BGC0000958: antimycin biosynthetic gene cluster from Streptomyces sp. S4
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Location: 81,953 - 106,578 nt. (total: 24,626 nt).
This entry is originally from NCBI GenBank NZ_FR873698.1.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
TTA codons
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Gene details
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General
Compounds
Genes
Biosynthesis
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0000958
Short description antimycin biosynthetic gene cluster from Streptomyces sp. S4
Status Quality: questionable
The quality level of this entry.

Status: active
The status of this entry.

Completeness: complete
Whether the entry covers everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • NRPS (Type I)
  • PKS (Type I)
Loci
NZ_FR873698.1
81953 - 106578
via Knock-out studies, Enzymatic assays, Heterologous expression, Gene expression correlated with compound production
Compounds
  • antimycin
Species Streptomyces sp. S4 [taxonomy]
References
Chemical products information
antimycin Evidence:
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C28H40N2O9
Chemical database entries
PubCHEM
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • STREPS4_RS11215
  • WP_003946493.1
 81953 - 82474 (-) RNA polymerase sigma factor
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    • STREPS4_RS11220
    • WP_026048085.1
    		 82523 - 83833 (-) condensation domain-containing protein
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      • STREPS4_RS11225
      • WP_010639240.1
      		 84401 - 93094 (+) non-ribosomal peptide synthetase
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        • STREPS4_RS11230
        • WP_010639241.1
        		 93087 - 96908 (+) type I polyketide synthase
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          • STREPS4_RS11235
          • WP_010639242.1
          		 96958 - 98184 (+) crotonyl-CoA carboxylase/reductase
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            • STREPS4_RS11240
            • WP_010639243.1
            		 98273 - 99751 (-) AMP-binding protein
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              • STREPS4_RS11245
              • WP_003946500.1
              		 99751 - 100008 (-) acyl carrier protein
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                • STREPS4_RS11250
                • WP_010639244.1
                		 100306 - 101361 (+) 1,2-phenylacetyl-CoA epoxidase subunit PaaA
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                  • STREPS4_RS11255
                  • WP_003946502.1
                  		 101358 - 101660 (+) 1,2-phenylacetyl-CoA epoxidase subunit PaaB
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                    • STREPS4_RS11260
                    • WP_010639245.1
                    		 101653 - 102441 (+) 1,2-phenylacetyl-CoA epoxidase subunit PaaC
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                      • STREPS4_RS11265
                      • WP_003946504.1
                      		 102438 - 102956 (+) 1,2-phenylacetyl-CoA epoxidase subunit PaaD
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                        • STREPS4_RS11270
                        • WP_010639247.1
                        		 102968 - 104071 (+) 2Fe-2S iron-sulfur cluster-binding protein
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                          • STREPS4_RS11275
                          • WP_010639249.1
                          		 104118 - 104894 (+) SDR family oxidoreductase
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                            • STREPS4_RS11280
                            • WP_003946507.1
                            		 104940 - 105788 (+) tryptophan 2,3-dioxygenase family protein
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                              • STREPS4_RS11285
                              • WP_010639251.1
                              		 105778 - 106578 (+) alpha/beta fold hydrolase
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                                Biosynthesis information

                                Biosynthetic modules

                                Name
                                1
                                Type
                                nrps-type1
                                Genes
                                STREPS4_RS11225
                                Substrates
                                threonine (evidence: Activity assay)
                                Integrated Monomers
                                Domains
                                condensation (Starter), adenylation
                                Name
                                2
                                Type
                                nrps-type1
                                Genes
                                STREPS4_RS11225
                                Substrates
                                pyruvic acid (evidence: Activity assay)
                                Integrated Monomers
                                Domains
                                condensation (LCL), adenylation
                                Name
                                3
                                Type
                                pks-modular
                                Genes
                                STREPS4_RS11230
                                Substrates
                                Integrated Monomers
                                Domains
                                acyltransferase, ketosynthase, acyltransferase, thioesterase, carrier (ACP)
                                Annotation changelog

                                Entry version: 4

                                Date
                                Changes
                                Submitters
                                Reviewers
                                2022-10-07
                                Update chemical activity to schema version 2.11
                                • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                                Entry version: 3

                                Date
                                Changes
                                Submitters
                                Reviewers
                                2022-09-15
                                Removed subcluster from chem_moiety
                                • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                2022-09-15
                                Corrected NRP module activity
                                • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                2022-09-15
                                Removed ketoreductase stereochemistry annotation from modules without ketoreductases
                                • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                2022-09-15
                                Reworked NRPS/PKS modules to match reference papers
                                • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                2022-09-15
                                Updated NRP substrate specificities
                                • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                                Entry version: 2

                                Date
                                Changes
                                Submitters
                                Reviewers
                                2019-10-16
                                Migrated from v1.4
                                • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                                Entry version: 1

                                Date
                                Changes
                                Submitters
                                Reviewers
                                2015-06-12
                                Submitted
                                • (ID: ROMZZBLG2S6RXTJZIS4SPKBK)
                                • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                Detailed domain annotation
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