccctggcagg cgctcagcgc ggtgatggcc tggtccggcg cagaagcgat
     2641 cccgagcgca accttccccc tggagaccgt ggcatgagcg gcattcccga aatcaccgcc
     2701 tatcccttgc cgaccgccca gcaactgccg gccaacctgg cgcgctggag cctggagccg
     2761 cggcgcgcgg tgctgctggt gcacgacatg cagcgctact tcctacgccc gctgcccgag
     2821 agcctgcgcg ccggactggt ggccaacgcc gcgcggctgc gccgctggtg cgtcgagcag
     2881 ggcgtgcaga tcgcctacac cgcccagccg ggcagcatga ccgaggagca gcgcggtctg
     2941 ctcaaggact tctgggggcc gggcatgcgc gccagtccgg ccgaccgcga ggtggtcgag
     3001 gagctggcgc cggggccgga cgactggctg ctgaccaagt ggcgctacag cgccttcttc
     3061 cactccgacc tgctccagcg gatgcgcgcc gccggccgcg accagttggt gctgtgcggc
     3121 gtatacgccc acgtcggggt gctgatctcc acggtcgacg cctactccaa cgacatccag
     3181 ccgttcctgg tggccgacgc catcgccgac ttcagcgagg cgcaccaccg catggccctg
     3241 gaatacgccg ccagccgctg cgcgatggtg gtcaccaccg acgaggtgct ggaatgaacg
     3301 ccctgcccac atcgctgttg cagcgcctgc tcgaacggcc ggcgccgttc gccttgctct
     3361 accgcccgga aagcaatggc ccgggcctgc tcgacgtgat ccgcggcgag gcgctcgaac
     3421 tgcacggcct ggccgacctg ccgctggacg agcccggccc gggcctgccg cgccatgacc
     3481 tgctggcgct gattccctac cggcagatcg ccgagcgcgg cttcgaggcg ctcgacgacg
     3541 gcacgccgct gctggcgctg aaggtcctgg agcaggaact gctgccgctg gagcaggccc
     3601 tggcgctgct gccgaaccag gcgctggaac tgagcgagga aggtttcgac ctcgacgacg
     3661 aggcctacgc cgaagtggtc gggcgggtca tcgccgacga gatcggtcgc ggcgaaggcg
     3721 ccaacttcgt gatcaagcgg cgcttccagg cgcgcatcga cggctacgcc acggccagcg
     3781 cgctgagctt cttccgccag ttgctgctgc gcgagaaggg cgcctactgg accttcatcg
     3841 tccacaccgg cgaacgcacc ctggtcggcg cctcgcccga gcgacacatc agcgtgcgcg
     3901 acggcctggc ggtgatgaac ccgatcagcg gcacctaccg ctacccgccg gccgggccga
     3961 acctggccga ggtcatggaa ttcctcgaca accgcaagga agccgacgag ctgtacatgg
     4021 tggtggacga ggagctgaag atgatggcgc ggatctgcga ggacggcggc cgggtgctcg
     4081 gtccctacct caaggagatg gcgcacctgg cgcacaccga gtacttcatc gagggccaga
     4141 ccagccgcga cgtgcgcgag gtgctgcgcg agacgctgtt cgccccgacc gtcaccggca
     4201 gcccgctgga aagcgcctgc cgggtgatcc gccgctacga gccgcagggt cgcggctact
     4261 acagcggggt ggcggcgctg atcggcggtg atggccaggg cgggcgcacg ctggactcgg
     4321 cgatcctgat ccgcaccgcc gagatcgagg gcgacggccg cctgcgcatc ggcgtcggct
     4381 cgaccatcgt gcgtcactcc gatcccctgg gcgaggcggc ggaaagccgg gccaaggctt
     4441 ccgggctgat cgccgcgctg aagagccagg cgccgcagcg cctgggctcg cacccgcacg
     4501 tggtggcggc gctggccagc cgcaacgcgc cgatcgccga cttctggctg cgcggcgcca
     4561 gcgagcgcca gcagttgcag gccgacctgt ccggacgcga ggtgctgatc gtcgatgccg
     4621 aggacacctt cacctcgatg atcgccaagc agctcaagtc gctgggcctg acggtgaccg
