LOCUS       BGC0000943             14447 bp    DNA     linear   BCT 17-JUN-2018
DEFINITION  Staphylococcus aureus strain NCTC8325 genome assembly, chromosome:
            1.
ACCESSION   BGC0000943
VERSION     BGC0000943
KEYWORDS    .
SOURCE      Staphylococcus aureus subsp. aureus NCTC 8325
  ORGANISM  Staphylococcus aureus subsp. aureus NCTC 8325
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 14:28:10
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..14447
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[5237:11726]"
                     /cutoff="20000"
                     /detection_rule="IucA_IucC"
                     /neighbourhood="14000"
                     /product="NI-siderophore"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5238..11726
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="IucA_IucC"
                     /neighbourhood="14000"
                     /product="NI-siderophore"
                     /protocluster_number="1"
     cand_cluster    1..14447
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="IucA_IucC"
                     /kind="single"
                     /product="NI-siderophore"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..14447
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000943"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..14447
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NI-siderophore"
                     /region_number="1"
                     /rules="IucA_IucC"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            complement(1..999)
                     /gene="sirC_1"
                     /locus_tag="NCTC8325_00062"
     CDS             complement(1..999)
                     /codon_start=1
                     /gene="sirC_1"
                     /gene_functions="transport (smcogs) SMCOG1011:transport
                     system permease protein (Score: 254.8; E-value: 1.7e-77)"
                     /gene_kind="transport"
                     /inference="ab initio prediction:Prodigal:2.60"
                     /inference="similar to AA sequence:RefSeq:YP_006708863.1"
                     /locus_tag="NCTC8325_00062"
                     /product="siderophore transport system permease"
                     /protein_id="SQF72442.1"
                     /transl_table=11
                     /translation="MTEKINKKDNYHLIFALIFLAIVSVVSMMIGSSFIPLQRVLMYFI
                     NPNDSMDQFTLEVLRLPRITLAILAGAALGMSGLMLQNVLKNPIASPDIIGITGGASLS
                     AVVFIAFFSHLTIHLLPLFAVLGGAVAMMILLVFQTKGQIRPTTLIIIGISMQTLFIAL
                     VQGLLITTKQLSAAKAYTWLVGSLYGATFKDTIILGMVILAVVPLLFLVIPKMKISILD
                     DPVAIGLGLHVQRMKLIQLITSTILVSMAISLVGNIGFVGLIAPHIAKTIVRGSYAKKL
                     LMSAMIGAISIVIADLIGRTLFLPKEVPAGVFIAAFGAPFFIYLLLTVKKL"
     gene            complement(996..1991)
                     /gene="sirB"
                     /locus_tag="NCTC8325_00063"
     CDS             complement(996..1991)
                     /codon_start=1
                     /gene="sirB"
                     /gene_functions="transport (smcogs) SMCOG1011:transport
                     system permease protein (Score: 288.7; E-value: 8.4e-88)"
                     /gene_kind="transport"
                     /inference="ab initio prediction:Prodigal:2.60"
                     /inference="similar to AA sequence:RefSeq:YP_005740864.1"
                     /locus_tag="NCTC8325_00063"
                     /product="Siderophore staphylobactin ABC transporter,
                     permease protein SirB"
                     /protein_id="SQF72443.1"
                     /transl_table=11
                     /translation="MLLKPKYQIVIAGLCLAIVAILSLMIGNTLVSPGTVIQALFNFDS
                     ENDLHDVVTGARASRTIIALLTGAALAVSGLLMQALTRNPIASPGLFGVNAGAVFFVIF
                     SITFIQIQSFKMIVVIAFLGAIVVTVLVVALGMFRQTLFSPHRVILAGAAIAMLFTAFT
                     QGILIMNETDLQGLLFWLSGSVSLRNIWDIPWIIPLVLILILIAFSMAAHINILMTSDD
                     IATGLGQNIKLIKWMIIMLISMLAGISVAVAGSIVFVGLIVPNISKRLLPPNYKYLIPF
                     TALAGAILMIISDIVARIIIKPLELPIGVVTAVIGAIVLIYIMKKGRQRL"
     gene            complement(2007..2999)
                     /gene="sirA"
                     /locus_tag="NCTC8325_00064"
     CDS             complement(2007..2999)
                     /codon_start=1
                     /gene="sirA"
                     /gene_functions="transport (smcogs) SMCOG1033:iron compound
                     ABC transporter, periplasmic (Score: 253.2; E-value:
                     4.7e-77)"
                     /gene_kind="transport"
