BGC0000950

BGC0000950: napsamycin A biosynthetic gene cluster from Streptomyces sp. DSM 5940

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Location: 1 - 35,298 nt. (total: 35,298 nt).
This entry is originally from NCBI GenBank HQ287563.1.

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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General

Compounds

Genes

NRP

History

NRPS/PKS domains

KnownClusterBlast

General information about the BGC

MIBiG accession	BGC0000950
Short description	napsamycin A biosynthetic gene cluster from Streptomyces sp. DSM 5940
Status	Minimal annotation: no A minimal annotation only contains information on the BGC loci and one or more linked chemical product(s) Completeness: complete Whether the loci encodes everything needed for the pathway producing the compound(s)
Biosynthetic class(es)	NRP (Uridylpeptide) Other (Nucleoside)
Loci	NCBI GenBank: HQ287563.1
Compounds	napsamycin A napsamycin B napsamycin C napsamycin D mureidomycin A mureidomycin B
Species	Streptomyces sp. DSM 5940 [taxonomy]
References	Identification of a napsamycin biosynthesis gene cluster by genome mining. Kaysser L et al., Chembiochem (2011) PMID:21290549 Napsamycins, new Pseudomonas active antibiotics of the mureidomycin family from Streptomyces sp. HIL Y-82,11372. Chatterjee S et al., J Antibiot (Tokyo) (1994) PMID:8040059 Identification of mureidomycin analogues and functional analysis of an N-acetyltransferase in napsamycin biosynthesis. Tang X et al., Chembiochem (2013) PMID:24115404 Mureidomycins A-D, novel peptidylnucleoside antibiotics with spheroplast forming activity. II. Structural elucidation. Isono F et al., J Antibiot (Tokyo) (1989) PMID:2498274 Selective inhibition of the bacterial translocase reaction in peptidoglycan synthesis by mureidomycins. Inukai M et al., Antimicrob Agents Chemother (1993) PMID:8517724

Chemical products information

napsamycin A [synonyms: mureidomycin f]