     4681 tgcgcggctt ccaggagccg tactcgttcg acggctacga cctggtgatc atgggccccg
     4741 gcccaggcaa ccccaccgag atcggccagc cgaagatcgg ccacctgcac ctggccatcc
     4801 gttcgctgct cagcgagcgc cggccgttcc tcgccgtgtg cctgagccac caggtgctga
     4861 gcctgtgcct gggcctggac ctgcaacgcc ggcaagagcc caaccagggc gtgcagaaac
     4921 agatcgacct gttcggcgcc gccgagcgcg tcggcttcta caacaccttc gctgcgcgcg
     4981 ccctgcagga ccgcatcgag atccccgagg tcggcccgat cgagatcagc cgcgaccgcg
     5041 agaccggcga ggtccatgcc ctgcgcgggc cgcgcttcgc ctccatgcaa ttccaccccg
     5101 aatcggtgct gacccgcgag ggtccgcgca tcatcgccga cctgctgcgt cacgcgctgg
     5161 tcgagcggcg gccctgagcc aggagagccc atgcacagat atgtcgtgat agacgccttc
     5221 gccagcgaac cgctgcaagg caacccggtg gcggtgttct tcgactgcga cgacctcagc
     5281 ggcgagcgca tgcagcgcat ggcgcgggag atgaacctgt cggagagcac cttcgtgctc
     5341 cgcccgcaac aggacggcga cgcgcgcatc cgcatcttca ccccggtcaa cgaactgccg
     5401 ttcgccggcc acccgctgct gggcaccgcc atcgccctgg gcgccgagac cgacaaggac
     5461 aggctgttcc tggagaccag gatgggcacc gtgcccttcg ccctggaacg ccaggacggc
     5521 aaggtggtgg cctgcagcat gcagcagccg atccccacct gggagcactt cagccgaccg
     5581 gccgagctgc tcgccgccct cggcctgaag ggctcgacct ttcccatcga ggtctaccgc
     5641 aacggcccgc gccacgtctt cgtcggcctg gagagcgtcg ccgcgctgtc cgcgctgcac
     5701 cccgaccacc gcgcgctgtg cgacttcccg gacctggcgg tgaactgctt cgccggcgcc
     5761 gggcggcact ggcgcagccg catgttctcg ccggcctacg gcgtggtcga ggacgccgcc
     5821 accggttccg ccgccgggcc gctggcgatc cacctggcgc gccaccggca gattccctac
     5881 gggcagcaga tcgagatcct ccagggcgtc gagatcggcc ggccgtcgcg catgtacgcc
     5941 cgcgccgagg gcgccggcga gcgggtctcg gcggtggaag tgtcgggcaa cggcgcggcc
     6001 ttcgccgaag gccgcgccta cctgtgaacg ggcagaacgg aggaacgccg tgatgggcgt
     6061 caacgcgaac atttccgagt ccctcaccgg gaccatcgaa gcccctttcc cggagttcga
     6121 ggcgccgccg gcgaacccca tggaggtgct gcgcaactgg ctggaacgcg cccgccgcta
     6181 cggcgtgcgc gagccgcggg cactggccct ggccacggtc gacgggcagg gccggccgtc
     6241 gacgcggatc gtggtgatcg ccgagctggg cgagcgcggg gtggtgttcg ccacccacgc
     6301 cgatagccag aagggccgcg aactggcgca gaacccctgg gcctcggggg tgctgtactg
     6361 gcgcgagagc agccagcaga tcatcctcaa cggccgcgcc gaacgcctgc ccgacgagcg
     6421 cgccgacgcc cagtggctga gccgcccgta ccagacccac ccgatgtcga tcgcctcgcg
     6481 gcagagcgaa accctcgccg acatccacgc cctgcgcgcc gaagcccggc gcctggcgga
     6541 aaccgacggc ccgctgccgc gcccgccggg ctactgcctg ttcgaactgt gcctggagtc
     6601 ggtggagttc tggggcaacg gcaccgagcg gctgcacgag cgcctgcgct acgaccgcga
     6661 cgaaggaggc tggaagcacc gctacctgca accgtgacga caccgctgcg ccggcgtttc
//