                     /inference="ab initio prediction:Prodigal:2.60"
                     /inference="similar to AA sequence:RefSeq:YP_005740865.1"
                     /locus_tag="NCTC8325_00064"
                     /product="Siderophore staphylobactin ABC transporter,
                     substrate-binding protein SirA"
                     /protein_id="SQF72444.1"
                     /transl_table=11
                     /translation="MNKVIKMLVVTLAFLLVLAGCSGNSNKQSSDNKDKETTSIKHAMG
                     TTEIKGKPKRVVTLYQGATDVAVSLGVKPVGAVESWTQKPKFEYIKNDLKDTKIVGQEP
                     APNLEEISKLKPDLIVASKVRNEKVYDQLSKIAPTVSTDTVFKFKDTTKLMGKALGKEK
                     EAEDLLKKYDDKVAAFQKDAKAKYKDAWPLKASVVNFRADHTRIYAGGYAGEILNDLGF
                     KRNKDLQKQVDNGKDIIQLTSKESIPLMNADHIFVVKSDPNAKDAALVKKTESEWTSSK
                     EWKNLDAVKNNQVSDDLDEITWNLAGGYKSSLKLIDDLYEKLNIEKQSK"
     gene            3230..4210
                     /gene="sbnA"
                     /locus_tag="NCTC8325_00065"
     CDS             3230..4210
                     /EC_number="2.5.1.47"
                     /codon_start=1
                     /gene="sbnA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PALP"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1081:cysteine synthase (Score: 374.4; E-value:
                     6.1e-114)"
                     /gene_kind="biosynthetic-additional"
                     /inference="ab initio prediction:Prodigal:2.60"
                     /inference="similar to AA sequence:RefSeq:YP_005740866.1"
                     /locus_tag="NCTC8325_00065"
                     /product="Siderophore staphylobactin biosynthesis protein
                     SbnA"
                     /protein_id="SQF72445.1"
                     /sec_met_domain="PALP (E-value: 1.5e-61, bitscore: 198.0,
                     seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIEKSQACHDSLLDSVGQTPMVQLHQLFPKHEVFAKLEYMNPGGS
                     MKDRPAKYIIEHGIKHGLITENTHLIESTSGNLGIALAMIAKIKGLKLTCVVDPKISPT
                     NLKIIKSYGANVEMVEEPDAHGGYLMTRIAKVQELLATIDDAYWINQYANELNWQSHYH
                     GAGTEIVETIKQPIDYFVAPVSTTGSIMGMSRKIKEVHPNAQIVAVDAKGSVIFGDKPI
                     NRELPGIGASRVPEILNRSEINQVIHVDDYQSALGCRKLIDYEGIFAGGSTGSIIAAIE
                     QLITSIEEGATIVTILPDRGDRYLDLVYSDTWLEKMKSRQGVKSE"
     gene            4207..5217
                     /gene="sbnB"
                     /locus_tag="NCTC8325_00066"
     CDS             4207..5217
                     /EC_number="4.3.1.12"
                     /codon_start=1
                     /gene="sbnB"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1158:ornithine cyclodeaminase (Score: 323.6; E-value:
                     1.7e-98)"
                     /gene_kind="biosynthetic-additional"
                     /inference="ab initio prediction:Prodigal:2.60"
                     /inference="similar to AA sequence:RefSeq:YP_005740867.1"
                     /locus_tag="NCTC8325_00066"
                     /product="Ornithine cyclodeaminase / Siderophore
                     staphylobactin biosynthesis protein SbnB"
                     /protein_id="SQF72446.1"
                     /transl_table=11
                     /translation="MNREMLYLNRSDIEQAGGNHSQVYVDALTEALTAHAHNDFVQPLK
                     PYLRQDPENGHIADRIIAMPSHIGGEHAISGIKWIGSKHDNPSKRNMERASGVIILNDP
                     ETNYPIAVMEASLISSMRTAAVSVIAAKHLAKKGFKDLTIIGCGLIGDKQLQSMLEQFD
                     HIERVFVYDQFSEACARFVDRWQQQRPEINFIATENAKEAVSNGEVVITCTVTDQPYIE
                     YDWLQKGAFISNISIMDVHKEVFIKADKVVVDDWSQCNREKKTINQLVLEGKFSKEALH
                     AELGQLVTGDIPGREDDDEIILLNPMGMAIEDISSAYFIYQQAQQQNIGTTLNLY"
     gene            5238..6992
                     /gene="sbnC"
                     /locus_tag="NCTC8325_00067"
     CDS             5238..6992
                     /EC_number="6.3.2.-"
                     /codon_start=1
                     /gene="sbnC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NI-siderophore: IucA_IucC"
                     /gene_kind="biosynthetic"
                     /inference="ab initio prediction:Prodigal:2.60"
                     /inference="similar to AA sequence:RefSeq:YP_005740868.1"
                     /locus_tag="NCTC8325_00067"
                     /product="Siderophore staphylobactin biosynthesis protein
                     SbnC"
                     /protein_id="SQF72447.1"
                     /sec_met_domain="IucA_IucC (E-value: 4.8e-60, bitscore:
                     192.7, seeds: 168, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQNHTAVNTAQAIILRDLVDALLFEDIAGIVSNSEITKENGQTLL
