BGC0000963: bleomycin biosynthetic gene cluster from Streptomyces verticillus
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Location: 1 - 77,457 nt. (total: 77,457 nt).
This entry is originally from NCBI GenBank AF210249.1.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
TTA codons
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Gene details
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General
Compounds
Genes
Biosynthesis
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0000963
Short description bleomycin biosynthetic gene cluster from Streptomyces verticillus
Status Quality: questionable
The quality level of this entry.

Status: active
The status of this entry.

Completeness: complete
Whether the entry covers everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • NRPS (Type I)
  • PKS (Type I)
  • saccharide (hybrid/tailoring)
Loci
AF210249.1
via Knock-out studies, Heterologous expression
Compounds
  • bleomycin
Species Streptomyces verticillus [taxonomy]
References
Chemical products information
bleomycin Evidence:
Copy SMILES
C55H84N17O21S3
Chemical database entries
PubCHEM
NPAtlas
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • AAG02342.1
 1199 - 2185 (-) SyrP-like protein
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    • AAG02343.1
    		 2182 - 3918 (-) peptide synthetase NRPS12
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      • AAG02344.1
      		 3915 - 4733 (-) hypothetical protein
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        • AAG02345.1
        		 4741 - 6024 (-) putative transporter
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          • AAG02346.1
          		 6021 - 6773 (-) thioesterase
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            • AAG02347.1
            		 6784 - 7797 (-) clavaminic acid synthase-like protein
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              • AAG02348.1
              		 7859 - 9214 (-) putative transporter
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                • AAG02349.1
                		 9211 - 17736 (-) peptide synthetase NRPS11-10
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                  • AAG02350.1
                  		 18307 - 18660 (-) PHNA-like protein
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                    • AAG02351.1
                    		 18823 - 19164 (+) metal-dependent regulatory protein
                    • Regulation
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                    • AAG02352.1
                    		 19161 - 20909 (+) putative transporter
                    • Transport
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                    • AAG02353.1
                    		 21367 - 22086 (+) phosphatidylserine decarboxylase
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                      • AAG02354.1
                      • blmXI
                      		 22127 - 24193 (+) peptide synthetase NRPS
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                        • AAG02355.1
                        • blmX
                        		 24406 - 30894 (+) peptide synthetase NRPS9-8
                        • Scaffold biosynthesis
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                        • AAG02356.1
                        • blmIX
                        		 30891 - 34091 (+) peptide synthetase NRPS7
                        • Scaffold biosynthesis
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                        • AAG02357.1
                        • blmVIII
                        		 34088 - 39613 (+) polyketide synthase
                        • Scaffold biosynthesis
                        • Knock-out
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                        • AAG02358.1
                        • blmVII
                        		 39610 - 43266 (+) peptide synthetase NRPS6
                        • Scaffold biosynthesis
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                        • AAG02359.1
                        • blmVI
                        		 43263 - 51290 (+) peptide synthetase NRPS5-4-3
                        • Scaffold biosynthesis
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                        • AAG02360.1
                        • blmV
                        		 51493 - 53430 (+) peptide synthetase NRPS3CT
                        • Scaffold biosynthesis
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                        • AAG02361.1
                        • blmG
                        		 53427 - 54404 (+) sugar epimerase BlmG
                        • Tailoring
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                        • AAG02362.1
                        • blmF
                        		 54418 - 55902 (+) hydroxylase BlmF
                        • Tailoring
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                        • AAG02363.1
                        		 55899 - 57815 (+) asparagine synthetase
                        • Precursor biosynthesis
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                        • AAG02364.1
                        • blmIV
                        		 57901 - 65781 (+) peptide synthetase NRPS2-1
                        • Scaffold biosynthesis
                        • Knock-out
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                        • AAG02365.1
                        • blmIII
                        		 65778 - 68585 (+) peptide synthetase NRPS0
                        • Scaffold biosynthesis
                        • Activity assay
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                        • AAG02366.1
                        		 68582 - 69601 (+) SyrP-like protein
                        • Regulation
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                        • AAG02367.1
                        • blmII
                        		 69598 - 70986 (+) peptide synthetase NRPS
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                          • AAG02368.1
                          		 70983 - 71546 (+) MbtH-like protein
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                            • AAG02369.1
                            • blmE
                            		 71618 - 72790 (+) sugar transferase BlmE
                            • Tailoring
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                            • AAG02370.1
                            • blmD
                            		 72787 - 74424 (+) carbamyltransferase BlmD
                            • Tailoring
                            • Knock-out
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                            • AAD42077.1
                            • blmI
                            		 74421 - 74693 (+) peptide synthetase NRPS type II-PCP
                            • Scaffold biosynthesis
                            • Activity assay
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                            • AAG02371.1
                            • blmC
                            		 74690 - 76186 (+) sugar synthase BlmC
                            • Activation / processing
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                            • AAG02372.1
                            		 76183 - 77457 (+) coproporphyrinogen III oxidase-like protein
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                              Biosynthesis information

