BGC0000961 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000961 (Burkholderia thailandensis E264)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS,NRPS,hserlactone	1	36,595	bactobolin	NRPS:Type I+PKS	100%

BGC0000961 - Region 1 - NRPS,T1PKS,hserlactone

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Location: 1 - 36,595 nt. (total: 36,595 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX))
hserlactone: Autoind_synth

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 36,596
Label		BGC0000961

Detailed domain annotation

Selected features only

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Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000961	2.28	NRP, Polyketide	bactobolin	Burkholderia thailandensis E264
BGC0000375	0.86	NRP	indigoidine	Streptomyces chromofuscus
BGC0001561	0.84	NRP	curacomycin	Streptomyces curacoi
BGC0001925	0.79	Alkaloid	altemicidin, SB-203207, SB-203208	Streptomyces sp.
BGC0002710	0.78	NRP	metachelin C, metachelin A, metachelin A-CE, metachelin B, dimerumic acid 11-mannoside, dimerumic acid	Metarhizium robertsii ARSEF 23
BGC0001050	0.77	NRP, Polyketide	thalassospiramide A	Tistrella bauzanensis
BGC0000979	0.76	NRP, Polyketide	cylindrospermopsin	Aphanizomenon sp. 10E9
BGC0000980	0.76	NRP, Polyketide	cylindrospermopsin	Aphanizomenon sp. 22D11
BGC0000876	0.73	Other	neopolyoxin C	Streptomyces tendae
BGC0001132	0.70	NRP	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000961	1.00	NRP, Polyketide	bactobolin	Burkholderia thailandensis E264
BGC0000979	0.70	NRP, Polyketide	cylindrospermopsin	Aphanizomenon sp. 10E9
BGC0000980	0.70	NRP, Polyketide	cylindrospermopsin	Aphanizomenon sp. 22D11
BGC0001561	0.68	NRP	curacomycin	Streptomyces curacoi
BGC0002158	0.68	NRP, Polyketide	tenuazonic acid	Pyricularia oryzae 70-15
BGC0001050	0.67	NRP, Polyketide	thalassospiramide A	Tistrella bauzanensis
BGC0000375	0.66	NRP	indigoidine	Streptomyces chromofuscus
BGC0002710	0.65	NRP	metachelin C, metachelin A, metachelin A-CE, metachelin B, dimerumic acid 11-mannoside, dimerumic acid	Metarhizium robertsii ARSEF 23
BGC0001925	0.65	Alkaloid	altemicidin, SB-203207, SB-203208	Streptomyces sp.
BGC0000348	0.64	NRP	ergovaline	Epichloe festucae var. lolii

Similar known gene clusters from MIBiG 4.0

Module view

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 36595:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Gly) + (ohmal) + (Ala)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

BTH_II1226

Search NORINE for peptide: strict or relaxed

AMP-binding (59..454): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: DAFWLGGTFK
Nearest Stachelhaus code(s):: Val DAFWLGGTFK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

BTH_II1233

Search NORINE for peptide: strict or relaxed

AMP-binding (405..789): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLGVIWK
Nearest Stachelhaus code(s):: Gly DILQLGVIWK (82% 8Å match)
Stachelhaus code match:: 100% (strong)

BTH_II1234

PKS_AT (510..783): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 83.3%; Propionyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 126.0
inactive: 90.4
Methoxymalonyl-CoA: 57.0
Methylmalonyl-CoA: 46.4
Propionyl-CoA: 24.2
Isobutyryl-CoA: 20.4
fatty_acid: 16.9
Ethylmalonyl-CoA: 14.2
CHC-CoA: 13.6
trans-1,2-CPDA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

BTH_II1236

Search NORINE for peptide: strict or relaxed

AMP-binding (8..399): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: DLFNNALTYK
Nearest Stachelhaus code(s):: Ala DLFNNALTYK (85% 8Å match)
Stachelhaus code match:: 100% (strong)