                     IYERETQQIKIPVYFSALNMFRYESSQPITIEGRVSKQPLTAAEFWQTIANMNCDLSHE
                     WEVARVEEGLTTAATQLAKQLSELDLASHPFVMSEQFASLKDRPFHPLAKEKRGLREAD
                     YQVYQAELNQSFPLMVAAVKKTHMIHGDTANIDELENLTVPIKEQATDMLNDQGLSIDD
                     YVLFPVHPWQYQHILPNVFAKEISEKLVVLLPLKFGDYLSSSSMRSLIDIGAPYNHVKV
                     PFAMQSLGALRLTPTRYMKNGEQAEQLLRQLIEKDEALAKYVMVCDETAWWSYMGQDND
                     IFKDQLGHLTVQLRKYPEVLAKNDTQQLVSMAALAANDRTLYQMICGKDNISKNDVMTL
                     FEDIAQVFLKVTLSFMQYGALPELHGQNILLSFEDGRVQKCVLRDHDTVRIYKPWLTAH
                     QLSLPKYVVREDTPNTLINEDLETFFAYFQTLAVSVNLYAIIDAIQDLFGVSEHELMSL
                     LKQILKNEVATISWVTTDQLAVRHILFDKQTWPFKQILLPLLYQRDSGGGSMPSGLTTV
                     PNPMVTYD"
     gene            7069..8241
                     /gene="sbnD"
                     /locus_tag="NCTC8325_00068"
     CDS             7069..8241
                     /codon_start=1
                     /gene="sbnD"
                     /gene_functions="transport (smcogs) SMCOG1020:major
                     facilitator transporter (Score: 87.5; E-value: 1.5e-26)"
                     /gene_kind="transport"
                     /inference="ab initio prediction:Prodigal:2.60"
                     /inference="similar to AA sequence:RefSeq:YP_005740869.1"
                     /locus_tag="NCTC8325_00068"
                     /product="Siderophore staphylobactin biosynthesis protein
                     SbnD"
                     /protein_id="SQF72448.1"
                     /transl_table=11
                     /translation="MPLLPIYMASLQNLSVVEIQLWSGIAIAAPAVTTMIASPIWGKLG
                     DKISRKWMVLRALLGLAVCLFLMALCTTPLQFVLVRLLQGLFGGVVDASSAFASAEAPA
                     EDRGKVLGRLQSSVSAGSLVGPLIGGVTASILGFSALLMSIAVITFIVCIFGALKLIET
                     THMPKSQTPNINKGIRRSFQCLLCTQQTCRFIIVGVLANFAMYGMLTALSPLASSVNHT
                     AIDDRSVIGFLQSAFWTASILSAPLWGRFNDKSYVKSVYIFATIACGCSAILQGLATNI
                     EFLMAARILQGLTYSALIQSVMFVVVNACHQQLKGTFVGTTNSMLVVGQIIGSLSGAAI
                     TSYTTPATTFIVMGVVFAVSSLFLICSTITNQINDHTLMKLWELKQKSAK"
     gene            8231..9967
                     /gene="sbnE"
                     /locus_tag="NCTC8325_00069"
     CDS             8231..9967
                     /EC_number="6.3.2.-"
                     /codon_start=1
                     /gene="sbnE"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NI-siderophore: IucA_IucC"
                     /gene_kind="biosynthetic"
                     /inference="ab initio prediction:Prodigal:2.60"
                     /inference="similar to AA sequence:RefSeq:YP_005740870.1"
                     /locus_tag="NCTC8325_00069"
                     /product="Siderophore staphylobactin biosynthesis protein
                     SbnE"
                     /protein_id="SQF72449.1"
                     /sec_met_domain="IucA_IucC (E-value: 2.3e-60, bitscore:
                     193.8, seeds: 168, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQNKELIQHAAYAAIERILNEYFREENLYQVPPQNHQWSIQLSEL
                     ETLTGEFRYWSAMGHHMYHPEVWLIDGKSKKITTYKEAIARILQHMAQSADNQTAVQQH
                     MAQIMSDIDNSIHRTARYLQSNTIDYVEDRYIVSEQSLYLGHPFHPTPKSASGFSEADL
                     EKYAPECHTSFQLHYLAVHQDVLLTRYVEGKEDQVEKVLYQLADIDISEIPKDFILLPT
                     HPYQINVLRQHPQYMQYSEQGLIKDLGVSGDSVYPTSSVRTVFSKALNIYLKLPIHVKI
                     TNFIRTNDLEQIERTIDAAQVIASVKDEVETPHFKLMFEEGYRALLPNPLGQTVEPEMD
                     LLTNSAMIVREGIPNYHADKDIHVLASLFETMPDSPMSKLSQVIEQSGLAPEAWLECYL
                     NRTLLPILKLFSNTGISLEAHVQNTLIELKDGIPDVCFVRDLEGICLSRTIATEKQLVP
                     NVVAASSPVVYAHDEAWHRLKYYVVVNHLGHLVSTIGKATRNEVVLWQLVAHRLMTWKK
                     EYANNAVFVDCVEDLYQTPTIAAKANLMSKLNDCGANPIYTHIPNPICHNKEVSYCESN
                     NS"
     gene            9948..11726
                     /gene="iucC_1"
                     /locus_tag="NCTC8325_00070"
     CDS             9948..11726
                     /EC_number="6.-.-.-"
                     /EC_number="6.3.2.-"
                     /codon_start=1
                     /gene="iucC_1"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NI-siderophore: IucA_IucC"
                     /gene_kind="biosynthetic"
                     /inference="ab initio prediction:Prodigal:2.60"
                     /inference="similar to AA sequence:RefSeq:YP_006194281.1"
                     /locus_tag="NCTC8325_00070"
                     /product="Siderophore synthase"
                     /protein_id="SQF72450.1"
                     /sec_met_domain="IucA_IucC (E-value: 1.4e-69, bitscore:
