BGC0000963: bleomycin biosynthetic gene cluster from Streptomyces verticillus
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Location: 1 - 77,457 nt. (total: 77,457 nt).
This entry is originally from NCBI GenBank AF210249.1.

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core biosynthetic genes
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Gene details
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General
Compounds
Genes
Polyketide
NRP
Saccharide
History
NRPS/PKS domains
General information about the BGC
MIBiG accession BGC0000963
Short description bleomycin biosynthetic gene cluster from Streptomyces verticillus
Status Minimal annotation: no
A minimal annotation only contains information on the BGC loci and one or more linked chemical product(s)

Completeness: complete
Whether the loci encodes everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • NRP (Glycopeptide)
  • Polyketide (Other)
  • Saccharide (hybrid/tailoring)
Loci NCBI GenBank: AF210249.1
Compounds
  • bleomycin
Species Streptomyces verticillus [taxonomy]
References
Chemical products information
Copy SMILES
bleomycin
Molecular Formula C70N18O26S12
Chemical database entries PubCHEM  
List of genes involved in compound(s) production
Identifiers Position Product Functions Extra
  • AAG02342.1
1199 2185 - SyrP-like protein
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  • AAG02343.1
2182 3918 - peptide synthetase NRPS12
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  • AAG02344.1
3915 4733 - hypothetical protein
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  • AAG02345.1
4741 6024 - putative transporter
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  • AAG02346.1
6021 6773 - thioesterase
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  • AAG02347.1
6784 7797 - clavaminic acid synthase-like protein
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  • AAG02348.1
7859 9214 - putative transporter
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  • AAG02349.1
9211 17736 - peptide synthetase NRPS11-10
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  • AAG02350.1
18307 18660 - PHNA-like protein
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  • AAG02351.1
18823 19164 + metal-dependent regulatory protein
  • Regulation (evidence: S)
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  • AAG02352.1
19161 20909 + putative transporter
  • Transport (evidence: S)
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  • AAG02353.1
21367 22086 + phosphatidylserine decarboxylase
  • Unknown (evidence: S)
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  • AAG02354.1
  • blmXI
22127 24193 + peptide synthetase NRPS
  • Unknown (evidence: S)
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  • AAG02355.1
  • blmX
24406 30894 + peptide synthetase NRPS9-8
  • Scaffold biosynthesis (evidence: S)
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  • AAG02356.1
  • blmIX
30891 34091 + peptide synthetase NRPS7
  • Scaffold biosynthesis (evidence: S)
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  • AAG02357.1
  • blmVIII
34088 39613 + polyketide synthase
  • Scaffold biosynthesis (evidence: K)
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  • AAG02358.1
  • blmVII
39610 43266 + peptide synthetase NRPS6
  • Scaffold biosynthesis (evidence: S)
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  • AAG02359.1
  • blmVI
43263 51290 + peptide synthetase NRPS5-4-3
  • Scaffold biosynthesis (evidence: S)
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  • AAG02360.1
  • blmV
51493 53430 + peptide synthetase NRPS3CT
  • Scaffold biosynthesis (evidence: S)
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  • AAG02361.1
  • blmG
53427 54404 + sugar epimerase BlmG
  • Tailoring (Epimerization) (evidence: S)
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  • AAG02362.1
  • blmF
54418 55902 + hydroxylase BlmF
  • Tailoring (Unknown) (evidence: S)
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  • AAG02363.1
55899 57815 + asparagine synthetase
  • Precursor biosynthesis (evidence: S)
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  • AAG02364.1
  • blmIV
57901 65781 + peptide synthetase NRPS2-1
  • Scaffold biosynthesis (evidence: K)
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  • AAG02365.1
  • blmIII
65778 68585 + peptide synthetase NRPS0
  • Scaffold biosynthesis (evidence: A)
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  • AAG02366.1
68582 69601 + SyrP-like protein
  • Regulation (evidence: S)
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  • AAG02367.1
  • blmII
69598 70986 + peptide synthetase NRPS
  • Unknown (evidence: S)
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  • AAG02368.1
70983 71546 + MbtH-like protein
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  • AAG02369.1
  • blmE
71618 72790 + sugar transferase BlmE
  • Tailoring (Glycosylation) (evidence: S)
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  • AAG02370.1
  • blmD
72787 74424 + carbamyltransferase BlmD
  • Tailoring (Acylation) (evidence: K)
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  • AAD42077.1
  • blmI
74421 74693 + peptide synthetase NRPS type II-PCP
  • Scaffold biosynthesis (evidence: A)
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  • AAG02371.1
  • blmC
74690 76186 + sugar synthase BlmC
  • Activation / processing (evidence: S)
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  • AAG02372.1
76183 77457 + coproporphyrinogen III oxidase-like protein
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Polyketide-specific information
Subclass Other
Cyclic? no
Polyketide-synthases
Genes Properties Modules
AAG02357.1 Synthase subclass: Modular type I
Module 7
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: AAG02357.1
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
Scaffold-modifying domains: Methylation
KR-domain stereochemistry: D-OH
NRP-specific information
Subclass Glycopeptide
Cyclic? no
NRP-synthases
Gene Modules
AAG02355.1
Module 8
Specificity: Histidine
Evidence for specificity:
  • Sequence-based prediction
Condensation domain type: LCL
Module 9
Specificity: Asparigine
Evidence for specificity:
  • Sequence-based prediction
Condensation domain type: LCL
AAG02356.1
Module 7
Specificity: Alanine
Evidence for specificity:
  • Sequence-based prediction
Condensation domain type: LCL
AAG02358.1
Module 6
Specificity: Threonine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
AAG02359.1
Module 3
Specificity: Asparigine
Evidence for specificity:
  • Sequence-based prediction
Condensation domain type: LCL
Module 4
Specificity: Serine
Evidence for specificity:
  • Sequence-based prediction
Condensation domain type: LCL
Module 5
Condensation domain type: Starter
AAG02360.1
Module 3
Specificity: Asparigine
Evidence for specificity:
  • Sequence-based prediction
Condensation domain type: LCL
AAG02364.1
Module 0
Specificity: Cysteine
Evidence for specificity:
  • Sequence-based prediction
Condensation domain type: Heterocyclization
Modification domains: Oxidation
Module 1
Specificity: Cysteine
Evidence for specificity:
  • Sequence-based prediction
Condensation domain type: LCL
Modification domains: Unknown
Module 2
Specificity: Beta-alanine
Evidence for specificity:
  • Sequence-based prediction
Condensation domain type: LCL
Saccharide-specific information
Subclass hybrid/tailoring
Annotation changelog
MIBiG version Notes
2.0
  • Migrated from v1.4
Detailed domain annotation
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