Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0000950.1:
Cluster 1NRP / Other135298

BGC0000950, napsamycin biosynthetic gene cluster from Streptomyces sp. DSM. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: napsamycin A
PubChem ID: 3037825
SMILES string: Copy to clipboard
Molecular formula: C39H48N8O12S
Average molecular mass: 852.917 Da
Molecular activity: Antibacterial
Molecular target: translocase I

Class-specific details

Biosynthetic class(es):
NRP / Other
Uridylpeptide (linear)
Thioesterase type:
Type I
Release / cyclization type:
Unknown

Nonribosomal peptide synthetases:
npsP1 (ADY76660.1)
Module x
A specificity: Unknown
Evidence for specificity: Unknown
C domain subtype: N/A
npsP2 (ADY76661.1)
Module x
A specificity: Unknown
Evidence for specificity: Unknown
C domain subtype: LCL
npsP3 (ADY76664.1)
Module x
A specificity: m-Tyrosine
Evidence for specificity: Sequence-based prediction
C domain subtype: N/A
npsP4 (ADY76666.1)
Module x
A specificity: Unknown
Evidence for specificity: Unknown
C domain subtype: LCL
npsP5 (ADY76667.1)
Module x
A specificity: (2S,3S)-2,3-Diaminobutyric acid
Evidence for specificity: Sequence-based prediction
C domain subtype: N/A
npsP6 (ADY76674.1)
Module x
A specificity: m-Tyrosine
Evidence for specificity: Sequence-based prediction
C domain subtype: N/A
npsP7 (ADY76678.1)
Module x
A specificity: Unknown
Evidence for specificity: Unknown
C domain subtype: LCL
npsP8 (ADY76684.1)
Module x
A specificity: Methionine
Evidence for specificity: Sequence-based prediction
C domain subtype: N/A
Other biosynthetic class:
Nucleoside

Gene cluster description

napsamycin (BGC0000950). Gene Cluster 1. Biosynthetic class = NRP/Other. GenBank HQ287563. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Domain annotation

Homologous known gene clusters

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Heterologous expression
MIxS-compliance:Unknown
Contact for this cluster:Leonard Kaysser (Eberhard Karls University Tuebingen)

Literature references

1. Kaysser L et al. (2011) Identification of a napsamycin biosynthesis gene cluster by genome mining. Chembiochem 12(3):477-87. doi: 10.1002/cbic.201000460. Epub 2010 Dec
2. Chatterjee S et al. (1994) Napsamycins, new Pseudomonas active antibiotics of the mureidomycin family from Streptomyces sp. HIL Y-82,11372. J Antibiot (Tokyo) 47(5):595-8.
3. Tang X et al. (2013) Identification of mureidomycin analogues and functional analysis of an N-acetyltransferase in napsamycin biosynthesis. Chembiochem 14(17):2248-55. doi: 10.1002/cbic.201300287. Epub 2013
4. Isono F et al. (1989) Mureidomycins A-D, novel peptidylnucleoside antibiotics with spheroplast forming activity. II. Structural elucidation. J Antibiot (Tokyo) 42(5):667-73.
5. Inukai M et al. (1993) Selective inhibition of the bacterial translocase reaction in peptidoglycan synthesis by mureidomycins. Antimicrob Agents Chemother 37(5):980-3.