                     224.0, seeds: 168, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNQTILNRVKTRVMHQLVSSLIYENIVVYKASYQDGVGHFTIEGH
                     DSEYRFTAEKTHSFDRIRITSPIERVVGDEADTTTDYTQLLREVVFTFPKNDEKLEQFI
                     VELLQTELKDTQSMQYRESNPPATPETFNDYEFYAMEGHQYHPSYKSRLGFTLSDNLKF
                     GPDFVPNVKLQWLAIDKDKVETTVSRNVVVNEMLRQQVGDKTYEHFVQQIEASGKHVND
                     VEMIPVHPWQFEHVIQVDLAEERLNGTVLWLGESDELYHPQQSIRTMSPIDTTKYYLKV
                     PISITNTSTKRVLAPHTIENAAQITDWLKQIQQQDMYLKDELKTVFLGEVLGQSYLNTQ
                     LSPYKQTQVYGALGVIWRENIYHMLIDEEDAIPFNALYASDKDGVPFIENWIKQYGSEA
                     WTKQFLAVAIRPMIHMLYYHGIAFESHAQNMMLIHENGWPTRIALKDFHDGVRFKREHL
                     SEAASHLTLKPMPEAHKKVNSNSFIETDDERLVRDFLHDAFFFINIAEIILFIEKQYGI
                     DEELQWQWVKGIIEAYQEAFPELNNYQHFDLFEPTIQVEKLTTRRLLSDSELRIHHVTN
                     PLGVGGINDATTISET"
     gene            11698..12477
                     /gene="sbnG"
                     /locus_tag="NCTC8325_00071"
     CDS             11698..12477
                     /EC_number="4.1.2.20"
                     /codon_start=1
                     /gene="sbnG"
                     /inference="ab initio prediction:Prodigal:2.60"
                     /inference="similar to AA sequence:RefSeq:YP_005740872.1"
                     /locus_tag="NCTC8325_00071"
                     /product="Siderophore staphylobactin biosynthesis protein
                     SbnG"
                     /protein_id="SQF72451.1"
                     /transl_table=11
                     /translation="MMQQLSLKHRLNNGDSVYGIFNSIPDPLMIEVIAASGYDFVVIDT
                     EHVAINDETLAHLIRAAEAAHIIPIVRVTAVIDRDIIKVLDMGARGIIVPHVKDRETVE
                     HIVKLSRYYPQGLRSLNGGRMARFGRTPLLDAMEMANEHIMVIAMIEDVEGVMAIDDIA
                     QVEGLDMIVEGAADLSQSLGIPWQTRDDQVTSHVQHIFEVVNAHGKHFCALPREDEDIA
                     KWQAQGVQTFILGDDRGKIYRHLSASLATSKQKGDEG"
     gene            12477..13679
                     /gene="sbnH"
                     /locus_tag="NCTC8325_00072"
     CDS             12477..13679
                     /EC_number="4.1.1.20"
                     /codon_start=1
                     /gene="sbnH"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Orn_Arg_deC_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Orn_DAP_Arg_deC"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1264:diaminopimelate decarboxylase (Score: 308.8;
                     E-value: 6.4e-94)"
                     /gene_kind="biosynthetic-additional"
                     /inference="ab initio prediction:Prodigal:2.60"
                     /inference="similar to AA sequence:RefSeq:YP_005740873.1"
                     /locus_tag="NCTC8325_00072"
                     /product="Siderophore staphylobactin biosynthesis protein
                     SbnH"
                     /protein_id="SQF72452.1"
                     /sec_met_domain="Orn_Arg_deC_N (E-value: 1.5e-38, bitscore:
                     122.2, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="Orn_DAP_Arg_deC (E-value: 3.7e-17,
                     bitscore: 52.2, seeds: 500, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRIVQPVIEQLKAQSHPVCHYIYDLVGLEHHLQHITSSLPSNCQM
                     YYAMKANSERKILDTISQYVEGFEVASQGEIAKGLAFKPANHIIFGGPGKTDEELRYAV
                     SEGVQRIHVESMHELQRLNAILEDEDKTQHILLRVNLAGPFPNATLHMAGRPTQFGISE
                     DEVDDVIEAALAMPKIHLDGFHFHSISNNLDSNLHVDVVKLYFKKAKAWSEKHRFPLKH
                     INLGGGIGVNYADLTNQFEWDNFVERFKTLIVEQEMEDVTLNFECGRFIVAHIGYYVTE
                     VLDIKKVHGAWYAILRGGTQQFRLPVSWQHNHPFDIYRYKDNPYSFEKVSISRQDTTLV
                     GQLCTPKDVFAREVQIDAISTGDVIVFKYAGAYGWSISHHDFLSHPHPEFIYLTQTKED
                     E"
     gene            13683..14447
                     /gene="sbnI"
                     /locus_tag="NCTC8325_00073"
     CDS             13683..14447
                     /codon_start=1
                     /gene="sbnI"
                     /inference="ab initio prediction:Prodigal:2.60"
                     /inference="similar to AA sequence:RefSeq:YP_005740874.1"
                     /locus_tag="NCTC8325_00073"
                     /product="Siderophore staphylobactin biosynthesis protein
                     SbnI"
                     /protein_id="SQF72453.1"
                     /transl_table=11
                     /translation="MNHIHEHLKLVPVDKIDLHETFEPLRLEKTKSSIEADDFIRHPIL
                     VTAMQHGRYMVIDGVHRYTSLKALGCKKVPVQEIHETQYSISTWQHKVPFGVWWETLQQ
                     EHRLPWTTETRQEAPFITMCHGDTEQYLYTKDLGEAHFQVWEKVVASYSGCCSVERIAQ
