BGC0000964: burkholdac A biosynthetic gene cluster from Burkholderia thailandensis E264
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Location: 2,655,707 - 2,694,284 nt. (total: 38,578 nt).
This entry is originally from NCBI GenBank CP000086.1.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
TTA codons
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Gene details
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General
Compounds
Genes
Polyketide
NRP
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0000964
Short description burkholdac A biosynthetic gene cluster from Burkholderia thailandensis E264
Status Minimal annotation: no
A minimal annotation only contains information on the BGC loci and one or more linked chemical product(s)

Completeness: complete
Whether the loci encodes everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • NRP (Cyclic depsipeptide)
  • Polyketide (Ansamycin)
Loci NCBI GenBank: CP000086.1
Compounds
  • burkholdac A
  • burkholdac B
Species Burkholderia thailandensis E264 [taxonomy]
References
Chemical products information
burkholdac A [synonyms: thailandepsin c]
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C22H35N3O6S3
burkholdac B
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C23H37N3O6S3
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • BTH_I2357
  • ABC39254.1
 2655707 - 2656543 (-) thioesterase type II
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  • BTH_I2358
  • ABC38006.1
 2656540 - 2657460 (-) lipase/esterase
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  • BTH_I2359
  • ABC38333.1
 2657527 - 2658501 (-) pyridine nucleotide-disulphide oxidoreductase, class II
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  • BTH_I2360
  • ABC38000.1
 2658514 - 2660745 (-) nonribosomal peptide synthetase, putative
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  • BTH_I2361
  • ABC38018.1
 2660742 - 2661710 (-) phosphotransferase enzyme family protein, putative
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  • BTH_I2362
  • ABC37307.1
 2661707 - 2662879 (-) acyl-CoA dehydrogenase domain protein
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  • BTH_I2363
  • ABC38101.1
 2662872 - 2667449 (-) polyketide synthase
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  • BTH_I2364
  • ABC36785.1
 2667446 - 2678398 (-) peptide synthetase, putative
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  • BTH_I2365
  • ABC36687.1
 2678395 - 2681949 (-) polyketide synthase
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  • BTH_I2366
  • ABC38737.1
 2681946 - 2686628 (-) polyketide synthase
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  • BTH_I2367
  • ABC39418.1
 2686625 - 2691724 (-) dihydroaeruginoic acid synthetase
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  • BTH_I2368
  • ABC36453.1
 2692046 - 2692255 (-) hypothetical protein
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  • BTH_I2369
  • ABC37158.1
 2693178 - 2694284 (+) transcriptional regulator, AraC family domain protein
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Polyketide-specific information
Subclass Ansamycin
Starter unit Malonyl-CoA
Cyclic? yes
Release type
  • Macrolactonization
Polyketide-synthases
Genes Properties Modules
BTH_I2366
+
BTH_I2367
+
BTH_I2363
+
BTH_I2365 		Synthase subclass: Trans-AT type I
Module 0
Specificity: Malonyl-CoA
Genes: BTH_I2367
Core domains: CoA-ligase
Module 2
Genes: BTH_I2366
Core domains: Ketosynthase, Dehydratase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: Unknown
Module 3
Genes: BTH_I2365
Core domains: Ketosynthase, Dehydratase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: Unknown
Module 7
Genes: BTH_I2363
Core domains: Ketosynthase, Ketoreductase, Thiolation (ACP/PCP), Epimerization
KR-domain stereochemistry: Unknown
NRP-specific information
Subclass Cyclic depsipeptide
Cyclic? yes
Release type
  • Macrolactamization
NRP-synthases
Gene Modules
BTH_I2367
Module 1
Specificity: cysteine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: Heterocyclization
BTH_I2364
Module 4
Specificity: methionine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
Module 5
Specificity: cysteine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: Dual
Module 6
Specificity: isoleucine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: DCL
BTH_I2360
Module 8
Condensation domain type: DCL
Annotation changelog
MIBiG version Submitter Notes
1.0
  • Hidden contributor (ID: WBX64IC5EUFODOV2ECKGLTGC, no GDPR consent given).
  • Submitted
2.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Migrated from v1.4
3.0
  • Hidden contributor (ID: H5KVDLSVQ3TJW7SKCDNBDEVC, no GDPR consent given).
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Add new compound: burkholdac B
  • Corrected NRP module activity
  • Removed ketoreductase stereochemistry annotation from modules without ketoreductases
  • Removed annotations for missing genes
  • Updated bioactivity data
  • Updated NRP substrate specificities
3.1
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Update chemical activity to schema version 2.11
Detailed domain annotation
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