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C₃₉H₄₈N₈O₁₂S₁

Chemical database entries
PubCHEM

napsamycin B

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C₄₀H₅₀N₈O₁₂S₁

Chemical database entries
PubCHEM

napsamycin C

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C₃₉H₅₀N₈O₁₂S₁

Chemical database entries
NPAtlas

napsamycin D

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C₄₀H₅₂N₈O₁₂S₁

Chemical database entries
NPAtlas

mureidomycin A

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C₃₈H₅₀N₈O₁₂S₁

Chemical database entries
PubCHEM

mureidomycin B

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C₃₈H₅₀N₈O₁₂S₁

Chemical database entries
PubCHEM
PubCHEM

List of genes involved in compound(s) production

Identifiers	Position	Product	Functions	Evidence	Extra
ADY76657.1 npsA	1 - 1035 (-)	transcriptional regulator	Regulation	Sequence-based prediction	copy AA seq copy Nt seq
ADY76658.1 npsB	1184 - 1714 (-)	N-acetyltransferase	Tailoring (Acetylation)	Knock-out	copy AA seq copy Nt seq
ADY76659.1 npsC	1889 - 2656 (+)	phosphatase	Unknown	Sequence-based prediction	copy AA seq copy Nt seq
ADY76660.1 npsP1	2783 - 3067 (+)	non-ribosomal peptide synthetase	Scaffold biosynthesis	Sequence-based prediction	copy AA seq copy Nt seq
ADY76661.1 npsP2	3115 - 4338 (+)	non-ribosomal peptide synthetase	Scaffold biosynthesis	Sequence-based prediction	copy AA seq copy Nt seq
ADY76662.1 npsD	4347 - 4571 (+)	MbtH-like protein	Scaffold biosynthesis	Sequence-based prediction	copy AA seq copy Nt seq
ADY76663.1 npsE	4568 - 5899 (+)	FAD-depending oxidoreductase	Precursor biosynthesis	Sequence-based prediction	copy AA seq copy Nt seq
ADY76664.1 npsP3	5930 - 8485 (-)	non-ribosomal peptide synthetase	Scaffold biosynthesis	Sequence-based prediction	copy AA seq copy Nt seq
ADY76665.1 npsF	8505 - 8870 (-)	cupin 2 domain-containing protein	Precursor biosynthesis	Sequence-based prediction	copy AA seq copy Nt seq
ADY76666.1 npsP4	8874 - 10535 (-)	non-ribosomal peptide synthetase	Scaffold biosynthesis	Sequence-based prediction	copy AA seq copy Nt seq
ADY76667.1 npsP5	10789 - 13350 (+)	non-ribosomal peptide synthetase	Scaffold biosynthesis	Sequence-based prediction	copy AA seq copy Nt seq
ADY76668.1 npsG	13605 - 14870 (+)	argininosuccinate lyase	Precursor biosynthesis	Sequence-based prediction	copy AA seq copy Nt seq
ADY76669.1 npsH	14867 - 15790 (+)	homoserine kinase	Precursor biosynthesis	Sequence-based prediction	copy AA seq copy Nt seq
ADY76670.1 npsI	15808 - 18075 (+)	DABA synthase	Precursor biosynthesis	Sequence-based prediction	copy AA seq copy Nt seq
ADY76671.1 npsJ	18092 - 19135 (+)	threonine aldolase	Precursor biosynthesis	Sequence-based prediction	copy AA seq copy Nt seq
ADY76672.1 npsK	19263 - 20105 (-)	phenylalanine 3-hydroxylase	Precursor biosynthesis	Sequence-based prediction	copy AA seq copy Nt seq
ADY76673.1 npsL	20112 - 20600 (-)	hypothetical protein	Unknown	Sequence-based prediction	copy AA seq copy Nt seq
ADY76674.1 npsP6	20727 - 22250 (-)	non-ribosomal peptide synthetase	Scaffold biosynthesis	Sequence-based prediction	copy AA seq copy Nt seq
ADY76675.1 npsM	22944 - 23765 (+)	hypothetical protein	Unknown	Sequence-based prediction	copy AA seq copy Nt seq
ADY76676.1 npsN	23760 - 24812 (-)	tRNA-dependent amide synthetase	Scaffold biosynthesis	Sequence-based prediction	copy AA seq copy Nt seq
ADY76677.1 npsO	24865 - 26148 (-)	exporter	Transport	Sequence-based prediction	copy AA seq copy Nt seq
ADY76678.1 npsP7	26411 - 27607 (+)	non-ribosomal peptide synthetase	Scaffold biosynthesis	Sequence-based prediction	copy AA seq copy Nt seq
ADY76679.1 npsQ	27635 - 28912 (+)	aminotransferase	Precursor biosynthesis	Sequence-based prediction	copy AA seq copy Nt seq
ADY76680.1 npsR	28937 - 29872 (+)	dioxygenase	Precursor biosynthesis	Sequence-based prediction	copy AA seq copy Nt seq
ADY76681.1 npsS	29994 - 30728 (+)	hypothetical protein	Unknown	Sequence-based prediction	copy AA seq copy Nt seq
ADY76682.1 npsT	30800 - 31138 (+)	hypothetical protein	Unknown	Sequence-based prediction	copy AA seq copy Nt seq
ADY76683.1 npsU	31135 - 32118 (+)	FMN-depending oxidoreductase	Unknown	Sequence-based prediction	copy AA seq copy Nt seq
ADY76684.1 npsP8	32220 - 33788 (-)	non-ribosomal peptide synthetase	Scaffold biosynthesis	Sequence-based prediction	copy AA seq copy Nt seq
ADY76685.1 npsV	33811 - 34581 (-)	methyltransferase	Tailoring (Methylation)	Sequence-based prediction	copy AA seq copy Nt seq

NRP-specific information

Subclass	Uridylpeptide
Cyclic?	no
Thioesterase genes	npsP5 (Type I)

NRP-synthases

Gene	Modules
npsP1	Module ?
npsP2	Module ? Condensation domain type: LCL
npsP3	Module ? Specificity: meta-tyrosine Evidence for specificity: Sequence-based prediction
npsP4	Module ? Condensation domain type: LCL
npsP5	Module ? Specificity: 2S,3S-diaminobutyric acid Evidence for specificity: Sequence-based prediction
npsP6	Module ? Specificity: meta-tyrosine Evidence for specificity: Sequence-based prediction
npsP7	Module ? Condensation domain type: LCL
npsP8	Module ? Specificity: methionine Evidence for specificity: Sequence-based prediction

Annotation changelog

MIBiG version	Submitter	Notes
1.0	Hidden contributor (ID: MQCAQSV425YQGLSERITRLP7K, no GDPR consent given).	Submitted
2.0	Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).	Migrated from v1.4 Updated compound(s) information (NPAtlas curation)
3.0	Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).	Corrected NRP module activity Updated bioactivity data Updated NRP substrate specificities
3.1	Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).	Update chemical activity to schema version 2.11

Detailed domain annotation

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