                     GTYPCLSQQDVLMKYQPLSYKEIEAVVHKGETVPAGVTRFNISGRCLNLQVPLALLKQD
                     DDVEQLRNWKQFLADKFANMRCYTEKVYLVEQ"
ORIGIN
        1 ttataacttt ttcacggtta ataataagta tatgaagaat ggggcaccaa aagcagcaat
       61 aaatacacct gctggcactt ctttaggcaa gaataaggta cgcccaatta agtctgcaat
      121 aacaattgat atggcaccaa tcattgctga cattagtaac tttttagcat aacttccgcg
      181 aacgattgtt ttcgcgatat gtggtgcgat taaaccgaca aacccaatgt tacctactaa
      241 actgattgcc atagatacga gtatagtaga agtgattaat tggattagtt tcatacgttg
      301 tacatgtaag cctaagccaa tcgctacagg gtcatcaagt atagatattt tcatttttgg
      361 tataacaaga aataacaacg gcacaacagc taaaataacc atacccaaaa tgattgtatc
      421 tttaaacgta gcaccgtaaa gacttccgac tagccatgta taagctttgg cagcagataa
      481 ttgcttcgtt gtaatgagta atccttggac aagcgcaata aacaacgttt gcatcgaaat
      541 accgatgatt atgagtgttg tcgggcgtat ttgtcctttc gtttgaaaca ctaatagtat
      601 catcattgca actgcgccac ctaatactgc aaatagtgga agtaaatgta ttgttaaatg
      661 gctgaaaaat gcaataaaga caacagcact taagctagca ccacctgtga taccgataat
      721 atcaggtgag gcaattggat tttttaatac attttgcaac attaaaccac tcattcctag
      781 tgcggcacct gctaaaatcg caagtgtaat gcgaggtaag cgtaatactt ctaaagtgaa
      841 ttgatccata ctgtcatttg gatttataaa gtacatcagt acgcgttgta atggtataaa
      901 gcttgaacca atcatcatac ttaccactga aacgatggct aaaaagatta acgcgaagat
      961 gagatggtaa ttgtcttttt tattaatctt ttcggtcata agcgttgacg tcctttcttc
     1021 ataatataga ttaagacaat agcgccaatg acagcggtaa cgacaccgat aggcaactct
     1081 agtggcttaa ttattatacg agcaacaatg tctgaaatga tcattaggat tgctccagct
     1141 aatgcagtaa aaggaattaa atacttatag tttggtggta ataatcgttt gctaatattc
     1201 ggtacgataa gacccacaaa gacgattgat ccagctacgg ctaccgaaat accggctaac
     1261 atactgatga gcataataat catccatttg attaatttta tgttttgacc gaggccggtt
     1321 gcaatgtcgt cacttgtcat caagatgttg atgtgtgcag ccatgctaaa tgcaattaaa
     1381 ataagtatca atacaagcgg aataatccat gggatatccc aaatattacg taatgaaacg
     1441 gagccactta accaaaataa taggccttgt aagtctgttt cgttcataat aagtatgcct
     1501 tgagtaaagg ctgtaaatag catcgcaatc gcagcacctg ccaaaatgac acggtgaggt
     1561 gagaatagtg tttgtctaaa catacctagt gcaacaacta atacagtaac aacaatagcc
     1621 cccaaaaatg caataactac aatcatttta aaagattgaa tttggataaa tgtaatacta
     1681 aaaatgacaa aaaatactgc gcctgcattg acaccgaaaa gccctggtga ggctattggg
     1741 tttcgtgtaa gtgcttgcat caacaaacct gagacagcaa gggcagcacc agtcaataac
     1801 gcaatgattg ttctcgacgc ccgtgcacca gtgacaacat catgtaaatc gttttcacta
     1861 tcaaagttga ataacgcctg tatcaccgta cctggtgaca caagcgtatt tccaatcatt
     1921 aaacttaaga tagctactat tgcaagacat aaaccagcaa taacgatttg gtattttggt
     1981 ttaagtagca tcgtaaaact ccttaattat tttgattgtt tttcaatatt taacttttca
     2041 tataaatcgt caataagttt taatgaagat ttatatccgc cagctaagtt ccaagtgatt
     2101 tcatctaaat catcagatac ttggttgttt ttaactgcgt ctaaattttt ccactcttta
     2161 cttgaagtcc attcgctttc agtcttttta actaatgcag catctttcgc atttggatct
     2221 gattttacta caaaaatatg atcagcgttc attaatggaa tgctttcttt agatgtaagt
     2281 tggataatat ctttaccatt atcaacttgt ttttgtaagt ctttattacg tttgaatcct
     2341 aaatcattta agatttcacc agcatatcca ccagcataaa ttcttgtatg atcagcacgg
     2401 aagttaacaa ctgaagcttt caatggccat gcatctttat actttgcttt tgcatctttt
     2461 tggaatgcag ctactttatc atcgtacttt ttaagtaaat cttcagcttc tttttctttc
     2521 cctaaagctt tccccattaa cttagttgta tctttgaatt tgaaaactgt atcagtagaa
     2581 actgttggtg cgattttaga taattgatcg taaacttttt catttctaac ttttgacgcg
     2641 acaattaagt ccggttttaa tttagagatt tcctctaagt taggtgcagg ttcttgacct
     2701 acaatcttag tatcttttaa atcatttttt atgtattcga atttcggttt ttgtgtccat
     2761 gattctacag cacctacagg tttaacacct aaagatacag cgacgtcagt ggcaccttga
     2821 tatagcgtaa caacacgctt tggtttccct ttaatttcag ttgtacccat tgcatgttta
     2881 attgaagttg tttccttatc tttgttatca gatgattgtt tatttgaatt cccactacat
     2941 cctgctaaaa caagtaggaa agcaagcgta acaacaagca ttttaattac tttattcatt
     3001 gactaattag cctccttcgt gatgtatgac aatgagaatc attatcacga tttagtatga
     3061 attaaatttt ttcctaagtc aataaaatat ttatgattta catgcaactt ataattattt
     3121 gacatataaa tgcataaaaa atataatcct aattacttga tagtgagaat cattatcaat
     3181 taggtaacac acaatattat agaattttaa atttgaggag gaagcgcttt tgattgaaaa
     3241 aagtcaagca tgtcacgatt cattgttaga ttctgtaggg caaacaccta tggttcaact
     3301 tcatcaacta tttccgaaac atgaagtgtt tgcaaagtta gagtatatga atcctggagg
     3361 cagcatgaaa gatcgacctg ccaagtacat cattgaacat ggtattaaac atggtttaat
     3421 cactgagaat acacatttaa ttgaaagtac ttctggtaat ttaggcattg cgttggcaat
     3481 gatagctaaa atcaagggat taaaactcac gtgtgttgtt gatcctaaaa tatcaccaac