                              Biosynthetic modules

                              Name
                              3
                              Type
                              nrps-type1
                              Genes
                              blmX
                              Substrates
                              asparagine (evidence: Sequence-based prediction)
                              Integrated Monomers
                              Domains
                              condensation (LCL), adenylation
                              Name
                              4
                              Type
                              nrps-type1
                              Genes
                              blmX
                              Substrates
                              histidine (evidence: Sequence-based prediction)
                              Integrated Monomers
                              Domains
                              condensation (LCL), adenylation
                              Name
                              5
                              Type
                              nrps-type1
                              Genes
                              blmIX
                              Substrates
                              alanine (evidence: Sequence-based prediction)
                              Integrated Monomers
                              Domains
                              condensation (LCL), adenylation
                              Name
                              6
                              Type
                              nrps-type1
                              Genes
                              blmVII
                              Substrates
                              threonine (evidence: Structure-based inference)
                              Integrated Monomers
                              Domains
                              condensation (LCL), adenylation
                              Name
                              1
                              Type
                              nrps-type1
                              Genes
                              blmVI
                              Substrates
                              serine (evidence: Sequence-based prediction)
                              Integrated Monomers
                              Domains
                              condensation (LCL), adenylation
                              Name
                              2
                              Type
                              nrps-type1
                              Genes
                              blmVI blmV
                              Substrates
                              asparagine (evidence: Sequence-based prediction)
                              Integrated Monomers
                              Domains
                              condensation (LCL), adenylation
                              Name
                              7
                              Type
                              nrps-type1
                              Genes
                              blmIV
                              Substrates
                              beta-alanine (evidence: Sequence-based prediction)
                              Integrated Monomers
                              Domains
                              condensation (LCL), adenylation
                              Name
                              8
                              Type
                              nrps-type1
                              Genes
                              blmIV
                              Substrates
                              cysteine (evidence: Sequence-based prediction)
                              Integrated Monomers
                              Domains
                              condensation (Heterocyclization), adenylation
                              Name
                              7
                              Type
                              pks-modular
                              Genes
                              blmVIII
                              Substrates
                              Integrated Monomers
                              Domains
                              acyltransferase, ketosynthase, acyltransferase, ketoreductase, methyltransferase, carrier (ACP)
                              Annotation changelog

                              Entry version: 4

                              Date
                              Changes
                              Submitters
                              Reviewers
                              2022-10-07
                              Update chemical activity to schema version 2.11
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                              Entry version: 3

                              Date
                              Changes
                              Submitters
                              Reviewers
                              2022-09-15
                              Linked new NPAtlas id for bleomycin (npatlas:NPA020036)
                              • (ID: H5KVDLSVQ3TJW7SKCDNBDEVC)
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                              2022-09-15
                              Corrected NRP module activity
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                              2022-09-15
                              Removed substrate from blmV gene
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                              2022-09-15
                              Fixed module numbers
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                              2022-09-15
                              Updated NRP substrate specificities
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                              Entry version: 2

                              Date
                              Changes
                              Submitters
                              Reviewers
                              2019-10-16
                              Migrated from v1.4
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                              Entry version: 1

                              Date
                              Changes
                              Submitters
                              Reviewers
                              2015-06-12
                              Submitted
                              • (ID: E2T2HCM4X7QXVS4LL4JVBPPK)
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                              Detailed domain annotation
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