     3541 aaatttgaaa attattaaaa gttatggtgc caatgtagaa atggttgaag aacctgatgc
     3601 acatgggggt tatttaatga ctcgtattgc aaaggtgcaa gaactgttag ccactattga
     3661 cgatgcatat tggattaatc aatatgcgaa tgagttaaat tggcaatccc attatcatgg
     3721 tgcaggcaca gagattgttg aaacaattaa gcaacctata gattattttg tcgcgccagt
     3781 cagcacgaca ggtagcatta tgggtatgag tagaaaaata aaagaagtgc atccaaacgc
     3841 acaaattgtt gctgttgatg cgaaagggtc agtcattttt ggtgacaaac ctattaatag
     3901 agaattacct ggtatcggtg ctagtcgtgt acccgaaata ttgaatagat cagaaattaa
     3961 tcaagtgatc catgtagatg attatcaatc tgctttgggc tgtcgaaaac tgattgatta
     4021 tgaaggcata tttgccggag gttcaacagg ttcgattatt gcagcgattg agcagttgat
     4081 aacgtcaatt gaagaaggtg caacaattgt cacgatttta ccagatcgag gcgatcgtta
     4141 cttagattta gtttattcag atacatggtt agaaaaaatg aaatcaagac aaggagttaa
     4201 atcagaatga atagagagat gttgtattta aatagatcag atattgaaca agcgggaggt
     4261 aatcattcac aagtttatgt ggacgcatta acagaagcat taacagccca tgcgcacaat
     4321 gattttgtac aaccgcttaa gccgtattta agacaggatc ctgaaaatgg acacatcgca
     4381 gatcgaatta ttgcaatgcc aagtcatatc ggtggtgaac acgcaatttc aggtattaag
     4441 tggataggta gtaagcacga caatccatcg aaacgtaata tggagcgtgc aagtggcgtc
     4501 attattttga atgatccaga aacgaattat ccaattgcag ttatggaagc aagtttaatt
     4561 agtagtatgc gtactgcagc agtttcagtg attgcagcaa agcatttggc taaaaaagga
     4621 tttaaagact taacaatcat tggatgcggg ctaatcggag acaagcaatt acaaagtatg
     4681 ttagagcaat tcgatcatat tgaacgcgtg tttgtttacg atcaattctc tgaagcatgt
     4741 gcacgctttg ttgatagatg gcaacaacag cgtccggaaa ttaattttat tgcgacagaa
     4801 aatgctaaag aagcagtatc aaatggtgaa gtagtcatta catgtaccgt aacggatcaa
     4861 ccatacattg aatatgattg gttacaaaag ggtgcattta ttagcaacat ttctatcatg
     4921 gatgtgcata aagaagtctt tattaaagct gacaaagtcg tagtagatga ctggtcacaa
     4981 tgtaatcgag aaaagaaaac tattaaccaa ttggtgttag aaggtaaatt cagcaaagaa
     5041 gctcttcatg ctgaactagg acaacttgtg acaggtgaca taccaggacg tgaagacgat
     5101 gatgagatca tattacttaa tccgatgggt atggctatcg aagatatttc aagtgcttat
     5161 tttatttatc aacaggcaca acaacaaaat attgggacaa cattgaacct atattaagaa
     5221 tgcgaggtgt ctgaacattg cagaatcata cagcagtcaa tacagcacaa gcgataatat
     5281 taagagattt agttgatgca ttattatttg aagatatagc cggaattgta tcgaatagtg
     5341 agattactaa agaaaacgga caaacgcttt tgatatacga acgtgaaaca caacaaataa
     5401 agatacctgt ttattttagt gctttaaata tgtttcgtta cgaaagttca caaccaatta
     5461 cgatagaggg aagggtgtct aagcaacctt taacggcagc tgaattttgg caaacaattg
     5521 ctaatatgaa ttgtgatcta agtcatgaat gggaagtggc tcgcgttgaa gaaggactga
     5581 ctactgctgc cacacagctt gctaaacaat tatcagaatt agatttagcg tcacatcctt
     5641 ttgtgatgtc agagcagttt gcaagtttaa aagatcgtcc atttcatcca ttagctaaag
     5701 aaaaaagagg attaagagaa gcggattatc aagtgtatca agctgaatta aatcaatcat
     5761 ttcctttaat ggttgcagca gttaaaaaga cacatatgat tcatggcgat actgcaaata
     5821 tcgatgaatt agaaaatttg acagtaccta taaaagaaca agcgacagac atgttaaatg
     5881 atcaagggtt atcaatagat gactatgtac tatttccggt acatccttgg caatatcagc
     5941 atattctgcc gaacgtcttt gcgaaagaga ttagtgaaaa gttggttgta ctattaccgt
     6001 taaaatttgg agattatctg tcgtcttcaa gtatgcgttc attaattgat attggcgcac
     6061 cgtataacca tgtcaaagta ccatttgcaa tgcagtcatt aggcgcatta aggctaacgc
     6121 ctacgcgtta catgaaaaac ggagaacaag cagaacaatt attacgtcag cttatagaaa
     6181 aagatgaagc actagctaag tatgtcatgg tttgtgatga aacagcttgg tggtcatata
     6241 tgggtcaaga taatgatatt ttcaaagatc aattaggtca tctaactgtt cagctaagaa
     6301 agtatcccga agtgctagcc aaaaatgata cgcaacagct agtgtcaatg gcagcactcg
     6361 cggcaaatga tcgcacttta tatcaaatga tttgtggaaa agataatatt tctaaaaatg
     6421 atgtcatgac gttatttgaa gatatcgcgc aagtcttttt aaaggtaaca ctatcattta
     6481 tgcaatacgg cgcattacca gagttgcatg gtcaaaatat attgttgtca tttgaagatg
     6541 gacgtgtaca aaaatgcgtg ttacgtgatc atgatactgt cagaatttat aaaccatggc
     6601 taacagcaca tcagctttca ttgccgaagt atgtcgtcag agaagataca cctaatacgc
     6661 taattaatga ggatttggaa acattctttg cttattttca aacattagct gtatcggtaa
     6721 atctatatgc cattattgat gcaattcaag atttatttgg tgtaagtgag catgaactta
     6781 tgtcgttgtt aaaacaaatt ttaaaaaatg aagtggcaac tatttcctgg gttacaactg
     6841 atcagctagc tgtcagacac attttatttg ataaacagac gtggccattc aaacaaattt
     6901 tattaccatt gctatatcaa cgtgatagtg gtggaggtag tatgccttca ggtttaacta
     6961 ccgtaccaaa tccaatggtg acatatgatt aatcagtcta tatggcgcag taactttcgc
     7021 attttatggc tcagtcagtt tatagcgatt gctggactga cagtacttgt gccattattg
     7081 ccaatttata tggcatcact acaaaatcta tcagtcgtag aaatacagtt gtggagtggt
     7141 atagcgattg ctgctccagc tgtaacgacg atgatagctt cgccgatatg ggggaagcta
     7201 ggtgataaga tcagccgaaa atggatggtg ttaagagcgt tacttggttt ggcggtatgc
     7261 ttatttttaa tggcattgtg tacgacacca ttacagtttg tacttgtgag gttattgcag
     7321 ggactatttg gtggtgttgt tgatgcatca agtgcgtttg cgagtgcaga ggcgccagct
     7381 gaagatcgtg gaaaggtatt aggaagactg caaagttcag tcagcgcagg gtctcttgtg
     7441 gggccattaa ttggcggtgt tacagcttcg atattaggtt ttagtgcgtt actgatgagt
     7501 attgccgtta ttacttttat tgtctgtatt ttcggtgcat taaaattgat tgaaacgaca
     7561 catatgccaa aatcacaaac accaaatatt aataaaggta ttcgccgttc atttcaatgt
     7621 ctattatgca cacaacaaac atgtcgattt attatcgttg gcgttttagc aaactttgct
     7681 atgtatggca tgctaactgc attatcacca cttgcttcat cagtgaatca tacagcgata
     7741 gatgaccgta gtgtgattgg atttttacag tccgcatttt ggacggcttc gatattaagc
     7801 gcgcctttat ggggacgctt taatgataaa tcatatgtta aatcagtata tatatttgcc
     7861 acgattgcat gtggttgtag tgcgatactg caaggtttag cgacgaatat agagttttta
     7921 atggctgcaa gaatacttca aggattaaca tatagtgcat tgattcaaag tgtcatgttt
     7981 gttgtcgtga atgcgtgtca tcaacaactt aaaggcacat ttgttggaac gacgaacagt
     8041 atgttagttg ttggtcaaat tattggcagt cttagtggcg ctgccattac aagttatact
     8101 acaccagcta ctacgtttat cgttatgggc gtagtatttg cagtaagtag tttattttta
     8161 atttgttcaa ccatcactaa tcaaatcaac gatcacacat taatgaaatt atgggagttg
     8221 aaacaaaaaa gtgcaaaata aagaattaat acaacatgca gcgtatgcgg ctatcgaacg
     8281 cattttaaat gaatatttta gagaagaaaa tttatatcaa gtaccacctc aaaatcatca
     8341 atggtctata caattatcag agctcgaaac tttaacgggt gaatttcgct attggtctgc
     8401 gatggggcat catatgtatc atccagaggt atggcttatc gatggaaaaa gtaaaaaaat
     8461 aacaacttat aaagaagcaa ttgcgcgtat tttgcaacat atggctcaaa gtgcagataa
     8521 tcaaacggca gtgcaacaac atatggcgca aattatgtct gacatcgata atagcattca
     8581 tcgcacggca cgttatttgc aaagtaacac aatagactac gtagaggatc gttatatcgt
     8641 ttcagaacaa tctttatact taggtcatcc atttcatccg actcctaaga gtgcaagtgg
     8701 gttttcagaa gcagatttag agaaatatgc acccgaatgt catacatcat tccaattgca
     8761 ttatttagct gtgcatcaag atgttctgct cacgcgctat gtagaaggta aagaagatca
     8821 ggttgagaaa gtgttgtatc aattagcaga catagatata tcagagatac ccaaagattt
     8881 tattttatta ccaacacatc cttatcaaat caatgtgttg cgacagcatc cacagtatat
     8941 gcaatatagt gaacaaggtt taataaaaga ccttggcgtt tccggtgatt cagtgtaccc
     9001 gacgtcttcg gttagaactg tattttcaaa agcattaaac atttatttaa aattaccgat
     9061 acacgttaaa atcactaatt ttatacgtac gaatgacctt gaacagattg aacggacaat
     9121 tgatgccgcg caagttatcg catcagtcaa agatgaggtt gaaacacccc attttaaatt
     9181 gatgtttgaa gaaggatatc gtgcattgtt accgaatcca ttagggcaaa cagttgaacc
     9241 tgaaatggat ttattaacaa atagtgccat gattgttcgt gaagggatac cgaattacca
     9301 tgctgataaa gatattcatg tattggcgtc attatttgaa acgatgcctg attcaccgat
     9361 gtctaagtta tcacaagtga ttgagcaaag tggtttagca ccagaagcat ggcttgaatg
     9421 ttatttgaat cgtacattat tgccgatatt aaagctgttt agtaacacag gcattagtct
     9481 agaagcacat gtacaaaata cattaattga attaaaagat ggcatacccg acgtatgctt
     9541 tgtcagagat cttgaaggca tttgtctatc tagaacgatt gctactgaaa aacagcttgt
     9601 gccaaatgtt gtggcagcat caagccctgt tgtatatgca catgatgaag catggcatcg
     9661 tcttaaatat tacgttgtag taaatcactt aggacattta gtatcaacta ttggtaaagc
     9721 gactagaaat gaagttgtgt tatggcaact tgtagcgcat cgtcttatga cttggaaaaa
     9781 agaatacgcg aataacgcag tatttgttga ctgtgtagaa gatttatatc aaacgccgac
     9841 cattgcggct aaagcgaatt tgatgagtaa attgaatgat tgtggtgcaa accctattta
     9901 tacacatata ccaaatccaa tttgtcataa caaggaggta tcgtattgtg aatcaaacaa
     9961 ttcttaatcg tgtaaagact agagtgatgc accaactggt atcatcactt atttatgaga
    10021 atattgttgt gtataaagcg tcatatcaag acggtgtcgg tcattttaca atagaaggac
    10081 atgattcaga gtatcgtttt actgctgaaa agacacatag ctttgatcgt atacgtatca
    10141 catcaccaat tgagcgtgtc gtaggagatg aggcagatac aacaacagac tatacacaat
    10201 tattgagaga ggttgtattt acatttccta aaaatgatga aaagctagaa caatttattg
    10261 tcgagttatt acagacagaa ttaaaagata cgcaaagtat gcagtatcga gaatcaaacc
    10321 caccagcaac acctgagaca tttaacgact atgaatttta tgcgatggaa gggcatcagt
    10381 atcatccaag ttacaaatca cgtttaggat ttacgttgag tgataatttg aaatttggtc
    10441 ctgattttgt accaaacgtt aaactgcagt ggttagctat cgacaaagat aaagtagaaa
    10501 cgacggtatc aagaaatgtt gtagttaacg aaatgttacg tcaacaagtt ggcgataaga
    10561 cttatgaaca ttttgtacag caaattgaag catctggcaa acatgtaaat gatgttgaga
    10621 tgatacctgt acacccatgg cagtttgaac atgtcatcca agttgatttg gctgaagaaa
    10681 ggcttaatgg cacagtacta tggttagggg aaagtgatga gctatatcac cctcaacaat
    10741 cgattcgtac gatgtcgcca atagacacga caaaatatta tttaaaggta ccaataagta
    10801 taacgaacac ttcaacgaaa cgagtgttgg cgcctcatac aattgaaaat gcagcgcaaa
    10861 ttacggattg gttaaagcag atacagcaac aagatatgta tttaaaagat gaattaaaga
    10921 cagtttttct aggggaagtc ttaggacagt cttatttaaa tacacaactt tcgccttata
    10981 aacaaactca agtttatggt gcgttaggtg ttatatggcg tgaaaatata tatcatatgt
    11041 taatcgatga agaggatgcg ataccattta atgcacttta tgcaagtgat aaggatggtg
    11101 taccattcat tgaaaattgg attaaacaat atggttctga agcttggaca aagcaatttt
    11161 tagctgtagc gattcgtcca atgattcata tgctttatta tcacggtatt gcctttgaat
    11221 cgcatgcaca aaatatgatg ctcattcatg aaaatggttg gcctacacgt attgccttaa
    11281 aagatttcca tgatggtgtt cgttttaagc gtgagcattt aagtgaagct gcttcacacc
    11341 tgacattaaa gccaatgcca gaagcacata aaaaagtgaa tagtaattca tttattgaaa
    11401 cagatgacga acgtttagta cgcgactttt tacatgatgc atttttcttt attaatatcg
    11461 ccgaaatcat cttatttatt gaaaagcaat atggtatcga tgaggagctg caatggcaat
    11521 gggttaaagg catcatcgag gcgtatcaag aagcatttcc agagttgaat aactatcaac
    11581 atttcgattt gtttgaacct acgattcaag ttgaaaagtt aacgacacgt cgattattaa
    11641 gtgactccga gttaagaatt catcatgtta caaatccatt aggtgtagga ggtatcaatg
    11701 atgcaacaac tatctctgaa acatagatta aacaatggtg attcagttta tggcattttt
    11761 aattctatac cggacccatt gatgatcgag gttatcgcag caagcgggta tgactttgtt
    11821 gtgattgata cagaacacgt ggcgattaat gatgagacac tagcgcattt aattcgtgca
    11881 gctgaagcag cgcatattat accaattgta cgtgtcactg cagtgataga tagagatatc
    11941 attaaagtgt tggatatggg tgcgagaggt attattgtgc cacacgttaa agatcgtgag
    12001 acagttgagc atattgtgaa attaagtcgt tattacccgc aaggattaag aagtttgaat
    12061 ggtggtcgca tggcaagatt tggacgtaca ccattacttg atgcaatgga gatggctaat
    12121 gagcatatta tggtgattgc catgatagaa gatgttgaag gggttatggc cattgacgat
    12181 atagcacaag tcgaaggttt agacatgata gtcgaaggtg ccgcagattt atcgcagtca
    12241 cttggcatac catggcaaac gcgtgatgat caagtaacat cacatgttca acatattttt
    12301 gaagttgtga atgcacatgg taaacatttt tgtgcattac cacgtgaaga tgaagatatt
    12361 gcaaaatggc aggcacaagg tgtacaaaca tttattttag gtgatgatcg cggaaaaata
    12421 tatcgccatt taagtgcatc tctagcgacg tctaaacaga aaggggatga aggctaatgc
    12481 gtatagttca acctgttatt gaacaattaa aagcacaatc tcatccagtt tgtcattata
    12541 tctatgattt agtcggactg gaacatcatt tgcaacatat tacatcgtca ttgccgagta
    12601 attgtcaaat gtactatgca atgaaagcaa atagtgaacg aaaaatccta gatacaatta
    12661 gtcagtatgt tgaaggattc gaagttgcat ctcaaggtga aatagcaaaa ggtcttgctt
    12721 ttaaaccagc aaatcatatt atttttggtg gccctggtaa gacagacgag gaactaagat
    12781 atgcagtaag tgaaggtgtt cagcgtattc atgttgaaag tatgcatgaa ttacaacggc
    12841 taaatgccat cttagaagat gaagataaga cacaacacat tttattgcgt gttaatttag
    12901 caggaccatt tcccaatgca acgttgcata tggcaggacg cccaacacaa tttggtattt
    12961 ctgaagacga agttgatgat gtcattgaag ctgcgctcgc aatgccaaag attcatctag
    13021 atggatttca ttttcattct atttctaaca atttagactc gaatttacat gtcgatgtag
    13081 tgaaacttta ttttaaaaaa gcaaaggcat ggtctgaaaa acatcgattt ccactcaaac
    13141 atatcaatct tggtggtggc ataggcgtta actatgcaga tttaactaac caatttgaat
    13201 gggataattt tgtagaacgt tttaaaacac ttatcgttga gcaagaaatg gaagatgtga
    13261 cattgaactt tgaatgtggg cgctttattg tggcacatat tggttactat gtgacagaag
    13321 tgctagacat taagaaagta catggtgctt ggtatgccat attaagagga ggtacgcaac
    13381 aatttagact gccggtatct tggcaacata accatccttt tgacatttat cgctataagg
    13441 acaatccata ttcatttgaa aaagtttcaa tttcgagaca ggacacaacg ttagtcggtc
    13501 aattatgtac accgaaagat gtctttgcta gagaagtaca gatagacgca atcagtacag
    13561 gcgacgttat tgttttcaaa tatgcaggtg catacggatg gtctatttca catcacgatt
    13621 tcttaagcca tccacatcct gaatttattt atttaacaca aacaaaggag gatgaataac
    13681 tattgaatca tattcatgaa catttaaaat tggtaccagt agataagatt gatcttcacg
    13741 aaacattcga acctttaaga ttggaaaaaa cgaaaagtag tattgaagca gatgatttta
    13801 tacgtcatcc tattttagtg acagcgatgc aacatggtag atatatggtt atagatggtg
    13861 tgcatcggta tacaagtttg aaagcgttag gatgtaagaa agttccagtg caagaaatcc
    13921 atgaaacaca atattcaatt agtacatggc aacataaagt tccatttggt gtgtggtggg
    13981 aaacgttaca acaagaacat cgcttgccat ggactactga gacaagacaa gaagcgccat
    14041 ttattacaat gtgtcatggt gatacagaac aatatttgta tacaaaagat ttaggcgaag
    14101 cacattttca agtatgggaa aaggttgtcg caagttatag tggttgttgt tctgtagaga
    14161 gaattgcaca aggtacatat ccttgtcttt ctcaacaaga tgtactcatg aagtatcagc
    14221 cattgagtta taaggaaatt gaagcggttg ttcataaagg ggaaactgtg ccagcaggtg
    14281 tgacacgctt taatatttca ggacgatgtc ttaatcttca agtaccactg gcattactta
    14341 aacaagatga tgatgttgaa caactgcgca attggaagca gtttttagca gataagtttg
    14401 ccaatatgag atgctatact gaaaaagtat acttggtgga gcaatag
//
